| --- Day changed Sat Jun 05 2010 | ||
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| fenn | interesting idea, pepakura repstrap http://forums.reprap.org/read.php?152,46623 | 05:20 |
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| kanzure | fenn: do you read the reprap forums frequently? | 08:53 |
| fenn | never | 09:21 |
| fenn | wiggle stereoscopy: http://www.stereomaker.net/sample/ani/ani_e.htm | 09:22 |
| fenn | if it doesn't make sense, you are probably a robot | 09:22 |
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| kanzure | http://openpcr.org/2010/06/why-we-built-openpcr/ | 09:53 |
| kanzure | i find it weird that tito is still trying to sell $200 pieces of acrylic | 09:55 |
| kanzure | apparently less than 10 people have purchased his gel box | 09:55 |
| kanzure | (no surprise there) | 09:55 |
| kanzure | "corporate anthropology" | 09:58 |
| kanzure | i've been looking over http://crisiscommons.org/ and http://oilreporter.org/ | 09:58 |
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| kanzure | hi Roy78 | 10:27 |
| kanzure | hi Yocttar | 10:27 |
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| Yocttar | Heya =) | 10:45 |
| Yocttar | whats better nerd or geek? :D | 10:46 |
| splicer | nerd... I don't understand the preoccupation with the fucking gelboxes | 10:46 |
| Yocttar | what do you mean? which preoccupation? | 10:48 |
| Yocttar | btw, realy thanks alot kanzure for referencing me over here, was looking for something of this "prison break" core idea kind :) | 10:50 |
| Alystair | gelbox? | 10:54 |
| splicer | it's the forever discussion on diybio | 10:55 |
| * Alystair should really join the mailing list | 10:56 | |
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| kanzure | Alystair: http://groups.google.com/group/diybio | 11:00 |
| kanzure | re: biocurious: http://huff.to/crJuUh | 11:18 |
| kanzure | which redirects to http://www.huffingtonpost.com/wires/2010/05/27/biocurious-the-bay-area-b_ws_592862.html | 11:18 |
| kanzure | which is just a front for http://fora.tv/2010/05/23/BioCurious_The_Bay_Area_Biology_Collaborative_Lab_Space | 11:18 |
| kanzure | gah i wish links didn't suck so much | 11:18 |
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| Alystair | beat you to it | 11:35 |
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| splicer | (looking at Titos mullet I wonder who the first with a mohawk will be) | 12:20 |
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| kanzure | hello phyrk | 15:26 |
| phryk | Hi there | 15:26 |
| kanzure | what's up? | 15:26 |
| phryk | Not much ybit told me to join ;P | 15:26 |
| phryk | What exactly is hplusroadmap? | 15:27 |
| ybit | !log | 15:27 |
| ybit | hrm :) | 15:27 |
| kanzure | hplusroadmap: You will never find a more wretched hive of scum and villainy on the internet. You must be cautious. | 15:28 |
| ybit | http://designfiles.org/irclogs.txt | 15:28 |
| kanzure | this is the one that is kept updated: http://gnusha.org/irclogs.txt | 15:29 |
| ybit | gracias | 15:29 |
| phryk | kanzure: That kinda sounds like /b/... | 15:30 |
| kanzure | http://letsfreckle.com/ sounds an awful lot like http://rescuetime.com/ | 15:33 |
| nsh | unicode ellipses look really stupid in fixed-width fonts | 15:33 |
| kanzure | phryk: we focus on transhuman tech, singularities or accelerating trends, and uh | 15:34 |
| kanzure | something else | 15:34 |
| nsh | comfy chairs | 15:36 |
| phryk | Ah nice | 15:36 |
| phryk | Like Aubrey deGrey or whatever his name was? | 15:36 |
| nsh | he's actually our telepresence hairbot | 15:37 |
| kanzure | "Love the streamed sets because they don't interrupt your workflow. It's like being at a coding dance party; for hours." | 15:37 |
| phryk | Okay^^ | 15:37 |
| kanzure | http://trancearoundtheworld.com/ | 15:38 |
| phryk | coding dance party sounds like some real fun | 15:38 |
| phryk | Mh, not really my type of music | 15:39 |
| phryk | Only stream i (seldomly) listen to is ... dead as i just had to witness now :/ | 15:39 |
| phryk | Poor uppercut radio :'( | 15:40 |
| genehacker | anyone here buy stuff from mcmaster-carr? | 15:42 |
| genehacker | oh shoot where's the streams? | 15:43 |
| kanzure | genehacker: here's some different streams: http://designfiles.org/~bryan/music/url.txt | 15:47 |
| kanzure | and stuff i've found that i like is listed here: http://designfiles.org/~bryan/music/ | 15:47 |
| kanzure | right now i'm on http://www.di.fm/mp3/vocaltrance.pls (doing some facebook app dev bullshit) | 15:48 |
| genehacker | oh I thought you were at hplus | 15:49 |
| kanzure | wait did i miss my flight? | 15:49 |
| kanzure | i thought the event started on the 12th | 15:49 |
| genehacker | I am having temporal awareness problems | 15:49 |
| genehacker | anyway can I visit the space tomorrow | 15:50 |
| kanzure | sure, do you need a ride? | 15:50 |
| genehacker | yeah | 15:51 |
| kanzure | alright | 15:51 |
| phryk | You guys have a dedicated hackerspace? | 16:11 |
| genehacker | #austinhackerspace for more info | 16:12 |
| phryk | That's a creative name :P | 16:13 |
| phryk | Ooh | 16:15 |
| phryk | splicer: We meet again :D | 16:15 |
| phryk | All that stuff around transhumanism, diybio and biopunk seems to be pretty small currently^^ | 16:16 |
| kanzure | small? | 16:20 |
| phryk | Yes. | 16:21 |
| phryk | You keep running into the same people over different routes^^ | 16:21 |
| phryk | I meant the community | 16:22 |
| Alystair | not many people realize change is necessary ;) | 17:48 |
| phryk | Well for life, change is always necessary. | 17:49 |
| phryk | Stagnation practically is death... | 17:50 |
| phryk | Also, I'm going to sleep now ;P | 17:50 |
| phryk | Good night ;) | 17:50 |
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| kanzure | /window 213 | 19:06 |
| kanzure | crap | 19:06 |
| kanzure | second time today :( | 19:06 |
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| genehacker | http://www.mother-of-robots.org/ | 19:28 |
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| jrayhawk | do you seriously have 213 windows open | 19:51 |
| jrayhawk | that made steve laugh | 19:52 |
| * nsh wouldn't be at all surprised | 19:53 | |
| kanzure | jrayhawk: do you even know who i am? | 19:54 |
| kanzure | jrayhawk: http://heybryan.org/shots/2008-01-15-tabs.png is common | 19:54 |
| nsh | reminds me, have you done a bookmark dump lately? | 19:55 |
| kanzure | my bookmark managers all crashed and refuse to work with me | 19:55 |
| kanzure | so i don't use them any more | 19:55 |
| kanzure | back to memorizing urls! | 19:55 |
| nsh | ah | 19:56 |
