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| kanzure | reverse outsourcing: http://news.slashdot.org/comments.pl?sid=1276705&cid=28417591 | 07:32 |
|---|---|---|
| genehacker | huh? | 07:35 |
| genehacker | I can't wait till we start robosourcing or space sourcing | 07:36 |
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| kanzure | fenn: in the case of sinusoidal alternating current going through an inductor, there's this variable Ip which represents the amplitude of the current (current is in units of amperes) | 11:40 |
| kanzure | so .. is an "ampere" unit supposed to have a variable attribute called "amplitude" ? | 11:40 |
| kanzure | amps.amplitude ? | 11:40 |
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| kanzure | er, where does this come from? | 11:45 |
| kanzure | http://physics.nist.gov/cuu/Units/i/SubwayDiagram.gif | 11:45 |
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| ybit | kanzure: aren't you a little afraid someone is going to take legal action against adl.serveftp.org? | 12:42 |
| ybit | perhaps an anonymous p2p solution would be better? slower but reduces the chances of prosecution | 12:42 |
| kanzure | uh? no? | 12:43 |
| kanzure | why would they take legal action? | 12:43 |
| ybit | because of copyright | 12:44 |
| ybit | though i suppose maybe something like what the music blogs are doing would work: a little notice saying we will take down the papers if asked by the publisher, and then just put it on an anonymous p2p server...?... | 12:45 |
| kanzure | music blogs? | 12:45 |
| kanzure | maybe. | 12:46 |
| ybit | most researchers don't mind though, it's the journals which i'm slightly concerned about | 12:47 |
| ybit | s/journals/publishers | 12:47 |
| kanzure | if you want to draft a page that says so, go ahead | 12:48 |
| ybit | These papers are here for purely academic purposes. If you are a representative | 12:54 |
| ybit | is that fine? | 12:54 |
| ybit | of a Publisher, and would like to have a paper removed, please contact kanzure@gmail.com | 12:54 |
| kanzure | ok | 12:55 |
| ybit | okay, it's there | 12:55 |
| kanzure | where? | 12:55 |
| ybit | papers dir | 12:55 |
| kanzure | what is the file name? | 12:55 |
| ybit | first paper to appear | 12:56 |
| kanzure | er, what? | 12:56 |
| ybit | 1- A Notice to Publishers | 12:56 |
| kanzure | http://adl.serveftp.org/papers/1-%20A%20Notice%20to%20Publishers.txt | 12:56 |
| kanzure | ok | 12:56 |
| kanzure | woah, wtf are those .com files doing there | 12:57 |
| kanzure | fixed | 12:57 |
| genehacker | you could just use the classic, "I thought no one else could see this, it must've been hackers" defense | 13:07 |
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| xp_prg | way to go Bryan, this guy is so exposed | 14:01 |
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| ybit | http://diybio4beginners.blogspot.com/2009/06/nycs-diy-bio-group-insert-green.html :: Sunday, June 21, 2009 | 14:43 |
| ybit | "NYC's DIY BIO GROUP Insert Green Flourescent Protein Genes into DNA" | 14:43 |
| ybit | Tuesday, June 16, 2009 | 14:43 |
| ybit | NPR TODAY RADIO: Kay Aull discussing her home DNA Lab for genotyping her HFE gene | 14:43 |
| ybit | Tuesday, June 16, 2009 | 14:43 |
| ybit | http://diybio4beginners.blogspot.com/2009/06/npr-today-radio-kay-aull-discussing-her.html | 14:43 |
| kanzure | hello | 14:47 |
| kanzure | xp_prg: what? | 14:47 |
| kanzure | xp_prg: my point wasn't to expose him | 14:47 |
| kanzure | xp_prg just wanted him to be honest | 14:47 |
| xp_prg | you exposed him as being dishonest, thanks | 14:53 |
| drazak_ | anyone with half a brain can genotype a gene with half the equipment she has, you need a primer or two, and then you do pcr by hand, need a restriction enzyme, badabing badaboom, compare it to the expected results for the normal gene with the same DNA ladder | 14:56 |
