--- Log opened Fri May 12 00:00:51 2017 00:04 -!- darsie [~darsie@84-113-55-42.cable.dynamic.surfer.at] has quit [Ping timeout: 240 seconds] 00:15 -!- Malvolio [~Malvolio@unaffiliated/malvolio] has quit [Quit: sleep] 00:17 -!- augur [~augur@noisebridge130.static.monkeybrains.net] has quit [Remote host closed the connection] 00:59 -!- Gurkenglas [~Gurkengla@dslb-188-103-222-233.188.103.pools.vodafone-ip.de] has joined ##hplusroadmap 01:02 -!- augur [~augur@2601:640:8001:4222:55b5:43ca:fac:b568] has joined ##hplusroadmap 01:22 -!- Proteus [~Proteus@unaffiliated/proteus] has quit [Read error: Connection reset by peer] 01:24 -!- Proteus [~Proteus@unaffiliated/proteus] has joined ##hplusroadmap 01:26 -!- Gurkenglas [~Gurkengla@dslb-188-103-222-233.188.103.pools.vodafone-ip.de] has quit [Ping timeout: 255 seconds] 01:29 -!- adlai [~adlai@unaffiliated/adlai] has quit [Read error: Connection reset by peer] 01:30 -!- adlai [~adlai@unaffiliated/adlai] has joined ##hplusroadmap 01:45 -!- emeraldgreen [~user@95-55-4-135.dynamic.avangarddsl.ru] has joined ##hplusroadmap 01:59 -!- sachy [~sachy@77.87.241.77] has joined ##hplusroadmap 02:20 -!- CheckDavid [uid14990@gateway/web/irccloud.com/x-klcjomxlmrlsftlg] has joined ##hplusroadmap 02:30 -!- TinKode [~TinKode@unaffiliated/tinkode] has joined ##hplusroadmap 02:57 -!- Proteus [~Proteus@unaffiliated/proteus] has quit [Write error: Connection reset by peer] 02:58 -!- Proteus [~Proteus@unaffiliated/proteus] has joined ##hplusroadmap 03:02 -!- augur [~augur@2601:640:8001:4222:55b5:43ca:fac:b568] has quit [Remote host closed the connection] 03:03 -!- augur [~augur@2601:640:8001:4222:55b5:43ca:fac:b568] has joined ##hplusroadmap 03:08 -!- augur [~augur@2601:640:8001:4222:55b5:43ca:fac:b568] has quit [Ping timeout: 246 seconds] 03:13 -!- emeraldgreen [~user@95-55-4-135.dynamic.avangarddsl.ru] has quit [Quit: Leaving.] 04:47 -!- darsie [~darsie@84-113-55-42.cable.dynamic.surfer.at] has joined ##hplusroadmap 04:48 < kanzure> justanotheruser: not much documentation, no, although there was a vimeo video of a talk i gave about meetlog 04:48 < kanzure> i also use jotmuch for tagging bookmarks on command line 06:12 -!- anthk_ [~ander@70.85-84-113.dynamic.clientes.euskaltel.es] has quit [Remote host closed the connection] 06:14 -!- TinKode [~TinKode@unaffiliated/tinkode] has quit [Ping timeout: 255 seconds] 06:16 -!- TinKode [~TinKode@unaffiliated/tinkode] has joined ##hplusroadmap 06:16 -!- TinKode [~TinKode@unaffiliated/tinkode] has quit [Client Quit] 06:16 -!- thesnark [~mike@unaffiliated/thesnark] has joined ##hplusroadmap 06:39 -!- poppingtonic [~brian@unaffiliated/poppingtonic] has joined ##hplusroadmap 06:39 -!- emeraldgreen [~user@ip-227-184-112-82.1lt.su] has joined ##hplusroadmap 06:39 -!- emeraldgreen [~user@ip-227-184-112-82.1lt.su] has left ##hplusroadmap [] 06:56 -!- mf1008 [~mf1008@unaffiliated/mf1008] has quit [Quit: Cave quid dicis, quando, et cui] 06:57 -!- mf1008 [~mf1008@unaffiliated/mf1008] has joined ##hplusroadmap 07:45 -!- Waterfall [~wat3rfall@hst-20-110.splius.lt] has joined ##hplusroadmap 07:45 -!- Waterfall [~wat3rfall@hst-20-110.splius.lt] has quit [Client Quit] 08:11 -!- drewbot [~cinch@54.147.248.113] has quit [Remote host closed the connection] 08:11 -!- drewbot [~cinch@107.20.31.234] has joined ##hplusroadmap 08:21 -!- Gurkenglas [~Gurkengla@dslb-188-103-222-233.188.103.pools.vodafone-ip.de] has joined ##hplusroadmap 08:36 -!- Malvolio [~Malvolio@unaffiliated/malvolio] has joined ##hplusroadmap 09:16 -!- poppingtonic [~brian@unaffiliated/poppingtonic] has quit [Ping timeout: 240 seconds] 09:37 -!