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06:13 < hprmbridge> yashgaroth> I guess, but luckily biology isn't hard. I don't think mixael is doing much beyond presentations at hackercons, and 99.99% of illicit schedule 1 synthesis is happily proceeding as it has for decades in camper vans or clandestine labs. There's a guy synthesizing HMX on youtube.
06:13 < hprmbridge> yashgaroth>
06:13 < hprmbridge> yashgaroth> DNA synthesis has gotten more annoying, and perhaps the only noticeable regulation on DIY biotech has been that oligo synthesizers are no longer as available on eBay. For now, setting up an LLC in a place that doesn't look like a house on google maps is enough to do pretty much whatever you want.
06:13 < hprmbridge> yashgaroth>
06:13 < hprmbridge> yashgaroth> If you want a fun enzymatic approach, feel free to take this up https://engineeringbiologycenter.org/wp-content/uploads/2017/10/Berry-IA-Pilot-Project.pdf https://engineeringbiologycenter.org/wp-content/uploads/2017/10/Berry-RMA-Pilot-Project-1.pdf . You need to synthesize the gene chip, but after that it's purely enzymatic
07:34 < hprmbridge> joedna_44104> Sorry, I meant schedule II, but it's still a similar stimulant that he made.  What made me very concerned about the ivory tower method of excluding anyone without a PhD/MD is that one of the biggest requests Mixael got at the end of his presentation for synthesis with the microlab was hormones.  They're probably better synthesized in eukaryotes but to get the genes to add to the biosynthetic
07:34 < hprmbridge> joedna_44104> pathways starting with cholesterol is prohibitively expensive, and now because of the three papers on drug manufacture with yeast--which isn't so fantastic, but who said the DEA cared?--I'll bet that getting anything over 10-24bpish PCR primers  is going to be prohibitively regulated--think DNA smurfs, lol--which leaves the masters and under crowd out in the cold.  I'm now personally invested in
07:34 < hprmbridge> joedna_44104> Josie and Mixael's success.  I just need to know more about proteomics which is why I'm hopefully getting back to grad school to take at least advanced biochem and molecular biology.  We did not do protein separation in biochem in undergrad and mostly focussed on combinatorial medical discovery and IG based concepts since the teacher was a Merck scientist who was an adjunct.
07:36 < hprmbridge> joedna_44104> Also, I took organic and biochem as a physics major so I didn't need to take the labs.  I spent that time in E&M and statistical physics lab.
07:38 < hprmbridge> joedna_44104> What I need is a nuclease that isn't 1/3 carbohydrates and is as ssDNA specific as Mung Bean.
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14:05 < hprmbridge> kanzure> https://youtu.be/evh0uzhW8v0?si=r2rZ7XS546Twl5bl
17:15 < hprmbridge> Eli> @jrayhawk I was looking more into the anti-oxidant idea. There seems to be multiple lines of evidence converging on it:
17:15 < hprmbridge> Eli> 1) Lung cancers have high frequencies of mutations that kick off endogenous anti-oxidant programs.
17:15 < hprmbridge> Eli> 2) Molecularly dissimilar exogenous anti-oxidants decrease ROS and DNA damage and rapidly increase tumor burden in mouse lung cancer models. The anti-oxidants modulates the ROS-P53 axis. In other words, anti-oxidants decrease smoke coming from the fire, thereby preventing the sprinklers from turning on which then leads to the building burning down.
17:15 < hprmbridge> Eli> 3) A number of pro-oxidative therapies have been developed to treat cancer. In fact, chemotherapy and radiotherapy primarily kill cancer cells via oxidative stress and DNA damage, which cancer overcomes by ... up-regulating endogenous anti-oxidants.
17:15 < hprmbridge> Eli>
17:15 < hprmbridge> Eli> I think I'm going to email the researcher who did the original mice lung cancer studies. Seems like something that could get funded. We'll see how it goes. Profs aren't always easy to get ahold of due to their schedules.
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17:38 < hprmbridge> Eli> Also, kind of a crazy story. The former CDC director said he's 100% certain that Covid was bioengineered. Also says that there is a concern that there's not really a way to precisely control the dose of proteins that the mrna vax produces (if I'm understanding him correctly). https://x.com/danaparish/status/1857115896046559619
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19:37 < hprmbridge> geraldmahony> The real question is, what experiment can you do to tell the difference
19:45 < hprmbridge> geraldmahony> Another question for you guys. On simulacra
19:46 < hprmbridge> geraldmahony> How do you determine if a person online is real or not
19:51 < jrayhawk> It would be best to assume any prior head of the CDC is a snake. Redfield hitching his wagon to Kennedy is not meaningful evidence of personal reform.
19:57 < jrayhawk> The CDC has many decades of history choosing prestige and money over science and public health. Fixing that culture involves retiring the snakes.
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20:10 < jrayhawk> Eli: you're sounding much more reasonable on the topic of cancer, so good job on your research. I will say statements like "Lung cancers have high frequencies of mutations that kick off endogenous anti-oxidant programs" is a statement that should immediately make you doubt the causal arrows that you've been provided; the lungs are already massive consumers of glutathione and one of the first organs 
20:10 < jrayhawk> to manifest life-threatening problems in its absence (fibrosis, asthma, pneumonia).
20:12 < jrayhawk> Mutations should cause a regression to the mean. They don't.
20:15 < jrayhawk> Cancer is caused by the complete or partial death of the mitochondria needed to manage apoptosis and inhibit energy consumption and growth. This can happen from any lipid or protein oxidation.
20:16 < jrayhawk> Oxidative stress kills the regulator of oxidative stress, which then increases oxidative stress. Detectable genetic mutations are very unpredictable and very heterogenous because they're not the causal factor for cancer.
20:24 < jrayhawk> Another interesting angle: the immune system gets pissed off at any cells throwing off large amounts of ROS and other cellular breakdown products, and attacks those cells to finish them off via oxidants. There is a selection effect that occurs there in two ways; slow cancer escapes the attention of the immune system, and fast-repairing and oxidation-mitigating survives the onslaught of the immune 
20:24 < jrayhawk> system.
20:24 < jrayhawk> er, [...] oxidation-mitigating cancer survives [...]
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23:49 < hprmbridge> Eli> Are there any seminal papers I can read on this?
--- Log closed Mon Nov 18 00:00:53 2024