2012-03-21.log

--- Log opened Wed Mar 21 00:00:00 2012
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archelshttp://www.amazon.com/Metabolic-Molecular-Bases-Inherited-Disease/dp/007913035601:16
archels$721 for a genetics textbook?01:16
archelsIt's pretty huge, but still...01:17
fennSteel3: something like that chemistry app exists, it's called oscar http://stark-fire-6273.herokuapp.com/chemoinformatics/new01:48
fenner, click browse01:49
fennmore about oscar http://blogs.ch.cam.ac.uk/pmr/2011/11/25/the-scandal-of-publisher-forbidden-textmining-the-vision-denied/01:51
joshcryernot sure if real http://www.humanbirdwings.net/02:17
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Reploparchels:  and extracting information from it would be far slower than from a database with the same content03:09
Replopand carrying an usb thumb drive is far easier than 4 huge vollumes totalling 6338  pages.03:10
ThomasEgibesides. you can also stuff entire wikipedia on thet usb-thumb drive, and a copy if LotR and you still have enough space for a few of you fav songs03:12
Replophttp://www.ommbid.com/  << same title.  but I doubt they propose a downloadable offline version.03:12
ThomasEgihow about a recursive wget on that?03:13
Replopwould only get abstracts.03:13
Replopfull texts are hidden behind paywalls03:13
* ThomasEgi hates paywalls :(03:14
Replopand they don't even display the price before asking for personal information03:14
Replopoh. I was wrong03:15
Replophttp://www.ommbid.com/OMMBID/a/ommbidrate03:15
Replop $295  for full access03:15
Replopfar cheaper than the paper version, then03:15
ThomasEgihm. if someone has access to it, running a small python script that makes your browser go over the individual pages found in the table of content, and saving it to disk wouldnt be too hard03:16
Replopjust make the script tweak the pages so they would be nicely browsable offline.03:17
Reploprelative links and such03:17
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ThomasEgionce you have the actual content. that's not much of a problem at all03:19
Replopyes03:19
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ThomasEgimatter of running a single replace() on each html page03:20
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Replopbut of course, uploading the result for the rest of the community would be highly illegal :/03:47
ThomasEgiat least you could carry it arround on your thumbdrive...04:04
ThomasEgilosing that drive, wouldbe... an ... unfortunate loss04:04
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UrchinI think we're going to lose peer review in a not too distant future04:35
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kanzureevidence that the finger-magnet community is more of an offshoot of BMEzine than transhumanism:06:32
kanzurehttp://www.iamdann.com/2012/03/21/my-magnet-implant-body-modification06:32
vrs"more than" why think in those terms?06:35
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kanzurehi ParahSailin_06:44
ParahSailin_hi06:44
ParahSailin_fucking carpal tunnel06:45
kanzurebeing 20, i am invincible and do not experience such problems06:46
ParahSailin_i normally dont either06:47
ParahSailin_but the gf is a biter06:47
ParahSailin_and i think she did some nerve damage06:47
thesnarkkanzure you're only 20? nice06:48
strangewarpjegus, I've got to work harder06:51
strangewarpI'm almost 25 and still putting together my first major thing :P06:52
thesnarkstrangewarp best to always work your hardest06:53
strangewarpI'm on the nootropics and they definitely help06:58
kanzurestrangewarp: rule 1 of ##hplusroadmap is, i'm gonna beat the snot outta ya06:59
kanzure25 is the new 5006:59
strangewarpgoddamnit07:00
kanzurei'm just being a jerk07:00
thesnarkshit, except that 25 really is the new 5007:01
thesnarkI have have 2 years, 2 months ;__;07:01
kanzure25? isn't that the time when most SV programmers retire?07:01
thesnarkhaha07:01
thesnarkseems like it07:02
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Coornail25?07:18
CoornailI have to work harder then07:18
CoornailI wan't to retire07:18
CoornailI had enough of this mess some call software development...07:19
thesnarkoh come on07:19
thesnarkyou get a sick pleasure from it07:19
thesnarkadmit it07:19
CoornailI kinda do...07:19
CoornailI would like to retire though07:20
thesnarkI think what you mean is that you'd like to focus only on stuff you find interesting07:20
kanzurethesnark: i think i broke protocol-online.org07:21
Coornailisn't it what "retiring" means?07:21
thesnarknice kanzure07:22
thesnarkthat looks so ripe for scraping07:22
thesnarkCoornail well, if that's your definition of retiring you can start whenever you want07:22
kanzurethesnark: wait, does it load?07:22
thesnarkyeah07:22
kanzurehahah07:23
thesnarkthey probably blocked you07:23
kanzurehe's blocking me07:23
kanzurewhat a cockblocker07:23
thesnarkthis is why we need our distributed scraping darknet07:23
thesnarkI could have shared the load ;)07:23
kanzurewelll07:23
kanzurei found this huge hole on the server i think07:23
kanzurewhere if you scrape a particular page,07:23
kanzureit sends out emails to 20,000 users07:23
thesnarkbahahaha07:23
kanzureand each email takes 30 seconds to generate07:23
thesnarkhahaha07:23
thesnarkthat is awesome07:23
Coornailsomebody there will really wish he/she could retire =)07:24
kanzurethesnark: http://diyhpl.us/~bryan/irc/protocol-online/protocol_scraper.py07:24
thesnarkkanzure nice yeah BeautifulSoup is good07:25
thesnarkyou should throttle your scraping though07:26
kanzureNEVER.07:26
kanzurethesnark: btw07:26
kanzurea distributed scraper throttler that works on top of the 'requests' library would be very easy07:26
kanzurelike a drop-in replacement07:26
kanzurei guess you would have to use some async library like twisted though07:26
katsmeow-afkthat's a *lot* of code for such a simple task07:26
kanzurekatsmeow-afk: i wanted to parse the data07:27
* thesnark must go to class07:28
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katsmeow-afkprolly enough data linked thru there to keep everyone's dsl in here busy for a month07:29
thesnark|afkone thing before I go, my local ISP just started offering 110MBps connections07:29
thesnark|afktotally contemplating it07:29
katsmeow-afksome of my mining has kept running for 7 months, at 2 hits per second, lot of garbage urls tho, it wasn't all good07:30
katsmeow-afkthesnark|afk, you cannot hit most pages any faster than slow dsl, or they'll throttle you07:30
ParahSailin_thiel didnt do shit until he was like 2807:30
ParahSailin_so dont feel bad07:31
thesnark|afkkatsmeow if I can't go depth first I'll go breadth first07:31
thesnark|afkmany sites at once07:31
katsmeow-afkagreed07:32
kanzureParahSailin_: thiel is not a good role model :P07:32
thesnark|afktime to go away for real now07:32
katsmeow-afkhave funs07:33
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Mokbortolan_http://scienceblogs.com/neurophilosophy/2011/03/gut_bacteria_may_influence_thoughts_and_behaviour.php08:51
kanzureMokbortolan_: i hear that you are female08:51
kanzurecan you confirm/deny?08:51
Mokbortolan_That's an interesting rumor08:51
kanzureyes i thought so too08:51
Mokbortolan_I am decidedly "cis-male"08:51
kanzureahuh08:52
kanzuremy source is bad :)08:52
* Mokbortolan_ strokes his neckbeard.08:52
Mokbortolan_I am intrigued, however, as to the source of this08:52
kanzuresteel3|08:52
Mokbortolan_Hah!08:52
Mokbortolan_Maybe he's got me confused with the lady who runs MyModafinil.com08:52
Mokbortolan_Steel2: Explain yourself sirrah!08:53
Mokbortolan_I'm not offended though, women can be wonderful people08:54
Mokbortolan_why, if I were a woman I'd marry me immediately08:55
Mokbortolan_not sure how the wife would react, but I'm sure that would sort itself out over time08:56
Mokbortolan_I'd have to lose some weight, and do something with my hair, however08:58
