--- Log opened Thu Mar 23 00:00:06 2017
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04:42 < kanzure> bloop
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07:04 < JayDugger> splat
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07:08 < JayDugger> entity8421[m] Org-mode
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07:39 < kanzure> "Rapid sequential in situ multiplexing with DNA-exchange-imaging" http://biorxiv.org/content/early/2017/03/20/112227 https://twitter.com/eboyden3/status/844478847290671104
07:39 < kanzure> "To decipher the molecular mechanism of biological function, it is critical to map the molecular composition of individual cells in the context of their biological environment in situ. Immunofluorescence (IF) provides specific labeling for molecular profiling. However, conventional IF methods have finite multiplexing capabilities due to spectral overlap of the fluorophores. Various sequential ...
07:40 < kanzure> ...imaging methods have been developed to circumvent this spectral limit, but are not widely adopted due to the common limitation of requiring multi-rounds of slow (hours to overnight in practice) immunostaining. To overcome this speed restriction and develop a practical platform for rapid in situ multiplexing, we describe here DNA-Exchange-Imaging, which allows single-step immunostaining with DN...
07:40 < kanzure> ...A-barcoded antibodies, followed by rapid (minutes) buffer exchange of fluorophore-bearing DNA imager strands. By eliminating the need for multiple rounds of immunostaining, DEI enables rapid spectrally unlimited sequential imaging. The programmability of DNA-Exchange-Imaging further allows us to adapt it to diverse microscopy platforms (with Exchange-Confocal, Exchange-SIM, Exchange-STED, and ...
07:40 < kanzure> ...Exchange-PAINT demonstrated here), achieving highly multiplexed in situ protein visualization in diverse samples (including neuronal and tumor cells as well as fresh-frozen or paraffin-embedded tissue sections) and at multiple desired resolution scales (from ~300 nm down to sub-20-nm). Validation highlights include 8-target imaging using single-channel Exchange-Confocal in tens of micron thick...
07:40 < kanzure> ... retina tissue sections in 2-3 hours (as compared to days required in principle by previous methods using comparable equipment), and 8-target super-resolution imaging with ~20 nm resolution using Exchange-PAINT in primary neurons. These results collectively suggest DNA-Exchange as a versatile, practical platform for rapid, highly multiplexed in situ imaging, potentially enabling new applicatio...
07:40 < kanzure> ...ns ranging from basic science, to drug discovery, and to clinical pathology."
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10:31 < nmz787_i> kanzure: that lego bot is pretty cool, I like that it has some material for teaching it to younger folks
10:44 < kanzure> immunostaining with DNA-barcoded antibodies is way cooler :)
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11:23 < nmz787_i> welp, got the laser cutter... now I need to find somewhere reasonable to put it
11:24 < nmz787_i> guess the garage
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11:41 < kanzure> roof
11:44 < nmz787_i> rain != good
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14:11 < nmz787_i> damn, I need a graduated cylinder to measure like 42mL of alcohol... and I don't have one :/ things will be delayed unless a craigslist person responds, a friend has one I can borrow, or Amazon Prime comes through (since I haven't ordered, probably wouldn't be til Sunday or Monday)
14:13 < chris_99> homebrew shops probably have measuring cylinders you could use, if theres any near you
14:14 < nmz787_i> ooo
14:14 < nmz787_i> yes!
14:14 < nmz787_i> <10 mins drive or so
14:14 < chris_99> :)
14:14 < nmz787_i> ;)
14:15 < darsie> nmz787_i: Use a scale.
14:15 < chris_99> mm that's a good idea
14:16 < nmz787_i> hmm, I do have a micropipette that can do 1mL
14:16 < nmz787_i> do that a few times to calibrate for lab temp, etc
14:16 < nmz787_i> then add to what I need
14:17 < nmz787_i> darsie: thanks :)
14:17 < nmz787_i> also apparently 2 brew shops within about the same distance
14:22 < nmz787_i> yeah they want around $13, when on Amazon I can get a set of 4 for $19 with a bottle brush
14:23 < nmz787_i> guess I'll order online and go with the scale for now
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21:08 < kanzure> hrm.
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--- Log closed Fri Mar 24 00:00:07 2017