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05:39 < kanzure> https://steemit.com/shadowbrokers/@theshadowbrokers/theshadowbrokers-monthly-dump-service-june-2017
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08:37 < nmz787_i> kanzure: how is that open hardware security module different from the TPMs that are already available? Are they trying to say the FPGA is more-open than a vendor-provided TPM?
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08:41 < kanzure> not clear to me yet, sorry
08:43 < kanzure> more music https://www.youtube.com/watch?v=b-c8imCPcR4
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10:29 < nmz787_i> "Many factors related to the design, construction materials, and operation of beamline and plasma systems can compromise the expected precision and cleanliness of the ion implantation process. In the last decade, the list of commonly used ions and target materials has widely expanded beyond the classic set of Si dopants and wafers, greatly increasing the complications for beam purity. Among the issues to be discussed are: (1) "mass 
10:29 < nmz787_i> charge exchange events resulting from collisions with residual gases, (2) transport of energetic and vapor phase metals and dopants, (3) wetting of device structures by contaminant-containing atmospheric water vapor during load lock pump down and following cryo-implants, (4) organic materials contamination from vacuum oils, o-rings and implantation of resist out-gassing products, (5) particle transport, adhesion and ion blocking effec
10:29 < nmz787_i> and effects in nano-scale IC devices will be discussed for each contamination issue."
10:30 < nmz787_i> https://www.researchgate.net/publication/307980998_Ion_Beam_Purity_and_Wafer_Contamination_An_IIT16_school_book_chapter
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10:38 < nmz787_i> hmm, so seems like as long as any contamination in the gallium vaporize, the beam will just have more 'chromatic' aberration
10:38 < nmz787_i> since those ions will likely have different masses, and will thus be affected differently by all the electrstatic lenses
10:39 < chris_99> would i be right in thinking the gallium is liquid in the FIB?
10:39 < nmz787_i> so probably lowering the resolution, but also making the cutting tip (tooling tip) in milling terms a different shape
10:40 < nmz787_i> chris_99: I think it is solid at room temp, and sitting in a coil which is able to be powered to act as a resistive heater, which can warm up the gallium
10:40 < chris_99> yeah i think it melts at 29C
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10:40 < nmz787_i> then that heater circuit is biased by kilovolts relative to the sample, to draw off ions
10:41 < chris_99> gotcha
10:42 < nmz787_i> these metals are literally graded by number of nines... the xN grading, where x is the number of nines
10:43 < nmz787_i> so amazon's 99.99% is 4N, sigma's 99.99999 would be 7N
10:43 < chris_99> heh interesting
10:43 < chris_99> do you know what type you have
10:43 < nmz787_i> or maybe it was 99.99995% which idk would be either 6N or 6.5N or 7N
10:43 < nmz787_i> no, was thinking of asking the seller, and/or a mailing list
10:44 < nmz787_i> but the seller is on that mailing list :P
10:44 < chris_99> cool, a mailing list for the specific FIB?
10:44 < chris_99> or generic one for FIBs?
10:44 < nmz787_i> and they're also the importer and either regional or whole country licensed servicer for the manufacturer
10:44 < nmz787_i> microscopy.com
10:45 < nmz787_i> it is mostly non-optical stuff
10:45 < chris_99> ah neat
10:45 < nmz787_i> err, non-photonic beams
10:46 < nmz787_i> *primary beams
10:46 < nmz787_i> since photoemission can happen with impinging electrons or ions
10:46 < chris_99> ah yeah, like x-ray emission or something?
10:46 < nmz787_i> and some use optical amplification for final presentation to the user
10:46 < nmz787_i> yeah
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11:34 < kanzure> nmz787_i: what about imaging
11:34 < nmz787_i> the FIB can do it
11:35 < nmz787_i> it is just going to wear down the surface with each scan
11:35 < nmz787_i> destructive imaging
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11:43 < kanzure> "Digital-to-biological converter for on-demand production of biologics" https://www.nature.com/nbt/journal/vaop/ncurrent/full/nbt.3859.html
11:43 < kanzure> "Manufacturing processes for biological molecules in the research laboratory have failed to keep pace with the rapid advances in automization and parellelization1, 2, 3. We report the development of a digital-to-biological converter for fully automated, versatile and demand-based production of functional biologics starting from DNA sequence information. Specifically, DNA templates, RNA molecul...
