--- Log opened Fri Oct 10 00:00:04 2025
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04:07 < hprmbridge> kanzure> "antibody-mitochondria conjugates, which enable targeted mitochondrial delivery to specific cell populations"
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06:21 < L29Ah> https://github.com/mafik/keyer
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13:08 < hprmbridge> kanzure> fenn, do you want a scanning electron microscope? https://x.com/johndmcmaster/status/1976692808284315715
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13:30 < kanzure> a simple idea for cutting down on sybil attacks in online communities by using social network trust graph plus accountable introductions https://arxiv.org/abs/2007.15536
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15:45 < hprmbridge> kanzure> "Synthetically glycosylated antigens for the antigen-specific suppression of established immune responses" https://www.nature.com/articles/s41551-023-01086-2
15:46 < hprmbridge> kanzure> "Here we show that established antigen-specific responses in effector T cells and memory T cells can be suppressed by a polymer glycosylated with N-acetylgalactosamine (pGal) and conjugated to the antigen via a self-immolative linker that allows for the dissociation of the antigen on endocytosis and its presentation in the immunoregulatory environment."
15:47 < hprmbridge> kanzure> can u do that for insulin or tau
15:53 <+gnusha> https://secure.diyhpl.us/cgit/diyhpluswiki/commit/?id=a1e472fc Bryan Bishop: more immune >> http://diyhpl.us/diyhpluswiki/cell_therapy/
16:09 < hprmbridge> kanzure> "Firstborn IQ higher …likely via age-associated increases in maternal autoimmunity affecting fetal development"
16:18 < hprmbridge> .monokhrome> Maybe firstborns just tend to punch their younger siblings in the head a lot, that could explain it
16:18 < kanzure> well there are separation studies due to children raised apart
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18:08 < fenn> nowhere to put an electron microscope and no
18:10 < fenn> age-associated autoimmunity? not anti-fetal-parasite immunity?
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18:17 < fenn> keyer should have a CR2032 not a big honkin 18650
18:24 < L29Ah> CR2032 won't power Bluetooth for long, i vote for 18350
18:25 < L29Ah> or 14500 like Twiddler has
18:26 < L29Ah> speaking of Twiddler
18:26 < L29Ah> RangerMauve: what's your experience with it so far? are you touch-typing yet?
18:31 < fenn> those are all chunky. the goal is to make it form-fitting and not catch on stuff when you move around
18:32 < fenn> a pouch cell would also work, but charging circuitry would complicate the electronics
18:33 < L29Ah> charging circuitry is not a problem, you can grab some tiny off the shelf module
18:33 < L29Ah> and the biggest element would be the usb connector
19:05 < hprmbridge> kanzure> "Ballistic microscopy" https://www.biorxiv.org/content/10.1101/2025.10.07.681030v1 (2025)
19:05 < hprmbridge> kanzure> "Here we introduce a new approach, Ballistic Microscopy (BaM), to image cells with physical nanoparticles. We bombard living cells with millions of nanoparticles traveling at 1000 m/s. Each particle passes through cells, piercing and capturing attoliters of cytoplasm on a hydrogel substrate while preserving spatial information (SPLAT-MAP). This "physical image" of a live cell captures a molecular
19:05 < hprmbridge> kanzure> fingerprint of a cell on a hydrogel film that can be processed post-capture via multiple techniques such as TEM, Cryo-EM, mass spectrometry, confocal imaging, and DNA amplification."
19:07 < hprmbridge> kanzure> what's next, "SPLAT microscopy"? spatially projected lysis and transfer!
19:10 < hprmbridge> kanzure> it would be cool to combine this with expansion microscopy.
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19:12 < hprmbridge> kanzure> "Figure 3: Testing the BaM concept for collecting cytoplasm and membrane from live cells." do they survive?
19:13 < hprmbridge> kanzure> what if you could spin the gel down and recover and reconstitute the cytoplasmic contents into a new cell?
19:13 < hprmbridge> kanzure> via a gel extraction technique.
19:14 < hprmbridge> kanzure> maybe random FIB can also achieve some of this.
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19:16 < hprmbridge> kanzure> with SPLAT you could throw a cell against a hard surface and get radial data, or you could have it integrated into a gel surface, or you could do whole cell electrophoresis into a gel maybe.
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19:33 < hprmbridge> kanzure> if you spin the gel surface at a high speed while shooting the cell, you might be able to get better rotational resolution ?
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20:09 < hprmbridge> kanzure> maybe you could do live video/laser tracking of reflective particles, trajectory prediction, etc
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21:54 < hprmbridge> kanzure> "Primate superior colliculus is causally engaged in abstract higher-order cognition" https://pmc.ncbi.nlm.nih.gov/articles/PMC12068555/
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23:24 < fenn> huh. "self stirring mug" is a thing
23:24 < hprmbridge> kanzure>  https://cdn.discordapp.com/attachments/1064664282450628710/1426455498407809124/image0.jpg?ex=68eb49b5&is=68e9f835&hm=efde9770a5837cbccbb437eee80375268a699dbda606ffb159d3ddfbe06392e9&
23:27 < fenn> i don't understand why solder suckers weren't always like this https://www.amazon.com/Noah-Desoldering-Soldering-Portable-Precision/dp/B0F1Y3RVW9/
23:31 < fenn> i have finally managed to dissolve the creatine by boiling it in citric acid solution
23:31 < fenn> however it spontaneously crystallized after cooling
23:36 < hprmbridge> kanzure> inhibition of parabrachial Y1R neurons suppresses sustained pain https://www.nature.com/articles/s41586-025-09602-x
--- Log closed Sat Oct 11 00:00:05 2025