--- Log opened Mon Oct 09 00:00:41 2017 00:12 -!- poppingtonic [~brian@unaffiliated/poppingtonic] has quit [Ping timeout: 240 seconds] 00:23 -!- Coming [~Coming@55.92-220-69.customer.lyse.net] has joined ##hplusroadmap 00:33 -!- poppingtonic [~brian@unaffiliated/poppingtonic] has joined ##hplusroadmap 01:04 < nmz787> dx.doi.org/10.1021/nl103873a 01:04 < nmz787> .title 01:04 < yoleaux> nmz787: Sorry, that doesn't appear to be an HTML page. 01:04 < nmz787> DNA Tunneling Detector Embedded in a Nanopore 01:04 < nmz787> so, the (3) atomic-layer HVM nanopore array paper didn't use the sensing electrode in their paper :/ 01:05 < nmz787> and the paper ^ has much uglier nanpores and thicknesses 01:05 < nmz787> on the other hand, it was done with a FIB 01:06 < nmz787> which means possibly, just maybe, I could get it done myself 01:06 < nmz787> although I'm not sure about the silicon nitride 01:09 < nmz787> not sure the benefits of that over silicon dioxide, for an insulator 01:10 < nmz787> https://www.nature.com/nnano/journal/v5/n4/full/nnano.2010.42.html 01:10 < nmz787> .title 01:10 < yoleaux> Access : Identifying single nucleotides by tunnelling current : Nature Nanotechnology 01:11 < nmz787> so, this is basically what an STM does... except with a layer electrode, instead of a tip electrode 01:14 < nmz787> https://dberard.com/home-built-stm/electronics/ 01:17 < nmz787> "I didn’t notice any difference in image quality with the breadboarded electronics, but that’s because it depends more on the quality of the tips and vibration. The PCB I designed has lower noise on the scanner outputs but I can’t say I ever noticed a difference in the images." 01:17 < nmz787> useful for SEM/FIB design: "In this case, the HV amplifier is the piezo driver. If you use my design, it generates the following signals which have a range of +/- 10V: Z+X, Z-X, Z+Y, Z-Y. What you’d then have to do is amplify those 4 signals to a +/- 150V range (or whatever voltage range is suitable for your scanner). You could do this using two PA443DF dual op-amps for example." 01:18 < nmz787> i'm thinking for driving coils/plates for beam deflection 01:19 -!- poppingtonic [~brian@unaffiliated/poppingtonic] has quit [Ping timeout: 240 seconds] 01:27 < nmz787> .wik break junction 01:27 < yoleaux> "A break junction is an electronic device which consists of two metal wires separated by a very thin gap, on the order of the inter-atomic spacing (less than a nanometer). This can be done by physically pulling the wires apart or through chemical etching or electromigration." — https://en.wikipedia.org/wiki/Break_junction 01:31 -!- Gurkenglas [~Gurkengla@dslb-094-223-135-191.094.223.pools.vodafone-ip.de] has joined ##hplusroadmap 01:33 -!- poppingtonic [~brian@unaffiliated/poppingtonic] has joined ##hplusroadmap 01:38 -!- aeiousomething [~aeiousome@183.82.170.54] has joined ##hplusroadmap 01:39 -!- aeiousom1thing [~aeiousome@gateway/vpn/privateinternetaccess/aeiousomething] has joined ##hplusroadmap 01:43 -!- aeiousomething [~aeiousome@183.82.170.54] has quit [Ping timeout: 258 seconds] 01:49 -!- CRM114 [~urchin@unaffiliated/urchin] has joined ##hplusroadmap 01:54 -!- Gurkenglas [~Gurkengla@dslb-094-223-135-191.094.223.pools.vodafone-ip.de] has quit [Ping timeout: 240 seconds] 02:31 -!- c0rw1n_ [~c0rw1n@cpc109847-bagu17-2-0-cust223.1-3.cable.virginm.net] has quit [Ping timeout: 255 seconds] 03:01 -!- helleshin [~talinck@cpe-174-97-113-184.cinci.res.rr.com] has joined ##hplusroadmap 03:03 -!- hehelleshin [~talinck@cpe-174-97-113-184.cinci.res.rr.com] has quit [Ping timeout: 240 seconds] 05:45 -!- cluckj [~cluckj@static-173-59-27-112.phlapa.ftas.verizon.net] has quit [Quit: Leaving] 06:13 -!