| genehacker | heh one of the reasons I want to build a DNA chip synthesizer is so I can test for a whole bunch of sequences | 15:00 |
| kanzure | drazak_: you should psot that | 15:08 |
| kanzure | seriously though. you guys need to back up some of the good ideas in here from time to time :) | 15:08 |
| kanzure | otherwise they'll all think I'm just crazy or something | 15:08 |
| drazak_ | that, and you can do gel electrophoresis with half the shit the OPENBOX shit has | 15:16 |
| drazak_ | heck, I could do it with shit from my kitchen, and a hot glue gun | 15:16 |
| kanzure | hot glue gun electrophoresis? | 15:16 |
| drazak_ | 12V battery, copper wire, deep large tupperware, shallow tupperware that fits inside, hotglue it inside, pour gel, add buffer, hook up battery | 15:17 |
| kanzure | ah, well, do you have gel in your kitchen? | 15:18 |
| kanzure | drazak_: you should post . | 15:18 |
| drazak_ | well, all I'm saying is, they're making shit too fancy, you can do it pretty easy with stuff from your kitchen | 15:18 |
| drazak_ | you could probably use food grade agarose, if you properly remove the insoluble solids | 15:18 |
| drazak_ | kanzure: I'm lazy | 15:20 |
| drazak_ | the main insoluble solids you have to worry about are big enough to be caught by filter paper, but that's a whole nother mess | 15:21 |
| kanzure | you're all lazy :p | 15:30 |
| kanzure | bah | 15:30 |
| * kanzure writes some code for a super star destroyer and yells at some kids to get off his laqwn | 15:30 | |
| kanzure | *lawn | 15:30 |
| drazak_ | I might starts doing some posts on doing shit at home without buying expensive shit | 15:40 |
| genehacker | cool | 15:40 |
| genehacker | diybio sorta sucks right now | 15:40 |
| drazak_ | yeah, I noticed | 15:40 |
| drazak_ | I don't read half the emails anymore, cause they're pointless | 15:40 |
| genehacker | compared to underground chemistry at least | 15:40 |
| drazak_ | ooh | 15:41 |
| drazak_ | where's that one? | 15:41 |
| genehacker | the first rule of underground chemistry is do not talk about underground chemistry | 15:42 |
| drazak_ | rofl | 15:42 |
| genehacker | especially not roguesci | 15:42 |
| genehacker | NEVER EVER GO TO ROGUESCI | 15:42 |
| genehacker | go to sciencemadness instead | 15:42 |
| genehacker | the people in roguesci have been exposed to a bit too much mercury | 15:43 |
| drazak_ | rofl | 15:43 |
| drazak_ | have you seen the Hg beating heart? | 15:43 |
| genehacker | no really I'm serious | 15:43 |
| drazak_ | HgSO4+Fe=BEATING HEART, MAN | 15:43 |
| digitaltao | o_O mad alchemists | 15:43 |
| kanzure | oh, it's just roguesci | 15:44 |
| kanzure | rougesci has been around forever | 15:44 |
| drazak_ | I should start commenting on the safety of all of this stuff | 15:45 |
| drazak_ | "yeah, you're going to blow yourself up, that's what blows up meth labs using the red-white-blue method" | 15:45 |
| kanzure | or lack thereof | 15:45 |
| kanzure | haeh | 15:45 |
| kanzure | blah. don't listen to me and my typos. | 15:45 |
| drazak_ | ap chemistry was funny this year | 15:45 |
| drazak_ | everyone thought I was making shit up | 15:46 |
| drazak_ | the teacher professor dude was talking about HNO3 being a strong oxidizer, and I mentioned that it's what blows up meth labs, and nobody believed me, and then I started talking about meth labs and their assosciated chemical processies for 10 minutes | 15:47 |
| digitaltao | remember kids, dont buy large boxes of matches, buy the bulk small..... the striker pads are what you are after | 15:47 |
| drazak_ | I has swedish fire steel | 15:47 |
