--- Log opened Tue May 29 00:00:30 2018
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07:03 < kanzure> https://globenewswire.com/news-release/2018/05/28/1512796/0/en/Myriad-Genetics-Signs-Definitive-Agreement-to-Acquire-Counsyl-Inc.html
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07:16 < kanzure> https://twitter.com/ElonBob
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13:14 < kanzure> hmm.
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13:26 < kanzure> .wik anencephalic clones
13:26 < yoleaux> "NetHack is a single-player roguelike video game originally released in 1987 with ASCII graphics. It is a descendant of an earlier game called Hack (1982), which is a clone of Rogue (1980)." — https://en.wikipedia.org/wiki/NetHack
13:26 < kanzure> uhh.
13:28 < JayDugger> Ha, ha, ha.
13:28 < JayDugger> Sounds correct.
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14:06 < fenn> runs on headless
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15:33 < kanzure> .title http://2018.oshwa.org/speakers/
15:33 < yoleaux> Speakers – 2018 Open Source Hardware Summit
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18:04 < kanzure> .tw https://twitter.com/AkosNyerges/status/1000730939339755520
18:04 < yoleaux> Establishing a cell-free Vibrio natriegens expression system & simplified crude extract preparation protocol that achieved >260μg/mL of super-folder GFP in a small-scale batch reaction after 3 h https://www.biorxiv.org/content/early/2018/05/26/331645 @biorxivpreprint #synbio #cellfree #Vibrio #natriegens (@AkosNyerges)
18:05 < kanzure> https://hackaday.com/2018/05/29/tiny-25-spectrometer-aims-to-identify-materials-with-ease/
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18:37 < nmz787> sup
18:45 < kanzure> everything on fire, all good
18:45 < kanzure> fib status?
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19:44 < nmz787> kanzure: I asked Adam McCombs what his hourly/daily rate is to get my SEM and FIB up and running
19:44 < nmz787> (earlier this morning)
19:44 < nmz787> FIB is in the garage.. I need to go empty the water from the dehumidifer in there.
19:45 < nmz787> (FIB is in it's crate still)
19:51 < nmz787> yashgaroth: have you heard of this stuff, supposed to be better than lipofectamine and fugene https://www.polyplus-transfection.com/products/peipro-hq/
19:53 < yashgaroth> is that according to someone other than the manufacturer
19:54 < nmz787> if a salesguy counts, he's a family friend though...
19:55 < yashgaroth> probably works fine for in vitro stuff
19:55 < nmz787> apparently 30-50 L at a time in pharma batches
19:55 < nmz787> of cell transfection
19:55 < nmz787> at a time
19:55 < nmz787> was asking him about sales re electroporator
19:56 < yashgaroth> yeah that quickly becomes cost-prohibitive with lipofection
19:56 < nmz787> and they have GMP stuff
19:56 < nmz787> so the pharma companies can use it for packaged treatments
19:56 < nmz787> so I guess they can't filter/extract it easily enough from the final products?
19:56 < nmz787> or it's cheaper than filtering, to buy GMP
19:57 < nmz787> seemed like he said it required at least 10 mins 'complexing' time
19:57 < yashgaroth> GMP doesn't mean it's safe for injection, just that people looked at it and signed 400 documents
19:57 < nmz787> not sure if that means an electroporator would have to also do 30-50 L in 10 mins
19:57 < nmz787> or if there's some other time that he wasn't aware of
19:58 < yashgaroth> chemical transfection is gonna be way better for batches that size
19:58 < nmz787> also this is pretty cool, I think it's a fluidized bed setup for cell culture on micro/mini spheres
19:58 < nmz787> https://shop.pall.com/us/en/biotech/cell-culture/bioreactors/icellis-bioreactor-zidhw7uq21l
19:58 < nmz787> (adherent cells)
19:59 < nmz787> yashgaroth: well for an electroporator to do it, I think it'd have to be in-line, i.e. two pipes (one cells, one DNA) meet and then after the Y junction there'd be some electrodes to pulsing continuously, maybe you'd pulse the fluid flow in unison
20:00 < nmz787> so it'd just be a matter of calculating if that'd be possible to do in 10 mins
20:00 < nmz787> (while beating the transformation efficiency, or price)
20:02 < yashgaroth> that sounds like a pain in the ass when a tech can just dump a bunch of PEI in, but for cell therapy stuff maybe since the cells are going right back into the body
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20:18 < nmz787> 35 PCR cycles in 25 minutes with this magnetic-induction heated and forced-air cooled "most portable" qPCR machine: http://www.quantabio.com/products/pcr/instrumentation/products-pcr-instrumentation-q
20:37 < kanzure> is there still not an actual plan for fixing academic publishing?
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20:41 < kanzure> root of the problem seems to be journal-brands that are used for career promotion
20:42 < kanzure> could do prediction market + airdrop of tokens to existing researchers/authors + monetized by grants from different research funding agencies.. accurate reviewers would over time accrue mo-- .. eh whatever. i don't like prediction markets. and i'm not paul sztorc.
20:43 < kanzure> you could do: papers get endorsed by journal editors (or at least, they are currently journal editors, but might be called something else in the future) using public-key cryptography magic.   this data is attached to the paper itself. then you could crawl the web of science and accumulate total scores in a web-of-trust sort of way.
20:44 < kanzure> unfortunately journal brands are not currently synonymous with the set of editors running the journal itself, so i don't think you could convince people to do it that way
20:47 < kanzure> researchgate and academia.edu have messaging systems for tokendrop (or use email)
20:50 < nmz787> wait for sci-hub to disrupt it all to shit?
20:50 < nmz787> or failing shit
20:50 < nmz787> compost
20:51 < kanzure> scihub is happy where it's at right now, she isn't going to try more things
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21:05 < kanzure> maybe just convince everyone that closed-access publication is literally academic fraud
21:09 < kanzure> another interesting way to do this would be to publicize an 'academic transition event' or 'migration date' and incentivize the wholesale rejection of traditional journals by some currency system.. the deal would be that early adopters (scholars) would be given some tokens (and proportionally less if they are late adopters) to cover for the costs of their career risk or opportunity cost; and ...
21:09 < kanzure> ...their tokens get revoked if they continue to publish in closed-access journals. furthermore, their tokens would be monetized by various science agencies that wish to accelerate the transition to an open-access society.
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21:14 < kanzure> also these tokens would have to be locked for some n-year period before anyone could sell from the monetization pool (monetization pool funded by grant agencies etc)
21:15 < kanzure> .. which might complicate the whole thing, since the cost of foregoing career progression can be quite large for a lot of people.
21:15 < kanzure> well, i mean, nature-boosted career progression
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--- Log closed Wed May 30 00:00:31 2018