--- Log opened Fri Sep 22 00:00:25 2017 00:05 -!- esmerelda [~mabel@71-212-29-109.tukw.qwest.net] has joined ##hplusroadmap 00:05 -!- esmerelda [~mabel@71-212-29-109.tukw.qwest.net] has quit [Changing host] 00:05 -!- esmerelda [~mabel@unaffiliated/jacco] has joined ##hplusroadmap 00:15 -!- Aurelius [~cpopell@50.35.79.117] has quit [Ping timeout: 240 seconds] 00:29 -!- Gurkenglas [~Gurkengla@dslb-178-000-177-083.178.000.pools.vodafone-ip.de] has joined ##hplusroadmap 00:36 -!- aeiousom1thing [~aeiousome@gateway/vpn/privateinternetaccess/aeiousomething] has quit [Ping timeout: 252 seconds] 00:36 -!- Nikopol_ [~NSohru@37.48.65.48] has joined ##hplusroadmap 00:40 -!- NikopolSohru [~NSohru@37.48.65.48] has quit [Ping timeout: 260 seconds] 00:41 -!- Aurelius [~cpopell@50.35.79.117] has joined ##hplusroadmap 00:54 -!- RebelCoder [~Yuriy@95.143.115.254] has quit [Remote host closed the connection] 00:58 -!- darsie [~username@84-113-55-42.cable.dynamic.surfer.at] has joined ##hplusroadmap 01:03 -!- Malvolio [~Malvolio@unaffiliated/malvolio] has quit [Read error: Connection reset by peer] 01:05 -!- pepesza [~pepesza@185.83.218.228] has quit [Ping timeout: 240 seconds] 01:11 -!- pepesza [~pepesza@185.83.218.228] has joined ##hplusroadmap 01:25 -!- Gurkenglas [~Gurkengla@dslb-178-000-177-083.178.000.pools.vodafone-ip.de] has quit [Ping timeout: 255 seconds] 01:39 -!- traumschule [~traumschu@185.104.184.179] has joined ##hplusroadmap 01:43 -!- CRM114 [~urchin@unaffiliated/urchin] has joined ##hplusroadmap 01:53 -!- poppingtonic [~brian@unaffiliated/poppingtonic] has joined ##hplusroadmap 01:53 -!- poppingtonic [~brian@unaffiliated/poppingtonic] has quit [Read error: Connection reset by peer] 01:54 -!- poppingtonic [~brian@unaffiliated/poppingtonic] has joined ##hplusroadmap 01:54 -!- poppingtonic [~brian@unaffiliated/poppingtonic] has quit [Client Quit] 01:55 -!- poppingtonic [~brian@unaffiliated/poppingtonic] has joined ##hplusroadmap 01:57 -!- aeiousomething [~aeiousome@gateway/vpn/privateinternetaccess/aeiousomething] has joined ##hplusroadmap 02:15 -!- aeiousomething [~aeiousome@gateway/vpn/privateinternetaccess/aeiousomething] has quit [Ping timeout: 240 seconds] 02:36 -!- poppingtonic [~brian@unaffiliated/poppingtonic] has quit [Read error: Connection reset by peer] 02:36 -!- poppingtonic [~brian@unaffiliated/poppingtonic] has joined ##hplusroadmap 02:40 -!- c0rw1n_ [~c0rw1n@206.41-244-81.adsl-dyn.isp.belgacom.be] has joined ##hplusroadmap 02:50 -!- aeiousomething [~aeiousome@gateway/vpn/privateinternetaccess/aeiousomething] has joined ##hplusroadmap 02:53 -!- dustinm- [~dustinm@68.ip-149-56-14.net] has quit [Quit: Leaving] 02:54 -!- dustinm [~dustinm@68.ip-149-56-14.net] has joined ##hplusroadmap 02:57 -!- jtimon [~quassel@199.31.134.37.dynamic.jazztel.es] has joined ##hplusroadmap 03:00 -!- augur [~augur@noisebridge130.static.monkeybrains.net] has quit [Remote host closed the connection] 03:30 -!- poppingtonic [~brian@unaffiliated/poppingtonic] has quit [Ping timeout: 255 seconds] 03:41 -!- augur [~augur@198-27-215-123.static.sonic.net] has joined ##hplusroadmap 04:32 -!- jaboja [~jaboja@jaboja.pl] has joined ##hplusroadmap 04:32 -!- augur [~augur@198-27-215-123.static.sonic.net] has quit [Remote host closed the connection] 04:37 -!- c0rw1n_ [~c0rw1n@206.41-244-81.adsl-dyn.isp.belgacom.be] has quit [Quit: Leaving] 04:39 -!- c0rw1n_ [~c0rw1n@206.41-244-81.adsl-dyn.isp.belgacom.be] has joined ##hplusroadmap 05:05 -!- jaboja [~jaboja@jaboja.pl] has quit [Ping timeout: 248 seconds] 05:07 < kanzure> "Computing exponentially faster: implementing a non-deterministic universal Turing machine using DNA" https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5378132/ 05:09 -!- jaboja [~jaboja@jaboja.pl] has joined ##hplusroadmap 06:16 -!- jaboja [~jaboja@jaboja.pl] has quit [Ping timeout: 260 seconds] 06:17 -!- poppingtonic [~brian@unaffiliated/poppingtonic] has joined ##hplusroadmap 06:21 -!