--- Log opened Fri Mar 23 00:00:26 2018 00:01 -!- ebowden [~ebowden@128.250.0.215] has quit [Read error: Connection reset by peer] 00:40 -!- ebowden [~ebowden@128.250.0.215] has joined ##hplusroadmap 00:52 -!- poppingtonic [~brian@unaffiliated/poppingtonic] has quit [Ping timeout: 256 seconds] 00:57 -!- Gurkenglas [~Gurkengla@dslb-178-005-162-043.178.005.pools.vodafone-ip.de] has joined ##hplusroadmap 00:58 -!- wrldpc1 [~ben@bai9536df7d.bai.ne.jp] has quit [Quit: wrldpc1] 01:06 -!- jtimon [~quassel@142.29.134.37.dynamic.jazztel.es] has quit [Ping timeout: 240 seconds] 01:54 -!- Gurkenglas [~Gurkengla@dslb-178-005-162-043.178.005.pools.vodafone-ip.de] has quit [Ping timeout: 256 seconds] 02:11 -!- aeiousomething [~aeiousome@27.59.226.166] has joined ##hplusroadmap 02:14 -!- red-001 [red@gateway/shell/elitebnc/x-hoepopqdsnrsiuxb] has quit [Ping timeout: 264 seconds] 02:21 -!- aeiousomething [~aeiousome@27.59.226.166] has quit [Read error: Connection reset by peer] 02:37 -!- red-001 [red@gateway/shell/elitebnc/x-lfpblxtcllrrjkin] has joined ##hplusroadmap 02:46 -!- aeiousomething [aeiousomet@gateway/vpn/privateinternetaccess/aeiousomething] has joined ##hplusroadmap 03:42 -!- jtimon [~quassel@142.29.134.37.dynamic.jazztel.es] has joined ##hplusroadmap 04:15 -!- slogger3141 [~slogger31@cpc136894-bsfd10-2-0-cust504.5-3.cable.virginm.net] has joined ##hplusroadmap 04:16 -!- slogger3141 [~slogger31@cpc136894-bsfd10-2-0-cust504.5-3.cable.virginm.net] has left ##hplusroadmap [] 04:44 < superkuh> https://zeptobars.com/en/read/HP-DeskJet-840c-thermal-inkjet-printing-head "weekend die-shot" 04:50 -!- CandleGlow [~CandleGlo@unaffiliated/candleglow] has quit [Quit: ...and all my responses they stay the same.] 05:00 < MrHindsight> those holes are enormous for ion beam drilling, laser can easily make those cleanly 05:02 < MrHindsight> plus that is 90's tech 05:07 -!- poppingtonic [~brian@unaffiliated/poppingtonic] has joined ##hplusroadmap 05:12 -!- poppingtonic [~brian@unaffiliated/poppingtonic] has quit [Ping timeout: 256 seconds] 05:32 -!- Gurkenglas [~Gurkengla@dslb-178-005-162-043.178.005.pools.vodafone-ip.de] has joined ##hplusroadmap 06:00 -!- darsie [~username@84-114-73-160.cable.dynamic.surfer.at] has joined ##hplusroadmap 06:06 -!- MrHindsight [~2020@unaffiliated/capthindsight] has quit [Ping timeout: 256 seconds] 06:13 < superkuh> Wow. https://onlinelibrary.wiley.com/ has blocked all 5 of my IPs I use for proxies. 06:14 < superkuh> The 'net is getting more and more segmented. 06:22 -!- MrHindsight [~2020@unaffiliated/capthindsight] has joined ##hplusroadmap 06:25 < kanzure> "Quantifying expansion microscopy with DNA origami expansion rulers" https://www.biorxiv.org/content/early/2018/02/14/265405 https://twitter.com/eboyden3/status/976886574771208200 06:25 < kanzure> "Multiplexed imaging of high-density libraries of RNAs with MERFISH and expansion microscopy" https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5859009/ https://twitter.com/eboyden3/status/976405932413538305 06:27 < kanzure> "Barcoded oligonucleotides ligated on RNA amplified for multiplex and parallel in-situ analyses" https://www.biorxiv.org/content/early/2018/03/20/281121 