Mokbortolan_And not be so argumentative.  Crimony woman, it's like living with the captain of the Asperger's Society Debate Team!08:59
d3nd3loooooooooooooooooooooooooooooool +109:00
phrykWhat's the "cis" in "cis-male" stand for? o_O09:00
Mokbortolan_it's a silly convention I recently learned of09:00
Mokbortolan_basically, it means that one is "gender normative"09:01
Mokbortolan_so I'm male in the Ernest Hemingway tradition, as opposed to the Herbert Hoover tradition.09:01
phryk"gender normative" meaning being heterosexual and not into bdsm?09:02
Mokbortolan_it's more about identifying with your gender09:02
Mokbortolan_"a man in a man's body", so to speak09:02
phrykAh.09:02
Mokbortolan_err, not herbert hoover09:02
Mokbortolan_sorry Herbert09:03
Mokbortolan_I meant J. Edgar Hoover09:03
phrykNow I'm wondering if I'm cis-male…^^09:03
Mokbortolan_you know, cis/trans?09:03
Mokbortolan_chemistry term09:03
phrykI don't really give a damn about gender… I feel okay being male, but I think I'd feel okay as woman, too…09:03
phrykNo, sorry.09:04
Mokbortolan_you should watch this great movie, "trans-america"09:05
kanzurehe means "manly man"09:06
Mokbortolan_oh sure, if you don't want to use vague and confusing terminology09:07
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thesnarkpeople overcomplicate sexuality so much09:16
thesnarkfind a person, pleasure each other's organs until both have orgasmed09:16
thesnarkthe end09:16
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Mokbortolan_Abandon All Culture, Ye Who Enter Here09:23
rkosall hail the numbers09:29
delinquentmeall hail nombers09:30
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ybit21:26 < kanzure> "Recommended online education sources were: GetHub, Instructables, and Hack A Day"12:07
kanzureit was from chicago opensci's group meeting notes12:07
ybitat least he's taking note of version control12:07
kanzurehttps://jrvarma.wordpress.com/2010/04/16/the-sec-and-the-python/12:31
kanzurehttp://www.itworld.com/government/105031/will-wall-street-require-python12:32
kanzure"We are proposing to require that most ABS issuers file a computer program that gives effect to the flow of funds, or “waterfall,” provisions of the transaction. We are proposing that the computer program be filed on EDGAR in the form of downloadable source code in Python. … (page 205)"12:33
kanzure"Under the proposed requirement, the filed source code, when downloaded and run by an investor, must provide the user with the ability to programmatically input the user’s own assumptions regarding the future performance and cash flows from the pool assets, "12:33
kanzure"ncluding but not limited to assumptions about future interest rates, default rates, prepayment speeds, loss-given-default rates, and any other necessary assumptions … (page 210)"12:33
kanzure"The waterfall computer program must also allow the use of the proposed asset-level data file that will be filed at the time of the offering and on a periodic basis thereafter. (page 211)"12:33
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jrayhawkhttp://jackkruse.com/cold-thermogenesis-7/ hooray, he's become more coherent13:30
kanzureartisan's asylum looks ok13:36
kanzurehttp://artisansasylum.com/?page_id=1813:36
kanzurebut it's a little strange that they don't have things like.. a laser cutter.13:36
kanzureapparently this is one of the boston dynamics guys13:37
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kanzurebacterial radio thing14:34
kanzurehttp://www.biofaction.com/synth-ethic/?p=4414:34
kanzure"Bacterial Radio exhibits several bacterially-grown platinum/germanium electrical circuits (crystal radios) on glass substrates. Joe Davis, in collaboration with Ido Bachelet and Tara Gianoulis from Harvard Medical School in Boston,"14:35
kanzure"used bacteria altered with variants of a gene from orange marine puffball sponges (Tethya aurantia) to plate electronic circuits on Petri dishes and microscope slides."14:35
kanzure"This gene codes for a protein – silicatein – that normally forms Tethya aurantia’s glass skeleton, its tiny, glass, needle-like spicules composed of silicon and oxygen. Variants of this gene have now been optimized to plate metallic conductors and semiconductors including germanium, titanium dioxide, platinum and other materials."14:35
kanzure"Here, genetically-modified bacteria are embedded in non-conductive materials containing metal salts, and then optically induced to plate specific, electrically conductive circuits."14:35
kanzure"These Bacterial Radios on display are connected to high impedance telephone headsets, antennae and ground, so that visitors may use them to actually listen to AM radio broadcasts."14:35
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AdrianGlol14:37
Steel3?14:38
Mokbortolan_Steel3: I heard you were a girl!14:39
Steel3Wat.14:39
Steel3no, lichen and molybdenum are the girls14:39
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Mokbortolan_no no no14:39
Mokbortolan_I meant, "were" a girl14:40
MariuI got CPUs of steel14:40
AdrianGkanzure: can u control those bacteria via radio?14:40
Steel3wat.14:41
Steel3someone said I'm transgender?14:41
Mokbortolan_:p14:41
Steel3lmaoing.14:41
Mokbortolan_j/k, who told you I was of the feminine persuasion?14:41
Steel3I didn't say anything about you being feminine14:43
Steel3?14:43
Steel3I said molybdenum not mokbortalon14:43
kanzureuhuh14:44
kanzurestill sounds just as unlikely14:44
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Steel3what's the point of being skeptical about this?14:45
Steel3I mean, shit, I have moly on facebook. But why not address someone with their preferred pronoun?14:46
kanzurei have nothing against pronouns14:46
kanzureit's just unlikely14:47
Mokbortolan_yeah14:47
kanzureExtraordinary claims require extraordinary evidence, and such14:47
Mokbortolan_everybody knows there are no girls on the internet14:47
kanzureyep14:47
Mokbortolan_only pictures of them14:47
Steel3but this isn't really an extraordinary claim to give someone shit over14:47
kanzurei'm not giving youshit o.o14:47
Mokbortolan_I am, but it's all in good fun14:47
Steel3not me, I'm a dude14:47
Steel3whatever, I can take it14:47
Steel3however, the culture of proof or gtfo (with regards to something is irritatingly pointless as gender) isn't the most welcoming14:48
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kanzurei never said gtfo for this reason14:49
Steel3apologies, hyperbole.14:49
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Replopso before acquiring balls of  Steel , there were boobs of Steel ?   I see ....14:56
Mo|ybdenumwhat?14:56
Mo|ybdenumSteel's uh...always... been a dude.14:56
Steel3rumors, jokes, etc. apparently abounding14:56
Replopnot according to Mokbortolan_14:57
Steel3Mokbortolan_'s a cock :P14:57
strangewarpPretty sure I would prefer to have no gender at all, once I can.14:57
Mo|ybdenumEveryone loves to think I'm a dude so meh.14:58
Replopstrangewarp:   so you would give up the possibiliy of  sex as a recreational activity ?14:58
katsmeow-afkno gender or no sex?  you cannot choose gender, i think, but you can surgically reassign sex14:58
strangewarpReplop: Yes. I already get grossed out by the idea of any kind of sex, so it's no big thing14:59
jrayhawkgender identity appears to be epigenetic, so we'll probably be able to modify it eventually14:59
katsmeow-afksocieties pretty demand you fit some form of gender, regardless of your sex14:59
Replopor go the Snails way and become hermaphrodite;  so people that want to reasign their sex won't need surgery anymore14:59
strangewarpNo gender, no sex, no sex drive, etc.14:59
kanzurejrayhawk: gender modification tech is shockingly primitive14:59
kanzureor i guess advanced compared to the other boneheaded medicine we have14:59
katsmeow-afkintersexed (hermaphrodite) is also a pretty broad term, iirc there's 16 variations15:00
