| --- Log opened Thu Feb 09 00:00:02 2012 | ||
| -!- yashgaroth [~yashgarot@cpe-24-94-5-223.san.res.rr.com] has quit [] | 00:13 | |
| -!- _sol_ [~Sol@c-174-57-58-11.hsd1.pa.comcast.net] has quit [Ping timeout: 252 seconds] | 00:17 | |
| delinquentme | i think steve vai is going down hill | 00:55 |
|---|---|---|
| delinquentme | i take it back! | 00:56 |
| -!- delinquentme [~asdfasdf@c-24-3-85-154.hsd1.pa.comcast.net] has quit [Ping timeout: 276 seconds] | 01:43 | |
| -!- uniqanomaly [~ua@dynamic-78-9-73-39.ssp.dialog.net.pl] has joined ##hplusroadmap | 04:06 | |
| -!- _sol_ [Sol@c-174-57-58-11.hsd1.pa.comcast.net] has joined ##hplusroadmap | 06:06 | |
| -!- strages [~qwebirc@dev.throwthemind.com] has joined ##hplusroadmap | 06:13 | |
| @kanzure | Katherine Lugo, et al., Remote switching of cellular activity and cell signaling using light in conjunction with quantum dots, Biomedical Optics Express, 2012; [DOI:10.1364/BOE.3.000447] | 06:27 |
| @kanzure | http://www.opticsinfobase.org/boe/abstract.cfm?uri=boe-3-3-447 | 06:27 |
| -!- srangewarp [~strangewa@c-76-25-200-47.hsd1.co.comcast.net] has joined ##hplusroadmap | 06:29 | |
| @kanzure | "Remote switching of cellular activity by optical QD excitation is demonstrated by integrating QDs with cells: CdTe QD films with prostate cancer (LnCap) cells, and CdSe QD films and probes with cortical neurons. Changes in membrane potential and ionic currents are recorded by using the patch-clamp method. Upon excitation, the ion channels in the cell membrane were activated, resulting in hyperpolarization or depolarization of the cell." | 06:29 |
| @kanzure | CdTe QD films? how is that remote | 06:29 |
| -!- strangewarp [~strangewa@c-76-25-200-47.hsd1.co.comcast.net] has quit [Read error: Connection reset by peer] | 06:30 | |
| -!- srangewarp is now known as strangewarp | 06:30 | |
| @kanzure | Nanthia Suthana, et al., Memory Enhancement and Deep-Brain Stimulation of the Entorhinal Area, New England Journal of Medicine, 2012; 366 (6): 502 [DOI:10.1056/NEJMoa1107212] | 06:31 |
| @kanzure | http://www.nejm.org/doi/full/10.1056/NEJMoa1107212 | 06:31 |
| -!- JayDugger [~duggerj@pool-173-74-78-36.dllstx.fios.verizon.net] has joined ##hplusroadmap | 06:46 | |
| JayDugger | Good morning, everyone. | 06:48 |
| -!- klafka [~textual@c-71-204-150-80.hsd1.ca.comcast.net] has quit [Quit: Computer has gone to sleep.] | 07:18 | |
| -!- strages is now known as strages_work | 07:26 | |
| @kanzure | gah 'warfarin' is an awful drug name | 07:30 |
| -!- eudoxia [~eudoxia@r186-52-158-143.dialup.adsl.anteldata.net.uy] has joined ##hplusroadmap | 07:35 | |
| -!- Molybdenum [Molybdenum@173-30-249-153.client.mchsi.com] has joined ##hplusroadmap | 07:49 | |
| -!- eudoxia [~eudoxia@r186-52-158-143.dialup.adsl.anteldata.net.uy] has quit [Quit: Leaving] | 07:51 | |
| -!- jmil [~jmil@SEASNet-148-05.seas.upenn.edu] has joined ##hplusroadmap | 08:04 | |
| -!- Lucas_ [81317815@gateway/web/freenode/ip.129.49.120.21] has joined ##hplusroadmap | 08:35 | |
| Lucas_ | http://transhumani.com/topic29.html?sid=11b6e5018737b382a18f345e2445761e | 08:35 |
| @kanzure | what an awful url | 08:39 |
| @kanzure | what is it? | 08:39 |
| strages_work | diy electroporation | 08:40 |
| Lucas_ | diy electroporation | 08:41 |
| @kanzure | out of curiosity, why are you linking to that over the other diy electroporation posts out there | 08:42 |
| Lucas_ | I had no idea that DIY electroporation even existed. At least in the way that we intend to use it | 08:44 |
| @kanzure | -_- of course it does, look up the posts on diybio | 08:44 |
| Lucas_ | thank you | 08:46 |
| Lucas_ | another thing that you may be interested in: http://gizmodo.com/5882725/the-miraculous-nasa-breakthrough-that-could-save-millions-of-lives | 08:47 |
| @kanzure | nope, not interested in news sites | 08:47 |
| @kanzure | what is it? | 08:50 |
| @kanzure | ermererqekrjqioe | 08:50 |
| @kanzure | i meant to send: | 08:50 |
| @kanzure | just saw your skype message. | 08:50 |
| -!- augur [~augur@208.58.5.87] has quit [Remote host closed the connection] | 08:58 | |
| -!- jmil [~jmil@SEASNet-148-05.seas.upenn.edu] has quit [Remote host closed the connection] | 09:00 | |
| -!- augur [~augur@129.2.129.33] has joined ##hplusroadmap | 09:32 | |
| @kanzure | what is "application/x-Research-Info-Systems" | 09:33 |
| @kanzure | bleh | 09:34 |
| @kanzure | http://en.wikipedia.org/wiki/RIS_(file_format) | 09:34 |
| -!- Earl_ [~Earl@adsl-75-22-23-163.dsl.chcgil.sbcglobal.net] has joined ##hplusroadmap | 09:36 | |
| -!- Earl_ is now known as Guest11431 | 09:36 | |
| @kanzure | hi Guest11431 | 09:37 |
| -!- jmil [~jmil@SEASNet-148-05.seas.upenn.edu] has joined ##hplusroadmap | 09:38 | |
| -!- delinquentme [~asdfasdf@c-24-3-85-154.hsd1.pa.comcast.net] has joined ##hplusroadmap | 09:39 | |
| @kanzure | hi jmil, delinquentme | 09:40 |
| delinquentme | hoy! | 09:40 |
| jmil | o/ kanzure | 09:40 |
| jmil | o/ delinquentme | 09:41 |
| delinquentme | how many gits are there? | 09:41 |
| delinquentme | jmil, \/\/\o/\/\/ | 09:41 |
| jmil | lol | 09:41 |
| @kanzure | gits? | 09:42 |
| Mokbortolan_ | british word for "jerk" | 09:44 |
| delinquentme | Ghost in the shell :D | 09:53 |
| delinquentme | like my FAV | 09:53 |
| delinquentme | 2nd | 09:53 |
| delinquentme | no 3rd fav anime | 09:54 |
| Molybdenum | Depends on if you're talking about the actual comic or animations. | 09:55 |
| Mokbortolan_ | I didn't like the dialogue in the movie | 09:57 |
| delinquentme | do we know if 23 and me actively collaborates with google? | 09:57 |
| delinquentme | Molybdenum, howdy :D | 09:57 |
| Molybdenum | The original GitS movie was garbage, compared to the book. | 09:57 |
| delinquentme | havn't read the manga | 09:57 |
| delinquentme | but i was a fan of the movie | 09:58 |
| Molybdenum | heylo delinquentme | 09:58 |
| delinquentme | the hacker sentience scene | 09:58 |
| Molybdenum | You should read the manga. | 09:58 |
| delinquentme | LUV | 09:58 |
| delinquentme | you didn't like that DNA as the maintainer of humans discourse ? | 09:58 |
| Molybdenum | The comic is much, much more involved as far as the plot line and tech/science aspects of the GitS world in the comic. | 09:59 |
| Molybdenum | ugh, reduntant sentence. | 09:59 |
| Mokbortolan_ | I just liked the tank fight | 10:01 |
| Molybdenum | the tachikoma's are awesome | 10:02 |
| delinquentme | Oh and the dude with the extension of chargraffs DNA grammar wrote me back | 10:03 |
| delinquentme | seemed confident in the math.... | 10:03 |
| delinquentme | im a little confused as to why this isn't bigger than it is | 10:03 |
| @kanzure | delinquentme: 23andme doesn't collaborate with google as far as i know | 10:09 |
| delinquentme | not at all? | 10:11 |
| delinquentme | even though brin is married to one of the board members | 10:11 |
| -!- Lucas_ [81317815@gateway/web/freenode/ip.129.49.120.21] has quit [Ping timeout: 245 seconds] | 10:21 | |
| @kanzure | delinquentme: yes, to my knowledge there's no public program in place | 10:28 |
| -!- yashgaroth [~yaaic@66-87-0-189.pools.spcsdns.net] has joined ##hplusroadmap | 10:38 | |
| yashgaroth | kanzure, i should stress that the electroporator in that thread earlier is much different than the diybio posts | 10:39 |