https://twitter.com/eboyden3/status/974045521928212481 06:29 < kanzure> "Expansion stimulated emission depletion microscopy (ExSTED)" https://www.biorxiv.org/content/early/2018/03/08/278937 https://twitter.com/eboyden3/status/973521208355577859 06:30 < kanzure> .tw https://twitter.com/eboyden3/status/973504244799197184 06:30 < yoleaux> Boston Museum of Science, Mar 28, 7PM: I will discuss "Cyborgs, Futurists & Transhumanism" with writer Mark O'Connell. Hosted by the Institute for Cross-Disciplinary Engagement at Dartmouth College, and its director, Marcelo Gleiser. https://www.mos.org/public-events/cyborgs-futurists-and-transhumanism?platform=hootsuite (@eboyden3) 06:34 < kanzure> "Temporally-precise single-cell resolution optogenetics" http://syntheticneurobiology.org/PDFs/17.11.shemesh.FULL.pdf 06:36 < kanzure> well that's fun. 06:37 < kanzure> "A hybridization-chain-reaction-based method for amplifying immunosignals" https://www.nature.com/articles/nmeth.4611 06:46 < kanzure> let's put 12 year olds in charge of the NIH budget. i am starting to really like the idea. 06:47 < kanzure> also the NASA budget 07:01 -!- MrHindsight is now known as CaptHindsight 07:06 -!- poppingtonic [~brian@unaffiliated/poppingtonic] has joined ##hplusroadmap 07:09 < kanzure> optical model of anti-aging: tissue culture microscopy and scoring of aging indicators, get tissue cultures that are naturally aged (i'm not convinced all the "aging-simulating" protocols are legitimately useful for aging studies), do optimization of virus payload for mutated proteins that seem to clear out optically-identifiable problems. do iteration loop things by mutating the virus ... 07:09 < kanzure> ...payloads. etc. you don't necessarily need to throw out a tissue culture once you run one batch of virus too. 07:10 < kanzure> orient yourself https://www.iers.org/IERS/EN/DataProducts/EarthOrientationData/eop.html 07:11 -!- poppingtonic [~brian@unaffiliated/poppingtonic] has quit [Ping timeout: 276 seconds] 07:19 < kanzure> "Functional germ cells from non-testicular adult stem cells: A dream or reality?" http://www.eurekaselect.com/156876/article 07:21 < CaptHindsight> life would be easier for nero 07:21 < CaptHindsight> oops 07:22 < CaptHindsight> life would be easier for neuroscientists if they could find some flat brains to study (2d) 07:28 < kanzure> agree 07:29 < kanzure> "Full-term development of mice from enucleated oocytes injected with cumulus cell nuclei" https://www.nature.com/articles/28615 07:30 < kanzure> "assisted oocyte activation" http://www.rbmojournal.com/article/S1472-6483(10)60313-6/abstract 07:30 -!- strages [uid11297@gateway/web/irccloud.com/x-pwfjvlzoljaaemug] has joined ##hplusroadmap 07:31 < kanzure> "Piezo-actuated mouse intracytoplasmic sperm injection" https://www.researchgate.net/profile/Tony_Perry2/publication/6414539_Piezo-actuated_mouse_intracytoplasmic_sperm_injection_ICSI/links/547d9d2b0cf2cfe203c21f24/Piezo-actuated-mouse-intracytoplasmic-sperm-injection-ICSI.pdf 07:32 < kanzure> hmm. 07:33 < kanzure> "Megabase-scale deletion using CRISPR/Cas9 to generate a fully haploid human cell line" https://genome.cshlp.org/content/24/12/2059.short 