2009-03-06.log

--- Day changed Fri Mar 06 2009
genehey is that pic from your harddrive?00:14
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geneorganize your stuff in a 2d not 1d manner00:19
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kanzure"08:54
kanzureI was actually thinking about this on the train this morning, and I was wondering if you could somehow use a human hair to make a really thin DIY microfluidics channel- like stretch it over the glass and then run the sharpie over it? But I guess the ink would probably run all around it. Maybe you could figure out some other way...paint a thin film of something over it and then pull it out?"08:54
kanzure80 µm — average width of human hair (ranges from 18 to 180 µm)08:54
kanzure"08:55
kanzure10 µm — transistor width of the Intel 4004, the world's first commercial microprocessor"08:55
kanzuregah, 10 micrometers != 18 micrometers.08:56
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kanzureI'll go test hair+sharpie microfluidics on the microscope in about an hour09:11
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kanzurehah hah hah12:48
kanzurehuman hair doesn't work, even if you stretch it out with alligator clamps and hang it over the glass slide on which you are drawing12:48
kanzurehowever, fingernails and paper clips do.12:48
fennfingernails and paperclips eh12:49
kanzureyes. clear, smooth channels.12:49
kanzureespecially on the edge of a paper clip. It's about the same size as the hairs were.12:49
kanzurethe problem with hair was vibration, so I'd get not only one or two channels, but also something that looks like the "veins" from a leaf12:50
kanzureand of course, the sharpie ink was actually being attracted to the individual hair fibers, soo.12:50
kanzurethere's no way that you're going to get micrometer accuracy on *both* a top and bottom slide though12:51
kanzureunless you have a microscope and maybe a grid?12:51
fennor a really sharp pencil12:51
kanzureyes.12:52
kanzureerm, mechanical pencil much?12:56
fennwhat?12:56
kanzuremechanical pencils tend to be better at fine patterns than sharp pencils12:57
fennthey draw at about the width of the lead12:57
fenn0.5mm is nowhere near a 0.001mm12:57
kanzurehm, I need to go back and double check the paperclip12:59
kanzurethe paperclip has a greater diameter than a typical mechanical pencil, but only a tiny portion of the edge was being used.13:01
fennany ideas on the micro CNC actuators?13:02
kanzurehttp://heybryan.org/books/papers/microfluidics/macgyver_multitool.jpg13:02
kanzureno. I once looked into pieco piezo actuators and they were "call to see prices" expensive.13:02
kanzurebut that was pico of course13:02
kanzure*pico13:02
fennmost non-consumer stuff will have RFQ13:03
kanzureyou know, I was adjusting the microscope by hand today with the knobs for xyz control13:10
kanzurewhy couldn't that work?13:10
kanzurebesides the microscope control mechanism being ridiculously expensive (probably)13:11
fennmore or less13:11
fennthough microscopes aren't that expensive really13:11
fennand you get a microscope in the deal13:11
wrldpcfree microscopes? o.O13:19
kanzurehttp://www.youtube.com/watch?v=LFoC-uxRqCg <-- gained 313,000 views since I last saw it.13:21
kanzure(nano song)13:21
kanzurehttp://nanosong.com/13:25
fennthat is pretty good13:30
fennproduction value!13:30
fennand grey goo!13:30
kanzureyay it works on tape.13:38
kanzurewhich is easier to acquire than glass.13:38
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xp_prgkanzure what works on tape?15:36
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* fenn drools @ http://www.foothill.net/~sayre/22-in. binocular.htm16:40
fennhttp://www.foothill.net/~sayre/22-in.%20binocular.htm for all you clickers16:40
fenni'm so making a handheld version16:41
fennor perhaps a head mount version16:43
fennor shoulder-mount like the predator16:43
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kanzureheh, sata just became like my best friend :)17:34
kanzurehe asked me to make a mysql database17:34
kanzureand then understood that there needs to be a data architecture/framework/definition17:34
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kanzurehttp://www.youtube.com/watch?v=cL9Wu2kWwSY "Did you know?" presentation of various Big Numbers. got it in an email from my grandmother.17:56
kanzureLOL, I can see where the scraping method would be preferable. Could you maybe take a comb-like device with little sharp metal bits embedded in the sides of the comb teeth, and glass slides stacked in between so that when you move the comb spine and the glass plates effectively move between the teeth you duplicate the same pattern on all?"17:57