| nsh | let me know when you do a brain dump then :) | 19:56 |
| * kanzure fiddles around with http://jambool.com/ for "virtual currency" | 20:00 | |
| nsh | there any other kind? | 20:11 |
| kanzure | what? | 20:11 |
| nsh | any other kind of currency than virtual | 20:11 |
| nsh | fiat | 20:11 |
| kanzure | no | 20:11 |
| genehacker | sounds like you're making one of those facebook crack addiction simulations things... | 20:12 |
| egeste | hello | 20:12 |
| kanzure | genehacker: yep, that's exactly what i'm doing | 20:13 |
| kanzure | hi egeste | 20:13 |
| genehacker | with other people? | 20:13 |
| kanzure | ? | 20:13 |
| kanzure | what? | 20:13 |
| nsh | was reading about lycurgus of sparta, who instituted a currency made of iron coins, dipped in vinegar to become brittle and intrinsically worthless | 20:13 |
| genehacker | you said you were having a programming party earlier | 20:13 |
| nsh | http://www.e-classics.com/lycurgus.htm | 20:13 |
| nsh | to eliminate the desire to acrue wealth | 20:14 |
| genehacker | well that would eliminate the inflation problem | 20:14 |
| genehacker | unfortunately that would eliminate inflation | 20:14 |
| * nsh smiles | 20:14 | |
| nsh | yes lisa, crisotunity! | 20:15 |
| kanzure | genehacker: no that was a quote that i copied-and-pasted | 20:15 |
| kanzure | i'm doing the facebook app on my lonesome | 20:15 |
| kanzure | just a learning experience | 20:15 |
| kanzure | it will be demoed at h+ summit 2010 by andrew hessel,l though | 20:15 |
| kanzure | presumably exxon is going to dump a few million into it.. but whatever | 20:15 |
| kanzure | i don't expect that to actually happen | 20:16 |
| genehacker | so it's something more useful than facecrack? | 20:16 |
| nsh | app does what? | 20:16 |
| * nsh is considering writing a flashcard (recall aid) app for facebook | 20:16 | |
| nsh | or, more ambitiously, a collaborative rune goldberg machine construction game | 20:17 |
| kanzure | it's a virtual currency on top of http://oilreporter.org/ and http://crisiscommons.org/ - an auction site for co2 fixation-related activities | 20:17 |
| kanzure | nsh: fantastic contraption? :) or skdb? :D | 20:18 |
| nsh | simulated :) | 20:18 |
| kanzure | actually my 80/20 company might be interested in an app for collaborative 80/20 design bullshit | 20:18 |
| kanzure | on facebook | 20:18 |
| genehacker | so you can own virtual money that's as good as real money that you don't have to pay taxes on? | 20:18 |
| kanzure | as a way to market it to engineers to get kiddos interested in actually building shit | 20:18 |
| kanzure | genehacker: yep | 20:18 |
| nsh | 80/20 company? | 20:18 |
| kanzure | canadian oil companies have to pay $15/ton of CO2 in taxes | 20:19 |
| genehacker | a cad application for facebook might be cool | 20:19 |
| kanzure | nsh: structory.com-- basically it's a shopping cart frontend to skdb specifically for 80/20 modular beam components | 20:19 |
| kanzure | genehacker: or totally lame | 20:19 |
| nsh | oh | 20:19 |
| genehacker | well it would be less lame than farmville | 20:19 |
| genehacker | DESIGN A PNEUMATIC PROPORTIONAL VALVE AND GET 50 CAD COINS... | 20:20 |
| genehacker | or something like that | 20:20 |
| nsh | invite your friends to debug your Skynet defence system | 20:21 |
| genehacker | and use classic facebook app user enslavement practices, and soon you've crowd sourced design | 20:21 |
| genehacker | exactly | 20:21 |
| * nsh needs to read some good studies of wikipedian social development | 20:22 | |
| nsh | there are critical developmental phases | 20:22 |
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| nsh | steerage is all the more important for mechanical tower of babel | 20:23 |
| jrayhawk | god your server is so slow | 20:24 |
| jrayhawk | why aren't you using barthosting for that | 20:24 |
| kanzure | jrayhawk: i should movei t somewhere | 20:24 |
| kanzure | i have about 2 TB of bullshit on the server | 20:24 |
| kanzure | can i just.. ship it to you? | 20:24 |
| genehacker | time warner cable slow speed? | 20:24 |
| jrayhawk | oh i might have to convince bart to do some upgrades, then. I think we only have a terabyte on there. | 20:24 |
| kanzure | i can be convinced to only do a few hundred meg | 20:25 |
| kanzure | ok convinced | 20:25 |
| jrayhawk | I guess if you're willing to let me keep the drives, I can just shove 'em in the server. | 20:25 |
| kanzure | my upload is terrible, that's why i've never moved it anywhere | 20:25 |
| nsh | clone the disks | 20:25 |
| kanzure | yeah i should. | 20:25 |
| kanzure | blah | 20:25 |
| kanzure | jrayhawk: can i use the gnusha.org virtual server for this | 20:26 |
| genehacker | kanzure you wouldn't happen to know if the university has faster internet than you do? | 20:26 |
| kanzure | the university definitely has faster internet than i have | 20:26 |
| jrayhawk | Yeah, sure. Just let me know ahead of time if you seriously intend to fill up the 600GB we have available to you. | 20:26 |
| genehacker | really? | 20:26 |
| kanzure | jrayhawk: k | 20:26 |
| kanzure | genehacker: yeah.. check out the speeds on designfiles.org | 20:26 |
| jrayhawk | And yes, you can send me drives for me to deal with. | 20:27 |
| genehacker | do you have wood and drills in the hacker space then? | 20:27 |
| kanzure | lots of wood | 20:27 |
| kanzure | lots and lots of wood | 20:27 |
| kanzure | also lots of dust | 20:27 |
| kanzure | but that's unrelated :) | 20:27 |
| genehacker | I'm making an antenna mount then | 20:27 |
| genehacker | dust is fine too | 20:27 |
| genehacker | especially sawdust, we could try making a boat out of pykrete... | 20:28 |
| jrayhawk | Kanzure: you've seen newsbeauter, right? | 20:30 |
| jrayhawk | err newsbeuter | 20:30 |
| kanzure | no. /me checks | 20:31 |
| jrayhawk | It is the mutt of RSS readers. | 20:32 |
| kanzure | hm | 20:32 |
| kanzure | i need an automatic rss download script thingy so that i don't have to actually use twitter | 20:32 |
| kanzure | i.e. because they delete their contents after 24 hours or something | 20:32 |
| jrayhawk | More optimally you should be using rss2email along with sup or notmuch | 20:32 |
| kanzure | my inbox is a bullshit free zone | 20:35 |
| kanzure | well, actually.. | 20:35 |
| jrayhawk | Well, that's why you use a sieve system to classify your mail into folders (if imap) or tags (if local storage) | 20:38 |
| jrayhawk | I'm a little alarmed you don't do that already. | 20:39 |
| kanzure | what? | 20:39 |
| kanzure | i was making a witty comment | 20:40 |
| jrayhawk | oh okay | 20:40 |
| kanzure | i use tags and folders | 20:40 |