jrayhawkwell, genetic methylation is a fairly recent discovery; i think we should give medicine some time15:00
katsmeow-afkconsidering some women take testosterone to increase sex drive, but don't become male or men, i dunno how you'd change genders, even if you did chage their sex15:01
Mo|ybdenumSRS for male to female is fairly well off, but female to male is still extrememly rudementary.15:01
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jrayhawkand i suppose gender identity is a political landmine, so research will lag for a while on it15:01
Mo|ybdenumrudimentary? spelling.15:01
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katsmeow-afkrudimentary, check, correct15:02
Mo|ybdenumyes but gender is the mental construct so hormones wouldn't nessecarily effect that.15:03
katsmeow-afki believe that is correct15:03
katsmeow-afkeven applying the wrong hormones prenatally has been shown to cause transsexualism, not transgenderism, in humans15:04
ThomasEgihm. hormones could affect mentality too.15:04
katsmeow-afkthey can affect agression, but not which sex you want to have sex with15:04
ThomasEginot so sure.15:05
Mo|ybdenumHasn't it been shown in cases where hormones effected gender predilections the person already had leanings towards those things to begin with.15:05
katsmeow-afkyeas15:05
ThomasEgidifficult subject i guess. with mand influences overlapping each other. i would like to pretend all combinations are possible15:05
ThomasEgias there is no way to definetly rule out one or the other15:06
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katsmeow-afkhuman experimentation of tis type is frowned on15:07
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Mo|ybdenumeven if it wasn't you still have societal mores/norms to contend with15:08
katsmeow-afksociety would frown on it15:09
Mo|ybdenumat least in america you have the whole religious right and their insanity.15:09
Mo|ybdenumnot even going into cultural hold overs of "manly men" and "fragile women"15:10
kanzurei regret bringing this up15:10
katsmeow-afkmany societies are onordinately preoccupied with another's gender, and even persoanlly involed id-wise if there's any attraction involved in any way15:10
Mo|ybdenumexactly.15:10
Mo|ybdenumwhich is kind of bizarre when you think about it.15:10
katsmeow-afkvery15:10
Mo|ybdenumi'm sure there is some psychological reason for that15:11
ThomasEgisocietly is bizarre an many aspects. especially since it blocks new creative and potentially more productive ways of thinking15:11
Mo|ybdenumsome of that can be blamed on religion, but not all of it.15:12
ThomasEgihehe.. but all of it can be blamed on the humans^^15:12
katsmeow-afkconceit, i imagine,, figuring if anything oes wrong it's either "they did something wrong to me"  or "i was stupid enough to be suckered"15:12
katsmeow-afkand it may not be factual, only percieved15:13
Mo|ybdenumYep, it is definently all humans' fault, it's just that often times the preconcieved notions are based on ' (holy text here) says it's wrong"15:13
katsmeow-afkeven if only percived by others: "i don't care, but others will make fun of me, so i haveto protect my honor"15:13
Mo|ybdenumexclusion is a powerful thing.15:15
katsmeow-afkyeas, growing up on a farm and seeing cows, rabbits, birds, dogs, cats, yoou name it, having same-sex fun, and then sunday hearing the priest saying only human are gay and because of "the devil", religion is lying15:15
Mo|ybdenumYeah it's gonna be a while before that really gets thru to alot of people.15:17
katsmeow-afk2000 yrs, ithasn't happened yet, too many people have a need to be followers15:18
ThomasEgithe "devil" is pretty much the weirdest concept i encoutered in human argumentation. usualy used to prevent other people from doing things you dont like.15:18
katsmeow-afknah, the dicotomy is common in many thing, opposing forces all over the place15:19
Mo|ybdenum"You're gonna go to hell!" is another  one.15:19
katsmeow-afkit's the whole bit about a potential ruler of a country claiming he has the strongest belief in his imaginary friends, and the most willingness to do as his preacher tells him to do, that scares me15:20
ThomasEgireminds me of that one definition of holy-wars. "the fight between grown up men about who has the coolest imaginary friend"15:21
katsmeow-afkin many ways, the usa is the xtain version of Iran or Pakistan15:21
katsmeow-afkyep15:21
strangewarpkatsmeow-afk: That's actually pretty true. Both the US and Iran had their quietist religious fundamentalists activated as political groups in the 1970s.15:21
kanzurewut?15:21
kanzureOn Wed, Mar 21, 2012 at 4:58 PM, Eric Mossotti <edaptmogen@gmail.com> wrote:15:21
kanzure> The Venus Project needs experienced people to volunteer some time,15:21
kanzure> regarding possibly transitioning into the Open Community..15:21
kanzure>15:21
kanzure> www.thevenusproject.com15:21
kanzure> www.tvpactivism.com15:22
kanzure>15:22
kanzure> I have been discussing this in a thread (link below), and am to the point15:22
kanzure> that people who have done open projects, or related things, need to input15:22
kanzure> into this. I have done a bit of research and am needing anyone who can15:22
kanzure> possibly have some correspondence with me and maybe show how things will be15:22
katsmeow-afkcharacterising the Taliban, for instance, as a war-making terrorst org, when the usa hasn't been out of a war in 100 years, is ludicrus15:22
kanzure> handled if the choice is made to go Open with past, present, and/or future15:22
kanzure> works and projects.15:22
kanzure> I think this volunteer organization is very much aligned by default, by15:22
kanzure> definition, with the Open and Free Software and related movements, however15:22
kanzure> the person who started this is in his mid 90s now, is still alive and well15:22
kanzure> for his age, but doesn't know about these things. I am trying to bridge the15:22
kanzure> gap between us and the all encompassing open developments that are growing15:22
kanzure> worldwide.15:22
* katsmeow-afk ponders on stomping on kanzure's next conversation without waitng for a pause in that conversation15:24
kanzurekatsmeow-afk: you're perfectly welcome to15:24
kanzurethis is an irc channel.. it can handle more than one conversation at a time15:24
katsmeow-afkit would be impolite of me to do so <cough>15:24
kanzurei am a very impolite person15:24
* katsmeow-afk nods15:25
Mo|ybdenum*blinks a few times*15:25
Mokbortolan_I think it's funny how quickly people like to dismiss the profound differences between men and women15:26
Mo|ybdenumand that was a good conversation too :(15:26
Mo|ybdenumprofound differences?15:26
Mokbortolan_in the brain, especially15:26
Mokbortolan_although I guess in this case "profound" is a matter of degree15:27
katsmeow-afki tend to think many of those are imposed while growing up tho15:27
kanzurebillions and billions15:27
katsmeow-afknot all, but many15:27
Mokbortolan_(male vs female) vs (male vs male)15:27
Mokbortolan_katsmeow-afk: I'm really referring to the white/grey matter differences15:28
katsmeow-afki wis the edges could be taken off , and poele would stop trying to live on the fringes of the bell curve of gender-steriotype behaviors15:29
Mokbortolan_and from what I've read, post-natal changes to that are few15:29
katsmeow-afkproblem is the brain is programmable over the years, and with it comes some physical changes15:30
Mokbortolan_problem?15:30
Mo|ybdenumi think Kat means in the sense of saying there is this hardline difference15:30
katsmeow-afkand in some case, physical attributes of the local environment play a huge part: consider home ec classes, body size15:31
Mokbortolan_they're not the same15:31
Mokbortolan_male and female brains are not the same15:31
Mokbortolan_but, what does that mean? what are the substantive differences?15:32
