| --- Day changed Thu Feb 19 2009 | ||
| samrose | yep, got a slow response from http://heybryan.org/mediawiki/index.php/Skdb | 00:18 |
|---|---|---|
| kanzure | it's entirely possible that I'm currently splitting the account/connection. erm, it's hard to explain how this is working. | 00:22 |
| kanzure | (or it might not be) | 00:22 |
| kanzure | it might be getting confused as to which MAC address to submit packets to, since it's supposed to be one MAC address per account, and one of my internet accounts is inactive at the moment (apparently) | 00:22 |
| kanzure | anyway. | 00:22 |
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| kanzure | Hey davidnunez. | 08:09 |
| davidnunez | morning | 08:16 |
| kanzure | http://www.doviak.net/debian/debian_install_guide.pdf the illustrated guide to installing debian | 08:54 |
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| kanzure | samrose: hey | 11:30 |
| kanzure | samrose: Nathan and I are going to have a phone call sometime later today, to synchronize each other on things | 11:30 |
| kanzure | I was wondering if you met him when he was visiting Factor E Farm. | 11:30 |
| samrose | kanzure, I would love to join you. I will be in meetings all day today at MacArhtur Foundation Grantee Gathering, so I can't (unless I am msireading that you were offering for me to join :-) ) | 11:31 |
| kanzure | well, you misread, but you correctly predicted that Nathan and I would like the Factor E Farm guys, OSE, and such, and the Austin Fab Lab people to have a phone conference or something at some point | 11:33 |
| kanzure | http://www.dellsocialinnovationcompetition.com/apex/ideaView?id=087800000005GA5AAM The Farmery | 11:58 |
| kanzure | " The Farmery will be created with stacked shipping containers so that each container can be pre-manufactured and stacked to reduce production costs. Each container will have a 4 foot wide greenhouse attached to each long side. The crops will be grown hydroponically in vertical sacks holding the growing substrate that hang from rails inside the greenhouses that allow the crops to be rotated easily. Inside the container, specialty mushrooms will be grown. The bottom container will serve as the retail site for the food grown at the Farmery. The nutrients for the crops at the Farmery will come from composting local food wastes, so there is a constant cycle of turning the communities wastes into food." | 11:59 |
| kanzure | http://www.dellsocialinnovationcompetition.com/apex/ideaView?id=087800000005GA5AAM | 11:59 |
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| fenn | what are the conveyor belts for? just put a clear wall on the shipping containers and be done with it | 14:05 |
| fenn | 'if it ain't broke don't fix it" | 14:06 |
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| kanzure_bio | fenn: so it turns out the machine shop doesn't know how to convert the model to gcode (or doesn't have 'mastercam' or something). | 15:03 |
| kanzure_bio | score another point for "design for machinability" .. or not. | 15:03 |
| * kanzure_bio compiles openfoam in the background | 15:04 | |
| fenn | i think there are packages on opennovation.org | 15:20 |
| kanzure_bio | blah | 15:56 |
| kanzure_bio | the response on the diybio list to the dopamine synthesis pathways is awesome | 15:57 |
| kanzure_bio | I immediately got two IMs from Andrew Hessel, and one from Tito | 15:57 |
| kanzure_bio | as soon as it hit the mailing list. | 15:57 |
| kanzure | http://heybryan.org/~bbishop/docs/Step-and-scan%20maskless%20lithography%20for%20ultra%20large%20scale%20DNA%20chips.pdf | 16:51 |
