2012-08-21.log

--- Log opened Tue Aug 21 00:00:00 2012
nmz787http://wholebraincatalog.org/00:05
-!- HariSeldon [~Shehrazad@88.243.141.196] has joined ##hplusroadmap00:05
-!- Guest19125 [~quassel@64.31.59.70] has quit [Read error: Operation timed out]00:06
-!- Charlie [~quassel@64.31.59.70] has joined ##hplusroadmap00:06
-!- Charlie is now known as Guest5679800:07
-!- Haruspex [~Shehrazad@88.243.141.196] has quit [Ping timeout: 240 seconds]00:08
nmz787this video is quite good http://www.cwgp.org/drew_berry.php00:08
-!- rmcl [~rmcl@99-71-136-35.lightspeed.sndgca.sbcglobal.net] has quit [Quit: rmcl]00:10
-!- sylph_mako [~mako@213.195.252.27.dyn.cust.vf.net.nz] has joined ##hplusroadmap00:16
-!- SolG [~Sol@c-174-57-58-11.hsd1.pa.comcast.net] has joined ##hplusroadmap00:31
-!- _sol_ [~Sol@c-174-57-58-11.hsd1.pa.comcast.net] has quit [Ping timeout: 245 seconds]00:32
-!- cutbert [~nima@2001:4830:1100:104::2] has quit [Ping timeout: 246 seconds]00:33
-!- cutbert [~nima@cl-261.bos-01.us.sixxs.net] has joined ##hplusroadmap00:33
-!- cutbert [~nima@cl-261.bos-01.us.sixxs.net] has quit [Ping timeout: 250 seconds]00:44
-!- cutbert [~nima@cl-261.bos-01.us.sixxs.net] has joined ##hplusroadmap00:44
-!- ThomasEgi [~thomas@p5B13B6DE.dip.t-dialin.net] has joined ##hplusroadmap01:14
-!- ThomasEgi [~thomas@p5B13B6DE.dip.t-dialin.net] has quit [Changing host]01:14
-!- ThomasEgi [~thomas@panda3d/ThomasEgi] has joined ##hplusroadmap01:14
-!- tashoutang [~tata@pc131090206.ntunhs.edu.tw] has quit []01:15
-!- tashoutang [~tata@pc131090206.ntunhs.edu.tw] has joined ##hplusroadmap01:23
-!- fenn_ is now known as fenn01:26
fenni'm a big fan of drew berry's work. downloaded all his stuff, it's around here somewhere..01:27
-!- qnm [~qnm@2001:44b8:3110:f300:208:9bff:fec0:179a] has quit [Read error: Connection refused]01:31
-!- qnm [~qnm@2001:44b8:3110:f300:208:9bff:fec0:179a] has joined ##hplusroadmap01:33
fenn"a day made of glass" is more about touchscreens and gestural interfaces than anything01:36
fennand, it makes me want to puke. EOM01:36
fenn"3d projection"? wtf is that?01:37
fennwhy are there humans driving the cars?01:38
fennwhy do the kids have backpacks when they have these super fancy smartphones?01:39
-!- nmz787 [~Nathan@ool-457e5cbd.dyn.optonline.net] has quit [Quit: Leaving.]01:40
strangewarpThe humans are driving cars because of shitty moral-panic-driven laws against driverless cars01:44
strangewarpThe kids have backpacks for bottles of alcohol01:44
strangewarpgosh it's obvious01:44
fennthis video would make a lot more sense if it had been made by samsung01:46
fennthey're already making those see through window displays01:46
fenn"driverless cars" reminds me of "horseless carriage"01:47
fennif the tablets werent made of glass they wouldnt need cases01:47
fennand a glass dashboard seems like a terrible idea01:48
-!- Mokbortolan_1 [~Nate@c-76-115-136-13.hsd1.or.comcast.net] has joined ##hplusroadmap01:53
-!- Mokbortolan_ [~Nate@c-76-115-136-13.hsd1.or.comcast.net] has quit [Ping timeout: 246 seconds]01:55
-!- nmz787 [~Nathan@ool-457e5cbd.dyn.optonline.net] has joined ##hplusroadmap01:59
nmz787fenn, so I heard microstepping is bad if you use a motor that's designed for high holding torque02:00
nmz787because the magnetic field doesn't fall off from the tooth with the cosine/sine rule02:00
fennyes, i told you about that a long time ago02:02
-!- archels [~foo@sascha.esrac.ele.tue.nl] has quit [Ping timeout: 244 seconds]02:02
fenni know the guy who designed the lookup table to compensate for that02:03
fennsame lookup table is used in almost every stepper controller chip, but it's not perfect02:03
nmz787this guy is where i first saw it http://users.ece.utexas.edu/~valvano/Datasheets/StepperMicrostep.pdf02:04
nmz787he has a lot of other papers too if you just go up the URL02:04
nmz787http://users.ece.utexas.edu/~valvano/Datasheets/StepperSelection.pdf02:04
nmz787well this was saying that you just shouldn't use high-holding torque motors02:04
nmz787if you want good microstepping02:05
-!- delinquentme [~asdfasdf@c-71-236-101-39.hsd1.pa.comcast.net] has quit [Ping timeout: 276 seconds]02:05
fennso you want something like slo-syn instead?02:05
fennactually i don't know which kind of motors are most linear02:06
nmz787what's that?02:06
fennan old motor design you find in a lot of scrapped scientific equipment, it's usually painted grayish teal, and looks like a fat cylinder02:06
nmz787hmm http://www.contraptor.org/forum/t-273788/micro-cnc-laser02:07
fennthey can be driven by sinusoidal AC or using square waves02:07
fennheh contraptor, i forgot about that guy02:07
nmz787so why didn't you choose to build the laser_etcher to look like this? http://cncestablishment.com/wp-content/uploads/2011/11/cnc-engraving-machine.jpg02:08
fenni explained all that in the last email02:09
fenni could be wrong. only one way to find out02:09
nmz787hmm02:10
fennthe contraptor forum etcher looks pretty good02:10
nmz787i don't really see an explanation of this particular config02:10
nmz787you talked about MDF02:10
nmz787and oil bearings02:10
fennokay what is the exact question then?02:10
nmz787well that image that i just posted *looks* different than your SCAD rendering02:11
-!- tashoutang [~tata@pc131090206.ntunhs.edu.tw] has quit []02:11
fennin many ways02:11
-!- archels [~foo@sascha.esrac.ele.tue.nl] has joined ##hplusroadmap02:12
fennit's not a rendering, for one02:12
nmz787uhh02:12
nmz787a screenshot of a rendering?02:12
fennyou mean why do i have two sets of ways bolted to the same plate?02:13
fennor "why didnt you make a gantry router"02:13
nmz787well they both apply02:13
fenn(also those things suck as routers)02:13
-!- augur_ [~augur@208.58.5.87] has joined ##hplusroadmap02:13
nmz787yeah so both of those questions02:13
fenn1) for rigidity, 2) i thought it would be better to keep the laser tube stationary if we decided to use a CO2 laser02:14
-!- augur [~augur@208.58.5.87] has quit [Read error: Connection reset by peer]02:15
fennadding flying optics would complicate the design02:15
nmz787sure02:15
nmz787so is that model complete?02:16
fennno02:16
nmz787if I got openSCAD running could I follow it as a pictorial guide to getting it together?02:16
fennyes02:16
fennyou'd have to calculate some dimensions though02:16
fennas far as i can tell there are no "measure this distance" tools in openscad02:16
nmz787so what's missing, if its not completer02:16
nmz787complete*02:16
nmz787or is it those exact distances that is missing?02:17
fennit's missing the top and bottom plates, the bolt hole locations, the leadscrews and leadnuts and bearing mounts and stepper motor mounts, and it needs some kind of right angle bracket to hold the laser optics02:17
nmz787did you just eyeball the structure with no real measurments?02:17
fennthe structure is eyeballed, but the parts are modeled after real parts (or the provided cad drawings at least)02:18
fennit should look like the rendering if built to the dimensions in the openscad file02:18
nmz787for the optics you mean anything that could hover about the setup?02:18
fennright02:18
fenni was thinking some kind of optical breadboard at 90 degrees to the motion stage02:19
nmz787hmm02:19
nmz787the screws, bearings and motor mounts are the only things I think I am not clear on then02:19
nmz787rather, how those items connect together02:20
fennhm, i hadn't tested to make sure all the dimensions work the way i had imagined02:23
fennbut it should be close enough to take out any vertical slop with shims02:23
