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kanzure | http://www.digital-bio.com/micro/neon.htm "A novel, benchtop electroporation device that | 02:31 |
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kanzure | employs an microporation technology." | 02:31 |
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foucist | micropenis technology? gosh! | 03:15 |
kanzure | like a boss | 03:21 |
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delinquentme | I pick one door out of 10,000. The game host removes 9,998 other doors ... leaving two doors: My initial choice door and another. Im told the prize is behind one of these doors. I switch to the *OTHER* door because it is more probable that that door has the prize. the *WHY* is what im after.. and I THINK it is because unlike my initial door choice the other door does not have the initial associated probability of 1:10, | 06:46 |
delinquentme | 000 of being correct. | 06:46 |
delinquentme | if anyone is really good w stats :D | 06:46 |
ThomasEgi | that depends | 06:49 |
ThomasEgi | if the game host puts the price behind the door befor you pick yours. then chances are that the price is behind the door the computer picks. | 06:50 |
uniqanomaly | https://en.wikipedia.org/wiki/Monty_Hall_problem | 06:50 |
ThomasEgi | if the computer just picks a random door. and then puts the price behind one of the 2 remaining. it makes no difference which one you pick | 06:50 |
chris_99 | yay just picked up an Electrophoresis PSU for £26 :) | 06:52 |
delinquentme | ^^ | 07:38 |
kanzure | beep boop | 07:38 |
delinquentme | ThomasEgi, you're saying that if the prize door is set before you pick the initial door and it stays at that door then you should switch | 07:39 |
delinquentme | agreed. | 07:39 |
delinquentme | im just not sure if its because theres a prior probability | 07:39 |
delinquentme | i *THINK* thats why | 07:39 |
delinquentme | howdy kanzbot | 07:39 |
delinquentme | veridical paradox ??? | 07:40 |
kanzure | delinquentme: pm | 07:42 |
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kanzure | hmm | 10:16 |
kanzure | http://gen9bio.com/ | 10:16 |
kanzure | "A small number of Gen9’s current production tools generate sufficient DNA content to, in principal, replace the world’s current annual output of synthetic genes." | 10:16 |
kanzure | "based on next generation gene synthesis technologies." | 10:16 |
chris_99 | know what tech. they use? | 10:17 |
kanzure | probably ligation | 10:17 |
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delinquentme | kanzure, you mentioned getting the average length of all genes which exist in the online repositories right? | 10:35 |
kanzure | uh sure | 10:37 |
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delinquentme | yeah that it would be a comparison worth pursuing right? | 10:50 |
delinquentme | howd the pitch go? | 10:50 |
kanzure | went well | 10:58 |
kanzure | maradydd is telling me that IDT *builds* its own DNA synths | 10:59 |
kanzure | http://eu.idtdna.com/pages/home/about-us/what-we-do | 10:59 |
kanzure | "DT manufactures the oligo using chemical DNA synthesis machines designed and built in-house." | 10:59 |
kanzure | they found it was cheaper to just hire some dude to build synths | 10:59 |
kanzure | than to buy synths on the market | 10:59 |
delinquentme | with what equipment? | 11:00 |
kanzure | what? | 11:00 |
delinquentme | i like arguing too much | 11:02 |
delinquentme | how does that individual build the oligos | 11:03 |
kanzure | he makes the machines | 11:03 |
kanzure | not the oligos | 11:03 |
delinquentme | oh | 11:03 |
delinquentme | fuck | 11:03 |
delinquentme | haha | 11:03 |
delinquentme | whos this dude? | 11:03 |
kanzure | nobody in particular- i think it might be a team | 11:03 |
kanzure | probably just some engineer | 11:03 |
kanzure | nothing special.. just told to make synthesizers for the company | 11:03 |
delinquentme | PS i still dont have a good answer for a web server stack to power the backend of a networked open hardware system | 11:04 |
delinquentme | am i totally bonkers for saying " fuck it " and building it w rails? | 11:05 |