| yashgaroth | as i'm targeting muscle tissue rather than bacteria, the parameters are much different | 10:40 |
| @kanzure | alright | 10:40 |
| yashgaroth | just fyi :V | 10:41 |
| @kanzure | yashgaroth: i'm wondering about how i'd create more demand to buy dna synthesizers | 10:41 |
| -!- nmz787 [43f2b117@gateway/web/freenode/ip.67.242.177.23] has joined ##hplusroadmap | 10:41 | |
| yashgaroth | tell the terrorists they can make smallpox with it, they have some cash | 10:41 |
| @kanzure | hi nmz787 | 10:41 |
| nmz787 | hola | 10:41 |
| @kanzure | maybe you could tell the hippies they can experiment with rational protein design for garbage/oil bacteria | 10:42 |
| @kanzure | but i gotta be honest, that's a boring application out of the possibilities | 10:42 |
| nmz787 | nootropics could be expressed in bacteria or yeast at home, and secreted... yogurt is a great example media for this | 10:42 |
| nmz787 | hippies grow yogurt | 10:43 |
| @kanzure | custom beers maybe | 10:43 |
| nmz787 | so if they could easily at home reprogram it | 10:43 |
| nmz787 | bam, market | 10:43 |
| strangewarp | I would fucking love some nootropic yogurt, flavored with jam | 10:43 |
| nmz787 | see | 10:43 |
| nmz787 | a new customer | 10:43 |
| nmz787 | step right up | 10:43 |
| @kanzure | but that's more of an argument for a custom yogurt company.. | 10:43 |
| Molybdenum | Mmm, burnt plastic flavor :| | 10:43 |
| strangewarp | very strong jam, ten | 10:44 |
| Molybdenum | piracetam powder still ranks as one of the foulest tasting things I've ever eaten. | 10:44 |
| strangewarp | then | 10:44 |
| yashgaroth | erm you're not gonna biosynthesize racetams, they're synthetic | 10:45 |
| nmz787 | with some genetic algorithms you could | 10:46 |
| yashgaroth | what | 10:46 |
| Molybdenum | ...you have my attention now. | 10:47 |
| @kanzure | he's suggesting a long and drawn out directed evolution process | 10:47 |
| @kanzure | that would eventually make a molecule that looks like your target | 10:47 |
| nmz787 | with inputs to the algorithm being yes or no from an on-chip TLC or something (validator), and the output being randomized DNA sequence for your target enzymes | 10:47 |
| nmz787 | yeah | 10:48 |
| @kanzure | metaboli network -> various synthetic outputs -> sequencing -> figure out which sequence caused that, throw that data into your GA | 10:48 |
| yashgaroth | that's far beyond anything possible now, much less DIY | 10:48 |
| @kanzure | not quite what i was expecting. | 10:48 |
| nmz787 | so if you could synth and sequence DNA on chip, you could then go on and say to express that DNA in bacteria, and yeah do directed evolution | 10:48 |
| @kanzure | yashgaroth: it's definitely a long process | 10:48 |
| nmz787 | ok well thats my version then | 10:49 |
| nmz787 | the hippy version would have a working pathway database | 10:49 |
| nmz787 | so you just download, then synth | 10:49 |
| nmz787 | and it electroprates your host on chip | 10:49 |
| nmz787 | or that could even be separate | 10:49 |
| @kanzure | a sequencer is an easy sell.. "see what weird creatures are all around you" "and potentially find new/useful genes somehow" | 10:49 |
| nmz787 | its been shown that nasally administered GFP DNA in mice made their liver grow | 10:50 |
| nmz787 | glow* | 10:50 |
| @kanzure | what about something like.. drosophila farming | 10:51 |
| nmz787 | umm | 10:51 |
| @kanzure | hrm, no | 10:51 |
| roksprok | what would you do with the drosophila? | 10:51 |
| yashgaroth | the technical term is 'ranching' | 10:51 |
| roksprok | eat them? | 10:52 |
| @kanzure | nope not drosophila, let's say "random microbes" instead | 10:52 |
| @kanzure | like from your water supply | 10:52 |
| @kanzure | and just do random electroporation/transformation. i don't know, that doesn't sound that fantastic to me | 10:52 |
| nmz787 | and seq? | 10:52 |
| @kanzure | right.. i guess | 10:52 |
| @kanzure | that would require a full genome or to splice in genes | 10:52 |
| nmz787 | @home sequencer is an easy sell i think | 10:52 |
| nmz787 | tell what your kids infection is | 10:52 |
| nmz787 | etc | 10:53 |
| @kanzure | maybe focusing on protein production in 'some culture' is better | 10:53 |
| nmz787 | foreseeable to even start faxing that data to your doctor, and let them tell you whether you need to get to the hospital, etc | 10:53 |
| nmz787 | yogurt man | 10:54 |
| nmz787 | milk media is found everywhere | 10:54 |
| @kanzure | i think protein production/purification is a separate target, besides just normal cell culturing / transformation | 10:54 |
| @kanzure | both require new dna | 10:54 |
| @kanzure | 'make stuff that you put into your body' might not be an acceptable approach | 10:55 |
| nmz787 | there's nothing wrong with keeping some toys for ourselves, we could offer a 'lead time' on the fancier ones | 10:55 |
| nmz787 | no its not | 10:55 |
| nmz787 | well | 10:56 |
| @kanzure | but it's the coolest. | 10:56 |
| nmz787 | it might be OK | 10:56 |
| nmz787 | yogurt and said organisms are GRAS | 10:56 |
| @kanzure | there's all sorts of things made possible, but i don't see that being obvious to potential customers | 10:57 |
| @kanzure | like, oh you're a polymer scientist.. well guess what can help you make interesting polymers | 10:57 |
| @kanzure | the educational side is pretty easy to sell | 10:57 |
| nmz787 | you wouldnt sell to the polymer guy, you'd sell to his biochem lab partner | 10:58 |
| @kanzure | labs don't buy dna synthesizers, but maybe this could change | 10:58 |
| nmz787 | oh? | 10:58 |
| @kanzure | if they are going to write it into the grant budget, they might as well just write in the costs for ordering something online | 10:58 |
| nmz787 | i assumed they did, since my school has one | 10:58 |
| @kanzure | i guess you could argue that high-throughput, sequence-specific dna generation opens up new possibilities in the lab | 10:59 |
| @kanzure | your school has *one* | 10:59 |
| @kanzure | and like 200 bio labs | 10:59 |
| nmz787 | not that many labs! | 10:59 |
| @kanzure | hmm | 10:59 |
| nmz787 | maybe 60-100 | 10:59 |
| nmz787 | courses | 11:00 |
| nmz787 | physical rooms prob more like 40-50 | 11:00 |
| roksprok | kanzure: could you make a case that it would be a ton quicker? | 11:00 |
| roksprok | instead of sending something out | 11:01 |
| roksprok | and waiting a few days to get it back | 11:01 |
| @kanzure | the design i'm thinking of could do multiple simultaneous different oligos | 11:01 |
| yashgaroth | i keep telling you, DHS keeps tabs on all registered dna synthesizers | 11:01 |
| roksprok | you could have it by the time you got back from lunch | 11:01 |
| @kanzure | not quite | 11:01 |
| @kanzure | (re: timing) | 11:01 |
| @kanzure | i believe the dhs thing though | 11:01 |
| @kanzure | the timing is more like, maybe it will take a few days | 11:01 |
| @kanzure | but it won't cost you $5,000 for that single molecule | 11:02 |
| roksprok | i still think that would be pretty compelling | 11:02 |
| @kanzure | well, what would you make? | 11:02 |
| @kanzure | you're not going to just buy a $5k synthesizer for fun | 11:02 |
| roksprok | i was still thinking of labs | 11:03 |
| roksprok | that use dna synthesis a bit | 11:03 |