| drazak_ | I light bunsen burners with it :D | 15:47 |
| digitaltao | ahhh, those are fun to play with | 15:48 |
| genehacker | kanzure have you been to roguesci as of recently? | 15:48 |
| drazak_ | digitaltao: I know, I strike it everywhere, and people yell at me | 15:48 |
| drazak_ | and I'm like | 15:48 |
| drazak_ | yeah, the distance between it and the floor is great enough that it won't ignite the carpet | 15:49 |
| * drazak_ cringes | 15:49 | |
| kanzure | genehacker: no | 15:52 |
| kanzure | genehacker: it has been more than a year | 15:52 |
| genehacker | some of the recent post include, "hey guys I think we could totally destroy the ecosystem with these chemicals" and "hey guys lets go sterilize half the population" | 15:54 |
| genehacker | I kid you not | 15:57 |
| genehacker | that's why I don't go to roguesci | 15:58 |
| drazak_ | this just screams dangerous | 16:00 |
| genehacker | dangerous? | 16:00 |
| drazak_ | h2so4 lead chamber process | 16:00 |
| digitaltao | -made a vacuum coffee apparatus today... YAY | 16:00 |
| drazak_ | yes dangerous | 16:02 |
| genehacker | drazak know anyway I could make precursors for precursors for precursors to photolabile phosphoramidites | 16:03 |
| drazak_ | lol | 16:03 |
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| digitaltao | heh | 16:03 |
| drazak_ | and you're using KNO3 which isn't exactly safe | 16:03 |
| drazak_ | not that it's exactly dangerous | 16:03 |
| genehacker | or just photolabile phosophoramidites without having to result to using sigma aldrich | 16:03 |
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| --- Log opened Mon Jun 22 16:09:26 2009 | ||
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| -!- Irssi: #hplusroadmap: Total of 19 nicks [0 ops, 0 halfops, 0 voices, 19 normal] | 16:09 | |
| genehacker | could you figure out how to make NPPOC-D-adenosine (N6- | 16:09 |
| genehacker | tac) -cyanoethylphosphoramidite from scratch if I gave you information on how to make it from precursors? | 16:09 |
| drazak_ | and you'd be exchanging some HSO4- and SO42- ions | 16:09 |
| drazak_ | can you give me the CAS number? | 16:09 |
| drazak_ | easier to look it up from CAS number, because naming conventions are wonky with shit like that | 16:10 |
| genehacker | what's a CAS number? | 16:13 |
| drazak_ | all molecules have a CAS number | 16:13 |
| drazak_ | http://www.commonchemistry.org/ChemicalDetail.aspx?ref=50-78-2&terms=aspirin | 16:13 |
| genehacker | are you sure? | 16:13 |
| drazak_ | yeah | 16:13 |
| drazak_ | well, all well known molecules | 16:13 |
| genehacker | this isn't a well know molecule | 16:13 |
| drazak_ | oh :P | 16:14 |
| drazak_ | do you know the structure of it? | 16:14 |
| drazak_ | cause I sure am brainfarting on it | 16:14 |
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| drazak_ | sorry, had to go put gas in the generator | 16:33 |
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| drazak_ | genehacker: hm | 16:34 |
| drazak_ | that's a toughy | 16:34 |
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| kanzure | lag: 141 (??) | 17:54 |
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| drazak_ | kanzure: phail | 17:58 |
| genehacker | in a pail? | 17:58 |
| drazak_ | aye | 17:59 |
| genehacker | what'd I miss? | 17:59 |
| drazak_ | nothing | 17:59 |
| genehacker | ok | 17:59 |
| kanzure | http://www.opencircuits.com/SFE_Footprint_Library_Eagle | 18:03 |
| kanzure | sparkfun eagle library of their components | 18:04 |
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| kanzure | Deby L. Cassill, Skye Brown, Devon Swick and George Yanev. Polyphasic | 19:22 |
| kanzure | Wake/Sleep Episodes in the Fire Ant, Solenopsis Invicta. Journal of Insect | 19:22 |
