--- Log opened Sun Dec 31 00:00:58 2017 00:17 -!- Malvolio [~Malvolio@unaffiliated/malvolio] has joined ##hplusroadmap 00:20 -!- darsie [~username@84-114-73-160.cable.dynamic.surfer.at] has quit [Ping timeout: 272 seconds] 01:07 < adlai> nmz787: the whole "m v f" thing is quite intriguing. your observation re, as kanzure summarized, "those made of meat", triggered my observation about how one particular media franchise is trying to steer things. 02:09 < maaku> can anyone recommend a way to lower pulse rate from dangerous levels after combining dexamfetamine with caffeine? 02:13 < adlai> maaku: from theoretical knowledge - safely administered, preferably prescribed, opiates. from practical experience - any relaxation technique which has worked for you personally in the past (eg, tea ritual + meditation) 02:15 < maaku> Thanks. But soemthing over the counter or herbal or home remedy would helpful 02:30 < adlai> poppy tea isn't herbal enough for you? iirc they are legal as a decorative plant in pretty much any jurisdiction that respects the right of an individual to tend a home garden 02:45 -!- poppingtonic [~brian@unaffiliated/poppingtonic] has joined ##hplusroadmap 05:29 -!- strages [uid11297@gateway/web/irccloud.com/x-mdqfonrczsxznfqo] has quit [Quit: Connection closed for inactivity] 06:06 -!- poppingtonic [~brian@unaffiliated/poppingtonic] has quit [Ping timeout: 240 seconds] 07:34 -!- hehelleshin [~talinck@174.97.113.184] has joined ##hplusroadmap 07:34 -!- ebowden_ [~ebowden@110.141.83.102] has joined ##hplusroadmap 07:35 -!- EnabrinTain [sid11525@gateway/web/irccloud.com/x-bjbvguaoidfvfmbs] has quit [Ping timeout: 248 seconds] 07:36 -!- ebowden [~ebowden@110.141.83.102] has quit [Ping timeout: 248 seconds] 07:37 -!- Guest14879 [~talinck@cpe-174-97-113-184.cinci.res.rr.com] has quit [Ping timeout: 248 seconds] 07:37 -!- juri_ [~juri@205.166.94.162] has quit [Ping timeout: 248 seconds] 07:37 -!- Burninate [~Burn@pool-96-241-130-178.washdc.fios.verizon.net] has quit [Ping timeout: 248 seconds] 07:38 -!- Burninate [~Burn@pool-96-241-130-178.washdc.fios.verizon.net] has joined ##hplusroadmap 07:38 -!- yoleaux [~yoleaux@xn--ht-1ia18f.nonceword.org] has quit [Ping timeout: 240 seconds] 07:39 -!- juri_ [~juri@205.166.94.162] has joined ##hplusroadmap 07:49 -!- EnabrinTain [sid11525@gateway/web/irccloud.com/x-llavjmgkirguxqnu] has joined ##hplusroadmap 08:24 -!- Gurkenglas [~Gurkengla@dslb-094-223-133-187.094.223.pools.vodafone-ip.de] has joined ##hplusroadmap 08:25 < kanzure> maaku: maaaaybe time to ween off the caffeine addiction 08:28 < kanzure> juul: do you know of any way i could source dNTPs at <$10/kg? 08:34 < kanzure> "Total biosynthesis of deoxynucleoside triphosphates using deoxynucleoside monophosphate kinases for PCR application" http://onlinelibrary.wiley.com/doi/10.1002/bit.21498/abstract 08:34 -!- drewbot [~cinch@54.162.220.167] has quit [Remote host closed the connection] 08:37 -!- ebowden_ [~ebowden@110.141.83.102] has quit [Ping timeout: 240 seconds] 08:39 -!- ebowden [~ebowden@110.141.83.102] has joined ##hplusroadmap 08:57 -!- mrdata_ [~mrdata@unaffiliated/mrdata] has joined ##hplusroadmap 09:00 -!- superkuh [~superkuh@unaffiliated/superkuh] has quit [Excess Flood] 09:00 -!- Douhet [~Douhet@unaffiliated/douhet] has quit [Ping timeout: 264 seconds] 09:00 -!- mrdata [~mrdata@unaffiliated/mrdata] has quit [Ping timeout: 264 seconds] 09:01 -!- Douhet [~Douhet@unaffiliated/douhet] has joined ##hplusroadmap 09:02 -!- superkuh [~superkuh@unaffiliated/superkuh] has joined ##hplusroadmap 09:10 -!- poppingtonic [~brian@unaffiliated/poppingtonic] has joined ##hplusroadmap 09:29 -!- poppingtonic [~brian@unaffiliated/poppingtonic] has quit [Ping timeout: 240 seconds] 09:38 -!- yoleaux [~yoleaux@xn--ht-1ia18f.nonceword.org] has joined ##hplusroadmap 09:46 -!- eb3c90 [~kvirc@host109-155-76-139.range109-155.btcentralplus.com] has joined ##hplusroadmap 09:48 -!- poppingtonic [~brian@unaffiliated/poppingtonic] has joined ##hplusroadmap 10:08 < kanzure> blort 10:36 -!- emeraldgreen [~user@188.227.115.178] has joined ##hplusroadmap 10:36 -!- emeraldgreen [~user@188.227.115.178] has quit [Client Quit] 10:41 -!- aeiousomething [~aeiousome@183.82.170.54] has joined ##hplusroadmap 10:53 -!