--- Log opened Wed Nov 06 00:00:42 2024
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06:27 < kanzure> exa.ai/search continues to be awesome
06:36 < hprmbridge> alonzoc> wow. clean interface simple. actually gets you results
07:53 <+gnusha_> https://secure.diyhpl.us/cgit/diyhpluswiki/commit/?id=62461598 Bryan Bishop: add an old wtogami transcript about the mailing list >> http://diyhpl.us/diyhpluswiki/transcripts/bitcoin-core-dev-tech/2018-10-08-mailing-list/
07:53 <+gnusha_> https://secure.diyhpl.us/cgit/diyhpluswiki/commit/?id=0237a6b9 Bryan Bishop: Merge remote-tracking branch 'origin/master' into master >> http://diyhpl.us/diyhpluswiki/
07:53 <+gnusha_> https://secure.diyhpl.us/cgit/diyhpluswiki/commit/?id=dee8e5ad Bryan Bishop: genetic upregulation of fertility (increase number of oocytes) >> http://diyhpl.us/diyhpluswiki/genetic-modifications/
07:55 < kanzure> postnatal oogenesis https://www.sciencedirect.com/science/article/abs/pii/S0188440924001231
07:55 < kanzure> "Oocyte formation by mitotically-active germ cells purified from ovaries of reproductive age women" https://pmc.ncbi.nlm.nih.gov/articles/PMC3296965/
07:59 < kanzure> essentially obliterates the central dogma of human reproductive biology (born with all eggs)
08:00 < kanzure> but, absent that, increasing the number of oocytes through the germline seems like a very reasonable intervention, not sure why humans have the numbers that we do
08:00 < kanzure> could be 100x or something and not be geometrically at issue
08:03 < kanzure> i was looking at that because i was considering the insane idea to try to mitotically expand mature oocytes by intervening in the cell lifecycle regulators, and then manipulate them back into MII arrest after in vitro proliferation.
08:06 < kanzure> "Generation of functional oocytes from male mice in vitro" https://www.nature.com/articles/s41586-023-05834-x 
08:06 < kanzure> "Artificially produced euploid XX PS cells differentiated into mature oocytes in culture with similar efficiency to native XX PS cells. Using this method, we differentiated induced pluripotent stem cells from the tail of a sexually mature male mouse into fully potent oocytes, which gave rise to offspring after fertilization."
08:06 < kanzure> also this one:
08:06 < kanzure> "Induction of somatic cell haploidy by premature cell division" https://www.science.org/doi/10.1126/sciadv.adk9001
08:07 < kanzure> ".. an alternative in vitro gametogenesis approach by directly reprogramming diploid somatic cells into haploid oocytes."
09:04 < kanzure> "In vitro expansion of human sperm through nuclear transfer" https://pmc.ncbi.nlm.nih.gov/articles/PMC7118075/
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11:58 < hprmbridge> kanzure>  https://cdn.discordapp.com/attachments/1064664282450628710/1303810865195257896/image.png?ex=672d1bfd&is=672bca7d&hm=bb38ad5c6cd78f18d63486c8770be38dc9c157462e01f0207de87d2cd1384d94&
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15:12 < kanzure> web rot is real https://github.com/bitcoin/bitcoin/pull/29782#issuecomment-2460974096
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15:25 < kanzure> https://github.com/bitcointranscripts/bitcointranscripts/pull/566
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15:30 <+gnusha_> https://secure.diyhpl.us/cgit/diyhpluswiki/commit/?id=5ec348d3 Bryan Bishop: fix broken urls for lists.linuxfoundation.org in transcripts/ >> http://diyhpl.us/diyhpluswiki/transcripts/wasabi-research-club/2020-06-15-coinswap/
15:34 <+gnusha_> https://secure.diyhpl.us/cgit/diyhpluswiki/commit/?id=c821fbf5 Bryan Bishop: add uncommitted halfin transcript addition >> http://diyhpl.us/diyhpluswiki/transcripts/crypto98-hal-finney-zero-knowledge-proof-of-preimage/
15:39 < kanzure> oh look a mirror https://mailing-list.bitcoindevs.xyz/bitcoindev/
15:45 < kanzure> 2752 hits https://github.com/search?q=repo%3Abitcoinops%2Fbitcoinops.github.io%20lists.linuxfoundation.org&type=code
15:49 < kanzure> oof, lightning-dev urls are broken on diyhpluswiki.git now :\
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17:48 < hprmbridge> kanzure> https://www.lesswrong.com/posts/moqzbFTBMenwfw62S/twiblings-four-parent-babies-and-other-interesting
17:52 < hprmbridge> kanzure>  https://cdn.discordapp.com/attachments/1064664282450628710/1303899906909536256/image0.jpg?ex=672d6eeb&is=672c1d6b&hm=e90a4c90abf92683f384f36fa9b9dfbaec510998de42ee58b120163c62e30550&
17:53 < hprmbridge> kanzure> "Generation of human haploid embryonic stem cells from parthenogenetic embryos obtained by microsurgical removal of male pronucleus" https://www.nature.com/articles/cr201659
17:56 < hprmbridge> kanzure> "You can make a haploid stem cell line by tricking an egg into thinking it’s an embryo; you let a sperm fertilize it, then you pull out the sperm’s pronucleus before it fuses with that of the egg. Success! The egg now thinks it’s an embryo and will start dividing. So now you have a bunch of haploid embryonic stem cells, each of which is fairly epigenetically similar to an egg. But there’s still
17:56 < hprmbridge> kanzure> one tricky step left; how do you derive an egg from these haploid embryonic stem cells such that it can be fertilized again?"
17:57 < hprmbridge> kanzure> his solution of using nuclear transfer into a donor oocyte doesn't really solve the problem of not having enough oocytes.
17:58 < hprmbridge> kanzure> in a previous study in non-human, they used electronic fusion of haploid embryos to produce diploid embryos,  https://www.e-jarb.org/journal/view.html?uid=2610&vmd=Full
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18:49 < fenn> it's egg cloning
18:49 < fenn> i mean, assuming you can then turn the stem cells back into eggs
18:51 < fenn> how about harvesting eggs from developing embryos? they have millions of them
18:52 < fenn> is that bad somehow? we throw away millions of embryos already
19:15  * L29Ah allows
19:15 < L29Ah> the worst i can imagine is that it might create questionable feedback loops
19:17 < fenn> are they epigenetically similar to adult-harvested eggs?
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20:24 < hprmbridge> kanzure> you can do interspecies blastocyst complementation to grow human eggs
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20:25 < hprmbridge> kanzure> afaik you're not allowed to pay for aborted fetus parts
20:26 < hprmbridge> kanzure> you don't need to turn those stem cells back into true eggs.
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23:27 < fenn> well haploid stem cells don't sound very useful on their own
23:28 < fenn> what do you do with them if not make eggs?
23:29 < fenn> "add a sperm nucleus to make a viable embryo" sounds a lot like an egg
--- Log closed Thu Nov 07 00:00:42 2024