| kanzure | Behavior, 2009, Volume 22, Number 4: 313-323. | 19:22 |
| kanzure | http://fritzing.org/learning/tutorials/creating-custom-parts/ | 19:41 |
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| kanzure | http://fritzing.org/learning/tutorials/creating-custom-parts/parts-editor/ | 19:43 |
| fenn | "major tom" by peter schilling | 20:27 |
| kanzure | your other music sucked | 20:42 |
| fenn | racist! | 20:45 |
| kanzure | todo: build giant borg cube vessel | 20:48 |
| kanzure | somebody from diybio emailed me off-list from diybio | 20:55 |
| kanzure | and he thought that I was "in charge" of diybio | 20:55 |
| kanzure | :-/ | 20:55 |
| kanzure | No machine can do my job as resentfully as I can. http://www.theonion.com/content/opinion/no_machine_can_do_my_job_as?utm_source=b-section | 21:09 |
| kanzure | fenn: http://192.168.1.104/~bbishop/docs/miserably.txt | 21:11 |
| ybit | "Thank you for visiting our website. I have attached our datasheet and some background info on the mySEM. Price starts at ~$100K depending on options." | 21:46 |
| ybit | wowzers | 21:46 |
| ybit | knock off two zeros and i might be interested | 21:49 |
| ybit | has anyone found an SEM for 1-10k? | 21:49 |
| ybit | mySEM looks wonderful, minus the pricetag | 21:50 |
| ybit | and the dependency on windows | 21:50 |
| ybit | it came out of a darpa fund to create a low-cost sem machine | 21:51 |
| kanzure | mySEM? | 21:54 |
| kanzure | I have not seen a cheap SEM being sold | 21:54 |
| kanzure | however I really do recommend the AFM project at least | 21:54 |
| kanzure | with the CAD models | 21:54 |
| ybit | the afm project is just the tip though, correct? | 21:55 |
| ybit | couple it with the "full control of ultrasonic motor with microcontroller" paper and you might be able to create one though | 21:55 |
| kanzure | ybit: no | 21:57 |
| ybit | go on.. | 21:57 |
| kanzure | ybit: it included the CAD models for various plastic brick thingies | 21:57 |
| ybit | link please? | 21:57 |
| kanzure | as well as a parts list and links to where to order the parts | 21:57 |
| kanzure | er | 21:57 |
| kanzure | it's in the diybio archives for now | 21:57 |
| ybit | maybe we are thinking of diff. papers | 21:57 |
| ybit | hmm.. | 21:57 |
| kanzure | there was also a paper with it called "print your AFM" | 21:57 |
| ybit | kanzure: i don't have afm.zip | 22:01 |
| ybit | oh wait.. | 22:01 |
| ybit | right, don't have it. probably not linked from anywhere on heybryan | 22:03 |
| ybit | so anyway, this is what i had looked at | 22:04 |
| ybit | http://web.archive.org/web/20080106164837/http://www.biophysik.physik.uni-muenchen.de/PlasticAFM/ | 22:04 |
| ybit | what you had linked to | 22:05 |
| ybit | "How to build a Plastic-AFM head" | 22:05 |
| ybit | just saw the title and ignored the rest, seems there's more info then the title suggests | 22:06 |
| ybit | btw, thanks fenn for pointing out the misspelled ssh address | 22:23 |
| ybit | http://adl.serveftp.org/papers/POSaM_a_fast_flexible_open-source_inkjet_oligonucleotide_synthesizer_and_microarrayer.pdf | 22:24 |
| fenn | yoi | 22:26 |
| fenn | i dont get what's so impressive about printing plastic to hold all the interesting tech bits | 22:27 |
| ybit | kanzure: mind putting afm.zip on adl.serveftp? | 22:28 |
| fenn | he's asleep | 22:29 |
| fenn | and the connection here is fubared | 22:29 |
| * ybit doesn't know enough about SEM to know, but it just seems that you could use a cathode ray in construction of a low-cost SEM | 23:38 | |
| ybit | you're just beaming focused electrons at something and measuring which ones bounce back or go through the surface | 23:38 |
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