--- Log opened Tue Jul 27 00:00:20 2021 00:49 -!- CryptoDavid [uid14990@id-14990.highgate.irccloud.com] has quit [Quit: Connection closed for inactivity] 01:10 -!- livestradamus [~quassel@user/livestradamus] has quit [Quit: https://quassel-irc.org - Chat comfortably. Anywhere.] 01:50 -!- HumanG33k [~HumanG33k@dau94-2-82-66-65-160.fbx.proxad.net] has quit [Ping timeout: 258 seconds] 02:57 -!- HumanG33k [~HumanG33k@2a01:e0a:95:5d90:215:c5ff:fe68:fb04] has joined #hplusroadmap 05:31 -!- Croran [~quassel@2601:601:1880:7780:586:f4af:c62b:6c92] has quit [Ping timeout: 256 seconds] 05:34 -!- Croran [~quassel@c-73-118-179-18.hsd1.wa.comcast.net] has joined #hplusroadmap 07:29 < gnusha> https://secure.diyhpl.us/cgit/diyhpluswiki/commit/?id=350f2cc2 Michael Folkson: Minor typo >> http://diyhpl.us/diyhpluswiki/transcripts/sydney-bitcoin-meetup/2021-06-01-socratic-seminar/ 07:29 < gnusha> https://secure.diyhpl.us/cgit/diyhpluswiki/commit/?id=1aa0425a Michael Folkson: Merge pull request #225 from michaelfolkson/sydney-typo >> http://diyhpl.us/diyhpluswiki/ 07:29 < gnusha> https://secure.diyhpl.us/cgit/diyhpluswiki/commit/?id=47313032 Michael Folkson: Add July Sydney Socratic >> http://diyhpl.us/diyhpluswiki/transcripts/sydney-bitcoin-meetup/2021-07-06-socratic-seminar/ 07:29 < gnusha> https://secure.diyhpl.us/cgit/diyhpluswiki/commit/?id=3f297cc6 Michael Folkson: Merge pull request #226 from michaelfolkson/add-sydney-july >> http://diyhpl.us/diyhpluswiki/ 13:07 < nmz787> .title https://en.wikipedia.org/wiki/Hoogsteen_base_pair 13:07 < saxo> "A Hoogsteen base pair is a variation of base-pairing in nucleic acids such as the A•T pair. In this manner, two nucleobases, one on each strand, can be held together by hydrogen bonds in the major groove." - https://en.wikipedia.org/wiki/Hoogsteen_base_pair 13:09 < nmz787> .wik g quadruplex 13:09 < saxo> "In molecular biology, G-quadruplex secondary structures (G4) are formed in nucleic acids by sequences that are rich in guanine. They are helical in shape and contain guanine tetrads that can form from one, two or four strands. The unimolecular forms often occur naturally [...]" - https://en.wikipedia.org/wiki/G_quadruplex 13:10 < nmz787> .wik triple stranded DNA 13:10 < saxo> Article not found: https://en.wikipedia.org/wiki/Triple_stranded_DNA gave 404 | Searched en for 'triple stranded DNA' | https://en.wikipedia.org/wiki/No_result_found gave 404 | Searched en for 'No result found' 13:10 < nmz787> .title https://en.wikipedia.org/wiki/Triple-stranded_DNA 13:10 < saxo> "Triple-stranded DNA (also known as H-DNA or Triplex-DNA) is a DNA structure in which three oligonucleotides wind around each other and form a triple helix." - https://en.wikipedia.org/wiki/Triple-stranded_DNA 13:13 < kanzure> wait until he learns about dendromers 13:13 < kanzure> dendrimers? 13:22 -!- chiastre [~chiastre@2a02:8388:8003:c180:77a:699e:3ef5:d0a4] has quit [Ping timeout: 240 seconds] 13:23 -!- HumanG33k [~HumanG33k@2a01:e0a:95:5d90:215:c5ff:fe68:fb04] has quit [Ping timeout: 268 seconds] 13:25 -!- chiastre [~chiastre@user/chiastre] has joined #hplusroadmap 13:28 -!- HumanG33k [~HumanG33k@2a01:e0a:95:5d90:215:c5ff:fe68:fb04] has joined #hplusroadmap 13:59 < lsneff> Do any of you remember that weird electrochemical 3d printer that used a grid of electrodes to print surprisingly quickly? 15:06 < nmz787> .title https://www.sciencedirect.com/science/article/pii/S0076687920301816 15:06 < saxo> Emulsion-based directed evolution of enzymes and proteins in yeast - ScienceDirect 15:07 < nmz787> basically, clone your gene library upstream of a thermostable polymerase, and if your gene is induced the polymerase gets generated, then you can just do colony PCR (without adding exogenous polymerase with the PCR mastermix) and if it amplifies, you know the gene was a match to your induction molecule 15:07 < nmz787> in that case, they were trying to find GPCRs 16:47 < lsneff> Found it https://fabric8labs.com/ 17:13 < jrayhawk> oh wow, that is a neat idea 17:13 < jrayhawk> .title https://www.youtube.com/watch?v=d60llr9g-y4 17:13 < saxo> T3 Pitch: Fabric 8 Labs - YouTube 18:05 < lsneff> @jrayhawk I'm not able to watch that video atm, does it talk more about how it works? 