--- Log opened Thu Aug 08 00:00:08 2024
--- Day changed Thu Aug 08 2024
00:00 < hprmbridge> nmz787> and they are skeptical of obedience
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10:07 < hprmbridge> kanzure> "Single cortical neurons as deep artificial neural networks" https://pubmed.ncbi.nlm.nih.gov/34380016/
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13:10 < hprmbridge> fodagut> fenn: https://www.lesswrong.com/posts/rQxrSRPJGmksGjK8z/it-s-time-for-a-self-reproducing-machine
13:16 < hprmbridge> kanzure> ah, carlf
13:16 < hprmbridge> kanzure> a real feynman but he has internet access
13:16 < hprmbridge> kanzure> h he's been posting more recently
13:17 < hprmbridge> kanzure> I wonder if he would be satisfied with a biological cell for his answer
13:18 < hprmbridge> kanzure> someone should show him https://diyhpl.us/wiki/transcripts/reprap/brian-minnick-2021/
13:21 < RangerMauve> Whoa, is that legit? How big a scale have those gotten to now?
13:24 < hprmbridge> kanzure> @fodagut I don't want to post on the Wr*my site at the moment, but feel free to pass along https://x.com/kanzure/status/1821643399956431010
13:28 < RangerMauve> https://clickprintchem.wordpress.com/2022/05/31/self-replicating-3d-printer-year-3-and-4-review/
13:28 < RangerMauve> neat
13:32 < RangerMauve> surprising there's no timelapse of a full print
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15:00 < hprmbridge> kanzure> okay replied here https://x.com/carl_feynman/status/1821662752584200527
15:10 < L29Ah> .t
15:10 < saxo> x.com
15:19 < hprmbridge> kanzure> https://projectquine.substack.com/
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16:05 < hprmbridge> alonzoc> Clanking replicators are usually forgotten when machine self-replication is discussed the real issue tho is making the needed integrated circuits. Extending the autofac to IC production would add a fair bit of additional complexity
16:07 < hprmbridge> alonzoc> Really cool to see the 3d printing of PCBs tho I had a look at the problem a while back and was considering just giving a 3d printer a milling attachment
16:34 < hprmbridge> kanzure> "A self-replicating peptide" https://www.nature.com/articles/382525a0 (1996)
16:34 < hprmbridge> kanzure> "The production of amino acids and their condensation to polypeptides under plausibly prebiotic conditions have long been known1,2. But despite the central importance of molecular self-replication in the origin of life, the feasibility of peptide self-replication has not been established experimentally3–6. Here we report an example of a self-replicating peptide. We show that a 32-residue α-helical
16:34 < hprmbridge> kanzure> peptide based on the leucine-zipper domain of the yeast transcription factor GCN4 can act autocatalytically in templating its own synthesis by accelerating the thioester-promoted amide-bond condensation of 15- and 17-residue fragments in neutral, dilute aqueous solutions. The self-replication process displays parabolic growth pattern with the initial rates of product formation correlating with the
16:34 < hprmbridge> kanzure> square-root of initial template concentration."
16:36 < hprmbridge> kanzure> "Self-replication of complementary nucleotide-based oligomers" https://www.nature.com/articles/369221a0 (1994)
16:37 < hprmbridge> kanzure> "Natural replication of nucleic acids, however, utilizes complementary rather than self-complementary strands. Here we report on a minimal implementation of this type of replication17 based on cross-catalytic template-directed syntheses of hexadeoxy nucleotide derivatives from amino-trideoxynucleotides. In our experiments, two self-complementary and two complementary templates compete for their
16:37 < hprmbridge> kanzure> combinatorial synthesis from four common trimeric precursors. We provide kinetic evidence that cross-catalytic self-replication of complementary templates can proceed with an efficiency similar to that of autocatalytic self-replication of self-complementary templates. We observe selective stimulation of template synthesis, and thus information transfer, on seeding the reaction mixtures with one of
16:37 < hprmbridge> kanzure> four chemically labelled templates bearing the sequence of the reaction products."
