--- Log opened Tue Feb 12 00:00:41 2013 | ||
--- Day changed Tue Feb 12 2013 | ||
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strangewarp | I liked the Glitch Feminist Manifesto, if only because the claim that glitches are little orgasms was fascinating | 01:11 |
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archels | what is 'glitch' in this context? | 01:19 |
archels | "When the computer freezes mid-conversation, when the video buffers and refuses to progress, these moments are a new mode of foreplay, something that needs to be acknowledged not as a fetish, but as new possibly for play within sexual routine." | 01:25 |
archels | o-kay... | 01:25 |
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@kanzure | archels: welcome to the weird part of the internet | 01:37 |
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ParahSailin | why would one want to get beta carotene into e coli cell? | 08:56 |
chris_99 | why not put it in yeast instead if thats possible | 08:57 |
ParahSailin | no, i mean i dont understand the intent at all | 08:58 |
ParahSailin | why would you want to put beta carotene in yeast | 08:58 |
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chris_99 | no idea, at least it'd then be edible though i guess :) to make a beer with more vitamins in? ;) | 09:00 |
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ThomasEgi | beta carotene? isnt that the orange stuff?. making orange beer? | 09:27 |
chris_99 | oh cool it is orange :) didn't realise that | 09:33 |
chris_99 | http://www.ncbi.nlm.nih.gov/pmc/articles/PMC1932764/ | 09:36 |
chris_99 | doesn't sound easy though | 09:36 |
nmz787 | archels: this glitch foreplay crazy, yesterday my computer totally froze, like completely... i gave it a minute to unfreeze, saw the hard drive light blinking once in a while... but it was glitching too hard on me and i had to hard reset | 09:39 |
nmz787 | ... | 09:39 |
nmz787 | yeah, glitches don't really seem to turn me on at all | 09:39 |
chris_99 | say for instance i wanted to make glowing beer, what would be the easiest way to do it, could i use electroporation | 09:42 |
nmz787 | sure | 09:43 |
nmz787 | electroporation is great | 09:43 |
nmz787 | i'm a strong advocate | 09:43 |
nmz787 | glowing beer is tough tho | 09:43 |
nmz787 | there's a lot of engineering that's prob needed | 09:43 |
nmz787 | since apparently the known glowGenes require oxygen, and beer is generally more anoxic | 09:44 |
chris_99 | some yeast is actually aerobic if that helps? | 09:44 |
nmz787 | chris_99: they can grow aerobically sure | 09:47 |
nmz787 | but they won't ferment alcohol | 09:47 |
chris_99 | Brettanomyces does iirc | 09:47 |
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@kanzure | "patent avoidance resources" http://www.tinaja.com/pasamp1.shtml | 09:50 |
@kanzure | nmz787: "yeah, glitches don't really seem to turn me on at all" "sure, electroporation is great" | 09:54 |
nmz787 | "Another use for cinnamaldehyde is as an antimicrobial. Researchers from the University of Illinois at Chicago (who were funded by the Wm. Wrigley Jr. Company) have found that cinnamic aldehyde, when used in Big Red, prevented oral bacterial growth by more than 50%.[9] It is especially effective against bacteria living at the back of the tongue, reducing anaerobic bacteria populations by about 43%." | 09:55 |
nmz787 | kanzure: but electroporation isn't a glitch | 09:55 |
nmz787 | i guess those0 sentences are still weird | 09:56 |
nmz787 | "Cinnamaldehyde is also used as a fungicide.[5] Proven effective on over 40 different crops, cinnamaldehyde is typically applied to the root systems of plants. Its low toxicity and well-known properties make it ideal for agriculture. Cinnamaldehyde is an effective insecticide, and its scent is also known to repel animals, such as cats and dogs.[5] It has recently been recognized as a very effective insecticide for mosquito larvae.[6] As little as 29 | 09:56 |
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nmz787 | "Coumarin is often found in artificial vanilla substitutes, despite having been banned as a food additive in numerous countries since the mid-20th century. Coumarin was banned as a food additive in the United States in 1954, largely because of hepatotoxicity results in rodents." | 09:58 |
nmz787 | huh, well I guess no more imitation vanilla extract | 09:58 |
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nmz787 | "I very strongly feel those "patent it yourself" books are a | 10:06 |
nmz787 | big disservice to most individuals and small scale startups. | 10:06 |
nmz787 | Because they encourage wasting your time and money only | 10:06 |
nmz787 | to get a totally useless result." | 10:06 |
nmz787 | "your costs of getting a patent end up utterly negligible | 10:06 |