18:08 < lsneff> Okay, watched a bit of it with captions. Seems like the magic part is the electrode array and the chemistry work they've put into it 18:09 < lsneff> The annoying thing is that they'll definitely patent this (or probably already have), and stop anyone else from using this obviously better and cheaper way to manufacture things 18:25 < L29Ah> tl;dw: electroplating with an electrode array 18:25 < jrayhawk> they're glossing over a lot of basic stuff regarding commercial viability, most notably deposition speed at current scale, and how bad that deposition speed is going to get at higher footprints 18:26 < jrayhawk> and what the cost of the cathode base per print is 18:34 < jrayhawk> it looks like a win from a safety perspective; though. metal dust sintering and lithography are both pretty scary relative to a simple metal solute 18:41 < lsneff> The cathode base isn't anything fancy, right? Just a sheet of metal 18:41 < jrayhawk> depends; do they need to flow solute through it to get even deposition at higher footprints? 18:44 < lsneff> Good question 18:45 < lsneff> Are they using the tft backplane as an electrode grid without the actual lcd part on that? 18:45 < jrayhawk> yeah 18:48 < lsneff> Is that something that is available on the market? 18:49 < lsneff> Found the patent. So, this technology isn't going to be available from anyone except this company or other company they license the patent to until 2036 18:49 < lsneff> That's stupid 18:51 < abetusk> https://www.protocols.io/view/one-hour-covid-test-protocol-bktakwie.html 18:51 < abetusk> apologies if it's already been shown here 18:51 < jrayhawk> they're interested in retailing the product, so it is available to everyone at a price 18:56 < lsneff> It's dumb that they can patent something this fundamental. If they patented a particular anode design or something, I'd get it, but the patent is extremely broad. 18:57 < fenn> "each printer consumes $2.5 million worth of consumables over its life" (as if that were a good thing) 18:57 < lsneff> It basically covers anything that uses electrochemical processes on a grid to make a 3d object 19:00 < fenn> i must have missed the part where they explained how it works 19:01 < fenn> lsneff wanna go to mars? 19:01 < lsneff> I wouldn't say no. Why? 19:02 < fenn> so we can do whatever we want and there aren't any dudes with guns telling us no 19:03 < fenn> it won't be until 2036 but i'm certain there will be many more such oppressive developments in the future 19:04 < lsneff> I'm very sure there will be. 19:05 < fenn> it's not the kind of space pirate i was imagining 19:05 < lsneff> This is the much more interesting kind 19:07 < lsneff> I'm legitimately worried that 3D printing and automated manufacturing isn't advancing fast enough to make a civilization seed possible before people get to Mars 19:09 < lsneff> I know it's a dumb thing to be worried about 19:19 < L29Ah> having a submitted patent doesn't necessarily mean that you're going to be persecuted for doing things written in it 19:19 < L29Ah> all kinds of stupidly broad patents exist 19:20 < L29Ah> and i guess you could find a dozen of publications of the idea involved dated well before the patent in your defense, as it's not rocket science 19:22 < lsneff> That sounds like something I'd need lawyers to sort out, say if I went and made an open-source implementation of this technology. 19:22 < L29Ah> 05:02:20] so we can do whatever we want and there aren't any dudes with guns telling us no 19:22 < L29Ah> what about guys with kinetic penetrators? mars transfer orbital speed is no joke 19:22 < L29Ah> i don't recall any patents preventing open source algorithms popping up, not sure about diy hardware designs tho 19:23 < lsneff> I think software patents are a lot harder to enforce 19:24 < lsneff> Hardware patents definitely slowed down the commodification of 3D printing 19:38 < lsneff> Oh also, if y'all remember the microfluidic discussion a few months ago: a TFT backplane could be used to implement that without weird optofluidics 19:39 < fenn> i think the voltage is too low 19:39 < fenn> you need tens of volts 19:40 < fenn> worth a shot though 19:40 < fenn> if making your own chips, sure 21:50 -!- mrdata [~mrdata@user/mrdata] has quit [Ping timeout: 268 seconds] --- Log closed Wed Jul 28 00:00:21 2021