- poppingtonic [~brian@unaffiliated/poppingtonic] has quit [Ping timeout: 240 seconds] 06:22 -!- poppingtonic [~brian@unaffiliated/poppingtonic] has joined ##hplusroadmap 06:35 -!- Nikopol_ [~NSohru@37.48.65.48] has quit [Ping timeout: 255 seconds] 06:36 -!- poppingtonic [~brian@unaffiliated/poppingtonic] has quit [Ping timeout: 248 seconds] 06:40 -!- NikopolSohru [~NSohru@37.48.65.48] has joined ##hplusroadmap 06:50 -!- jaboja [~jaboja@jaboja.pl] has joined ##hplusroadmap 07:02 -!- justanotheruser [~justanoth@unaffiliated/justanotheruser] has quit [Ping timeout: 264 seconds] 07:09 -!- jaboja [~jaboja@jaboja.pl] has quit [Ping timeout: 264 seconds] 07:11 < kanzure> researchgate spam: "Mohamed Helmy, an author that cited you, commented on an article: " 07:11 < kanzure> this is really extreme surveillance, whoever implemented this must be mindless 07:13 < kanzure> .title https://ipscell.com/2017/09/1st-knockout-human-embryos-made-crispr-take-pub/ 07:13 < yoleaux> 1st Knockout Human Embryos Made with CRISPR: My Take on the Pub - The Niche 07:18 < adlai> from the previous link: "Advocates of the many-worlds interpretation of quantum mechanics argue that QUTMs work through exploitation of the hypothesized parallel universes [8,13,49]. Intriguingly, if the multiverse were an NUTM this would explain the profligacy of worlds." 07:19 < kanzure> advocates of many-worlds shall be hanged and/or dismembered 07:20 -!- jtimon [~quassel@199.31.134.37.dynamic.jazztel.es] has quit [Ping timeout: 240 seconds] 07:23 < cluckj> (temporary?) livestream of biohacker summit: https://www.pscp.tv/w/1OyKArgLnmDGb 07:25 < juul> cluckj: nice 07:25 < cluckj> did you make it there okay? 07:26 < cluckj> it's not my stream, but I am watching it :) 07:26 < cluckj> crap. 07:26 < juul> yeah i'm here 07:26 < juul> what? 07:26 < cluckj> the stream is gone now 07:27 < juul> it's back. the person before requested not to be recorded 07:29 < cluckj> https://www.pscp.tv/w/1MnGnmdwrYjKO 07:29 < cluckj> ah okay 07:29 < cluckj> someone before ellen? 07:29 < juul> before the person before ellen 07:29 < cluckj> lol 07:30 < kanzure> thanks 07:30 < juul> btw looks like there is a pro recording happening but maybe not live-streaming 07:30 < kanzure> ugh 07:31 < kanzure> please record audio so that i can come back and type later, i'm busy doing things over here :| 07:31 < juul> i'll go ask 07:31 < cluckj> I am so bummed about being under a fucking rock for two years with my dissertation burnout 07:31 < kanzure> blame it on the kid, cluckj 07:31 < cluckj> thanks, I'd like to see a better quality version 07:32 < cluckj> kanzure, it was dissertation burnout + kid + moving to philly that was keeping me too busy to really pay attention to diybio :\ 07:32 < kanzure> (hint: never blame the kid for your poor choices) 07:32 < cluckj> ;) 07:32 < cluckj> I'm not blaming him :P 07:34 < cluckj> who didn't want to be recorded/broadcasted? if you shouldn't answer, that is okay 07:36 < juul> i don't remember 07:37 < cluckj> k 07:37 < juul> here is th eoffifical link 07:37 < juul> https://www.facebook.com/1407362332713384/videos/1407653942684223/ 07:37 < cluckj> thanks so much! 07:38 < cluckj> no audioooo 07:38 < juul> ok will go check what's going on 07:39 < cluckj> :) 07:40 < kanzure> you gonna type transcripts, juul? 07:40 < juul> ok they are fixing it 07:41 < cluckj> awesome, thanks so much again 07:41 < juul> i can't type that fast, sorry 07:47 < cluckj> kanzure that FB video link is now showing the entirety of the recording 07:48 < cluckj> almost 2 hours... 07:50 < juul> we're on break 07:50 < juul> they said the audio is working now 07:50 < juul> cluckj: can you check? 07:50 < juul> oh i guess you can't 07:51 < cluckj> I can't check, they went off live 07:51 < cluckj> now I'm trying to download the video... 