--- Log opened Wed Aug 30 00:00:04 2017
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00:09 < BingoBoingo> !~ticker --market all
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00:13 < nmz787> juri_: just saw Keoni over the weekend in Oakland
00:15 < nmz787> kanzure: doesn't seem like it is up yet, contact John if you want him to send another board to someone (so far I seem to be the only person who has bio and electronics know how and debug equipment)
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00:16 < nmz787> kanzure: also he probably deserves some more $, would you consider some sort of donation matching or something?
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06:26 < juri_> nmz787: he's talking about some sort of free synthesis on the synthbio mailing list.
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07:31 < kanzure> nmz787: lol donations. no. if he needs money then he should just ask me for it.
07:31 < kanzure> juri_: which mailing list is that?
07:34 < kanzure> has anyone looked into coffee resistance reset
07:35 < juri_> 'network of independent biospaces.'
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08:52 < kanzure> .title https://news.ycombinator.com/item?id=15132833
08:52 < yoleaux> Two-thirds of Americans approve of editing human DNA to treat disease | Hacker News
08:52 < kanzure> https://www.theverge.com/2017/8/30/16198594/crispr-gene-editing-survey-public-opinion
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10:17 < kanzure> this tech transfer office displays all their 'tech' on the web, apparently: http://tlo.mit.edu/technologies/method-rna-guided-endonuclease-based-dna-assembly
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10:37 < kanzure> "mung bean nuclease" alright yashgaroth where do you find this stuff
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11:39 < kanzure> Tsubashi: howdy
11:40 < Tsubashi> how's everything going kanzure?
11:41 < kanzure> going alright. what brings you here?
11:50 < Tsubashi> oh shoot
11:50 < Tsubashi> I see it changed my nick over on this server too
11:50 < Tsubashi> I've been in here before as Asterion
11:51 < kanzure> ah yes i am bad at reading. it says as much.
11:51 < Tsubashi> still figuring out irc
11:52 < Tsubashi> not sure how to have a nick here and a different nick on rizon
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12:01 < kanzure> "If no one minds I'd like to make a couple of points (though I haven't yet read the articles). NF-kB is a transcription factor responsible for the production of numerous target genes concerned with inflammation - genes that produce inflammation. Inflammation is associated with oxidative stress because high levels of ROS is produced as part of the signaling process that receipt of an ...
12:01 < kanzure> ...inflammatory ligand like IL-1beta, TNF (one doesn't use the 'alpha' suffix any more), IL-8 etc.) because ROS production is part of the signaling process. NF-kB is also the primary complex required for cellular senescence - as it not only is responsible for SASP (senescence-associated secretory phenomenon) - the production of exactly those sorts of cytokines that cause the activation and ...
12:01 < kanzure> ...nuclear translocation of NF-kB (and many other things as well). Also, simply raising the level of ROS production (and specifically, hydrogen peroxide production) is enough to activate NF-kB (detach it from Ikb-alpha that keeps it in the cytoplasm).  So really the cause of atherosclerosis is the invasion of oxidized-lipid ingesting (before it was believed that this applied to cholesterol ...
12:01 < kanzure> ...alone) macrophages. It is clear that after macrophage ingest these oxidized fats they become the 'foam cells' that cause atherosclerosis by producing all sorts of cytokines and growth factors, and it seems clear to me that these foam cells are nothing more than the senescent form of macrophage. Also, remember NAMPT is in mammals the enzyme that controls the rate-limiting step in the synthesis ...
12:01 < kanzure> ...of glutathione - a primary resource for reducing (with the aid of glutaredoxins and peroxiredoxins (two of the most common proteins in cells and present in all cells - at least peroxiredoxin is as the glutathione (GSH) dependent system originated later in evolution).  Glutathione controls the concentration of ROS - so that's where the stories are connected.  Nrf2 is also concerned with ...
12:01 < kanzure> ...glutathione production and reduction (as well as repair enzymes). - Harold"
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12:15 < Tsubashi> who is harold?
12:16 < kanzure> dunno. just something from my inbox.
12:16 < Tsubashi> thanks for sharing, I'm unfamiliar with some of the processes mentioned. clearly have some homework to do on atherosclerosis
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12:25 < Tsubashi> seems to be missing some previous discussion about NAMPT
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12:31 < Tsubashi> where is this excerpt from, if you don't me asking, kanzure?
12:31 < Tsubashi> or do you just receive random snippets of convo
12:32 < kanzure> i receive random conversations yea. this one was from "grg".
12:33 < kanzure> ("gerontology research group")
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13:08 < nmz787> justanotheruser: yep, 500 1.8kb fragments free and openMTA licensed each month for the next 4 months, then a 10000 fragments order
13:08 < nmz787> kanzure: well we've already just done donations to him
13:08 < nmz787> kanzure: I guess donations with expectations
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13:11 < nmz787> juri_:  yep, 500 1.8kb fragments free and openMTA licensed each month for the next 4 months, then a 10000 fragments order
13:11 < nmz787> sorry justanotheruser
13:12 < nmz787> anyone in here know chinese well enough to help me through registering and ordering from taobao?
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13:23 < kanzure> "Providing genes encoding pathways on designer chromosomes, such as all genes required to make a prototrophic human cell"
13:24 < kanzure> "GP-write and HGP-write would proceed in  phases  according  to  a  roadmap (Appendix 1) to evaluate feasibility and value, just as HGP-read, ENCODE and the  Synthetic  Yeast  Project also  began  with  pilot  projects  (Appendix  2) focusing on a fraction of  the genome: typically ~ 1% but not limited  to  this amount. Intense  technology  development  would  also  occur  during  this  3-5 ...
13:24 < kanzure> ...year  period  to  facilitate  the  other  99%.   During this  3-5  year  period,  there would be ample time to debate whether a specific full HGP-write project (i.e. whole genome) design was scientifically, economically and ethically justified, or whether whole genome activities would be better slanted toward a model system such as the mouse. "
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15:53 < kanzure> blort
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18:30 < nmz787> heh "economically"
18:30 < nmz787> like, making it so needs to be part of the roadmap
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20:43 < abetusk> https://www.youtube.com/watch?v=Rk_TbNyZdDg
20:43 < abetusk> .title
20:43 < yoleaux> Transparent digital microfluidic devices from etched ITO foil - YouTube
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22:11 < ybit> https://news.ycombinator.com/item?id=15134670 - Bitcoin's Academic Pedigree
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23:57 -!- mrdata [~mrdata@unaffiliated/mrdata] has quit [Read error: Connection reset by peer]
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--- Log closed Thu Aug 31 00:00:04 2017