- Coming [~Coming@55.92-220-69.customer.lyse.net] has quit [Quit: Leaving] 06:25 < JayDugger> nmz787, really want to deal with a glorified CRT? 06:25 < JayDugger> Have you got better options? 06:35 -!- helleshin [~talinck@cpe-174-97-113-184.cinci.res.rr.com] has quit [Ping timeout: 255 seconds] 06:52 -!- Gurkenglas [~Gurkengla@dslb-094-223-135-191.094.223.pools.vodafone-ip.de] has joined ##hplusroadmap 07:30 -!- jaboja [~jaboja@jaboja.pl] has joined ##hplusroadmap 07:51 -!- poppingtonic [~brian@unaffiliated/poppingtonic] has quit [Quit: Leaving.] 07:56 < kanzure> .tw https://twitter.com/GeneMods/status/915620389195255808 07:56 < yoleaux> Wow. $35 billion in new lab equipment purchased every year. Wonder what the avg/median cost/instrument is, and price ∆ over time. #sbbsf17 (@GeneMods) 07:56 < kanzure> .tw https://twitter.com/kanzure/status/917195980063281152 07:56 < yoleaux> @GeneMods if the equipment was 10x cheaper, would we get 10x more science? (@kanzure, in reply to tw:915620389195255808) 07:56 < kanzure> .tw https://twitter.com/GeneMods/status/917383911709306880 07:56 < yoleaux> @kanzure I’d wager there’s some threshold where the return on democratization of biotech expertise and equipment is even higher than 1:1 (@GeneMods, in reply to tw:917195980063281152) 08:33 -!- Gurkenglas [~Gurkengla@dslb-094-223-135-191.094.223.pools.vodafone-ip.de] has quit [Ping timeout: 255 seconds] 08:33 -!- jaboja [~jaboja@jaboja.pl] has quit [Ping timeout: 255 seconds] 08:59 -!- Gurkenglas [~Gurkengla@dslb-094-223-135-191.094.223.pools.vodafone-ip.de] has joined ##hplusroadmap 09:34 < nmz787> JayDugger: I've got two CRTs in the room here with me now, along with a SEM that is basically an inverted TV... and another basically-inverted TV in the garage 09:35 < nmz787> JayDugger: I guess what were you referring to? the SEM/FIB design comment? 09:38 -!- jaboja [~jaboja@jaboja.pl] has joined ##hplusroadmap 10:00 -!- jaboja [~jaboja@jaboja.pl] has quit [Ping timeout: 240 seconds] 10:13 < JayDugger> nmz787, yup. 10:13 < JayDugger> And that answered my rhetorical question. 10:32 < kanzure> "crackpot version of arxiv.org" http://vixra.org/ 10:34 < kanzure> doesn't seem too bad http://vixra.org/bioch/ 11:07 -!- jaboja [~jaboja@jaboja.pl] has joined ##hplusroadmap 11:16 -!- c0rw1n_ [~c0rw1n@cpc109847-bagu17-2-0-cust223.1-3.cable.virginm.net] has joined ##hplusroadmap 11:23 -!- Aurelius [~cpopell@50.35.79.117] has joined ##hplusroadmap 12:00 < JayDugger> Isn't that where conspiracy theorists post their papers? 12:00 < JayDugger> Paul La Violette, for example? 12:00 < kanzure> it's not clear to me if all categories are for crackpots 12:00 < kanzure> i'm sure there's junk on here somewhere but perhaps this site was subjected to some misinformation/slander campaigns 12:01 < JayDugger> Fair enough. 12:13 -!- CRM114 [~urchin@unaffiliated/urchin] has quit [Remote host closed the connection] 12:18 -!- c0rw1n_ [~c0rw1n@cpc109847-bagu17-2-0-cust223.1-3.cable.virginm.net] has quit [Ping timeout: 240 seconds] 12:20 -!- aeiousom1thing [~aeiousome@gateway/vpn/privateinternetaccess/aeiousomething] has quit [Ping timeout: 248 seconds] 12:21 -!- jaboja [~jaboja@jaboja.pl] has quit [Ping timeout: 246 seconds] 13:03 -!- Betawolf [~matthew@xn--bta-yla.net] has joined ##hplusroadmap 13:06 -!- darsie [~username@84-114-73-160.cable.dynamic.surfer.at] has quit [Ping timeout: 240 seconds] 13:21 -!- y0no [y0no@2001:bc8:212d:201:ff01::a] has quit [Quit: Bye] 13:32 -!- y0no [y0no@2001:bc8:212d:201:ff01::a] has joined ##hplusroadmap 13:36 -!- y0no [y0no@2001:bc8:212d:201:ff01::a] has quit [Ping timeout: 258 seconds] 13:37 -!