| kanzure | A maskless photolithography test bed was constructed to examine the requirements for stepper-based synthesis of Ultra Large Scale DNA chips (ULS-DNA chips). The test bed is based on a microscope optical layout with a 5 × reduction imaging lens and micro/nano controlled staging at the image plane. Spatial light modulation is enabled by a Digital Micromirror Device (Texas Instruments DMD 0.7XGA) and the positioning system is composed of a piezoelectric nano-positioner (nPoint Inc., Madison, WI) mounted on a high precision linear-motor stage (Newport Corp., Irvine, CA). With this test bed we examined the requirements of overlay and alignment in a stepper-based DNA microarray synthesis system. We demonstrated multi-field chip synthesis with a spot size of 3.15 μm at the 5 × reduction. All tests were verified by standard hybridization, and fluorescence microscopy. In addition to our demonstration of step-and-scan lithography for DNA chip synthesis, we drafted and modeled an imaging optic for a production scale tool capable of synthesizing DNA chips containing up to 20 million pixels. | 16:51 |
| kanzure | http://www.sciencedirect.com/science?_ob=ArticleURL&_udi=B6V0W-4RMNYCM-5&_user=108429&_coverDate=06%2F30%2F2008&_rdoc=1&_fmt=full&_orig=search&_cdi=5657&_sort=d&_docanchor=&view=c&_acct=C000059713&_version=1&_urlVersion=0&_userid=108429&md5=5c61a528aecd8d80e044e5e0978ed957#fig1 | 16:51 |
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| xp_prg | where do I get adp1 and rdp? | 17:27 |
| kanzure | did you see the adp1 page on heybryan.org? | 17:33 |
| kanzure | http://heybryan.org/docs/Acinetobacter_instead_of_ecoli.html | 17:33 |
| xp_prg | on | 17:33 |
| xp_prg | sweet where do I get that man? | 17:34 |
| xp_prg | hello? | 17:39 |
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| xp_prg | kanzure are you ignoring me? | 17:45 |
| kanzure | no | 17:48 |
| kanzure | I was buying food. give me a break. | 17:49 |
| kanzure | you get it from a bacteria collection at some university | 17:49 |
| kanzure | look it up in the diybio mailing list archives | 17:49 |
| kanzure | http://groups.google.com/group/diybio | 17:49 |
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| kanzure | http://en.wikipedia.org/w/index.php?title=Natasha_Vita-More&diff=271954921&oldid=268703404 | 18:18 |
| kanzure | so.. | 18:18 |
| kanzure | hm, I have old #sl4 logs from 2002 now. | 18:27 |
| PeerInfinity | ooh, where did you get them from? | 18:35 |
| kanzure | Michael Anissimov | 18:39 |
| PeerInfinity | nice :) | 18:45 |
| kanzure | fenn: I'd eventually like to hear your opinion on dave | 19:07 |
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| kanzure | Hey Locke. | 19:10 |
| Locke | Hello | 19:10 |
| kanzure | Hey notTraveler. | 19:10 |
| notTraveler | hi, I'm just testing out the java IRC applet | 19:11 |
| notTraveler | http://cosmeng.org/chat/ | 19:11 |
| kanzure | Ah. | 19:11 |
| kanzure | http://heybryan.org/SSH2/ has a java SSH client that could be used to run irssi on a remote server | 19:12 |
| Locke | And I'm pretty much bound to the java IRC applet, not an IRC user naturally. | 19:12 |
| Locke | Another OCE member here. | 19:12 |
| kanzure | Hi there. | 19:12 |
| PeerInfinity | :) | 19:12 |
| kanzure | Did either of you see the "how to synthesize dopamine in a cell" post I made today? | 19:12 |
| PeerInfinity | ooh, you already figured out how to change your text color? | 19:13 |
| Locke | ...it's a single click, Peer. | 19:13 |
| notTraveler | ah, so it is :) | 19:13 |
| Locke | No, may I have a link? | 19:14 |
| PeerInfinity | hmm, the hplusroadmap archives only go up to July 2008: http://heybryan.org/pipermail/hplusroadmap/ | 19:22 |
| kanzure | sorry, my bad | 19:25 |
| kanzure | just had a chat with Natasha on the phone | 19:25 |
| kanzure | no, it was on the diybio list | 19:25 |