nmz787hmm, any chance you'd be interested in adding the screws and bearings and motor mounts to the CAD ?02:24
fenndo you have the file support_block.scad in your working dir?02:25
fennjust wondering if i had pushed it earlier02:25
nmz787its here http://diyhpl.us/laser_etcher/02:28
fennok02:28
-!- tashoutang [~tata@pc131090206.ntunhs.edu.tw] has joined ##hplusroadmap02:31
fennyou should add links to any whitepapers you find relevant in the laser_etcher.mdwn02:31
nmz787ahh, ok02:32
fenni was considering using a "flexure bearing" to connect the lead nut and the moving part of each axis02:32
fennbut it turns out one can't simply buy his way into mordor02:33
fenni mean, buy a flexure bearing02:33
nmz787hmm02:35
fennthe point would be to prevent rocking deflection perpendicular to the axis of motion from a slightly eccentric leadscrew02:35
fennthis was a problem on the makerbot Z axis at one point and solved in a similar way02:36
nmz787so is it just a bearing in a rubber block?02:36
fennno02:36
fennit only flexes in one direction, or two directions02:37
fennone moment, the internet is not cooperating02:37
-!- tashoutang [~tata@pc131090206.ntunhs.edu.tw] has quit []02:38
-!- ThomasEgi [~thomas@panda3d/ThomasEgi] has quit [Remote host closed the connection]02:39
fennblarg. well this is the best pic of it i can find: http://www.thingiverse.com/thing:2151 http://www.thingiverse.com/image:3671302:43
fennit's not a particularly brilliant design but i hope you get the idea02:43
fennthe vertical rigidity is greater than the horizontal rigidity02:43
fennso the nut can move around in X and Y without affecting the Z position02:44
fennoh, the makerbot wobble arrester is only really designed to flex in one direction02:46
nmz787hmm, so you can't buy one of those?02:48
fennnot that i can find02:49
fennit's a trivial waterjet job though02:49
nmz787can we just use that design, printed locally or from shapeways?02:49
nmz787or is that design inadequate?02:50
fennit would need to be modified to fit our nut, and then might as well just redesign it from scratch02:50
fennit may not be necessary at all though02:51
nmz787seems like its just a spring though, which on the makerbot Z aren't there multiple screws?02:51
fenni just wanted to convey the concept to you02:51
nmz787where we'll only have 1 screw in any direction02:51
fennthese are true statements, but i don't see your point02:52
nmz787so on the makerbot it seems like the actual position of the stage would just be something in between all 4 Z screws, thanks to the wobble arrester springs02:52
fennoh. yeah, the makerbot is overconstrained02:52
nmz787well I guess we'd need this item on the nut, and leave the Z constrained by the linear rails?02:53
fennyes02:53
nmz787without this, it would just put undue pressure on the plate/slide bearings02:53
nmz787i think02:54
fennthis is to prevent an eccentric leadscrew from causing uneven friction with the nut, and to prevent side loading of the axis02:54
nmz787right02:54
nmz787ok02:54
fennso i remember where i stopped working last time, i was trying to "save time" by using existing bearing and stepper motor models02:55
fennbut the existing libraries are more of a pain to use than they're worth02:55
nmz787I remember you linked to something like 'worlds smallest Z stepper' or something a while ago02:58
nmz787something from a CD drive I think02:58
nmz787hacked02:59
gnushalaser_etcher.git: 7d48ccc 03:00
gnushalaser_etcher.git: 8548e40 03:01
fennwas there supposed to be a commit message?03:06
fennhm i guess not03:07
gnushalaser_etcher.git: c8f5071 03:07
-!- sylph_mako [~mako@213.195.252.27.dyn.cust.vf.net.nz] has quit [Quit: Leaving]03:08
-!- qnm [~qnm@2001:44b8:3110:f300:208:9bff:fec0:179a] has quit [Ping timeout: 240 seconds]03:11
-!- qnm [~qnm@2001:44b8:3110:f300:208:9bff:fec0:179a] has joined ##hplusroadmap03:13
nmz787fenn, anything that I could help with to further your completion of that model?03:16
fennfind me a nema23 stepper model in openscad03:18
fennand a 16mm bearing model and 20mm ish thrust bearing03:19
nmz787ok, will try to do when i wake up03:19
* nmz787 heads toward bed03:19
fennperhaps i can just use simple cylinders as stand ins03:19
fenngnight03:19
-!- nmz787 [~Nathan@ool-457e5cbd.dyn.optonline.net] has quit [Quit: Leaving.]03:20
-!- delinquentme [~asdfasdf@c-71-236-101-39.hsd1.pa.comcast.net] has joined ##hplusroadmap03:42
-!- delinquentme [~asdfasdf@c-71-236-101-39.hsd1.pa.comcast.net] has quit [Ping timeout: 276 seconds]04:06
-!- chris_99 [~chris_99@unaffiliated/chris-99/x-3062929] has joined ##hplusroadmap04:14
-!- AdrienG [~ircname@unaffiliated/amphetamine] has joined ##hplusroadmap04:20
-!- AdrienG is now known as AdrianG04:20
-!- EnLilaSko [~Nattzor@unaffiliated/enlilasko] has joined ##hplusroadmap04:24
-!- chris_99 [~chris_99@unaffiliated/chris-99/x-3062929] has quit [Ping timeout: 276 seconds]04:30
@kanzureoh look. a wild fenn appeared!04:32
foucistquick, get the pokeball out!04:34
@kanzurei am a conscientious objector04:43
-!- chris_99 [~chris_99@unaffiliated/chris-99/x-3062929] has joined ##hplusroadmap04:43
gnushalaser_etcher.git: f928f89 added motor and bracket mockup, need accurate dimensions still04:54
gnushalaser_etcher.git: e56df72 clean up coupler dimensions, add hole to bracket for motor shaft04:54
gnushalaser_etcher.git: b196e42 added some leadscrew jazz04:54
gnushalaser_etcher.git: 461f9c6 finish leadscrew04:54
fennlet nmz787 know about  http://fennetic.net/irc/leadscrew_assembly.png04:55
@kanzurehow do i convince him to use commit messages?04:55
fennwith a large blunt object04:56
fennsuch as a trout04:56
@kanzure... or maybe a pokeball?04:56
fennnot large enough04:56
fennis there a way to measure distances between points in an stl file?04:57
@kanzurestl is dimensionless but i think blender has some things that pretend to be rulers and measuring sticks?04:58
fennlike, click here, click here, what's the distance04:58
fennoh right, blender04:58
fennthat nema 23 motor looks really huge in comparison04:59
@kanzurewhich scad nema motors did you look at?05:02
fennsome stuff in MCAD05:03
@kanzurethere's also things like.. http://www.thingiverse.com/thing:316505:04
@kanzureoh nevermind.. elmom considers his version more complete05:05
fenncube(size=[bolt_hole_distance+bolt_hole_size+5,05:05
fennreally? the cube is just 5 mm bigger than the bolt holes?05:05
fennwell i guess that works05:06
fennnema spec says no greater than 57mm05:06
fenn1.856in+0.195in+5mm = 57.09 mm05:07
fennblarg why can't everyone just use mm05:08
fennthey also seem to think the motor shaft is 6.36mm while the motors i have are actually 5mm05:10
fennmaybe i should measure that again05:10
fennso, i'm probably going to go be a hippie and build earthbag domes in the desert05:12
@kanzureis this just a creative way of saying "i'm going to burning man again"?05:13
fennnot that i'm particularly fond of digging in the dirt, but it seems to be a proven design05:13
fenni'm not going to burning man this year05:13
fenns/earthbag/superadobe/05:14
fennideally i'd have a robocrane squirt out a concrete shell just like a giant reprap05:15
fenndid dave take my electronics junk too? or is that still in austin?05:17
@kanzureles might still have a few boxes05:18
@kanzurei don't think i had any05:18
fennyou have some sensors from sparkfun and a lego house05:18
fennand possibly some documents05:18
@kanzuresounds right to me05:18
fenni should tell les about magnesium05:19
fenni've never seen the man touch a green piece of food05:19
@kanzurei think his diet is taco bell sauce packets05:20
