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gene | heh I got some spam from them | 01:31 |
---|---|---|
gene | IRL spam | 01:31 |
gene | mail from them a while back | 01:31 |
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kanzure | "Hydrophilic/hydrophobic patterned surfaces as templates for DNA arrays" | 01:45 |
gene | muahahahahahahahahh | 01:53 |
gene | http://s88.photobucket.com/albums/k200/elbuenohombre89/?action=view¤t=GendoIkari.jpg | 01:55 |
gene | that's pretty much how I am after hearing that | 01:55 |
gene | wait a minute, the psp has a 480 x 272 screen | 01:57 |
gene | that can't be right? | 01:57 |
kanzure | http://ukpmc.ac.uk/articlerender.cgi?artid=1545998 | 01:58 |
kanzure | Recursive construction of perfect DNA molecules from imperfect oligonucleotides | 01:58 |
gene | hmmmm.. | 01:59 |
gene | dna repair | 01:59 |
gene | dinosaur dna is damaged | 01:59 |
gene | dinosaurs might be profitable... | 01:59 |
kanzure | I'm trying to find a paper that gives an analysis of the sources of error and error rates in conventional oligo synthesis techniques. | 03:00 |
gene | damn I need to send you that stuff | 03:00 |
kanzure | http://en.wikipedia.org/wiki/Oligonucleotide_synthesis "s the number of errors increases with the length of the oligonucleotide sequence that is being synthesized. " | 03:00 |
kanzure | [citation needed] | 03:00 |
kanzure | seriously | 03:00 |
gene | dude why can't we bring back dinosaurs with DNA error correction? | 03:04 |
gene | I belive I found a solution to head mounted display mounting | 03:12 |
gene | would you like to know? | 03:13 |
gene | you put acouple of titanium mounting brackets into your skull | 03:14 |
gene | http://interactive.usc.edu/members/mark/archives/wide5.jpg | 03:15 |
gene | yikes, we might not want to use a LCD screen | 03:16 |
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gene | http://api.ning.com/files/KLxUkA8D81DH2fz4hfju4aa*QmoBff1yaOFTJuEuj9AWjhwozdtCjoip5sSQ6k-k1tWeN9P85HmIHRVbD2KvaR6B9vPuPDWU/cyberpunk_hacker_by_mercikos.jpg | 03:18 |
gene | might have to go all out in that case | 03:19 |
gene | http://www.jewellersequipment.co.uk/eyeglasses/LHMG.JPG | 03:25 |
gene | A SOLUTION HAS BEEN FOUND | 03:25 |
kanzure | http://scholar.google.com/scholar?as_q=&num=100&btnG=Search+Scholar&as_epq=&as_oq=&as_eq=&as_occt=any&as_sauthors=Khorana&as_publication=&as_ylo=1960&as_yhi=1970&as_allsubj=all&hl=en&lr= | 03:27 |
gene | kanzure, magnifier glasses | 03:34 |
gene | are the solution | 03:34 |
gene | we just put LCD's on them | 03:35 |
gene | LCDs with cameras for AR | 03:35 |
gene | steve mann gets 640X480 | 03:40 |
gene | DLP is starting to tempt me | 03:42 |
gene | 1400x1050 | 03:43 |
gene | is dlp | 03:43 |
gene | wait a second | 03:43 |
gene | that's it? | 03:43 |
gene | hmmm... | 03:43 |
gene | I think I just realized something | 03:43 |
kanzure | Syringe method for stepwise chemical synthesis of oligonucleotides http://www.pubmedcentral.nih.gov/picrender.fcgi?artid=320704&blobtype=pdf | 03:47 |
kanzure | Synthesis of a gene for human growth hormone and its expression in Escherichia coli | 03:47 |
gene | with a syringe? | 03:48 |
gene | how long does that take? | 03:48 |
kanzure | if you use zinc, 30 minutes per nucleotide | 03:51 |
kanzure | but if you don't, it usually takes much less apparently | 03:51 |
gene | HAHAHAHAHAHAHAHAHAHAHAHAHAHAHAHAHAHAHAHAHAHAHAHAHHAHAHAHAHAHAHAHHAHAHAA | 03:51 |
gene | no | 03:51 |
kanzure | gene: become a monk! synthesize your own genome by syringe! | 03:52 |
