--- Log opened Sat Apr 14 00:00:48 2018 00:06 -!- augur [~augur@2600:380:853b:6ea1:e568:fe8c:46ca:5b86] has joined ##hplusroadmap 00:07 -!- augur_ [~augur@2600:380:853b:6ea1:5599:6a97:d631:e87e] has joined ##hplusroadmap 00:11 -!- augur [~augur@2600:380:853b:6ea1:e568:fe8c:46ca:5b86] has quit [Ping timeout: 276 seconds] 00:16 -!- augur_ [~augur@2600:380:853b:6ea1:5599:6a97:d631:e87e] has quit [Remote host closed the connection] 00:17 -!- augur [~augur@2600:380:853b:6ea1:5599:6a97:d631:e87e] has joined ##hplusroadmap 00:18 -!- l_wl [~l_wl@pool-173-66-205-23.washdc.fios.verizon.net] has quit [Quit: gone for now but not for good] 00:19 -!- l_wl [~l_wl@pool-173-66-205-23.washdc.fios.verizon.net] has joined ##hplusroadmap 00:23 -!- augur [~augur@2600:380:853b:6ea1:5599:6a97:d631:e87e] has quit [Ping timeout: 245 seconds] 00:55 -!- Gurkenglas [~Gurkengla@dslb-188-102-077-078.188.102.pools.vodafone-ip.de] has joined ##hplusroadmap 00:59 -!- ExeciN [ExeciN@bnc.stormbit.net] has joined ##hplusroadmap 01:08 -!- strages [uid11297@gateway/web/irccloud.com/x-lklioekknoipwyjp] has quit [Quit: Connection closed for inactivity] 01:13 -!- darsie [~username@84-114-73-160.cable.dynamic.surfer.at] has joined ##hplusroadmap 01:14 -!- aeiousomething [~aeiousome@124.123.14.99] has joined ##hplusroadmap 01:14 -!- NikopolSohru [~NSohru@185.212.171.198] has joined ##hplusroadmap 01:26 < ebowden> MrHindsight, what are these? 01:43 -!- aeiousomething [~aeiousome@124.123.14.99] has quit [Ping timeout: 256 seconds] 02:26 -!- gabbar1947 [uid205515@gateway/web/irccloud.com/x-gqpcqlrojfgirgbd] has joined ##hplusroadmap 02:59 -!- l_wl [~l_wl@pool-173-66-205-23.washdc.fios.verizon.net] has quit [Quit: gone for now but not for good] 03:00 -!- l_wl [~l_wl@pool-173-66-205-23.washdc.fios.verizon.net] has joined ##hplusroadmap 03:48 -!- jtimon [~quassel@142.29.134.37.dynamic.jazztel.es] has joined ##hplusroadmap 04:25 -!- ebowden [~ebowden@128.250.0.209] has quit [Remote host closed the connection] 04:25 -!- ebowden [~ebowden@128.250.0.209] has joined ##hplusroadmap 04:49 -!- sachy [~sachy@78.108.102.220] has joined ##hplusroadmap 05:01 -!- darsie [~username@84-114-73-160.cable.dynamic.surfer.at] has quit [Quit: KVIrc 4.2.0 Equilibrium http://www.kvirc.net/] 05:47 -!- gabbar1947 [uid205515@gateway/web/irccloud.com/x-gqpcqlrojfgirgbd] has quit [Quit: Connection closed for inactivity] 06:17 -!- aeiousomething [~aeiousome@124.123.14.99] has joined ##hplusroadmap 07:09 < kanzure> fenn: well in absence of ratcheting it may be possible to find a polymerase that is highly reliably-slow, and then monitor it with sequencing-by-synthesis fluorescence events 07:12 < kanzure> MrHindsight: dna data storage might be a fad but it's where you'll get the investment dollars to make reliable cheap biologically-relevant dna synthesis 07:23 < kanzure> uh.. should it be possible to ligate a polymerase's nascent molecule to the end of the template molecule? 07:24 < kanzure> during synthesis 07:26 < kanzure> combined with sequencing-by-synthesis fluorescence events, you could keep copying the same sequence indefinitely while also modifying it with interference, and also you can save the existing changes each "pass" over the complete sequence. 