fennand whataburger shakes05:20
fennmy new breakfast: 1c hot water, 1T miso, 1t dried seaweed, 1t kelp powder, 1c soba, 1/2 can salmon05:21
fennit's practically instant if you have a zojirushi05:22
fenn(hot water dispenser)05:22
fennsubstitute mackerel for salmon if you're poor05:24
gnushalaser_etcher.git: 67bbbbf i guess i have to use colors inside the module05:29
fennah good push works as expected now05:29
@kanzurelooks the same?05:30
fenn[remote "origin"]05:32
fenn        url = fenn@diyhpl.us:/srv/git/laser_etcher.git05:32
fenn works now with "git push"05:32
fennbefore i had to do git push fenn@diyhpl.us:/srv/git/laser_etcher.git master05:32
@kanzureoh i assumed you had that05:32
@kanzurei see05:32
fenni did have that05:32
fennbut something changed and it works now.. anyway05:32
-!- devrandom [~devrandom@gateway/tor-sasl/niftyzero1] has quit [Write error: Broken pipe]05:45
-!- ParahSailin_ [~parah@69.151.157.115] has joined ##hplusroadmap05:47
-!- devrandom [~devrandom@gateway/tor-sasl/niftyzero1] has joined ##hplusroadmap05:52
-!- nsh [~Smedley@wikipedia/nsh] has quit [Ping timeout: 248 seconds]06:00
-!- nsh [~Smedley@host86-137-188-83.range86-137.btcentralplus.com] has joined ##hplusroadmap06:45
-!- nsh [~Smedley@host86-137-188-83.range86-137.btcentralplus.com] has quit [Changing host]06:45
-!- nsh [~Smedley@wikipedia/nsh] has joined ##hplusroadmap06:45
-!- OldCoder_ [~OldCoder_@c-69-181-140-134.hsd1.ca.comcast.net] has quit [Quit: Leaving]06:51
-!- OldCoder_ [~OldCoder_@c-69-181-140-134.hsd1.ca.comcast.net] has joined ##hplusroadmap06:53
-!- minimoose [~minimoose@209.140.69.189] has joined ##hplusroadmap07:02
-!- minimoose [~minimoose@209.140.69.189] has quit [Read error: Connection reset by peer]07:17
@kanzure"The research you've published in Nucleic Acids Research has contributed to the journal's highest-ever 8.026 Impact Factor from Thomson Reuters' Journal Citation Reports."07:52
-!- qnm [~qnm@2001:44b8:3110:f300:208:9bff:fec0:179a] has quit [Ping timeout: 260 seconds]08:03
-!- qnm [~qnm@2001:44b8:3110:f300:208:9bff:fec0:179a] has joined ##hplusroadmap08:06
@kanzurehmm "Astrobotic today announced a NASA contract to develop technologies for exploring caves on the Moon" so uh, what caves are there?08:12
chris_99can you get high resolution servo controlled robotic arms?08:19
jrayhawk[url "jrayhawk@diyhpl.us:/srv/git/"] pushInsteadOf = git://diyhpl.us/08:29
jrayhawk        pushInsteadOf = git://diyhpl.us/08:29
jrayhawkugh, i wonder why that doesn't copy and paste right08:29
jrayhawk[url "jrayhawk@diyhpl.us:/srv/git/"]08:30
jrayhawk        pushInsteadOf = git://diyhpl.us/08:30
jrayhawkthere we go08:30
jrayhawkdoing something like that might also make your lives slightly easier08:30
@kanzurei never try to push via git:// on diyhpl.us08:33
jrayhawkthat would make sense considering it wouldn't work without some special effort08:34
@kanzureis "special effort" a euphemism for "jrayhawk does it"08:35
jrayhawkpossibly. i don't remember if git-daemon needs to have its command line changed, but at least the repositories need a special configuration to allow that sort of export.08:36
@kanzurei see08:36
@kanzure(it's not particularly important to me)08:36
jrayhawkcore.sharedrepository needs to be 0666, obviously08:36
jrayhawkdaemon.uploadarch needs to be set to true, i guess08:37
jrayhawkand daemon.receivepack08:38
jrayhawkanyway, once you have that pushInsteadOf override in place, you can switch remote.origin.url over to git://08:39
jrayhawkand pulling is magically less irritating08:39
-!- augur_ [~augur@208.58.5.87] has quit [Remote host closed the connection]09:07
-!- Falfe [~not@c83-251-81-162.bredband.comhem.se] has joined ##hplusroadmap09:09
@kanzure"As far as I can tell, biohackers are all adventurous young people, incredibly athletic, and theyā€™re all traveling the world."09:10
@kanzurestewart brand has a messed up conception of me :P09:10
@kanzurehttp://www.wired.com/wiredenterprise/2012/08/ff_stewartbrand/all/09:10
@kanzurebut i do find it funny that kevin kelly and stew are still pimping well09:11
@kanzurei mean, Well09:11
-!- ThomasEgi [~thomas@gw-ko-kostr.inka-online.net] has joined ##hplusroadmap09:22
-!- ThomasEgi [~thomas@gw-ko-kostr.inka-online.net] has quit [Changing host]09:22
-!- ThomasEgi [~thomas@panda3d/ThomasEgi] has joined ##hplusroadmap09:22
-!- jmil [~jmil@hive76/member/jmil] has joined ##hplusroadmap09:41
-!- augur [~augur@129-2-129-33.wireless.umd.edu] has joined ##hplusroadmap09:52
-!- rmcl [~rmcl@75.36.45.199] has joined ##hplusroadmap10:06
-!- rmcl [~rmcl@75.36.45.199] has quit [Quit: rmcl]10:17
archelsseems the media is all over everyone recently who is affiliated with the Long Now Foundation.10:18
@kanzurenah.. stew/kk/that gang has had positive media since forever10:25
@kanzureeveryone likes the "friendly old guys talking about edge.org stuff"10:25
@kanzure"edge.org: TED but without herpes"10:25
@kanzureoh he did venter recently.. http://edge.org/conversation/what-is-life10:27
@kanzure(video)10:27
@kanzureoh nope. there's a transcript.10:27
-!- octopine is now known as audy10:58
-!- mensch [~mensch@c-75-69-228-244.hsd1.ma.comcast.net] has joined ##hplusroadmap11:39
-!- jmil [~jmil@hive76/member/jmil] has quit [Quit: jmil]11:43
archelsBy agreement with the publisher, this book is accessible by the search feature, but cannot be browsed.11:49
archels^ on pubmed. Interesting.11:49
-!- nsh [~Smedley@wikipedia/nsh] has quit [Read error: Connection timed out]11:50
@kanzurearchels: pubmed is negotiating with publishers?11:50
-!- nsh [~Smedley@host86-137-188-83.range86-137.btcentralplus.com] has joined ##hplusroadmap11:51
-!- eridu [~eridu@gateway/tor-sasl/eridu] has joined ##hplusroadmap11:51
-!- eridu [~eridu@gateway/tor-sasl/eridu] has quit [Write error: Broken pipe]11:57
-!- eridu [~eridu@gateway/tor-sasl/eridu] has joined ##hplusroadmap12:00
-!- eridu [~eridu@gateway/tor-sasl/eridu] has quit [Client Quit]12:03
-!- Mokbortolan_ [~Nate@c-76-115-136-13.hsd1.or.comcast.net] has joined ##hplusroadmap12:19
-!- Mokbortolan_1 [~Nate@c-76-115-136-13.hsd1.or.comcast.net] has quit [Ping timeout: 260 seconds]12:21
-!- AdrienG [~ircname@unaffiliated/amphetamine] has joined ##hplusroadmap12:22
-!- AdrianG [~ircname@unaffiliated/amphetamine] has quit [Ping timeout: 240 seconds]12:26
-!- eridu [~eridu@gateway/tor-sasl/eridu] has joined ##hplusroadmap12:31
-!- nmz787 [~Nathan@173-114-252-246.pools.spcsdns.net] has joined ##hplusroadmap12:52
-!- Jaakko96 [~Jaakko@94-194-89-130.zone8.bethere.co.uk] has joined ##hplusroadmap12:56
@kanzurenmz787: how's meredith?12:57
-!- eridu [~eridu@gateway/tor-sasl/eridu] has quit [Ping timeout: 276 seconds]12:57
-!- eridu [~eridu@gateway/tor-sasl/eridu] has joined ##hplusroadmap13:05
-!- drazak__ [~ahdfadkfa@69.162.134.185] has joined ##hplusroadmap13:10
-!- drazak__ [~ahdfadkfa@69.162.134.185] has quit [Client Quit]13:10
-!- Jaakko96 [~Jaakko@94-194-89-130.zone8.bethere.co.uk] has quit [Quit: Nettalk6 - www.ntalk.de]13:15
-!- eridu [~eridu@gateway/tor-sasl/eridu] has quit [Ping timeout: 276 seconds]13:16
-!- marainein [~net@2001:388:608c:6cb5:d06:5bd6:a36f:c92] has quit [Ping timeout: 245 seconds]13:17
-!- nmz787 [~Nathan@173-114-252-246.pools.spcsdns.net] has quit [Quit: Leaving.]13:19
-!- marainein [~net@2001:388:608c:6cb5:c85a:fbcc:e87f:e157] has joined ##hplusroadmap13:30
-!- Mokbortolan_1 [~Nate@c-76-115-136-13.hsd1.or.comcast.net] has joined ##hplusroadmap13:32