- thesnark [~mike@unaffiliated/thesnark] has quit [Quit: "back later"] 10:12 -!- red-002 is now known as red-004 10:13 -!- red-004 is now known as red-002 10:14 -!- red-002 is now known as red-0003 10:15 -!- red-0003 is now known as red-00 10:17 -!- red-00 is now known as red-NaN 11:17 < justanotheruser> no results for meetlog seem relevant 11:17 < justanotheruser> is that sofwware? 11:18 < kanzure> it's my 5.5 megabyte plaintext yaml file of every conversation i've ever had 11:18 < kanzure> since 2009, at least. 11:18 < justanotheruser> oh 11:18 < justanotheruser> after a conversation you write it down? 11:18 < justanotheruser> I think we went over this 11:18 < kanzure> yes 11:18 < justanotheruser> You would know whether we did though 11:19 < kanzure> 2014-09-03 and 2017-01-04 11:20 < justanotheruser> thats a bit scary 11:25 -!- JenElizabeth [~Jen3@cpc76808-brmb10-2-0-cust571.1-3.cable.virginm.net] has joined ##hplusroadmap 12:28 -!- Gurkenglas [~Gurkengla@dslb-188-103-222-233.188.103.pools.vodafone-ip.de] has quit [Ping timeout: 255 seconds] 12:46 -!- red-NaN is now known as red-002 12:50 -!- Gurkenglas [~Gurkengla@dslb-188-103-222-233.188.103.pools.vodafone-ip.de] has joined ##hplusroadmap 13:03 -!- augur [~augur@74-95-6-5-SFBA.hfc.comcastbusiness.net] has joined ##hplusroadmap 13:41 -!- augur [~augur@74-95-6-5-SFBA.hfc.comcastbusiness.net] has quit [Remote host closed the connection] 13:52 -!- Douhet [~Douhet@unaffiliated/douhet] has quit [Ping timeout: 240 seconds] 13:53 -!- Douhet [~Douhet@unaffiliated/douhet] has joined ##hplusroadmap 14:12 -!- justan0theruser [~justanoth@unaffiliated/justanotheruser] has joined ##hplusroadmap 14:15 -!- justanotheruser [~justanoth@unaffiliated/justanotheruser] has quit [Ping timeout: 240 seconds] 14:30 -!- JenElizabeth [~Jen3@cpc76808-brmb10-2-0-cust571.1-3.cable.virginm.net] has quit [Ping timeout: 240 seconds] 14:30 -!- JenElizabeth [~Jen3@cpc76808-brmb10-2-0-cust571.1-3.cable.virginm.net] has joined ##hplusroadmap 14:52 -!- poppingtonic [~brian@unaffiliated/poppingtonic] has joined ##hplusroadmap 15:30 -!- JenElizabeth [~Jen3@cpc76808-brmb10-2-0-cust571.1-3.cable.virginm.net] has quit [Ping timeout: 260 seconds] 15:36 -!- JenElizabeth [~Jen3@cpc76808-brmb10-2-0-cust571.1-3.cable.virginm.net] has joined ##hplusroadmap 15:49 -!- poppingtonic [~brian@unaffiliated/poppingtonic] has quit [Ping timeout: 246 seconds] 16:00 -!- poppingtonic [~brian@unaffiliated/poppingtonic] has joined ##hplusroadmap 16:14 -!- JenElizabeth [~Jen3@cpc76808-brmb10-2-0-cust571.1-3.cable.virginm.net] has quit [Remote host closed the connection] 16:14 -!- JenElizabeth [~Jen3@cpc76808-brmb10-2-0-cust571.1-3.cable.virginm.net] has joined ##hplusroadmap 16:32 -!- darsie [~darsie@84-113-55-42.cable.dynamic.surfer.at] has quit [Ping timeout: 245 seconds] 16:44 -!- Douhet [~Douhet@unaffiliated/douhet] has quit [Read error: Connection reset by peer] 16:50 -!- poppingtonic [~brian@unaffiliated/poppingtonic] has quit [Ping timeout: 240 seconds] 16:59 -!- JenElizabeth [~Jen3@cpc76808-brmb10-2-0-cust571.1-3.cable.virginm.net] has quit [Remote host closed the connection] 16:59 -!- JenElizabeth [~Jen3@cpc76808-brmb10-2-0-cust571.1-3.cable.virginm.net] has joined ##hplusroadmap 17:02 -!- JenElizabeth [~Jen3@cpc76808-brmb10-2-0-cust571.1-3.cable.virginm.net] has quit [Remote host closed the connection] 17:03 -!- JenElizabeth [~Jen3@cpc76808-brmb10-2-0-cust571.1-3.cable.virginm.net] has joined ##hplusroadmap 17:04 < kanzure> blort. 17:25 -!- augur [~augur@noisebridge130.static.monkeybrains.net] has joined ##hplusroadmap 17:44 -!- jaboja [~jaboja@vps.jaboja.pl] has joined ##hplusroadmap 17:50 -!