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gene | hey is that pic from your harddrive? | 00:14 |
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gene | organize your stuff in a 2d not 1d manner | 00:19 |
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kanzure | " | 08:54 |
kanzure | I was actually thinking about this on the train this morning, and I was wondering if you could somehow use a human hair to make a really thin DIY microfluidics channel- like stretch it over the glass and then run the sharpie over it? But I guess the ink would probably run all around it. Maybe you could figure out some other way...paint a thin film of something over it and then pull it out?" | 08:54 |
kanzure | 80 µm — average width of human hair (ranges from 18 to 180 µm) | 08:54 |
kanzure | " | 08:55 |
kanzure | 10 µm — transistor width of the Intel 4004, the world's first commercial microprocessor" | 08:55 |
kanzure | gah, 10 micrometers != 18 micrometers. | 08:56 |
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kanzure | I'll go test hair+sharpie microfluidics on the microscope in about an hour | 09:11 |
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kanzure | hah hah hah | 12:48 |
kanzure | human hair doesn't work, even if you stretch it out with alligator clamps and hang it over the glass slide on which you are drawing | 12:48 |
kanzure | however, fingernails and paper clips do. | 12:48 |
fenn | fingernails and paperclips eh | 12:49 |
kanzure | yes. clear, smooth channels. | 12:49 |
kanzure | especially on the edge of a paper clip. It's about the same size as the hairs were. | 12:49 |
kanzure | the problem with hair was vibration, so I'd get not only one or two channels, but also something that looks like the "veins" from a leaf | 12:50 |
kanzure | and of course, the sharpie ink was actually being attracted to the individual hair fibers, soo. | 12:50 |
kanzure | there's no way that you're going to get micrometer accuracy on *both* a top and bottom slide though | 12:51 |
kanzure | unless you have a microscope and maybe a grid? | 12:51 |
fenn | or a really sharp pencil | 12:51 |
kanzure | yes. | 12:52 |
kanzure | erm, mechanical pencil much? | 12:56 |
fenn | what? | 12:56 |
kanzure | mechanical pencils tend to be better at fine patterns than sharp pencils | 12:57 |
fenn | they draw at about the width of the lead | 12:57 |
fenn | 0.5mm is nowhere near a 0.001mm | 12:57 |
kanzure | hm, I need to go back and double check the paperclip | 12:59 |
kanzure | the paperclip has a greater diameter than a typical mechanical pencil, but only a tiny portion of the edge was being used. | 13:01 |
fenn | any ideas on the micro CNC actuators? | 13:02 |
kanzure | http://heybryan.org/books/papers/microfluidics/macgyver_multitool.jpg | 13:02 |
kanzure | no. I once looked into pieco piezo actuators and they were "call to see prices" expensive. | 13:02 |
kanzure | but that was pico of course | 13:02 |
kanzure | *pico | 13:02 |
fenn | most non-consumer stuff will have RFQ | 13:03 |
kanzure | you know, I was adjusting the microscope by hand today with the knobs for xyz control | 13:10 |
kanzure | why couldn't that work? | 13:10 |
kanzure | besides the microscope control mechanism being ridiculously expensive (probably) | 13:11 |
fenn | more or less | 13:11 |
fenn | though microscopes aren't that expensive really | 13:11 |
fenn | and you get a microscope in the deal | 13:11 |
wrldpc | free microscopes? o.O | 13:19 |
kanzure | http://www.youtube.com/watch?v=LFoC-uxRqCg <-- gained 313,000 views since I last saw it. | 13:21 |
kanzure | (nano song) | 13:21 |
kanzure | http://nanosong.com/ | 13:25 |
fenn | that is pretty good | 13:30 |
fenn | production value! | 13:30 |
fenn | and grey goo! | 13:30 |
kanzure | yay it works on tape. | 13:38 |
kanzure | which is easier to acquire than glass. | 13:38 |
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xp_prg | kanzure what works on tape? | 15:36 |
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* fenn drools @ http://www.foothill.net/~sayre/22-in. binocular.htm | 16:40 | |
fenn | http://www.foothill.net/~sayre/22-in.%20binocular.htm for all you clickers | 16:40 |
fenn | i'm so making a handheld version | 16:41 |
fenn | or perhaps a head mount version | 16:43 |