07:54 < cluckj> official youtube link, too: https://www.youtube.com/watch?v=1V155vBRWEs 08:01 < cluckj> aw man, the audio cuts out like 10 minutes in 08:02 < cluckj> that was *only* on the FB livestream -- the youtube is okay 08:08 < cluckj> live is back on youtube 08:14 < kanzure> type notes not statuses 08:14 < kanzure> win the war 08:15 < cluckj> it's all being recorded, I don't need no stinkin' notes!! 08:17 < kanzure> that's how you lose the war on time. 500 people view a 1 hour video: 500 hours. 500 people read some text: 83 hours. 08:18 < cluckj> there's additional material in the video that is difficult to transcribe :P 08:18 -!- Gurkenglas [~Gurkengla@dslb-178-000-177-083.178.000.pools.vodafone-ip.de] has joined ##hplusroadmap 08:18 < cluckj> what I mean is I can take notes later 08:18 < kanzure> later --> now you spend 2x the time on this 08:19 < kanzure> you guys are not very good timelords 08:19 < cluckj> only 1.5x if I watch it at double speed 08:20 < cluckj> trust me, I'm a doctor 08:35 -!- jaboja [~jaboja@jaboja.pl] has joined ##hplusroadmap 08:36 -!- Gurkenglas [~Gurkengla@dslb-178-000-177-083.178.000.pools.vodafone-ip.de] has quit [Ping timeout: 240 seconds] 08:38 < kanzure> .tw https://twitter.com/_markel___/status/910567298083250182 08:38 < yoleaux> We did it: Intel ME 11.x arbitrary code execution, #BHEU https://www.blackhat.com/eu-17/briefings/schedule/#how-to-hack-a-turned-off-computer-or-running-unsigned-code-in-intel-management-engine-8668 (@_markel___) 08:42 -!- Malvolio [~Malvolio@unaffiliated/malvolio] has joined ##hplusroadmap 08:43 < cluckj> damn this conference is really cool 08:48 < kanzure> .tw https://twitter.com/Noahpinion/status/909585825804849152 08:48 < yoleaux> Most of the great scientific discoveries in history were made before peer review was common: http://marginalrevolution.com/marginalrevolution/2017/09/peer-review-younger-think.html (@Noahpinion) 08:52 -!- Gurkenglas [~Gurkengla@dslb-178-000-177-083.178.000.pools.vodafone-ip.de] has joined ##hplusroadmap 09:14 -!- Gurkenglas [~Gurkengla@dslb-178-000-177-083.178.000.pools.vodafone-ip.de] has quit [Read error: Connection reset by peer] 09:22 < kanzure> http://grognor.blogspot.se/2017/03/a-history-of-weird-sun-twitter.html 09:40 -!- Gurkenglas [~Gurkengla@dslb-178-000-177-083.178.000.pools.vodafone-ip.de] has joined ##hplusroadmap 10:04 < maaku> adlai: pilot wave theory is a simpler interpretation of quantum mechanics that doesn't result in bat-shit-crazy many worlds 10:11 < adlai> from my stupidly superficial understanding of QM, the door to "many worlds" is opened by the uncertainty principle; but because observations happen within a single world, occam's razor just shaves away all the others 10:13 < kanzure> maaku: thanks. 10:23 < pasky> kanzure: ...what exactly am I trying to read there?! 10:23 < pasky> "In November 2012, Instance Of Class appeared. This is the same month during which I lost my virginity, my father got married, and I got forcibly sent to a psychiatric hospital for the first time." 10:24 < kanzure> just some weird internet shit, man. 10:25 < cluckj> this current talk is freaking awesome 10:25 -!- streety_ [~streety@156.40.254.24] has joined ##hplusroadmap 10:26 < kanzure> what is it about 10:27 < cluckj> Mboa Thomas on global contexts for biohacking 10:29 < kanzure> their youtube links keep changing https://www.youtube.com/watch?v=YDjg6OOPQm0 10:29 < cluckj> definitely rewind to the beginning if you can 10:30 < pasky> kanzure: those twitters are actually pretty good though :) 10:31 < kanzure> heh 10:32 -!- streety_ [~streety@156.40.254.24] has quit [Ping timeout: 240 seconds] 10:36 < kanzure> https://twitter.com/hashtag/globalbiosummit 10:37 < kanzure> .tw https://twitter.com/mnlimas/status/911279177935986688 10:37 < yoleaux> A New Enzymatic Approach to #DNASynthesis Could be the Next Breakthrough in #synbio https://synbiobeta.com/new-enzymatic-approach-dna-synthesis-next-breakthrough-synthetic-biology/ @molassemblies #SBBSF17 #DNA https://pbs.twimg.com/media/DKUfDTOW0AAWzn5.jpg (@mnlimas) 10:38 -!