--- Log opened Mon Sep 02 00:00:40 2024
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01:12 < hprmbridge> nmz787> Fenn wasn't that one of the original methods before controlled pore glass? Or maybe I'm thinking flat glass. I haven't read the paper yet... Are they flowing the glass beads around some analytics?
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08:06 < kanzure> "Phage-triggered reverse transcription assembles a toxic repetitive gene from a noncoding RNA" https://www.science.org/doi/10.1126/science.adq3977 https://x.com/maxewilkinson/status/1829245305193410737
08:06 < kanzure> (mentioned the other day)
08:07 < kanzure> there is also this one:
08:07 < kanzure> "De novo gene synthesis by an antiviral reverse transcriptase" https://www.biorxiv.org/content/10.1101/2024.05.08.593200v1.full
08:09 < kanzure> maybe zhang lab got scooped?
08:12 < kanzure> SOLUTATIONS: hello
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09:33 < kanzure> validation of microscopy-by-sequencing by testing against DNA origami constructs https://www.biorxiv.org/content/10.1101/2020.08.27.271072v1.abstract
09:43 < kanzure> directed evolution via in vitro compartmentalized self-selection with "molecular programs": https://pubs.acs.org/doi/abs/10.1021/acssynbio.3c00385
09:45 < kanzure> "autonomous selection" neat
09:47 < kanzure> i guess we had some of that with the phage continuous evolution stuff
09:53 < kanzure> visible light responsive azobenzene https://pubs.acs.org/doi/full/10.1021/acs.joc.2c01777 and a more general review of recent progress in azobenzenes https://www.nature.com/articles/s41570-021-00334-w
10:10 < kanzure> controlled reverse strand synthesis for synthetic DNA error correction https://api.repository.cam.ac.uk/server/api/core/bitstreams/2032f9e6-c047-4811-8f4e-8018a8363754/content
10:13 < kanzure> why synthesize with one nucleotide when you can use three? https://pubs.rsc.org/en/content/articlelanding/2023/cc/d3cc04933g "Artificial nucleotide codons for enzymatic DNA synthesis"
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10:22 < kanzure> see https://chemrxiv.org/engage/api-gateway/chemrxiv/assets/orp/resource/item/651532550065940912077718/original/artificial-nucleotide-codons-for-enzymatic-dna-synthesis.pdf
10:33 < kanzure> inkjet + TdT-based enzymatic DNA synthesis https://www.science.org/doi/full/10.1126/sciadv.adi0263
10:46 < kanzure> "Excitability and synaptic transmission after vitrification of mouse corticohippocampal slices" https://www.biorxiv.org/content/10.1101/2024.06.03.597218v1.abstract
10:54 < SOLUTATIONS> What do they mean by vitrification all I could find was glass
10:58 < kanzure> https://en.wikipedia.org/wiki/Cryonics
10:59 < kanzure> you can transform the material into a more stable format through cryovitrification
10:59 < kanzure> ideally without crystal formtaion
10:59 < kanzure> *formation
11:04 < docl> I keep thinking thermal printers might be really useful for microfluidic chemistry if you use them right
11:05 < docl> that's how they print receipts, and there are now little sticker/label/business card printers designed for a cell phone
12:00 -!- DNS is now known as BA_Dragon
12:06 < hprmbridge> .maybeman> How did i land here
12:06 < hprmbridge> .maybeman> I cant even compute a jacobian
12:09 < hprmbridge> .maybeman> Omg much quality such information
12:10 < SOLUTATIONS> Man I don’t know Latin… so what
12:11 < hprmbridge> .maybeman> Where does this bot scrape this stuff from
12:11 < hprmbridge> .maybeman> Is it an agent network
12:11 < SOLUTATIONS> I see freezing, so cryogenesis 
12:12 < SOLUTATIONS> Anyways
12:12 < SOLUTATIONS> Are you? hprmbridge 
12:16 < hprmbridge> .maybeman> Yall dont want to die huh? https://cdn.discordapp.com/attachments/1064664282450628710/1280244891804242111/IMG_5801.png?ex=66d76078&is=66d60ef8&hm=1e035ca9dbb2eddb352bb385b9e6286ca501e11aebc67d8e9db715e07a0b9564&
12:16 < hprmbridge> .maybeman> Are there any self-replicating machine progress?
12:16 < hprmbridge> .maybeman> I can imagine it to be of much importance as a future space faring civilization
12:17 < SOLUTATIONS> My specialty is do it yourself biohacking
12:17 < SOLUTATIONS> And the philosophy of science
12:20 < hprmbridge> kanzure> @.maybeman here is a recent thread on self-replication https://x.com/kanzure/status/1821665277270020316
12:21 < SOLUTATIONS> Order in the court
12:21 < SOLUTATIONS> Self replication is the beginning of life, a metaphor for where it started
13:03 < hprmbridge> nmz787> Solutations: vitrification means freezing or solidification without crystallization...
13:13 < cslr> are here people who have EEG-hardware?
13:14 < cslr> I have created computer software using EEG measurements.
13:16 < hprmbridge> kanzure>  https://cdn.discordapp.com/attachments/1064664282450628710/1280260020675678321/image0.jpg?ex=66d76e8f&is=66d61d0f&hm=d5fb6d49fb13d11b1465fd1f5f167b9ae2a2e83bc6e48d134c80627057a8c45c&
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13:22 < docl> the term vitrification is also used for high temp glassification (of industrial waste for example). but in biology it's usually going to mean low temp solidification of a water mixture without freezing (mixtures always have a lowered freezing point)
13:22 < hprmbridge> nmz787> Yes I guess I should have said solidification without crystallization, regardless of temperature
13:23 < hprmbridge> nmz787> Kanzure I've had some of those tiny motors in my parts stock for several years now
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13:24 < docl> where by freezing we technically mean "ice crystals form" (so in cryonics people will correct you and say it's not freezing). you can tell because when water forms ice crystals it releases some heat in the process
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13:39 < kanzure> "Three-dimensional nanofabrication by photon beam patterning of shrinking hydrogels" https://patents.google.com/patent/US11214661B2/en
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21:46 < hprmbridge> kanzure> https://ibbis.bio/our-work/common-mechanism/
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--- Log closed Tue Sep 03 00:00:41 2024