--- Log opened Sun May 07 00:00:47 2017
00:18 < kanzure> .title https://www.youtube.com/watch?v=Y0Ani2aw6cM
00:18 < yoleaux> HGP-write: A Grand Challenge for Genome Engineering - YouTube
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00:31 < kanzure> .tw https://twitter.com/casa_tuthill/status/857273754283417600
00:31 < yoleaux> A short movie about my lab's research on fly walking and proprioception, animated by the amazing Sophie Tintori: https://vimeo.com/user9183457/flyproprioception?ref=tw-share ... https://pbs.twimg.com/media/C-WldJ_UQAABqKl.jpg (@casa_tuthill)
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01:33 < kanzure> .title https://cibolo.us/pipermail/open_electroporator/2017-May/000513.html
01:33 < yoleaux> [Open_electroporator] First test
01:33 < kanzure> https://cibolo.us/pipermail/open_electroporator/attachments/20170502/2c8eb1f8/attachment-0001.jpg
01:34 < kanzure> "Here is a scope shot of a pulse train firing off. Top trace is ADC divider volts, 0.1V with a 10X probe, so 1V/div. Bottom trace is the output HV with a 100X probe  5V * 100 = 500V/div."
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12:25 < kanzure> jrayhawk: which one of these are important? https://www.ebi.ac.uk/gwas/search?query=fasting
12:28 < kanzure> "STXBP5L and facial aging" http://www.jidonline.org/article/S0022-202X(15)36201-1/fulltext (rs322458)
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14:00 < kanzure> Stephan Zuchner, University of Miami Leonard M. Miller School of Medicine, "An In-Situ Digital Annotation System to Document and Safeguard GP-write Applications"
14:00 < kanzure> wat
14:01 < kanzure> Kenji Tsuge, Kobe University, "Long and precise genomic DNA construction using Bacillus subtilis"
14:02 < kanzure> Bogumil Karas, University of Western Ontario, "Development of a Pipeline for Precision Cloning and Effective Storage of Large Synthetic or Natural Human DNA Fragments"
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14:13 < emeraldgreen> >Bogumil Karas, University of Western Ontario, "Development of a Pipeline for Precision Cloning and Effective Storage of Large Synthetic or Natural Human DNA Fragments"
14:13 < emeraldgreen> Hmm, looks like this is going to mammal-scale chromosome synthesis
14:13 < emeraldgreen> I wonder if epigenetics will be a dealbreaker. I hope not.
14:14 < emeraldgreen> Yeast are fine with synthetic chromosomes, but yeast are simplest of eukaryotes
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14:17 < kanzure> yes they are doing genome synthesis
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14:40 < kanzure> esmerelda: yes it's re: human genome synthesis project
14:41 < kanzure> er.. emeraldgreen, rather.
14:45 < emeraldgreen> it'd be very cool & useful to have some software to model morphogenesis at the cell & cell-signaling level
14:47 < emeraldgreen> ... and have more general systems biology models
14:47 < kanzure> nah that all sounds hard, let's just replace biology with something simpler and more easily understood
14:48 < emeraldgreen> without these capabilities benefits of synthesizing a whole human genome are quite limited (not zero though - as with yeast, synthetic chromosomes are more clean - from transposons and other genetic trash - and more easily modified)
14:49 < emeraldgreen> >let's just replace bio
14:49 < emeraldgreen> I guess it's an ironic reference to over-optimistic futurists, kek
14:51 < kanzure> no, there's a lot of cruft in human biology
15:03 < kanzure> .wik xeno nucleic acid
15:03 < yoleaux> "Xeno nucleic acid (XNA) is a synthetic alternative to the natural nucleic acids DNA and RNA as information-storing biopolymers that differs in the sugar backbone. As of 2011, at least six types of synthetic sugars have been shown to form nucleic acid backbones that can store and retrieve genetic information." -- https://en.wikipedia.org/wiki/Xeno_nucleic_acid
15:03 < kanzure> "XNA exhibits a variety of structural chemical changes relative to its natural counterparts. Types of synthetic 'XNA' created so far include 1,5-anhydrohexitol nucleic acid (HNA) and cyclohexene nucleic acid (CeNA). Threose nucleic acid (TNA), glycol nucleic acid (GNA), locked nucleic acid (LNA), and peptide nucleic acid (PNA)"
15:03 < kanzure> i always thought that the X in XNA referred to a chemical name startin with x
15:03 < kanzure> *starting
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16:55 < emeraldgreen> kanzure XNA is interesting, reminds me about synthetic protocells
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19:45 < jrayhawk> kanzure: not seeing anything resembling results on that page; you're on your own
19:46 < jrayhawk> http://www.sens.org/research/extramural/scaling-up-glucosepane-research or i could just maintain high glutathione levels, or I could just not be diabetic or ketogenic and thus not have to worry about dicarbonyl production?
19:47 < jrayhawk> sens is so weird
19:49 < jrayhawk> TBH ketosis upregulates Nrf2, so I guess the methylglyoxal is already not an issue there
19:50 < jrayhawk> so much metabolic syndrome terminal pathology, so little regard for etiology
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--- Log closed Mon May 08 00:00:48 2017