fenn | or shoulder-mount like the predator | 16:43 |
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kanzure | heh, sata just became like my best friend :) | 17:34 |
kanzure | he asked me to make a mysql database | 17:34 |
kanzure | and then understood that there needs to be a data architecture/framework/definition | 17:34 |
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kanzure | http://www.youtube.com/watch?v=cL9Wu2kWwSY "Did you know?" presentation of various Big Numbers. got it in an email from my grandmother. | 17:56 |
kanzure | LOL, I can see where the scraping method would be preferable. Could you maybe take a comb-like device with little sharp metal bits embedded in the sides of the comb teeth, and glass slides stacked in between so that when you move the comb spine and the glass plates effectively move between the teeth you duplicate the same pattern on all?" | 17:57 |
kanzure | " | 17:57 |
kanzure | do'h. that's actually a smart idea, except that you would only be able to scrape linearly. | 17:58 |
kanzure | or, I mean, it wouldn't be able to do turns. | 17:59 |
kanzure | hm. if you had a stick with 'feet' at the end, and then moved that around between the glass slide sandwhich, that might work | 17:59 |
fenn | why no turns? | 18:03 |
fenn | bigger problem would be getting all the tips to press evenly | 18:04 |
kanzure | no turns if the entire device is the same width. but if only the tip has the width and only the tip is scraping the sharpie markings off, that's fine | 18:09 |
fenn | fail to parse | 18:11 |
kanzure | suppose you have a little stick that you shove between the two slides | 18:11 |
kanzure | at the end of this stick you have a thick piece that scrapes the sharpie off | 18:11 |
kanzure | if that was the case, that's ok and turns are possible | 18:12 |
fenn | why between two slides? | 18:12 |
kanzure | so that it scrapes the same pattern on both slides | 18:12 |
fenn | that's silly | 18:12 |
kanzure | well, do you have a better idea? | 18:12 |
fenn | why do you need the same pattern on both slides? | 18:12 |
kanzure | because apparently it doesn't work with sharpie only on one slide | 18:12 |
fenn | i dont see how that can work at all | 18:13 |
kanzure | ? | 18:13 |
fenn | have you actually done this? | 18:13 |
kanzure | yes | 18:13 |
kanzure | but I have not done "a glass sandwhich with only one side patterned" | 18:13 |
fenn | and it made the exact same pattern on two slides (one mirror reversed) | 18:13 |
kanzure | I have done "only one side patterned" and *not* in a glass sandwhich (it did not work) | 18:13 |
kanzure | no, I have not done that yet | 18:13 |
fenn | ok, well.. good luck i guess | 18:13 |
kanzure | I have used sharp pointy objects to draw in a dark splotch of sharpie though. | 18:14 |
kanzure | I need to see if hair works as a way to remove sharpie. | 18:14 |
fenn | why bother | 18:14 |
kanzure | hm? | 18:14 |
kanzure | because hair is thinner? | 18:14 |
fenn | chopped tungsten is pointier | 18:15 |
kanzure | I remember reading about that as a way to make tips for STMs | 18:15 |
kanzure | do I have tungsten laying around and I just don't know about it? | 18:15 |
fenn | you can get the same result with a paper clip | 18:15 |
kanzure | I broke a metal paper clip by bending/heating | 18:15 |
kanzure | but the sharp edge wasn't particularly sharp- I think there's a special way I have to break it | 18:15 |
fenn | you dont want to break it, cut it with wire cutters | 18:15 |
kanzure | wah, I don't have wire cutters laying around | 18:16 |
fenn | fingernail clippers? | 18:16 |
kanzure | no, but I'll get some | 18:16 |
fenn | it doesn't matter anyway | 18:16 |
kanzure | why? | 18:16 |
kanzure | (why does it not matter?) | 18:17 |
fenn | you need to get it to work with just one slide etched | 18:17 |
fenn | because there's no way in hell you're going to exactly duplicate the pattern on two slides with primitive methods | 18:17 |
kanzure | so, consider this: | 18:17 |
kanzure | when I did it the first time, I had two parallel lines as the design, so four lines in total | 18:18 |
kanzure | on one of them, the line was a fucked line | 18:18 |
kanzure | and went off in some weird direction | 18:18 |
kanzure | however, the bubble would follow the other line | 18:18 |
kanzure | no matter which way you flipped the device | 18:18 |
