--- Log opened Tue Apr 04 00:00:17 2017
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01:57 < kanzure> hmph
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02:44 < kanzure> 02:40 < kanzure> here are some old logs about extension blocks https://www.reddit.com/r/Bitcoin/comments/63b8lb/purse_extension_blocks_ready_for_liftoff/dft4nj1/?context=1
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07:53 < kanzure> blort
08:00 < kanzure> https://techcrunch.com/2017/04/04/data-collective-and-synbiobeta-founder-jon-cumbers-create-a-seed-stage-biotech-fund/
08:00 < kanzure> http://www.dcvc.com/
08:01 < kanzure> i thought his name was john cumbers, why is the permalink slug using jon
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09:56 < kanzure> stem cell banking service that takes bitcoin payments https://foreverlabs.com/
09:57 < kanzure> from this user https://www.reddit.com/user/fangolo
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11:48 < kanzure> "Dietary restriction protects from age-associated DNA methylation and induces epigenetic reprogramming of lipid metabolism" https://genomebiology.biomedcentral.com/articles/10.1186/s13059-017-1187-1
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11:56 < kanzure> "Chemical screening identifies ATM as a target for alleviating senescence" http://www.nature.com/nchembio/journal/vaop/ncurrent/full/nchembio.2342.html
11:57 < kanzure> "I would suggest you reconsider; the attachment of ATM to the lysosome, immediately raised alarm bells for me. Of course that's because that's where the mTOR super-complex forms, "Once on the lysosome, mTORC1 forms a four-component super complex with v-ATPase, Ragulator, and Rag, as suggested by co-immuno-precipitation of mTORC1 with at least Ragulator and Rag (Sancak et al., 2010)." See attac...
11:57 < kanzure> ...hed paper - and as mTOR(C1) has important roles in directing metabolism that's not such a surprise that it would be involved (it's known) in senescence. As for the ATM kinase having a single function- that would make it practically unique among proteins - for example, the common enzyme peroxiredoxin normally inactivates peroxides, but when it is inactivated, it becomes a chaperone.  Several me...
11:57 < kanzure> ...tabolic enzymes become transcription factors and the unassuming simple cell-to-cell adhesion molecule beta-catenine becomes one of the most important transcription co-factors when translocated to the nucleus. So ATM is a kinase, and has the role of activating enzymes for double-strand break repair and apparently also controls autophagy (where its attachment to the lysosome would seem convenien...
11:57 < kanzure> ...t),  and suspected to influence mitochondrial homeostasis. We don't know all the  proteins it interacts with (though we do know a couple of dozen, including p53 and BRCA1 and RAD51 So while it does seem counter-intuitive that this enzyme involved in DNA repair should be inactivated to produce rejuvenation. I can imagine a situation where not repairing double strand breaks or responding to them...
11:58 < kanzure> ... at all (for instance by not PARPylating them and thus saving on NAD+)might result in rejuvenation. At this point we're not even sure of where mTOR operates and we don't know all the substates of ATM."
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11:59 < kanzure> "Where is mTOR and what is it doing there?" https://www.researchgate.net/profile/Charles_Betz/publication/259566905_Where_is_mTOR_and_what_is_it_doing_there/links/0046352b2e0ffad43b000000.pdf (2013)
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12:01 < kanzure> ATM paper http://diyhpl.us/~bryan/papers2/longevity/Chemical%20screening%20identifies%20ATM%20as%20a%20target%20for%20alleviating%20senescence%20-%202017.pdf
12:03 < ebowden> Who wrote that comment you pasted?
12:04 < ebowden> Is it a letter to the editor?
12:04 < kanzure> harold
12:05 < kanzure> http://www.programmed-aging.org/theory-3/Katcher.html
12:05 < ebowden> Ok. Is it a letter to the editor?
12:05 < ebowden> Ah.
12:05 < kanzure> no
12:05 < ebowden> Yes, I saw the link.
12:05 < kanzure> http://ageing-research.blogspot.com/2011/03/guest-blog-harold-katcher-is-prevention.html
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12:28  * kanzure wonders about http://www.programmed-aging.org/
12:29 < kanzure> "Prof. Harold Katcher at the University of Maryland has proposed that Heterochronic Plasma Exchange (HPE) could be used to study the aging program in humans and other mammals and might form the basis of an anti-aging treatment. The company Turritopsis Corp. has been founded to carry out this activity."