07:37 < CaptHindsight> http://www.machinedesign.com/motion-control/nano-and-micromotors-new-medical-delivery-systems 07:37 < CaptHindsight> has a bad link to the actual paper 07:38 < CaptHindsight> easy to make 07:41 < kanzure> why are these people doing it manually tho? wtf? 07:42 < CaptHindsight> http://mmbr.asm.org/content/80/1/161.full 07:42 < CaptHindsight> Biological Nanomotors with a Revolution, Linear, or Rotation Motion Mechanism 07:42 < kanzure> "Overexpression of STRA8, BOULE, and DAZL genes promotes goat bone marrow-derived mesenchymal stem cells in vitro transdifferentiation toward putative male germ cells" http://journals.sagepub.com/doi/abs/10.1177/1933719116654990 07:42 < kanzure> "Taken together, these results demonstrate that the overexpression of STRA8, BOULE, and DAZL was able to promote the transdifferentiation of gBMSCs to early goat germ cell–like cells in vitro, which probably enhanced maturation and progression through meiosis. This approach would be important to generating gametes for future basic science as well as for potential clinical applications." 07:43 -!- Malvolio [~Malvolio@unaffiliated/malvolio] has joined ##hplusroadmap 07:43 < kanzure> i'm a little confused about that "Functional germ cells" paper. it mentions that they aren't "fertile". but what the hell does that mean? did they even try intracytoplasmic injection, or were they just doing co-culturing fertilization attempts? 07:44 < CaptHindsight> https://pubs.acs.org/doi/ipdf/10.1021/nn507097k 07:44 < kanzure> why are they putting motors in a mouse stomach 07:45 < JayDugger> Jokes are too easy to make for that, must be a serious question. 07:46 < JayDugger> At a guess, easy animal model for tissue reactions? 07:47 < JayDugger> Yeah, in the abstract. Tests toxicity, with the additional effect that the motors get digested. 07:47 < kanzure> spermatid-like cells injected into oocytes seem to work, but spermatids aren't really that surprising, why not try something higher up the pipeline, http://www.cell.com/cell-stem-cell/abstract/S1934-5909(16)00018-7 07:50 -!- augur [~augur@2600:380:453b:7ad3:95:c1e0:f1a4:3118] has joined ##hplusroadmap 07:50 < CaptHindsight> edible motors, using the term loosely 07:51 < CaptHindsight> like a vinegar and baking soda "rocket" 08:05 -!- m4l3z [~m4l3z@2a01cb040226f200268eaf478c039961.ipv6.abo.wanadoo.fr] has joined ##hplusroadmap 08:11 < kanzure> "Fertilization of mouse oocytes using somatic cells as male germ cells" http://www.rbmojournal.com/article/S1472-6483(10)62037-8/fulltext 08:11 < kanzure> "Ooplasmic transfer" http://www.nejm.org/doi/pdf/10.1056/NEJM200203073461013 08:11 < kanzure> "To restore oocyte viability, ooplasm from a normal donor is removed with a micropipette and injected into an oocyte from the infertile woman. The resulting oocyte, which is fertilized by intracytoplasmic sperm injection, contains mitochondrial DNA from both women." 