-!- Mokbortolan_ [~Nate@c-76-115-136-13.hsd1.or.comcast.net] has quit [Ping timeout: 244 seconds]13:33
-!- strangewarp [~strangewa@c-76-25-200-47.hsd1.co.comcast.net] has quit [Ping timeout: 244 seconds]13:38
-!- strangewarp [~strangewa@c-76-25-200-47.hsd1.co.comcast.net] has joined ##hplusroadmap13:40
@kanzurehttp://options-vs-salary.com/ is a neat tool.13:41
chris_99fancy13:44
browniesnot a very good UX13:51
browniesbut i guess it's nice of them to do the multiplication for you13:51
-!- AdrienG [~ircname@unaffiliated/amphetamine] has left ##hplusroadmap []13:52
-!- yashgaroth [~f@cpe-66-27-117-179.san.res.rr.com] has joined ##hplusroadmap14:01
-!- Juul [~Juul@208.87.217.74] has joined ##hplusroadmap14:15
chris_99http://neurophone.wordpress.com/2012/08/05/make-a-diy-flanagan-neurophone-with-a-tl494/#14:18
-!- loanshark [~loanshark@ip72-218-133-8.hr.hr.cox.net] has joined ##hplusroadmap14:22
@kanzurehi loanshark14:22
loansharkhey again14:23
@kanzureso you want to get into bioengineering14:23
@kanzuretry this? http://diyhpl.us/wiki/diybio/faq14:23
loansharkyes!14:23
loansharkthankyou14:23
loansharki have one question14:23
loansharkits about obtaining DNA because i watched that video about making antidepressant yogurt14:24
@kanzurethat guy didn't really make antidepressant yogurt14:24
@kanzurehe was bullshitting you14:24
loansharkwhere do you order the genes?14:24
loansharko.o14:24
loansharkawww14:24
yashgaroththere's no prozac gene14:24
loansharki thought that was cool14:24
@kanzureas far as i know there's no prozac gene or prozac metabolic pathway14:25
yashgarothplus it's got a bunch of fluorines on there which is pretty much impossible to do enzymatically14:25
EnLilaSkoloanshark: The video was pretty much showing you can do shittons of stuff at home, but the prozac part was just a joke/troll14:26
yashgarothI wish he14:26
yashgaroth'd made that last part more obvious14:26
loansharkxD14:26
@kanzureyashgaroth: i should just make wild claims like that too14:26
@kanzureyashgaroth: "i have created yogurt that turns me into a hermit!"14:27
@kanzurewait maybe that's not sufficiently impressive14:27
EnLilaSkolol14:27
loanshark:314:27
yashgarothoh I made a talking mouse14:27
-!- Juul [~Juul@208.87.217.74] has quit [Ping timeout: 260 seconds]14:27
loansharkanyways, where do you get the DNA?14:27
loansharko.o14:27
EnLilaSko"I make a yogurt curing AIDS"14:27
EnLilaSkoAll the newspaper would write about it14:27
yashgarothwhat DNA14:27
EnLilaSkoHe gives you a site loanshark.14:27
@kanzureloanshark: you can get dna from yourself14:27
EnLilaSkoThe bacterias14:27
yashgarothI poured this yogurt into a dish of cancer cells and they all died, money please14:28
yashgarothwhy is no one funding this?!?!14:28
loansharkEnLilaSko: i still don't see how to actually get the DNA, there is no "buy now" xD14:28
ParahSailini made yogurt that was an AGI, but i got too hungry and ate it14:28
@kanzureloanshark: there is "buy now", you are lying14:28
EnLilaSkoAh, then I have no clue14:28
yashgarothorigene.com14:28
loanshark:o14:28
@kanzureor idtdna.com is a popular choice14:28
EnLilaSkoI usually buy cooler people genes so I maybe become cool14:29
loansharkso14:29
loansharkits $22014:29
loansharkxD14:29
loansharkDIY bioengineering is expensive!14:29
yashgarothwhat gene are you looking at14:30
loansharkidk14:30
loanshark:314:30
* loanshark is a newb14:31
@kanzureidk isn't a really interesting gene14:31
@kanzurewhy are you looking at idk14:31
loansharko.o14:31
yashgarothI saw a paper about idk3 recently, has a novel protease function14:31
loansharkok14:32
loansharkI'm gonna read that think kanzure gave me14:32
loansharkbecause i know zilch about genes and stuff14:32
@kanzurealso read everything here http://diyhpl.us/~bryan/papers2/bio/14:32
@kanzureand http://diyhpl.us/wiki/diybio/faq14:32
loanshark;_;14:32
loansharkcan i just buy a book14:32
loansharkwith everything in it14:33
yashgarothI recommend molecular biology of the cell, or molecular biology of the gene14:33
@kanzureno14:33
loansharklike my big encyclopedia of sailing14:33
yashgarothbut they don't really cover actual genetic engineering14:33
loansharkwhat have you guys done with genes?14:33
@kanzurealso, that question is answered in the faq14:33
@kanzureso is that one14:33
yashgarothbro I do stuff with genes erry day14:34
loansharkso many windows open now my head hurts x_x14:34
EnLilaSkoI like, live with mine14:34
yashgaroththough you should know, people have spent more hours writing news articles about biohacking than actually biohacking14:35
@kanzureloanshark: you can never have enough tabs http://heybryan.org/shots/2008-01-15-tabs.png14:35
@kanzurehttps://addons.mozilla.org/en-US/firefox/addon/toomanytabs-saves-your-memory/14:35
loansharkxD14:35
EnLilaSkodamn, lol14:36
loanshark:3 learning is fun14:40
loansharkare there any easy, cheap projects i can do to get started?14:40
@kanzurehttp://diyhpl.us/wiki/diybio/faq/kits14:41
yashgarothdefine easy/cheap14:41
loansharkumm14:41
loansharkunder $10014:41
@kanzureyou can do the shotglass dna extraction project14:41
@kanzurebut it's more of a parlor trick and completely useless14:41
@kanzureunless you happen to need to purify human dna for some project14:42
loansharkwhat do you do with the DNA that has been extracted?14:42
@kanzureyou eat it14:42
@kanzureor just stare at it14:42
TheEmpathom nom life books14:42
yashgarothyou get drunk in the process, that's a bonus14:42
loansharkyou can't do anything at all with it?14:42
@kanzureloanshark: biology is not cheap.. you shouldn't put price limits like that on it14:43
@kanzurethere's a $25 lightbulb pcr machine design that russell put up14:43
@kanzureand staceyk put up a $85 arduino-based pcr machine too, but you need to buy >$100 of pcr master mix (which you can sometimes get a free sample for..)14:43
yashgarothbut if you want to see the result of the pcr, you'll need a gelbox and supplies for that, oh and a centrifuge14:44
loansharkwhats a pcr?14:44
yashgarothhttp://www.ncbi.nlm.nih.gov/books/NBK21054/14:45
gnushadiyhpluswiki.git: 22b779a add a link to the kits page from diybio/faq.mdwn14:46
loansharkhttp://www.amazon.com/Biopunk-Scientists-Hack-Software-Life/dp/1617230022/ref=sr_1_1?ie=UTF8&qid=1345585572&sr=8-1&keywords=biohack14:46
loansharkmaybe?14:46
yashgarothdoesn't actually have any protocols, but sure14:46
yashgarothplease see [14:35:18] .:yashgaroth:. though you should know, people have spent more hours writing news articles about biohacking than actually biohacking14:46
loansharkakk14:46
loansharknvm14:46
gnushadiyhpluswiki.git: 0a48059 add molecular biology of the cell on nih to book list14:47
@kanzureloanshark: that book is just about me and my friends14:47
@kanzurei mean, it doesn't actually tell you how to do things14:47
yashgarothgenetic engineering isn't its own field, it's a very specialized application of biology14:47
loanshark>.<14:47
@kanzureloanshark: http://www.ncbi.nlm.nih.gov/books/NBK21054/ is a good intro book, but it wont teach you techniques14:48
@kanzureoh wait, it seems to have some chapters on "methods" in part 314:48
@kanzureyashgaroth: would you believe me if i told you i haven't actually read that book?14:48
yashgarothno, but it's questionable whether knowing how to run PCR is worthwhile if you don't know what PCR is14:48
yashgarothsure kanzure14:48
yashgarothbut you know what pcr is so it's a moot point14:49
loansharkoki14:49
loansharkgetting the 5th edition14:49
@kanzurehuh? why the 5th?14:49
@kanzureit's right here: http://www.ncbi.nlm.nih.gov/books/NBK21054/14:49
@kanzureboom you have it14:49