delinquentme | i see no reason not to | 11:05 |
kanzure | you are totally bonkers yes | 11:06 |
delinquentme | why :D | 11:06 |
delinquentme | is the question | 11:06 |
delinquentme | i mean in *this* instance | 11:06 |
delinquentme | i know why in general | 11:06 |
kanzure | why does it need to be a web server? | 11:07 |
delinquentme | bc i want it networked | 11:08 |
delinquentme | and thats my hammer | 11:08 |
delinquentme | im in startups trolling as well | 11:08 |
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delinquentme | so if the majority of the server processing im going to be doing is going to be protocols | 11:14 |
delinquentme | i definitely need something event based | 11:15 |
delinquentme | so EM is a big part of that | 11:15 |
delinquentme | nginx and thin are EM based I believe | 11:15 |
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yash-phone | fucking internet | 11:54 |
Mariu | kanzure, what do you think of Gregory Stock ? | 11:55 |
Utopiah_ | http://www.wired.com/epicenter/2012/03/opinion-wolfram-life-analytics/ | 11:56 |
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delinquentme | just finished chatting w a christian | 12:46 |
delinquentme | said hes probably the smartest person on earth | 12:47 |
delinquentme | #truestory | 12:47 |
ThomasEgi | godfags. allways good for some saturday evening entertainment | 12:48 |
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Mariu | delinquentme, what channel ? | 12:59 |
delinquentme | #ai | 12:59 |
delinquentme | dropped a few questions on him | 12:59 |
delinquentme | i closed the chat | 13:00 |
delinquentme | how do you bring up past convos? | 13:00 |
delinquentme | or the convo history? | 13:00 |
kanzure | do you lg? | 13:00 |
kanzure | *log | 13:00 |
Urchin | delinquentme: it would depend on your client | 13:00 |
Mariu | indeed | 13:06 |
Urchin | look up xchat's manual | 13:08 |
Urchin | or dig trough the options | 13:08 |
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foucist | delinquentme: i usually just go into the terminal, find the log file, and grep it | 13:14 |
foucist | or open in vim etc | 13:14 |
Urchin | I usually just use less | 13:16 |
kanzure | i use dd | 13:17 |
kanzure | screw all of you | 13:17 |
Urchin | wtf? | 13:18 |
kanzure | haha | 13:19 |
kanzure | Urchin: i am implying i use something unnecessarily ridiculous | 13:19 |
kanzure | and thus it is funny or humorous | 13:19 |
kanzure | also the stormstrooper shot is pretty rad: http://i.imgur.com/1r6bl.jpg | 13:19 |
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Mariu | that's so cool, thanks kanzure | 13:22 |
delinquentme | xchat is the client | 13:22 |
delinquentme | lol here we go | 13:22 |
delinquentme | <DanFrederiksen> intelligence is not monolithic but in terms of true value and overall combined capability I am as smart as they come. perhaps the smartest on the planet | 13:22 |
delinquentme | and *christian* | 13:23 |
delinquentme | ulululuz | 13:23 |
Mariu | lol | 13:23 |
kanzure | ah another person who believes in intelligence | 13:26 |
kanzure | well i guess that's what happens in #ai | 13:26 |
Urchin | ol | 13:26 |
Urchin | put it on fstdt | 13:26 |
Urchin | *lol | 13:26 |
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delinquentme | FSDT? | 13:27 |
Urchin | http://www.fstdt.com/ | 13:28 |
Urchin | ofcourse: http://www.fstdt.com/Top100.aspx | 13:29 |
delinquentme | LOL | 13:33 |
delinquentme | awesome | 13:33 |
kanzure | protein engineering tutorial http://openwetware.org/wiki/20.109%28S12%29:Start-up_protein_engineering_%28Day1%29 | 13:37 |
kanzure | "lyse, lyse baby" http://www.seringhaus.net/lyse.html | 13:38 |
kanzure | Yo, PhD – let’s mix it. | 13:38 |
kanzure | Alright stop, grab some yeast and a test tube, | 13:38 |
kanzure | Stick it in buffer that’s cold like an ice cube, | 13:38 |
kanzure | Nothin’ – gonna grow when you cool it, | 13:38 |
kanzure | Split the bug up, slap it on a GeneChip, | 13:38 |
kanzure | Will it bind the probe? Yo – I can’t guess | 13:38 |
kanzure | Turn off the lights, it’ll fluoresce | 13:38 |