- streety_ [~streety@156.40.254.24] has joined ##hplusroadmap 10:38 < kanzure> .tw https://twitter.com/crispr_de/status/910594078403973120 10:38 < yoleaux> DNA editing in human embryos reveals role of fertility "master gene" https://www.theguardian.com/science/2017/sep/20/dna-editing-in-human-embryos-reveals-role-of-fertility-master-gene (@crispr_de) 10:38 < kanzure> "Enhanced proofreading governs CRISPR–Cas9 targeting accuracy" http://www.nature.com/nature/journal/vaap/ncurrent/full/nature24268.html https://twitter.com/shsternberg/status/910616247649288192 10:39 < kanzure> .tw https://twitter.com/edyong209/status/910115091307220992 10:39 < yoleaux> Delete one gene, and suddenly these butterflies get dazzling blue iridescence https://www.theatlantic.com/science/archive/2017/09/the-genes-that-paint-butterflies/540159/ https://pbs.twimg.com/media/DKFg0CfX0AMdppu.jpg (@edyong209) 10:40 -!- streety_ [~streety@156.40.254.24] has quit [Client Quit] 10:41 < kanzure> http://sf2017.synbiobeta.com/sessions/step-step-guide-genetically-modifying-crispr/ 10:41 < kanzure> "This workshop will guide you through the steps for how anyone could potentially modify themselves with CRISPR technology and how we are making that possible. It will cover things such as where to obtain materials, key decision points, and provide a handy guide for you to take home with you!" 10:42 < kanzure> .tw https://twitter.com/GeneMods/status/908453473569705985 10:42 < yoleaux> Impressive. IndieBio companies now have raised a combined $100 million, and have a combined $500 million market cap. #indiebio #demoday (@GeneMods) 10:43 < kanzure> .tw https://twitter.com/EricTopol/status/907639739204771840 10:43 < yoleaux> Something's wrong when it's cheaper to get a kidney transplant than engineered T cells https://www.nytimes.com/2017/09/11/health/cost-gene-therapy-drugs.html?_r=1 2017 data @FortuneMagazine https://pbs.twimg.com/media/DJiU-KcU8AAUtkF.jpg (@EricTopol) 10:46 < kanzure> .tw https://twitter.com/pknoepfler/status/907342253391097856 10:46 < yoleaux> USA Today opinion piece. 'Just chill about #CRISPR & designer babies, folks', say authors, to paraphrase https://www.usatoday.com/story/opinion/2017/09/08/human-embryo-editing-wont-lead-designer-babies-berezow-locwin-column/581489001/ (@pknoepfler) 10:48 < kanzure> .tw https://twitter.com/CRISPR_News/status/906328749079093248 10:48 < yoleaux> In world-first, Japanese scientists use CRISPR to change flower color http://bit.ly/2eTBF0s via @GeneticLiteracy (@CRISPR_News) 10:48 < kanzure> .tw https://twitter.com/synbio_papers/status/906464602073964545 10:48 < yoleaux> Strategies for editing virulent staphylococcal phages using CRISPR-Cas10. http://dlvr.it/PlqrnC https://pbs.twimg.com/media/DJRowBCUEAEJNvt.jpg (@synbio_papers) 10:48 < kanzure> https://www.ncbi.nlm.nih.gov/pubmed/28885820 10:49 < kanzure> .tw https://twitter.com/antonioregalado/status/905859731759222784 10:49 < yoleaux> More than half of the 28 authors of Venter's face prediction paper don't work at Human Longevity anymore. https://www.technologyreview.com/s/608813/does-your-genome-predict-your-face-not-quite-yet https://pbs.twimg.com/media/DJJCkURUwAAMnxv.jpg (@antonioregalado) 10:50 < kanzure> .tw https://twitter.com/tanner_jnl_club/status/905287238510104576 10:50 < yoleaux> Julian: RNA-aptamers-in-droplers (RAPID) high-throughput screening for secretory phenotypes https://www.nature.com/articles/s41467-017-00425-7 (@tanner_jnl_club) 10:51 < JayDugger> Back to your code review! 10:53 < kanzure> i'm not petertodd. 11:09 -!- c0rw1n_ [~c0rw1n@206.41-244-81.adsl-dyn.isp.belgacom.be] has quit [Ping timeout: 248 seconds] 11:17 -!