--- Log opened Sat May 20 00:00:59 2017
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05:07 < kanzure> adlai: sebastian cocioba has been playing with one on facebook.
05:14  * adlai gives facebook the ostritch treatment - if i ignore it for long enough, maybe other people will get a clue as well and we'll all deflate the advertising bubble together
05:14 < adlai> you're welcome to link to a specific (public) post, though
05:15 < kanzure> i don't really use it either, so i don't have such a link
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08:01 < kanzure> "current gene synthesis process has a typical error rate of 10^-2 to 10^-3, or 1-10 errors per kilo base-pairs (kbp) synthesized [7-10]." https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3933390/ (2011)
08:03 < kanzure> "Studies using the inkjet chip synthesis platformdetermined that the error rate can be reduced from 1 error in ~200 bases to 1 error in ~600 bases by using patterned silica features on a plastic chip [25]."
08:03 < kanzure> this is really not so high.
08:04 < kanzure> average protein of size 2 kbp will have three errors.. i guess that's dangerous.
08:04 < kanzure> "Polymerase-based cyclic assembly (PCA) methods allow the use of longer oligos and, as a result, the middle portion of the oligos are not subject to hybridization selection and tend to carry over more errors into assembled gene products [7, 26-28]."
08:09 < kanzure> but i also hear stuff like 1 error per 100 bp.
08:09 < kanzure> i wonder what counts as an error-- if you fail to insert 10 bp but you got the before and after correct, is that 1 error?
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12:14 < heath> random, crazy thought from me last night: couldn't investigation into https://arxiv.org/pdf/1006.4053.pdf lead to reduced error rates?
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12:33 < heath> i think it should, but that would be a difficult diybio research project
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15:13 < kanzure> "remember, DARPA's already vetted the polymerase -- they see no technical blocks to the prototype experiment"
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15:47 < heath> eh?
15:47 < kanzure> optically controlled polymerase stuff.
15:48 < heath> from which conversation was that though? :)
15:49 < kanzure> telepathy.
16:23 < kanzure> "we just have to add a single synthetic chromosome -- c24, that has all the code and switches and etc that we can add -- with elements that can be turned on or off at will"
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17:01 < kanzure> prototrophy funding announcement from a while back (i guess we missed this) https://www.eurekalert.org/pub_releases/2017-05/fkh-fgp050917.php
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20:04 < heath> .title https://en.wikipedia.org/wiki/IXS_Enterprise
20:04 < yoleaux> IXS Enterprise - Wikipedia
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--- Log closed Sun May 21 00:00:59 2017