| roksprok | but are limited by the fact that they have to go through a whole process of ordering, waiting, paying a decent chunk of change | 11:04 |
| roksprok | so don't do highly parallel things | 11:04 |
| roksprok | they could now move from 'make sure you know exactly what you need and absolutely need it" to "try a bunch of shit out" | 11:05 |
| roksprok | it seems like it would open up a bunch of low hanging fruit | 11:06 |
| yashgaroth | library generation for panning is a major user of dna synthesis | 11:06 |
| @kanzure | there's already dna library generation techniques, though you don't usually control the entire sequence (you just choose site-specific areas to mutate) | 11:06 |
| @kanzure | (and the mutations aren't under your control, in most of those methods) | 11:06 |
| @kanzure | panning? | 11:06 |
| yashgaroth | well, site-specific regions of maybe 20 residues | 11:07 |
| yashgaroth | yes, panning i'm not gonna describe cuz i'm on my phone keyboard | 11:07 |
| @kanzure | the common example is if you try out a million different versions of a polymerase, you might find one that is a million times better than the others, etc. | 11:07 |
| yashgaroth | well pol is pretty optimized, but a lot of biosynthesis pathways prob. have room for improvement | 11:08 |
| @kanzure | good point | 11:08 |
| @kanzure | so with 3d printers, the business model is "buy or make your open source 3d printer, then start a business around it and rent out time, or make custom parts for people" | 11:09 |
| @kanzure | "custom organisms" aren't quite going to be made possible with just a synthesizer (or even a synthesizer that can do some limited forms of transformation/incubation) | 11:09 |
| yashgaroth | it's like people who want to improve rubisco, not gonna happen, but many commercial pathways are still wild-type | 11:10 |
| strages_work | kanzure: or print and sell printers for others | 11:10 |
| yashgaroth | "custom" organisms as in adding a few genes is easy, just not a whole synthetic genome | 11:11 |
| @kanzure | well, i've always had this ludicrous idea that people would make custom dinoflagellates or weirdly-shaped bacteria | 11:11 |
| @kanzure | and then sell these as designer pets :P | 11:11 |
| yashgaroth | ehh the market for transgenic mammals that stay as neonates | 11:12 |
| yashgaroth | much bigger | 11:12 |
| @kanzure | then get a giant wall-mount lcd screen with a microscope hooked up to the screen, and it's a wall-mounted picture frame showing your strange microbial world | 11:13 |
| yashgaroth | you know you want a baby panda that stays that way | 11:13 |
| @kanzure | stay as neonates? | 11:13 |
| yashgaroth | perma-puppies | 11:13 |
| roksprok | what about replacing the bacteria in your mouth, with ones that don't reek | 11:13 |
| roksprok | as in, selling a kit to do it at home | 11:13 |
| roksprok | with bacteria you discovered and isolated with the help of the dna synthesizer | 11:13 |
| @kanzure | eh | 11:13 |
| @kanzure | why would the synthesizer help with that? | 11:14 |
| @kanzure | that's a very expensive kit.. | 11:14 |
| strages_work | altering the bacteria in your gut to allow for better digestion of things we don't usually digest properly | 11:14 |
| roksprok | wouldn't the synthesizer help in the development of the bacteria? | 11:14 |
| yashgaroth | gut is more near-term, esp. since e.coli is the most studied organisms ever | 11:14 |
| * delinquentme inserts your momma joke here | 11:15 | |
| delinquentme | sorry innapprope | 11:15 |
| delinquentme | <3 | 11:15 |
| @kanzure | yes except i don't think that's sellable on the market | 11:15 |
| @kanzure | Mr. VC: so you want people to buy your product.. so they can perform medical experiments? | 11:15 |
| @kanzure | designer microbes would go over better :P | 11:15 |
| delinquentme | well its marketable in the way that marinated cardboard is chinese chicken | 11:15 |
| roksprok | as in customizing it to out compete other mouth flora | 11:16 |
| roksprok | would it be possible to use the synthesizer to change algaes or plants? | 11:17 |
| roksprok | insert a gene or two? | 11:17 |
| @kanzure | sure.. | 11:17 |
| roksprok | maybe other hobbyist groups then | 11:18 |
| roksprok | like garderners or aquarium people | 11:18 |
| roksprok | some would have a lot of disposable income | 11:18 |
| yashgaroth | glowing plants for your aquarium or lawn | 11:18 |
| roksprok | and a few already spend shittons of money going on orchid hunting expeditions or something | 11:19 |
| @kanzure | yashgaroth: do you know how transformation works with seeds? | 11:19 |
| @kanzure | i'm familiar with agrobacterium as a method of transfection to a target plant | 11:19 |
| @kanzure | but i haven't looked at seed modification or if that's a thing | 11:19 |
| roksprok | or 'adapt this pretty plant to grow in your climate' then sell it to other gardeners | 11:19 |
| roksprok | that would at least be a bit of a business | 11:20 |
| roksprok | and hobbyists are used to spending tons of money on stuff other people see as a waste of time | 11:20 |
| @kanzure | yup, but to a large extent they don't currently do this (except maybe by crossing plants on their own) | 11:20 |
| roksprok | so i think their economics are a bit different than the usual consumers | 11:20 |
| yashgaroth | you don't need seed modding, you can grow a plant from a transformed cell | 11:21 |
| @kanzure | and there's barely any companies doing "designer microbes" except on a scale where they don't care about the high cost of dna synthesis mail ordering | 11:21 |
| @kanzure | sooo anything i throw together, would have to also help people along on that process | 11:21 |
| @kanzure | or something. not sure. | 11:21 |
| roksprok | are any companies doing designer microbes as pets/art/ not useful stuff yet? | 11:22 |
| @kanzure | not to my knowledge | 11:23 |
| yashgaroth | that guy who made a gfp rabbit for an art project, but not commercially | 11:23 |
| @kanzure | gardners don't exactly have biology labs, so i'd have to throw together a kit for that too | 11:23 |
| yashgaroth | oh, microbes, no | 11:23 |
| @kanzure | biofuel microbe design companies seem to exist, but it's sorta hush-hush because the oil companies don't want each other to know which strands they have been building | 11:24 |
| roksprok | could an igem like competition start for independent diybio people? | 11:25 |
| roksprok | or if more labs popped up you could get each one to buy a synthesizer or two | 11:26 |
| @kanzure | a competition might work.. | 11:27 |
| roksprok | if the costs came down maybe custom microbes could be a general part of industrial chemical research | 11:31 |
| roksprok | like, lets find something that works better than teflon or kevlar or whatever | 11:31 |
| roksprok | instead of just places where huge money is involved like energy | 11:32 |
| roksprok | and aren't there a couple medical products made with microbes? | 11:33 |
| yashgaroth | not many medical, but a lot of industrial enzymes | 11:37 |
| @kanzure | i'm not sure how many chemical plants are buying custom enzymes | 11:37 |
| yashgaroth | almost all biopharma prodxn is done in eukaryotes | 11:38 |
| yashgaroth | they'd buy if it works better | 11:38 |