| kanzure | hrm, finding a link | 19:26 |
| kanzure | http://groups.google.com/group/diybio | 19:26 |
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| PeerInfinity | thanks for the link :) | 19:27 |
| * PeerInfinity looks at Locke's quit message and says in a cute bunny voice "I didn't do it..." | 19:28 | |
| kanzure | http://heybryan.org/~bbishop/docs/diybio.png | 19:37 |
| PeerInfinity | nice graph :) | 19:41 |
| bkero | kanzure: what's it a graph of? | 19:43 |
| kanzure | diybio :) | 19:43 |
| kanzure | I posted it a few moments ago | 19:43 |
| kanzure | On Thu, Feb 19, 2009 at 7:09 PM, Nick Taylor wrote: | 19:43 |
| kanzure | > This list is great btw. Every day a couple of things turn up that make me go | 19:43 |
| kanzure | > "holy crap!" | 19:43 |
| kanzure | so I replied with http://heybryan.org/~bbishop/docs/diybio.png | 19:43 |
| PeerInfinity | LOL! | 19:50 |
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| kanzure | hey gene. | 20:20 |
| gene | lolwut | 20:21 |
| kanzure | did you see the email I sent to diybio with the graph? | 20:21 |
| kanzure | On Thu, Feb 19, 2009 at 7:09 PM, Nick Taylor wrote: | 20:21 |
| kanzure | > This list is great btw. Every day a couple of things turn up that make me go | 20:21 |
| kanzure | > "holy crap!" | 20:21 |
| kanzure | so I replied with http://heybryan.org/~bbishop/docs/diybio.png | 20:21 |
| gene | heh | 20:21 |
| gene | I need to post the moore's law graph | 20:22 |
| kanzure | refresh that image | 20:26 |
| kanzure | I originally had a parabola, but an exponential seems better | 20:26 |
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| kanzure | phone call with Nathan. Need to call Marcin up eventually. | 21:03 |
| gene | huh | 21:04 |
| gene | some of my posts to diybio didn't show up | 21:04 |
| gene | google blox? | 21:04 |
| kanzure | which posts in particular? | 21:04 |
| kanzure | the group front page kind of hides them | 21:05 |
| kanzure | but they got through to me | 21:05 |
| kanzure | I got a few of them today, were there more? | 21:05 |
| gene | on the $25 | 21:05 |
| * kanzure looks over http://www.genome.ad.jp/dbget-bin/get_linkdb?-t+alldb+rn:R00345 | 21:05 | |
| gene | thermocycler | 21:05 |
| kanzure | yeah, it got through | 21:05 |
| gene | after josh | 21:05 |
| kanzure | " | 21:06 |
| kanzure | " | 21:06 |
| kanzure | I'm willing to bet we could make an illuminator using a tanning bed | 21:06 |
| kanzure | light or a UV disinfector. | 21:06 |
| kanzure | UV disinfector 185-365 nm, $139.99, but I think one might be able to | 21:06 |
| kanzure | find a cheaper one: | 21:06 |
| gene | wait a second | 21:06 |
| kanzure | " | 21:06 |
| gene | nope | 21:06 |
| gene | not it | 21:06 |
| kanzure | oh, after josh? | 21:06 |
| kanzure | hrm | 21:06 |
| gene | so reply to author means only they can see it | 21:06 |
| gene | ohhh | 21:06 |
| kanzure | hahah | 21:06 |
| gene | shit | 21:06 |
| kanzure | yeah :/ | 21:06 |
| gene | Now I have to repost | 21:07 |
| kanzure | blah, 2 hours remaining on the genes.tar.gz download from KEGG | 21:08 |
| gene | holy shit though easy bake thermocycler | 21:11 |
| kanzure | heh | 21:12 |
| gene | we should make one at the fablab | 21:13 |
| gene | BTW since the fab lab has a vacuum pump | 21:13 |
| kanzure | btw, sybr stains are like iodine drops added to the dna before you run your gel (IIRC) | 21:13 |
| gene | JUST IODINE! | 21:14 |
| gene | is it just iodine? | 21:14 |
| kanzure | no | 21:14 |
| gene | fuck | 21:14 |
| kanzure | it's something else | 21:14 |
| kanzure | sorry | 21:14 |
| kanzure | I wonder if the patent is out yet | 21:14 |
| kanzure | you could go look it up I guess | 21:14 |