12:29 < kanzure> turritopsis corp?
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12:56 < TMA> kanzure: probably "watchtower" in graecolatin; are they related to jehova's witnesses?
13:05 < kanzure> where was that recombinase combinator computer science theory paper thingy, did we lose that?
13:06 < docl> Nanofabrication Enables "Particle-Accelerator-on-a-Chip" Technology http://spectrum.ieee.org/nanoclast/semiconductors/devices/nanofabrication-enables-acceleratoronachip-technology
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13:37 < kanzure> do we have anything to ask mega to synthesize? https://groups.google.com/d/msg/diybio/FjOMXRE0m3A/6ThoqGH2DQAJ
13:38 < kanzure> he needs 20x fragments each between 130-1800 bp
13:38 < kanzure> not many proteins will fit in that space i guess
13:38 < kanzure> ~300 amino acids
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14:08 < kanzure> i preferred the animated origin story for ghost in the shell https://www.youtube.com/watch?v=Xgico_Psbgk
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16:04 < kanzure> .title https://www.youtube.com/watch?v=FC6kl_kLFEo
16:04 < yoleaux> SKI School: The Combinator Calculus Demystified - YouTube
16:07 < kanzure> https://www.fightaging.org/archives/2017/03/methuselah-foundation-launches-methuselah-fund/
16:08 < kanzure> https://www.methuselahfund.com/
16:09 < kanzure> http://apollo.vc/
16:09 < kanzure> https://www.fightaging.org/archives/2016/10/unity-biotechnology-raises-116m-for-senescent-cell-clearance-development/
16:10 < kanzure> http://oisinbio.com/
16:11 < kanzure> http://openlongevity.org/
16:11 < kanzure> https://www.fightaging.org/archives/2017/03/mikhail-batin-and-the-open-longevity-project/
16:11 < kanzure> http://scienceagainstaging.com/school
16:13 < abetusk> https://www.youtube.com/watch?v=pSls9L_h3Q0
16:13 < abetusk> .title
16:13 < yoleaux> OpenDrop Digital Microfludics - Speed Test - YouTube
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16:14 < kanzure> http://aginglab.science/en/
16:15 < kanzure> https://nam.edu/initiatives/grand-challenges-in-health-and-medicine/longevity-grand-challenge-prizes-awards/
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16:16 < kanzure> "A conserved NAD binding pocket that regulates protein-protein interactions during aging" http://science.sciencemag.org/content/355/6331/1312 https://www.sciencedaily.com/releases/2017/03/170323141340.htm
16:19 < nmz787_i> abetusk: can you buy the openDrop?
16:26 < abetusk> nmz787_i, not as far as I can tell
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16:57 < kanzure> .tw https://twitter.com/SynBioBeta/status/849207393103884288
16:57 < yoleaux> .@johncumbers: "Over $1 Billion USD was invested in synthetic biology companies in 2016: http://bit.ly/2oEbCKM #synbio #engbio #SBBUK17 " https://pbs.twimg.com/media/C8j9n1bUIAIrXgo.png (@SynBioBeta)
16:59 < kanzure> .tw https://twitter.com/sachsdaniel/status/849308865778913281
16:59 < yoleaux> My takeaway from day 1 of @SynBioBeta #SBBUK17: DNA synthesis is HOT (and nobody gives away their trade secrets, yet. Duh!) (@sachsdaniel)
17:00 < kanzure> .tw https://twitter.com/DrTomEllis/status/849285699270582275
17:00 < yoleaux> Very interesting sneak peak on template-free DNA Polymerase for enzymatic DNA synthesis from @DNAScript #SBBUK17 (@DrTomEllis)
17:01 < kanzure> http://www.dnascript.co/
17:03 < kanzure> hmm so what is their technique.
