--- Log opened Sat Feb 23 00:00:57 2019
00:32 -!- decal [decal@ma.sdf.org] has joined ##hplusroadmap
00:36 -!- kuldeep [~kuldeep@unaffiliated/kuldeepdhaka] has quit [Read error: Connection reset by peer]
00:37 -!- kuldeep [~kuldeep@unaffiliated/kuldeepdhaka] has joined ##hplusroadmap
01:09 < superkuh> It's from a crackpot.
01:09 < superkuh> Guy also designed a 'gravity wave' drive.
01:52 -!- Gurkenglas [~Gurkengla@unaffiliated/gurkenglas] has joined ##hplusroadmap
03:44 -!- Gurkenglas [~Gurkengla@unaffiliated/gurkenglas] has quit [Ping timeout: 255 seconds]
03:51 -!- Urchin [~urchin@unaffiliated/urchin] has joined ##hplusroadmap
04:40 -!- Gurkenglas [~Gurkengla@unaffiliated/gurkenglas] has joined ##hplusroadmap
04:51 -!- sachy [~sachy@91.146.121.5] has quit [Quit: Leaving.]
04:53 -!- Urchin [~urchin@unaffiliated/urchin] has quit [Ping timeout: 272 seconds]
05:00 -!- Urchin [~urchin@unaffiliated/urchin] has joined ##hplusroadmap
06:03 -!- Jay_Dugger [~jwdugger@47.185.249.138] has joined ##hplusroadmap
07:04 < kanzure> Jay_Dugger: welcome back
07:10 < ebowden_> https://www.youtube.com/watch?v=kFFyeHJDI50
07:11 < nsh> how do i become a crackpot that has research subsidised by the US Navy plx
07:11 < nsh> sounds like decent job security
07:12 < nsh> every few months write up [have a bash script generate] some reports with copious amounts of heavy acronym sprinklings
07:14 < ebowden_> He keeps mispronouncing methodology in this video.
07:28 < kanzure> https://diamondage.com/2019/02/22/the-challenge-of-single-cell-rna-seq-and-differential-expression/
07:29 < kanzure> .tw https://twitter.com/andrewhessel/status/1099218910648258561
07:29 < yoleaux> I could watch these genetically modified T-cells kill cancer cells all day long https://cnet.co/2Gspnbr via @CNET (@andrewhessel)
07:30 < kanzure> .tw https://twitter.com/AaronJDy/status/1098669008696557575
07:30 < yoleaux> New DNA system called 'Hachimoji DNA' uses an 8-letter genetic code (hachi=eight and moji=letter in Japanese). 🧬🧬🧬 Has normal A, T, G, and C, plus new bases of B, Z, P, and S. 8-letter DNA can also be transcribed using an engineered T7 RNA polymerase. http://science.sciencemag.org/content/363/6429/884 https://pbs.twimg.com/media/Dz9ACsTX0AE0Qkh.jpg https://pbs.twimg.com/media/Dz9ADmjX0AE3Szt.jpg (@AaronJDy)
07:30 < kanzure> .title http://science.sciencemag.org/content/363/6429/884
07:30 < yoleaux> Hachimoji DNA and RNA: A genetic system with eight building blocks | Science
07:31 < kanzure> .tw https://twitter.com/Addgene/status/1098578694963109888
07:31 < yoleaux> Ready to "Evolv-R" your targeted mutagenesis approaches? By fusing an error prone polymerase with a nicking #Cas9, the @johndueber lab created a new tool for targeted mutagenesis. #Scicomm-er @ElizabethKenkel writes about this on the Addgene blog. #CRISPR https://hubs.ly/H0gJzgl0 (@Addgene)
07:31 < kanzure> https://blog.addgene.org/targeted-mutagenesis-with-evolvr
07:32 -!- sachy [~sachy@91.146.121.5] has joined ##hplusroadmap
07:36  * nsh wonders what nicking is
07:36 < nsh> .wik Nickase
07:36 < yoleaux> "A nicking enzyme (or nicking endonuclease) is an enzyme that cuts one strand of a double-stranded DNA at a specific recognition nucleotide sequences known as a restriction site. Such enzymes hydrolyse (cut) only one strand of the DNA duplex, to produce DNA molecules that are “nicked”, rather than cleaved." — https://en.wikipedia.org/wiki/Nickase
07:36 < nsh> ah, gotcha
07:42 < kanzure> nsh: you are condemned to reading http://diyhpl.us/wiki/gene-editing
07:42 < kanzure> oh i guess nickase is only mentioned once
07:44  * nsh reads regardless
07:44 < nsh> or more realistically lodges for future reading
07:49 < Jay_Dugger> Thank you, kanzure.
07:49 < kanzure> everything ok?
07:49 < Jay_Dugger> Yup. New job.
07:49 < kanzure> are you sick of cats yet?
07:49 < Jay_Dugger> Nope.
07:51 < Jay_Dugger> All been well in my absence?
07:54 < kanzure> oh, the usual.
