2013-02-12.log

--- Log opened Tue Feb 12 00:00:41 2013
--- Day changed Tue Feb 12 2013
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strangewarpI liked the Glitch Feminist Manifesto, if only because the claim that glitches are little orgasms was fascinating01:11
archelswhat is 'glitch' in this context?01:19
archels"When the computer freezes mid-conversation, when the video buffers and refuses to progress, these moments are a new mode of foreplay, something that needs to be acknowledged not as a fetish, but as new possibly for play within sexual routine."01:25
archelso-kay...01:25
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@kanzurearchels: welcome to the weird part of the internet01:37
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ParahSailinwhy would one want to get beta carotene into e coli cell?08:56
chris_99why not put it in yeast instead if thats possible08:57
ParahSailinno, i mean i dont understand the intent at all08:58
ParahSailinwhy would you want to put beta carotene in yeast08:58
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chris_99no idea, at least it'd then be edible though i guess :) to make a beer with more vitamins in? ;)09:00
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ThomasEgibeta carotene? isnt that the orange stuff?. making orange beer?09:27
chris_99oh cool it is orange :) didn't realise that09:33
chris_99http://www.ncbi.nlm.nih.gov/pmc/articles/PMC1932764/09:36
chris_99doesn't sound easy though09:36
nmz787archels: this glitch foreplay crazy, yesterday my computer totally froze, like completely... i gave it a minute to unfreeze, saw the hard drive light blinking once in a while... but it was glitching too hard on me and i had to hard reset09:39
nmz787...09:39
nmz787yeah, glitches don't really seem to turn me on at all09:39
chris_99say for instance i wanted to make glowing beer, what would be the easiest way to do it, could i use electroporation09:42
nmz787sure09:43
nmz787electroporation is great09:43
nmz787i'm a strong advocate09:43
nmz787glowing beer is tough tho09:43
nmz787there's a lot of engineering that's prob needed09:43
nmz787since apparently the known glowGenes require oxygen, and beer is generally more anoxic09:44
chris_99some yeast is actually aerobic if that helps?09:44
nmz787chris_99: they can grow aerobically sure09:47
nmz787but they won't ferment alcohol09:47
chris_99Brettanomyces does iirc09:47
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@kanzure"patent avoidance resources" http://www.tinaja.com/pasamp1.shtml09:50
@kanzurenmz787: "yeah, glitches don't really seem to turn me on at all" "sure, electroporation is great"09:54
nmz787"Another use for cinnamaldehyde is as an antimicrobial. Researchers from the University of Illinois at Chicago (who were funded by the Wm. Wrigley Jr. Company) have found that cinnamic aldehyde, when used in Big Red, prevented oral bacterial growth by more than 50%.[9] It is especially effective against bacteria living at the back of the tongue, reducing anaerobic bacteria populations by about 43%."09:55
nmz787kanzure: but electroporation isn't a glitch09:55
nmz787i guess those0 sentences are still weird09:56
nmz787"Cinnamaldehyde is also used as a fungicide.[5] Proven effective on over 40 different crops, cinnamaldehyde is typically applied to the root systems of plants. Its low toxicity and well-known properties make it ideal for agriculture. Cinnamaldehyde is an effective insecticide, and its scent is also known to repel animals, such as cats and dogs.[5] It has recently been recognized as a very effective insecticide for mosquito larvae.[6] As little as 29 09:56
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nmz787"Coumarin is often found in artificial vanilla substitutes, despite having been banned as a food additive in numerous countries since the mid-20th century. Coumarin was banned as a food additive in the United States in 1954, largely because of hepatotoxicity results in rodents."09:58
nmz787huh, well I guess no more imitation vanilla extract09:58
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nmz787"I very strongly feel those "patent it yourself" books are a10:06
nmz787big disservice to most individuals and small scale startups.10:06
nmz787Because they encourage wasting your time and money only10:06
nmz787to get a totally useless result."10:06
nmz787"your costs of getting a patent end up utterly negligible10:06