kanzure | "oh fuck, I made an error" | 03:52 |
gene | at 30 minutes per nucleotide it would take OVER 9000 hrs to synthesize the ebola genome | 03:53 |
gene | no joke | 03:53 |
gene | or 375 dayw | 03:53 |
gene | days | 03:53 |
gene | few | 03:53 |
gene | terrorists won't be able to do that | 03:53 |
gene | they have to be 4 levels above batshit insane | 03:55 |
gene | and have a lot of time | 03:57 |
kanzure | syringe method takes 13 min per nucleotide. haha. | 04:02 |
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kanzure | Coriolis force? | 09:35 |
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fenn | for your algae filter can't you use something like this with a really fine mesh? http://images.google.com/imgres?imgurl=http://www.home-food-processing.com/ProductImages/32164.jpg&imgrefurl=http://www.home-food-processing.com/browseproducts/-32164-Grape-Spiral-for-Food-Strainer.html&usg=__J2qQqd0XJISZEnRDixJWRE4OYMs=&h=300&w=300&sz=25&hl=en&start=14&tbnid=Iv6wrnzVaAWHgM:&tbnh=116&tbnw=116&prev=/images%3Fq%3Dberry%2Bscreen%26hl%3Den%26 | 10:37 |
fenn | duhh sorry about the linkspam | 10:37 |
fenn | http://www.enasco.com/product/C16122N | 10:38 |
fenn | http://www.home-food-processing.com/browseproducts/-32164-Grape-Spiral-for-Food-Strainer.html | 10:38 |
fenn | as the algae builds up on the screen it gets scraped off and pumped out | 10:39 |
kanzure | I was thinking of blockin the sensor that signals whether or not the lis is closed on my ps2 ancd then drawing a circular patter as it spins so that I can get a clean microchannel totest with. | 10:40 |
kanzure | *blocking | 10:41 |
kanzure | *lid | 10:41 |
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kanzure | *pattern | 11:23 |
kanzure | *to test | 11:23 |
katsmeow-afk | lo | 12:04 |
katsmeow-afk | l | 12:04 |
kanzure | Cell lysis on a microfluidic CD (compact disc) http://heybryan.org/books/papers/microfluidics/Cell%20lysis%20on%20a%20microfluidic%20CD%20(compact%20disc).pdf | 12:45 |
kanzure | huh | 13:22 |
kanzure | so apparently elmer and salomé aren't in debian because of those licensing issues of opencascade | 13:22 |
kanzure | anyway, opencascade 6.3.0 has been accepted into the repos apparently. | 13:23 |
kanzure | " In contemporary phosphoramidite oligonucleotide synthesis coupling | 13:27 |
kanzure | failure rates should be 0.5% or less per coupling event. | 13:27 |
kanzure | " | 13:27 |
kanzure | " the nature of coupling | 13:27 |
kanzure | failures can be found in Temsamani et al. (1995) and Hecker and Rill (1998). | 13:27 |
kanzure | " | 13:27 |
kanzure | Hecker KH, and RL Rill 1998 Error analysis of chemically synthesized polynucleotides. | 13:30 |
kanzure | BioTechniques 24: 256-260. | 13:30 |
kanzure | "Deletions are most likely to result from incomplete capping or de-tritylation." | 13:33 |
kanzure | hm, it might be due to the reversability of the detritylation step | 13:40 |
fenn | elmer uses opencascade? | 13:41 |
kanzure | origin of impurities in oligonucleotides: http://heybryan.org/books/Biology/Origin%20of%20impurities%20in%20oligonucleotides.pdf see page 29 | 13:49 |
kanzure | with references :) | 13:49 |
kanzure | http://heybryan.org/mediawiki/index.php/DNA_synthesizer#Origin_of_oligonucleotide_impurities_and_errors | 13:52 |
kanzure | added the references. | 13:57 |
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kanzure | grated array with sharpie microfluidics: scotch tape dispenser has little spikes in a row. works nicely at scraping off parallel lines. My dispenser here has 17. | 15:32 |
