--- Log opened Thu Oct 03 00:00:09 2024
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03:10 < hprmbridge> kanzure> here is finalspark's chatroom https://x.com/finalsparkai/status/1841734968797450392
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13:43 < fenn> https://genius.com/Jamiroquai-virtual-insanity-lyrics he comes around at the end
13:52 <+gnusha_> https://secure.diyhpl.us/cgit/diyhpluswiki/commit/?id=29cc57f9 fenn: PGC-1α >> http://diyhpl.us/diyhpluswiki/genetic-modifications/
13:55 < fenn> because twitter is not an archval medium https://discord.com/invite/aayWQj9X8d
13:58 < hprmbridge> kanzure> thank you for your service
14:05 <+gnusha_> https://secure.diyhpl.us/cgit/diyhpluswiki/commit/?id=28d3d58d fenn: attempt to fix link >> http://diyhpl.us/diyhpluswiki/genetic-modifications/
14:05 <+gnusha_> https://secure.diyhpl.us/cgit/diyhpluswiki/commit/?id=4205234e fenn: attempt to fix link, try 2 >> http://diyhpl.us/diyhpluswiki/genetic-modifications/
14:06 < fenn> derp
14:06 <+gnusha_> https://secure.diyhpl.us/cgit/diyhpluswiki/commit/?id=4f544876 fenn: attempt to fix link, try 3 >> http://diyhpl.us/diyhpluswiki/genetic-modifications/
14:07 <+gnusha_> https://secure.diyhpl.us/cgit/diyhpluswiki/commit/?id=79303867 fenn: actually add the file >> http://diyhpl.us/diyhpluswiki/genetic-modifications.csv
14:09 < fenn> huh maybe the [[wikilink]] syntax would have worked if there were actually a file there
14:10 < jrayhawk> the web interface does have a preview button
14:11 < fenn> i thought that was broken
14:11 < jrayhawk> is it? oh
14:12 < jrayhawk> what's the problem with it?
14:12 < fenn> uh maybe i was just too chicken to type my root password into some web browser dialog
14:13 < fenn> but that's a me problem
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14:19 <+gnusha_> https://secure.diyhpl.us/cgit/diyhpluswiki/commit/?id=00870bcf fennwiki: the original [[wikilink]] syntax was correct, it just needed an existing file to point to >> http://diyhpl.us/diyhpluswiki/genetic-modifications/
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16:35 < fenn> this is just crazy and there doesn't seem to be much online about it. 40 grays in mice, that's 6x the lethal dose (10x in humans) https://www.dotmed.com/news/story/10571
16:35 < fenn> .t
16:35 < saxo> Scientists Make Mice Immune to Radiation
16:36 < fenn> "Radioprotection in Normal Tissue and Delayed Tumor Growth by Blockade of CD47 Signaling" https://www.science.org/doi/abs/10.1126/scitranslmed.3000139
16:40 < fenn> they only irradiated a limb
16:44 < fenn> also 1/10 the tumor volume vs control
16:45 < fenn> after 30 days
16:48 < nsh> it would be very surprising if the ill effects of radiation had a bottle neck in a particular signalling pathway
16:49 < nsh> probably significant smallprint here
16:49 < nsh> otoh it could well be that it's the body's response to the radiative damage that proves proximately lethal
16:50 < nsh> attenuating that probably makes things better but there's no escaping the effects of the ionisation itself that way
16:51 < nsh> oh okay it's in the context of radiotherapy for tumours
16:55 < nsh> there's such a tension between the complexity of what such things [e.g. CD47] do and these kinds of experiments which sound a lot like "what if just turned this down a bit"
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16:55 < nsh> reading https://en.wikipedia.org/wiki/CD47 there's half a dozen things some of which are very useful in fighting tumours
16:56 < nsh> some of which can result in [otherwise, prior to irradiation] healthy cells undergoing apoptosis
16:58 < nsh> .gpt4 how selectively can morpholino oligonucleotides be used to targeting CD47 signallying?   
16:58 < gptpaste> ​Morpholino oligonucleotides (MOs) are synthetic molecules used to modify gene expression, particularly in research settings. They are designed to bind to RNA transcripts and can block mRNA translation or modify splicing, thereby inhibiting the production of specific proteins. This makes them valuable tools for studying gene function and regulation.CD47 is a protein that is ex - https://gist.github.com/Epivalent/92722fb673c03fecff28b030ce03242b/raw
17:01 < nsh> .gpt4 specifically i was wondering about selectivity at a locus or wrt to cell types or kinds of organic tissue, rather than specificity wrt to the codons of or peripheral to the genes whose expression is being modified
17:01 < nsh> .gpt4 -with https://gist.github.com/Epivalent/92722fb673c03fecff28b030ce03242b/raw specifically i was wondering about selectivity at a locus or wrt to cell types or kinds of organic tissue, rather than specificity wrt to the codons of or peripheral to the genes whose expression is being modified
17:02 < gptpaste> ​Your question touches on an important aspect of gene expression and genetic engineering: the selectivity of targeting specific loci, cell types, or tissues for gene modification without affecting other regions or systems. This is crucial in both research and therapeutic contexts to ensure precise interventions with minimal off-target effects.### 1. **Selectivity at a Locus** - https://gist.github.com/Epivalent/aaca4ddb1dcfb1a6e671d7b2f3f710ac/raw
17:02 < gptpaste> ​Your question touches on an important aspect of gene expression and genetic engineering: the selectivity of targeting specific loci, cell types, or tissues for gene modification without affecting other regions or systems. This is crucial in both basic research and therapeutic applications to ensure precise control over genetic interventions and to minimize off-target effects. - https://gist.github.com/Epivalent/bbc457b7e060fd8aa5c87140119d3d5d/raw
17:03 < nsh> hmph
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--- Log closed Fri Oct 04 00:00:10 2024