- JenElizabeth [~Jen3@cpc76808-brmb10-2-0-cust571.1-3.cable.virginm.net] has quit [Remote host closed the connection] 17:51 -!- JenElizabeth [~Jen3@cpc76808-brmb10-2-0-cust571.1-3.cable.virginm.net] has joined ##hplusroadmap 17:54 -!- strangewarp [~strangewa@c-76-25-206-3.hsd1.co.comcast.net] has joined ##hplusroadmap 18:01 -!- JenElizabeth [~Jen3@cpc76808-brmb10-2-0-cust571.1-3.cable.virginm.net] has quit [Remote host closed the connection] 18:02 -!- JenElizabeth [~Jen3@cpc76808-brmb10-2-0-cust571.1-3.cable.virginm.net] has joined ##hplusroadmap 18:09 -!- JenElizabeth [~Jen3@cpc76808-brmb10-2-0-cust571.1-3.cable.virginm.net] has quit [Remote host closed the connection] 18:10 -!- JenElizabeth [~Jen3@cpc76808-brmb10-2-0-cust571.1-3.cable.virginm.net] has joined ##hplusroadmap 18:10 -!- Jen [~Jen3@cpc76808-brmb10-2-0-cust571.1-3.cable.virginm.net] has joined ##hplusroadmap 18:14 -!- Jen [~Jen3@cpc76808-brmb10-2-0-cust571.1-3.cable.virginm.net] has quit [Remote host closed the connection] 18:14 -!- JenElizabeth [~Jen3@cpc76808-brmb10-2-0-cust571.1-3.cable.virginm.net] has quit [Ping timeout: 260 seconds] 18:15 -!- Jen [~Jen3@cpc76808-brmb10-2-0-cust571.1-3.cable.virginm.net] has joined ##hplusroadmap 18:31 -!- Jen [~Jen3@cpc76808-brmb10-2-0-cust571.1-3.cable.virginm.net] has quit [Remote host closed the connection] 18:32 -!- Jen [~Jen3@cpc76808-brmb10-2-0-cust571.1-3.cable.virginm.net] has joined ##hplusroadmap 18:41 -!- Gurkenglas [~Gurkengla@dslb-188-103-222-233.188.103.pools.vodafone-ip.de] has quit [Ping timeout: 240 seconds] 18:44 < kanzure> you guys are boring 18:44 -!- emeraldgreen [~user@95-55-4-135.dynamic.avangarddsl.ru] has joined ##hplusroadmap 18:48 -!- Jen [~Jen3@cpc76808-brmb10-2-0-cust571.1-3.cable.virginm.net] has quit [Remote host closed the connection] 18:48 -!- Jen [~Jen3@cpc76808-brmb10-2-0-cust571.1-3.cable.virginm.net] has joined ##hplusroadmap 18:55 -!- Jen [~Jen3@cpc76808-brmb10-2-0-cust571.1-3.cable.virginm.net] has quit [Remote host closed the connection] 18:56 -!- Jen [~Jen3@cpc76808-brmb10-2-0-cust571.1-3.cable.virginm.net] has joined ##hplusroadmap 19:01 -!- Jen [~Jen3@cpc76808-brmb10-2-0-cust571.1-3.cable.virginm.net] has quit [Remote host closed the connection] 19:02 -!- Jen [~Jen3@cpc76808-brmb10-2-0-cust571.1-3.cable.virginm.net] has joined ##hplusroadmap 19:03 -!- emeraldgreen [~user@95-55-4-135.dynamic.avangarddsl.ru] has left ##hplusroadmap [] 19:09 -!- strangewarp [~strangewa@c-76-25-206-3.hsd1.co.comcast.net] has quit [Read error: Connection reset by peer] 19:10 -!- strangewarp [~strangewa@c-76-25-206-3.hsd1.co.comcast.net] has joined ##hplusroadmap 19:17 -!- Jen [~Jen3@cpc76808-brmb10-2-0-cust571.1-3.cable.virginm.net] has quit [Ping timeout: 240 seconds] 19:25 -!- emeraldgreen [~user@95-55-4-135.dynamic.avangarddsl.ru] has joined ##hplusroadmap 19:28 -!- emeraldgreen [~user@95-55-4-135.dynamic.avangarddsl.ru] has quit [Remote host closed the connection] 19:31 -!- emeraldgreen [~user@95.55.4.135] has joined ##hplusroadmap 19:35 -!- strangewarp [~strangewa@c-76-25-206-3.hsd1.co.comcast.net] has quit [Read error: Connection reset by peer] 19:36 -!- strangewarp [~strangewa@c-76-25-206-3.hsd1.co.comcast.net] has joined ##hplusroadmap 19:42 -!- emeraldgreen1 [~user@95-55-125-150.dynamic.avangarddsl.ru] has joined ##hplusroadmap 19:43 -!- emeraldgreen [~user@95.55.4.135] has quit [Ping timeout: 240 seconds] 19:45 < kanzure> kent says instead of error-free oligo synthesis, we need large-scale messy DNA synthesis 19:45 -!