--- Log opened Sun Apr 30 00:00:40 2017
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03:36 < kanzure> p. sure RNA is annoying to work with
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07:47 < kanzure> trouble with mutantpedia is that you still have to figure out what questions to ask people
07:47 < kanzure> or you have them submit their own unstructured phenotype data
07:47 < kanzure> "oh that's not normal?"
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08:12 < kanzure> "I have managed to obtain a few mg of the FOXO4-DRI peptide as used in the recent paper by Baar et al: "Targeted Apoptosis of Senescent Cells Restores Tissue Homeostasis in Response to Chemotoxicity and Aging". I wish to test this peptide on myself."
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08:19 < kanzure> http://gdf11rejuvenation.com/
08:20 < kanzure> "Human umbilical cord plasma proteins revitalize hippocampal function in aged mice" http://www.nature.com/nature/journal/v544/n7651/full/nature22067.html
08:25 < kanzure> oh that's the TIMP2 stuff again.
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08:51 < kanzure> rs480 and modafinil response https://www.reddit.com/r/afinil/comments/38smx5/modafinil_and_rs4680_snp_if_you_have_genome_data/ "Met/Met=AA; High dopamine in prefrontal cortex; Val/Met=AG; Higher dopamine in prefrontal cortex (not as high as AA). Val/Val= GG; Lower dopamine in prefrontal cortex."
08:52 < kanzure> er.. rs4680
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09:24 < gnusha_> https://secure.diyhpl.us/cgit/diyhpluswiki/commit/?id=f21f9b5a Bryan Bishop: most SNPs from #lesswrong irc logs >> http://diyhpl.us/diyhpluswiki/genetic-modifications/
09:39 < streety> "Tissue inhibitor of metalloproteinases 2 (TIMP2), a blood-borne factor  enriched in human cord plasma, young mouse plasma, and young mouse  hippocampi," . . . and the urine after acute kidney injury http://www.astutemedical.com/us/products/nephrocheck-test/
09:41 < kanzure> so what you're saying is that we should punch all the demented old people straight in the kidneys?
09:42 < streety> I'm saying it might not be universally beneficial
09:44 < streety> not that it's use as a diagnostic for kidney injury implies that it is a negative there. But there is much we don't know about its role throughout the body
09:46 < andytoshi> https://www.cfa.harvard.edu/~loeb/habitable.pdf
09:46 < andytoshi> .title
09:46 < yoleaux> andytoshi: Sorry, that doesn't appear to be an HTML page.
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09:46 < andytoshi> "The habitable epoch of the early Universe"
09:54 < kanzure> "In the redshift range 100,(1+z),137, the cosmic microwave background (CMB) had a temperature of 273-373 K (0-100 ?C), allowing early rocky planets (if any existed) to have liquid water chemistry on their surface and be habitable, irrespective of their distance from a star. In the standard ?CDM cosmology, the first star-forming halos within our Hubble volume started collapsing at these r...
09:55 < kanzure> ...edshifts, allowing the chemistry of life to possibly begin when the Universe was merely 10-17 million years old. The possibility of life starting when the average matter density was a million times bigger than it is today is not in agreement with the anthropic explanation for the low value of the cosmological constant."
09:56 < andytoshi> .title https://www.reddit.com/r/askscience/comments/37z0cv/i_have_some_questions_about_something_i_read/
09:56 < yoleaux> I have some questions about something I read about: the habitable epoch of the early universe. Could you answer one or more of them for me? : askscience
09:57 < andytoshi> (reddit listing many reasons that the state of the universe at that time was not really compatible with life)
10:19 < kanzure> some deep learning speech recognition things with tensorflow https://github.com/zzw922cn/Automatic_Speech_Recognition
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11:00 < nmz787_> kanzure: I just read a blurb on electroporating siRNA
11:01 < nmz787_> http://www.bio-rad.com/webroot/web/pdf/lsr/literature/Bulletin_5687.pdf
11:01 < nmz787_> not much, just a protocol for optimization of electroporation... but I guess siRNA was used as the quantification metric
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12:56 < kanzure> "The fountain of youth by targeting senescent cells?" http://diyhpl.us/~bryan/papers2/longevity/The%20fountain%20of%20youth%20by%20targeting%20senescent%20cells%3f%20-%202017.pdf (2017)
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13:05 < kanzure> "In addition, two elegant constructs have been designed, INK-ATTAC and p16::3MR, to allow for drug-inducible suicide genes to selectively eliminate p16ink4a-positive senescence at any time [5,7]."