08:11 < kanzure> cool. 08:14 < kanzure> "Inability of mature oocytes to create functional haploid genomes from somatic cell nuclei" http://www.fertstert.org/article/S0015-0282(02)04537-5/fulltext 08:20 < kanzure> "Blastocysts derivation from somatic cell fusion with premature oocytes (prematuration somatic cell fusion)" https://onlinelibrary.wiley.com/doi/full/10.1111/dgd.12264 08:21 < kanzure> ^good paper. uses fluorescence to track the physical movements of genetic material after induced cell fusion. 08:26 < kanzure> they should have tried the overexpression of STRA8, BOULE, and DAZL in the somatic cells they were fusing to the oocytes. 08:44 < kanzure> reliability and efficiency of this stuff is way too low 08:46 -!- fred__tv [~fred__tv@host242-32-static.242-95-b.business.telecomitalia.it] has joined ##hplusroadmap 08:46 -!- fred__tv [~fred__tv@host242-32-static.242-95-b.business.telecomitalia.it] has left ##hplusroadmap ["Leaving"] 09:03 -!- ebowden [~ebowden@128.250.0.215] has quit [Ping timeout: 264 seconds] 09:10 -!- ebowden [~ebowden@128.250.0.194] has joined ##hplusroadmap 09:31 -!- solarnetone [~scott@rrcs-67-79-182-237.se.biz.rr.com] has quit [Ping timeout: 260 seconds] 09:48 -!- solarnetone [~scott@rrcs-67-79-182-237.se.biz.rr.com] has joined ##hplusroadmap 09:54 -!- solarnetone [~scott@rrcs-67-79-182-237.se.biz.rr.com] has quit [Ping timeout: 276 seconds] 09:56 -!- solarnetone [~scott@rrcs-67-79-182-237.se.biz.rr.com] has joined ##hplusroadmap 10:18 -!- m4l3z [~m4l3z@2a01cb040226f200268eaf478c039961.ipv6.abo.wanadoo.fr] has quit [Quit: Leaving] 11:10 -!- aeiousom1thing [aeiousomet@gateway/vpn/privateinternetaccess/aeiousomething] has joined ##hplusroadmap 11:11 -!- aeiousomething [aeiousomet@gateway/vpn/privateinternetaccess/aeiousomething] has quit [Ping timeout: 240 seconds] 11:22 -!- augur [~augur@2600:380:453b:7ad3:95:c1e0:f1a4:3118] has quit [Ping timeout: 276 seconds] 11:34 -!- CaptHindsight [~2020@unaffiliated/capthindsight] has quit [Remote host closed the connection] 11:36 -!- CaptHindsight [~2020@unaffiliated/capthindsight] has joined ##hplusroadmap 11:42 -!- poppingtonic [~brian@unaffiliated/poppingtonic] has joined ##hplusroadmap 11:46 -!- poppingtonic [~brian@unaffiliated/poppingtonic] has quit [Ping timeout: 240 seconds] 12:05 < CaptHindsight> https://onlinelibrary.wiley.com/doi/pdf/10.1002/anie.201309629?sharing_unavailable=unsupported_browser&sharing_referrer=http%3A%2F%2Fwww.iflscience.com%2Ftechnology%2Fnanomotors-steered-inside-living-human-cells-first-time%2F 12:06 < CaptHindsight> some yotubes of the above in action http://www.iflscience.com/technology/nanomotors-steered-inside-living-human-cells-first-time/ 12:30 -!- augur [~augur@2600:380:4520:3f16:28a0:78b2:8ffd:5a0b] has joined ##hplusroadmap 12:30 -!- bsm117532 [~mcelrath@173-9-124-61-NewEngland.hfc.comcastbusiness.net] has joined ##hplusroadmap 12:37 -!- bsm117532 [~mcelrath@173-9-124-61-NewEngland.hfc.comcastbusiness.net] has quit [Remote host closed the connection] 12:38 -!- bsm117532 [~mcelrath@173-9-124-61-NewEngland.hfc.comcastbusiness.net] has joined ##hplusroadmap 13:16 -!- augur [~augur@2600:380:4520:3f16:28a0:78b2:8ffd:5a0b] has quit [Remote host closed the connection] 14:03 -!- WeirdTolkienishF [~Weird@unaffiliated/weirdtolkienishf] has quit [Quit: Leaving] 14:04 -!- poppingtonic [~brian@unaffiliated/poppingtonic] has joined ##hplusroadmap 14:04 -!