| @kanzure | true, but the development timeline in pharma is decades not months | 11:38 |
| @kanzure | decades because of regulations etc | 11:38 |
| yashgaroth | yes, but not industrial enzymes, long as it's not going in your body | 11:39 |
| yashgaroth | pharma products are waaay out of our league to begin with | 11:40 |
| @kanzure | i'm just trying to imagine a scenario where there's a demand for 20,000 microbe designers | 11:41 |
| @kanzure | or 100,000 people with a dna synthesizer | 11:41 |
| @kanzure | *or enzyme designers | 11:41 |
| @kanzure | biofuels, drugs, bioplastics, biosensors, agriculture | 11:43 |
| yashgaroth | i can't imagine there's 20,000 unique projects to begin with, much less 100k | 11:43 |
| @kanzure | there's certainly more than 20,000 currently active biology projects in the US | 11:44 |
| @kanzure | antibodies. | 11:45 |
| @kanzure | you could scaremonger something about making new antibodies | 11:45 |
| @kanzure | (as long as the antibodies don't kill you) | 11:45 |
| @kanzure | "hey look, i'm fucking immune to mustard gas!" | 11:46 |
| @kanzure | dunno if that would work | 11:46 |
| yashgaroth | it's a bit too small, but a musard gas sequestering protein could work | 11:46 |
| @kanzure | btw if you don't think 20k, can you run me through what a good estimate would look like to you? | 11:47 |
| @kanzure | esp. of currently active research projects or biotech projects | 11:47 |
| yashgaroth | i mean, it's closer to 20k than 200, but that's all biotech, not just dna-synthesis-requiring projects | 11:49 |
| @kanzure | so you could say, great, buy this synthesizer if you want, make up some enzyme to help in some chemical factory process, | 11:51 |
| @kanzure | then start a chemical factory.. yeah um.. | 11:51 |
| yashgaroth | you can "plug in" a new enzyme to existing factory relatively easily, esp. with bacteria | 11:52 |
| @kanzure | oh dope i'm a dork | 11:53 |
| yashgaroth | ...if you have access to said factory, that is | 11:53 |
| @kanzure | there's already a lot of economic incentives related to carbon credits | 11:53 |
| @kanzure | and carbon offsetting. | 11:53 |
| @kanzure | es low. I highly recommend the Omnivore's Dilemma. It's generally trucked by the millions of tons to fatten up cows and generating tons of carbon waste. This is the form of carbon sequestering that is causing lots of problems in America and lots of other places. | 11:59 |
| @kanzure | bleh | 11:59 |
| @kanzure | i meant | 11:59 |
| @kanzure | http://diyhpl.us/wiki/transcripts/hplus-summit-2010/d1s1-3-andrew-hessel | 11:59 |
| @kanzure | so anyway.. i could see that as an argument for why there's lots of non-academic people that would buy synthesizers | 12:00 |
| -!- eudoxia [~eudoxia@r186-52-158-143.dialup.adsl.anteldata.net.uy] has joined ##hplusroadmap | 12:02 | |
| yashgaroth | heh "you don't have to leave facebook to do this" | 12:03 |
| -!- durth__ [96f3d7f7@gateway/web/freenode/ip.150.243.215.247] has joined ##hplusroadmap | 12:03 | |
| @kanzure | hi durth__ | 12:04 |
| @kanzure | yashgaroth: yeah :/ | 12:04 |
| durth__ | Hey | 12:05 |
| Mokbortolan_ | I for one welcome the grey goo. | 12:05 |
| durth__ | What goo? | 12:05 |
| eudoxia | I played a game yesterday where you were a gray goo particulate that grows and expands and consumes the entire e | 12:06 |
| eudoxia | universe | 12:06 |
| yashgaroth | like, left click to consume matter? | 12:08 |
| eudoxia | yeah | 12:08 |
| eudoxia | well, actually you just move it with the mouse, matter was consumed automatically, though I did spend the first ten minutes thinking I had to click | 12:08 |
| Mokbortolan_ | that sounds pretty boring | 12:14 |
| eudoxia | it was actually very addictive | 12:15 |
| Mokbortolan_ | in a minecraft sort of way? | 12:15 |
| Mokbortolan_ | or bejewelled | 12:15 |
| eudoxia | I guess it was the series of tiny little rewards that kept you going | 12:16 |
| -!- durth__ [96f3d7f7@gateway/web/freenode/ip.150.243.215.247] has quit [Ping timeout: 245 seconds] | 12:24 | |
| -!- eudoxia [~eudoxia@r186-52-158-143.dialup.adsl.anteldata.net.uy] has quit [Quit: Leaving] | 12:42 | |
| -!- devrandom [~devrandom@gateway/tor-sasl/niftyzero1] has joined ##hplusroadmap | 12:51 | |
| -!- yashgaroth [~yaaic@66-87-0-189.pools.spcsdns.net] has quit [] | 13:00 | |
| -!- Mokbortolan_1 [~Nate@c-71-59-241-82.hsd1.or.comcast.net] has joined ##hplusroadmap | 13:14 | |
| -!- Mokbortolan_ [~Nate@c-71-59-241-82.hsd1.or.comcast.net] has quit [Ping timeout: 245 seconds] | 13:16 | |
| @kanzure | huh, "a monkey's uncle" refers to darwin's work | 13:42 |
| @kanzure | for once a phrase makes sense? | 13:42 |
| Mokbortolan_1 | wouldn't the monkey be your uncle? | 13:44 |
| Mokbortolan_1 | you being the monkey's uncle would be devolution | 13:44 |
| Mokbortolan_1 | de-evolution? | 13:44 |
| Molybdenum | well, the disconnect with the term makes sense considering the kinds of people who get offended by Darwin's work. (And I'm hazarding the term started out as an insult) | 13:51 |
| roksprok | is there a webstore like sparkfun or dfrobot for the diybio community? | 13:56 |
| @kanzure | roksprok: not really | 13:56 |
| @kanzure | all of the chem suppliers are "too big to fail" | 13:56 |
| Mokbortolan_1 | "Well I'll be a Monkey's Uncle" = "I did not believe this was possible, but apparently it is." | 13:57 |
| @kanzure | Mokbortolan_1: yes but apparently it started to become a saying because of darwin's work | 13:58 |
| Mokbortolan_1 | but yeah, if they don't recognize its validity it's probably because they don't understand it, so the saying makes sense from that perspective :p | 13:58 |
| @kanzure | remember, for the longest time, people have always thought they were somehow superior or better than other lifeforms | 13:58 |
| @kanzure | so you'd be the "uncle" since you're better.. or something | 13:58 |
| Mokbortolan_1 | value judgements always get you in trouble | 13:59 |
| @kanzure | I AM BETTER THAN YOUR MOM | 13:59 |
| @kanzure | there, i said it.. | 13:59 |
| @kanzure | roksprok: equipment is usually expensive, so keeping stock is even more expensive | 14:04 |
| @kanzure | most people just hunt for pipettes on ebay | 14:04 |
| @kanzure | chemicals and reagants are a weird story | 14:04 |
| -!- Guest11431 [~Earl@adsl-75-22-23-163.dsl.chcgil.sbcglobal.net] has quit [Ping timeout: 252 seconds] | 14:08 | |
| @kanzure | god this is awful programming | 14:19 |
| @kanzure | http://research.microsoft.com/en-us/um/india/projects/biocoder/tutorial/section4.html | 14:19 |
| delinquentme | anyone know of publishing companies who will push your shit out for free? | 14:19 |
| roksprok | kanzure: the chemicals and reagants aren't controlled, are they? | 14:19 |
| @kanzure | measure_fluid(te, vol(20, UL), sterile_microfuge_tube2); | 14:19 |
| @kanzure | store_until(sterile_mirofuge_tube2, RT, ETHANOL_EVAP); | 14:19 |
| @kanzure | resuspend(sterile_microfuge_tube2); | 14:19 |
| @kanzure | roksprok: sometimes they are. the companies are definitely voluntarily controlling who they sell to, sometimes | 14:20 |
| roksprok | do you think there would be enough demand to kind of make a middle company? | 14:20 |
| @kanzure | based on my understanding of the economies of scale involved, no | 14:21 |
| roksprok | bummer | 14:21 |