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17:20 < hprmbridge> kanzure> https://youtu.be/gNRnrn5DE58?si=NMk1BAsU6_XMuLbL
17:20 < hprmbridge> kanzure> https://youtu.be/djB9oK6pkbA?si=ObFPCZkR-Rx9AC2G
17:20 < hprmbridge> kanzure> "the origins of mechanical precision" etc
17:26 < hprmbridge> alonzoc> I remember watching a BBC documentary that covered the modern lathe, and how the degree of precision it allowed was one of the main blockers of the industrial revolution
17:35 < docl> have you looked up gingery's lathe stuff?
17:35 < docl> have been told it's pronounced with both g's soft as in green
17:37 < hprmbridge> kanzure> that's fenn's specialty
17:37 < hprmbridge> alonzoc> Oh I have the pdf copies of the build your own metal working shop from scratch books
17:38 < hprmbridge> alonzoc> they're really good reads. Defo recommend, they're all on libgen as well
17:38 < docl> one design I saw was a concrete bed with holes in it. the concrete shrinks as it sets, so then you pack it with nonshrink grout to add the ways
17:39 < docl> will snag, haven't actually read them yet
17:39 < docl> http://opensourcemachinetools.org/concrete-lathe/
17:39 < docl> .t
17:39 < saxo> Concrete Lathe - My CMS
17:40 < docl> the ways need to be prefectly straight. for that you can use the three rounds grinding method.
17:41 < hprmbridge> alonzoc> Beautiful. Reminds me of the lathe I learnt on which was this horrid green behemouth from the 50s that never died
17:41 < hprmbridge> alonzoc> wiki link is dead though
17:42 < docl> another cheap sounding method I've heard (from my time rooming with dani eder the boeing guy with the wiki books) is you can shine a laser pointer at a ball bearing and align the reflected cone
17:43 < docl> .t https://en.wikibooks.org/wiki/Seed_Factories
17:43 < saxo> Better Worlds I: - Wikibooks, open books for an open world
17:43 < docl> this is the relevant one for self rep, although his space systems one has some useful refs as well
17:44 < docl> seed factories are slightly adjacent, basically process decentralization / scale-downs, so you can have a network that is self rep capable
17:46 < docl> he's a good person to follow on reddit https://www.reddit.com/user/danielravennest/
17:47 < hprmbridge> alonzoc> Yeah a more fault-tolerant and distributed variant of the already existing manufacturing economy. Very early bitcoin vibes from what i'm reading
17:49 < hprmbridge> alonzoc> https://en.wikibooks.org/wiki/Seed_Factories/WWF
17:52 < hprmbridge> alonzoc> god wikibooks is a rabbit hole
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18:09 < hprmbridge> kanzure> https://www.spaceforge.com/
18:46 < hprmbridge> kanzure> "Acoustically targeted measurement of transgene expression in the brain" https://www.science.org/doi/10.1126/sciadv.adj7686
18:46 < hprmbridge> kanzure> "How could we check if a gene therapy reached the desired brain region? Here, we noninvasively measure gene delivery and endogenous gene expression in specific brain sites with ultrasound-released synthetic serum markers."
19:00 < hprmbridge> kanzure> https://en.wikipedia.org/wiki/Spiegelman%27s_Monster
19:06 < hprmbridge> kanzure> "Evidence for de novo production of self-replicating and environmentally adapted RNA structures by bacteriophage Qbeta replicase" https://epub.uni-regensburg.de/22926/1/sumper45.pdf
19:06 < hprmbridge> kanzure> (1975)
19:12 < hprmbridge> kanzure> "N.BspD6I DNA nickase strongly stimulates template-independent synthesis of non-palindromic repetitive DNA by Bst DNA polymerase" https://www.degruyter.com/document/doi/10.1515/BC.2007.043/html
19:13 < hprmbridge> kanzure> but this one seems to claim it's contamination https://www.sciencedirect.com/science/article/abs/pii/002228369190729P
19:17 < hprmbridge> kanzure> hmm this one claims it's not contamination https://www.nature.com/articles/321089a0
19:22 < hprmbridge> kanzure> reminds me of the thermus thermophilus polymerase paper
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--- Log closed Fri Aug 09 00:00:17 2024