kanzure | This lab, bitch, is dope delirium | 13:38 |
kanzure | Knockin’ out genes in some bacterium | 13:38 |
kanzure | Staph, Pseudomonas, E.coli | 13:39 |
kanzure | Layin’ the pain on a mutant fruit fly | 13:39 |
kanzure | Phage, suckers kill bacteria | 13:39 |
kanzure | Got RNA genomes so don’t let em near ya | 13:39 |
yashgaroth | fffffff | 13:39 |
kanzure | Love it or leave it, it ain’t DNA, | 13:39 |
kanzure | But does show up on a microarray, | 13:39 |
kanzure | If you got a genome, yo, fantastic, | 13:39 |
kanzure | Check SWISSPROT while you waitin’ for your BLAST hit | 13:39 |
kanzure | yashgaroth: YOU WILL ENJOY THIS | 13:39 |
kanzure | or else | 13:39 |
yashgaroth | my legs, they're...moving...to the beat! | 13:39 |
kanzure | Yo man – Let’s get outta here. Cells to the shaker. | 13:39 |
MetaMorph | kanzure, keep it... procreative... yo? | 13:40 |
delinquentme | oh man rhymed verse | 13:40 |
delinquentme | ach | 13:40 |
delinquentme | painful | 13:40 |
kanzure | hi MetaMorph | 13:41 |
kanzure | delinquentme: gotta know your rhymes | 13:41 |
* MetaMorph is. | 13:42 | |
delinquentme | kanzure, talk to me when you've lived through these times | 13:42 |
* delinquentme blows brains out | 13:42 | |
delinquentme | lol | 13:42 |
delinquentme | kanzure, what liquid volumes are microfluidics @ | 13:44 |
delinquentme | not pico liters right? | 13:44 |
kanzure | sometimes picoliter | 13:45 |
kanzure | sometimes femtoliters | 13:45 |
delinquentme | waaaooowww | 13:46 |
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delinquentme | well i guess size of the syringe would also help | 13:47 |
kanzure | huh? | 13:47 |
kanzure | http://diyhpl.us/~bryan/papers2/microfluidics/Droplet%20microfluidics.pdf | 13:47 |
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delinquentme | encoder resolution <> smallest vol dispensed reliably <> syringe size | 13:50 |
delinquentme | if i need high resolution.. just use smaller syringes | 13:50 |
kanzure | or you just pump liquid through at a certain pressure | 13:51 |
delinquentme | what pressures do they run at? | 13:52 |
delinquentme | im not going to need special syringes am i? | 13:52 |
delinquentme | nL pL fL yeah | 13:52 |
delinquentme | kk | 13:52 |
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kanzure | "Blue Heron Bio's GeneMaker® proprietary, novel and high throughput design and synthesis platform synthesizes genes and synthetic DNA sequences economically and with perfect accuracy." | 14:06 |
kanzure | "Jamie Heywood or people in his group - rogue scientists who were trying to come with a cure for ALS which Heywood’s brother had." | 14:07 |
kanzure | "Our first team, led by Shawn Mikula (working in the laboratory of Winfried Denk at the Max Planck Institute in Heidelberg), has developed a whole mouse brain chemical preservation and plastic embedding technique. A preliminary overview of the protocol used can be found at http://connectomes.org/ . " | 14:13 |
kanzure | "This innovative approach begins by transcardially perfusing the mouse with the fast-diffusing fixative acrolein (as opposed to the more traditional fixatives of paraformaldehyde and glutaraldehyde). The protocol then uses simple immersion of the entire mouse brain in solutions including osmium tetroxide over a two day period to achieve fixation of lipids throughout the brain." | 14:14 |
kanzure | "second team, 21st-Century Medicine led by the cryobiologist Greg Fahy, has developed a method of perfusing entire rabbits with very high concentrations of the cryoprotective agent M22 (a formula that they have developed for commercial use in organ cryopreservation for transplantation). " | 14:14 |
kanzure | "at the BPF have also made progress in obtaining access to the equipment and facilities needed for evaluating the prize. Last month, I personally visited Halcyon Molecular (a biotech startup company based in San Franscisco) which has graciously offered to let us use their electron microscope facilities to help in prize evaluation. " | 14:14 |
kanzure | pfft | 14:14 |
delinquentme | 3 5 10 30 60 | 14:20 |