| @kanzure | it's my understanding that sigma/aldrich, merck and otherso nly manufacture some of their chemicals | 14:21 |
| @kanzure | others are brought in from other companies on exclusive deals or whatever | 14:22 |
| @kanzure | if you want to build giant chemical factories that's cool, but i don't see this as being scalable | 14:22 |
| @kanzure | i mean, that's large investment stuff | 14:22 |
| roksprok | i was more thinking, order from merck or someone and repackage to sell to individuals or something | 14:23 |
| roksprok | so like, if you wanted to do a diy bacterial transformation you could get everything form one place | 14:23 |
| roksprok | *from | 14:23 |
| roksprok | and lower the barrier of entry | 14:24 |
| @kanzure | redistribution hrmm | 14:24 |
| roksprok | and maybe make kits | 14:24 |
| @kanzure | well that also depends on whether or not they will sell to you ;) | 14:24 |
| roksprok | well i was thinking if you set up a business, had proper zoning, etc. | 14:25 |
| roksprok | maybe present yourself as an education company? | 14:25 |
| roksprok | or a biotech startup? | 14:26 |
| roksprok | and were ordering in somewhat larger quantities | 14:26 |
| @kanzure | education company.. no thanks | 14:27 |
| @kanzure | well, kits are all the rage these days. so you'll pass the social acceptance test | 14:28 |
| roksprok | well it would more be to just explain it in a way that is less 'we are reselling your stuff to hobbyists we have no control over' and more 'we supply small colleges and startups' | 14:28 |
| roksprok | like i know there are a few kits out there already, but it seems there is a gulf between 'follow these exact steps' and 'do actual science' | 14:30 |
| roksprok | unlike say arduino, where sparkfun sells kits but also has a bunch of components and ideas for making your own stuff | 14:30 |
| roksprok | and a place to share them | 14:32 |
| roksprok | like its still a lot of work to get involved in diybio in a meaningful way | 14:32 |
| roksprok | it would be cool to smooth that process | 14:32 |
| @kanzure | have you see the process smoothed before, ever/ | 14:33 |
| @kanzure | like what do you mean- no testing required? | 14:33 |
| roksprok | well more like make it possible to go from 'wow synthetic biology is cool' to 'cool i made glow in the dark bacteria' to 'i'm actually being a bit innovative' | 14:35 |
| roksprok | like if i think robots are cool | 14:35 |
| roksprok | i can go get a kit to build a simple one | 14:35 |
| roksprok | see it follow a line or something | 14:35 |
| @kanzure | biology isn't like that, really | 14:36 |
| @kanzure | yes, you can follow steps in a kit to make bacteria glow | 14:36 |
| @kanzure | but oops, you touched your pipette tip to a surface and now you have to start over with your culture ;) | 14:36 |
| @kanzure | except you didn't realize it, and it didn't work "for some reason" even though you followed all steps | 14:37 |
| @kanzure | anyway, i think it's great if you want to make kits | 14:37 |
| roksprok | i guess its the "for some reason" thats the problem | 14:38 |
| roksprok | like in electronics if you short something out you can tell with a multimeter | 14:39 |
| @kanzure | you can test all the way through your experiment, but it's complicated | 14:39 |
| @kanzure | like there's no "dna contamination" multimeter | 14:39 |
| roksprok | yea and thats only one of the things that can go wrong | 14:40 |
| roksprok | i guess it would be easy to get discouraged | 14:40 |
| roksprok | moving away from that a bit, what about little test kits for fish? | 14:41 |
| roksprok | like, am i actually eating sea bass | 14:41 |
| roksprok | or did i just get ripped off | 14:41 |
| roksprok | would it be possible to make those cheaply? | 14:44 |
| roksprok | just a simple thing like a red or green led gets lit | 14:45 |
| @kanzure | i think macowell is making those at the moment | 14:45 |
| @kanzure | http://seqify.com/ | 14:45 |
| delinquentme | barcode fungi huh? | 14:47 |
| roksprok | but those you have to mail it off | 14:48 |
| roksprok | and they check it against a bunch of different species | 14:48 |
| roksprok | could you just use unamplified dna to do a single test? | 14:49 |
| @kanzure | so you want to do non-pcr-based testing? | 14:49 |
| roksprok | if possible | 14:49 |
| @kanzure | what mechanism will you use for dna barcoding then? | 14:50 |
| roksprok | could you have the desired dna strand | 14:50 |
| roksprok | and see if the sample binds to it? | 14:51 |
| @kanzure | http://scholar.google.com/scholar?q=hybridization+dna+barcoding | 14:52 |
| roksprok | ok scratch that then | 14:53 |
| roksprok | thanks | 14:53 |
| @kanzure | might be possible. you'll have to experiment and find out.. | 14:53 |
| roksprok | well it seems like segify's way is the best | 14:54 |
| roksprok | targeted at businesses | 14:54 |
| @kanzure | check out dna hybridization assays or something | 14:54 |
| roksprok | k | 14:55 |
| -!- Netsplit *.net <-> *.split quits: _sol_ | 15:09 | |
| -!- Netsplit over, joins: _sol_ | 15:09 | |
| @kanzure | hrm these guys do sequencer repair http://seqgen.com/ | 15:14 |
| delinquentme | kanzure, are you in #biology? | 15:16 |
| delinquentme | i asked a systems bio question and no one can derp on about it | 15:16 |
| @kanzure | are you asking about how to find out the kinetic equations for individual proteins/enzymes? | 15:17 |
| delinquentme | what im *REALLY after* is just machine learning w the internet as my datasource | 15:18 |
| @kanzure | what does that even mean | 15:18 |
| delinquentme | so like i go hunt down open journals | 15:18 |
| delinquentme | sort them by which has free associated data | 15:19 |
| delinquentme | gather multiples resources in this manner | 15:19 |
| delinquentme | XYZ | 15:19 |
| @kanzure | why.. | 15:19 |
| delinquentme | 4) profit! | 15:19 |
| @kanzure | are you trying to do whole cell simulation? | 15:19 |
| delinquentme | no im trying to figure out something to stick into this API so I can actually publish a paper | 15:19 |
| @kanzure | what api? | 15:20 |
| @kanzure | you can publish a paper about anything.. pick some crap from your blog, turn it into a paper | 15:20 |
| delinquentme | like a tutorial is cool but ive got a better chance at getting published with some sort of novel data | 15:20 |
| delinquentme | the one I was working on the the last few days | 15:20 |
| delinquentme | https://github.com/delinquentme/sapi | 15:20 |
| @kanzure | why do you want to publish a random paper? | 15:20 |
| @kanzure | wut.. why is this a dna api | 15:21 |
| @kanzure | why not just use fastaa files | 15:21 |
| delinquentme | so the paper is an example of how to do it properly | 15:26 |
| @kanzure | https://github.com/levskaya/BioJSON | 15:26 |
| @kanzure | delinquentme: i don't think that makes sense, there's already tons of tutorials on REST and wsdl and other crap | 15:27 |
| delinquentme | hmm that could be cool | 15:27 |
| delinquentme | make an API that you push up one file and it spits it back out as another file? | 15:27 |
| @kanzure | this is pretty nice: | 15:27 |
| @kanzure | https://github.com/levskaya/BioJSON/blob/master/genbank_parser.py | 15:27 |
| @kanzure | delinquentme: why | 15:27 |
| @kanzure | delinquentme: what are you doing?? | 15:27 |