nmz787 | compared to your costs of defending a patent" | 10:06 |
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nmz787 | so it seems these reagent companies can deliver hazardous materials to residential addressess just fine | 10:11 |
nmz787 | this company accidentally sent stuff to my home address even though I gave them the lab address | 10:11 |
nmz787 | kanzure: just checking but is this filed http://publicintelligence.net/ufouo-dhs-lawrence-livermore-unconventional-biological-laboratory-guide/ | 10:17 |
nmz787 | pdf: http://info.publicintelligence.net/DHSunconventionalbiolabs.pdf | 10:17 |
nmz787 | date: 2009-07-24 | 10:17 |
nmz787 | paperbot http://info.publicintelligence.net/DHSunconventionalbiolabs.pdf | 10:18 |
@paperbot | no translator available, raw dump: http://diyhpl.us/~bryan/papers2/paperbot/7d471dde57f83432846ef4537b4dbf85.pdf | 10:19 |
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nmz787 | ahh yes, this is the post that stands out in my mind https://groups.google.com/d/msg/diybio/VeUyo6Bh-Xo/NrHOXLJDIB4J | 10:25 |
nmz787 | "If a company sells any | 10:25 |
nmz787 | restricted items (chemicals, cultures, whatever), they are likely to | 10:25 |
nmz787 | never ship anything to a residence, even if all you want are some | 10:25 |
nmz787 | plastic tube racks. In some cases (cough, VWR, cough), any attempt | 10:25 |
nmz787 | for a hobbyist to even set up an account results in a visit from law | 10:25 |
nmz787 | enforcement with a seach warrant. Same with Invitrogen. " | 10:25 |
nmz787 | bwah hah hah, seems this person doesn't know how to work the system | 10:26 |
nmz787 | "45% Off New Year Sale!" "Potassium cyanide" "Minimum Order: $50" | 10:31 |
@kanzure | you mean they were lying? | 10:33 |
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nmz787 | or just wrong, or they set off some alarms by some other action??? | 10:44 |
juri_ | sounds like a theory i'm not testing. | 10:53 |
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@kanzure | "perl problems" http://xkcd.com/1171/ | 11:17 |
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nmz787 | juri_: it's no theory, it's plain incorrect | 11:41 |
BioGuy | Kanzure I found a place near me called American Scientific ( amersci.com ), they were happy to sell chemical reagents to me, order something if they didn't usually sell it, and repackage if I wanted something smaller than stock quantities. | 11:41 |
nmz787 | BioGuy: cool | 11:42 |
nmz787 | BioGuy: busy? | 11:42 |
nmz787 | i just got off the phone with edmund optics.... man this stuff is expensive | 11:42 |
BioGuy | Ya need to head down to the mall 205 area | 11:42 |
nmz787 | mall? | 11:42 |
nmz787 | i haven't been to one of those in a while | 11:43 |
BioGuy | Ya, off of I-205 and stark street | 11:45 |
nmz787 | you're over near the airport? | 11:46 |
nmz787 | i thought you were in beaverton | 11:46 |
BioGuy | I am, gotta drive out there. | 11:47 |
BioGuy | You ever go to sleep :-P | 11:48 |
nmz787 | yeah | 11:48 |
nmz787 | got up at 8 feelin pretty good | 11:48 |
BioGuy | At least you get back on track easily. Once I mess with my sleep schedule its like a whole month before I can wake up at a normal time again | 11:52 |
BioGuy | Well gotta run, that article you linked to on publicintelligence.net is pretty interesting. I'll have to read it over more when I get back. | 11:54 |
BioGuy | I was never sure if it was just policy of most companies not to ship supplies to home addresses or a law. | 11:54 |
nmz787 | kanzure: am I a Formall educated expert, or an Accredited professional? | 11:57 |
nmz787 | Formally* | 11:57 |
nmz787 | BioGuy: that article isn't really related to shipping stuff | 11:57 |
nmz787 | BioGuy: I just happened to get some sequencing kits and acrylamide gel delivered to my front door this morning | 11:58 |
nmz787 | BioGuy: and thought that person's statement was super ironic | 11:58 |
nmz787 | BioGuy: I've never had any trouble with companies, and have received thousands of dollars in free reagents | 11:58 |
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BioGuy | I remember seeing a few companies online that (I think) sold reagents to home addresses so I think it may just be the policy of most suppliers. Maybe the logic is something like, "Why take $10 worth of risk for potentially $5 profit" | 12:01 |
BioGuy | I tried ordering something from Carolina a while back, but they said they would only sell to academic institutions or businesses. | 12:01 |
nmz787 | BioGuy: maybe I just know how to talk to people | 12:02 |
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BioGuy | I guess... | 12:06 |
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BioGuy | So far I havent had any problems with local suppliers where I can actually walk in and talk to people. Just have ran into barriers online. | 12:11 |
