--- Log opened Fri Sep 26 00:00:51 2025
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05:03 < hprmbridge> kanzure> how did that church methylation copy enzyme work
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08:16 < kanzure> ah, "epinoma"
08:22 < kanzure> https://epinoma.com/
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08:27 < jrayhawk> that's the worst trademark since Ayds
08:28 < kanzure> here is one possible methylation-preserving amplification chemistry: "DNA 5-methylcytosine-specific whole-genome amplification" by combining "strand displacement amplification (with phi29 polymerase) with DNMT1 methyltransferase in a single reaction" to retain retain 5mC marks during amplification.
08:28 < kanzure> (they might not be doing that)
08:34 < kanzure> DNMT1 finds hemimethylated strands and methylates the new strand? can't be that easy. someone would have done that by now.
08:45 < kanzure> https://europepmc.org/article/MED/32077696
09:12 < jrayhawk> https://www.youtube.com/watch?v=FAatHfrZEX4&t=7m28s meanwhile, in single-molecule long-read land
09:14 < kanzure> "mRNA splice isoform diversity" ugh
09:44 < hprmbridge> Eli> im gonna need to drink some more tylenol to understand this
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14:08 < Guest84> is there a log of ways to hack the brain?
14:11 < kanzure> no such page on the wiki for that right now, it sounds like a reasonable idea
14:11 < kanzure> Guest84: what do you have in mind? there's many techniques.
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14:25 < Guest84> A causes B to become ( horny, sleepy, angry, etc.)
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14:53 <+gnusha> https://secure.diyhpl.us/cgit/diyhpluswiki/commit/?id=6c1173ec Bryan Bishop: more bone stone >> http://diyhpl.us/diyhpluswiki/genetic-modifications/
14:53 <+gnusha> https://secure.diyhpl.us/cgit/diyhpluswiki/commit/?id=1034fcfc Bryan Bishop: mooore >> http://diyhpl.us/diyhpluswiki/in_vitro_fertilization/
14:58 < L29Ah> https://onlinelibrary.wiley.com/doi/10.1155/2024/5563513 seems like lemna produces much more DHA at colder temperature
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15:23 < Guest84> hmm
15:23 < Guest84> how do I see the log? i can't tell if anyone responded to my messages
15:23 < TMA> Guest84: look into the topic. the logs are at http://gnusha.org/logs
15:29 < TMA> Guest84: 
15:30 < TMA> Guest84: I guess it is a worthwhile research topic, be sure to share your results.
15:32 < Guest84> Sure
15:33 < TMA> The short answer that I know of is 
15:34 < TMA> "it is too complex and poorly understood for a useable short answer to exist at the moment"
15:35 < L29Ah> Guest84: you may explore the literature for 5HT2a agonists' impact on neuroplasticity/(un)learning while you're at it
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18:35 < L29Ah> https://www.theguardian.com/society/2025/sep/26/us-refuses-to-support-un-health-declaration-on-noncommunicable-diseases mexico the healthy state
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18:52 <+gnusha> https://secure.diyhpl.us/cgit/diyhpluswiki/commit/?id=d9482c83 Bryan Bishop: add motivation, reward, euphoria, anti-depression, etc >> http://diyhpl.us/diyhpluswiki/genetic-modifications/
18:52 < kanzure> that commit also contains some very bad ideas for oxidative damage protection
20:04 < hprmbridge> kanzure> genetic control of social status, hierarchy, dominance etc https://pmc.ncbi.nlm.nih.gov/articles/PMC7413536/
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21:19 < darsie> Can we make uploaded human minds more energy effiicent than current brains?
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23:33 < hprmbridge> kanzure> well we got a burn low for the last 10^50 years
23:35 < hprmbridge> kanzure> after which we must migrate from electron to lepton based substrate.
23:38 < hprmbridge> kanzure> er, i have that wrong. the baryonic barrier is much sooner than 10^50 years.
23:50 < hprmbridge> kanzure> The Old Ones were, the Old Ones are, and the Old Ones shall be.
23:54 < hprmbridge> kanzure> https://heybryan.org/docs/The%20Masque%20of%20the%20Heat%20Death%20-%20David%20Krieger.html
--- Log closed Sat Sep 27 00:00:51 2025