yashgarothnothing you'll need to know outside of a bio PhD program has been added between editions, but feel free14:50
@kanzureyashgaroth: maybe there's a cut-away phd hidden between pages 100 and page 10114:50
loansharki was just downloading it o.o14:50
yashgarothinclude 8 years of your life in the mail-away envelope14:50
loansharkits $159 dollars!14:51
@kanzureyes, because textbooks are evil14:51
@kanzurewhy are you paying for it?14:51
yashgarothoh we're the last people who'll tell you to pay for it14:51
loansharkim not14:51
loansharkbut still14:51
loansharkthats a lot14:51
@kanzureyashgaroth: oh that book isn't readable on the nih site14:52
yashgarothlolwut14:52
@kanzureas far as i can tell?14:52
yashgarothoh haha well fuck 'em then14:52
yashgarothyeah you're right, just noticed the header14:52
yashgaroth"By agreement with the publisher, this book is accessible by thepiratebay, but cannot be browsed."14:53
loanshark:314:53
-!- jmil [~jmil@hive76/member/jmil] has joined ##hplusroadmap14:53
loansharki need to charge my kindle14:53
loansharkany other books?14:53
yashgarothlehninger's biochemistry14:54
yashgarothmolecular biology of the gene14:54
yashgarothhuman molecular genetics14:54
@kanzurei liked campbell biology but that was probably because the alternative (at the time) was holt biology14:54
yashgarothI forget which one we had in school, fuck food chains14:55
@kanzure"do you know what a CONSUMER is?"14:55
loansharkdo i need human molecular genetics?14:56
@kanzuredepends on what you are doing or why14:56
loansharki just like biology14:56
loansharki have no idea what you can/can't do with bioengineering14:56
loansharki thought you could make prozac yogurt :314:57
@kanzurewhy did you think that14:57
yashgaroththat fucking douchebag and his shitass video14:57
loansharkbecause14:57
loansharki searched 'antidepressant' in the registary14:58
loansharkand it came up with some stuff14:58
@kanzurewhat is registary?14:58
-!- augur [~augur@129-2-129-33.wireless.umd.edu] has quit [Remote host closed the connection]14:58
loansharkregistry** -.-14:58
@kanzurewhich registry14:58
loansharkpartsregistry.org14:58
@kanzureok why are you using that site14:59
yashgarothI see a gene activated by a component of st john's wort14:59
@kanzurehttp://partsregistry.org/Part:BBa_K364325:Experience15:00
@kanzurei see a unicode/utf8/charset error15:00
yashgarothoh god I was browsing igem team wikis last night, the UGeorgia team had extremely loud unstoppable gangsta rap on their page15:01
@kanzureigem: the official myspace of synthetic biology15:02
@kanzurebleh15:03
yashgarothanyway loanshark there's very little you can do at the moment outside of a legitimate lab, especially if you have no bio knowledge or experience15:03
@kanzureyou will have to acquire equipment15:04
-!- EnLilaSko [~Nattzor@unaffiliated/enlilasko] has quit [Quit: - nbs-irc 2.39 - www.nbs-irc.net -]15:04
@kanzureand reagents (consumables)15:04
loansharkbut what can you do if you have a small home lab with a PCR and centrifuge and other DIY equipment15:05
yashgarothyou can spend your time finding a company that will actually sell reagents to an individual15:05
yashgarothyou could conceivably sequence parts of your own genome15:06
yashgarothwith an incubator you can make some glowing bacteria15:06
@kanzuremost of the time what happens is that a newbie will pick a huge project15:06
@kanzureand then spend a few months acquiring equipment and planning it out15:06
@kanzureand then realize how impractical it is, and then scale back and pick something more doable15:06
@kanzurewith the equipment already acquired15:07
loansharkcan anyone suggest an feasible project?15:07
yashgarothas in a project where you follow someone else's protocols, or original research?15:08
loansharkfollow someone else's protocols15:09
loanshark:315:09
@kanzureok well pick something: http://protocol-online.org/15:09
yashgaroththere's one where you make bacteria smell like bananas, dunno if they made the gene publicly available15:10
yashgarothsadly it's not yet like computer hacking where the limit is your imagination and expertise15:11
loanshark:315:12
-!- eudoxia [~eudoxia@r186-54-230-60.dialup.adsl.anteldata.net.uy] has joined ##hplusroadmap15:13
eudoxiakanzure, which open source cad program do you see as having the most potential as a component of SKDB15:14
-!- Juul [~Juul@108-222-70-158.lightspeed.sntcca.sbcglobal.net] has joined ##hplusroadmap15:14
eudoxiaor just the best FOSS CAD program right now15:14
@kanzureeudoxia: brlcad is the best-written cad program15:14
@kanzureunfortunately it doesn't support nurbs quite yet15:14
@kanzurei mean, it does in a few places, but not in the ways that matter15:15
@kanzurebut it's definitely superior to openscad15:15
eudoxiai was just going to ask you about that one15:15
@kanzurei don't recommend openscad at all15:16
@kanzurebut http://diyhpl.us/laser_etcher/laser_etcher models are written with it15:16
eudoxiawhy not?15:16
@kanzurethere's no particular reason for its custom language.. that's just silly15:16
@kanzurevariables are a hack or unsupported15:17
@kanzureit's just a tessellation engine really, which you get for free with brlcad or blender, which makes it really not that much of a cad tool..15:17
eudoxiawhat is the standard language for programmatic cad then?15:17
@kanzureautolisp15:17
@kanzureimplicitcad is sorta neat if you're into haskell15:18
loansharkso15:18
loanshark:/15:18
loansharkcould i for example15:18
@kanzureeudoxia: but i'm a big sucker for python cad15:18
@kanzureeudoxia: so stuff like pythonocc (even though opencascade/oce is shit)15:18
@kanzurei mean, opencascade is at least a real cad kernel15:19
@kanzurethe problem is that its quality is very low15:19
eudoxiacan BRLCAD use autolisp?15:19
loansharktake the gene that makes a type of algae glow and put it in another organism?15:19
loanshark 15:19
@kanzurejust none of the other cad offerings really meet that definition ("real cad kernel") except some of the commercial stuff (acis, parasolid, whatever)15:19
@kanzureeudoxia: brlcad has a number of separate libraries that you can wrap into whatever language you want15:19
@kanzureloanshark: no we don't do glowing15:20
eudoxiathat's good15:20
loansharksoo15:20
loanshark:/15:20
loansharkwhat can you do?15:20
@kanzureloanshark: maybe you should learn a little first, and then see if anything gets you interested15:20
@kanzureyou can do anything that's possible15:20
loansharkI'm just trying to find some applications15:21
-!- Juul [~Juul@108-222-70-158.lightspeed.sntcca.sbcglobal.net] has quit [Ping timeout: 260 seconds]15:22
loansharkim really disappointed about the antidepressant thing15:24
eudoxiakanzure: apparently some people have written interfaces to BRLCAD for python, why not use those if openCASCADE is shit?15:24
loansharki thought you made the bacteria produce some chemical that does the same thing that prozac does15:24
loansharkthe only example i can think of is how chemically synthetic THC does the same thing as regular THC15:25
-!- tellurium [~usorid@c-76-23-254-105.hsd1.ct.comcast.net] has joined ##hplusroadmap15:25
@kanzureeudoxia: opencascade can do nurbs cad, brlcad can't (because opennurbs is made by rhino, and rhino keeps the good parts proprietary)15:25
@kanzureeudoxia: i haven't seen the brlcad python wrapper.. can you link me?15:25
eudoxiahttp://forums.reprap.org/read.php?12,14558,1512115:26
@kanzurelolz released on a forum.. yeah uh15:26