kanzure"17:57
kanzuredo'h. that's actually a smart idea, except that you would only be able to scrape linearly.17:58
kanzureor, I mean, it wouldn't be able to do turns.17:59
kanzurehm. if you had a stick with 'feet' at the end, and then moved that around between the glass slide sandwhich, that might work17:59
fennwhy no turns?18:03
fennbigger problem would be getting all the tips to press evenly18:04
kanzureno turns if the entire device is the same width. but if only the tip has the width and only the tip is scraping the sharpie markings off, that's fine18:09
fennfail to parse18:11
kanzuresuppose you have a little stick that you shove between the two slides18:11
kanzureat the end of this stick you have a thick piece that scrapes the sharpie off18:11
kanzureif that was the case, that's ok and turns are possible18:12
fennwhy between two slides?18:12
kanzureso that it scrapes the same pattern on both slides18:12
fennthat's silly18:12
kanzurewell, do you have a better idea?18:12
fennwhy do you need the same pattern on both slides?18:12
kanzurebecause apparently it doesn't work with sharpie only on one slide18:12
fenni dont see how that can work at all18:13
kanzure?18:13
fennhave you actually done this?18:13
kanzureyes18:13
kanzurebut I have not done "a glass sandwhich with only one side patterned"18:13
fennand it made the exact same pattern on two slides (one mirror reversed)18:13
kanzureI have done "only one side patterned" and *not* in a glass sandwhich (it did not work)18:13
kanzureno, I have not done that yet18:13
fennok, well.. good luck i guess18:13
kanzureI have used sharp pointy objects to draw in a dark splotch of sharpie though.18:14
kanzureI need to see if hair works as a way to remove sharpie.18:14
fennwhy bother18:14
kanzurehm?18:14
kanzurebecause hair is thinner?18:14
fennchopped tungsten is pointier18:15
kanzureI remember reading about that as a way to make tips for STMs18:15
kanzuredo I have tungsten laying around and I just don't know about it?18:15
fennyou can get the same result with a paper clip18:15
kanzureI broke a metal paper clip by bending/heating18:15
kanzurebut the sharp edge wasn't particularly sharp- I think there's a special way I have to break it18:15
fennyou dont want to break it, cut it with wire cutters18:15
kanzurewah, I don't have wire cutters laying around18:16
fennfingernail clippers?18:16
kanzureno, but I'll get some18:16
fennit doesn't matter anyway18:16
kanzurewhy?18:16
kanzure(why does it not matter?)18:17
fennyou need to get it to work with just one slide etched18:17
fennbecause there's no way in hell you're going to exactly duplicate the pattern on two slides with primitive methods18:17
kanzureso, consider this:18:17
kanzurewhen I did it the first time, I had two parallel lines as the design, so four lines in total18:18
kanzureon one of them, the line was a fucked line18:18
kanzureand went off in some weird direction18:18
kanzurehowever, the bubble would follow the other line18:18
kanzureno matter which way you flipped the device18:18
kanzurein other words, it would not go off into the crazy direction18:18
fennok i'm not surprised18:18
kanzureso I should seriously try a sandwhich with only one side patterned18:18
kanzurein fact, I'll go eat and then head off to the lab to check the paperclip-etched patterns in the microscope as well.18:19
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transilluminatortodo: (1) glass sandwhich with only one side patterned, the other side blank. (2) try it with tape-only (the non-sticky side- the other side can be taped to a sticky side of another piece)18:50
transilluminator(3) sandwhich slides + simultaneous scraping (if #1 fails)18:51
transilluminatoralso, I may be able to acquire photographs this time.18:52
* fenn squints18:52
fennthe new gel box has IRC capabilities? :)18:53
transilluminatorpolIII hacked its way through the gel to make an IRC-compatible microfluidic circuit; please excuse the response delays.18:55
fennubiquitin relay chat18:57
transilluminatorimplementing IRC in one of these devices- whether as a genetic regulatory network or a microfluidic device (ASIC-like)--  would be sufficiently geeky to warrant a place on my todo list.18:58
fennand it would certainly make slashdot18:58
transilluminatorif you could ping proteins to speak with them, what would they say? what could be said with electrons?18:58
fennhurm. a protein isnt really complicated enough to do much, it's more of an analog component than digital, even though it's made in a digital process19:00
fennbut theoretically you could do just about anything19:00