nmz787compared to your costs of defending a patent"10:06
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nmz787so it seems these reagent companies can deliver hazardous materials to residential addressess just fine10:11
nmz787this company accidentally sent stuff to my home address even though I gave them the lab address10:11
nmz787kanzure: just checking but is this filed http://publicintelligence.net/ufouo-dhs-lawrence-livermore-unconventional-biological-laboratory-guide/10:17
nmz787pdf: http://info.publicintelligence.net/DHSunconventionalbiolabs.pdf10:17
nmz787date: 2009-07-2410:17
nmz787paperbot http://info.publicintelligence.net/DHSunconventionalbiolabs.pdf10:18
@paperbotno translator available, raw dump: http://diyhpl.us/~bryan/papers2/paperbot/7d471dde57f83432846ef4537b4dbf85.pdf10:19
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nmz787ahh yes, this is the post that stands out in my mind https://groups.google.com/d/msg/diybio/VeUyo6Bh-Xo/NrHOXLJDIB4J10:25
nmz787"If a company sells any10:25
nmz787restricted items (chemicals, cultures, whatever), they are likely to10:25
nmz787never ship anything to a residence, even if all you want are some10:25
nmz787plastic tube racks.  In some cases (cough, VWR, cough), any attempt10:25
nmz787for a hobbyist to even set up an account results in a visit from law10:25
nmz787enforcement with a seach warrant.  Same with Invitrogen. "10:25
nmz787bwah hah hah, seems this person doesn't know how to work the system10:26
nmz787"45% Off New Year Sale!" "Potassium cyanide" "Minimum Order: $50"10:31
@kanzureyou mean they were lying?10:33
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nmz787or just wrong, or they set off some alarms by some other action???10:44
juri_sounds like a theory i'm not testing.10:53
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@kanzure"perl problems" http://xkcd.com/1171/11:17
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nmz787juri_: it's no theory, it's plain incorrect11:41
BioGuyKanzure I found a place near me called American Scientific ( amersci.com ), they were happy to sell chemical reagents to me, order something if they didn't usually sell it, and repackage if I wanted something smaller than stock quantities.11:41
nmz787BioGuy: cool11:42
nmz787BioGuy: busy?11:42
nmz787i just got off the phone with edmund optics.... man this stuff is expensive11:42
BioGuyYa need to head down to the mall 205 area11:42
nmz787mall?11:42
nmz787i haven't been to one of those in a while11:43
BioGuyYa, off of I-205 and stark street11:45
nmz787you're over near the airport?11:46
nmz787i thought you were in beaverton11:46
BioGuyI am, gotta drive out there.11:47
BioGuyYou ever go to sleep :-P11:48
nmz787yeah11:48
nmz787got up at 8 feelin pretty good11:48
BioGuyAt least you get back on track easily. Once I mess with my sleep schedule its like a whole month before I can wake up at a normal time again11:52
BioGuyWell gotta run, that article you linked to on publicintelligence.net is pretty interesting. I'll have to read it over more when I get back.11:54
BioGuyI was never sure if it was just policy of most companies not to ship supplies to home addresses or a law.11:54
nmz787kanzure: am I a Formall educated expert, or an Accredited professional?11:57
nmz787Formally*11:57
nmz787BioGuy: that article isn't really related to shipping stuff11:57
nmz787BioGuy: I just happened to get some sequencing kits and acrylamide gel delivered to my front door this morning11:58
nmz787BioGuy: and thought that person's statement was super ironic11:58
nmz787BioGuy: I've never had any trouble with companies, and have received thousands of dollars in free reagents11:58
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BioGuyI remember seeing a few companies online that (I think) sold reagents to home addresses so I think it may just be the policy of most suppliers. Maybe the logic is something like, "Why take $10 worth of risk for potentially $5 profit"12:01
BioGuyI tried ordering something from Carolina a while back, but they said they would only sell to academic institutions or businesses.12:01
nmz787BioGuy: maybe I just know how to talk to people12:02
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BioGuyI guess...12:06
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BioGuySo far I havent had any problems with local suppliers where I can actually walk in and talk to people. Just have ran into barriers online.12:11
