--- Log opened Fri Jun 26 00:00:01 2020
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05:29 < kanzure> looks like there's a lot of works derivative of "De novo DNA synthesis using polymerase-nucleotide conjugates" since i last looked: https://scholar.google.com/scholar?cites=13477935498039776225&as_sdt=5,44&sciodt=0,44&hl=en
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06:23 < gnusha> https://secure.diyhpl.us/cgit/diyhpluswiki/commit/?id=3ec51f2b Michael Folkson: Add Tim Ruffing on Schnorr multisig at London Bitcoin Devs >> http://diyhpl.us/diyhpluswiki/transcripts/london-bitcoin-devs/2020-06-17-tim-ruffing-schnorr-multisig/
06:23 < gnusha> https://secure.diyhpl.us/cgit/diyhpluswiki/commit/?id=fce09b85 Bryan Bishop: Merge pull request #121 from michaelfolkson/add-tim-ruffing-multisig >> http://diyhpl.us/diyhpluswiki/
06:26 < kanzure> .tw https://twitter.com/kanzure/status/1276507065947742209
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06:26 < saxo> Transcript: Tim Ruffing @real_or_random on "Taproot and Schnorr multisignatures" https://diyhpl.us/wiki/transcripts/london-bitcoin-devs/2020-06-17-tim-ruffing-schnorr-multisig/ @LDNBitcoinDevs @pwuille @Blockstream ty @michaelfolkson && @btctranscripts (@kanzure)
06:29 < kanzure> iGEM mentorship application form https://2020.igem.org/Teams/Mentorship - in case any of you want to serve as a mentor to some iGEM teams...
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06:41 < kanzure> .tw https://twitter.com/balajis/status/1276009719933489152
06:41 < saxo> We haven't made the emotional case for technology. // The assumption behind sci-fi like Black Mirror is that the present is ok, but tech may make the future dystopian. // But perhaps the present is dystopian and tech is our only hope for a positive future. (@balajis)
06:41 < kanzure> .tw https://twitter.com/micsolana/status/1276273167728652289
06:41 < saxo> a lot of you seem to have forgotten the goal is immortality (@micsolana)
06:41 < kanzure> .tw https://twitter.com/micsolana/status/1276344377363755009
06:41 < saxo> ppl keep yelling at me about kids. "why do you think we have children?" um, because they say funny things and have cute little googly eyes? love kids, they should be immortal too. next stupid question. https://twitter.com/micsolana/status/1276273167728652289 (@micsolana)
06:43 < kanzure> .title https://github.com/google/skywater-pdk
06:43 < saxo> GitHub - google/skywater-pdk: Open source process design kit for usage with SkyWater Technology Foundry's 130nm node.
06:43 < kanzure> "The SkyWater Open Source PDK is a collaboration between Google and SkyWater Technology Foundry to provide a fully open source Process Design Kit and related resources, which can be used to create manufacturable designs at SkyWater’s facility." [at 130nm node capability]
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06:44 < kanzure> "things that people have done with 130nm" https://docs.google.com/document/d/1sMmoCfS5l6Uz8sl9Bk3R32XtsdZmC1Hw6bY9nm6r-PA/edit
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06:46 < kanzure> https://hackaday.com/2020/06/25/creating-a-custom-asic-with-the-first-open-source-pdk/
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06:58 < kanzure> "Super-resolution localization microscopy: Toward high throughput, high quality, and low cost" https://aip.scitation.org/doi/10.1063/5.0011731
07:00 < kanzure> .tw https://twitter.com/bradtheilman/status/1276166948682731520
07:00 < saxo> An important distinction I see myself and others sometimes forget: // We never *measure* firing rates. We *estimate* them, usually by counting spikes. // Rates aren't what exists, only the spikes exist. (@bradtheilman)
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09:13 < kanzure> ok, here's my mistake, sequencing by synthesis does not involve a single fluorophore observation, it's an aggregated fluorescence observation from many dna molecules getting sequenced in parallel with the assumption being that the polymerases are all in lockstep
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09:39 < mrdata> quite an assumption
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09:42 < kanzure> well that's why the read lengths are short in sequencing-by-synthesis
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11:02 < yashgaroth> ah nm it is the chemistry getting out of sync rather than the polymerase, again very similar to pamidite synthesis...apparently it's called a phasing error for illumina's tech
11:11 < kanzure> oh.
11:12 < kanzure> sounds like monitoring for fluorescent incorporation events may not materially improve the terminator-based synthesis strategy
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13:03 < yashgaroth> something closer to pacbio's method would be a better fit for single-molecule synthesis, but their tech relies more on physics than biology...and, again, breaking past 99% detection efficiency would be tough as pacbio's accuracy is notoriously trash
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18:20 < fenn> pacbio does single molecule sequencing via fluorophore observation
18:20 < fenn> it was a huge imaging hardware effort to get the signal to noise ratio to be adequate
18:21 < fenn> probably easier to do if you're only writing one molecule instead of an entire plate of microwells
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--- Log closed Sat Jun 27 00:00:02 2020