transilluminatorwell, some proteins would act as an analog relay or signal processor, yes, that's true,19:00
fennreport back the levels of various antigens19:00
transilluminatorbut I was being more metaphorical than anything else19:00
transilluminatormore expecting a Douglas Adams response19:01
fennoh, uh. i guess they would probably mope for a while and then talk about the inevitable heat death of the universe19:01
transilluminatorhar har, you made a funny about thermodynamics19:02
fennindeed19:02
transilluminatorenzyme kinematics too I guess19:02
fennnow you get to go hit on some girls and ask them "would you like to come see my etchings?"19:03
transilluminatorhm, drawing sharpie on scotch tape when it's on glass doesn't work?19:05
transilluminatorbut when the tape is on paper, it does work? except you peel off the tape and the sharpie pattern is not on the paper19:05
transilluminator(I wrapped my slide in tape since I'd like to see if I can stop going through so many slides)19:05
transilluminatoroh, it's because it's going dry19:06
fenn" Anybody19:11
fennwith an inkjet printer and access to a lamination machine should be19:11
fennthinking about trying the double lamination ("polyester") layer19:11
fennmicrofluidics method instead of sharpies,"19:11
fennman you really misunderstood that article didnt you19:11
transilluminatorhm?19:11
transilluminatorI also didn't readi t19:11
transilluminator*read it19:11
fennsigh19:11
transilluminatorbut I thought you said it was just a lamination approach?19:11
* transilluminator checks19:11
transilluminator(also, single-sided doesn't work)19:11
transilluminatoralthough I'm doing it on tape, which might be the problem19:11
fennprocess: print on transparency film with a laser printer, iron them together (well, you probably want a laminator for that)19:12
fennbut it wont work with an inkjet19:12
transilluminatoroh, it has to be a laser printer?19:12
fennyes19:12
transilluminatorI must have been confusing it with http://heybryan.org/books/papers/microfluidics/Fabrication%20of%20microsensors%20using%20unmodified%20office%20inkjet%20printers.pdf19:12
transilluminatoror http://heybryan.org/books/papers/microfluidics/Refreshable%20microfluidic%20channels%20constructed%20using%20an%20inkjet%20printer.pdf19:12
transilluminatorwah. single-sided with tape doesn't work.19:13
transilluminatorthe water just attached itself to the other slide.19:13
fennwell i guess i'm off to the fabratory19:16
transilluminatorhave fun19:17
transilluminatordid you correct me on diybio?19:17
transilluminatorah, there we go19:18
transilluminatorthank you19:18
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fennhm so much for that idea19:47
transilluminatorno key?19:47
fennno :(19:48
fenni should talk to him about that, cause i'm pickling in my own uselessness19:48
transilluminatormac asks "Is transparency "paper" hydrophilic?  Because I know there are certain types that you can run directly through a laser printer.  That would avoid the hot-iron mediated toner transfer step."19:50
fennthere is no toner transfer step, you just run it through the printer19:50
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fennthere's special "laser printer" version but i dont see much difference19:51
transilluminatorsharpie on one glass slide and nothing on the other slide does not work.19:51
transilluminatoralso, sharpie on one side of a slide covered in cuiper tape doesn't work either19:51
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fennsigh.. diybio is too trivial for me19:51
transilluminator(same with sharpie-side with scotch tape; same with both sides scotch tape, only one side patterned)19:51
transilluminatortrivial?19:52
fennits like a chatroom almost19:52
transilluminatorwell.19:52
transilluminatoroh, the list19:52
transilluminatorI thought you were being upset about droplet herding :)19:52
fennthe projects are lame too, but that's a different issue19:52
fenni wish the world would agree on top vs bottom posting19:55
transilluminatorsrsly.19:55
fenni think guido had it right19:56
fennonly one way to do it19:56
fennis best19:56
transilluminatoryes, most mailing lists have a policy for top versus bottom posting19:56
transilluminatorone time on piclist they decided on side posting,19:56
transilluminatorand then got into an argument on left/right posting19:56
transilluminatorbah.19:57
fennuh, side posting.. that's new19:57
transilluminatorone asshole did diagonal posting, but that's just ridicoulous.19:58
transilluminator*ridiculous19:58
