--- Log opened Mon May 20 00:00:17 2019 00:10 -!- spaceangel [~spaceange@ip-86-49-16-65.net.upcbroadband.cz] has joined ##hplusroadmap 00:17 -!- periffis [~g@162.217.45.235] has quit [Read error: Connection reset by peer] 04:57 < archels> .g DNAplotlib 04:57 < yoleaux> https://github.com/VoigtLab/dnaplotlib 05:48 -!- periffis [~g@66.205.193.3] has joined ##hplusroadmap 07:38 < kanzure> "Architecting discovery: A model for how engineers can help invent tools for neuroscience" https://www.cell.com/neuron/fulltext/S0896-6273(19)30286-7 07:38 < kanzure> .tw https://twitter.com/DARPA/status/1129439531147644928 07:38 < yoleaux> Know what's cool? This: https://go.usa.gov/xmdWE. We plan to fund a "Direct to Phase 2" #SBIR project to design, fabricate & clinically demonstrate a wearable, conformable light delivery device for use in conjunction with optogenetic transgene applications. #optogenetics https://pbs.twimg.com/media/D6yTK5IXYAIUQfg.jpg (@DARPA) 07:39 -!- uniera [uniera@gateway/vpn/privateinternetaccess/uniera] has joined ##hplusroadmap 07:39 < kanzure> "Ordered insertional mutagenesis at a single genomic site enables lineage tracing and analog recording in mammalian cells" https://www.biorxiv.org/content/10.1101/639120v1 https://twitter.com/brian_tenner/status/1129282403862605824 07:40 < kanzure> .tw https://twitter.com/AmandaAskell/status/1129096387382038528 07:40 < yoleaux> A few months I took part in a panel on "What Goal Should Civilization Strive For?" at the Beneficial AGI conference in Puerto Rico. tl;dr: no one knows, so we should probably gather evidence and take incrementally good steps that preserve option value. https://www.youtube.com/watch?v=J_X--IkOeAg&t=1s (@AmandaAskell) 07:41 < kanzure> "De novo design of tunable, pH-driven conformational changes" https://science.sciencemag.org/content/364/6441/658 https://twitter.com/UWproteindesign/status/1129086306003197952 07:42 < kanzure> "We present a general strategy to design pH-responsive protein conformational changes by precisely preorganizing histidine residues in buried hydrogen-bond networks. We design homotrimers and heterodimers that are stable above pH 6.5 but undergo cooperative, large-scale conformational changes when the pH is lowered and electrostatic and steric repulsion builds up as the network histidine ... 07:42 < kanzure> ...residues become protonated. The transition pH and cooperativity can be controlled through the number of histidine-containing networks and the strength of the surrounding hydrophobic interactions." 07:43 < kanzure> https://chanzuckerberg.com/rfa/essential-open-source-software-for-science/ https://twitter.com/cziscience/status/1128693937130991623 07:44 < kanzure> .tw https://twitter.com/neuroecology/status/1128658309429518336 07:44 < yoleaux> @MatteoCarandini Back when I was a real person I wrote some blog posts about the retina receiving inputs from the rest of the brain ("retinopetal") and why it might want to do that https://neuroecology.wordpress.com/2017/03/29/the-retina-receives-signals-from-all-over-the-brain-and-that-is-kind-of-weird/ https://neuroecology.wordpress.com/2017/08/09/why-does-the-eye-care-about-the-nose/ (@neuroecology, in reply to tw:1128657806150832129 07:44 < yoleaux> ) 07:46 < kanzure> "Acoustically detonated biomolecules for genetically encodable inertial cavitation" https://www.biorxiv.org/content/10.1101/620567v1 https://twitter.com/mikhailshapiro/status/1122902970046091264 07:47 < kanzure> https://spikesphotons.blog/2019/05/13/using-rabies-virus-for-tracing-neural-connections-caveats-and-limitations-exposed-by-studies-of-barrel-cortex-circuits/ 07:48 < kanzure> "Recording temporal data onto DNA with minutes resolution" https://www.biorxiv.org/content/10.1101/634790v1 https://twitter.com/brian_tenner/status/1127802313345658882 07:49 < kanzure> "We show that we can determine the time of Co2+ addition to within two minutes over a 60-minute period. Further, we can estimate the rise and fall of an input Co2+ pulse to within five minutes. In silico simulations run based on our in vitro data predict TURTLES can record pulses as short as 1 minute and could record up to 10 pulses with high accuracy. TURTLES has 200-fold better temporal ... 07:49 < kanzure> ...resolution than other DNA-based recording techniques." 07:49 < kanzure> i think our approach is better? wow. 07:56 -!- uniera_ [~uniera@a109-50-141-72.cpe.netcabo.pt] has joined ##hplusroadmap 07:58 -!- uniera [uniera@gateway/vpn/privateinternetaccess/uniera] has quit [Ping timeout: 245 seconds] 07:58 < kanzure> archels: twitter seems to be recommending me exclusively human brain project accounts now 07:58 < kanzure> i assume this is your doing. 