kanzure | in other words, it would not go off into the crazy direction | 18:18 |
fenn | ok i'm not surprised | 18:18 |
kanzure | so I should seriously try a sandwhich with only one side patterned | 18:18 |
kanzure | in fact, I'll go eat and then head off to the lab to check the paperclip-etched patterns in the microscope as well. | 18:19 |
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transilluminator | todo: (1) glass sandwhich with only one side patterned, the other side blank. (2) try it with tape-only (the non-sticky side- the other side can be taped to a sticky side of another piece) | 18:50 |
transilluminator | (3) sandwhich slides + simultaneous scraping (if #1 fails) | 18:51 |
transilluminator | also, I may be able to acquire photographs this time. | 18:52 |
* fenn squints | 18:52 | |
fenn | the new gel box has IRC capabilities? :) | 18:53 |
transilluminator | polIII hacked its way through the gel to make an IRC-compatible microfluidic circuit; please excuse the response delays. | 18:55 |
fenn | ubiquitin relay chat | 18:57 |
transilluminator | implementing IRC in one of these devices- whether as a genetic regulatory network or a microfluidic device (ASIC-like)-- would be sufficiently geeky to warrant a place on my todo list. | 18:58 |
fenn | and it would certainly make slashdot | 18:58 |
transilluminator | if you could ping proteins to speak with them, what would they say? what could be said with electrons? | 18:58 |
fenn | hurm. a protein isnt really complicated enough to do much, it's more of an analog component than digital, even though it's made in a digital process | 19:00 |
fenn | but theoretically you could do just about anything | 19:00 |
transilluminator | well, some proteins would act as an analog relay or signal processor, yes, that's true, | 19:00 |
fenn | report back the levels of various antigens | 19:00 |
transilluminator | but I was being more metaphorical than anything else | 19:00 |
transilluminator | more expecting a Douglas Adams response | 19:01 |
fenn | oh, uh. i guess they would probably mope for a while and then talk about the inevitable heat death of the universe | 19:01 |
transilluminator | har har, you made a funny about thermodynamics | 19:02 |
fenn | indeed | 19:02 |
transilluminator | enzyme kinematics too I guess | 19:02 |
fenn | now you get to go hit on some girls and ask them "would you like to come see my etchings?" | 19:03 |
transilluminator | hm, drawing sharpie on scotch tape when it's on glass doesn't work? | 19:05 |
transilluminator | but when the tape is on paper, it does work? except you peel off the tape and the sharpie pattern is not on the paper | 19:05 |
transilluminator | (I wrapped my slide in tape since I'd like to see if I can stop going through so many slides) | 19:05 |
transilluminator | oh, it's because it's going dry | 19:06 |
fenn | " Anybody | 19:11 |
fenn | with an inkjet printer and access to a lamination machine should be | 19:11 |
fenn | thinking about trying the double lamination ("polyester") layer | 19:11 |
fenn | microfluidics method instead of sharpies," | 19:11 |
fenn | man you really misunderstood that article didnt you | 19:11 |
transilluminator | hm? | 19:11 |
transilluminator | I also didn't readi t | 19:11 |
transilluminator | *read it | 19:11 |
fenn | sigh | 19:11 |
transilluminator | but I thought you said it was just a lamination approach? | 19:11 |
* transilluminator checks | 19:11 | |
transilluminator | (also, single-sided doesn't work) | 19:11 |
transilluminator | although I'm doing it on tape, which might be the problem | 19:11 |
fenn | process: print on transparency film with a laser printer, iron them together (well, you probably want a laminator for that) | 19:12 |
fenn | but it wont work with an inkjet | 19:12 |
transilluminator | oh, it has to be a laser printer? | 19:12 |
fenn | yes | 19:12 |
transilluminator | I must have been confusing it with http://heybryan.org/books/papers/microfluidics/Fabrication%20of%20microsensors%20using%20unmodified%20office%20inkjet%20printers.pdf | 19:12 |
transilluminator | or http://heybryan.org/books/papers/microfluidics/Refreshable%20microfluidic%20channels%20constructed%20using%20an%20inkjet%20printer.pdf | 19:12 |
transilluminator | wah. single-sided with tape doesn't work. | 19:13 |
