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| kanzure | why do you keep changing names on us | 11:45 |
|---|---|---|
| LukasDimoveo | because for some reason "Lukas" changes to Guest450645 | 11:45 |
| LukasDimoveo | or something like that | 11:46 |
| kanzure | because it's registered | 11:46 |
| kanzure | you should register a nickname too | 11:46 |
| LukasDimoveo | how do I do this? | 11:47 |
| kanzure | /query NickServ register | 11:47 |
| kanzure | but make sure you set your nickname to what you really want, first | 11:47 |
| LukasDimoveo | alright | 11:47 |
| LukasDimoveo | Anyway | 11:47 |
| LukasDimoveo | I want to do a simple bacterial transformation with bacteria commonly found in yogurt | 11:48 |
| kanzure | which bacteria in particular? | 11:48 |
| kanzure | lactobacillus xyz? | 11:49 |
| LukasDimoveo | yup | 11:49 |
| kanzure | once you know the actual name you can check for protocols on protocol-online.net | 11:50 |
| cluckj | sounds like a delicious experiment | 11:50 |
| LukasDimoveo | thank you | 11:50 |
| LukasDimoveo | I am targeting the "low hanging fruit" of human genetic engineering - engineering gut flora is the way to go until I can get some more gene therapy experience | 11:52 |
| kanzure | have you ever done a bacteria transformation project beforE? | 11:52 |
| kanzure | i'd suggest starting with the typical ecoli project.. | 11:52 |
| LukasDimoveo | I have done e.coli so many times I am sick of it | 11:53 |
| kanzure | it's a weekend project if anything | 11:53 |
| kanzure | ok | 11:53 |
| LukasDimoveo | (thinks about the 9-5 summer job - shutters) | 11:53 |
| LukasDimoveo | anyway, thanks for the link | 11:54 |
| kanzure | blah i should go pick out a dna sequencer for that guy today | 11:54 |
| LukasDimoveo | which guy, where? | 11:55 |
| kanzure | local guy | 11:59 |
| kanzure | i'm trying to figure out which model i want to buy | 12:00 |
| kanzure | so i thought i'd get the same model (abi prism 310) as the socal-diybio group | 12:00 |
| kanzure | but nothing is showing up on ebay at the moment, plus they have had.. problems with it | 12:00 |
| LukasDimoveo | Good to know | 12:08 |
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| cluckj | kanzure do you still have that transcript of the FBI dude at the open science summit? | 12:33 |
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| kanzure | cluckj: http://diyhpl.us/wiki/transcripts/open-science-summit-2010/ed-you/ | 12:58 |
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| kanzure | hm that's weird | 13:04 |
| kanzure | jrayhawk: if you go to http://diyhpl.us/wiki/recentchanges/ | 13:04 |
| kanzure | and then to the 2010-07-24 edits to 'cadfaq', | 13:04 |
| kanzure | it sends you to https://secure.diyhpl.us/repos/diyhpluswiki/ikiwiki.cgi?page=cadfaq&do=goto | 13:04 |
| kanzure | which requires a login | 13:04 |
| kanzure | er, well, the 'diff' is on /cgit so it's accessible | 13:06 |
| kanzure | that link is just a poorly formed redirect | 13:06 |
| cluckj | kanzure thanks | 13:10 |
| cluckj | I'm writing a paper about it | 13:10 |
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| kanzure | cluckj: i've added some stuff to http://diyhpl.us/wiki/transcripts/ | 13:34 |
| cluckj | nice | 13:43 |
| cluckj | are you going to the diy bio continental congress? | 13:43 |
| kanzure | no | 13:46 |
| kanzure | i'm taking a break from conferences :p | 13:47 |
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| -!- Topic for ##hplusroadmap: http://gnusha.org/skdb/ http://groups.google.com/group/diybio http://bit.ly/diybionews http://gitduino.com/ http://gadaprize.org/ | logs: http://gnusha.org/logs/ | 15:26 | |