delinquentme | visionary: im gonna make a simple AI which only knows plant-related terms and im going to sell it on the app store... and name it "words with fronds" | 14:21 |
kanzure | your visions don't sound that intense | 14:21 |
kanzure | have you tried drugs | 14:21 |
delinquentme | LOL | 14:24 |
delinquentme | but its plant material | 14:24 |
delinquentme | OMGZ #biology will comprehend my mastery | 14:24 |
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delinquentme | kanzure, happen to know whos the go-to syringe supplier out there/ | 14:28 |
kanzure | not off han | 14:32 |
kanzure | hand | 14:32 |
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delinquentme | heh. maths is awesome | 15:58 |
delinquentme | true life story... nL are hella frikkin small | 16:52 |
delinquentme | kanzure, what do people use as nL pumps currently? | 16:52 |
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roksprok | delinquentme: http://www.sigmaaldrich.com/labware/labware-products.html?TablePage=101225104 | 20:26 |
roksprok | nanoliter pumps | 20:26 |
roksprok | that you probably already know about | 20:26 |
roksprok | only 2000 dollars | 20:26 |
roksprok | and backordered | 20:26 |
delinquentme | :D | 20:27 |
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kanzure | delinquentme: they use expensive syringe pumps | 21:10 |
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ybit | idea: kickstarter for FLOSS development | 22:29 |
ybit | yay/nay? | 22:29 |
Steel2 | FLOSS? | 22:29 |
ybit | open source software | 22:29 |
Steel2 | what is the FL part? | 22:30 |
Steel2 | freeware license? | 22:30 |
ybit | free/libre | 22:30 |
ybit | Steel2: think linux | 22:31 |
ybit | kde | 22:31 |
ybit | gnome | 22:31 |
ybit | python | 22:31 |
ybit | haskell | 22:31 |
ybit | etc. | 22:31 |
kanzure | FLOSS is a pretty standard acronym | 22:31 |
kanzure | ybit: why not use kickstarter? | 22:32 |
ybit | i don't see any software projects on there | 22:32 |
kanzure | oh iirc it's against their terms | 22:32 |
ybit | i dunno if it's allowed | 22:32 |
kanzure | i guess that's an issue. | 22:32 |
ybit | huh | 22:32 |
kanzure | they don't want you to put your kickstarter project on kickstarter | 22:33 |
ybit | ah | 22:33 |
ybit | well here's the problem... | 22:33 |
ybit | kde e.v. has been in the talks with http://sfconservancy.org/ for a couple of years now about getting a NPO started in the states but SFC got back with them sometime last year to let them know that they just didn't have the capacity | 22:34 |
ybit | http://www.youtube.com/watch?v=9q8LTZSvpr8&feature=player_embedded | 22:34 |
ybit | i'm watching bradley kuhn from there give his presentation ^ | 22:34 |
kanzure | what about spi or something | 22:34 |
ybit | they fund individual developers | 22:35 |
kanzure | spi? | 22:35 |
ybit | what is that? | 22:35 |
kanzure | software in the public interest, inc. (a 501c3) | 22:35 |
ybit | probably because nobody had heard of them | 22:35 |
kanzure | http://www.spi-inc.org/ | 22:35 |
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ybit | i'll contact them | 22:36 |
kanzure | uh really? debian, drupal, yafaray, haskell, libreoffice, jenkins, fluxbox | 22:36 |
kanzure | stop me if you've heard of any of these :/ | 22:36 |
kanzure | oh and arch linux apparently. neat.. | 22:36 |
ybit | first time i've heard of haskell | 22:36 |
ybit | and wtf is debian | 22:36 |
kanzure | and postgresql | 22:36 |
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kanzure | you know, it's somewhat alarming the number of postgresql installations i've done work on but haven't written direct queries for | 22:38 |
kanzure | compared to mysql where the ratio is probably flipped | 22:38 |
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ybit | http://pyvideo.org/category/17/pycon-us-2012 :: pycon 2012 vids | 22:43 |
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joshcryer | <3 yafaray | 23:02 |
klafka | hi | 23:07 |
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--- Log closed Sun Mar 11 00:00:46 2012 |
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