11:43 < kanzure> ...es, proteins and viral particles were produced in an automated fashion from digitally transmitted DNA sequences without human intervention."
11:48 < nmz787_i> https://bioautomation.com/products/mermade-192r
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11:49 < nmz787_i> "Upon detection of the oligonucleotide file (Supplementary Table 1), the DBC system controller transferred the file to the MerMade 192R oligonucleotide synthesizer (Online Methods, component 1 in Supplementary Figs. 1,2 and Supplementary Video 1). Once synthesis was complete, the oligonucleotide-containing columns were moved by the lower crane arm (Supplementary Fig. 1, component 5) to a vacuum nest. An ammonium hydroxide-methylamin
11:49 < nmz787_i> groups"....
11:50 < nmz787_i> http://www.nature.com/nbt/journal/vaop/ncurrent/fig_tab/nbt.3859_SF2.html
11:50 < nmz787_i> "Supplementary Figure 2: Route map of synthesis of a biological in DBC."
11:51 < nmz787_i> "The script 'Oligonucleotide Designer' (executable by WordPerfect), that was used for generating oligonucleotides from DNA sequence is available at https://github.com/kentboles/DBC and also in the Supplementary Code."
11:51 < nmz787_i> "The DBC system controller (Hudson Robotics) software is comprised of an interface written in Visual Basic that allows the user to define run characteristics such as the name of the run, oligonucleotide synthesis script and the cycling conditions for gene assembly."
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12:12 < nmz787_i> huh, Rigol has a new office in the next city over from me
12:13 < nmz787_i> "As more and more customers join the ranks of the RIGOL customer base we have maxed out the capacity of our current warehouse.  This is a great problem to have!"
12:25 < kanzure> sounds like they have an interface with their mermade 192r oligonucleotide synthesizer
12:32 < nmz787_i> yup
12:32 < nmz787_i> the picture is only 1 lab bench jammed top and bottom with stuff
12:39 < kanzure> hmm but it sounds like they have a separate visual basic tool for part of the process (they claimed no human intervention)
12:47 < nmz787_i> hmm, very comprehensive: https://www.hindawi.com/journals/jnt/2014/170415/
12:47 < nmz787_i> .title
12:47 < yoleaux> nmz787_i: Sorry, that doesn't appear to be an HTML page.
12:48 < kanzure> i have no idea what folder to place that in
12:49 < nmz787_i> wow, it has everything from history, calculations, applications like milling, patterning, nanopores, microfluidics, optical gratings, magnetic processing, self-directed assembly, plasmonics...
12:51 < kanzure> you should dust off your svg test image file
12:51 < kanzure> http://diyhpl.us/~bryan/papers2/diybio/mccorkle_tomkins-tinch_microchannels.svg
12:54 < nmz787_i> yeah not a bad idea
12:55 < kanzure> we should make a TODO list for projects to try out
12:56 < kanzure> for example, the programmable superhydrophobic surfaces using different gratings
12:56 < kanzure> er.. i mean, different gradients of parallel lines of varying widths and distances from each other.
12:58 < nmz787_i> mm, yeah, that would be a good list
12:58 < nmz787_i> there are some optical grating things I'd like to try
12:58 < nmz787_i> diffractive optics I guess you might call them
12:58 < nmz787_i> this guy seems like he might be cool: http://www.jcnabity.com/overview.htm
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12:59 < nmz787_i> he has his retirement plan for concerned customers " for anyone who might worry about retirement or catastrophic events, please let the following reassure you. In either of these cases, one of the following actions will take place"
13:00 < nmz787_i> either sell to another SEM company, sell or run jointly to a current smart customer... or place all into public domain
13:01 < kanzure> yeah optical grating patterns + piezo material should be on the list somewhere, lots of fun ultrasound things can be done if you pattern the pillars just right
13:03 < nmz787_i> hmm
13:04 < nmz787_i> I was just reading about some diamond nanopillar earlier, that when illuminated briefly, produced a short burst of electrons from its tip... was speculating x-ray testing could be shrunk to size of a pen with this as an electron beam source
13:06 < nmz787_i> not counting time to swap parts, recalibrate, etc... which could be many hours, idk yet... base cost of $3700 for 1000 hours is still $3.7 / hour of beam time... which doesn't sound too expensive for small volumes of things
13:07 < nmz787_i> or if you're using the beam to make a master of something you can replicate with other means (photomask, imprint mold, etc)
13:07 < nmz787_i> certainly lowering the cost to $66 for a refill would be way better....