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@kanzure | nmz787: you are a formally accredited expert, in fact, full expert accreditation for everyone! | 12:18 |
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nmz787 | kanzure: these deskgen.com guy's got 7.5 mil from somewhere to do desktop dna synth http://www.linkedin.com/in/rileyodoyle | 14:14 |
@kanzure | http://webcache.googleusercontent.com/search?q=cache%3Ahttp%3A%2F%2Fwww.deskgen.com%2Fwp-content%2Fuploads%2F2013%2F02%2FDeskGenExecutiveSummaryUK05Feb.pdf&oq=cache%3Ahttp%3A%2F%2Fwww.deskgen.com%2Fwp-content%2Fuploads%2F2013%2F02%2FDeskGenExecutiveSummaryUK05Feb.pdf&aqs=chrome.0.57j58.1045&sourceid=chrome&ie=UTF-8 | 14:16 |
@kanzure | i wonder how they got the 1.1 million genes number | 14:18 |
@kanzure | ryan bethencourt seems to know him | 14:19 |
nmz787 | i wonder what they're doing, yeah, is he fishing for ideas? | 14:19 |
@kanzure | wow they are pennypenchers | 14:20 |
@kanzure | https://angel.co/desktop-genetics#recruiting | 14:20 |
@kanzure | $35k for "lead software engineer" | 14:20 |
nmz787 | "because its recipes minimize theuse of highly error-prone (and expensive) synthetic oligonucleotides (the greatestsource of non-user error in gene assembly)" | 14:20 |
nmz787 | kanzure: 4^10 is just shy of 1.1 million | 14:21 |
nmz787 | so they may just be using 10mer library | 14:21 |
@kanzure | i dunno if you remember the survey they were emailing around | 14:21 |
@kanzure | https://www.surveymonkey.com/s/plasmids | 14:21 |
nmz787 | maybe we can sue them | 14:21 |
@kanzure | why would you sue them? on what grounds? | 14:21 |
klafka | do you guys know that surveymonkey has like 400 million in VC funding | 14:22 |
@kanzure | sounds familiar | 14:22 |
klafka | idk it's still pretty crazy to me | 14:22 |
nmz787 | kanzure: we came up with that library and nicking enzyme idea... just a thought | 14:22 |
@kanzure | nothing here indicates that they are using a nicking enzyme | 14:23 |
@kanzure | and we did not invent the concept of dna libraries -_- | 14:23 |
@kanzure | furthermore, we don't have a patent on that concept either. | 14:23 |
nmz787 | kanzure: just a thought | 14:24 |
nmz787 | it was supposed to be a fun thought | 14:24 |
nmz787 | their progress to date and exit strategy is interesting | 14:24 |
nmz787 | well planned out | 14:25 |
@kanzure | their progress is just some software and fundraising | 14:25 |
nmz787 | by the sound of it | 14:25 |
nmz787 | yeah that's kinda weird | 14:25 |
@kanzure | "make an exit to a specific company" is not "well planned out" dude | 14:25 |
nmz787 | it's not clear when the autoclone software becomes useful | 14:25 |
@kanzure | that's "wishful thinking". anybody can say "and then google will buy us for $100 million." | 14:25 |
nmz787 | i mean their estimates | 14:25 |
nmz787 | it doesn't say a specific company | 14:26 |
nmz787 | spelling error on this page jumps out http://www.deskgen.com/get-involved/ | 14:28 |
nmz787 | 'Enrol' | 14:28 |
@kanzure | "ynthBots communicate with each other and computers over a network, sending and receiving DNA data like a fax machine so that collaborators will always have the latest version of your DNA." | 14:45 |
@kanzure | comparing your product to a fax machine seems sort of archaic.. | 14:45 |
@kanzure | anyway, as far as i can tell, they don't have a prototype. | 14:46 |
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ParahSailin | lol pay someone, living in london no less, 35k for lead X | 14:51 |
@kanzure | for on-site work. | 14:51 |
@kanzure | london is notorious for underpaying their programmers | 14:51 |
@kanzure | but this is a big stretch. | 14:51 |
ParahSailin | or is that 0-10% implying equity? | 14:51 |
@kanzure | yes that implies negotiable equity | 14:52 |
ParahSailin | still, london is fucking expensive to live in | 14:52 |
ParahSailin | i'd be losing money with that pretax salary there | 14:52 |
@kanzure | i think salaries in the UK might be described in post-tax terms, but i'm not sure. | 14:52 |
@kanzure | still a bad deal though. if they think paying someone $35k is good enough for their startup then they must not have a good sense of value. | 14:53 |
klafka | fuck that | 14:58 |
klafka | 35k pounds? | 14:58 |
@kanzure | nmz787: hah, do you remember this? https://groups.google.com/forum/?fromgroups#!topic/diybio/nMGVN_xve-E | 15:09 |
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@kanzure | nmz787: where is your evidence that they raised $7.5M? | 15:19 |
@kanzure | they got $7,500 from a business plan competition, and another $10-15k from an incubator. | 15:20 |
klafka | that's like...nothing | 15:20 |
@kanzure | yes. that's not $7.5M. | 15:20 |
@kanzure | "The British company is currently beta testing the software and expects to be able to launch the SynthBot for construction by 2015." | 15:33 |