kanzure | can tape remove nail polish? because it can remove layers of sharpie.. | 15:40 |
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fenn | i think heekscad might actually be useful already: http://fenn.freeshell.org/motor_mount.png | 18:31 |
transilluminator | nice. | 18:38 |
transilluminator | also, growing algae in large volumes of water is stupid | 18:41 |
transilluminator | http://heybryan.org/bioreactor/flat/ | 18:41 |
fenn | indeed | 18:42 |
fenn | i recall reading about a tube of heat-sealed plastic that was stretched out on a roof and had circulating algae fed through it | 18:42 |
fenn | it was about 6 inches thick and 50 feet long | 18:42 |
transilluminator | most papers focus on photobioreactor issues like "getting light to the algae" | 18:44 |
transilluminator | which isn't really the real issue in these things. yes, it's important, but I feel that's easy | 18:44 |
transilluminator | harvesting is more of a big deal, since the smaller buggers aren't going to just clump together naturally in some cases | 18:44 |
fenn | seems like some sort of spray or waterfall would be beneficial | 18:44 |
transilluminator | and chemicals for that are sort of expensive | 18:44 |
transilluminator | how so? | 18:44 |
fenn | for aeration and temperature reduction | 18:45 |
transilluminator | anyway- those papers on flat beds seem useful. just grow it in a few cm of water, but a large surface area | 18:45 |
* fenn notes that none of those papers have photos of real installations | 18:45 | |
fenn | oh wait, there's one | 18:46 |
transilluminator | one mentioned an installation that was tested for 12 days in Japan | 18:46 |
fenn | looks pretty bad tho | 18:46 |
fenn | vertical flat plate = stupid | 18:47 |
transilluminator | yes, I don't know what they are thinking there | 18:47 |
transilluminator | they must be pumping it back up to the top | 18:47 |
fenn | i wish these guys spent more time making working systems and less time with fancy graphs and equations | 18:48 |
transilluminator | I demoed gnuplot for two biologists the other day and they went totally ballistic and all happy-feely about how awesome 'splot' was. | 18:48 |
fenn | huh? | 18:49 |
fenn | can you even rotate it in 3d? | 18:49 |
transilluminator | yes | 18:49 |
fenn | does it output SVG? :) | 18:50 |
transilluminator | it outputs dxf, does that count? | 18:51 |
fenn | apparently it does | 18:51 |
fenn | set terminal svg | 18:51 |
fenn | set output "$file.si.svg" | 18:51 |
fenn | replot | 18:51 |
transilluminator | hm, I did "set term" and looked at the list and didn't see svg there, although the interwebs tell me it does | 18:51 |
fenn | have to press return to see the full list | 18:53 |
fenn | it works! | 18:56 |
fenn | that certainly isn't x^2 though | 18:56 |
transilluminator | set xrange [0:1] | 18:57 |
fenn | it doesnt want to write the file until i exit | 19:00 |
transilluminator | there's another way to write files. | 19:00 |
transilluminator | how is it that you are only now just discovering gnuplot? | 19:01 |
fenn | i've used it before, i just forget all the syntax every time | 19:01 |
transilluminator | well, no, not the syntax, I can't penalize you for that | 19:01 |
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gene_ | kanzure find me a tiny 640x480 display | 19:57 |
kanzure | isn't this what Google is for? | 19:58 |
gene_ | http://www.emagin.com/products/OLEDMD/OLED_microdisplays.php | 19:58 |
gene_ | yeah | 19:58 |