| jrayhawk | tank goodness | 20:40 |
| kanzure | my inbox is still 68,862 unread messages however | 20:40 |
| jrayhawk | golly | 20:40 |
| kanzure | however, according to the gmail.com interface my entire mail collectio nis 210,847 | 20:41 |
| kanzure | *collection is | 20:41 |
| InkBlob | 68,000 unread? do you have a fan mailing list or something? | 20:41 |
| kanzure | (also, i'm lagging today and am having rtouble typing) | 20:41 |
| kanzure | considering 68k unread, and 210k total, i'd say that's pretty good | 20:41 |
| InkBlob | i read every message sent to me. wuw kanzure. | 20:42 |
| kanzure | jrayhawk: have you seen this? http://designfiles.org/~bryan/meetlog/meetlog.txt | 20:42 |
| kanzure | InkBlob: so do i. a lot of it is not personally addressed to me | 20:42 |
| jrayhawk | yes, and i am very pleased about your religious documentation of having talked to BUTT_PORBLEMS | 20:43 |
| InkBlob | ow | 20:43 |
| kanzure | jrayhawk: you're welcome | 20:43 |
| kanzure | InkBlob: you're also welcome to send me fan mail | 20:43 |
| kanzure | you know, if you feel like it | 20:43 |
| * nsh offers to send a single peanut in an envelope | 20:44 | |
| kanzure | ? | 20:44 |
| kanzure | are you calling me a nut | 20:44 |
| kanzure | :P | 20:44 |
| nsh | no, i just have a bunch of peanuts :) | 20:44 |
| kanzure | then by all means | 20:44 |
| nsh | more seriously though | 20:44 |
| nsh | if you had a million SD cards | 20:44 |
| nsh | say 2 gig | 20:44 |
| nsh | and you could send them to a million people who would look at their contents | 20:45 |
| nsh | what would you send? | 20:45 |
| nsh | i might ask this on reddit or something | 20:45 |
| genehacker | you should | 20:45 |
| jrayhawk | goatse | 20:45 |
| kanzure | a trojan? | 20:45 |
| nsh | i wonder what a million SD cards costs at price | 20:45 |
| kanzure | probably peanuts | 20:45 |
| kanzure | speaking of which.. | 20:45 |
| * nsh chews | 20:46 | |
| jrayhawk | do not chew on goatse that is gross you are gross | 20:46 |
| nsh | hey, it's palliative chewing | 20:47 |
| kanzure | i like how our first ideas were goatse and a trojan | 20:47 |
| nsh | revealing | 20:49 |
| nsh | damn, that last mouthful of peanuts tasted amazing | 20:49 |
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| kanzure | hi GreydonSquare | 20:57 |
| kanzure | hi jumpboy11j | 20:57 |
| jumpboy11j | hi | 20:58 |
| jumpboy11j | any discussion going on here? | 20:58 |
| * jumpboy11j doesnt want to interrupt any discussion that may be going on | 20:58 | |
| kanzure | only about world domination and cybernetics | 20:58 |
| kanzure | what's up? | 20:58 |
| GreydonSquare | Hey Kanzure, sorry i was over in #newmars | 20:58 |
| jumpboy11j | eh not much | 20:59 |
| GreydonSquare | i love both topics | 20:59 |
| jumpboy11j | you? | 20:59 |
| GreydonSquare | lol | 20:59 |
| kanzure | ah, the mars society | 20:59 |
| jumpboy11j | yeah | 20:59 |
| GreydonSquare | yup | 20:59 |
| jumpboy11j | although more New Mars than Mars Society | 20:59 |
| jumpboy11j | even though NM is TMS forum | 20:59 |
| kanzure | did you ever see openvirgle? | 20:59 |
| kanzure | an open source go at the virgle project? | 20:59 |
| jumpboy11j | no | 20:59 |
| kanzure | http://openvirgle.net/ | 21:00 |
| kanzure | kinda related to http://openmanufacturing.org/ | 21:00 |
| kanzure | http://www.emcore.com/assets/photovoltaics/Paper_Navid_9-22-00.pdf | 21:03 |
| kanzure | beep bloop | 21:08 |
| jumpboy11j | doesn't look like we're talking about money anymore | 21:08 |
| kanzure | fenn: how goes the goo squirter? | 21:09 |
| jumpboy11j | how does resource allocation in the scientific sense sound? | 21:09 |
| jumpboy11j | IE production and distribution but not money | 21:09 |
| kanzure | you know, i really have too many irc channels open for me to split off conversations into yet another channel :) | 21:10 |
| jumpboy11j | haha | 21:10 |
| jumpboy11j | okay | 21:11 |
| kanzure | but josh is welcome to come back in here :P | 21:11 |
| jumpboy11j | haha | 21:11 |
| jumpboy11j | so you're a transhumanist? | 21:11 |
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| kanzure | yeah, sure | 21:11 |
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| jumpboy11j | I might or might not call myself one | 21:12 |
| kanzure | hi jasonwohlfahrt | 21:12 |
| jumpboy11j | except I'm not exactly sure what transhumanism is | 21:12 |
| kanzure | basically it's the idea of augmenting, upgrading, and getting rid of your body (this is the minimal definition i guess) | 21:12 |
| kanzure | well, not getting rid necessarily | 21:12 |
| jumpboy11j | might be amalgated into a larger whole | 21:13 |
| kanzure | primarily it's a movement of people who like to thin kabout the future and don't do much | 21:13 |
| jumpboy11j | but that's inevitable with the progression of progress isnt it? | 21:13 |
| genehacker | not necessarily | 21:13 |
| jumpboy11j | how so? | 21:13 |
| jumpboy11j | if technology keeps advancing, that is the clear next step | 21:14 |
| kanzure | there's this concept of "recursive self-improvement" floating around, but nobody has nailed down a usable quantification of it | 21:14 |
| genehacker | what if we develop nuclear bombs that anyone can make in their basement? | 21:14 |
| jumpboy11j | well that would be antithetical to progress if they were used | 21:15 |
| jumpboy11j | that's an existential threat | 21:15 |
| genehacker | what scares me is someone releasing smallpox | 21:15 |
| genehacker | north korea might have smallpox stocks | 21:15 |
| jumpboy11j | anyone might | 21:16 |
| lepton | All good reasons to spread civilization(s) beyond the earth | 21:16 |
| jumpboy11j | hell I'm sure the US does | 21:16 |
| jumpboy11j | yeah | 21:16 |
| kanzure | lepton: nobody understands that :( | 21:16 |
| genehacker | the US does | 21:16 |
| lepton | I'm excited for SpaceX this week | 21:16 |
| jumpboy11j | they launched already | 21:16 |
| lepton | I've got a friend working with them | 21:16 |
| genehacker | me too | 21:16 |
| lepton | Successfully got into orbit | 21:16 |
| kanzure | leptoi'd be interested in figuring out a way to convince people that we need to engineer reliability into our civilization | 21:16 |
| genehacker | we really do need to expand beyond earth | 21:16 |
| jumpboy11j | definitely | 21:16 |
| genehacker | first define reliability | 21:17 |
| genehacker | certainly you don't mean a factor we use when figuring out how long a part will last | 21:17 |
| kanzure | there's a lot of quantifications of reliability | 21:17 |
| kanzure | look into reliability theory | 21:17 |
| kanzure | hell even the basic saying "don't put all of your eggs in one basket" | 21:17 |