| delinquentme | kanzure, I want to make a API that does something novel | 15:28 |
| delinquentme | in order to present it as a research paper | 15:28 |
| delinquentme | So end goal would be something like | 15:28 |
| delinquentme | " An example tutorial and presentation for XYZ " | 15:29 |
| delinquentme | " An example tutorial on restful API implementation and presentation for XYZ biological function" | 15:29 |
| @kanzure | restful sequence alignment would be nice | 15:31 |
| -!- jmil [~jmil@SEASNet-148-05.seas.upenn.edu] has quit [Quit: jmil] | 15:32 | |
| delinquentme | kanzure, do you know a "Daniel Wilkerson" ? | 15:37 |
| delinquentme | micheal rae connected me with him .. and hes kinda wigging out on me | 15:38 |
| roksprok | what does wigging out mean? | 15:41 |
| roksprok | is that a british thing? | 15:42 |
| delinquentme | getting angry like call caps typing | 15:44 |
| delinquentme | over asking him questions | 15:44 |
| -!- strages_shop [~strages@256.makerslocal.org] has joined ##hplusroadmap | 15:47 | |
| roksprok | delinquentme: i like that term i'd never heard it before thanks | 15:52 |
| @kanzure | caps or cops | 15:55 |
| @kanzure | i know a rouillie wilkerson who's into diybio | 15:55 |
| @kanzure | and a shawn wilkerson who's into transhumanism | 15:55 |
| Mokbortolan_1 | I wouldn't say that I'm into transhumanism | 16:01 |
| Mokbortolan_1 | I mean, I don't have anything against them | 16:01 |
| Mokbortolan_1 | if you wanna turn from a girl to a boy or vice versa, who am I to criticize? | 16:01 |
| -!- jmil [~jmil@c-68-81-252-40.hsd1.pa.comcast.net] has joined ##hplusroadmap | 16:02 | |
| Molybdenum | err. | 16:15 |
| Mokbortolan_1 | (it was a joke) | 16:17 |
| -!- augur [~augur@129.2.129.33] has quit [Remote host closed the connection] | 16:19 | |
| Stee| | job acquired | 16:38 |
| @kanzure | "For those of you who don't get the github messages, the C++ generated for AP235TC and AP242 n2813 now compiles. All we need now are exchange files to test with!" | 16:55 |
| @kanzure | "This leaves AP209 as the only schema that fedex_plus fails on, and AP210e2/e3 as the only schemas that gcc won't compile." | 16:55 |
| -!- augur [~augur@208.58.5.87] has joined ##hplusroadmap | 17:11 | |
| delinquentme | whats that Steel? | 17:19 |
| delinquentme | Stee|, ^ | 17:19 |
| -!- augur [~augur@208.58.5.87] has quit [Remote host closed the connection] | 17:21 | |
| -!- augur [~augur@208.58.5.87] has joined ##hplusroadmap | 17:22 | |
| ybit | video recommendations for while i'm eating? | 17:28 |
| @kanzure | team fourstar | 17:29 |
| -!- eudoxia [~eudoxia@r190-135-30-213.dialup.adsl.anteldata.net.uy] has joined ##hplusroadmap | 17:31 | |
| ybit | heh :) | 17:34 |
| -!- sylph_mako [~mako@118-93-230-101.dsl.dyn.ihug.co.nz] has joined ##hplusroadmap | 17:42 | |
| JayDugger | Great Scapel Mistakes on the Home Surgery Channel. | 17:49 |
| -!- yashgaroth [~yashgarot@cpe-24-94-5-223.san.res.rr.com] has joined ##hplusroadmap | 18:10 | |
| delinquentme | so what do we get from studying grass | 18:18 |
| delinquentme | like is this the same thing as algae? that we want ot understand it to modify it to create chemicals? | 18:18 |
| delinquentme | or is this only going to net us better grass for football stadiums? | 18:19 |
| * delinquentme doesnt get it | 18:19 | |
| yashgaroth | you mean, general research on grass biology? | 18:19 |
| -!- augur [~augur@208.58.5.87] has quit [Remote host closed the connection] | 18:20 | |
| delinquentme | yeah | 18:29 |
| delinquentme | like im looking at a 1mil grant from the NIH for a group to study tomatoes ... when someone working on a highspeed computer network for genomic transfers .. got 200k ? | 18:30 |
| delinquentme | like how? why? | 18:30 |
| -!- Mokbortolan_ [~Nate@c-71-59-241-82.hsd1.or.comcast.net] has joined ##hplusroadmap | 18:31 | |
| JayDugger | Given the result, imagine the motivations of the funding agency's decision makers. | 18:31 |
| yashgaroth | because programming only requires stimulants and a terminal, while bio research has a lot of expensive consumables | 18:31 |
| JayDugger | And that's an even better answer. | 18:32 |
| yashgaroth | unless you mean the physical network for large data transfer, in which case I don't see why it's specific to genomes | 18:32 |
| delinquentme | yash its for some big research groups to make a highspeed network | 18:33 |
| -!- Mokbortolan_1 [~Nate@c-71-59-241-82.hsd1.or.comcast.net] has quit [Ping timeout: 272 seconds] | 18:33 | |
| delinquentme | so that they're not mailing hard drives | 18:33 |
| delinquentme | but JayDugger I just have no idea what could come out of indepth study of grass | 18:33 |
| JayDugger | Think about what might motivate people. | 18:34 |
| delinquentme | to research grass? | 18:34 |
| delinquentme | cattle feed... | 18:34 |
| delinquentme | maybe something with cloroplasts | 18:35 |
| JayDugger | No, you still think about grass. Why would people make that decision. | 18:35 |
| delinquentme | sun >> energy? | 18:35 |
| JayDugger | Banal and quotidian reasons matter more than the merits. | 18:35 |
| delinquentme | converting particular soil chemicals? | 18:35 |
| yashgaroth | do you have a link to the grant posting? | 18:35 |
| delinquentme | JayDugger, so you're saying its the NIH buddy system? | 18:36 |
| delinquentme | bc that was my first guess | 18:36 |
| yashgaroth | I'm sure suburbanites wouldn't mind if their lawn sequesters pollutants, if they're gonna mow it anyway | 18:36 |
| delinquentme | http://www.nsf.gov/awardsearch/showAward.do?AwardNumber=1125620 | 18:36 |
| JayDugger | I don't know. Cynical answers can prove wrong, but when they do it comes as a pleasant surprise. | 18:37 |
| yashgaroth | also rice is a grass | 18:37 |
| delinquentme | but regular old normal grass doesnt sequester pollutants... soooo why are we trying to fix this? | 18:37 |
| yashgaroth | because if we could make it do that, it'd be useful | 18:38 |
| yashgaroth | also that grant is about studying corn, which apparently is also a grass, TIL | 18:38 |
| delinquentme | ah | 18:39 |
| delinquentme | so the american corn growers association or some shit is involved ? | 18:39 |
| delinquentme | that i can see | 18:39 |
| yashgaroth | I think corn is the #1 crop in the US by acres planted, at least #2 | 18:39 |
| yashgaroth | oh yeah it's #1 by about >3x acres the #2, soy | 18:40 |
| yashgaroth | a 1% improvement in yield or nutrition from that would be huuuge | 18:41 |
| delinquentme | "internet 1 " | 18:47 |
| delinquentme | "internet 2 " !!! | 18:48 |
| delinquentme | see | 18:49 |
| delinquentme | THIS | 18:49 |
| delinquentme | http://www.nics.tennessee.edu/computing-resources/kraken | 18:49 |
| delinquentme | this i useful | 18:50 |
| -!- JayDugger [~duggerj@pool-173-74-78-36.dllstx.fios.verizon.net] has quit [Quit: Leaving.] | 18:54 | |
| -!- sylph_mako [~mako@118-93-230-101.dsl.dyn.ihug.co.nz] has quit [Ping timeout: 252 seconds] | 19:04 | |
| -!- Molybdenum [Molybdenum@173-30-249-153.client.mchsi.com] has quit [Quit: Leaving] | 19:07 | |
| -!- eudoxia [~eudoxia@r190-135-30-213.dialup.adsl.anteldata.net.uy] has quit [Quit: Leaving] | 19:16 | |
| -!- kvltist [~Kvltist@p5B33E8C0.dip.t-dialin.net] has joined ##hplusroadmap | 19:18 | |