transilluminator | the water just attached itself to the other slide. | 19:13 |
fenn | well i guess i'm off to the fabratory | 19:16 |
transilluminator | have fun | 19:17 |
transilluminator | did you correct me on diybio? | 19:17 |
transilluminator | ah, there we go | 19:18 |
transilluminator | thank you | 19:18 |
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fenn | hm so much for that idea | 19:47 |
transilluminator | no key? | 19:47 |
fenn | no :( | 19:48 |
fenn | i should talk to him about that, cause i'm pickling in my own uselessness | 19:48 |
transilluminator | mac asks "Is transparency "paper" hydrophilic? Because I know there are certain types that you can run directly through a laser printer. That would avoid the hot-iron mediated toner transfer step." | 19:50 |
fenn | there is no toner transfer step, you just run it through the printer | 19:50 |
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fenn | there's special "laser printer" version but i dont see much difference | 19:51 |
transilluminator | sharpie on one glass slide and nothing on the other slide does not work. | 19:51 |
transilluminator | also, sharpie on one side of a slide covered in cuiper tape doesn't work either | 19:51 |
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fenn | sigh.. diybio is too trivial for me | 19:51 |
transilluminator | (same with sharpie-side with scotch tape; same with both sides scotch tape, only one side patterned) | 19:51 |
transilluminator | trivial? | 19:52 |
fenn | its like a chatroom almost | 19:52 |
transilluminator | well. | 19:52 |
transilluminator | oh, the list | 19:52 |
transilluminator | I thought you were being upset about droplet herding :) | 19:52 |
fenn | the projects are lame too, but that's a different issue | 19:52 |
fenn | i wish the world would agree on top vs bottom posting | 19:55 |
transilluminator | srsly. | 19:55 |
fenn | i think guido had it right | 19:56 |
fenn | only one way to do it | 19:56 |
fenn | is best | 19:56 |
transilluminator | yes, most mailing lists have a policy for top versus bottom posting | 19:56 |
transilluminator | one time on piclist they decided on side posting, | 19:56 |
transilluminator | and then got into an argument on left/right posting | 19:56 |
transilluminator | bah. | 19:57 |
fenn | uh, side posting.. that's new | 19:57 |
transilluminator | one asshole did diagonal posting, but that's just ridicoulous. | 19:58 |
transilluminator | *ridiculous | 19:58 |
fenn | i like bottom posting because it's more or less like interleaved but you're replying to the whole message.. but i usually do whatever the rest of the thread is like. what chafes me is when someone switches halfway through the thread | 19:58 |
transilluminator | I have been finding that I am losing many minutes of my day now clicking on little "Show the quoted text" links in my mail clients for top-posters | 19:58 |
transilluminator | I have no freaking clue what they are responding to- literally- | 19:58 |
transilluminator | now, if I was in kmail, or another actual sane mail client, | 19:59 |
transilluminator | I'd have proper hierarchical threading | 19:59 |
transilluminator | but I guess nobody has written that particular greasemonkey userscript yet (wtf?) | 19:59 |
transilluminator | why am I using the gmail web interface | 19:59 |
fenn | what does greasemonkey have to do with it? | 19:59 |
fenn | gmail does IMAP so you have no excuse | 19:59 |
transilluminator | greasemonkey could be used to hack the thread display in a browser | 19:59 |
transilluminator | well, I want my archives in the same place | 20:00 |
transilluminator | gmail only allows a one-time download of mail | 20:00 |
fenn | one-time? | 20:00 |
transilluminator | and right now the laptop is "broken" | 20:00 |
transilluminator | yes | 20:00 |
fenn | oh, pop3 not IMAP? | 20:00 |
transilluminator | right | 20:00 |
fenn | http://mail.google.com/support/bin/answer.py?hl=en&answer=77695 | 20:00 |
transilluminator | okay okay | 20:01 |
transilluminator | I'll do that soon | 20:01 |
fenn | http://mail.google.com/support/bin/answer.py?answer=78799 | 20:01 |
transilluminator | so uh, any other ideas for one-sided fluidics? | 20:01 |
transilluminator | I've exhausted the available types of tape in the lab | 20:02 |