| lepton | Recently I've been trying to appreal the techo-utopian idealism of the 50's that a lot of people have in mind | 21:18 |
| genehacker | that's redundancy | 21:18 |
| lepton | reminding them that finding the prospect of space travel as silly is a recent social developtment | 21:18 |
| kanzure | yes redundancy is one part of reliability | 21:18 |
| jumpboy11j | also simplicity | 21:18 |
| kanzure | yep | 21:18 |
| jumpboy11j | but my question is still | 21:18 |
| genehacker | somethings cannot be simplified | 21:19 |
| kanzure | there are four main points as i see it: redundancy, simplicity, transparency, and uh | 21:19 |
| kanzure | crap why do i always forget the other one? | 21:19 |
| kanzure | http://en.wikipedia.org/wiki/Single_point_of_failure | 21:19 |
| kanzure | diversity :) | 21:19 |
| jumpboy11j | aren't simplicity and diversity antithetical to some extent? | 21:19 |
| kanzure | well, there's simplicity in certain forms of emergence | 21:20 |
| genehacker | heh where's that ghost in the shell quote about diversity... | 21:20 |
| kanzure | whereas under neath that layer of emergence (or "emergent order") there's actually really complex things happening | 21:20 |
| lepton | A fractal is a good example of both simplicity and diversity | 21:20 |
| lepton | a mandelbrot of such | 21:21 |
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| genehacker | "If we all reacted the same way, we'd be predictable, and there's always more than one way to view a situation. What's true for the group is also true for the individual. It's simple: overspecialize, and you breed in weakness. It's slow death." | 21:21 |
| genehacker | ah that one | 21:21 |
| jumpboy11j | still | 21:22 |
| kanzure | lepton: do you know about the seasteading insitute? | 21:22 |
| kanzure | *institute | 21:22 |
| jumpboy11j | how is transition different from any other form of futurism or progress | 21:22 |
| jumpboy11j | or even consumerism? | 21:22 |
| kanzure | what? | 21:22 |
| kanzure | "how is transition" ? what is "transition"? | 21:22 |
| jumpboy11j | sorry | 21:23 |
| jumpboy11j | its late | 21:23 |
| jumpboy11j | transhumanism | 21:23 |
| genehacker | this is sounding like philosophy | 21:23 |
| kanzure | EROR ABORT | 21:23 |
| jumpboy11j | I just want to get a handle on what transhumanism is | 21:23 |
| kanzure | ERROR | 21:23 |
| kanzure | god damn it | 21:23 |
| kanzure | jumpboy11j: http://heybryan.org/transhumanism_def.html read the part in green at the top maybe :)" | 21:23 |
| kanzure | without the quote mark blah | 21:23 |
| jumpboy11j | thanks | 21:24 |
| lepton | Yeah, I know of seasteading | 21:24 |
| lepton | I like the idea | 21:24 |
| InkBlob | discuss most humanly possible way to extend your lifespan with today's tehnology. | 21:24 |
| lepton | I like it most in the asteroid belt | 21:24 |
| InkBlob | cell printing? | 21:24 |
| jumpboy11j | does uploading onto a computer count? | 21:25 |
| kanzure | InkBlob: did you read the papers yet? | 21:25 |
| InkBlob | i didn't know it existed. | 21:25 |
| jumpboy11j | well not yet | 21:25 |
| kanzure | lepton: i think that petri friedman's idea is nice and all.. i like the idea of letting a thousand flowers bloom for different ways of doing civilization | 21:25 |
| kanzure | and having a "civilization seed" lets that happen :) | 21:25 |
| kanzure | lepton: more on that is here: http://groups.google.com/group/openmanufacturing/msg/e4c375acce772250 | 21:25 |
| kanzure | jumpboy11j: btw, the papers that i just mentioned to InkBlob are over here: http://designfiles.org/papers/longevity/ | 21:26 |
| InkBlob | no kanzure not yet, but i will definetly go knee deep into it. right now, i'm trying to make cash. | 21:26 |
| InkBlob | yup that link is in my list. | 21:27 |
| kanzure | InkBlob: if i were you, i would focus on the blood-based stem cell therapies for longevity research | 21:27 |
| nsh | don't try and make cash, for that is impossible, only try to realise the truth, that there is no cash | 21:27 |
| kanzure | i.e. blood-based therapies i mean | 21:27 |
| InkBlob | yes, stem cell therapy. i thought that was the most plausible topic. | 21:27 |
| kanzure | nsh: :) | 21:27 |
| InkBlob | blood based. got it. | 21:27 |
| kanzure | nsh: actually i've kind of always imagined you looking like that bald kid | 21:28 |
| InkBlob | is there anyother kind though? | 21:28 |
| * nsh smiles | 21:28 | |
| * kanzure steals nsh's spoon | 21:28 | |
| nsh | aerosol stem-cell therapy would be interesting | 21:29 |
| kanzure | good thing it doesn't exist huh | 21:29 |
| nsh | uite | 21:29 |
| nsh | *quite | 21:29 |
| kanzure | some jerkwads were selling oxytocin sprays a while back | 21:29 |
| kanzure | i don't recall any studies on the efficacy though | 21:29 |
| genehacker | well oxytocin microdarts would be more interesting | 21:29 |
| kanzure | oxytocin ninja darts | 21:29 |
| kanzure | gene ninjas! | 21:29 |
| InkBlob | spraying over the vody regenerative cells i read about that. it was amazing. is it even possible? | 21:30 |
| InkBlob | kewl | 21:30 |
| genehacker | if you make the dart thin enough they won't ever notice | 21:30 |
| InkBlob | v=b | 21:30 |
| kanzure | velocity equals the drag due to wind resistence? wut? | 21:30 |
| kanzure | oh typo | 21:30 |
| InkBlob | no way. i got a strand of hair in my skin once and it hurt. | 21:30 |
| kanzure | lepton: obviously i meant to say patri friedman, not petri :) | 21:31 |
| kanzure | i was thinking of mr. petri dish i guess | 21:31 |
| genehacker | well then have someone accidently bump into your target with an umbrella | 21:32 |
| lepton | ha, I didn't even notice, actually | 21:32 |
| genehacker | http://en.wikipedia.org/wiki/Georgi_Markov | 21:33 |
| kanzure | it was eating away at my soul | 21:33 |
| kanzure | egeste: are you at the space right now, and do you have documentation? | 21:33 |
| splicer | a problem with stem cell therapy is that one wants to regenerate as many cells that die... how does one regulate that? | 21:33 |
| splicer | as die | 21:33 |
| kanzure | splicer: there are certain genetic pathways that you want to trigger and manipuolate | 21:33 |
| kanzure | before the cells die | 21:33 |
| kanzure | *manipulate | 21:33 |
| splicer | yeah... if they're bot already gone | 21:34 |
| * nsh thought of petri dishes earlier when he saw the bacterial colonies growing in the wok in his (shared with 11 ablutophobic flatmates) kitchen sink | 21:34 | |
| nsh | (ablution: washing shit) | 21:34 |
| genehacker | nsh are they glowing? | 21:34 |
| kanzure | i'm going to paste some stuff on aging, hope nobody hates me for the flood | 21:34 |