07:56 < nsh> employment.. bleh
07:56 < nsh> i think i have to do some of that this year
07:56 < nsh> probably
08:06 -!- yashgaroth [~yashgarot@2606:6000:c308:f700:d5c7:980e:a375:194c] has joined ##hplusroadmap
08:10 < gnusha> https://secure.diyhpl.us/cgit/diyhpluswiki/commit/?id=8646b3d8 Bryan Bishop: transcript: brian bushway >> http://diyhpl.us/diyhpluswiki/transcripts/bodyhackingcon/2019/introduction/
08:16 -!- l_wl [~l_wl@pool-108-45-141-95.washdc.fios.verizon.net] has joined ##hplusroadmap
08:48 < gnusha> https://secure.diyhpl.us/cgit/diyhpluswiki/commit/?id=0a57cd0d Bryan Bishop: more words >> http://diyhpl.us/diyhpluswiki/transcripts/bodyhackingcon/2019/brian-bushway/
09:13 < kanzure> https://futurism.com/journal-retracts-ethics-article-doctor-gene-edited-babies
09:15 -!- CryptoDavid [uid14990@gateway/web/irccloud.com/x-yycaqszijcthnrnt] has joined ##hplusroadmap
09:23 < kanzure> wow, it's gone https://www.liebertpub.com/doi/10.1089/crispr.2018.0051
09:23 < kanzure> http://diyhpl.us/~bryan/papers2/bio/Draft%20ethical%20principles%20for%20therapeutic%20assisted%20reproductive%20technologies%20-%20He%20Jiankui%20-%202018.pdf
09:24 < kanzure> .tw https://twitter.com/kanzure/status/1099358383155630080
09:24 < yoleaux> Since @CRISPRjournal is now censoring #HeJiankui's pro-designer baby ethics, you can now find his paper here: http://sci-hub.tw/https://www.liebertpub.com/doi/10.1089/crispr.2018.0051 https://twitter.com/CRISPRjournal/status/1098611555346698240 (@kanzure)
09:37 < kanzure> .tw https://twitter.com/kanzure/status/1099362561164800001
09:37 < yoleaux> Academia has no leg to stand on censoring bioethics on one hand and arguing in favor of bioethics ("as long as it agrees with me") on the other. This is not scholarly at all. #notmybioethics (@kanzure, in reply to tw:1099359802327736320)
09:40 < kanzure> dcacbb2e3ab258aea0fb0af1d4a5aadef12b57986a5b10303ba9e2e87a534e45
09:53 < kanzure> https://www.genengnews.com/insights/he-jiankuis-germline-editing-ethics-article-retracted-by-the-crispr-journal/
09:58 < nsh> heh
09:59 < nsh> another strong argument against journals-as-gatekeepers
09:59 < nsh> or implicit facilitators/censors of open discussion
12:18 -!- Gurkenglas_ [~Gurkengla@unaffiliated/gurkenglas] has joined ##hplusroadmap
12:22 -!- Gurkenglas [~Gurkengla@unaffiliated/gurkenglas] has quit [Ping timeout: 268 seconds]
12:26 -!- dentist_ is now known as dentist
13:24 -!- Gurkenglas_ is now known as Gurkenglas
14:53 < kanzure> http://prophasebiostudios.org/index.php/2018/02/13/official-statement-regarding-recent-viral-media/
15:44 < nsh> pedantry: the media isn't viral, only the content
15:44 < nsh> the media can be virulent though
15:45 < nsh> very CYA statement
15:45 < nsh> hard for people to engage in genuine reasoned discussion when they're frantically covering their arses against real or imagined mobs
15:48 < nsh> "Machiavelli Davis" is a pretty solid name
17:21 -!- CryptoDavid [uid14990@gateway/web/irccloud.com/x-yycaqszijcthnrnt] has quit [Quit: Connection closed for inactivity]
18:07 -!- Urchin [~urchin@unaffiliated/urchin] has quit [Remote host closed the connection]
18:34 -!- kuldeep [~kuldeep@unaffiliated/kuldeepdhaka] has quit [Read error: Connection reset by peer]
18:34 -!- kuldeep [~kuldeep@unaffiliated/kuldeepdhaka] has joined ##hplusroadmap
19:30 -!- ebowden_ [~ebowden@unaffiliated/ebowden] has quit [Ping timeout: 250 seconds]
19:36 < superkuh> I wish people on twitter would put alternate means of contact on their profiles. Anyone care to forward, "https://people.xiph.org/~greg/decentralized-time.txt" to https://people.xiph.org/~greg/decentralized-time.txt (re: Attack-resistant decentralized time and location services via Nakamoto chain consensus.)
19:36 < superkuh> ER. https://mobile.twitter.com/midmagic/status/1099407247078084608?p=p
19:36 < superkuh> Oh, he found it. Ignore me. Bleh.
20:00 -!- Gurkenglas [~Gurkengla@unaffiliated/gurkenglas] has quit [Ping timeout: 245 seconds]
20:13 -!- ebowden [~ebowden@unaffiliated/ebowden] has joined ##hplusroadmap
20:23 -!- ebowden [~ebowden@unaffiliated/ebowden] has quit [Read error: Connection reset by peer]
20:24 -!- ebowden [~ebowden@unaffiliated/ebowden] has joined ##hplusroadmap
20:30 -!- ebowden [~ebowden@unaffiliated/ebowden] has quit [Remote host closed the connection]
20:41 -!- hehelleshin [~talinck@cpe-174-97-113-184.cinci.res.rr.com] has joined ##hplusroadmap
20:44 -!- helleshin [~talinck@cpe-174-97-113-184.cinci.res.rr.com] has quit [Ping timeout: 240 seconds]
22:16 -!- yashgaroth [~yashgarot@2606:6000:c308:f700:d5c7:980e:a375:194c] has quit [Quit: Leaving]
22:32 < nmz787> .wik ice lithography
22:32 < yoleaux> "Scanning probe lithography (SPL) describes a set of nanolithographic methods to pattern material on the nanoscale using scanning probes. It is a direct-write, mask-less approach which bypasses the diffraction limit and can reach resolutions below 10 nm." — https://en.wikipedia.org/wiki/Scanning_probe_lithography
22:33 < nmz787> "An ice resist – for example, frozen water – is particularly attractive for making nano-devices because it is easily vaporized, does not leave a residue, and can be removed without using aggressive organic solvents"
22:33 < nmz787> http://golovchenko.physics.harvard.edu/Han%20et%20al%202011.pdf
--- Log closed Sun Feb 24 00:00:58 2019