@kanzure"That said, is there a reason you aren't using something like ctypes and libwdb for this? If you're just doing compile-style generation of geometry, that's exactly what libwdb is for, and that'd keep you from needing to do anything with TCL or mged at all, and make it easier to add libged's functionality when it gets that far. Looking at ctypes, that should be close to trivial, and certainly easier than mucking about generating input for mged"15:26
@kanzurehmm so he's not using ctypes or cython?15:27
@kanzureyeah so i don't think this is a good idea15:27
@kanzuregenerating input for mged is just as bad as pyopenscadpy15:27
@kanzure(i forget which one; pyopenscad or openscadpy generates input for openscad; the other one is openscad compiled with Python.h)15:28
@kanzureeudoxia: i'd even take a swig-generated interface in python over that15:29
@kanzure(that's what pythonocc was for opencascade, just some swig wrapper around opencascade)15:30
eudoxiaso BRLCAD is good but can't do nurbs and opencascade is shit but can do nurbs15:31
eudoxiahow complicated15:31
@kanzureit gets much more complicated :)15:32
@kanzurelike go look at the opencascade source code15:32
@kanzurehttp://github.com/kanzure/oce15:32
@kanzureand compare it to brlcad (which is heaven in comparison)15:32
-!- Juul [~Juul@108-222-70-158.lightspeed.sntcca.sbcglobal.net] has joined ##hplusroadmap15:34
loansharkkanzure: is there nothing cool you can do with bioengineering in a home lab?15:35
@kanzurethere's tons of cool stuff. you can do genetic engineering.15:36
loansharkwhat can you do with genetic engineering in a home lab?15:37
@kanzurehttp://diyhpl.us/wiki/diybio/faq/projects15:42
loansharkcool thanks :)15:44
loansharki didn't see that15:44
-!- eudoxia [~eudoxia@r186-54-230-60.dialup.adsl.anteldata.net.uy] has quit [Quit: quit]15:44
-!- Juul [~Juul@108-222-70-158.lightspeed.sntcca.sbcglobal.net] has quit [Ping timeout: 260 seconds]15:48
loansharkso15:52
loansharkyou can just grow cultures or build lab equipment?15:52
-!- augur [~augur@208.58.5.87] has joined ##hplusroadmap15:53
loansharkkanzure: what can you do with genetic engineering in a home lab if you can't make SSRI yogurt or organisms that glow15:55
ParahSailinnot much actually16:00
loansharkhttp://partsregistry.org/Part:BBa_K364325:Experience16:00
loansharkthat won't make the bacteria make hyperforin?16:01
-!- Juul [~Juul@108-222-70-158.lightspeed.sntcca.sbcglobal.net] has joined ##hplusroadmap16:01
loansharkhypericin**16:01
@kanzureyou can make organisms glow, but i wouldn't be very supportive16:04
loansharkwhy not?16:05
@kanzurethat page says that PXR is activated by hyperforin..... why do you think this gene product would make hyperforin??16:05
loansharki meant hyericin16:05
loansharkwhich is the SSRI16:05
-!- Juul [~Juul@108-222-70-158.lightspeed.sntcca.sbcglobal.net] has quit [Ping timeout: 245 seconds]16:06
-!- OldCoder [~OldCoder@c-69-181-140-134.hsd1.ca.comcast.net] has joined ##hplusroadmap16:08
OldCoderkanzure, loanshark Hi16:08
OldCoderkanzure, I lost this channel16:08
OldCoderFound it again16:08
loansharkhelllo16:09
OldCoderloanshark, you were looking for a DIY project16:09
loansharkhttp://www.mnn.com/green-tech/research-innovations/photos/12-bizarre-examples-of-genetic-engineering/glow-in-the-dark-c16:10
loansharkthis stuff is cool16:10
OldCoderReviewing16:10
loansharkglow cats!16:10
OldCoderI've read about this16:11
OldCoderBut I wouldn't try this at home! :-)16:11
loansharkwhy not?16:11
OldCoderGengineering kitties?16:12
* OldCoder reads16:12
OldCoderYou don't have the equipment or training needed to do something at this level. Glowing microorganisms are different.16:12
OldCoderYou could make a bottle of pond water glow.16:12
loansharkcould totally set up and operate on kitties16:13
loanshark;316:13
* OldCoder shrugs16:13
loansharkwhat i mean is that that is cool,16:13
OldCoderIt is16:14
OldCoderGlowing tomatoes are useful16:14
loansharklike interesting things that spark my interest in bioengineering and motivate me to learn about it16:14
-!- eridu [~eridu@gateway/tor-sasl/eridu] has joined ##hplusroadmap16:14
OldCoderGood16:14
-!- eridu [~eridu@gateway/tor-sasl/eridu] has quit [Remote host closed the connection]16:14
@kanzureloanshark: no, glowing is NOT cool16:14
@kanzurefuck16:14
@kanzurefuuuck16:14
OldCoder?16:14
OldCoderNot glowing kitties16:14
OldCoderMaybe glowing pond water16:14
OldCoderkanzure, hi16:14
@kanzureit's not even really that interesting: it's just glowing under fluorescent light16:14
loansharkgetting dan from a strawberry with a shot glass for no reason what so ever does not sound very interesting16:14
@kanzureit's not16:15
OldCoderConceptually16:15
chris_99kanzure, what stuff have you done that's cool relating to this that's cool?16:15
loanshark:3 cool stuff16:15
chris_99er, that was poorly worded16:15
@kanzurechris_99: i did some published research on transcriptional logic gates under pcr16:15
loansharkpollution-fighting plants!16:15
loansharkthats cool16:15
OldCoderchris_99, I liked the wording16:15
chris_99link kanzure?16:15
@kanzurechris_99: i don't have a link handy16:16
@kanzurei don't know if it counts as cool though16:16
chris_99is it on scholar?16:16
@kanzureprobably16:16
@kanzurebut i'm not cited16:16
@kanzurebecause i was the disposable undergrad16:16
loansharklogic gates are uncool16:17
OldCoderThat's always fun16:17
loanshark:316:17
chris_99remember the title or other authors?16:17
@kanzurechris_99: yes16:17
@kanzurebut i don't think it matters?16:17
OldCoderkanzure, do you have a Twitter presence?16:17
@kanzureOldCoder: sadly yes http://heybryan.org/ lists it16:18
OldCoderSadly :-)16:18
loansharkhow about a cabbage that makes scorpion venom as a sort of self-pesticide, killing caterpillars that bite it, but not so much that it is harmful to humans16:18
loanshark:316:18
loansharkthat is very cool16:18
@kanzureloanshark: how about you just pick a project you like, instead of hoping someone gives you a ready-made package16:18
loansharki was just asking for ideas and no one had any16:18
@kanzureloanshark: ok so first you should go figure out how to make venom16:18
OldCoderkanzure, PM if all right16:19
@kanzureand then you will see that the expression system probably doesn't work in cabbage16:19
loansharkand someone said there was not much you can do with bio-engineering16:19
@kanzureloanshark: you can do all sorts of things, but that doesn't mean they are easy16:19
OldCoderkanzure, I am already following you. You should tweet more.16:19
@kanzureno i shouldn't16:19
loansharkhttp://www.nature.com/cr/journal/v12/n2/full/7290120a.html16:19
@kanzuredon't encourage me16:19
OldCoderkanzure, do it16:19
loansharkvenomous cabbage already exists16:19
OldCoderdooooooooooo eeeeeeeeeeeeeeeeeet16:19
OldCoder:-)16:19
@kanzureloanshark: that was in ecoli16:20
@kanzureloanshark: if you can replicate that paper's work, then you could start to consider doing it in cabbage16:20
OldCoderkanzure, I'll be here a bit. PM when you can.16:20
@kanzureOldCoder: you may pm me16:20
OldCoderI did16:20
OldCoderTried again16:20
@kanzureok.16:20
loanshark:316:21
loansharkhow about just making things glow16:21
loansharklike worms16:21
@kanzureloanshark: that looks like a chemical synthesis, not a recombinant protein16:21
chris_99anyone think of something funky that requires a vacuum?16:22
loanshark:316:22
@kanzureoh nevermind "AaIT is an excitatory long-chain toxin specific to insects and is composed of 70 amino acids with four disulfide bridges"16:22
@kanzureso it looks like this toxin is just an amino acid chain16:22