| kanzure | Ageing works like this: | 21:34 |
| kanzure | 1) Electrons leak from mitochondrial Complexes I & III forming superoxide anion. | 21:34 |
| kanzure | 2) The increasing rate of leakage and the further breakdown of mitochondrial components with time under the superoxide (...and peroxynitrite & peroxide) onslaught is strongly determined by genetically controlled factors: | 21:34 |
| genehacker | you aren't supposed to wash woks | 21:34 |
| nsh | genehacker, haven't checked | 21:34 |
| kanzure | i.e., the structure of mitochondrial proteins and membranes. Hence leakage is species-specific. | 21:34 |
| kanzure | The net effect is that leakage is a mostly monotonically increasing function of time. | 21:35 |
| kanzure | 3) While the charged Superoxide Anion does not escape to the cytoplasm easily from the mitochondrial outer membrane... | 21:35 |
| kanzure | 4) Mitochondrial Superoxide Dismutase (SOD) converts Superoxide Anion into Hydrogen Peroxide. | 21:35 |
| kanzure | 5) H2O2 is neutral and easily diffuses through the mitochondrial outer membrane, entering the cytoplasm. | 21:35 |
| kanzure | 6) In the cytoplasm, peroxide prompts protein kinase C variants (incl.epsilon) to upregulate cytoplasmic NOX. | 21:35 |
| kanzure | NOX outputs O2-; and eventually via cytoplasmic SOD, H2O2; acting as a high-gain peroxide amplifier. | 21:35 |
| kanzure | THIS IS AGE-LINKED, ENDOGENOUS CHRONIC OXIDATIVE STRESS. | 21:35 |
| kanzure | 7) H2O2 is an important signalling molecule in stress signal transduction; such transduction is ramped up. | 21:35 |
| kanzure | The MAPK/ERK cascade is overactivated. | 21:35 |
| kanzure | The key stress transcription factors NF-kappaB; AP1 etc are constitutively upregulated. | 21:35 |
| kanzure | Angiotensin2 is upregulated. The PI3K-Akt signalling cascade is chronically activated. | 21:35 |
| kanzure | The transcription factors FOXO3A (and other FOXOs) are phosphorylated by Akt and translocated from the nucleus. | 21:35 |
| kanzure | Bad is similarly phosphorylated. mTOR is 'non-phosphorylatively' activated by Akt. | 21:35 |
| kanzure | Note that FOXOs, Bad, mTOR are downstream of PI3K. SIRT1 & klotho are upstream. | 21:35 |
| kanzure | 8) These factors upregulate the transcription of cytokines and a whole range of stress molecules; unleash a chronic cytokine onslaught on the cell & tissues. | 21:35 |
| kanzure | A number of runaway positive feedback loop including TLRs and RAGE is ensured. | 21:35 |
| kanzure | 9) Under the signalling from the transcribed products, massive tissue remodelling and physiological change at the cell, tissue system & organism level is enforced. | 21:35 |
| kanzure | Both Hypertrophic & Degenerative signalling abound. | 21:35 |
| kanzure | 10) ...and checkpoints are disabled promoting oncogenesis;apoptosis is aberrant | 21:35 |
| kanzure | 11) ...and stem cell niche failure - either through complete abrogation; or through enforced quiescence is the norm. | 21:35 |
| kanzure | 12) ...and mitochondrial biogeneisis is abrogated, OXPHOS is downregulated | 21:36 |
| kanzure | 13)...and cell senescence is upregulated, in part through p38MAPK | 21:36 |
| genehacker | I thought there were some findings that made oxidative stress theory look bad | 21:36 |
| genehacker | would reengineering our mitochondria help us live longer? | 21:37 |
| kanzure | aubrey wanted to move the mitochondrial genes over into the nuclear genome, but i don't remember why | 21:37 |
| genehacker | like replace our mitochondria with mitchondria from stuff that lives really long? | 21:37 |
| kanzure | anyway, oxidative stress is definitely not the only reason your body dies | 21:37 |
| genehacker | genes in the mitochondria might mutate more? | 21:38 |
| splicer | kanzure: those patways are instant.... adding the stem cells isn't | 21:38 |
| nsh | i ever tell you that story about Henry Ford? | 21:38 |
| genehacker | apply mass production to what nsh? | 21:39 |
| nsh | Henry Ford calls in his top engineers one day and says "I want you to go out to all the scrapyards in the area, and find all the ford cars, and get me a list of all the parts that have worn the least in those cars" | 21:39 |
| nsh | the engineers go out and do this, thinking maybe he's gonna try and use these parts as examples to make the other components more durable | 21:39 |
| nsh | when they report back to him, he simply says "make these parts to a lower specification from now on" | 21:40 |
| kanzure | heh | 21:40 |
| genehacker | heh | 21:40 |
| kanzure | stop reading my brains, genehacker | 21:40 |
| jrayhawk | heh | 21:40 |
| genehacker | now we're to the point where we do that before hand | 21:40 |
| nsh | i strongly suspect ageing is death by a thousand cuts, give or take. there'd be pressure to have everything start to unwind at more or less the same rate | 21:40 |
| jrayhawk | It'd be simpler to have a handful of trigger conditions that make death succict so as to help the herd. | 21:41 |
| genehacker | IE we understand how mechanical systems wear so people start making them to be cheaper and not last forever | 21:41 |
| genehacker | so the saying "they don't make them like they used to is true" | 21:42 |
| nsh | thing is, immortality has been solved already, just at a different scale level to the one we experience | 21:42 |
| jumpboy11j | bigger or smaller? | 21:42 |
| splicer | hela-cells? | 21:42 |
| genehacker | immortality is highly improbable | 21:42 |
| kanzure | "The BIG problem with traditional IV stem cell transplantation: cancer & 1st pass pulmonary filtration, is avoided with the use of these cells which are evolutionarily equipped to handle temporary niche-independence while they are mobilized in the circulation." | 21:43 |
| nsh | we want to preserve the analogue system, when it's the digital data (genes) that have already achieved it | 21:43 |
| kanzure | "I am currently trying to find ways of expanding harvested populations in vitro; with a view to reintroduction. This is difficult, because free stem cells tend to either differentiate or to transform. Additionally, the reintroduction has to be into a FULLY prepped organism, to simulate youth (UNDER 2 years of age) AND major trauma - which should start the various innate repair programs." | 21:43 |
| kanzure | "During the autologous I.V. transplant I would have to inhibit SOCS3 & upregulate thymosin beta-4 and suppress systemic constitutive NF-kappaB; among other things." | 21:43 |
| kanzure | "Use of this protocol at intervals will possibly allow maybe a 200 year lifespan; and also PAINLESS GENETIC REENGINEERING OF ADULT ORGANISMS." (ignore the caps lock) | 21:43 |