| gene | if it was just iodine, we could do gels for dirt cheap | 21:15 |
| gene | none of that ethidium bromide crap | 21:15 |
| kanzure | or better yet, go look up the company that produces sybr gold, | 21:15 |
| kanzure | and then go look up when it was released (as a product) | 21:15 |
| gene | invitrogen | 21:15 |
| kanzure | then go look up who they hired a few years earlier | 21:15 |
| gene | google it | 21:15 |
| kanzure | and go read their theses | 21:15 |
| kanzure | and that will tell you what they were working on ;-) | 21:15 |
| kanzure | heh | 21:15 |
| gene | hahahahahahahahahahahahahahahahahahah | 21:15 |
| gene | since the fab lab has a vacuum pump | 21:16 |
| kanzure | btw, I have a call with carolina biological tomorrow | 21:16 |
| gene | there's something I'd like to try | 21:16 |
| kanzure | yeah? | 21:16 |
| gene | I want to make a room temperature quasi-superconductor | 21:16 |
| gene | carolina biological? | 21:16 |
| kanzure | it's like invitrogen except a smaller company I guess | 21:17 |
| gene | http://arxiv.org/abs/0805.0230 | 21:17 |
| gene | yeah I know them | 21:17 |
| gene | what are you calling them for | 21:17 |
| kanzure | I want them to see if they'd like to hook up their API to my XML biology protocols | 21:18 |
| gene | carolina is pretty diy biology friendly | 21:18 |
| kanzure | so that we can automatically buy parts for kits for diybio | 21:18 |
| kanzure | yeah | 21:18 |
| kanzure | so that's why I'm calling | 21:18 |
| kanzure | I'm talking with their ecommerce director | 21:18 |
| gene | you know there is a diybio meetup in San Francisco | 21:19 |
| kanzure | yes | 21:19 |
| gene | if they follow my suggestions | 21:20 |
| kanzure | did you see the diybio map? | 21:20 |
| gene | they might be able to get us some GFP and e.coli fairly cheaply | 21:20 |
| gene | you can clone the tubes of GFP gene right? | 21:20 |
| gene | same with lab grade e.coli too? | 21:21 |
| kanzure | "Microwave improved Escherichia coli transformation" | 21:23 |
| kanzure | "We found that giving calcium chloride competent cells a 1 min microwave pulse at the lowest power setting (180 W), instead of the classic 1–2 min 42°C heat-shock step, increases the transformation efficiency around threefold (3.3 ± 0.5). Moreover, when both treatments were given in a 2-min 42°C − 5 min on ice − 1 min microwave pluse sequence, an additional improvement of 1.6 was obtained, resulting in an overall increase in efficiency of approximately 5.3-fold compared to classical heat shock." | 21:24 |
| kanzure | http://www3.interscience.wiley.com/journal/119400964/abstract?CRETRY=1&SRETRY=0 | 21:24 |
| kanzure | "Significance and Impact of the Study: This method might be useful to those laboratories that cannot afford an electroporation apparatus." | 21:24 |
| gene | hahahahahaha | 21:26 |
| gene | nice | 21:26 |
| gene | post it in diybio | 21:26 |
| kanzure | okay. | 21:32 |
| kanzure | http://astrometry.net/ | 21:37 |
| kanzure | http://astrometry.net/gallery.html | 21:38 |
| kanzure | wonder if it's cross-referenced with respect to a star catalog | 21:38 |
| kanzure | one of the freaky awesome things to do would be to make it so that you could click the dots in the images, and find all other photos on flickr with those exact stars | 21:38 |
| kanzure | which would be one of those "holy shit these people are too spooky smart" | 21:38 |
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| kanzure | http://www.youtube.com/watch?v=v_p4t1Buv-I Amazing AeroGarden | 22:41 |
| kanzure | "The AeroGarden is the world's first indoor gardening appliance. Grow fresh herbs, produce, salad greens, flowers and more indoors, with no dirt, weeds or mess." | 22:41 |
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