07:39 < kanzure> "Nonenzymatic ligation of DNA with a reversible step and a final linkage that can be used in PCR" https://onlinelibrary.wiley.com/doi/full/10.1002/cbic.201200167 07:41 < kanzure> this is superior to my method from yesterday with rolling circle amplification because in those reactions you have to get the modifications right every single time you go around the loop, which is probabilistically unlikely with increasing number of changes. 07:41 < kanzure> however, if you can preserve the modifications each round (by ligating template and nascent molecule) then you are only constrained by the data repeat length interval really 07:51 -!- hazirafel [~hazirafel@bzq-109-66-0-197.red.bezeqint.net] has joined ##hplusroadmap 08:12 < kanzure> er.. i might have misunderstood something about PCR actually. 08:28 -!- darsie [~username@84-114-73-160.cable.dynamic.surfer.at] has joined ##hplusroadmap 08:31 -!- CheckDavid [uid14990@gateway/web/irccloud.com/x-nudqzwnumvoamdwr] has joined ##hplusroadmap 08:38 -!- strages [uid11297@gateway/web/irccloud.com/x-femoaglsoflporet] has joined ##hplusroadmap 08:41 -!- darsie [~username@84-114-73-160.cable.dynamic.surfer.at] has quit [Quit: KVIrc 4.2.0 Equilibrium http://www.kvirc.net/] 09:00 -!- darsie [~username@84-114-73-160.cable.dynamic.surfer.at] has joined ##hplusroadmap 09:00 -!- darsie is now known as Guest35968 09:06 -!- Guest35968 [~username@84-114-73-160.cable.dynamic.surfer.at] has quit [Quit: KVIrc 4.2.0 Equilibrium http://www.kvirc.net/] 09:06 -!- darsie42 [~username@84-114-73-160.cable.dynamic.surfer.at] has joined ##hplusroadmap 09:11 -!- darsie42 [~username@84-114-73-160.cable.dynamic.surfer.at] has quit [Client Quit] 09:11 -!- darsie42 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quit [Read error: Connection reset by peer] 12:33 < nmz787> fenn: I was thinking that if you zapped voltage fast enough, faster than incorporation time, you might be guaranteed to induce perturbation 12:35 < nmz787> fenn: also I was thinking symmetric pulse-trains probably is too simplistic... my guess is you need some specific pulse-train of varying duty-cycle, and possible modulated frequency as well (i.e. a 10 ns pulse in + voltage... 5 ns pulse in - direction, followed by 3 ns pulse in + direction... so on and so forth) 12:36 < kanzure> i think we should send 'em another reply 13:03 -!- aeiousomething [~aeiousome@124.123.14.99] has quit [Quit: leaving] 13:10 < kanzure> "In an X Files season finale, aliens weaponize CRISPR to attack the human immune system" oh okay 13:12 < nmz787> bidding currently at $305 for this ~50 micron resolution 3D printer which has pretty good reviews... people report ~$400... buy-it-now for $530 https://www.ebay.com/itm/Anycubic-Photon-3D-Printer-405nm-UV-Resin-SLA-DLP-Light-Cure-2-8-LCD-FEP-US/273149463714 13:12 < nmz787> .title 13:12 < yoleaux> Anycubic Photon 3D Printer 405nm UV Resin SLA/DLP Light-Cure 2.8" LCD FEP US | eBay 13:13 < kanzure> ther's a lot of 3d printers that do dlp these days. have you checked their prices? 13:20 < nmz787> nah, just heard about this one from sebastian cocioba's facebook posts on it recently 13:20 < ebowden> https://www.youtube.com/watch?v=-qRp4wERAoY 13:21 < ebowden> My favourite point has to be "loyal government". 13:23 < nmz787> until the cement develops a hairline crack and water starts dripping? 