- jaboja [~jaboja@jaboja.pl] has quit [Ping timeout: 260 seconds] 11:18 < nmz787> cluckj: I had a dream last night or the night before that I was in Philly and thinking whether it was an interesting place to move/settle... my dream featured hills/small mountain ranges... more like L.A. so it was a weird dream (I've only driven through Philly like twice when I was a kid) 11:18 < cluckj> lol 11:19 < cluckj> geographically it's...pretty flat 11:19 < cluckj> but it's a nice place 11:21 < nmz787> hmm, so that facebook link seems to not be live anymore? 11:21 < cluckj> kanzure, cell-free biotech 11:21 < cluckj> nmz787, https://www.youtube.com/watch?v=yJmY1rc82kE 11:22 < nmz787> kewl 11:25 < kanzure> in ivtro protein things? 11:25 < nmz787> "ming. Wallstreet is here. #Bitcoin https://t.co/kB7AtI6z0W https://t.co/cOx9S98nmZ 11:25 < nmz787> 15:38 < kanzure> man i have such a hard time writing 11:25 < nmz787> wtf 11:25 < nmz787> that isn't waht I copied at all 11:25 < cluckj> yes 11:25 < nmz787> "[Microscopy] Decomissioned TEM / Siemens Elmiskop 102" (available in Chicago) 11:25 < nmz787> 70s era 11:25 < nmz787> (I am not interested realistically) 11:26 < kanzure> it's true that writing annoys me in a really deep untouchably weird way 11:26 < nmz787> BCI 4 all! 11:27 < cluckj> yeeep 11:27 < cluckj> all the presenters so far have been fantastic 11:31 < cluckj> "how to build your own bioprinter" 11:34 -!- Gurkenglas [~Gurkengla@dslb-178-000-177-083.178.000.pools.vodafone-ip.de] has quit [Ping timeout: 248 seconds] 11:36 -!- streety_ [~streety@156.40.254.24] has joined ##hplusroadmap 11:39 -!- jaboja [~jaboja@jaboja.pl] has joined ##hplusroadmap 12:07 -!- jtimon [~quassel@199.31.134.37.dynamic.jazztel.es] has joined ##hplusroadmap 12:08 -!- streety_ [~streety@156.40.254.24] has quit [Quit: ZNC 1.6.5 - http://znc.in] 12:17 -!- mindsForge [~nak@174-26-38-214.phnx.qwest.net] has joined ##hplusroadmap 12:36 < kanzure> mindsForge: welcome. 12:36 < kanzure> mindsForge: here's some text, http://gnusha.org/logs/2017-09-22.log 12:37 < kanzure> or other stuff http://gnusha.org/logs/2017-09-21.log 12:38 < mindsForge> thanks, I was actually just scrolling through the largest log file 12:39 < mindsForge> a good idea to check out recent ones 12:40 < cluckj> haha, major shade being thrown at for-profit publishing 12:41 < kanzure> well throw some shade back about scihub (where's our copy?) 12:41 < cluckj> I don't think this is a sci-hub project? 12:41 < cluckj> this is pubpun 12:41 < cluckj> err pubpub 12:42 < kanzure> oh the 4chan of academic peer review shitposting? 12:42 < kanzure> no wait, that's pubpeer 12:42 < kanzure> sorry 12:43 < cluckj> doesn't look like it 12:43 < cluckj> oh 12:43 < cluckj> he wants people to tell him what's wrong with it so it can be fixed 12:44 < kanzure> someone dropped a link to pubpub in here a while back, but it didn't make enough sense https://www.pubpub.org/pub/setting-the-scene-for-freedom-of-form-an-evolution-revolution---introduction 12:45 < cluckj> if you check out the talk, it seems a little more developed now? 12:54 < cluckj> ugh, citizen health innovator stuff 12:55 < cluckj> the work is cool, but that phrase gives me the creeps 13:21 -!- mindsForge [~nak@174-26-38-214.phnx.qwest.net] has quit [Ping timeout: 240 seconds] 13:24 -!- mindsForge [~nak@174-26-38-214.phnx.qwest.net] has joined ##hplusroadmap 13:46 -!- mindsForge [~nak@174-26-38-214.phnx.qwest.net] has quit [Ping timeout: 248 seconds] 13:49 -!- mindsForge [~nak@174-26-65-3.phnx.qwest.net] has joined ##hplusroadmap 14:00 < kanzure> .tw https://twitter.com/AaronJDy/status/911234327282843648 14:00 < yoleaux> 3 challenges for 🐷➡️🤒 organ transplants: immune rejection, physiological incompatibility, and 🐷➡️🤒 microbes http://science.sciencemag.org/content/357/6357/1238 (@AaronJDy) 14:00 < kanzure> "Advances in organ transplant from pigs" http://science.sciencemag.org/content/357/6357/1238 14:01 -!