fenni like bottom posting because it's more or less like interleaved but you're replying to the whole message.. but i usually do whatever the rest of the thread is like. what chafes me is when someone switches halfway through the thread19:58
transilluminatorI have been finding that I am losing many minutes of my day now clicking on little "Show the quoted text" links in my mail clients for top-posters19:58
transilluminatorI have no freaking clue what they are responding to- literally-19:58
transilluminatornow, if I was in kmail, or another actual sane mail client,19:59
transilluminatorI'd have proper hierarchical threading19:59
transilluminatorbut I guess nobody has written that particular greasemonkey userscript yet (wtf?)19:59
transilluminatorwhy am I using the gmail web interface19:59
fennwhat does greasemonkey have to do with it?19:59
fenngmail does IMAP so you have no excuse19:59
transilluminatorgreasemonkey could be used to hack the thread display in a browser19:59
transilluminatorwell, I want my archives in the same place20:00
transilluminatorgmail only allows a one-time download of mail20:00
fennone-time?20:00
transilluminatorand right now the laptop is "broken"20:00
transilluminatoryes20:00
fennoh, pop3 not IMAP?20:00
transilluminatorright20:00
fennhttp://mail.google.com/support/bin/answer.py?hl=en&answer=7769520:00
transilluminatorokay okay20:01
transilluminatorI'll do that soon20:01
fennhttp://mail.google.com/support/bin/answer.py?answer=7879920:01
transilluminatorso uh, any other ideas for one-sided fluidics?20:01
transilluminatorI've exhausted the available types of tape in the lab20:02
fennwhat was wrong with the laser printer idea? (polyester lamination)20:02
transilluminatorI don't have quick access to a lamination machine?20:03
fennclothes iron?20:03
fennpropane torch and bar of metal?20:04
* fenn wonders what the minimum acceptable technology infrastructure is20:04
transilluminatorno clothes iron :/20:04
transilluminatorfenn: I certainly don't meet minimum requirements, in terms of what I am always allowed to access at any hour of the day etc.20:05
transilluminator*minimum acceptable definition20:05
fennyou should work on that20:05
fennoo hair curler20:05
transilluminatorwhy do women have more useful tools?20:06
transilluminatorhrm.20:06
transilluminatorit seems it must be double-sided.20:11
transilluminatorerm, done on both sides I mean20:11
transilluminatoroh20:14
transilluminatorhttp://heybryan.org/books/papers/microfluidics/Performing%20chemical%20reactions%20in%20virtual%20capillary%20of%20surface%20tension-confined%20microfluidic%20devices%20-%20sharpies%20-%20nail%20polish%20-%20glass%20surfaces%20-%20hydrophobicity.pdf20:14
transilluminatorso I think they used the nail polish so they could sandwhich the two slides together and imprint the pattern on the other one.20:14
transilluminator"could be arbitrarily changed using appropriate pen and printing device"20:16
transilluminatorhm, "  Next, we show that a patterned glass slide and the polycarbonate part of a CD20:18
transilluminatorcan, when sandwiched appropriately, work as a microfluidic device and the same20:18
transilluminatorchemical reactions can be performed as well. In other words, a hydrophilic line sur-20:18
transilluminatorrounded by hydrophobic barrier, air and opposite hydrophobic surface is enough to20:18
transilluminator"20:18
transilluminatorink patterned glass side, and the hydrophobic side of the polycarbonate CD20:18
transilluminator(the smooth / non-grooved side)20:19
fennguh.. you actually found a paper combining nail polish, sharpies, glass slides, and microfluidics?20:20
transilluminatorI was looking around for papers about nail polish and microfluidics20:22
transilluminatorand this one had the sharpie.20:22
transilluminatorare there any tricks to figure out which side is which on a CD?20:23
transilluminatorgoogle is not being helpful20:24
fennthe shiny side is the one you want20:24
transilluminatorthey are both shiny20:24
fennthe one you dont write on20:24
transilluminatorI usually just write on the one that is facing up when I pick it up from the stack that I bought it in.20:25
transilluminatorhowever, this CD has been picked up and has not preserved its flip state20:25
fenni think you need to go back to AI boot camp20:25
fennintroduction to physical reality for agent based systems 10120:26
transilluminatornear the doughnut hole, on one side, there is writing which cannot be seen from the other side of the doughnut hole20:26
transilluminatorthe writing is in a circular subgroove or something.20:26
transilluminatorwhich side is this writing on?20:26
fenn*facepalm*20:27