- y0no [y0no@2001:bc8:212d:201:ff01::a] has joined ##hplusroadmap 13:45 < kanzure> .tw https://twitter.com/BosLabMA/status/917309791978115072 13:45 < yoleaux> It's alive!!! Bioluminescent E. coli born on the benchtops of @BosLabMA! https://video.twimg.com/tweet_video/DLrwYTvW4AAkaiX.mp4 (@BosLabMA) 13:46 < kanzure> http://blogs.plos.org/synbio/2017/10/05/when-will-crispr-get-a-nobel-prize/ 13:47 < kanzure> .tw https://twitter.com/stephenfloor/status/917138083090358272 13:47 < yoleaux> Major problem in science that many journals reap the rewards of popular or controversial papers but no consequences if they’re wrong. https://twitter.com/mendel_random/status/917115714544324609 (@stephenfloor) 13:47 -!- Betawolf [~matthew@xn--bta-yla.net] has left ##hplusroadmap [] 13:49 < kanzure> "Anti-doping agency to ban all gene editing in sport from 2018" https://www.newscientist.com/article/2149768-anti-doping-agency-to-ban-all-gene-editing-in-sport-from-2018/ 13:49 < kanzure> .tw https://twitter.com/kanzure/status/917492046331555840 13:49 < yoleaux> @newscientist It's the other way around. Gene editing has excluded WADA from participating in sports & all too human competition. (@kanzure, in reply to tw:917382678294487040) 14:11 -!- c0rw1n_ [~c0rw1n@cpc109847-bagu17-2-0-cust223.1-3.cable.virginm.net] has joined ##hplusroadmap 14:18 < kanzure> 'enhancement by artificial mitochondria transfer' 14:34 -!- jaboja [~jaboja@jaboja.pl] has joined ##hplusroadmap 15:19 -!- augur [~augur@noisebridge130.static.monkeybrains.net] has joined ##hplusroadmap 15:55 -!- augur [~augur@noisebridge130.static.monkeybrains.net] has quit [Remote host closed the connection] 15:56 -!- augur [~augur@noisebridge130.static.monkeybrains.net] has joined ##hplusroadmap 16:01 < kanzure> .title https://news.ycombinator.com/item?id=15436858 16:01 < yoleaux> Report: TSMC's 3nm Fab Could Cost $20B | Hacker News 16:14 -!- aeiousomething [~aeiousome@gateway/vpn/privateinternetaccess/aeiousomething] has joined ##hplusroadmap 16:19 -!- Dumuzi- is now known as Dumuzi 16:22 < superkuh> I though the comments in the HN thread were significantly less informed than usual. Lots of falsehoods too. 16:34 -!- cluckj [~cluckj@static-173-59-27-112.phlapa.ftas.verizon.net] has joined ##hplusroadmap 17:11 -!- jaboja [~jaboja@jaboja.pl] has quit [Ping timeout: 248 seconds] 17:30 -!- rpifan [~rpifan@73.106.72.153] has joined ##hplusroadmap 17:49 -!- jaboja [~jaboja@jaboja.pl] has joined ##hplusroadmap 18:15 -!- augur [~augur@noisebridge130.static.monkeybrains.net] has quit [Remote host closed the connection] 18:20 -!- augur_ [~augur@noisebridge130.static.monkeybrains.net] has joined ##hplusroadmap 18:21 -!- augur_ [~augur@noisebridge130.static.monkeybrains.net] has quit [Remote host closed the connection] 18:22 -!- augur_ [~augur@noisebridge130.static.monkeybrains.net] has joined ##hplusroadmap 18:44 -!- mindsForge [~nak@75-172-169-191.phnx.qwest.net] has quit [Remote host closed the connection] 19:02 -!- augur_ [~augur@noisebridge130.static.monkeybrains.net] has quit [Remote host closed the connection] 19:03 -!- augur [~augur@noisebridge130.static.monkeybrains.net] has joined ##hplusroadmap 19:09 -!- jaboja [~jaboja@jaboja.pl] has quit [Ping timeout: 248 seconds] 19:23 < heath> superkuh: are you referring to the equivalences between manufacturers' stated sizes? 19:27 < superkuh> Yes. But it looks like voting has done it's job now. The mmanfrin thread used to be on top. 19:34 -!- superkuh [~superkuh@unaffiliated/superkuh] has left ##hplusroadmap ["the neuronal action potential is an electrical manipulation of reversible abrupt phase changes in the lipid bilayer"] 19:39 -!- augur [~augur@noisebridge130.static.monkeybrains.net] has quit [Remote host closed the connection] 19:40 -!- augur [~augur@192.195.83.130] has joined ##hplusroadmap 19:43 -!