- Gurkenglas [~Gurkengla@dslb-178-000-177-083.178.000.pools.vodafone-ip.de] has joined ##hplusroadmap 14:02 < kanzure> "Axonal synapse sorting in medial entorhinal cortex" http://www.nature.com/nature/journal/vaop/ncurrent/full/nature24005.html 14:02 < kanzure> "Here we used dense reconstructions from three-dimensional electron microscopy in rats to study the synaptic organization of local presynaptic axons in layer 2 of the medial entorhinal cortex, the site of grid-like spatial representations. We observe path-length-dependent axonal synapse sorting, such that axons of excitatory neurons sequentially target inhibitory neurons followed by ... 14:02 < kanzure> ...excitatory neurons. Connectivity analysis revealed a cellular feedforward inhibition circuit involving wide, myelinated inhibitory axons and dendritic synapse clustering. Simulations show that this high-precision circuit can control the propagation of synchronized activity in the medial entorhinal cortex, which is known for temporally precise discharges." 14:16 -!- CRM114 [~urchin@unaffiliated/urchin] has quit [Read error: Connection reset by peer] 14:43 -!- traumschule [~traumschu@185.104.184.179] has quit [Ping timeout: 246 seconds] 15:13 -!- NikopolSohru [~NSohru@37.48.65.48] has quit [Ping timeout: 255 seconds] 15:28 -!- HEx1 [~HEx@hexwab.plus.com] has joined ##hplusroadmap 15:46 < kanzure> the "no true scotsman fallacy" fallacy: i had never claimed anything about the scots... 15:49 -!- HEx1 [~HEx@hexwab.plus.com] has quit [Remote host closed the connection] 15:49 -!- HEx1 [~HEx@hexwab.plus.com] has joined ##hplusroadmap 15:51 < nmz787> there are some decent still-ish shots from the livestreams... i can ID at least a few known players 15:51 < nmz787> some of the faces I only recognize but don't rememeber their name 15:52 < kanzure> no doxxing 15:52 < nmz787> hahaha 15:53 < nmz787> "could daisy drive help make New Zealand predator-free?" -- I doubt that talk mentioned elimating criminal/ill-intentioned humans 15:59 -!- HEx1 [~HEx@hexwab.plus.com] has quit [Ping timeout: 252 seconds] 16:27 -!- augur [~augur@noisebridge130.static.monkeybrains.net] has joined ##hplusroadmap 16:31 < nmz787> https://www.change.org/p/chip-and-cheese-lovers-bring-cheddar-beer-back-to-kettle-chips 16:31 < nmz787> plz sign my petition... I need tasty potato chips 16:32 < nmz787> sidenote -- I feel like this might be a supply chain issue, as another very-tasty cheddar cheese chip brand also discontinued that flavor... leaving only the sub-par cheese chips on the market 16:32 < cluckj> you're in PGH? 16:32 < nmz787> well, I was 16:32 < nmz787> now that is my online cover story 16:32 < cluckj> oh, I was going to suggest Herr's 16:33 -!- HEx1 [~HEx@hexwab.plus.com] has joined ##hplusroadmap 16:34 < nmz787> yeah they are OK 16:34 < nmz787> grandma Utz's are where it's at... they use lard for frying 16:35 < nmz787> but I believe they all lack the same cheesiness of either Kettle Chips Cheddar Beer, or Tim's Cheddar Chips 16:36 < nmz787> I actually ordered some of the Tim's version, idk what to call them, "New-Old-Stock" chips from Amazon.com 16:36 < nmz787> i.e. they were pre-cancellation, just sitting in some warehouse/dudes house 16:36 < cluckj> herr's makes a lard-fried chip that is better & less greasy than utz's 16:36 < nmz787> they tasted fine though, for being old stored chips 16:36 < nmz787> oO 16:37 < nmz787> time to make Herrs mine 16:37 < cluckj> they're pretty rare 16:37 < nmz787> wow, they sell online :) 16:38 < nmz787> "*** Please note that bags shipped to the West coast my incur popping due to high altitude in transportation. Please order accordingly! Most of our snack items have a shelf life of at least 6 to 7 weeks so please order your snacks accordingly." 16:39 < cluckj> take care of your snacks 16:39 -!- CheckDavid [uid14990@gateway/web/irccloud.com/x-ejxrxeuvswfkmlzo] has joined ##hplusroadmap 16:44 -!- jaboja [~jaboja@jaboja.pl] has quit [Remote host closed the connection] 16:47 -!- mrdata [~mrdata@unaffiliated/mrdata] has joined ##hplusroadmap 16:49 -!