- emeraldgreen1 [~user@95-55-125-150.dynamic.avangarddsl.ru] has quit [Remote host closed the connection] 19:45 < kanzure> better to push biology in a direction where writing things doesn't have to be perfect 19:47 < kanzure> so we could instead go find versions of these proteins that can tolerate up to 90% errors without impacting function 19:49 < kanzure> whatis stratos genomics 19:50 < streety> or redesign the translation machinery to be error tolerant 19:50 -!- JenElizabeth [~Jen3@cpc76808-brmb10-2-0-cust571.1-3.cable.virginm.net] has joined ##hplusroadmap 19:53 < kanzure> yeah maybe we could do n>5 length codons 19:54 < kanzure> or codons with error correction codes 19:57 < streety> it would depend somewhat on the types of errors you expect but might be simpler than optimizing every protein you ever need 19:59 < kanzure> well, you could do an error correction code that can tolerate certain kinds of errors and certain quantities of errors 20:00 < kanzure> however, increasing either of those dimensions will immensely complicate the tRNA machinery :( 20:03 < kanzure> perhaps a simpler project would start with only increasing the size of a codon 20:04 < streety> what sort of errors are likely? Is the issue insertion of incorrect bases, skipped bases, duplicated bases? Are they likely to be singular or would runs of multiple errors need to be handled? 20:04 < kanzure> there are many kinds of errors in phosphoramidite chemistry unfortunately 20:06 < kanzure> http://diyhpl.us/~bryan/papers2/DNA/Error%20analysis%20of%20chemically%20synthesized%20polynucleotides.pdf 20:14 < streety> mainly single bases getting skipped plus other weird errors, only 10 inserts sequenced though so relative rates somewhat uncertain 20:15 < streety> longer codons would not help with skipped bases as there would be a frameshift 20:16 < kanzure> well if you make the codons long enough you might have room to say fuzzy codon match only.. and then maybe spacers between codons. 20:17 < streety> error checking on the individual codons 20:17 < streety> I guess it could work, not easy though 20:25 < streety> I was thinking that if you attached a fluorescent protein to your protein of interest you could select against frameshift events and then remove the tag at the end. Presumably it's not just protein coding segments we need to consider though. All the non-coding sequences also need to be fault-tolerant 20:36 -!- Jen [~Jen3@cpc76808-brmb10-2-0-cust571.1-3.cable.virginm.net] has joined ##hplusroadmap 20:38 < kanzure> https://en.wikipedia.org/wiki/Error_detection_and_correction 20:39 -!- JenElizabeth [~Jen3@cpc76808-brmb10-2-0-cust571.1-3.cable.virginm.net] has quit [Ping timeout: 260 seconds] 20:59 -!- augur [~augur@noisebridge130.static.monkeybrains.net] has quit [Quit: Leaving...] 21:22 -!- g0d355__ [~lmao@104.131.75.159] has joined ##hplusroadmap 22:35 -!- jaboja [~jaboja@vps.jaboja.pl] has quit [Quit: Leaving] 22:58 -!- Jen [~Jen3@cpc76808-brmb10-2-0-cust571.1-3.cable.virginm.net] has quit [Read error: Connection reset by peer] 22:59 -!- Jen [~Jen3@cpc76808-brmb10-2-0-cust571.1-3.cable.virginm.net] has joined ##hplusroadmap 23:14 -!- sbodin [~sbodin@176.113.125.59] has joined ##hplusroadmap 23:32 -!- poppingtonic [~brian@unaffiliated/poppingtonic] has joined ##hplusroadmap 23:36 -!- poppingtonic [~brian@unaffiliated/poppingtonic] has quit [Ping timeout: 246 seconds] 23:39 -!- strangewarp_ [~strangewa@76.25.206.3] has joined ##hplusroadmap 23:42 -!- poppingtonic [~brian@unaffiliated/poppingtonic] has joined ##hplusroadmap 23:43 -!- strangewarp [~strangewa@c-76-25-206-3.hsd1.co.comcast.net] has quit [Ping timeout: 258 seconds] --- Log closed Sat May 13 00:00:52 2017