browniesuh... what? something funky that requires a vacuum?16:23
chris_99yeah heh, i'm getting a vacuum pump for something else, but was wondering if theres anything else i could use it for16:23
@kanzureum well16:24
@kanzurei'm sure my mom has a16:24
@kanzurei can't do this anymore16:24
browniessuperconducting? cold temperatures in general? fabricating microchips?16:24
@kanzureoh16:24
browniesbasically... everything?16:24
@kanzureis this a 10^-9 torr vacuum? or more?16:24
browniesjust... Scienceā„¢16:24
browniesmm, good question16:24
loansharkcould i inject genes into worm eggs?16:25
chris_990 - 30" Hg apparently16:25
@kanzureyep.. but you would have to follow a protocol if you want genetic manipulation of worm eggs to work16:25
loansharkwhat is a protocol?16:25
@kanzureblack magic16:26
loansharkpublished results of someones research?16:26
@kanzureno16:26
@kanzurehttp://protocol-online.org/16:26
@kanzurethey are list of steps to do things16:26
@kanzurebut usually they are highly specific16:26
loansharkso like a tutorial16:26
@kanzurelike incubate at certain temperatures, use certain amounts of whatever reagents and liquids, etc.16:26
@kanzureno16:26
loansharkrecepies?16:26
@kanzurereally bad recipes16:26
loansharkok16:26
loansharkwill there really be a form egg gene injection protocol?16:27
@kanzurethere might be, if someone has done it before16:28
-!- Juul [~Juul@108-222-70-158.lightspeed.sntcca.sbcglobal.net] has joined ##hplusroadmap16:28
@kanzurebut if not, you will have to spend months/years figuring it out based on related lab techniques and testing16:28
loanshark:316:28
loansharkok16:28
loansharkbecause i might just be killing them16:29
loansharkXD16:29
@kanzureyou will definitely screw up and kill things16:29
loansharkhardcore16:29
loansharkhttp://tropicalis.berkeley.edu/home/manipulate_embryos/microinjection/inject.html16:29
loansharkthe best relating protocol i found - microinjection16:30
-!- superkuh [~superkuh@unaffiliated/superkuh] has quit [Remote host closed the connection]16:31
loansharktheoretically, if you get the single fertilized cell16:32
@kanzureconditions for injecting plasmids into embryos are fairly non-trivial, it varies dramatically by species16:32
loansharkyou can do that electrothingy to open the barriers and then centrifuge cells with glow-in-the dark gene16:32
@kanzureyou are a master of technobabble16:33
loanshark^^ thanks16:33
OldCoderwrite stories16:33
loansharkstories?16:33
-!- chris_99 [~chris_99@unaffiliated/chris-99/x-3062929] has quit [Quit: Leaving]16:33
-!- Falfe [~not@c83-251-81-162.bredband.comhem.se] has quit [Ping timeout: 244 seconds]16:37
loansharkKanzure: if you had the gene that makes luciferase then put that in the worms they would be like fireflies?16:38
@kanzureread the diybio archives about that.. short answer is no16:38
@kanzurehttps://google.com/search?q=diybio+luciferase16:38
loansharkso apparently fluorescence is way easier than bioluminescence16:42
loansharkawww16:44
loansharksynthesizing genes is expensive :(16:44
@kanzureyes dna costs a bit much16:44
@kanzureyashgaroth: maybe diybio is just a clever marketing stunt for carolina's gfp kits16:45
loansharkbut you can just go out and capture some fireflies16:45
yashgarothit's sad how true that could be kanz16:46
-!- Juul [~Juul@108-222-70-158.lightspeed.sntcca.sbcglobal.net] has quit [Ping timeout: 268 seconds]16:50
loansharkhow would you collect fluorescent protein genes from nature?16:54
@kanzureyou would buy some dead squid and pcr it out of their genome with some primers16:55
loansharkok16:56
loanshark:316:56
loansharksee now this is cool :316:56
yashgarothwell they probably have introns, in which case I'd recommend reverse-transcriptase pcr16:56
@kanzureno it's not.. it's just fluorescence dude16:56
loansharkbut16:57
loansharkyour engineering with biology.. building cool things16:57
loansharkchanging them the way you want them16:57
loanshark:316:57
loansharklike lego, but with living things16:58
loansharknow thats cool16:58
@kanzureit's not like lego at all16:59
loansharkits cooler than lego16:59
-!- Juul [~Juul@108-222-70-158.lightspeed.sntcca.sbcglobal.net] has joined ##hplusroadmap17:03
OldCoderLiving Lego17:12
OldCoderHmm17:12
@kanzureJuul: do you have a standard disclaimer for "biobricks don't usually work"17:12
loansharkare primers expensive?17:13
@kanzurethey are synthetic dna17:14
loanshark:/17:14
@kanzureso they cost the same, they are just usually short - about 20 bp to 40 bp or longer ish17:14
loansharkarg!17:14
yashgarotha few dollars each17:14
loansharkoh?17:14
-!- Juul [~Juul@108-222-70-158.lightspeed.sntcca.sbcglobal.net] has quit [Ping timeout: 268 seconds]17:15
yashgarothwell more than a few, but not "same" like several hundred dollars17:16
@kanzuretrue17:16
loanshark4.90 per primer17:17
loanshark:D17:17
-!- Juul [~Juul@108-222-70-158.lightspeed.sntcca.sbcglobal.net] has joined ##hplusroadmap17:28
loansharkhttp://www.operon.com/products/pcr-primers/pcr-primer-overview.aspx?id=pcr17:29
loansharkKanzure $4.90 a primer sounds very affordable17:29
@kanzureand what are you planning to do with these primers17:30
loansharkget the luminescent gene17:30
@kanzurewhy not just use the kit from carolina17:31
@kanzurehttp://diyhpl.us/wiki/diybio/faq/kit17:31
@kanzureoops17:31
@kanzurehttp://diyhpl.us/wiki/diybio/faq/kits17:31
loansharkhow much is it?17:32
@kanzurecheaper than you building your own plasmid system and consuming all those reagents in test17:32
loanshark:/17:34
loansharkreally?17:34
@kanzurewell, do the math and prove me wrong17:34
loansharkidk what it takes to build a plasmid system17:35
@kanzurego find out17:35
loansharkbut wikipedia tells me it is a DNA molecule separate from the chromosomal DNA17:35
@kanzurecorrect17:39
@kanzurethis is one of the gene expression methods17:39
loansharkaaah17:43
loansharkthis stuff is intense17:43
loanshark:317:43
loansharkso17:43
@kanzureloanshark: feel free to edit the diybio faq with your findings17:44
loansharkso you get the DNA17:49
loansharkthen use the DNA to make DNA plasmids?17:49
-!- tashoutang [~tata@pc131090206.ntunhs.edu.tw] has joined ##hplusroadmap17:50
gnushadiyhpluswiki.git: ccaa470 increase instructions for editing the faq17:50
-!- ThomasEgi [~thomas@panda3d/ThomasEgi] has quit [Remote host closed the connection]17:52
tashoutangexcuse me, last time someone give me a clue to search some chemical compound those could be useful after extraction, and can this Mister/Miss give me that clue again?17:58
gnushadiyhpluswiki.git: 18:01
@kanzurejrayhawk: remote: 2012/08/21 18:01:03 socat[29849] E write(3, 0x9da1388, 56): Broken pipe18:01
jrayhawkcurious18:03
gnushahonk honk18:04
jrayhawkwell, beats me18:04
gnushadiyhpluswiki.git: 72e834f add a page about the irc channel18:06
@kanzurehmm the formatting is wacky: http://diyhpl.us/wiki/hplusroadmap#irc18:07
@kanzurei tried \#\#[[hplusroadmap]] but instead i get that^18:08
@kanzureon the other page \#\#[[hplusroadmap]] works.. maybe this is a bug about linking to your own page18:11
gnushadiyhpluswiki.git: 97a74d9 linkify the irc clients list18:14
gnushadiyhpluswiki.git: c2c7913 fix ##hplusroadmap formatting18:16
@kanzurehuh, no, the formatting is still very wrong18:16
browniesthe logs are public? oh man18:18
OldCoderbrownies, I didn't know you were here18:19
browniesOldCoder: i didn't know you were here!18:19
tashoutang@K: did you see my question?18:19
OldCoderI lost the channel18:19
tashoutangexcuse me to bother you @K18:20
brownies< kanzure> but then why did I start off as a moron?18:20