transilluminatoror actually, the doughnut hole (partially made of clear plastic)  has writing which of course can only legibly be read from one side; which side is it when I can read it out?20:28
fennshiny side: http://commons.wikimedia.org/wiki/File:Compact_disc.svg20:28
fennthe side you write on: http://commons.wikimedia.org/wiki/File:Compact_disc.jpg20:28
transilluminatorthis disc does not have writing on it like that.20:29
transilluminatorI mean manufacturing information (supposedly) around the doughnot hole.20:29
transilluminatorboth sides are shiny and give off rainbow light patterns.20:30
fennis it a lightscribe disk?20:30
transilluminatorone in particular seems particularly subsceptible to oils and grease20:30
transilluminatorI've never heard of lightscribe20:30
fenndouble sided, basically20:30
transilluminatorI guess it could be, how would I know?20:31
fennanyway since you're going to destroy it, the plastic side won't flake off when you scratch it with a knife20:31
fennbut the aluminum side will20:31
fennif neither does, i can only assume it's some kind of sandwich20:31
transilluminatorit's certainly scratchable.20:31
transilluminatorbut flake?20:31
fennit'll be transparent after you scratch the aluminum off20:32
transilluminatoroh shit20:32
transilluminatoryes, one side does flake :)20:32
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transilluminatoryay it's working20:37
transilluminatorwoot one sided patterns.20:37
fennso, blob sharpie -> scratch -> clip 2 cd's together?20:38
transilluminatorso if you scratch the aluminum off, it will be transparent from the other side?20:39
transilluminatorso you'll be able to see what's going on?20:39
fennnot exactly20:40
fennit'll be all scratched up20:40
fenni spose you could dissolve it with a strong base20:40
fennthere are lots of other sources for clear relatively flat plastic20:41
transilluminatoryes, it is scratched, but you can at least see the sharpie pattern20:43
transilluminatorwhich means a dye would be visible20:43
transilluminatorwhich is sufficient methinks20:43
transilluminatorhuh, apparently nail polish remover (acetone) on a cloth is sufficient to clean glass.20:50
transilluminator*glass with sharpie markings on it20:50
fennacetone will dissolve just about anything20:53
fennif it really is nail polish remover it's probably ethyl acetate20:53
fennwith other crap in it that might leave a film20:53
transilluminatorI suspect a lot of scrubbing with "plain" water will do the trick20:55
fennrubbing alcohol works (sorta)20:56
fennacetone will dissolve your CD though20:56
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willPow3rhttp://www.jneuroengrehab.com/content/pdf/1743-0003-6-7.pdf20:59
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transilluminatoruberwin.21:09
transilluminatorusing "Sanford Permanent Vis-a-Vis Overhead Projector Pen DO NOT SHAKE".21:10
transilluminator(blue)21:10
transilluminatorsharpie blotch -> lay down some hair -> smash two glass slides together -> gently pull out the hair -> 18 to 180 micrometer channel. clear.21:11
fenni think the hair stuff is a bad idea21:11
transilluminatorit worked here.21:11
transilluminatorgrr, I need a camera21:11
* transilluminator steals transilluminator camera21:11
transilluminatorhm, it's locked21:12
transilluminatorbut anyway, at best you'd just lay down hair in weird patterns and pull the hair on through21:13
transilluminatorI'm not sure you're going to be able to make specific patterns with this method21:13
katsmeow-afkhold thehair str8, then sandwich it to get a str8 line21:18
katsmeow-afkhold two hairs at angle, make sandwich, pull both out, then put a dot on the chanenls at the angle where you do not want a channel21:19
katsmeow-afkno guarantee21:21
katsmeow-afkthe hairs may engage each otehr,, try to set the hair cuticle so it is less likely to engage21:23
katsmeow-afkcuticle(?)21:23
katsmeow-afkthe ridges, the overlapping,, you know what i am trying to say21:23
transilluminatorI get about the same width from drawing with the edge of a paperclip as I do pulling my hair through a glass+sharpie sandwhich.21:29
transilluminator(confirmed with microscope)21:29
fennthe channel thickness is probably determined by the sharpie ink layer thickness21:30
fenndarker = wider channel21:31
transilluminatordarker = wider channel in the vertical direction, you mean21:32
transilluminatorbut remember there is also the 'spacer' between the glass layer and the polycarbonate (CD) or other glass surface21:32
transilluminatorI'm not even sure if the water is being confined to the precise rectangular (or fuzzy-nearly rectangular shape) etched out by the sharpie21:32
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