| kanzure | http://designfiles.org/papers/longevity/2010-03-23.txt | 21:43 |
| nsh | hmm | 21:44 |
| splicer | some nut? | 21:44 |
| kanzure | no :) | 21:44 |
| kanzure | although he is nutty at times- he apologizes to me for "wasting" my time | 21:44 |
| nsh | :) | 21:44 |
| nsh | i wonder what he means by "painless" | 21:45 |
| kanzure | probably "relatively"? :D | 21:45 |
| kanzure | hm | 21:45 |
| InkBlob | this stuff is crazy. | 21:45 |
| InkBlob | i'm trying to wrap my head around it. | 21:45 |
| InkBlob | i thought you were the robot-machine guy kanzure, how do you know so much about biology? | 21:46 |
| kanzure | huh? my background is more in biology than in industrial automation | 21:46 |
| kanzure | sadly i wish this was the other way around | 21:46 |
| nsh | convergence :) | 21:46 |
| genehacker | I'm more of a machine guy here | 21:47 |
| kanzure | i just get mesmorized by this stuff: | 21:47 |
| kanzure | 0EB93E6E02E5CF17 | 21:47 |
| kanzure | blah | 21:47 |
| kanzure | http://www.youtube.com/user/kanzure#grid/user/0EB93E6E02E5CF17 | 21:47 |
| InkBlob | it is? how come you talk about robot arms and stuff like that in IRC all the time. | 21:47 |
| kanzure | (my machine porn playlist) | 21:47 |
| kanzure | because i recently bought a robotic arm :) | 21:48 |
| InkBlob | well, great study. thangs. | 21:48 |
| kanzure | what? | 21:48 |
| InkBlob | Okay. | 21:48 |
| InkBlob | great study for me. good information. thanks. | 21:48 |
| kanzure | ah | 21:48 |
| genehacker | kanzure I should give you my gear porn library | 21:48 |
| kanzure | i have this song stuck in my head now: http://www.youtube.com/user/kanzure#p/c/0EB93E6E02E5CF17/37/wbDwUWGvwq4 | 21:48 |
| * nsh thinks about the new Game of Life construction -- Gemini, the first oblique spaceship | 21:49 | |
| nsh | http://www.conwaylife.com/wiki/index.php?title=Gemini | 21:49 |
| kanzure | nsh: have you seen golly? | 21:50 |
| nsh | aye, haven't tried yet | 21:50 |
| kanzure | http://golly.sf.net/ or something | 21:50 |
| genehacker | a new spaceship! | 21:50 |
| kanzure | my aussie friend has this 8 GB OSX setup running a session of golly for the past 1.5~ years | 21:50 |
| nsh | heh | 21:50 |
| nsh | on the same input? | 21:50 |
| genehacker | I have this song stuck in my head: | 21:50 |
| genehacker | http://www.youtube.com/watch?v=uJ4nmG39QXo | 21:50 |
| kanzure | yeah just one huge continuous run | 21:50 |
| kanzure | http://thewildca.com/ | 21:51 |
| kanzure | he printed it out? hah | 21:51 |
| genehacker | how do people discover these things? | 21:51 |
| genehacker | brute force? | 21:52 |
| nsh | nah, there's a whole 'technology' of game of life components and their interaction | 21:52 |
| kanzure | egeste and nsh probably will tell you something about swhack or subgenius or something | 21:52 |
| kanzure | wolfram would wave his hands and make up bullshit | 21:52 |
| kanzure | and feynman would just smirk | 21:52 |
| kanzure | (or something) | 21:52 |
| nsh | hit the bongos | 21:52 |
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| kanzure | ah, right | 21:52 |
| kanzure | yeah, he'd do that | 21:52 |
| kanzure | aww jumpboy11j left :( | 21:53 |
| genehacker | I know of the technology of game of life components | 21:53 |
| genehacker | I'm not aware of how people discover it | 21:53 |
| genehacker | I guess people engineer it? | 21:53 |
| kanzure | wolfram had this classification system for CA rules | 21:53 |
| lepton | Sounds like a good application for genetic optimization to me | 21:53 |
| genehacker | the bullshit one that got disproven? | 21:53 |
| nsh | i'd call it engineering at this point, genehacker. but i'm a layman | 21:54 |
| kanzure | nsh: pfft, yeah and i'm jesus | 21:54 |
| kanzure | wait | 21:54 |
| genehacker | engineering as opposed to cathedral building | 21:54 |
| genehacker | engineering is planning something out in advance | 21:54 |
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| nsh | see this thread: http://conwaylife.com/forums/viewtopic.php?f=2&t=399&start=0 | 21:54 |
| kanzure | gah what's with you people and quitting | 21:54 |
| genehacker | cathedral building is making corrections as you go along | 21:54 |
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| nsh | the sheer level of lingo is impressive in itself | 21:54 |
| kanzure | nsh: do you read the NKS forums too? | 21:55 |
| * nsh reads things here and there :) | 21:55 | |
| genehacker | so what's golly? | 21:55 |
| nsh | golly is a life simulator | 21:55 |
| nsh | uses the hashlife algorithm, for speediness | 21:55 |
| kanzure | hi Roy78 .. you missed our longevity stem cell therapy stuff | 21:55 |
| kanzure | golly is a cellular automata simulator | 21:55 |
| genehacker | ok | 21:55 |
| nsh | http://golly.sourceforge.net/ | 21:55 |
| genehacker | so is that thing on that mans wall a time lapse? | 21:55 |
| nsh | no idea | 21:56 |
| kanzure | on the wall is a printout of the 200,000th iteration | 21:56 |
| genehacker | oh neat | 21:56 |
| kanzure | but it's more interesting to watch it animated | 21:56 |
| kanzure | because you see islands of order and stuff break off and go away.. | 21:56 |
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| kanzure | i just like watching it and making up stories :P | 21:56 |
| kanzure | "oh and now there's a colony" | 21:56 |
| genehacker | so I wonder if all this cellular automata stuff will actually be useful someday | 21:56 |
| lepton | *old and busted computer here at home | 21:56 |
| kanzure | "oh noes they might be dying" (and then thbey don't- providing a rail/structure for the snowball effect from the bigger former breakaway point to come over and takem the non) | 21:57 |
| genehacker | especially if we make real world cellular automata | 21:57 |
| kanzure | *take them on | 21:57 |
| kanzure | sure, people have done real world cellular automata | 21:57 |
| kanzure | paul rothemund did it on DNA once, i think | 21:57 |
| genehacker | oh snap | 21:57 |
| genehacker | paper link now | 21:57 |
| genehacker | I know that | 21:57 |
| kanzure | paul rothemund did your mom on dna, so whatever- not that impressive ;) he's a wizard | 21:57 |
| kanzure | lemme see | 21:58 |
| genehacker | someone made a glider gun in a cyclic chemical system | 21:58 |
| kanzure | hmm where did i put this paper | 21:58 |
| nsh | i'll be impressed when we get cellular automata processing information in, i don't know, convection cells on the ocean | 21:58 |
| genehacker | what I want to see is a cellular automata machine that can reconfigure itself into different speciallized computers | 21:59 |