browniesdoesn't take long to find things and take them out context, when you have 4 years of logs18:20
@kanzuretashoutang: yes i saw your question. there are many chemical compounds. i'm not sure if i told you any.18:23
gnushadiyhpluswiki.git: c9058b7 better instructions for editing the wiki18:24
tashoutangoooo, and in private I ask you that are u talking about the website setting with html language18:24
@kanzureah i see.18:25
@kanzurewell, no, this is not quite html18:25
@kanzureit's markdown being converted to html18:25
tashoutanghaha I am still not very familiar with mirc18:25
tashoutangOK18:25
@kanzureno it worked, but i am in too many irc channels and do not see private notifications easily :)18:26
tashoutangI see18:26
tashoutangthanks!:)18:26
brownieskanzure: what client do you use?18:26
gnushadiyhpluswiki.git: 775970d describe the wiki in the intro page18:27
@kanzurebrownies: irssi18:27
-!- Juul [~Juul@108-222-70-158.lightspeed.sntcca.sbcglobal.net] has quit [Ping timeout: 268 seconds]18:28
gnushadiyhpluswiki.git: 8caf394 list out some interesting pages18:31
tashoutangon DIYbio someone said they want to start a DIYbio project whitch include each individual around the world, and welcome every one to offer its idea, and the one who propose this poster said he prefer to plane a project to build a creature and put it on the mars.18:37
gnushadiyhpluswiki.git: 8d9a3d1 move text files to wiki files in transcripts/startup-science-201218:37
@kanzuretashoutang: yes that was funny18:37
tashoutangI know that scientist had built artificial micoplasma...\18:38
@kanzurebrownies: these might be slightly readable now.. http://diyhpl.us/wiki/transcripts/startup-science-2012/18:38
@kanzureoh crap, nope18:38
tashoutangwhy they just start to simulate the surviving ability from that creature first?18:38
gnushadiyhpluswiki.git: b089f36 convert transcripts/startup-science-2012 to markdown18:39
@kanzurebrownies: ok try now.18:39
* brownies looks18:41
brownieskanzure: much better. the whole site is still too wide.18:41
browniesjam the whole thing into a 940px div. that would be a huge step forward.18:41
@kanzurecan you pop open chrome inspector and suggest a --18:41
@kanzureok 940px div.. will try that18:42
@kanzurewait do i care18:42
gnushadiyhpluswiki.git: 1f31d45 restrict the body element to 940px18:44
@kanzurebrownies: how about now?18:44
brownieskanzure: much better.18:44
@kanzureblah the title stuff isn't in a block div http://diyhpl.us/wiki/transcripts/startup-science-2012/carlo-quinonez/18:44
brownieskanzure: just throw that search bar next to the links18:45
-!- eridu [~eridu@gateway/tor-sasl/eridu] has joined ##hplusroadmap19:08
-!- eridu [~eridu@gateway/tor-sasl/eridu] has quit [Quit: Leaving]19:17
-!- roksprok [~Zac@50-0-91-31.dsl.dynamic.sonic.net] has joined ##hplusroadmap19:18
-!- roksprok [~Zac@50-0-91-31.dsl.dynamic.sonic.net] has quit [Client Quit]19:19
-!- ivan`_ [~ivan@unaffiliated/ivan/x-000001] has quit [Ping timeout: 276 seconds]20:06
-!- ivan` [~ivan@unaffiliated/ivan/x-000001] has joined ##hplusroadmap20:07
-!- ivan` [~ivan@unaffiliated/ivan/x-000001] has quit [Ping timeout: 260 seconds]20:26
-!- ivan` [~ivan@unaffiliated/ivan/x-000001] has joined ##hplusroadmap20:29
-!- ivan` [~ivan@unaffiliated/ivan/x-000001] has quit [Ping timeout: 260 seconds]20:43
-!- nmz787 [~Nathan@107.53.239.200] has joined ##hplusroadmap20:44
-!- ivan` [~ivan@unaffiliated/ivan/x-000001] has joined ##hplusroadmap20:45
@kanzurenmz787: how was maradydd?20:47
nmz787she didn't end up coming to hackmanhattan, said she was still jetlagged20:49
nmz787and not feeling well20:49
-!- jmil [~jmil@hive76/member/jmil] has quit [Ping timeout: 272 seconds]20:54
-!- superkuh [~superkuh@unaffiliated/superkuh] has joined ##hplusroadmap21:03
@kanzurethis seems sorta absurd https://community.rapid7.com/community/metasploit/blog/2012/08/21/metasploit-portable-ios21:06
-!- mensch [~mensch@c-75-69-228-244.hsd1.ma.comcast.net] has quit [Quit: leaving]21:23
-!- sylph_mako [~mako@84.35.252.27.dyn.cust.vf.net.nz] has joined ##hplusroadmap21:31
-!- sylph_mako [~mako@84.35.252.27.dyn.cust.vf.net.nz] has quit [Read error: Connection reset by peer]21:32
-!- nmz7871 [~Nathan@ool-457e5cbd.dyn.optonline.net] has joined ##hplusroadmap21:43
-!- loanshark [~loanshark@ip72-218-133-8.hr.hr.cox.net] has quit [Quit: loanshark]21:43
-!- nmz787 [~Nathan@107.53.239.200] has quit [Ping timeout: 260 seconds]21:47
-!- nmz787 [~Nathan@107.53.239.200] has joined ##hplusroadmap21:47
-!- nmz7871 [~Nathan@ool-457e5cbd.dyn.optonline.net] has quit [Ping timeout: 260 seconds]21:50
-!- tellurium [~usorid@c-76-23-254-105.hsd1.ct.comcast.net] has quit [Quit: <neopolitan> I come here to insult and troll and ban people and have fun doing it. i can't be distracted from my playtime with these philosophical discussions]22:13
-!- Mokbortolan_1 [~Nate@c-76-115-136-13.hsd1.or.comcast.net] has quit [Read error: Connection reset by peer]22:20
-!- Mokbortolan_ [~Nate@c-76-115-136-13.hsd1.or.comcast.net] has joined ##hplusroadmap22:22
-!- OldCoder is now known as YoungCoder22:29
-!- YoungCoder is now known as LittleCoder22:29
-!- LittleCoder is now known as GoldCoder22:30
-!- GoldCoder is now known as SleepyCoder22:30
-!- SleepyCoder is now known as ColdCoder22:31
-!- ColdCoder is now known as OldCoder22:31
nmz787this looks like a spicy meatball: "Gene Synthesis: Methods and Protocols (Methods in Molecular Biology, 852) 2012 Jean Peccoud" http://libgen.info/view.php?id=82367322:33
yashgaroth"Leading a Successful iGEM Team"22:36
@kanzureif that's not written by slovenian nationalists i would be suspicious of it22:36
@kanzurepastebin for math: http://mathb.in/22:39
@kanzuremaybe there should be a genetic pastebin called genebin22:39
@kanzureit could even have shitty annotations and shitty plasmid visualization22:39
browniesi thought there was already a latexbin?22:40
browniesalthough admittedly this looks way, way better22:40
@kanzurewhat's latexbin like?22:40
browniesfeels like 199622:41
@kanzure"pastebinit" used to be a great little tool until pastebin.com changed their api22:41
@kanzureand now pastebinit requires -b http://paste.ubuntu.com/ or some other pastebin tacked on.. kinda ruins the point if you have to make a custom alias22:41
brownieskanzure: couldn't you just alias it once in your bash profile and leave it at that...22:42
browniesoh i see. that's too much work for you. alright.22:42
@kanzurewell, that's what i've done, yes22:43
-!- Mokbortolan_ [~Nate@c-76-115-136-13.hsd1.or.comcast.net] has quit [Read error: Connection reset by peer]22:47
-!- Mokbortolan_ [~Nate@c-76-115-136-13.hsd1.or.comcast.net] has joined ##hplusroadmap22:48
-!- nmz7871 [~Nathan@ool-457e5cbd.dyn.optonline.net] has joined ##hplusroadmap22:48
-!- nmz787 [~Nathan@107.53.239.200] has quit [Ping timeout: 260 seconds]22:51
@kanzurehttp://blog.mylookout.com/_media/Geinimi_Trojan_Teardown.pdf23:09
-!- Mokbortolan_ [~Nate@c-76-115-136-13.hsd1.or.comcast.net] has quit [Read error: Connection reset by peer]23:20
-!- Mokbortolan_ [~Nate@c-76-115-136-13.hsd1.or.comcast.net] has joined ##hplusroadmap23:21
-!- nmz7871 [~Nathan@ool-457e5cbd.dyn.optonline.net] has quit [Quit: Leaving.]23:50
-!- nmz787 [~Nathan@ool-457e5cbd.dyn.optonline.net] has joined ##hplusroadmap23:51
-!- tellurium [~usorid@c-76-23-254-105.hsd1.ct.comcast.net] has joined ##hplusroadmap23:53
--- Log closed Wed Aug 22 00:00:03 2012

Generated by irclog2html.py 2.15.0.dev0 by Marius Gedminas - find it at mg.pov.lt!