- poppingtonic [~brian@unaffiliated/poppingtonic] has quit [Client Quit] 14:05 -!- bsm117532 [~mcelrath@173-9-124-61-NewEngland.hfc.comcastbusiness.net] has quit [Ping timeout: 240 seconds] 14:26 -!- drewbot [~cinch@54.167.54.137] has joined ##hplusroadmap 14:47 -!- hehelleshin [~talinck@cpe-174-97-113-184.cinci.res.rr.com] has quit [Quit: Connection reset by bear] 14:49 -!- helleshin [~talinck@cpe-174-97-113-184.cinci.res.rr.com] has joined ##hplusroadmap 14:49 < CaptHindsight> want one https://www.youtube.com/watch?time_continue=16&v=QixTsqn7RBE 14:49 < CaptHindsight> New wearable brain scanner 14:56 < kanzure> .title 14:56 < yoleaux> New wearable brain scanner - YouTube 14:56 < kanzure> "an antibody specific for mouse germ cell nuclear antigen has recently been described [8]." 14:57 < kanzure> (mid 90s) 14:59 < kanzure> "Germ cell transplantation from large domestic animals into mouse testes" https://onlinelibrary.wiley.com/doi/full/10.1002/1098-2795%28200011%2957%3A3%3C270%3A%3AAID-MRD9%3E3.0.CO%3B2-Z 14:59 < kanzure> works but doesn't generate spermatozoa. hrm. 15:01 < kanzure> "Long-term survival of human spermatogonial stem cells in mouse testes" http://www.fertstert.org/article/S0015-0282(02)04345-5/abstract 15:10 < kanzure> testicular tissue xenotransplantation http://digital.csic.es/bitstream/10261/128746/6/RepFerDevelopment%2026(8)%20817-826%20(2014)%20POSTPRINT.pdf 15:13 < kanzure> human fetal testicular tissue xenotransplantation into mice http://www.jurology.com/article/S0022-5347(15)00209-8/abstract 15:14 < kanzure> .. and of course bioethicists are already worried about testicular xenotransplantation: https://link.springer.com/article/10.1007/s40592-015-0031-1 15:14 < kanzure> ("Ethical aspects of creating human–nonhuman chimeras capable of human gamete production and human pregnancy") 15:17 < kanzure> "Germ cell transplantation and neospermatogenesis" https://link.springer.com/chapter/10.1007/978-3-319-42396-8_20 15:18 < kanzure> "Testicular tissue xenografting [... is where] grafted testicular tissue grows and produces xenogenic sperm in a mouse host" 15:18 < kanzure> "Transplanted SSCs move towards the basal compartment of the seminiferous tubule to colonize, and subsequently undergo mitosis and meiosis to generate sperm" 15:45 < kanzure> huh, testicular tissue xenografting seems to work pretty well. 15:51 < kanzure> is there a way to do positive/negative selections on human cell cultures? other than cell sorting ideally. 15:56 -!- CandleGlow [~CandleGlo@unaffiliated/candleglow] has joined ##hplusroadmap 16:05 -!- hehelleshin [~talinck@cpe-174-97-113-184.cinci.res.rr.com] has joined ##hplusroadmap 16:07 -!- suhdood [~Thaelim@ool-4575d503.dyn.optonline.net] has joined ##hplusroadmap 16:08 -!- HEx1 [~HEx@hexwab.plus.com] has quit [Ping timeout: 246 seconds] 16:09 -!- helleshin [~talinck@cpe-174-97-113-184.cinci.res.rr.com] has quit [Ping timeout: 260 seconds] 16:17 -!- aeiousom1thing [aeiousomet@gateway/vpn/privateinternetaccess/aeiousomething] has quit [Ping timeout: 256 seconds] 16:20 -!- fluke`` [~user@193.165.236.4] has joined ##hplusroadmap 16:22 -!- fluke` [~user@193.165.236.4] has quit [Ping timeout: 260 seconds] 16:38 -!