- mrdata_ is now known as mrdata 10:54 < kanzure> .title http://www.introtcs.org/public/index.html 10:54 < yoleaux> Introduction to Theoretical Computer Science 11:01 -!- aeiousomething [~aeiousome@183.82.170.54] has quit [Ping timeout: 272 seconds] 11:54 -!- eb3c90 [~kvirc@host109-155-76-139.range109-155.btcentralplus.com] has quit [Ping timeout: 240 seconds] 12:49 -!- strages [uid11297@gateway/web/irccloud.com/x-pehrgssgpmvccelu] has joined ##hplusroadmap 12:53 -!- CheckDavid [uid14990@gateway/web/irccloud.com/x-sehvtazrbqiwbrdx] has joined ##hplusroadmap 13:03 -!- yashgaroth [~yashgarot@2606:6000:cd4d:3300:4114:351e:58e0:981] has joined ##hplusroadmap 13:24 -!- helleshin [~talinck@cpe-174-97-113-184.cinci.res.rr.com] has joined ##hplusroadmap 13:28 -!- hehelleshin [~talinck@174.97.113.184] has quit [Ping timeout: 256 seconds] 13:29 -!- darsie [~username@84-114-73-160.cable.dynamic.surfer.at] has joined ##hplusroadmap 14:03 < nmz787> adlai: can you define "m v f"? In the Star Wars movie, it seemed the only ugly ones were the puppet-looking aliens 14:04 < kanzure> no gender wars plz ugh 14:04 < kanzure> stop 14:05 < nmz787> ? 14:05 < nmz787> I don't have any idea how this relates to gender 14:06 < kanzure> huh? it's obvious-- both you and adlai have been talking about it; it's just off-topic and annoying. 14:07 < nmz787> I think my original comment was more like, if you have a really weird trait and are seen as an outsider to normal football-hooligan types, does that push the individual to be nerdier 14:07 < nmz787> not sure why gender was assumed to be important in this :/ 14:07 < kanzure> because you two keep talking about gender... look at the logs. 14:08 < nmz787> I don't know man 14:08 < nmz787> I just checked the logs all the way back to my original comment on the "gay" voice research that was recently posted 14:08 < nmz787> (I thought that was in here) 14:08 < nmz787> and again, that has nothing to do with gender 15:06 -!- drewbot [~cinch@54.234.24.92] has joined ##hplusroadmap 15:35 < kanzure> "Multiplexed imaging of high density libraries of RNAs with MERFISH and expansion microscopy" https://www.biorxiv.org/content/early/2017/12/22/238899 https://twitter.com/eboyden3/status/946480732750450688 16:22 -!- CheckDavid [uid14990@gateway/web/irccloud.com/x-sehvtazrbqiwbrdx] has quit [Quit: Connection closed for inactivity] 17:11 < kanzure> "Continuous directed evolution of aminoacyl-tRNA synthetases" https://www.nature.com/articles/nchembio.2474 17:15 < kanzure> 'conditional phage replication' https://pdfs.semanticscholar.org/0eb7/de41a936b7ad6b1d0bfef72a3e8ebf4c6920.pdfnnn 17:15 < kanzure> "Crystal structures reveal an elusive functional domain of pyrrolysyl-tRNA synthetase" https://www.nature.com/articles/nchembio.2497 17:27 < kanzure> "RNA polymerase tags to monitor multidimensional protein-protein interactions reveal pharmacological engagement of Bcl-2 proteins" http://pubs.acs.org/doi/abs/10.1021/jacs.7b06152 17:27 < kanzure> "We report the development of a new technology for monitoring multidimensional protein–protein interactions (PPIs) inside live mammalian cells using split RNA polymerase (RNAP) tags. In this new system, a protein-of-interest is tagged with an N-terminal split RNAP (RNAPN), and multiple potential binding partners are each fused to orthogonal C-terminal RNAPs (RNAPC). Assembly of RNAPN with ... 17:27 < kanzure> ...each RNAPC is highly dependent on interactions between the tagged proteins. Each PPI-mediated RNAPN-RNAPC assembly transcribes from a separate promoter on a supplied DNA substrate, thereby generating a unique RNA output signal for each PPI." 18:02 < kanzure> "Electron transfer between enzymes and electrodes" https://link.springer.com/chapter/10.1007/10_2017_42 18:20 < kanzure> "Fe-S clusters are ancient cofactors associated most commonly with enzymes that mediate electron transfer during energy conversion [18, 19]. Surprisingly, [Fe-S] clusters have also now been identified in nucleic acid processing enzymes such as glycosylases, helicases and polymerases, which have no obvious roles in electron transfer [12]. The [Fe-S] clusters appear to play a structural role in ... 