katsmeow-afkthere is some hardline differences, crack-addicted babies are prone to addiction to anyting the rest of their lives, and may well become addrenaline junkies and act out in behavoral ways15:32
katsmeow-afkso it's a small leap to saying otehr chemical soup difference neonatally can have some difference15:33
katsmeow-afkbut the human should be smart enough to minimise a lot of that15:33
Steel3hard line differences are hard to separate from societal condition differences, given that the needs of our environment shape the brain15:33
kanzureall of this is boring let's just sacrifice katsmeow-afk and scan in her brain15:34
kanzurethen we will have real answers15:34
katsmeow-afki agree, but there are cases where a 120lb woman wants to be a heavyweight boxer, and the 250lbs 6-4 man wants to be a woman15:34
katsmeow-afk"want" to be taken with a grain of salt15:35
Mokbortolan_I don't really have an argument, except to say that people who maintain that men and women are exactly the same do so out of allegiences to something other than reality15:35
katsmeow-afkanyhow, i do need to go afk, and kansure may have a cow if i don't, regardless if the rest of you continue the conversation15:36
Mariukanzure, what about advanced sensors that scan her brain as alternative ?15:36
kanzureMariu: what sensors?15:36
Mariukanzure: don't know15:36
* Mokbortolan_ looks up brain scans of trans- people.15:36
Steel3Mokbortolan_: not exactly the same, but we can't tell which differences are innate and which are social (for a developed brain)15:36
kanzurewhy would that matter?15:37
Steel3Because it would indicate where 'differences between men and women' are (at least attitude/personality/brain wise) more a matter of biology or how they're treated culturally15:37
kanzureneuroplasticity is a fact15:38
kanzureMokbortolan_ was mentioning that there are genetic brain differences15:38
kanzurebut, there are genetic brain differences between all of us15:38
Mariu^15:38
Steel3And we don't know what those are unfortunately, as far as I know15:38
kanzuregenetic brain differences are easy to tell15:39
kanzurejust scan for the known brain snps15:39
Mokbortolan_women have more interconnects and smaller processors, men have bigger processors and fewer interconnects15:39
strangewarpIf you don't like your brain, change it15:39
kanzureMokbortolan_: "smaller processors"?15:39
kanzurewhat15:39
MariuLOL15:39
Steel3Mok: Do you know for sure that's biological and not social?15:39
Mokbortolan_Steel3: I'm pretty sure15:39
kanzureare any of you neuroscientists or am i pissing in the wind here?15:39
MariuMiniature versions15:39
Mokbortolan_I'm an armchair neuroscientist15:39
Steel3http://news.bbc.co.uk/2/hi/677048.stm eg.15:40
Mokbortolan_I get all my information from Time magainze15:40
Mariuwould be nice to design your own brain15:40
kanzurethen do it15:40
kanzurenobody is stopping you15:40
Mokbortolan_except that pesky reality15:41
kanzurewhat?15:42
kanzurehow?15:42
Mokbortolan_Steel3: the taxi drivers grew a section of their brain, they didn't completely lay it out differently15:42
kanzurethere is nothing stopping Mariu from "designing a brain"15:42
Steel3point is we don't know what the extent of cultural vs biological is15:42
kanzureSteel3: neuroplasticity is still a fact.. "culture" doesn't change that15:42
Mokbortolan_kanzure: you're right15:43
Steel3Right! But we can't tell which differences between men and women are caused by what15:43
Mokbortolan_implementation, however, is another matter :p15:43
Steel3or at least not well15:43
kanzureMokbortolan_: hee he didn't specify that15:43
Mo|ybdenumthe only way you'd be able to figure out what is and isn't culturally based would be raising someone in seclusion and that's all kinds of human rights abuses.15:43
Mokbortolan_Steel3: teh differences can be seen at 26 weeks of fetal development15:44
kanzureMo|ybdenum: not true.. you can test neural tissue cultures and figure out which modes of plasticity are available under which conditions15:44
Mokbortolan_ostensibly before culturally-induced changes can occur :p15:44
Steel3doesn't help much with full developed brain15:44
Steel3partially, yes15:44
Mokbortolan_http://www.ncbi.nlm.nih.gov/pubmed/2060078915:45
kanzureif you are going to claim all this nonsense about culture/social,15:45
Mariuyou could do it through bio-hacking15:45
kanzurethen look at other animals15:45
kanzureand stop complaining to me about this15:45
Mariu*biohacking15:45
Mokbortolan_Mariu: I'd settle for simpler organs15:46
Mokbortolan_lots of folks out there needing kidneys, after all15:47
kanzureoh that's what i should have them do15:47
kanzureSteel3: i should have them make a black market app for organs15:47
Steel3hah15:47
Mokbortolan_or, barring that, I'd like a way to tack additional brains onto my own15:47
Mokbortolan_although, that would make finding a hat that fits much more difficult15:49
Mariuan engineered brain will problably made out of modules, each module taking certain functions of the brain15:49
Mariueach module handling15:50
Mariu*15:50
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* katsmeow-afk does a flyby15:59
katsmeow-afkraising someone in seclusion won't help, because all the tasks for survival that are gender specific in a society would need to be done by the one person for them to survive16:00
Mariuyou upload the tasks16:01
katsmeow-afkcuttng firewood, killing animals for food, home construction,, or gardening, cooking, clothes mending, cleaning16:01
Mariukilling animals ??16:01
katsmeow-afkok, eating them live16:02
Mariuthat's not modern at all16:02
Mariunot even civilised16:02
* katsmeow-afk looks for where she said "modern"16:02
Mariuimagine having a species using you as food material16:03
MariuI bet you would like that16:03
katsmeow-afki didn't say i agree with it16:03
katsmeow-afki recently discovered i have been dietarily protien deficient, but instead of killing animals, i am buying fortified whey protien powders, so please get off my back16:04
Mokbortolan_that whey comes from somewhere16:05
Mokbortolan_probably cows16:05
katsmeow-afki grew up on a farm, as previously stated, and was several times forced to eat part of one of my non-human friends, i severely didn't like that16:06
Mariu*civilized16:06
Mokbortolan_yeah, probably not a good idea to befriend the livestock for that reason16:06
Mokbortolan_hard not to do though when you're a kid16:06
katsmeow-afkit's a fact humans must eat, but at least i am not killing the cow16:06
Mokbortolan_I've always been of the opinion that the closer you are to the animal, the better16:07
Mokbortolan_to help you be grateful and respectful rather than flippant, tossing out meat that wasn't spiced to your liking16:08
katsmeow-afkdoes anyone with knowledge know if the whey powders are a suitable offset for not eating cows and pigs and rabbits and chickens and etc?16:09
Mokbortolan_in a dietary sense?16:09
* katsmeow-afk nods16:09
Mokbortolan_no16:09
katsmeow-afkwhat's missing, and what do i do about it?16:10
Mokbortolan_you'll need b12 and vitamin d at minimum16:10
katsmeow-afkthat's in a vit pill16:10
Mokbortolan_I was a strict vegetarian for a while16:10
Mokbortolan_not really16:10
katsmeow-afkingredient list says it is16:10
Mokbortolan_bioavailability is an issue16:10
Mokbortolan_and you're a female, right?16:10
katsmeow-afkthat's hard to find out about from a pill company16:10
* katsmeow-afk nods some more16:11
katsmeow-afkbut, why would that matter?16:11
Mokbortolan_you'll also need iron, and no, supplements are not a good substitute16:11
Mokbortolan_as my wife found out16:11
katsmeow-afkwell, some epople do not absorb stuff same as other epople16:11
Mokbortolan_women need more iron than men16:11
Mokbortolan_this is true16:11
Mokbortolan_men only lose iron when we cut ourselves :p16:12
katsmeow-afkwithout blood testing, there's not a good way to see if you aren't absorbing, sort of a deficiency disease or disability,, or death16:12