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| -!- Topic for ##hplusroadmap: http://gnusha.org/skdb/ http://groups.google.com/group/diybio http://bit.ly/diybionews http://gitduino.com/ http://gadaprize.org/ | logs: http://gnusha.org/logs/ | 16:42 | |
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| kanzure | what's up with the ping timeouts | 17:06 |
| kanzure | haha, "biopunk book review" | 17:18 |
| kanzure | http://groups.google.com/group/bebobio/msg/887f88ea67e7e92d | 17:18 |
| kanzure | "Some of the | 17:18 |
| kanzure | stories have just enough information to pique one's interest, but not enough | 17:18 |
| kanzure | information to satisfy it." | 17:18 |
| kanzure | "For instance, I would have liked to have heard | 17:18 |
| kanzure | more about Bryan Bishop, whose prolific posts on the DIYbio message boards | 17:18 |
| kanzure | lead me to believe he is the epitome of a biopunk. " | 17:18 |
| kanzure | haha | 17:18 |
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| kanzure | hi epitron | 17:38 |
| kanzure | ok i posted my "which dna sequencer should i buy?" question | 17:41 |
| kanzure | http://groups.google.com/group/diybio/browse_thread/thread/e72c9bb618f0deb1 | 17:41 |
| kanzure | neat.. "Highly Efficient miRNA-Mediated Reprogramming of Mouse and Human Somatic Cells to Pluripotency" | 17:51 |
| kanzure | http://www.cell.com/cell-stem-cell/abstract/S1934-5909(11)00111-1 | 17:51 |
| kanzure | what "This miRNA-based reprogramming approach is two orders of magnitude more efficient than standard Oct4/Sox2/Klf4/Myc-mediated methods." | 17:52 |
| kanzure | well i guess that lives up to the title "Highly Efficient" | 17:52 |
| kanzure | paper: http://diyhpl.us/~bryan/papers2/stem-cells/Highly%20Efficient%20miRNA-Mediated%20Reprogramming%20of%20Mouse%20and%20Human%20Somatic%20Cells%20to%20Pluripotency.pdf | 17:57 |
| kanzure | ack wait | 17:57 |
| kanzure | it gave me the totally wrong paper? | 17:57 |
| kanzure | because pdx doesn't have a subscription. righto. i'll try another box then | 18:19 |
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| ybit | hey guys! favor to ask... :) | 20:17 |
| ybit | i need papers... | 20:17 |
| ybit | http://iopscience.iop.org/0953-4075/31/16/009 | 20:17 |
| ybit | http://iopscience.iop.org/0953-4075/33/12/309 | 20:17 |
| ybit | http://iopscience.iop.org/0953-4075/33/2/310 | 20:17 |
| ybit | and more... | 20:17 |
| ybit | http://dx.doi.org/10.1016/j.radphyschem.2003.12.024 | 20:21 |
| ybit | http://prl.aps.org/abstract/PRL/v83/i5/p939_1 | 20:21 |
| ybit | and that's all | 20:21 |
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| kanzure | ybit: also send the email to getarticles | 21:51 |
| ybit | okay | 21:55 |
| ybit | and it'd be interesting to know which papers are linked to the most in arxiv :) | 21:56 |
| ybit | thanks for the reminder | 21:57 |
| kanzure | so apparently todd, joe and matias each got $40k from the chilean government and are moving down there | 22:51 |
| jrayhawk | whaaat | 22:53 |
| kanzure | heck if i know. | 22:55 |
| kanzure | oh, not you | 22:55 |
| kanzure | the other joe http://www.youtube.com/watch?v=kmTNtSs1UQY | 22:56 |
| cluckj | lol what | 23:07 |
| cluckj | ahahahahahahahahahaha | 23:07 |
| cluckj | why do you have that | 23:07 |
| cluckj | also what are they doing in chile? lava amp stuff? | 23:11 |
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| JayDugger | Good morning, everyone. | 23:52 |
| --- Log closed Sun May 01 00:00:07 2011 | ||
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