gene_ | are you busy? | 19:58 |
gene_ | dang, dmd displays are about to get cheap | 19:59 |
gene_ | so here's the deal, you find a display with that rez that is cheap, and find a way to hook it up to say, my laptop, I make you a case for it | 20:00 |
kanzure | a case? | 20:01 |
gene_ | http://www.tradeeasy.com/supplier/3690/products/p999348/usmart.html | 20:01 |
gene_ | ugh that's a bit big | 20:01 |
gene_ | yeah | 20:01 |
gene_ | IE the part that holds it on your head | 20:01 |
gene_ | and optics | 20:02 |
katsmeow | MCM was selling 5 and 7 inch led color screen for cheap a while back, set in unside for an ntsc feed, had speakers too, ran on 12vdc | 20:02 |
gene_ | I have a cheap about 5 inch LCD screen | 20:02 |
gene_ | it's heavy and would cause head strain | 20:02 |
gene_ | used on underwater robot control system | 20:03 |
* katsmeow nods | 20:03 | |
gene_ | http://www.tradeeasy.com/supplier/144391/products/p939908/keychain-digital-photo-frame.html | 20:03 |
gene_ | 240X160 | 20:03 |
gene_ | compromise | 20:03 |
kanzure | I suggest finding something on ebay from a well-supported, but broken cell phone | 20:04 |
gene_ | good plan | 20:04 |
kanzure | I wonder if people put up cell phones on ebay once their minutes are used up | 20:05 |
kanzure | just because they don't know any better. | 20:05 |
gene_ | that 240x160 looks tempting | 20:05 |
gene_ | my phone has 176*x220 | 20:06 |
gene_ | slightly more pixels | 20:06 |
gene_ | but wider screen | 20:06 |
gene_ | optics | 20:07 |
gene_ | oh shit optics | 20:07 |
gene_ | http://www.engadget.com/2008/10/23/emagins-sxga-oled-xl-microdisplay-perfect-for-night-vision-gog/ | 20:07 |
katsmeow | sorry Hal, i cannot do that | 20:07 |
gene_ | that weird shapes gonna be hard to make | 20:07 |
kanzure | gene_: how about you put this on a wiki page | 20:08 |
kanzure | http://heybryan.org/mediawiki/index.php/Wearables | 20:08 |
gene_ | sure | 20:08 |
kanzure | since we've already talked about this before in here, and all of those notes have to be retrieved anyway | 20:08 |
kanzure | so might as well put it in one place | 20:08 |
gene_ | v | 20:12 |
gene_ | http://www.branders.com/s/personalized-clock-frame-digital-photo-73337.html | 20:12 |
gene_ | 12 for about $40? | 20:12 |
gene_ | ugh | 20:12 |
gene_ | http://www.promopeddler.com/03-64/square-portable-mini-digital-photo-keychain-holds-qqp191768.htm | 20:13 |
gene_ | damn that minimum order | 20:13 |
gene_ | http://www.farfromboring.com/Promotional-Product/Picture-Frames/Digital-Picture-Frames/DIGITAL-PHOTO-CLOCK-FRAME-MPD-9800-108186.html | 20:14 |
gene_ | request a sample ;) | 20:14 |
fenn | gene_: what's this about CNC eh? | 20:14 |
fenn | the openmoko freerunner has a tiny 640x480 screen | 20:15 |
gene_ | how much? | 20:15 |
gene_ | is it FREE! | 20:15 |
gene_ | how wide is the screen? | 20:16 |
kanzure | http://pipes.yahoo.com/pipes/pipe.info?_id=9c440036b120679b4498fcaa67200718 Google Scholar RSS/email copy | 20:17 |
kanzure | http://www.dapper.net/dapp-howto-use.php?dappName=PapersGoogleScholar | 20:17 |
kanzure | http://www.dapper.net/dapp-howto-use.php?dappName=GoogleScholarResultsExtraction | 20:17 |
fenn | gene_: like 3 inches | 20:17 |
kanzure | http://pipes.yahoo.com/pipes/pipe.info?_id=9c440036b120679b4498fcaa67200718 | 20:17 |
gene_ | that's HUGE! | 20:17 |
kanzure | http://davide.eynard.it/?p=35 "I wrote this little perl script which requires two parameters: the name of the author and the paper title. It then queries Scholar and returns the number of citations for that paper. Quick and easy!" | 20:17 |