Mokbortolan_there's a quick test for anemia16:12
Mokbortolan_not precise, but accurate16:12
katsmeow-afkiron isn't lost in blood cell death?16:12
Mokbortolan_no, it gets recycled by the spleen16:13
katsmeow-afkhmm16:13
Mokbortolan_flip down your lower eyelid, if the tissue there is nice and red, you're fine16:13
Mokbortolan_if it's pale, you're anemic16:13
katsmeow-afki'll chec later when i have a mirror handy16:13
Mokbortolan_it's really hard to substitute animal flesh (long term) in the human diet16:14
Mokbortolan_you can do it for a long time, no doubt16:14
Mokbortolan_or, some people can16:14
Mokbortolan_but at a cost16:14
MariuMoby did it16:15
katsmeow-afkyeas,, i was running into that on the protein issue16:15
Mariu... the singer16:15
Mokbortolan_protein isn't really an issue though16:16
Mokbortolan_there's tons of protein in vegetable foods16:16
katsmeow-afkafaik i had all the B vits and otehr stuff,, prolly a cholesteral imbalance16:16
katsmeow-afkyeas, but veggies come with calories, and often cost more than grains16:16
Mokbortolan_and if you're gluten intolerant, you've got some serious issues being vegan :p16:18
Mariuyou design your body to be gluten tolerant16:18
katsmeow-afki am often pissed that my garden veggies are coverd with flecks of ash from someone's burning garbage16:18
Mokbortolan_Oh do I?16:18
Mo|ybdenumyeah, I know all about gluten intolerance and lactose intolerance.16:18
* katsmeow-afk eats breads just fine16:19
Mokbortolan_eww16:19
Mokbortolan_live out inthe country, I take it16:19
katsmeow-afkyou don't like bread?16:19
katsmeow-afkyeas16:19
Mokbortolan_eww to the ashes16:19
Mo|ybdenummore like bread doesn't like me.16:19
katsmeow-afkfresh country air is often far more polluted than the city air16:19
ThomasEgiMo|ybdenum, be glad it is only gluten. mate of mine had fructose intollerance.16:20
Mo|ybdenumO_o how do you live being allergic to fructose16:20
katsmeow-afkgas prices in some way reflect the chemistry in it to modify emmisions, yet the epople driving the cars burn garbage, and there's some seriosly toxic stuff from buring plastics at low temperatures16:20
ThomasEgihe could count the dishes he could eat on one hand16:21
kanzureah yes the mythical fructose-free fructose16:21
ThomasEgiwell. you pretty much have to eat meat, cooked, with no spice and nothing.16:21
ThomasEgithere are a few ingredients that are low on fructose16:21
katsmeow-afkno garlic or tumeric?!16:21
ThomasEginothing. execpt meat , cooked in (i think olive oil?)16:22
ThomasEginot sure.16:22
Mo|ybdenumor paprika probably.16:22
ThomasEgiand rye bread iirc16:22
katsmeow-afkouch16:22
ThomasEgiafter a year he was able to eat banana. no idea what's low fructose bout them..16:22
ThomasEgiwell after 4 years in total. he's now back to normal.16:22
ThomasEgistrange body he has. but fructose intollerance runs in his family tree.16:23
katsmeow-afk"set that replicator to 'low fructose'"16:23
katsmeow-afkgood thing there's other sugars16:23
katsmeow-afkanyhow, i need to afk again16:23
ThomasEgitime for bed for me too.16:23
kanzurehttp://ipod.com/init16:24
kanzureph33r16:24
Mo|ybdenumis dat sum phr34king16:25
kanzurehttp://pastebin.com/yZxWJD9n16:27
kanzureif it 404s.16:27
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jrayhawkFWIW nobody eats bread fine; the CXCR3/zonulin cascade is bad news.17:04
kanzurei wonder what the synthesis steps are for modern contraceptives17:05
jrayhawkWhey contains a bunch of IGF-1, which is a nondiscriminating growth promotor.17:05
jrayhawkIt is a good idea when you are a child and need a lot of nonspecific growth, it is somewhat more problematic when you are an adult.17:05
jrayhawkCarnitine and the various B-vitamins are difficult to get from non-meat sources, so you should worry about those.17:06
jrayhawkYou should also worry about long-chain omega-3; terrestrial plant life simply will not cut it.17:07
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jrayhawkEggs are a better option than whey; go for free-range/pastured eggs to maximize omega 317:07
jrayhawkand eat a shitload of saturated fat to make the most of whatever long-chain PUFA you can get17:08
jrayhawkThe big problem with eggs is that eggwhites contain avidin which only goes away with high temperature cooking, but high temperature cooking oxidizes PUFA17:09
jrayhawkthey're a pretty crap protein source, but they're still less awful than whey.17:09
jrayhawkI've lately just taking to tossing the whites and eating the yolks raw.17:09
jrayhawks/taking/taken/17:09
jrayhawkIf you're okay with it morally and can manage to stomach it, http://www.greenpasture.org/public/Products/CodLiverOil/index.cfm will take care of most of your A/B/D vitamin problems and omega 3 intake17:11
jrayhawktropicaltraditions.com sells bucks of saturated fat; the expeller-pressed variety is essentially tasteless.17:12
jrayhawks/bucks/buckets/17:12
kanzurewoulda liked bucks.. gotta hunt down those animals17:13
jrayhawkI guess I should qualify my CXCR3/zonulin snipe a little better...17:15
jrayhawkI'm not suggesting you have coeliac; intestinal permeability is a separate but related issue. Coeliac is an acute inflammatory and often painful reaction (that some people have) to lymphatic infiltration by grains (which seemingly everyone has to varying degrees).17:15
jrayhawkBeing born without coeliac is like being born without pain receptors. Just because you don't get acute inflammation and pain when punching yourself in the face doesn't make it a good idea.17:16
Mo|ybdenumwould pea or hemp protein be an option?17:24
Mo|ybdenumbecause i don't do wheat or dairy.17:24
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jrayhawkFWIW fermentation often gets rid of problematic compounds, so your options can expand somewhat with that in mind.17:38
kanzurehmm the openscad guy is commiting to scl now17:38
kanzure*committing17:38
kanzure"Hi guys - we've started working on the BRL-CAD SCL/github SCL syncing effort, and as part of that I would like to ask if the following patch could be applied to the github CMake files?"17:38
kanzurewell i'm glad brlcad is integrating with scl again17:38
kanzuretoo bad nist isn't doing what they're supposed to17:38
kanzureoh wait17:45
kanzureclifford yapp is starseeker, not clifford wolf17:45
kanzureso no, not the openscad guy, the brlcad guy :)17:46
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kanzurehttp://marblar.com/2012/03/dna-click-ligation/17:56
kanzurehttp://marblar.com/wp-content/uploads/2012/03/copper-free-click-ligation-page-nos-chem-comm-2011.pdf18:00
kanzureso obviously the company sponsoring the competition wants to steal all the "IP"18:17
kanzure"Edited by Jack W. Szostak" haha in other words 'shit is going down'18:25
kanzurepage 2 has a better diagram of this dna ligation chemistry: http://marblar.com/wp-content/uploads/2012/03/triazole-DNA-PNAS-with-page-Nos.pdf18:26
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kanzureyashgaroth: logs..18:30
yashgarothwhoa now I'm not diving into the male/female debate18:31
kanzureno.. the last thing18:31
kanzurepage 2 http://marblar.com/wp-content/uploads/2012/03/triazole-DNA-PNAS-with-page-Nos.pdf18:31
kanzurei haven't found the yield yet18:33
yashgarothit's an interesting idea...if you can get a linker that's more efficient for synthesis than pamidites, 'twould be awesome18:37
kanzurei don't think anyone uses phosphoramidites to link together 100mers18:38
kanzureyashgaroth: have you seen otherwise?18:38
d3nd3kanzure: hi18:39
yashgarothI'd imagine people just use overlap extension pcr for that18:39
kanzurei think there's a technical reason that you can't join 100mers with phosphoramidite chemistry18:39
yashgarothefficiency would be total shit18:40
yashgarothnot to mention self-ligation and all that18:40
kanzurewhy would there be self-ligation?18:40