- justanotheruser [~justanoth@unaffiliated/justanotheruser] has joined ##hplusroadmap 17:12 -!- augur [~augur@noisebridge130.static.monkeybrains.net] has quit [Remote host closed the connection] 17:33 -!- justanotheruser [~justanoth@unaffiliated/justanotheruser] has quit [Ping timeout: 252 seconds] 17:33 -!- augur [~augur@noisebridge130.static.monkeybrains.net] has joined ##hplusroadmap 17:37 -!- aeiousomething [~aeiousome@gateway/vpn/privateinternetaccess/aeiousomething] has quit [Ping timeout: 246 seconds] 17:44 < kanzure> "dna origami masks for semiconductor photolithography" 17:57 -!- poppingtonic [~brian@unaffiliated/poppingtonic] has joined ##hplusroadmap 18:04 -!- poppingtonic [~brian@unaffiliated/poppingtonic] has quit [Ping timeout: 248 seconds] 18:05 -!- poppingtonic [~brian@unaffiliated/poppingtonic] has joined ##hplusroadmap 18:06 < mindsForge> kanzure: have a link for that one? 18:07 -!- Gurkenglas_ [~Gurkengla@dslb-178-012-146-214.178.012.pools.vodafone-ip.de] has joined ##hplusroadmap 18:07 < kanzure> it's from src.org not sure if this is a public document 18:09 < kanzure> would probably be better to make protein sheets with holes, instead of 2d dna origami tiles. 18:10 -!- Gurkenglas [~Gurkengla@dslb-178-000-177-083.178.000.pools.vodafone-ip.de] has quit [Ping timeout: 240 seconds] 18:14 -!- augur [~augur@noisebridge130.static.monkeybrains.net] has quit [Remote host closed the connection] 18:15 -!- augur [~augur@noisebridge130.static.monkeybrains.net] has joined ##hplusroadmap 18:16 < mindsForge> yeah, I just cant imagine the logistics using DNA directly 18:16 -!- Gurkenglas__ [~Gurkengla@dslb-178-008-024-060.178.008.pools.vodafone-ip.de] has joined ##hplusroadmap 18:16 < mindsForge> neat that people are experimenting w/that idea though 18:18 -!- poppingtonic [~brian@unaffiliated/poppingtonic] has quit [Ping timeout: 240 seconds] 18:19 -!- Gurkenglas_ [~Gurkengla@dslb-178-012-146-214.178.012.pools.vodafone-ip.de] has quit [Ping timeout: 240 seconds] 18:21 < kanzure> "Efficient targeted DNA methylation with chimeric dCas9–Dnmt3a–Dnmt3L methyltransferase" https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5389507/ 18:22 < kanzure> "A programmable Cas9-serine recombinase fusion protein that operates on DNA sequences in mammalian cells" http://evolve.harvard.edu/123-RecCas9.pdf 18:22 < kanzure> "Epigenetic editing of Ascl1 gene in neural stem cells by optogenetics" http://www.nature.com/articles/srep42047 18:23 < kanzure> "Peaks of targeted methylation were observed around 25 bp upstream and 40 bp downstream of the PAM site, while 20–30 bp of the binding site itself are protected against methylation. Potent methylation is dependent on the multimerization of Dnmt3a/Dnmt3L complexes on the DNA. Furthermore, the introduced methylation causes transcriptional repression of the targeted genes. These new ... 18:23 < kanzure> ...programmable epigenetic editors allow unprecedented control of the DNA methylation status in cells and will lead to further advances in the understanding of epigenetic signaling." 18:23 < kanzure> (or data storage *cough*) 18:27 < kanzure> "... using a single guide RNA, dCas9-SunTag-DNMT3A is able to methylate a 4.5-kb genomic region" https://genomebiology.biomedcentral.com/articles/10.1186/s13059-017-1306-z 18:34 < kanzure> "Nonenzymatic labeling of 5-hydroxymethylcytosine in nanopore sequencing" http://onlinelibrary.wiley.com/doi/10.1002/cbic.201300342/full 18:36 -!- poppingtonic [~brian@unaffiliated/poppingtonic] has joined ##hplusroadmap 18:37 -!