fenn | what was wrong with the laser printer idea? (polyester lamination) | 20:02 |
transilluminator | I don't have quick access to a lamination machine? | 20:03 |
fenn | clothes iron? | 20:03 |
fenn | propane torch and bar of metal? | 20:04 |
* fenn wonders what the minimum acceptable technology infrastructure is | 20:04 | |
transilluminator | no clothes iron :/ | 20:04 |
transilluminator | fenn: I certainly don't meet minimum requirements, in terms of what I am always allowed to access at any hour of the day etc. | 20:05 |
transilluminator | *minimum acceptable definition | 20:05 |
fenn | you should work on that | 20:05 |
fenn | oo hair curler | 20:05 |
transilluminator | why do women have more useful tools? | 20:06 |
transilluminator | hrm. | 20:06 |
transilluminator | it seems it must be double-sided. | 20:11 |
transilluminator | erm, done on both sides I mean | 20:11 |
transilluminator | oh | 20:14 |
transilluminator | http://heybryan.org/books/papers/microfluidics/Performing%20chemical%20reactions%20in%20virtual%20capillary%20of%20surface%20tension-confined%20microfluidic%20devices%20-%20sharpies%20-%20nail%20polish%20-%20glass%20surfaces%20-%20hydrophobicity.pdf | 20:14 |
transilluminator | so I think they used the nail polish so they could sandwhich the two slides together and imprint the pattern on the other one. | 20:14 |
transilluminator | "could be arbitrarily changed using appropriate pen and printing device" | 20:16 |
transilluminator | hm, " Next, we show that a patterned glass slide and the polycarbonate part of a CD | 20:18 |
transilluminator | can, when sandwiched appropriately, work as a microfluidic device and the same | 20:18 |
transilluminator | chemical reactions can be performed as well. In other words, a hydrophilic line sur- | 20:18 |
transilluminator | rounded by hydrophobic barrier, air and opposite hydrophobic surface is enough to | 20:18 |
transilluminator | " | 20:18 |
transilluminator | ink patterned glass side, and the hydrophobic side of the polycarbonate CD | 20:18 |
transilluminator | (the smooth / non-grooved side) | 20:19 |
fenn | guh.. you actually found a paper combining nail polish, sharpies, glass slides, and microfluidics? | 20:20 |
transilluminator | I was looking around for papers about nail polish and microfluidics | 20:22 |
transilluminator | and this one had the sharpie. | 20:22 |
transilluminator | are there any tricks to figure out which side is which on a CD? | 20:23 |
transilluminator | google is not being helpful | 20:24 |
fenn | the shiny side is the one you want | 20:24 |
transilluminator | they are both shiny | 20:24 |
fenn | the one you dont write on | 20:24 |
transilluminator | I usually just write on the one that is facing up when I pick it up from the stack that I bought it in. | 20:25 |
transilluminator | however, this CD has been picked up and has not preserved its flip state | 20:25 |
fenn | i think you need to go back to AI boot camp | 20:25 |
fenn | introduction to physical reality for agent based systems 101 | 20:26 |
transilluminator | near the doughnut hole, on one side, there is writing which cannot be seen from the other side of the doughnut hole | 20:26 |
transilluminator | the writing is in a circular subgroove or something. | 20:26 |
transilluminator | which side is this writing on? | 20:26 |
fenn | *facepalm* | 20:27 |
transilluminator | or actually, the doughnut hole (partially made of clear plastic) has writing which of course can only legibly be read from one side; which side is it when I can read it out? | 20:28 |
fenn | shiny side: http://commons.wikimedia.org/wiki/File:Compact_disc.svg | 20:28 |
fenn | the side you write on: http://commons.wikimedia.org/wiki/File:Compact_disc.jpg | 20:28 |
transilluminator | this disc does not have writing on it like that. | 20:29 |
transilluminator | I mean manufacturing information (supposedly) around the doughnot hole. | 20:29 |
transilluminator | both sides are shiny and give off rainbow light patterns. | 20:30 |
fenn | is it a lightscribe disk? | 20:30 |
transilluminator | one in particular seems particularly subsceptible to oils and grease | 20:30 |
transilluminator | I've never heard of lightscribe | 20:30 |
fenn | double sided, basically | 20:30 |
transilluminator | I guess it could be, how would I know? | 20:31 |