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@kanzurenmz787: you are a formally accredited expert, in fact, full expert accreditation for everyone!12:18
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nmz787kanzure: these deskgen.com guy's got 7.5 mil from somewhere to do desktop dna synth http://www.linkedin.com/in/rileyodoyle14:14
@kanzurehttp://webcache.googleusercontent.com/search?q=cache%3Ahttp%3A%2F%2Fwww.deskgen.com%2Fwp-content%2Fuploads%2F2013%2F02%2FDeskGenExecutiveSummaryUK05Feb.pdf&oq=cache%3Ahttp%3A%2F%2Fwww.deskgen.com%2Fwp-content%2Fuploads%2F2013%2F02%2FDeskGenExecutiveSummaryUK05Feb.pdf&aqs=chrome.0.57j58.1045&sourceid=chrome&ie=UTF-814:16
@kanzurei wonder how they got the 1.1 million genes number14:18
@kanzureryan bethencourt seems to know him14:19
nmz787i wonder what they're doing, yeah, is he fishing for ideas?14:19
@kanzurewow they are pennypenchers14:20
@kanzurehttps://angel.co/desktop-genetics#recruiting14:20
@kanzure$35k for "lead software engineer"14:20
nmz787"because its recipes minimize theuse of highly error-prone (and expensive) synthetic oligonucleotides (the greatestsource of non-user error in gene assembly)"14:20
nmz787kanzure: 4^10 is just shy of 1.1 million14:21
nmz787so they may just be using 10mer library14:21
@kanzurei dunno if you remember the survey they were emailing around14:21
@kanzurehttps://www.surveymonkey.com/s/plasmids14:21
nmz787maybe we can sue them14:21
@kanzurewhy would you sue them? on what grounds?14:21
klafkado you guys know that surveymonkey has like 400 million in VC funding14:22
@kanzuresounds familiar14:22
klafkaidk it's still pretty crazy to me14:22
nmz787kanzure: we came up with that library and nicking enzyme idea... just a thought14:22
@kanzurenothing here indicates that they are using a nicking enzyme14:23
@kanzureand we did not invent the concept of dna libraries -_-14:23
@kanzurefurthermore, we don't have a patent on that concept either.14:23
nmz787kanzure: just a thought14:24
nmz787it was supposed to be a fun thought14:24
nmz787their progress to date and exit strategy is interesting14:24
nmz787well planned out14:25
@kanzuretheir progress is just some software and fundraising14:25
nmz787by the sound of it14:25
nmz787yeah that's kinda weird14:25
@kanzure"make an exit to a specific company" is not "well planned out" dude14:25
nmz787it's not clear when the autoclone software becomes useful14:25
@kanzurethat's "wishful thinking". anybody can say "and then google will buy us for $100 million."14:25
nmz787i mean their estimates14:25
nmz787it doesn't say a specific company14:26
nmz787spelling error on this page jumps out http://www.deskgen.com/get-involved/14:28
nmz787'Enrol'14:28
@kanzure"ynthBots communicate with each other and computers over a network, sending and receiving DNA data like a fax machine so that collaborators will always have the latest version of your DNA."14:45
@kanzurecomparing your product to a fax machine seems sort of archaic..14:45
@kanzureanyway, as far as i can tell, they don't have a prototype.14:46
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ParahSailinlol pay someone, living in london no less, 35k for lead X14:51
@kanzurefor on-site work.14:51
@kanzurelondon is notorious for underpaying their programmers14:51
@kanzurebut this is a big stretch.14:51
ParahSailinor is that 0-10% implying equity?14:51
@kanzureyes that implies negotiable equity14:52
ParahSailinstill, london is fucking expensive to live in14:52
ParahSailini'd be losing money with that pretax salary there14:52
@kanzurei think salaries in the UK might be described in post-tax terms, but i'm not sure.14:52
@kanzurestill a bad deal though. if they think paying someone $35k is good enough for their startup then they must not have a good sense of value.14:53
klafkafuck that14:58
klafka35k pounds?14:58
@kanzurenmz787: hah, do you remember this? https://groups.google.com/forum/?fromgroups#!topic/diybio/nMGVN_xve-E15:09
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@kanzurenmz787: where is your evidence that they raised $7.5M?15:19
@kanzurethey got $7,500 from a business plan competition, and another $10-15k from an incubator.15:20
klafkathat's like...nothing15:20
@kanzureyes. that's not $7.5M.15:20
@kanzure"The British company is currently beta testing the software and expects to be able to launch the SynthBot for construction by 2015."15:33
@kanzurefrom http://mobihealthnews.com/19957/healthbox-london-holds-demo-day-irish-accelerator-healthxl-launches/15:33