- mrdata [~mrdata@unaffiliated/mrdata] has quit [Read error: Connection reset by peer] 18:41 < kanzure> "Real-time single-molecule electronic DNA sequencing by synthesis using polymer-tagged nucleotides on a nanopore array" http://www.pnas.org/content/113/19/5233.short 18:42 < kanzure> "Design and characterization of a nanopore-coupled polymerase for single-molecule DNA sequencing by synthesis on an electrode array" http://www.pnas.org/content/113/44/E6749.short 18:43 < kanzure> "When an incoming complementary-tagged nucleotide forms a tight ternary complex with the primer/template and polymerase, the tag enters the pore, and the current blockade level is measured. The levels displayed by the four nucleotides tagged with four different polymers captured in the nanopore in such ternary complexes were clearly distinguishable and sequence-specific, enabling continuous ... 18:43 < kanzure> ...sequence determination during the polymerase reaction." 18:43 < kanzure> uh... 18:44 -!- poppingtonic [~brian@unaffiliated/poppingtonic] has quit [Remote host closed the connection] 18:55 < kanzure> i wonder what the physical setup looks like... or if it matters; there should be nucleotides flying around everywhere in the liquid medium, why wouldn't you be detecting those instead of only the incorporation events? 18:55 -!- augur [~augur@noisebridge130.static.monkeybrains.net] has quit [Remote host closed the connection] 18:57 -!- augur [~augur@noisebridge130.static.monkeybrains.net] has joined ##hplusroadmap 18:59 -!- poppingtonic [~brian@unaffiliated/poppingtonic] has joined ##hplusroadmap 19:02 -!- augur [~augur@noisebridge130.static.monkeybrains.net] has quit [Ping timeout: 252 seconds] 19:07 < gnusha> https://secure.diyhpl.us/cgit/diyhpluswiki/commit/?id=e523eb38 Bryan Bishop: more methyltransferase papers >> http://diyhpl.us/diyhpluswiki/gene-editing/ 19:14 -!- poppingtonic [~brian@unaffiliated/poppingtonic] has quit [Ping timeout: 240 seconds] 19:18 -!- CheckDavid [uid14990@gateway/web/irccloud.com/x-ejxrxeuvswfkmlzo] has quit [Quit: Connection closed for inactivity] 19:21 -!- delinquentme_ [~delinquen@108-235-112-153.lightspeed.sntcca.sbcglobal.net] has quit [Quit: Leaving] 19:27 -!- augur [~augur@noisebridge130.static.monkeybrains.net] has joined ##hplusroadmap 19:43 -!- darsie [~username@84-113-55-42.cable.dynamic.surfer.at] has quit [Ping timeout: 252 seconds] 20:03 < kanzure> musics https://www.youtube.com/watch?v=mJkaGXFLO7U 20:16 -!- Cory [~Cory@unaffiliated/cory] has quit [Ping timeout: 248 seconds] 20:20 -!- Darius [~quassel@66-215-89-229.dhcp.psdn.ca.charter.com] has joined ##hplusroadmap 21:50 -!- jtimon [~quassel@199.31.134.37.dynamic.jazztel.es] has quit [Ping timeout: 246 seconds] 21:59 -!- Aurelius [~cpopell@50.35.79.117] has quit [Ping timeout: 240 seconds] 22:00 -!- Gurkenglas_ [~Gurkengla@dslb-188-103-079-072.188.103.pools.vodafone-ip.de] has joined ##hplusroadmap 22:03 -!- Gurkenglas__ [~Gurkengla@dslb-178-008-024-060.178.008.pools.vodafone-ip.de] has quit [Ping timeout: 248 seconds] 22:07 -!- jtimon [~quassel@199.31.134.37.dynamic.jazztel.es] has joined ##hplusroadmap 22:12 -!- sandeepkr [~sandeepkr@ec2-52-29-251-54.eu-central-1.compute.amazonaws.com] has quit [Ping timeout: 248 seconds] 22:14 -!- sandeepkr [~sandeepkr@ec2-52-29-251-54.eu-central-1.compute.amazonaws.com] has joined ##hplusroadmap 22:21 -!- hehelleshin [~talinck@cpe-174-97-113-184.cinci.res.rr.com] has joined ##hplusroadmap 22:25 -!- Guest57324 [~talinck@cpe-174-97-113-184.cinci.res.rr.com] has quit [Ping timeout: 246 seconds] 22:27 -!- Gurkenglas_ [~Gurkengla@dslb-188-103-079-072.188.103.pools.vodafone-ip.de] has quit [Ping timeout: 252 seconds] 22:33 -!- mrdata [~mrdata@unaffiliated/mrdata] has joined ##hplusroadmap 22:45 -!- Darius [~quassel@66-215-89-229.dhcp.psdn.ca.charter.com] has quit [Remote host closed the connection] 22:57 -!- aeiousomething [~aeiousome@183.82.170.54] has joined ##hplusroadmap 23:06 -!- mindsForge [~nak@174-26-65-3.phnx.qwest.net] has left ##hplusroadmap [] 23:23 -!- preview [~quassel@2407:7000:842d:4000::3] has joined ##hplusroadmap 23:30 -!- augur [~augur@noisebridge130.static.monkeybrains.net] has quit [Quit: Leaving...] 23:51 -!- justanotheruser [~justanoth@unaffiliated/justanotheruser] has joined ##hplusroadmap --- Log closed Sat Sep 23 00:00:11 2017