13:05 < kanzure> [5] Baker, D.J. et al. (2011) Clearance of p16Ink4a-positive senescent cells delays ageing-associated disorders. Nature 479, 232-236
13:05 < kanzure> [7] Demaria, M. et al. (2014) An essential role for senescent cells in optimal wound healing through secretion of PDGF-AA. Dev. Cell 31, 722-733
13:11 < kanzure> gonna have a bunch of combinatorial multiplexing problems with all the drug-activable switchtrigger gene stuff
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13:12 < kanzure> instead, we should design some sort of mRNA trigger system, where small molecule RNA gets accepted into most cells expressing a special receptor type
13:13 < kanzure> and the RNA could be specifically developed for delivery to the entire body.. somehow.
13:13 < kanzure> and somehow you would also have to make sure these aptamers are highly specific to certain epigenetic states or whatever
13:15 < kanzure> you would switch your body state by taking a big dose and wait a few days/weeks for whole body state to get flipped over, and then a few more weeks for expression results to be observable
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13:33 < kanzure> "A simple practice guide for dose conversion between animals and human" https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4804402/
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14:35 < kanzure> "A high resolution whole brain imaging using oblique light sheet tomography" http://biorxiv.org/content/early/2017/04/30/132423 https://twitter.com/biorxiv_neursci/status/858773039978336258
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15:14 < kanzure> andytoshi: https://en.wikipedia.org/wiki/Sleep_state_misperception
15:15 < kanzure> "most commonly used for people who mistakenly perceive their sleep as wakefulness"
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15:19 < kanzure> http://www.circadiansleepdisorders.org/
15:21 < kanzure> hm, not a helpful site.
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15:31 < darsie> http://science.orf.at/stories/2836518/
15:31 < darsie> Unsterblich dank Quantenbewusstsein?
15:31 < kanzure> "the rational hatter"
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15:46 < andytoshi> regarding sleep state misperception, the person you mentioned who thinks he needs very little sleep is, in fact awake ....as evidenced by his online activity
15:47 < andytoshi> it would be interesting if somebody thought they were awake, were actively posting on reddit, and yet were phisiologically asleep. that's like whales sleeping half their brains so they don't forget to breathe
15:48 < cluckj> I feel like reddit is replete with those posters...
15:55 < justanotheruser> do whales alternate which part of the brain is asleep each night?
15:56 < cluckj> they probably switch between lobes, like dolphins?
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19:15 < kanzure> blork
19:26 < kanzure> how to source high quality candidates for mutantpedia
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20:19 < gnusha_> https://secure.diyhpl.us/cgit/diyhpluswiki/commit/?id=e0c516b8 Bryan Bishop: add note about INK-ATTAC and p16::3MR >> http://diyhpl.us/diyhpluswiki/genetic-modifications/
20:25 < kanzure> "SLIT2-mediated ROBO2 signaling restricts kidney induction to a single site" http://www.cell.com/developmental-cell/fulltext/S1534-5807(04)00108-X
20:25 < kanzure> mutations in slit2 on 4p15.2 seem to cause supernumerary kidneys (extra kidneys) but unfortunately it's also axon guidance related
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22:28 < nmz787_> well my laser cutter is almost setup, I only need to take water and a USB hub to the garage now
22:28 < nmz787_> but not tonight
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--- Log closed Mon May 01 00:00:41 2017