- yashgaroth [~yashgarot@2606:6000:cd4d:3300:f5e0:f867:a11d:8d52] has joined ##hplusroadmap 19:55 < nmz787> kanzure: what is WADA? 19:56 < kanzure> world anti-doping agency 19:56 < kanzure> they are the evil villains that test all the athletes 19:57 < kanzure> https://www.wada-ama.org/ 19:57 < nmz787> ah 19:58 < kanzure> WADA 2018 prohibited list https://www.wada-ama.org/sites/default/files/prohibited_list_2018_en.pdf 19:58 < nmz787> more reason to hate sports/sporting-industry 19:59 < kanzure> "The following, with the potential to enhance sport performance, are prohibited: 1. The use of polymers of nucleic acids or nucleic acid analogues. 2. The use of gene editing agents designed to alter genome sequences and/or the transcriptional or epigenetic regulation of gene expression. 3. The use of normal or genetically modified cells." 19:59 < nmz787> does it cover pre-natal mods? 19:59 < kanzure> bahahaha "the use of normal cells" is prohibited 19:59 < nmz787> i.e. ones parents induced/chose 20:00 < nmz787> I just saw some of the new blade runner online... and it was basically talking about killing GMO-humans 20:01 < kanzure> blade runner 2049 was a good movie, go see it 20:01 < nmz787> yeah I stopped watching and will go to theater 20:01 < kanzure> the original plot of blade runner was sort of bogus (and so is this one) but it's okay for what it is, got more right than ghost in shell at least 20:03 < kanzure> they struggle to resolve a philosophical problem ("non-real things are worthy of contempt") without really defining what real is (it should be: some sort of general abstraction over utility/ability and groundtruths; instead, it turns into something about how people feel or whatever? it's sort of dumb in this aspect.) 20:04 < kanzure> er i guess that's wrong; it's also proposed that "things that are self-reproducing are more real" except they neglect to mention that software can copy itself... 20:05 < nmz787> reprap went missing during the big eclipse/blackout I guess 20:19 -!- rpifan_ [~rpifan@73.106.75.5] has joined ##hplusroadmap 20:20 -!- rpifan [~rpifan@73.106.72.153] has quit [Ping timeout: 255 seconds] 20:21 -!- rpifan__ [~rpifan@73.106.74.231] has joined ##hplusroadmap 20:22 < kanzure> so... why isn't birth control prohibited by WADA? 20:22 < kanzure> estrogens don't do much i guess. hrm. 20:22 < kanzure> for athletic performance 20:23 < kanzure> so maybe doping is fine as long as it's designed to athletically inhibit you 20:24 -!- rpifan_ [~rpifan@73.106.75.5] has quit [Ping timeout: 248 seconds] 20:24 < nmz787> well, you could spoil someone's career if you got them pregnant, I guess 20:24 < nmz787> get morning sickness at the olypics 20:25 < kanzure> estrogen is negative for athletic performance. so again, it seems like the way around the rule is to claim that the dopant is inhibitory not supporting. 20:25 < nmz787> but then they would have to admit to being permiscuous 20:25 < nmz787> which could have negative reputation 20:28 -!- aeiousom1thing [~aeiousome@183.82.170.54] has joined ##hplusroadmap 20:28 -!- aeiousomething [~aeiousome@gateway/vpn/privateinternetaccess/aeiousomething] has quit [Ping timeout: 248 seconds] 20:29 -!- augur [~augur@192.195.83.130] has quit [Remote host closed the connection] 20:32 -!- augur [~augur@noisebridge130.static.monkeybrains.net] has joined ##hplusroadmap 20:34 -!- aeiousom1thing [~aeiousome@183.82.170.54] has quit [Ping timeout: 255 seconds] 20:36 -!- augur [~augur@noisebridge130.static.monkeybrains.net] has quit [Remote host closed the connection] 20:50 -!- Gurkenglas [~Gurkengla@dslb-094-223-135-191.094.223.pools.vodafone-ip.de] has quit [Ping timeout: 240 seconds] 20:54 -!