| -!- industromatic [~chatzilla@cpe-70-113-67-117.austin.res.rr.com] has joined ##hplusroadmap | 19:19 | |
| industromatic | hey Bryan | 19:19 |
| industromatic | What do you think about Quinonez, Carlo | 19:20 |
| industromatic | Seems like a non-friend of open hardware. | 19:24 |
| industromatic | Gotta go. | 19:24 |
| -!- industromatic [~chatzilla@cpe-70-113-67-117.austin.res.rr.com] has quit [Quit: ChatZilla 0.9.88 [Iceweasel 6.0/20110827095134]] | 19:24 | |
| yashgaroth | caralo quinonez is a dick! oh he left | 19:26 |
| @kanzure | yashgaroth: why is he a dick? | 19:32 |
| yashgaroth | I have no idea who he is | 19:32 |
| @kanzure | yashgaroth: http://groups.google.com/group/diybio/t/11855037508b9774 | 19:32 |
| @kanzure | sciencedirect makes me angry. they set a new cookie each time you load a page, apparently | 19:33 |
| yashgaroth | so do you mostly use IDs from that ezproxy page? and if so do you have a currently working one | 19:35 |
| @kanzure | i ssh into a few servers that are inside good networks | 19:36 |
| @kanzure | let me see about a good one.. uhh i don't suppose an ezproxy login for ncbi.nih.gov would be ok | 19:37 |
| yashgaroth | hopefully a uni library with decent subscriptions | 19:39 |
| @kanzure | right.. i had one in mind | 19:44 |
| @kanzure | that's why it's taking me a few moments.. | 19:44 |
| yashgaroth | at least until Stee| shows up for me to annoy about articles | 19:44 |
| @kanzure | yashgaroth: bradfordcollege | 19:46 |
| @kanzure | ezproxy.free-webmaster-resourcsmies.org/201201/shibboleth-bradfordcollege-ac-uk-library-ezproxy-username-and-password-20120114/ | 19:46 |
| @kanzure | this isn't the one i was thinking of though :( | 19:46 |
| @kanzure | delinquentme: pm | 20:19 |
| -!- n_bentha [~lolicon@75.111.160.104] has joined ##hplusroadmap | 20:28 | |
| -!- strages_shop [~strages@256.makerslocal.org] has quit [Ping timeout: 265 seconds] | 20:36 | |
| -!- delinquentme [~asdfasdf@c-24-3-85-154.hsd1.pa.comcast.net] has quit [Quit: Leaving] | 20:41 | |
| -!- delinquentme [~asdfasdf@c-24-3-85-154.hsd1.pa.comcast.net] has joined ##hplusroadmap | 20:42 | |
| -!- augur [~augur@208.58.5.87] has joined ##hplusroadmap | 20:50 | |
| -!- nmz787 [43f2b117@gateway/web/freenode/ip.67.242.177.23] has quit [Quit: Page closed] | 20:54 | |
| delinquentme | http://www.iscb.org/index.php?option=com_content&view=article&id=342&lqm_uid=45 << wish they offered this for free =[ | 20:58 |
| @kanzure | well that looks like joomla | 20:59 |
| @kanzure | so just use a joomla exploit | 20:59 |
| @kanzure | oh it's a class. | 20:59 |
| delinquentme | dat cert | 21:06 |
| -!- jmil [~jmil@c-68-81-252-40.hsd1.pa.comcast.net] has quit [Quit: jmil] | 21:54 | |
| -!- devrandom [~devrandom@gateway/tor-sasl/niftyzero1] has quit [Quit: leaving] | 22:03 | |
| -!- Mokbortolan_1 [~Nate@c-71-59-241-82.hsd1.or.comcast.net] has joined ##hplusroadmap | 22:10 | |
| n_bentha | yashgaroth: it werks! | 22:13 |
| yashgaroth | congrats! | 22:13 |
| -!- Mokbortolan_ [~Nate@c-71-59-241-82.hsd1.or.comcast.net] has quit [Ping timeout: 244 seconds] | 22:13 | |
| yashgaroth | so, the reason you didn't need a functional gene is that you're doing gene repression with a the fragment in that plasmid? or what | 22:14 |
| n_bentha | yeah | 22:15 |
| yashgaroth | don't worry, I haven't guesssed which gene you're knocking down | 22:15 |
| yashgaroth | can you tell me what plant it's in, or will that give away too much? | 22:16 |
| @kanzure | don't say cannabis don't say cannabis | 22:17 |
| n_bentha | omg i wish i could do some work on cannabis | 22:17 |
| yashgaroth | man, putting the THC synthesis genes into hops would be so easy, they're almost the same species | 22:17 |
| yashgaroth | practically the same biosynthetic pathway already, it's only a few extra genes and bam | 22:18 |
| @kanzure | yashgaroth: funny story.. it's not much of a story actually | 22:18 |
| @kanzure | but the mol bio professors at ut were talking once and i overheard them, | 22:18 |
| @kanzure | apparently every other student talks about doing just that | 22:18 |
| yashgaroth | haha I'm not surprised | 22:19 |
| @kanzure | and they're cool with it, but after like 30 years one of them have followed through | 22:19 |
| @kanzure | *none of them | 22:19 |
| yashgaroth | well, the full pathway only got published a couple years ago | 22:19 |
| delinquentme | so i guess it makes sense ... but theres is serious complexity implications when inserting genes | 22:19 |
| -!- klafka [~textual@c-71-204-150-80.hsd1.ca.comcast.net] has joined ##hplusroadmap | 22:19 | |
| delinquentme | so the closer the organisms are the easier it is? | 22:19 |
| yashgaroth | generally | 22:19 |
| yashgaroth | there's no complexity implied when you can copy over the gene and its regulatory sequence straight over | 22:20 |
| delinquentme | r/trees would love to know how close those two things are | 22:20 |
| n_bentha | and omg who doesn't love hops? | 22:20 |
| delinquentme | how beer is totally acceptable | 22:20 |
| delinquentme | ^^^^^^^^^^^^ | 22:20 |
| n_bentha | weed-beer | 22:20 |
| delinquentme | what he said | 22:20 |
| klafka | hey | 22:20 |
| klafka | ? | 22:20 |
| klafka | what are you guys talking about | 22:20 |
| yashgaroth | >_> | 22:21 |
| @kanzure | delinquentme fucked up and added a cannabis biosynthesis gene network to his fuckin' gut | 22:21 |
| * delinquentme wants some methon throat spray | 22:21 | |
| @kanzure | now he's high all the time. | 22:21 |
| klafka | aah | 22:21 |
| yashgaroth | it's a positive feedback loop, the genes are activated by cheetos | 22:21 |
| delinquentme | http://www.youtube.com/watch?v=RsfPFsN_z2U | 22:21 |
| delinquentme | oh! so yeah that sequencing tech based off video compression .. thats the kinda thing you'd use to sequence the gut microbes huh? | 22:22 |
| yashgaroth | is this the one for finding minority species in a mixture and getting their genomes? then yes | 22:22 |
| n_bentha | i'd love to have some guy microbes w/ certain genes that i could kill easily | 22:24 |
| delinquentme | I got MY computational bioinformatics PHD at devry university for FI dollar! | 22:24 |
| n_bentha | or at least turn off their expression by eating something | 22:24 |
| delinquentme | n_bentha, i think that happens all the time | 22:24 |
| delinquentme | no. wait | 22:24 |
| yashgaroth | inducible suicide genes tend to get selected out pretty quick | 22:25 |
| delinquentme | the stuff you eat changes the expressions within genes within 24 hours | 22:25 |
| delinquentme | but im not sure if it said anything about killing off microbes | 22:25 |
| yashgaroth | unless you mean killing gene expression | 22:25 |
| n_bentha | well i want a system where i can throw some genes into bacteria, make a pie, sprinkle them on top, and then eat it, have the microbes produce my proteins of interest, and then die when i eat some spice, or something? | 22:26 |
| delinquentme | FDA-approved drug rapidly clears amyloid from the brain, reverses Alzheimer's symptoms in mice | 22:27 |
| delinquentme | http://medicalxpress.com/news/2012-02-fda-approved-drug-rapidly-amyloid-brain.html | 22:27 |
| yashgaroth | why not keep the bacteria around and just have the spice activate protein expression? | 22:27 |