fenn | anyway since you're going to destroy it, the plastic side won't flake off when you scratch it with a knife | 20:31 |
fenn | but the aluminum side will | 20:31 |
fenn | if neither does, i can only assume it's some kind of sandwich | 20:31 |
transilluminator | it's certainly scratchable. | 20:31 |
transilluminator | but flake? | 20:31 |
fenn | it'll be transparent after you scratch the aluminum off | 20:32 |
transilluminator | oh shit | 20:32 |
transilluminator | yes, one side does flake :) | 20:32 |
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transilluminator | yay it's working | 20:37 |
transilluminator | woot one sided patterns. | 20:37 |
fenn | so, blob sharpie -> scratch -> clip 2 cd's together? | 20:38 |
transilluminator | so if you scratch the aluminum off, it will be transparent from the other side? | 20:39 |
transilluminator | so you'll be able to see what's going on? | 20:39 |
fenn | not exactly | 20:40 |
fenn | it'll be all scratched up | 20:40 |
fenn | i spose you could dissolve it with a strong base | 20:40 |
fenn | there are lots of other sources for clear relatively flat plastic | 20:41 |
transilluminator | yes, it is scratched, but you can at least see the sharpie pattern | 20:43 |
transilluminator | which means a dye would be visible | 20:43 |
transilluminator | which is sufficient methinks | 20:43 |
transilluminator | huh, apparently nail polish remover (acetone) on a cloth is sufficient to clean glass. | 20:50 |
transilluminator | *glass with sharpie markings on it | 20:50 |
fenn | acetone will dissolve just about anything | 20:53 |
fenn | if it really is nail polish remover it's probably ethyl acetate | 20:53 |
fenn | with other crap in it that might leave a film | 20:53 |
transilluminator | I suspect a lot of scrubbing with "plain" water will do the trick | 20:55 |
fenn | rubbing alcohol works (sorta) | 20:56 |
fenn | acetone will dissolve your CD though | 20:56 |
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willPow3r | http://www.jneuroengrehab.com/content/pdf/1743-0003-6-7.pdf | 20:59 |
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transilluminator | uberwin. | 21:09 |
transilluminator | using "Sanford Permanent Vis-a-Vis Overhead Projector Pen DO NOT SHAKE". | 21:10 |
transilluminator | (blue) | 21:10 |
transilluminator | sharpie blotch -> lay down some hair -> smash two glass slides together -> gently pull out the hair -> 18 to 180 micrometer channel. clear. | 21:11 |
fenn | i think the hair stuff is a bad idea | 21:11 |
transilluminator | it worked here. | 21:11 |
transilluminator | grr, I need a camera | 21:11 |
* transilluminator steals transilluminator camera | 21:11 | |
transilluminator | hm, it's locked | 21:12 |
transilluminator | but anyway, at best you'd just lay down hair in weird patterns and pull the hair on through | 21:13 |
transilluminator | I'm not sure you're going to be able to make specific patterns with this method | 21:13 |
katsmeow-afk | hold thehair str8, then sandwich it to get a str8 line | 21:18 |
katsmeow-afk | hold two hairs at angle, make sandwich, pull both out, then put a dot on the chanenls at the angle where you do not want a channel | 21:19 |
katsmeow-afk | no guarantee | 21:21 |
katsmeow-afk | the hairs may engage each otehr,, try to set the hair cuticle so it is less likely to engage | 21:23 |
katsmeow-afk | cuticle(?) | 21:23 |
katsmeow-afk | the ridges, the overlapping,, you know what i am trying to say | 21:23 |
transilluminator | I get about the same width from drawing with the edge of a paperclip as I do pulling my hair through a glass+sharpie sandwhich. | 21:29 |
transilluminator | (confirmed with microscope) | 21:29 |
fenn | the channel thickness is probably determined by the sharpie ink layer thickness | 21:30 |
fenn | darker = wider channel | 21:31 |
transilluminator | darker = wider channel in the vertical direction, you mean | 21:32 |
transilluminator | but remember there is also the 'spacer' between the glass layer and the polycarbonate (CD) or other glass surface | 21:32 |
transilluminator | I'm not even sure if the water is being confined to the precise rectangular (or fuzzy-nearly rectangular shape) etched out by the sharpie | 21:32 |
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