- HEx1 [~HEx@hexwab.plus.com] has joined ##hplusroadmap 17:12 -!- suhdood [~Thaelim@ool-4575d503.dyn.optonline.net] has quit [Quit: Leaving] 17:47 -!- CaptHindsight [~2020@unaffiliated/capthindsight] has quit [Quit: Leaving] 18:02 -!- CandleGlow [~CandleGlo@unaffiliated/candleglow] has quit [Read error: Connection reset by peer] 18:02 -!- CandleGlow [~CandleGlo@unaffiliated/candleglow] has joined ##hplusroadmap 18:05 -!- Draggy [~quassel@ip-2-205-48-228.web.vodafone.de] has joined ##hplusroadmap 18:05 -!- Draggy [~quassel@ip-2-205-48-228.web.vodafone.de] has left ##hplusroadmap [] 18:05 -!- Draggy [~quassel@ip-2-205-48-228.web.vodafone.de] has joined ##hplusroadmap 18:05 -!- Draggy [~quassel@ip-2-205-48-228.web.vodafone.de] has left ##hplusroadmap [] 18:07 -!- strages [uid11297@gateway/web/irccloud.com/x-pwfjvlzoljaaemug] has quit [Quit: Connection closed for inactivity] 18:43 -!- justan0theruser [~justanoth@unaffiliated/justanotheruser] has joined ##hplusroadmap 18:45 -!- justanotheruser [~justanoth@unaffiliated/justanotheruser] has quit [Ping timeout: 240 seconds] 19:24 -!- augur [~augur@192.195.83.130] has joined ##hplusroadmap 19:26 -!- augur [~augur@192.195.83.130] has quit [Remote host closed the connection] 19:32 -!- wrldpc1 [~ben@bai9536df7d.bai.ne.jp] has joined ##hplusroadmap 20:04 < kanzure> "Extremely scalable spiking neuronal network simulation code: From laptops to exascale computers" https://www.frontiersin.org/articles/10.3389/fninf.2018.00002/full 20:08 -!- Malvolio [~Malvolio@unaffiliated/malvolio] has quit [Quit: SCIENTISTS PREDICT ICE AGE] 20:11 < kanzure> "Each MPI process receives the global ids (GIDs) of all neurons that emitted a spike since the last MPI communication took place, and each thread needs to determine the thread-local neurons to which it needs to deliver the events." 20:11 < kanzure> blargh what 20:16 -!- strages [uid11297@gateway/web/irccloud.com/x-nblsznlqkatnmlcf] has joined ##hplusroadmap 20:24 -!- wrldpc1 [~ben@bai9536df7d.bai.ne.jp] has quit [Ping timeout: 240 seconds] 20:26 -!- wrldpc1 [~ben@sp49-98-147-204.msd.spmode.ne.jp] has joined ##hplusroadmap 20:29 -!- wrldpc1_ [~ben@sp49-98-147-204.msd.spmode.ne.jp] has joined ##hplusroadmap 20:30 -!- wrldpc1 [~ben@sp49-98-147-204.msd.spmode.ne.jp] has quit [Ping timeout: 264 seconds] 20:30 -!- wrldpc1_ is now known as wrldpc1 20:31 -!- suhdood_ [~Thaelim@ool-4575d503.dyn.optonline.net] has joined ##hplusroadmap 20:36 -!- wrldpc1_ [~ben@sp49-98-147-204.msd.spmode.ne.jp] has joined ##hplusroadmap 20:36 < kanzure> hmm 20:37 < nmz787> so how bout that nucleus direct-injection? 20:37 < nmz787> ever search that? 20:37 -!- wrldpc1 [~ben@sp49-98-147-204.msd.spmode.ne.jp] has quit [Ping timeout: 260 seconds] 20:37 -!- wrldpc1_ is now known as wrldpc1 20:37 < kanzure> hm? 20:38 < kanzure> intracytoplasmonic injection? 