kanzureone end of your free-floating 100mer would be capped, another would be free to bind with valid uh.. targets18:41
yashgarothdepends how you're doing the reaction, but there's no real reason to attempt ligating them when you can take the 3bp overlap hit and just use pcr18:41
kanzure3bp overlap is enough for extension pcr?18:42
kanzurewhat? i thought it needs to be more like.. 10-1518:42
yashgarothyou'd probably want more, but still, it's easier than ligating them at the very end with pamidite chem18:42
yashgarothI wonder if it'd be possible to evolve a polymerase that would recognize an entirely artificial backbone18:50
yashgarothi.e. a backbone with chemistry more amenable to long strand synthesis18:50
katsmeow-afkdamn, i thought yo were gonna make me a new lumbar spine18:51
fennmeep18:52
kanzurehi fenn18:53
kanzurefenn: the solution was "login again"19:08
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kanzure"Don't try to construct the future like a building, because your current blueprint is almost certainly mistaken."21:09
kanzureMY BLUEPRINT IS PERFECT21:09
kanzureprovably so! in haskell21:09
delinquentme^21:11
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n_benthahey...21:24
n_benthaanyone done transformation recently?21:24
yashgarothdoes yesterday count21:24
n_benthathe protocol i was using hasn't been going so well.21:24
yashgarothdeeeetails21:25
n_benthayes yashgaroth! i was going to do one today, but didn't cuz some retard who was supposed to have the bacterial cultures ready didn't.21:25
yashgarothoh god you're not making your own competent cells are you21:25
n_benthawell it was the cacl2 method, but from some ancient book and it had lots of centrifugation steps and a really short heat-shock time, and long ice-times21:25
n_benthayes yashgaroth i was21:25
yashgarothyou poor bastard21:26
n_benthatell me about it. stupid grant doesn't come in for another few weeks21:26
yashgarothso you're not getting any colonies or what21:26
n_benthawell i got some colonies when i tested the cells for efficiency--but not many on the plate21:27
n_benthaand i froze them and then tried them later, but they didn't work. then i tried to use an old stock and that didn't work either.21:28
n_benthaso I saw this today: http://tequals0.files.wordpress.com/2012/03/p1010516.jpg21:28
n_benthacould I just go ahead w/ that protocol and not make a whole batch of aliquoted competent cells?21:29
yashgarothneeds an ice step after the heat shock21:29
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yashgarothalso it sounds counterintuitive put try using less DNA21:31
yashgarothbut*21:31
n_benthaso after the initial 15 minutes on ice w/ dna, there's a 5 minute step. is that just room temp before the heat shock?21:32
yashgarothgod no, go straight from ice to the 4221:32
yashgarothalso if you are making your own cells, make sure they're in exponential growth21:32
yashgarothice after heat shock is like 30seconds btw21:34
n_benthaya that was probably the problem. stupid lab mate let them shake too long.21:34
n_bentha30s? No wai! It was longer on the protocol I had.21:34
yashgarothehh you can do a few minutes, I don't usually bother21:35
n_benthaI guess I should go ahead and centrifuge the cells when I pull them out of the shaker, right?21:35
n_benthaShit nevermind. Stupid me21:35
yashgarothya don't pellet cells you don't plan on lysing21:36
n_benthaI'll take the colony off the plate, stick it in the 0C cacl2 (250ul), vortex, add DNA, let it chillax for 15min, then heat shock for 90seconds, then ice for 30 and plate?21:37
n_benthaoh 250LB and then plate21:38
n_benthaSound good?21:38
n_benthaAnd add a little less than 10uL DNA21:38
yashgarothI'd recommend a gentle pipetting rather than vortex, and SOC rather than LB, if you have it21:39
n_benthasure.21:39
yashgarothand make sure the rescue media is prewarmed, natch21:39
kanzureyashgaroth: wait why wouldn't he make his own competent cells?21:39
kanzurewhat's the alternative?21:39
n_benthaorder21:39
yashgarothpremade cells from...invitrogen's what we use, I think21:39
n_benthayeah, that's what i used to use21:40
fennkatsmeow-afk: i agree with jrayhawk's nutritional advice. another vegetarian protein concentrate is pea protein, about $14/lb. most days i eat a 15 oz can of sardines for breakfast21:40
n_benthahmmm...warm lb. it's got what cells crave21:40
yashgarothit *does* have electrolytes21:40
yashgaroth10g/L if I recall21:41
fennwhy is it so hard to make your own competent cells?21:41
fenni mean, aside from not having correct instructions21:41
n_benthabad voodoo, fenn21:41
yashgarothit's not, but many people prefer the reliability of blaming someone else if it goes bad21:41
fennjust have your robot do it 50 times21:42
n_benthayashgaroth, my competent cell protocol had a bunch of centrifugation steps. u think that's what f'd things up along w/ cell not in the growth phase??21:42
fenn(what do you mean you dont have a robot?)21:42
yashgarothyou're sticking pelleted cells into the cacl2?21:42
n_benthawe couldn't afford robots, so we hired some undergrads21:42
n_benthathat's what the protocol i was told to follow said, yash21:42
n_benthaI said literally to my over-lord, "why the fuck do we have to pellet these cells?"21:43
yashgarothI learned it with suspension cells, but like much of bio, it's all art and little science21:43
yashgarothanyway that's probably not where your problems are occurring21:45
n_bentha"To prepare competent E. coli a culture is grown and then harvested when21:45
n_benthait is in log phase, at which stage the bacteria are dividing rapidly. The cells21:45
n_benthaare harvested by centrifugation and washed several times in a chilled21:45
n_benthabuffer containing divalent cations, typically CaCl2. The bacteria are finally21:45
n_benthasuspended in a small volume of the buffer so that they are present at a high21:45
n_benthadensity."21:45
yashgarothhow did the protocol you were using differ from that drawing you linked?21:46
n_benthaIt had me pellet out and re-suspend the cells multiple times before adding the CaCl221:47
yashgarothbut you're still resuspending in cacl2, no?21:48
n_benthaand then adding the DNA after the CaCl2 step.21:48
n_benthahmm I'd have to check. I think it was just re-suspending in some buffer?21:48
yashgarothdid you do a mock transformation with no selection on the plate? was it a lawn?21:50
yashgarothif you're getting less than thousands, then it's your cell health21:51
n_benthano, just used the antibiotic plate21:52
n_benthaok cell health it is21:52
n_benthastupid cells.21:52
n_benthathis protocol says to just use heat: http://www.ucc.ie/ucc/depts/physio/MolPhysiol/MolPhys/Protocols/pdfs/014.pdf21:52
n_benthaIf that flow-chart method doesn't work this friday, I'll try w/ just heat.21:53
yashgaroththat still uses competent cells21:54
n_benthaoh god-damnit21:54
n_benthasorry yashgaroth.21:55
yashgarothmmm also which antibiotic are you using?21:55
n_benthakanamycin21:56
yashgarothoh damn make sure you do an hour incubation in the rescue media before plating21:56
yashgarothyou can't go straight to the selection plate unless you're using amp etc.21:57
yashgarothkan'll kill them before they have a chance to express the resistance gene21:57
n_benthaI love you.21:59
yashgarothd'aww21:59
n_benthaI'll let you know how it goes next week :)22:09
yashgarothgood good22:09
fennn_bentha: how much would you be willing to pay for a lab robot?22:10
kanzurei think yashgaroth would be a better person to ask22:11
kanzureyashgaroth: how much could your company save by replacing all that manual labor22:11
n_benthaHow much would I be willing to pay or how much would my PI be willing to pay? And ya, kanzure is right.22:11
kanzuren_bentha: both22:12
n_benthaFenn, does the robot just transform the dna, or make competent cells as well?22:12
yashgarothdepends heavily on how capable the robot is22:12
kanzurelet's say both, separate, and then just one or the other22:12