@kanzure | from http://mobihealthnews.com/19957/healthbox-london-holds-demo-day-irish-accelerator-healthxl-launches/ | 15:33 |
@kanzure | what does that even mean o_o you don't "launch" a product by building the first prototype.. | 15:35 |
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nmz787_ | Kanzure you're right it was 7500 not 6 | 16:36 |
nmz787_ | Not 7.5 mil | 16:36 |
nmz787_ | * | 16:36 |
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curtiss | https://en.wikipedia.org/wiki/File:Cloned_mice_with_different_DNA_methylation.png | 18:49 |
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@kanzure | this seems unreasonably expensive: | 19:55 |
@kanzure | http://www.ebay.com/itm/BAUM-Humanware-Brailliant-32-portable-bluetooth-USB-Braille-display-/170933827402 | 19:55 |
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phm | Curious. Why are you looking at it? | 19:59 |
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sylph_mako_origi | It could be useful for a wearable. Possibly. | 20:21 |
sylph_mako_origi | If you explicitly wanted to avoid having to use a HMD. | 20:22 |
superkuh | I have a friend that managed to get one of those humanware setups secondhand; would've been $4k normally. | 20:22 |
@kanzure | how much did he pay for his secondhand unit? | 20:22 |
superkuh | I think it was free. | 20:22 |
superkuh | But they're pretty much blind. | 20:23 |
@kanzure | theoretically the reason it's so expensive is because there's not enough blind people that would buy one, so the price has to be great enough to subsidize whatever sillyheaded practices the business making them is conducting. | 20:24 |
@kanzure | i can't imagine the individual braille display units being expensive themselves | 20:24 |
superkuh | Apparently the old models like the one in the ebay link have horrible bluetooth implementations. The new $4k-$6k ones work properly. | 20:30 |
@kanzure | why are there so few characters provided? | 20:37 |
@kanzure | why not 100+ braille characters at a time? | 20:37 |
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@kanzure | secret prior art http://news.ycombinator.com/item?id=5211559 | 21:57 |
@kanzure | was FEI the original OEM of electron microscopes? | 22:23 |
@kanzure | nope, nevermind. | 22:28 |
@kanzure | "Theodor Svedberg invented the analytical ultracentrifuge in 1925,[1] and won the Nobel Prize in Chemistry in 1926 for his research on colloids and proteins using the ultracentrifuge." | 22:31 |
@kanzure | "Ernst Ruska at the University of Berlin, along with Max Knoll, combined these characteristics and built the first transmission electron microscope (TEM) in 1931, for which Ruska was awarded the Nobel Prize for Physics in 1986." | 22:31 |
@kanzure | i think there's an "equipment bias" in nobel prizes :P | 22:32 |
@kanzure | " Instead, what Bardeen, Brattain, and Shockley invented in 1947 was the first bipolar point-contact transistor.[6] In acknowledgement of this accomplishment, Shockley, Bardeen, and Brattain were jointly awarded the 1956 Nobel Prize in Physics "for their researches on semiconductors and their discovery of the transistor effect."[11]" | 22:33 |
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@kanzure | "SciQuest.com(TM), Inc. (Nasdaq: SQST), a leading business-to-business e-marketplace for the global scientific products industry, announced today the launch of a cutting-edge laboratory equipment leasing program via the SciQuest.com Auctions site." | 23:13 |
@kanzure | "Under the terms of this three year partnership, Vista Capital will contribute the resources to pre-approve SciQuest.com Auction users for leases of up to $75,000 with no financial information required." | 23:14 |
@kanzure | "The dollar value of the line is embedded into the software so that each company registrant can see the remaining line of credit in real-time." | 23:14 |
@kanzure | http://www.thefreelibrary.com/SciQuest.com+Partners+with+Vista+Capital+Corporation+to+Create...-a060057276 | 23:15 |
@kanzure | reading that because of this, | 23:15 |
@kanzure | http://iubio.bio.indiana.edu:7131/bionet/mm/cellbiol/1998-June/008901.html | 23:15 |
@kanzure | (this company was acquired by sciquest i think) | 23:15 |
@kanzure | pfft they were an ibm poster-child ftp://ftp.support.lotus.com/software/solutions/pdfs/g325-6614-01.pdf | 23:18 |
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@kanzure | "synthetic genomics' main line of business right now is licensing out gibson assembly" | 23:38 |
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@kanzure | "In 1995, upon Andre Marion retirement, Mike Hunkapiller became President of PE Applied Biosystems Division which sold its 30,000th thermal cycler." | 23:55 |
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