20:38 < nmz787> ha, sci-bay.org is already down 20:38 < nmz787> :( 20:38 < nmz787> no, injecting DNA solution into the nucleus 20:38 < kanzure> what dna? 20:39 < nmz787> .title https://www.ncbi.nlm.nih.gov/pubmed/18243328 20:39 < yoleaux> An automated system for intracellular and intranuclear injection. - PubMed - NCBI 20:39 < nmz787> kanzure: that synDNA yo 20:41 < nmz787> "automated system capable of injecting up to 600 oocytes per hour" "intranuclear injection of cDNA gave a rate of expression >50%" 20:41 < kanzure> how does this work, again? you flood the cell with dna or just a single molecule of dna..? 20:43 < nmz787> "Oocytes were injected 2 ng of cDNA per oocyte in 10 nl of water" 20:46 < nmz787> they used a fine syringe pump with a glass syringe having a stainless steel piston, backfilled with mineral oil, which was connected to a pipette tip they pulled from glass capillary 20:46 < nmz787> the pipette tips were apparently only 40 microns OD 20:46 < nmz787> which seems way too big for nucleus 20:46 < nmz787> but maybe xenopus nuclei are huge? 20:47 -!- wrldpc1_ [~ben@sp49-98-147-204.msd.spmode.ne.jp] has joined ##hplusroadmap 20:47 -!- suhdood_ [~Thaelim@ool-4575d503.dyn.optonline.net] has quit [Remote host closed the connection] 20:47 < nmz787> I'd be uploaded to a squid 20:47 < nmz787> that seems legit 20:49 -!- wrldpc1 [~ben@sp49-98-147-204.msd.spmode.ne.jp] has quit [Ping timeout: 260 seconds] 20:49 -!- wrldpc1_ is now known as wrldpc1 20:50 < nmz787> .title https://pubs.acs.org/doi/abs/10.1021/nl901384x 20:50 < yoleaux> nmz787: Sorry, that doesn't appear to be an HTML page. 20:50 < nmz787> FluidFM: Combining Atomic Force Microscopy and Nanofluidics in a Universal Liquid Delivery System for Single Cell Applications and Beyond 20:50 < nmz787> hmm 20:50 < nmz787> fenn ^ 20:50 < nmz787> that's dip-pen litho + whack-a-mole 20:51 < nmz787> "a game for the whole family" 20:51 < nmz787> "something the littlest to the oldest will enjoy playing" 20:54 -!- wrldpc1_ [~ben@sp49-98-147-204.msd.spmode.ne.jp] has joined ##hplusroadmap 20:56 -!- wrldpc1 [~ben@sp49-98-147-204.msd.spmode.ne.jp] has quit [Ping timeout: 264 seconds] 20:56 -!- wrldpc1_ is now known as wrldpc1 20:59 -!- wrldpc1_ [~ben@sp49-98-147-204.msd.spmode.ne.jp] has joined ##hplusroadmap 21:00 -!- wrldpc1 [~ben@sp49-98-147-204.msd.spmode.ne.jp] has quit [Ping timeout: 240 seconds] 21:00 -!- wrldpc1_ is now known as wrldpc1 21:02 < kanzure> someone tried dna + sperm injected into an oocyte and that seemed to sorta work http://science.sciencemag.org/content/284/5417/1180 21:02 < kanzure> not sure why they didn't try that during blastomere stage. maybe it's too difficult to catch. 21:03 -!- wrldpc1_ [~ben@sp49-98-147-204.msd.spmode.ne.jp] has joined ##hplusroadmap 21:05 -!- wrldpc1 [~ben@sp49-98-147-204.msd.spmode.ne.jp] has quit [Ping timeout: 276 seconds] 21:07 -!- wrldpc1 [~ben@sp49-98-147-204.msd.spmode.ne.jp] has joined ##hplusroadmap 21:07 -!- wrldpc1_ [~ben@sp49-98-147-204.msd.spmode.ne.jp] has quit [Ping timeout: 240 seconds] 21:12 -!- wrldpc1 [~ben@sp49-98-147-204.msd.spmode.ne.jp] has quit [Ping timeout: 256 seconds] 21:16 < kanzure> good review of testis-mediated gene transfer stuff https://onlinelibrary.wiley.com/doi/full/10.1111/j.1447-0578.2006.00117.x 21:20 -!- drewbot [~cinch@54.167.54.137] has quit [Remote host closed the connection] 21:21 < kanzure> "... our preliminary trial of repeated injections (up to six injections) of plasmid/liposome (FuGENE6) complex failed to improve the TMGT system.23 The choice of reagents used for gene transfer may be important in improving TMGT: in particular, the use of DMRIE‐C and TransFect appears promising.20" 21:22 -!