@kanzurewhat does that even mean o_o you don't "launch" a product by building the first prototype..15:35
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nmz787_Kanzure you're right it was 7500 not 616:36
nmz787_Not 7.5 mil16:36
nmz787_*16:36
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curtisshttps://en.wikipedia.org/wiki/File:Cloned_mice_with_different_DNA_methylation.png18:49
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@kanzurethis seems unreasonably expensive:19:55
@kanzurehttp://www.ebay.com/itm/BAUM-Humanware-Brailliant-32-portable-bluetooth-USB-Braille-display-/17093382740219:55
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phmCurious. Why are you looking at it?19:59
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sylph_mako_origiIt could be useful for a wearable. Possibly.20:21
sylph_mako_origiIf you explicitly wanted to avoid having to use a HMD.20:22
superkuhI have a friend that managed to get one of those humanware setups secondhand; would've been $4k normally.20:22
@kanzurehow much did he pay for his secondhand unit?20:22
superkuhI think it was free.20:22
superkuhBut they're pretty much blind.20:23
@kanzuretheoretically the reason it's so expensive is because there's not enough blind people that would buy one, so the price has to be great enough to subsidize whatever sillyheaded practices the business making them is conducting.20:24
@kanzurei can't imagine the individual braille display units being expensive themselves20:24
superkuhApparently the old models like the one in the ebay link have horrible bluetooth implementations. The new $4k-$6k ones work properly.20:30
@kanzurewhy are there so few characters provided?20:37
@kanzurewhy not 100+ braille characters at a time?20:37
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@kanzuresecret prior art http://news.ycombinator.com/item?id=521155921:57
@kanzurewas FEI the original OEM of electron microscopes?22:23
@kanzurenope, nevermind.22:28
@kanzure"Theodor Svedberg invented the analytical ultracentrifuge in 1925,[1] and won the Nobel Prize in Chemistry in 1926 for his research on colloids and proteins using the ultracentrifuge."22:31
@kanzure"Ernst Ruska at the University of Berlin, along with Max Knoll, combined these characteristics and built the first transmission electron microscope (TEM) in 1931, for which Ruska was awarded the Nobel Prize for Physics in 1986."22:31
@kanzure i think there's an "equipment bias" in nobel prizes :P22:32
@kanzure" Instead, what Bardeen, Brattain, and Shockley invented in 1947 was the first bipolar point-contact transistor.[6] In acknowledgement of this accomplishment, Shockley, Bardeen, and Brattain were jointly awarded the 1956 Nobel Prize in Physics "for their researches on semiconductors and their discovery of the transistor effect."[11]"22:33
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@kanzure"SciQuest.com(TM), Inc. (Nasdaq: SQST), a leading business-to-business e-marketplace for the global scientific products industry, announced today the launch of a cutting-edge laboratory equipment leasing program via the SciQuest.com Auctions site."23:13
@kanzure"Under the terms of this three year partnership, Vista Capital will contribute the resources to pre-approve SciQuest.com Auction users for leases of up to $75,000 with no financial information required."23:14
@kanzure"The dollar value of the line is embedded into the software so that each company registrant can see the remaining line of credit in real-time."23:14
@kanzurehttp://www.thefreelibrary.com/SciQuest.com+Partners+with+Vista+Capital+Corporation+to+Create...-a06005727623:15
@kanzurereading that because of this,23:15
@kanzurehttp://iubio.bio.indiana.edu:7131/bionet/mm/cellbiol/1998-June/008901.html23:15
@kanzure(this company was acquired by sciquest i think)23:15
@kanzurepfft they were an ibm poster-child ftp://ftp.support.lotus.com/software/solutions/pdfs/g325-6614-01.pdf23:18
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@kanzure"synthetic genomics' main line of business right now is licensing out gibson assembly"23:38
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@kanzure"In 1995, upon Andre Marion retirement, Mike Hunkapiller became President of PE Applied Biosystems Division which sold its 30,000th thermal cycler."23:55
--- Log closed Wed Feb 13 00:00:59 2013

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