fenn | 65mm | 20:18 |
gene_ | yeah that's quite bulky | 20:19 |
fenn | 43mm x 58mm display aarea | 20:19 |
fenn | what did you expect? | 20:19 |
gene_ | http://www.holoeyesystems.com/lcos_microdisplay_specifications.html | 20:19 |
gene_ | expensive yet awesome | 20:20 |
* kanzure is trying to figure out if there are any 'google scholar' mashups for trends analysis etc. | 20:20 | |
* fenn is trying to figure out if gene is a retard | 20:20 | |
fenn | gene_: did you get a quote? | 20:21 |
kanzure | http://userscripts.org/scripts/show/836 "saved searches" for google scholar | 20:21 |
gene_ | no | 20:22 |
gene_ | I should though | 20:22 |
kanzure | http://userscripts.org/scripts/show/12692 "I'm addicted to 'starring' items in google reader, so I created a script that adds this option to scholar. just search the site and click on the star if a result interests you. Hit 'Starred' next to the search button to see all starred results." | 20:22 |
kanzure | what's the point of that? | 20:22 |
kanzure | useful: http://userscripts.org/scripts/show/41663 ScienceDirect popup-blocker Opens PDFs in the same window, not a pop-up | 20:24 |
gene_ | you know I wonder if you can stack those tiny digital picture frames together and offset them just a bit to get a higher res display | 20:30 |
fenn | emagin = $1500 for two displays | 20:30 |
gene_ | or vibrate them like they do with dmds | 20:31 |
fenn | dmd's dont vibrate do they? | 20:32 |
fenn | not the pixel array | 20:32 |
gene_ | some do | 20:33 |
fenn | not the pixel grid i mean | 20:33 |
fenn | the laser galvo's yes | 20:33 |
gene_ | yes the whole pixel array back and forth | 20:33 |
kanzure | what the fuck | 20:33 |
kanzure | http://en.wikipedia.org/wiki/Google_Scholar | 20:33 |
kanzure | AutoScholar, an automated interface to Google Scholar for grabbing papers written in perl. | 20:33 |
kanzure | http://heybryan.org/projects/autoscholar/ | 20:33 |
kanzure | huh | 20:33 |
gene_ | double the amount of pixels | 20:33 |
gene_ | you really have to see it though to understand it | 20:33 |
fenn | see what? | 20:34 |
kanzure | http://www.scitopics.com/ | 20:34 |
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gene_ | http://cgi.ebay.com/SONY-ELECTRONIC-VIEW-FINDER-DXF-601-LENS-CAMERA-PRO_W0QQitemZ350175018595QQcmdZViewItemQQptZLH_DefaultDomain_0?hash=item350175018595&_trksid=p3286.c0.m14&_trkparms=72%3A1205|66%3A2|65%3A12|39%3A2|240%3A1318 | 20:54 |
gene_ | electronic viewfinder | 20:54 |
kanzure | hey, smari's getting slashdotted: http://slashdot.org/firehose.pl?op=view&id=3676189 | 21:11 |
kanzure | http://smari.yaxic.org/blag/2009/03/06/microsoft-skull-fucks-icelands-economy-contracts-syphilis/ | 21:11 |
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kanzure | For the witnessing to be legal the parties generally either have to work for some | 21:48 |
kanzure | third party whom they've signed right over to (eg IBM). Or else they have some | 21:48 |
kanzure | erm. | 21:48 |
kanzure | http://uncyclopedia.wikia.com/wiki/UnNews:Recession_now_affecting_time_itself%3B_one_hour_lost_Sunday | 21:48 |
fenn | sad but true | 21:51 |
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fenn | wow a whole day with no diybio | 22:26 |
kanzure | I've had no interesting emails this weekend :( | 22:38 |
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gene_ | would you like me to change that kanzure? | 23:10 |
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