- darsie [~username@84-114-73-160.cable.dynamic.surfer.at] has joined ##hplusroadmap 21:14 -!- archels [charl@unaffiliated/archels] has quit [Read error: Connection reset by peer] 21:14 -!- archels [charl@toad.stack.nl] has joined ##hplusroadmap 21:38 < heath> music: https://open.spotify.com/user/spotify/playlist/37i9dQZF1DWXLeA8Omikj7 21:47 -!- Malvolio [~Malvolio@unaffiliated/malvolio] has quit [Ping timeout: 258 seconds] 21:48 -!- augur [~augur@noisebridge130.static.monkeybrains.net] has joined ##hplusroadmap 21:49 -!- Malvolio [~Malvolio@unaffiliated/malvolio] has joined ##hplusroadmap 22:02 < nmz787> yashgaroth: is there a polymerase that slips and skips (produces deletions)? 22:02 < nmz787> kanzure: ^ 22:05 < jrayhawk> what? estrogen is anabolic 22:06 < jrayhawk> https://www.ncbi.nlm.nih.gov/pubmed/22278942 22:07 < jrayhawk> like, to the point where bodybuilders literally supplement with estrogen so that their crazy testosterone regimens actually work in the first place 22:08 -!- augur [~augur@noisebridge130.static.monkeybrains.net] has quit [Remote host closed the connection] 22:09 < jrayhawk> also i suspect the WADA is of fairly neutral value in a world where most experimentation on olympic-grade human conditioning is not published 22:09 < jrayhawk> if we could incentivize coaches and athletes to publish more often, getting rid of WADA might actually be meaningful 22:12 < yashgaroth> nmz787: not naturally occurring I don't think 22:13 -!- augur [~augur@noisebridge130.static.monkeybrains.net] has joined ##hplusroadmap 22:19 < jrayhawk> as it stands, we're just sparing athletes from the worst of abduction and experimentation within autocratic societies 22:27 -!- augur [~augur@noisebridge130.static.monkeybrains.net] has quit [Remote host closed the connection] 22:36 < jrayhawk> and endless series of pointlessly unpublished cronenberg monsters 22:38 -!- rpifan__ [~rpifan@73.106.74.231] has quit [Ping timeout: 248 seconds] 22:40 -!- yashgaroth [~yashgarot@2606:6000:cd4d:3300:f5e0:f867:a11d:8d52] has quit [Quit: Leaving] 22:41 -!- augur [~augur@noisebridge130.static.monkeybrains.net] has joined ##hplusroadmap 22:45 -!- augur [~augur@noisebridge130.static.monkeybrains.net] has quit [Remote host closed the connection] 23:08 < heath> Locked nucleic acid reagents for labelling nucleic acids https://www.google.com/patents/US8293684 23:12 -!- augur [~augur@noisebridge130.static.monkeybrains.net] has joined ##hplusroadmap 23:16 -!- augur [~augur@noisebridge130.static.monkeybrains.net] has quit [Remote host closed the connection] 23:20 < heath> Saliva-Based Biosensors: Noninvasive Monitoring Tool for Clinical Diagnostics http://dx.doi.org/10.1155/2014/962903 23:21 < heath> Nucleic acid approaches for detection and identification of biological warfare and infectious disease agents http://citeseerx.ist.psu.edu/viewdoc/download?doi=10.1.1.456.1561&rep=rep1&type=pdf 23:21 < heath> mentioned in http://dx.doi.org/10.1007/1-4020-3384-2 23:21 < heath> the patent: https://patentimages.storage.googleapis.com/f8/eb/9e/1933abc1dfc780/US20090242429A1.pdf 23:37 -!- augur [~augur@noisebridge130.static.monkeybrains.net] has joined ##hplusroadmap 23:46 -!- augur [~augur@noisebridge130.static.monkeybrains.net] has quit [Remote host closed the connection] 23:47 -!- augur [~augur@noisebridge130.static.monkeybrains.net] has joined ##hplusroadmap 23:50 -!- augur [~augur@noisebridge130.static.monkeybrains.net] has quit [Remote host closed the connection] 23:51 -!- augur [~augur@noisebridge130.static.monkeybrains.net] has joined ##hplusroadmap 23:57 -!- augur [~augur@noisebridge130.static.monkeybrains.net] has quit [Remote host closed the connection] --- Log closed Tue Oct 10 00:00:41 2017