| nsh | something massive and physical | 21:59 |
| nsh | i think you run into synch problems in real physical systems over a certain size | 21:59 |
| genehacker | yup | 21:59 |
| kanzure | genehacker: basically it was paul's sierpenski triangles on dna, that was cellular automata. | 21:59 |
| nsh | hard to have a 'clock' in the real world. damn you einstein... | 21:59 |
| nsh | i was reading a while back about emergent properties in asynchronous cellular automata | 22:00 |
| nsh | didn't get any clear conclusions though | 22:00 |
| kanzure | genehacker: http://www.dna.caltech.edu/Papers/SierpinskiDNA_PLoS2004.pdf | 22:00 |
| nsh | this looks interesting: http://citeseerx.ist.psu.edu/viewdoc/summary?doi=10.1.1.7.2073 | 22:01 |
| kanzure | Algorithmic Self-Assembly of DNA Sierpinski Triangles http://www.dna.caltech.edu/Papers/SierpinskiDNA_PLoS2004.pdf | 22:01 |
| kanzure | from http://www.dna.caltech.edu/DNAresearch_publications.html | 22:01 |
| genehacker | nsh quantum interactions are expected to be 10^35 times faster than the speed of light | 22:01 |
| * nsh doesn't know what that means | 22:01 | |
| kanzure | fake | 22:01 |
| genehacker | but only downside is you can only transfer information out of a quantum system at the speed of light... | 22:01 |
| kanzure | figures | 22:02 |
| nsh | i don't know if you can process anything using quantum interactions | 22:02 |
| nsh | i need to study quantum information theory | 22:02 |
| kanzure | nsh: what about dwave? | 22:02 |
| kanzure | the cfompany | 22:02 |
| kanzure | apparently i have significant investments in them O_o | 22:02 |
| kanzure | and the CEO is speaking at h+ summit | 22:02 |
| * nsh waits and sees | 22:02 | |
| kanzure | if you were quantum you would do your seeing first, and then your waiting | 22:02 |
| * nsh smiles | 22:02 | |
| genehacker | I have misgivings about putting a lot of money into dwave at this point in time | 22:03 |
| kanzure | i didn't choose to do it :P | 22:03 |
| kanzure | the old man did it before he died | 22:03 |
| nsh | i'm sure something will come of it | 22:04 |
| nsh | just perhaps not what's expected | 22:04 |
| nsh | science is the courtship of serendipity | 22:05 |
| kanzure | apparently the ceo (what's his name?) used their computing platform to solve the scheduling optimization problem for h+ summit | 22:05 |
| nsh | heh | 22:05 |
| genehacker | hopefully a quantum internet and not quantum cryto cracking | 22:05 |
| nsh | oh, i didn't get around to reading Post-quantum cryptography | 22:05 |
| nsh | or whatever it's called | 22:05 |
| nsh | http://www.springer.com/mathematics/numbers/book/978-3-540-88701-0 | 22:06 |
| nsh | have it knocking about somewhere | 22:06 |
| kanzure | do you need access to that springer link | 22:06 |
| nsh | nah, i got it already from somewhere | 22:07 |
| nsh | thanks though | 22:07 |
| nsh | "There's plenty of room at the bottom..." | 22:08 |
| kanzure | a lot of the stuff on the http://www.youtube.com/user/kanzure#grid/user/0EB93E6E02E5CF17 playlist looks like it'd fit right in with koyaanisqatsi | 22:09 |
| * kanzure is watching http://www.youtube.com/user/kanzure#p/c/0EB93E6E02E5CF17/37/wbDwUWGvwq4 now | 22:09 | |
| nsh | hmm | 22:10 |
| * nsh goes for a think | 22:14 | |
| InkBlob | kanzure, are you successful and famous or something? you're on youtube. | 22:14 |
| InkBlob | how much cash do you rake in ? | 22:14 |
| kanzure | i have some funding for crazy antics going on in here | 22:14 |
| genehacker | none from youtube though | 22:15 |
| kanzure | but i can't fund recurring payments like dojo membership fees ;-) or rent ;-) | 22:15 |
| genehacker | your facebook drug running should pay for that | 22:15 |
| InkBlob | so you have funding = sum of money, but that is not recurring monthly? | 22:15 |
| kanzure | the lump sum of money was a one time funding gig for me | 22:16 |
| kanzure | so once it's gone, it's gone | 22:16 |
| kanzure | and recurring payments are a really really quick way to use it up | 22:16 |
| kanzure | also i'm hesitant to spend money most of the time.. i's wierd | 22:16 |
| kanzure | i was kinda assuming i'd be broke eforever | 22:16 |
| kanzure | *weird | 22:16 |
| kanzure | *forever | 22:17 |
| kanzure | (i'm ssh'ing into another computer, and my home connection is slow, so that's why all these typos are happening) | 22:17 |
| Yocttar | So, any1 here can guide me to the next step after extracting my DNA? I wish to extract some algea DNA, manipulate it and let it reproduce eventually, so... Any1? :) | 22:18 |
| kanzure | algae reproduce on their own- why would you want to extract DNA from algae for reproduction? | 22:18 |
| genehacker | are you trying to do PCR? | 22:19 |
| Yocttar | __manipulate___ | 22:19 |
| Yocttar | ok, PCR then... how ? :) | 22:19 |
| genehacker | manipulate how? | 22:19 |
| kanzure | ah, for manipulation | 22:19 |
| InkBlob | i am conducting a project with my friends that is medical related. If it becomes successful, we can safely gain at least 15,000 per client (it will become service based.) We are looking for a person who is technically apt enough to understand what we are making (a mechanical device) and has enough expertise in biology. | 22:19 |
| kanzure | i guess there's all sorts of methods | 22:19 |
| Yocttar | lets say input some florecent gene or something | 22:19 |
| kanzure | oh, well then you should consider a plasmid method | 22:19 |
| kanzure | InkBlob: what is your project in particular? | 22:20 |
| genehacker | then you don't take the DNA out you put it in | 22:20 |
| InkBlob | The only thing we are worried about is that we are not licensed practioners of medicine. The particular license we need is probably somewhere along the lines of chiropractice. | 22:20 |
| genehacker | I have mechanical skills but I am just an undergrad | 22:20 |
| InkBlob | kanzure, I will speak to you in private after I gather the materials to show you what we are on to. | 22:21 |
| InkBlob | Is that ok with you? | 22:21 |
| kanzure | yes | 22:21 |
| kanzure | are you ok on a phone? | 22:21 |
| InkBlob | Please give me few days. | 22:21 |
| genehacker | is this something that goes inside people? | 22:21 |
| kanzure | speaking of which, someone recommended - anonymously- that we start having weekly conference calls | 22:21 |
| InkBlob | Maybe. I prefer logged chat for later reference. | 22:21 |
| kanzure | for the diybio, hackerspaces, open manufacturing, transhumanism cross-over community | 22:21 |
| InkBlob | genehacker: No. It's for aesthetic purposes, and is non-surgical based. | 22:22 |