- ebowden [~ebowden@128.250.0.194] has quit [Read error: Connection reset by peer] 21:22 -!- ebowden [~ebowden@128.250.0.194] has joined ##hplusroadmap 21:25 < kanzure> .wik pronuclear injection 21:25 < yoleaux> "Microinjection is the use of a glass micropipette to inject a liquid substance at a microscopic or borderline macroscopic level." — https://en.wikipedia.org/wiki/Pronuclear_injection 21:25 < kanzure> erm.. 21:29 < kanzure> direct delivery of mammalian artificial chromosomes into mouse oocyte ... 21:29 < kanzure> ...https://www.researchgate.net/profile/Carl_Perez3/publication/5819227_Pronuclear_microinjection_of_purified_artificial_chromosomes_for_generation_of_transgenic_mice_pick-and-inject_technique/links/591a1cdba6fdccb149f3821f/Pronuclear-microinjection-of-purified-artificial-chromosomes-for-generation-of-transgenic-mice-pick-and-inject-technique.pdf 21:30 < kanzure> oh, it's their protocol. that's nice of them to write that down. 21:32 < kanzure> yeah efficiency of all this stuff is too low to be useful 21:35 < kanzure> it's not clear to me how much engineering has really gone into intracytoplasmic sperm injection.. for example, there's many, many stains available. are they using all those stains for visualizing current state of oocyte? spindle formation? meiosis lifecycle status? chromosome labeling and tracking? oocyte maturity measurement? 21:36 < kanzure> and are they delivering any co-factors (such as proteins or mRNA or whatever) to improve outcomes.. 21:37 < kanzure> you should also be able to do the same with sperm of course.. or even spermatids.. e.g. why don't i see lots of fluorescent sperm or fluorescently-labeled chromosomes in sperms in any of the diagrams from these papers? how does the operator know that the sperm cell they picked is a good choice...? 21:39 < kanzure> also it sounds like these people are manully doing intracytoplasmic injection.. so that means they probably only do it on one or two cells total because people get tired, right? but really if it has low efficiency then you want to be doing this hundreds of times on all the available cells. 21:40 < kanzure> robotic intracytoplasmic injection system thingy https://www.ncbi.nlm.nih.gov/pubmed/21521663 21:42 < kanzure> and here's a patent from "the inventor of intracytoplasmic injection" for microfluidic automation of the procedure? not sure why this isn't picked up by a company yet: https://flintbox.com/public/project/24702/ 21:42 < kanzure> .title https://patents.google.com/patent/WO2013158658A1 21:42 < yoleaux> WO2013158658A1 - Automated intracytoplasmic sperm injection assisted fertilization system - Google Patents 22:12 -!- darsie [~username@84-114-73-160.cable.dynamic.surfer.at] has quit [Ping timeout: 256 seconds] 22:52 -!- ebowden [~ebowden@128.250.0.194] has quit [Read error: Connection reset by peer] 22:52 -!- ebowden [~ebowden@128.250.0.194] has joined ##hplusroadmap 23:02 -!- linux_ [~linux@114.4.79.10] has joined ##hplusroadmap --- Log closed Sat Mar 24 00:00:27 2018