18:21 < kanzure> ...these enzymes, though there is evidence that some of these enzymes also use the redox properties of the Fe-S cluster to execute DNA transactions, from binding and unwinding to the sensing of lesions [12]. The [4Fe-4S] cluster in the CTD of eukaryotic Pols δ and ζ helps to maintain the structural integrity of the second metal binding motif (CysB) for interactions with the regulatory ... 18:21 < kanzure> ...B-subunit (Pol31) [6, 7, 8]. In Pols α and ε, the situation is more complex, with evidence for the presence of Fe or Zn in the CysB of their CTDs [6, 8, 20]. We show here that Polε contains an Fe-S cluster directly within its active N-terminal exo-pol catalytic core. The Fe-S cluster is coordinated by cysteines on an “insertion” that is conserved in Pol2 orthologs, but is absent from ... 18:21 < kanzure> ...the catalytic subunits of Pols α, δ, and ζ. 18:21 < kanzure> "An iron-sulfur cluster in the polymerase domain of yeast DNA polymerase ε" https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4061903/ 18:21 -!- hehelleshin [~talinck@cpe-174-97-113-184.cinci.res.rr.com] has joined ##hplusroadmap 18:21 < kanzure> " 18:23 -!- superkuh [~superkuh@unaffiliated/superkuh] has quit [Excess Flood] 18:23 -!- helleshin [~talinck@cpe-174-97-113-184.cinci.res.rr.com] has quit [Ping timeout: 265 seconds] 18:24 -!- superkuh [~superkuh@unaffiliated/superkuh] has joined ##hplusroadmap 18:41 < kanzure> "Electrical and electrochemical monitoring of nucleic acid amplification" https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4350426/ 18:42 < kanzure> "Nucleic acid amplification by RCA on a silicon nanowire transistor was detected as a change in drain-source current in real time (Gao et al., 2013). Following hybridization of a target DNA with a probe DNA immobilized on the nanowire and a free RCA primer in a sandwich manner, RCA reaction takes place to synthesize a long ssDNA as a polyanion, leading to changes in the electronic state of ... 18:42 < kanzure> ...the silicon nanowire. The limit of detection was as low as 50 aM target DNA in an initial solution."nnnnn 18:42 < kanzure> "Liu and Yobas (2013) developed an electrical system for real-time PCR monitoring by ionic current rectification in a conical nanofluidic diode. Synthesized DNA amplicon electrostatically adsorbs on the nanopipette tips that modulates the ionic rectification. Since the adsorbed mass of DNA amplicons in the glass nanocapillaries is dependent on the number of cycles in real-time PCR, the system ... 18:42 < kanzure> ...can distinguish the mass concentration of the target DNA above 2.5 ng/μl." 18:50 -!- aeiousomething [~aeiousome@183.82.170.54] has joined ##hplusroadmap 18:51 < kanzure> .wik arylazopyrazole 18:51 < yoleaux> kanzure: Sorry, I couldn't find article. 18:55 -!- aeiousomething [~aeiousome@183.82.170.54] has quit [Ping timeout: 264 seconds] 19:02 < kanzure> "How to use photoswitchable cross-linker to reprogram proteins" http://diyhpl.us/~bryan/papers2/bio/How%20to%20use%20photoswitchable%20cross-linker%20to%20reprogram%20proteins%20-%202017.pdf 19:49 -!- Gurkenglas [~Gurkengla@dslb-094-223-133-187.094.223.pools.vodafone-ip.de] has quit [Read error: Connection reset by peer] 20:06 < kanzure> .title https://github.com/facebookresearch/wav2letter 20:06 < yoleaux> GitHub - facebookresearch/wav2letter: Facebook AI Research Automatic Speech Recognition Toolkit 21:35 < kanzure> https://www.aiche.org/sbe/conferences/metabolic-engineering-conference/2016/proceeding/paper/understanding-and-exploiting-enzyme-promiscuity-metabolic-engineering 22:31 -!- strages [uid11297@gateway/web/irccloud.com/x-pehrgssgpmvccelu] has quit [Quit: Connection closed for inactivity] 23:31 -!- yashgaroth [~yashgarot@2606:6000:cd4d:3300:4114:351e:58e0:981] has quit [Quit: Leaving] 23:46 < adlai> kanzure: i'm not at all genderwarring, i'm wondering about the post-singularity meatbag's attempts to tame evolution. if i should do this by mentioning phenotypes as such without referring to conventional genotype classifications, so be it; but both our NNNs know which qualia we mean. 23:46 < adlai> err, sorry - "meatbags'", plural. 23:46 < adlai> if theoretical discussion of evolution and its self-contortions is off-topic....... lol, i'll take my new-years hangover trolling elsewhere :) --- Log closed Mon Jan 01 00:00:59 2018