yashgarothhow about you make a robot that runs ELISAs, those are bitch work22:13
n_benthadon't they already have robots for that?22:14
kanzureno they are all custom built or cost too much22:14
kanzureby "cost" i mean "sold at a ridiculously high price"22:14
yashgarothfor reading and washing plates, but I dunno about the whole thing22:14
yashgarothlike I give it a hundred sample tubes, and the next morning it tells me the concentrations for each22:15
kanzureyes but again22:16
kanzurefor your company i think the value is something like salary per employee * at least 5 employees = X22:16
kanzurewell not just salary.. but also wasted materials, wasted time, overhead, ...22:17
yashgarothoh we probably pay a quarter mil per year for lab assistants22:17
n_benthathe ELISA_Starlet from hamilton robotics is what u want22:17
kanzurecompany of 30-50?22:17
yashgaroth24ish22:18
yashgarothand we're top-heavy22:18
kanzuren_bentha: yes but does it cost <$5k?22:18
n_benthain your dreams!22:19
kanzuren_bentha: how much would you pay for it?22:19
yashgarothooh or a cheap colony picking machine, we're still paying phd's to pick colonies because the machine breaks22:19
kanzurehow much do you /want/ to pay for it, i mean22:19
kanzurecome on.. use numbers22:19
n_benthai'd pay 0 dollars becuase I don't have the money. If I had a large facility that I was running (hospital?), then I'd pay alot for it.22:20
kanzurewrong answer22:20
fennwrong question22:20
fenn0 is the correct answer22:20
fennanyway, what i'm after is the maximum your PI would be willing to pay22:20
kanzurehaha that's not how PIs work though22:20
kanzurethey just write the cost into the grant22:20
yashgarothif you can entirely replace a person, then it's easily worth their salary, especially if the person you replace was annoying22:20
kanzurefenn: i think biotech companies are a better vector22:21
n_benthake ke. Idk. I'd say 50k would be reasonable if the accuracy is high.22:21
kanzureesp. for cost questios22:21
fennbiotech companies can already just buy an expensive robot22:21
yashgarothehh hospitals do a lot more repetitive shit, and they have just as much money22:21
kanzurefenn: then make an expensive robot22:21
fennok so why aren't they using robots?22:21
fenni'll cover it in gold plating if you want22:22
kanzureif $5k will get something that is high precision,22:22
kanzurethen $200k should be talking to my fucking car parked down the street22:22
fennwtf do you need precision for in a lab robot22:22
n_benthahaha, funny fenn.22:22
yashgarothheh22:22
fennat most it needs to be repeatable to within a 1mm22:22
n_bentha1mm is distance, i'm talking about results22:22
fennnow i'm confused. are you talking about volume?22:23
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n_benthanevermind fenn.22:24
* fenn mumbles something about market research firms22:24
n_bentha(accuracy of true values vs values the robot gives from doing the assays)22:25
kanzuren_bentha: please rant about your dream features :P22:25
kanzurealso! would you like a dna synthesizer with that?22:26
n_benthaOnly if it's an enzymatic dna synthesizer ;)22:26
kanzurewhat about one that works based on ligation?22:26
kanzurelike a library-based ligating device22:26
fennok i see, you're talking about something that does ELISA and i'm talking about something that just moves around and pipets stuff from one well to another22:27
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n_benthaah ok fenn. but ya you can do better than 1mm accuracy even w/ moving things around.22:27
n_benthasome robots can be calibrated to be very precise22:28
fennsure, 0.1mm is easy22:28
n_benthakanzure: wouldn't a dna synth like that need a large library and lots of up-keep? or could it use enzymes and primers to re-generate its library?22:30
n_benthaman, we had this one robot....it was such a pain. it took so long to calibrate, and wouldn't work as fast as we had hoped, and then it didn't do it's job properly for a while at the beggining.22:31
n_benthaI'm still faster and more efficient than it is...but it's nice cuz it reduces eye and hand strain and is consistent now that it's 'optimized'.22:32
fennwhat do you mean calibrating?22:33
n_benthawell u have to teach the robot where things are each time, and adjust parameters to make it work well22:34
fennbecause plates are different?22:35
fenni can't really see selling a robot that didnt work out of the box22:35
n_benthaIt depends on how the robot is made and works.22:35
fennlet's say it has a table with a grid of attachment points22:36
fennyou open up the software and drag an icon of a plate carrier to the grid representation onscreen22:37
n_benthaBut when you have a sample somewhere in space, and the robot is working around it w/ high prescision. Then you need to tell the robot this is point (0, 0), this is point (0, 100) and this is point (100,0) so that it knows the spacial relations of things.22:37
katsmeowvision would be oodles of help, i think22:37
n_benthaIf the robot had some sensors or someshit, then I guess it wouldn't need a hoomin to adjust it, right?22:37
fennno sensors needed, except maybe limit switches22:37
n_benthaLike lasers or cameras?22:38
n_benthaIt's hard to make limit switches that small though....22:38
fenn(of course i'd add some vision to check that the liquid in your pipet doesn't have any bubbles)22:38
fennthe switches go on the robot's axes, inside the mechanism22:38
n_benthaEven w/ the robot, we had to make sure it wasn't taking up air.22:39
fennright22:39
n_benthabut what if the robot is not confined, but moving around an area freely22:39
fenni'm just confused why a manufacturer would require you to calibrate something obvious like the zero position22:39
n_benthathat's what i had to deal w/ :(22:39
n_benthaidk either. i thought it was stupid. i didn't pay for the thing.22:40
kanzurewho did?22:40
fennnames have been changed to protect the guilty22:40
yashgarothPI/taxpayers, presumably22:40
katsmeowbecause even bolting the root to the supposed zero position doesn't in fact make it be there22:40
fenneh, this is low precision stuff katsmeow22:41
fennno need for kinematic mounts or laser interferometry22:41
n_benthayes, PI + taxpayers22:41
yashgarotheven fitting a tip to a pipettor becomes high-precision without active tactile feedback22:42
n_benthawell i was dealing w/ high prescision stuff, fenn22:42
fennoh god nevermind22:42
n_benthabut you have to watch out for rotational velocidensity, you know22:42
fennjust grease the muffler bearings22:42
yashgarothnot to mention spatial incontrivances22:42
katsmeowlol22:42
* katsmeow has a space elevator question when this discussion is winding down22:43
fennspace elevators are dumb22:43
kanzurekatsmeow: just say it22:43
kanzurefuck22:43
fennget a rotating tether22:43
kanzureyou don't need permission22:43
fennwho dares ask questions without my permission!22:43
delinquentmeme like elevaters22:43
delinquentmejar!22:43
kanzuredelinquentme: you like anything22:43
fenngah LED lighting is weird22:44
delinquentmelolol22:44
katsmeowdidn't wanna interrupt, i was just grabbing a queue position22:44
delinquentmetrue22:44
katsmeowhow does the space elevator add to the rotaional velocity of an item it's dragging to space?22:45
kanzurefenn: have you been properly cultured lately? http://www.youtube.com/watch?v=iHJkyMo9MtU22:45
katsmeowthere's no diagonal bracing to keep the elevator from bending over22:45
fenncentripetal force22:45
delinquentmekatsmeow, cant you make it work w rockets?22:45
n_benthai thought we were just going to put a rail-gun type of launcher on mars?22:45
n_bentha(i meant the moon)22:45
yashgarothalso the massive counterweigh22:45
yashgarotht22:45
katsmeowwe aren't on Mars22:45
katsmeowwe aren't on the moon22:46
n_benthayea, but if u make the moon a space base, then it makes lots of sense22:46