| n_bentha | yes or that, yashgaroth. some form of control. | 22:28 |
| yashgaroth | it's always nice to have the option to kill them, true | 22:29 |
| n_bentha | what is this black magic, delinquentme? the work of witches i say! burn them all! | 22:29 |
| delinquentme | n_bentha, its much like that claimed "chemistry" whitchcraftery | 22:30 |
| delinquentme | they dont wear burkas so we better burn them to be safe | 22:30 |
| delinquentme | hail jeebus | 22:30 |
| delinquentme | oh! and i've been posting the fuck out of a passage in numbers which lays out the directions to curse a child concieved outside of wedlock | 22:31 |
| n_bentha | lolol | 22:31 |
| yashgaroth | pretty sure the bible already says to stone everyone to death for something or other | 22:32 |
| delinquentme | my primary anti-theistic argument? You'd think an omnicient being would have the capacity to create a bit more scalable teachings | 22:32 |
| delinquentme | then they say " he wants us to decipher right from left" | 22:32 |
| delinquentme | then why say anything at all jeebus? | 22:32 |
| n_bentha | "Megadoses produced a 50% reduction in plaque in the brains of those mice in 72 hours." --wikipedia on the drug Bexarotene | 22:35 |
| delinquentme | <3 | 22:35 |
| -!- delinquentme [~asdfasdf@c-24-3-85-154.hsd1.pa.comcast.net] has quit [Quit: Leaving] | 22:35 | |
| * n_bentha makes a run for it | 22:41 | |
| -!- n_bentha [~lolicon@75.111.160.104] has quit [Quit: n_bentha] | 22:41 | |
| -!- kanzure_ [~kanzure@131.252.130.248] has joined ##hplusroadmap | 22:48 | |
| -!- saurik_ [~saurik@carrier.saurik.com] has joined ##hplusroadmap | 22:48 | |
| -!- dsp__ [~tt@lebesgue.cowpig.ca] has joined ##hplusroadmap | 22:49 | |
| -!- kanzure [~kanzure@131.252.130.248] has quit [Write error: Connection reset by peer] | 22:50 | |
| -!- saurik [~saurik@carrier.saurik.com] has quit [Write error: Connection reset by peer] | 22:50 | |
| -!- dsp_ [~tt@lebesgue.cowpig.ca] has quit [Read error: Connection reset by peer] | 22:50 | |
| -!- nchaimov [~nchaimov@c-67-171-214-94.hsd1.or.comcast.net] has quit [Ping timeout: 244 seconds] | 22:54 | |
| -!- CIA-126 [~CIA@cia.atheme.org] has quit [Ping timeout: 244 seconds] | 22:56 | |
| -!- CIA-9 [~CIA@cia.atheme.org] has joined ##hplusroadmap | 22:56 | |
| -!- nchaimov [~nchaimov@c-67-171-214-94.hsd1.or.comcast.net] has joined ##hplusroadmap | 23:00 | |
| -!- Netsplit *.net <-> *.split quits: uniqanomaly, audy | 23:02 | |
| -!- Netsplit over, joins: audy | 23:07 | |
| -!- uniqanomaly [~ua@dynamic-78-9-73-39.ssp.dialog.net.pl] has joined ##hplusroadmap | 23:10 | |
| kanzure_ | argh freenode | 23:12 |
| -!- qnm [~qnm@2001:44b8:3110:f300:208:9bff:fec0:179a] has quit [Ping timeout: 416 seconds] | 23:15 | |
| -!- qnm [~qnm@2001:44b8:3110:f300:208:9bff:fec0:179a] has joined ##hplusroadmap | 23:17 | |
| -!- saurik [~saurik@carrier.saurik.com] has joined ##hplusroadmap | 23:23 | |
| -!- SolG [Sol@c-174-57-58-11.hsd1.pa.comcast.net] has joined ##hplusroadmap | 23:23 | |
| -!- saurik_ [~saurik@carrier.saurik.com] has quit [Write error: Connection reset by peer] | 23:24 | |
| -!- gnusha_ [~gnusha@131.252.130.248] has joined ##hplusroadmap | 23:32 | |
| -!- Topic for ##hplusroadmap: biohacking, nootropics, transhumanism, open hardware http://gnusha.org/logs/ http://bit.ly/diybionews2 http://gadaprize.org/ http://groups.google.com/group/openmanufacturing | 23:32 | |
| -!- Topic set by kanzure [~kanzure@131.252.130.248] [Sun Jan 8 13:52:13 2012] | 23:32 | |
| [Users ##hplusroadmap] | 23:32 | |
| [ AlonzoTG] [ epitron ] [ kanzure_ ] [ SolG ] | 23:32 | |
| [ archels ] [ fenn ] [ klafka ] [ Stee| ] | 23:32 | |
| [ audy ] [ ferrousw1eel ] [ kvltist ] [ strages_home] | 23:32 | |
| [ audy- ] [ foucist ] [ Mokbortolan_1] [ strages_work] | 23:32 | |
| [ augur ] [ gedankenstuecke] [ nchaimov ] [ strangewarp ] | 23:32 | |
| [ bkero ] [ gnusha ] [ nuba ] [ superkuh ] | 23:32 | |
| [ Charlie_] [ gnusha_ ] [ Omega ] [ uniqanomaly ] | 23:32 | |
| [ CIA-9 ] [ Guest427 ] [ pasky ] [ Urchin ] | 23:32 | |
| [ Coornail] [ Guest46680 ] [ qnm ] [ utopiah_ ] | 23:32 | |
| [ drazak ] [ Helleshin ] [ quannum ] [ yashgaroth ] | 23:32 | |
| [ dsp__ ] [ ivan` ] [ roksprok ] [ ybit ] | 23:32 | |
| [ elmom ] [ jrayhawk ] [ saurik ] [ ziyadb ] | 23:32 | |
| -!- Irssi: ##hplusroadmap: Total of 48 nicks [0 ops, 0 halfops, 0 voices, 48 normal] | 23:32 | |
| -!- Channel ##hplusroadmap created Thu Feb 25 23:40:30 2010 | 23:32 | |
| -!- Irssi: Join to ##hplusroadmap was synced in 4 secs | 23:32 | |
| -!- dsp_ [~tt@lebesgue.cowpig.ca] has joined ##hplusroadmap | 23:32 | |
| yashgaroth | I really don't get who would DOS freenode | 23:33 |
| -!- drazak [~ahdfadkfa@drazak.net] has quit [Ping timeout: 248 seconds] | 23:33 | |
| -!- Guest46680 [freenode@s83.seattle.pd.wastate.net] has quit [Ping timeout: 248 seconds] | 23:33 | |
| -!- dsp__ [~tt@lebesgue.cowpig.ca] has quit [Ping timeout: 248 seconds] | 23:33 | |
| -!- gnusha [~gnusha@131.252.130.248] has quit [Ping timeout: 248 seconds] | 23:33 | |
| -!- Charlie_ [~quassel@64.31.59.70] has quit [Ping timeout: 248 seconds] | 23:33 | |
| -!- Urchin [~urchin@unaffiliated/urchin] has quit [Ping timeout: 248 seconds] | 23:33 | |
| -!- drazak [~ahdfadkfa@drazak.net] has joined ##hplusroadmap | 23:33 | |
| -!- audy [~audy@heyaudy.com] has quit [Ping timeout: 245 seconds] | 23:34 | |
| -!- quannum [~qnm@2001:44b8:3110:f300:208:9bff:fec0:179a] has quit [Ping timeout: 245 seconds] | 23:34 | |
| -!- audy- is now known as audy | 23:34 | |
| -!- yashgaroth [~yashgarot@cpe-24-94-5-223.san.res.rr.com] has quit [] | 23:43 | |
| -!- klafka [~textual@c-71-204-150-80.hsd1.ca.comcast.net] has quit [Ping timeout: 471 seconds] | 23:49 | |
| -!- klafka [~textual@c-71-204-150-80.hsd1.ca.comcast.net] has joined ##hplusroadmap | 23:49 | |
| -!- pasky_ [pasky@nikam.ms.mff.cuni.cz] has joined ##hplusroadmap | 23:50 | |
| -!- pasky [pasky@nikam.ms.mff.cuni.cz] has quit [Ping timeout: 276 seconds] | 23:50 | |
| -!- ziyadb [u4806@gateway/web/irccloud.com/x-cgvoqysjxylxfepo] has quit [Max SendQ exceeded] | 23:50 | |
| -!- ziyadb [u4806@gateway/web/irccloud.com/x-vvyhnlhwgbsnwddh] has joined ##hplusroadmap | 23:50 | |
| -!- ziyadb [u4806@gateway/web/irccloud.com/x-vvyhnlhwgbsnwddh] has quit [Changing host] | 23:50 | |
| -!- ziyadb [u4806@unaffiliated/ziyadb] has joined ##hplusroadmap | 23:50 | |
| -!- ziyadb [u4806@unaffiliated/ziyadb] has quit [Changing host] | 23:50 | |
| -!- ziyadb [u4806@gateway/web/irccloud.com/x-vvyhnlhwgbsnwddh] has joined ##hplusroadmap | 23:50 | |
| -!- uniqanomaly [~ua@dynamic-78-9-73-39.ssp.dialog.net.pl] has quit [Ping timeout: 276 seconds] | 23:50 | |
| -!- uniqanomaly [~ua@dynamic-78-9-73-39.ssp.dialog.net.pl] has joined ##hplusroadmap | 23:51 | |
| -!- klafka [~textual@c-71-204-150-80.hsd1.ca.comcast.net] has quit [Ping timeout: 244 seconds] | 23:56 | |
| -!- Charlie_ [~quassel@64.31.59.70] has joined ##hplusroadmap | 23:57 | |
| -!- Guest427 [~quassel@64.31.59.70] has quit [Ping timeout: 240 seconds] | 23:58 | |
| -!- klafka [~textual@c-71-204-150-80.hsd1.ca.comcast.net] has joined ##hplusroadmap | 23:58 | |
| --- Log closed Fri Feb 10 00:00:03 2012 | ||
Generated by irclog2html.py 2.15.0.dev0 by Marius Gedminas - find it at mg.pov.lt!