| InkBlob | hence why it's more plausible. | 22:22 |
| kanzure | would anyone be interested in joining the teleconferences over skype? | 22:22 |
| kanzure | for some reason, people prefer voice chat for weekly meetings | 22:22 |
| kanzure | and don't take scheduled irc chats seriously (wtf) | 22:22 |
| genehacker | sounds interesting I would like to know more | 22:23 |
| kanzure | in particular i'd like to get you asshats back on the journal club reading schedule | 22:23 |
| kanzure | slackers | 22:23 |
| kanzure | all of you, just slackers. gah | 22:23 |
| genehacker | what schedule? | 22:23 |
| kanzure | weren't we doing a daily/weekly journal club thing? | 22:24 |
| genehacker | not that I know of | 22:24 |
| kanzure | hmm | 22:24 |
| Yocttar | Any guide for plasmid manipulation? | 22:24 |
| kanzure | tons :) | 22:24 |
| Yocttar | well , a simple one? :D | 22:25 |
| kanzure | http://protocol-online.org/ has a ridiculous load of links | 22:25 |
| Yocttar | I theoreticlly know the procedure | 22:25 |
| Yocttar | never in a lab | 22:25 |
| Yocttar | I can build w\e tools necessary | 22:26 |
| kanzure | the equipment for plasmid stuff is usually like a refrigerator, a freezer, petri dishes, an incubator, and more importantly the chemicals | 22:27 |
| kanzure | like for making the organism competent- the most basic version is a calcium chloride protocol | 22:28 |
| kanzure | but there are also methods involving gene guns, electrical techniques, natural competence (i.e. the organism might naturally take up DNA), and so on | 22:28 |
| Yocttar | Well, if that is the most basic method, I should look into it.. | 22:28 |
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| kanzure | it depends on your target organism, mind you | 22:29 |
| kanzure | often you have to tweak a protocol for plasmid transfection for a related organism if you're working with something bizarre/non-standard | 22:29 |
| Yocttar | for algea | 22:29 |
| kanzure | yeah algae transfection isn't exactly bio 101, but i don't recall how complicated it is | 22:29 |
| kanzure | most people do transfection with ecoli for starters, or something they can smear on a petri dish | 22:30 |
| Yocttar | ok then | 22:30 |
| kanzure | i'm not saying algae is impossible, just that it's not a typical first genetic engineering project | 22:30 |
| Yocttar | ecoli | 22:30 |
| genehacker | GFP algae? is someone trying to pull a prank on a school by dumping GFP algae into a fountain that happens to be on campus? | 22:30 |
| genehacker | because that sounds like a great idea | 22:30 |
| Yocttar | well | 22:30 |
| Yocttar | actually | 22:30 |
| Yocttar | my starting idea was | 22:30 |
| Yocttar | to put a glowing algea | 22:30 |
| Yocttar | in my fountain | 22:30 |
| Yocttar | wouldnt that be cool? :D | 22:30 |
| genehacker | there's already glowing algae out there | 22:30 |
| genehacker | or glowing bacteria | 22:31 |
| genehacker | can be obtained from REALLY fresh fish | 22:31 |
| kanzure | gfp only fluoresces in the presence of blue light, mind you | 22:31 |
| kanzure | mind you mind you mind you | 22:31 |
| kanzure | i need to stop saying that | 22:31 |
| genehacker | there's this one fountain I know of that uses blue lights | 22:32 |
| genehacker | yes the type that would make algae fluorecse | 22:32 |
| genehacker | anyway making stuff glow in the dark is hard | 22:33 |
| Yocttar | "can be obtained from REALLY fresh fish" , | 22:33 |
| genehacker | yup | 22:33 |
| Yocttar | what do you mean? | 22:33 |
| Yocttar | Lets say I buy a fish | 22:34 |
| Yocttar | ! | 22:34 |
| kanzure | oh no not the samon sperm thing again | 22:34 |
| kanzure | :( | 22:34 |
| Yocttar | a fresh 1 | 22:34 |
| Yocttar | O_o | 22:34 |
| kanzure | *salmon | 22:34 |
| genehacker | http://www.sciencemadness.org/talk/viewthread.php?tid=1485 | 22:34 |
| genehacker | nope | 22:34 |
| Yocttar | why would any fish have a glowing algea on him | 22:34 |
| genehacker | that's how we'd make synthetic DNA monomers though or some form of LED | 22:35 |
| genehacker | because it happens to live in the ocean | 22:35 |
| genehacker | it's bacteria actually, happens to grow on dead fish | 22:35 |
| genehacker | every seen bioluminescent stuff in the see when you agitate seawater? | 22:36 |
| genehacker | that's mostly that bacteria | 22:36 |
| Yocttar | nope I never saw that ;( | 22:38 |
| genehacker | go to an ocean sometime | 22:38 |
| genehacker | and get a bucket of water and agitate it | 22:38 |
| Yocttar | My fountain has sweet water tough ;( | 22:39 |
| Yocttar | that will work anywhere in the world you say? | 22:40 |
| genehacker | it depends | 22:40 |
| genehacker | sometimes | 22:40 |
| Yocttar | I dont think so =P | 22:40 |
| Yocttar | those bacteria must've taken over the world then | 22:40 |
| genehacker | you don't live in the US do you? | 22:41 |
| genehacker | they are fairly ubiquitous | 22:41 |
| genehacker | such that this can occur: http://weirdnewsfiles.com/wp-content/weirdnewsuploads/milky_sea.jpg | 22:42 |
| genehacker | that's a patch of sea where they all lit up | 22:42 |
| genehacker | yup they are present in all marine environments | 22:43 |
| kanzure | ooh http://www.programmableweb.com/apis/directory | 22:47 |
| Yocttar | Im from Israel | 22:47 |
| genehacker | excellent you have a nice patch of ocean which you can experiment on | 22:48 |
| Yocttar | ;D | 22:48 |
| genehacker | fresh fish should definately be nearby | 22:48 |
| genehacker | I live far too inland to extract luminous bacteria from fish | 22:48 |
| kanzure | good night robot army masquerading as human commandos | 22:49 |
| Yocttar | night ;) | 22:58 |
| Yocttar | (morning here) :) | 22:58 |
| genehacker | there is no such thing as night on the internet! | 22:58 |
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| Yocttar | Bah, so easy to find dna extraction process, nothing on insertion :< | 23:22 |
| Yocttar | any helpful forums\boards for extreme lab beginners like me? :p | 23:42 |
| genehacker | it's very specific to what you are working with | 23:42 |
| genehacker | if you really want experience take a class on it | 23:43 |
| genehacker | you could also read kanzure's paper repository | 23:43 |
| genehacker | but taking a class will get you more experience | 23:43 |
| genehacker | you could just go to the diybio google group | 23:45 |
| -!- marainein [~marainein@220.253-202-18.VIC.netspace.net.au] has joined #hplusroadmap | 23:52 | |
| Utopiah | Spiders at the Nanoscale: Molecules that Behave Like Robots http://cumc.columbia.edu/news/press_releases/DNArobot.html | 23:58 |
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