08:00 < kanzure> egan stuff http://ttapress.com/CrystalNights.pdf 08:00 < kanzure> .title https://quantum.country/qcvc 08:00 < yoleaux> Quantum computing for the very curious 08:01 < kanzure> "De-novo discovery of tumoricidal aptamers in a DNA sequencing chip" https://www.biorxiv.org/content/10.1101/630830v1 08:01 < kanzure> .tw https://twitter.com/adammarblestone 08:01 < yoleaux> Och, A cannae finde nae tweet 08:01 < kanzure> whoops 08:01 < kanzure> .tw https://twitter.com/DrTomEllis/status/1126771904822407168 08:01 < yoleaux> Nice idea in this preprint - sequence a DNA library on NextSeq, and then load tumour cells onto the flow cell and see which DNA aptamers are tumoricidal > Sequencers can done more than just sequence! https://www.biorxiv.org/content/10.1101/630830v1 (@DrTomEllis) 08:05 -!- uniera_ [~uniera@a109-50-141-72.cpe.netcabo.pt] has quit [Quit: Leaving...] 08:05 < kanzure> "Population imaging of neural activity in awake behaving mice in multiple brain regions" https://www.biorxiv.org/content/10.1101/616094v1 https://twitter.com/eboyden3/status/1121778597205827584 08:06 < kanzure> .tw https://twitter.com/Ginger_Skinner/status/1121045280613388288 08:06 < yoleaux> A team at Harvard’s @wyssinstitute has recorded the lineage in every cell in a mouse’s body. Here’s a cool look at what the new technique means for unlocking the mechanisms of human development, #aging, and disease. https://endpoints.elysiumhealth.com/scannable-barcodes-and-role-of-cell-lineage-in-human-disease-and-aging-7f5bd3ded145 @ElysiumHQ (@Ginger_Skinner) 08:23 -!- l_wl [~l_wl@pool-173-66-183-216.washdc.fios.verizon.net] has quit [Remote host closed the connection] 08:23 -!- l_wl [~l_wl@pool-173-66-183-216.washdc.fios.verizon.net] has joined ##hplusroadmap 09:10 -!- strages [uid11297@gateway/web/irccloud.com/x-inbhnjvpzdjiqxyo] has joined ##hplusroadmap 09:21 < archels> kanzure: but do those HBP accounts actually post interesting stuff? 09:33 < kanzure> i don't think so? 09:33 < kanzure> haven't checked 09:34 -!- yashgaroth [~ffffffff@2606:6000:ca81:3100:9462:157:c20f:5cf9] has joined ##hplusroadmap 09:58 -!- NikopolSohru [~Nikopol_@5.79.76.37] has joined ##hplusroadmap 09:59 -!- NikopolSohru [~Nikopol_@5.79.76.37] has quit [Changing host] 09:59 -!- NikopolSohru [~Nikopol_@unaffiliated/nikopolsohru] has joined ##hplusroadmap 10:30 < archels> sorry, working on it 11:28 < heath> le sigh: https://azelef.github.io/scihub/index.html 11:28 < heath> Sci-hub domain checker...no currently fully functioning domains 11:45 -!- chao [~chao@2601:647:5200:52df:a036:ef2:1639:f0e9] has joined ##hplusroadmap 11:49 -!- Urchin [~urchin@unaffiliated/urchin] has quit [Remote host closed the connection] 11:51 -!- yashgaroth [~ffffffff@2606:6000:ca81:3100:9462:157:c20f:5cf9] has quit [Read error: Connection reset by peer] 11:52 -!- yashgaroth [~ffffffff@cpe-76-176-31-235.san.res.rr.com] has joined ##hplusroadmap 12:01 < kanzure> heath: https://sci-hub.tw/ 12:07 -!- LooCfur [~hello@loocfur.powered.by.lunarbnc.net] has quit [Remote host closed the connection] 13:05 -!- periffis [~g@66.205.193.3] has quit [Quit: periffis] 13:48 -!- NikopolSohru [~Nikopol_@unaffiliated/nikopolsohru] has quit [Quit: Leaving] 14:23 < nmz787> heath: just check wikipedia, they always seem to list a server that's up 15:10 -!- NikopolSohru [~Nikopol_@5.79.76.37] has joined ##hplusroadmap 15:10 -!- NikopolSohru [~Nikopol_@5.79.76.37] has quit [Changing host] 15:10 -!- NikopolSohru [~Nikopol_@unaffiliated/nikopolsohru] has joined ##hplusroadmap 15:11 -!- LeoTal [~Adium@72.170.86.79.rev.sfr.net] has quit [Quit: Leaving.] 15:21 -!- NikopolSohru [~Nikopol_@unaffiliated/nikopolsohru] has quit [Quit: Leaving] 15:34 -!- spaceangel [~spaceange@ip-86-49-16-65.net.upcbroadband.cz] has quit [Remote host closed the connection] 16:03 -!- TC [~talinck@98.29.27.253] has joined ##hplusroadmap 16:06 -!- chao [~chao@2601:647:5200:52df:a036:ef2:1639:f0e9] has quit [] 16:06 -!- hehelleshin [~talinck@98.29.27.253] has quit [Ping timeout: 246 seconds] 17:04 -!- purpleshift [purpleshif@gateway/vpn/mullvad/purpleshift] has joined ##hplusroadmap 18:18 -!- periffis [~g@66.205.193.3] has joined ##hplusroadmap 18:20 -!- strages [uid11297@gateway/web/irccloud.com/x-inbhnjvpzdjiqxyo] has quit [Quit: Connection closed for inactivity] 19:27 -!- purpleshift [purpleshif@gateway/vpn/mullvad/purpleshift] has quit [Quit: .] 20:02 -!- LeoTal [~Adium@72.170.86.79.rev.sfr.net] has joined ##hplusroadmap 20:17 -!- periffis [~g@66.205.193.3] has quit [Quit: periffis] 21:25 -!- LooCfur [~hello@loocfur.powered.by.lunarbnc.net] has joined ##hplusroadmap 22:11 -!- l_wl_ [~l_wl@pool-173-66-183-216.washdc.fios.verizon.net] has joined ##hplusroadmap 22:14 -!- l_wl [~l_wl@pool-173-66-183-216.washdc.fios.verizon.net] has quit [Ping timeout: 259 seconds] 22:42 -!- yashgaroth [~ffffffff@cpe-76-176-31-235.san.res.rr.com] has quit [Quit: Leaving] --- Log closed Tue May 21 00:00:18 2019