13:24 < ebowden> This is an ad I was served for god knows what reason. 13:24 < ebowden> Not sure why, but it just SCREAMED dodgy to me. 13:24 < kanzure> why are you watching ads 13:28 < ebowden> I normally don't/ 13:28 < ebowden> I normally don't. 13:28 < ebowden> But in this case, I just couldn't look away. 13:29 -!- hazirafel 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[~l_wl@pool-173-66-205-23.washdc.fios.verizon.net] has joined ##hplusroadmap 19:24 -!- l_wl_ [~l_wl@pool-173-66-205-23.washdc.fios.verizon.net] has quit [Ping timeout: 264 seconds] 19:29 < kanzure> bloort 19:38 -!- ebowden [~ebowden@128.250.0.209] has quit [Ping timeout: 260 seconds] 20:27 -!- jtimon [~quassel@142.29.134.37.dynamic.jazztel.es] has quit [Ping timeout: 256 seconds] 20:36 < kanzure> don't be boring 20:37 < kanzure> .wik mutant mastermind 20:37 < yoleaux> kanzure: Sorry, that command (.wik) crashed. 20:37 < kanzure> .wik mutants & masterminds 20:37 < yoleaux> "In biology and especially genetics, a mutant is an organism or a new genetic character arising or resulting from an instance of mutation, which is an alteration of the DNA sequence of a gene or chromosome of an organism. The natural occurrence of genetic mutations is integral to the process of evolution." — https://en.wikipedia.org/wiki/Mutants 20:37 -!- augur [~augur@noisebridge130.static.monkeybrains.net] has quit [Remote host closed the connection] 20:38 -!- augur [~augur@noisebridge130.static.monkeybrains.net] has joined ##hplusroadmap 20:43 -!- augur [~augur@noisebridge130.static.monkeybrains.net] has quit [Ping timeout: 260 seconds] 21:04 -!- augur [~augur@noisebridge130.static.monkeybrains.net] has joined ##hplusroadmap 21:07 -!- augur [~augur@noisebridge130.static.monkeybrains.net] has quit [Remote host closed the connection] 21:08 -!- augur [~augur@noisebridge130.static.monkeybrains.net] has joined ##hplusroadmap 21:13 -!- augur [~augur@noisebridge130.static.monkeybrains.net] has quit [Ping timeout: 265 seconds] 21:24 -!- Guest12618 [~augur@noisebridge130.static.monkeybrains.net] has joined ##hplusroadmap 21:31 -!- l_wl [~l_wl@pool-173-66-205-23.washdc.fios.verizon.net] has quit [Ping timeout: 240 seconds] 21:40 -!- aeiousomething [~aeiousome@124.123.14.99] has quit [Ping timeout: 260 seconds] 22:03 -!- aeiousomething [~aeiousome@124.123.14.99] has joined ##hplusroadmap 22:04 -!- aeiousomething [~aeiousome@124.123.14.99] has quit [Client Quit] 22:05 -!- aeiousomething [~aeiousome@124.123.14.99] has joined ##hplusroadmap 22:14 -!- aeiousomething [~aeiousome@124.123.14.99] has quit [Ping timeout: 264 seconds] 22:15 -!- CandleGlow [~CandleGlo@unaffiliated/candleglow] has quit [Quit: ...and all my responses they stay the same.] 22:49 -!- Guest12618 [~augur@noisebridge130.static.monkeybrains.net] has quit [Remote host closed the connection] 23:15 -!- aeiousomething [~aeiousome@124.123.14.99] has joined ##hplusroadmap 23:27 -!- strages [uid11297@gateway/web/irccloud.com/x-femoaglsoflporet] has quit [Quit: Connection closed for inactivity] 23:30 -!- Malvolio [~Malvolio@unaffiliated/malvolio] has quit [Quit: Malvolio] 23:42 -!- aeiousomething [~aeiousome@124.123.14.99] has quit [Ping timeout: 260 seconds] --- Log closed Sun Apr 15 00:00:48 2018