13:08 < kanzure> it is unclear to me what kind of multi-layer mems stuff can be made without requiring chemical etch steps. it looks like tension-based micromechanical structures (beams, rods, etc.) can be done pretty easily.
13:08 < nmz787_i> I as wondering if the impurities would aggregate for somereason due to gravity and the gallium being liquid... but I guess probably not
13:08 < nmz787_i> kanzure: also this machine has carbon deposition... but I am not sure how strong it is
13:09 < nmz787_i> and seems like I might be able to hack in platinum, though it seems every add-on or refill for this is at least $4-8k :O
13:09 < nmz787_i> but seems like platinum would be used much less than primary beam... just for active electrodes and such
13:11 < nmz787_i> I guess Tungsten was piloted by Seiko, but they have the patent for any metallocarbonyl  (I think)... and then FEI came in and started selling Platinum but suit was never brought against them
13:11 < nmz787_i> or so I was told
13:12 < nmz787_i> apparently something I read in the docs folder said the carbon was too resistive for jumping circuit logic
13:12 < nmz787_i> but I guess in the meantime, since I have no sputter coater, I might be able to use it to coat an entire field-of-view in carbon so I can image it
13:13 < nmz787_i> (unless the sample itself is sufficiently conductive)
13:13 < nmz787_i> then it's fine on its own
13:22 < nmz787_i> "Liquid Metal Ion Sources, used for Liquid Metal Ion Sources, used for droplets in high velocity, low mass droplets in high velocity, low mass spacecraft engines ( spacecraft engines (Krohn, TRW, 1973)"
13:23 < nmz787_i> that replication was a powerpoint copy-paste error
13:23 < nmz787_i> http://www.physics.utah.edu/Phys5739/lecture/feiBasicFIB.pdf
13:29 < nmz787_i> "(methylcyclopentadienyl) trimethyl platinum )"
13:30 < nmz787_i> says for that at least, it is user refillable in a fume hood
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14:21 < kanzure> nmz787_i: is your SEM working?
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15:06 < nmz787_i> kanzure: not yet, I have new oil and an almost-complete fan-based cooler for the diffusion pump (it just needs the controls and pump and power supply mounted in the frame I made for it all)
15:09 < darsie> How long till (cryo?)preservation for mind uploading is ready?
15:10 < darsie> Can it be done today?
15:20 < nmz787_i> darsie: with enough money, maybe by tomorrow
15:26 < darsie> So any dislocations due to ice crystals can be computationally reversed?
15:27 < darsie> Or ... sufficiently?
15:31 < kanzure> darsie: synaptic barcoding could get a lot of the data without using cryopreservation
15:31 < kanzure> darsie: http://diyhpl.us/~bryan/papers2/neuro/Using%20high-throughput%20barcode%20sequencing%20to%20efficiently%20map%20connectomes%20-%20v3%20-%202017.pdf
15:31 < kanzure> http://diyhpl.us/~bryan/papers2/neuro/High-throughput%20mapping%20of%20single%20neuron%20projections%20by%20sequencing%20of%20barcoded%20RNA%20-%202016.pdf
15:32 < kanzure> in the mean time, aldehyde preservation seems to do okay things: http://diyhpl.us/wiki/transcripts/verifiable-brain-preservation/
15:33 < darsie> I need something that preserves me till I can get scanned.
15:40 < darsie> Does barcode sequencing read connection weights? I think that's a crucial part of the mind.
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15:56 < kanzure> darsie: you can read connection weights with FISSEQ
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16:06 < nmz787_i> I'd guess a custom dual/triple-beam cryo-chilled vacuum system should work.... with a big enough sample chamber for a head to fit in and keep cold. FIB the top off like a diamond edge microtome (or a nano belt sander), image with SEM/STEM and do tomography... maybe
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17:07 < nmz787_i> we had a party while you were gone kanzure...
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20:02 < kanzure> oh
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--- Log closed Fri Jun 02 00:00:10 2017