katsmeowfenn, so the item going up will just accumulate power with no input just because it was raised?22:46
n_benthabut ya a space elevator might be helpful @ first22:46
fennkatsmeow: yep, because the earth is spinning at 1rev/day22:46
* katsmeow points to the ballerina spinning energy conservation scenario22:47
fennwe already have a velocity of about 1000mph just being on the surface22:47
yashgarothI'm no physitrist, but it takes the energy from slowing down the earth, no?22:47
katsmeowbut lifting something with no force means it is going to slow down relative to the surface22:47
n_benthawhich means that it's accelerating in the opposite direction!22:48
fennwho said "no force"? you're fighting against about 1g of downward acceleration most of the way up22:48
katsmeowballerina spins with arms out, brings arms in and speeds up very fast,, conservation of angular momentum,, how does the elevator not fall over?22:48
katsmeowfenn, "down" is the wrong vector22:48
fenndown is toward the center of the earth22:48
delinquentme^^22:49
fennforce is required to draw your arms in22:49
katsmeowyeas, nothing to do with the queston, other than you are moving away from it, because my question applies even if there was no planet22:49
fennthe most stable state has the highest moment of rotational inertia22:49
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n_benthadoesn't the most recently proposed space elevator concept use magnets to elevate the 'arm?'22:50
yashgarothit doesn't fall over because the counterweight, be it an asteroid or whatever, pulls it upright22:50
jrayhawkbahaha oh space elevator discussions22:51
katsmeowthat's a lot of tension pulling out to keep the item from pushing the elevator sideways22:51
yashgaroththat's why you build it out of Material X22:51
kanzureDNA2.0 Inc.'s stats: 30% pharma, 50% agricultural chem, 20% academia22:51
kanzure"They ship ~100 genes a day, avg gene size 1.7 kb."22:51
kanzure"Est they have 50% of us market"22:51
kanzure1/3 europe, 2/3 USA22:51
kanzure100 genes per day is 50% of the US market?22:52
kanzurewhat the fuck22:52
fennthere'd be higher demand if it didn't cost so much22:52
kanzureduh22:52
kanzure1.7 kb * $0.20/bp = aw hell no22:52
yashgarothwhere'd you get those stats from22:53
kanzureyashgaroth: someone went to a DNA 2.0 presentation for me22:53
kanzureso.. $340 * 100 = $34,000/day22:53
yashgarothp. sure that's only for codon-optimized genes, using their proprietary approach, not the whole dna synth market22:53
kanzuregah... idt said 25,000 orders per day22:54
fennkatsmeow: the tension force is because the cable weighs so much, not because it needs tension to keep from "falling over"22:54
fennit's already "falling" around the earth because it's in orbit22:54
kanzureyashgaroth: ah22:55
yashgarothyeah we tried them out, the codon optimization didn't help at all22:56
katsmeowAt the same time, if we are to send any kind of a payload up or down the space elevator, its angular momentum will change by two orders of magnitude: its angular velocity is the same at top as at bottom, but angular momentum is proportional to the square of radius, which varies by an order of magnitude in the transit: this will require the cable to apply a force to the payload at right angles to the direction of the cable.22:56
yashgaroththen again phage display panning already tends to select for codon-optimized genes22:57
n_benthaOMG OMG OMG22:57
n_benthaspace elevators are just like chromosome during anaphase!22:57
yashgarothwhat if we make the elevator out of dna and just ride a polymerase up it22:58
yashgarothor did I just blow your minds22:58
fennkatsmeow: the mass of the payload has to be some small fraction (10%?) of the counterweight22:58
kanzurewhat happened to just walking off the edge of the earth22:58
kanzurewith an extended pathway22:58
n_benthahehe, u need cpt. jack sparrow for that!22:59
fennkatsmeow: in many designs the mass of the cable itself compensates for a relatively small counterweight22:59
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katsmeowbut i am not concerned abot the counterweight, i am asking about the side forces,, and once the cable is pushed tot he side, the distances tween earth to cog and to the counterweight change23:00
fennthe cable is being pulled taut by its own weight and the counterweight23:01
katsmeowok23:01
fennyou are correct that the cable will bend23:01
fennbut the sideways force increases with the sine of the angle of the bend23:02
katsmeowwell, if they ask me about this, i will haveto study this more23:02
fenniirc there's a mechanism in the counterweight to reel the cable in and out to compensate for this, i never really looked into it23:03
n_benthaDo you think using lasers to power the climber is best?23:03
n_benthaWould putting sats in space that have solar panels and then beam their solar energy to the climber work?23:03
fenni think using nuclear reactors is best23:03
fennbut nobody cares about my opinion23:03
katsmeowwhatever lowers the weight of the car is best23:03
katsmeowlasers may damage the cable23:03
n_bentha:O23:04
fennit's a long way up23:04
fennhow do you even focus through all that soup23:04
n_benthaidk :(23:04
n_benthabut they did it in that movie...23:04
katsmeowright, diffusion and the cable wandering about23:04
fennkatsmeow: who are you presenting to?23:04
n_benthareal genius23:04
fennhehehe23:04
katsmeowno one23:04
fennok then i suggest reading the technical papers on tethers.com23:05
* katsmeow notes that23:05
fennhttp://tethers.com/Bibliography.html and http://tethers.com/MXTethers.html23:05
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katsmeowbefore i foget, speaking of robots, in a different topic, http://spectrum.ieee.org/automaton/robotics/humanoids/dlr-super-robust-robot-hand23:06
* katsmeow looks and feels tired23:06
kanzurefenn: did you see that thing about venus project transitioning to open source? or something23:07
kanzureposted to OM asking for help23:07
katsmeowfor along time i thought venus was part of seasteading23:07
kanzurevenus is just some grumpy old man's art project23:08
kanzureas far as i can tell.23:08
katsmeowk23:08
fenni think this is the right video about rotating tethers http://www.youtube.com/watch?v=mPx1Nq80jm823:08
fennpardon the overly epic music23:09
katsmeowno sound here23:09
fennat about 3:15 you can see the actual momentum exchange23:10
n_benthaFARIL23:10
n_benthaFAIL*23:10
n_benthaspace debris will wipe that shizz out23:10
n_benthastep 1: clean your work space23:11
* n_bentha goes to dream about robots and space elevators23:11
fennwhat's not clear in the video is that the yellow orbit line changes also23:11
fennn_bentha: hoyt has written extensively on the topic of space debris and creating collision resistant designs23:12
fennunfortunately the space elevator fanboys are ignorant of his work23:12
n_benthaya but u still need to clear up the debris for whatever you're going to be sending up into space23:13
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katsmeowhttp://www.youtube.com/watch?v=mPx1Nq80jm8  isn't about a space elevator, it's abot flinging stuff already in leo out away from earth23:15
fennthe concept generalizes to suborbital payloads23:15
fennit can pick thing up off the surface of the moon or from the stratosphere (supersonic aircraft)23:16
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fennthe tip velocity is too high to go deep into earth atmosphere; basically it will melt from friction23:17
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superkuhn_bentha, rotating electrodynamic tethers are the best solution for cleaning up orbit.23:23
superkuhhttp://www.youtube.com/watch?v=Pwro-ijCYZc23:24
superkuhhttp://erewhon.superkuh.com/library/Space/Spacecraft/EDDE_%20Electrodynamic%20Debris%20Eliminator%20for%20Active%20Debris%20Removal_%20conference_presentation.pdf23:25
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