17:07 < kanzure> .tw https://twitter.com/rosscloney/status/849286701147181057
17:07 < yoleaux> Want to make DNA? Get an engineered polymerase to do it. Template free synthesis from @DNAScript #SBBUK17 (@rosscloney)
17:07 < kanzure> http://labiotech.eu/dna-script-thomas-ybert-interview/
17:08 < kanzure> "Today, most of the innovation in DNA synthesis focuses on the hardware side. DNA Script however focuses on changing the very basis of the conventional chemical technology to replace it by a biochemical synthesis using enzymes."
17:08 < kanzure> "the manufacturing process will be drastically simplified and will occur in aqueous media instead of harsh organic solvents"
17:30 < kanzure> labcyte, opentrons making appearances.. not unexpected.
17:32 < kanzure> "joining the circulatory system of two rats" (2015) http://www.nature.com/news/ageing-research-blood-to-blood-1.16762 (for aging/blood rejuvenation stuff)
17:33 < kanzure> "Regulation of life span by the gut microbiota in the short-lived african turquoise killifish" http://biorxiv.org/content/early/2017/03/27/120980 http://www.nature.com/news/young-poo-makes-aged-fish-live-longer-1.21770
17:33 < kanzure> "It may not be the most appetizing way to extend life but researchers have shown for the first time that older fish live longer after they consumed microbes from the poo of younger fish. The findings were posted to the bioRxiv.org preprint server on 27 March"
17:34 < kanzure> young poo rejuvenation :)
18:03 < kanzure> chrome extension that tries to find paper pdf file http://unpaywall.org/  seems to only work for papers where authors have already uploaded and google scholar has indexed (or on researchgate etc)
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18:40 < fenn> "Turritopsis dohrnii, the immortal jellyfish" can hit the reset button and revert to an earlier developmental stage if it is injured or otherwise threatened.
18:40 < kanzure> but it's about blood
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18:52 < fenn> it's probably about creating progenitor cells
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18:53 < fenn> "turritopsis corp was founded to pursue heterochronic plasma exchange research"
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18:55 < fenn> maybe they just think it sounds cool
18:56 < fenn> love the left handed DNA helix on ybert interview
19:00 < kanzure> which one is the ybert interview
19:00 < fenn> http://labiotech.eu/dna-script-thomas-ybert-interview/
19:00 < fenn> both pictures are wrong actually
19:00 < kanzure> oh, that interview was worthless except for two sentences, and even then..
19:18 < yashgaroth> so it's just TdT adding protected nucleotides, then they deprotect and repeat the cycle? how is that even better than phosphoramidite
19:18 < yashgaroth> admittedly the patent was in french so I may have missed something
19:19 < kanzure> oh i can pretend to read french, gimme the link
19:20 < yashgaroth> http://www.google.us/patents/WO2015159023A1?cl=en admittedly it's auto-translated which just mindfucks the grammar
19:23 < kanzure> oh they do mention TdT
19:23 < yashgaroth> or like telomerase and other template-independent enzymes, but they only list a protocol for tdt
19:24 < yashgaroth> and no patented mutations, so either it's wild-type, or they're keeping them secret (though they don't mention modifying it), or are gene patents illegal in france idk
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19:33 < kanzure> i mean we saw a tdt method but it was also using phosphoramidites or something
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19:45 < nmz787_> sup
19:46 < nmz787_> .tell chris_99: you may see this by the time you get this message: http://hackaday.com/2017/04/04/edm-for-the-cheap-and-adventurous/
19:46 < yoleaux> nmz787_: What kind of a name is "chris_99:"?!
19:46 < nmz787_> .tell chris_99 : you may see this by the time you get this message: http://hackaday.com/2017/04/04/edm-for-the-cheap-and-adventurous/
19:46 < yoleaux> nmz787_: I'll pass your message to chris_99.
19:46 < nmz787_> yoleaux: .issue support terminating .tell commands at : character
20:18 < kanzure> "Proc?d? de synth?se d'acides nucl?iques, notamment d'acides nucl?iques de grande longueur, utilisation du proc?d? et kit pour la mise en oeuvre du proc?d?"
20:18 < kanzure> that's what this channel has been missing, more indecipherable gibberish
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23:04 < nmz787_> "We had our first sequencing run go off without a hitch yesterday and have some Ebola samples on the MiSeq now." "The sequencer is 100% solar powered, because it is the most reliable and cleanest power."
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--- Log closed Wed Apr 05 00:00:17 2017