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cerillio | Hi | 00:17 |
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BioGuy | kanzure me? sorry I was looking at other stuff | 00:20 |
Lemminkainen | hallo cerillio | 00:20 |
cerillio | Hi lemmi | 00:21 |
Lemminkainen | wie geht's in your hood? | 00:27 |
BioGuy | Anyone know if there are any federal laws a DIYBio community should know about regarding genetic engineering? | 00:28 |
cerillio | Tir | 00:28 |
cerillio | Tired | 00:28 |
Lemminkainen | BioGuy don't recombine toxin genes in knockout toxigenic bacteria | 00:29 |
BioGuy | Do you have a reference for the law... and any others anyone can think of | 00:30 |
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kanzure | BioGuy: mostly there are zoning restrictions that you should double check | 00:41 |
Lemminkainen | BioGuy the reggs I refer to are anti-bio-terrorism statutes | 00:41 |
Lemminkainen | don't use anything that approaches pathogenic and you'll be fine | 00:42 |
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kanzure | well, there are ways to satisfy biosafety level requirements. | 00:45 |
BioGuy | The guy who started the group asked me when engineering organisms if there's any federal laws we should be aware of. My first instinct told me that this is so new I didn't think there were any... but now I'm second guessing myself. @Lemminkainen ya right now I think there will be a consensus that for all intents and purposes were going to be BSL-1 | 00:45 |
kanzure | for instance, waste disposal is necessary for bsl 1 i think. | 00:45 |
kanzure | which usually means "hire some truck to pick up a colorful bin once in a while" | 00:45 |
Lemminkainen | aye, waste disposal necessary | 00:45 |
Lemminkainen | specific genetic engineering laws don't really come into play until BSL2 | 00:46 |
kanzure | no, your gut instinct is basically wrong... nothing about this is new. | 00:46 |
kanzure | there are local and state laws that you should know about | 00:46 |
BioGuy | You all know where I can get more information on chemical and biological wastes. I'm wondering if my best bet would be just to go down to city hall and ask them. | 00:47 |
Lemminkainen | nooooo | 00:47 |
kanzure | yes you should get to know city hall | 00:47 |
kanzure | Lemminkainen: your advice sucks | 00:47 |
kanzure | BioGuy: read these, http://diyhpl.us/wiki/transcripts/fbi-diybio-2012/ | 00:47 |
Lemminkainen | yeah kanzure let's have BioGuy go down to city hall and hope that the clerk is really receptive to the idea of unlicensed lab space opening | 00:48 |
kanzure | Lemminkainen: operating as a business entity carries with it some requirements, like complying with law. | 00:48 |
Lemminkainen | far better to have BioGuy get some education about this issue BEFORE talkign to city hall | 00:48 |
kanzure | no, he said bsl-1 which usually includes registration/licensing of some sort. | 00:48 |
Lemminkainen | BioGuy you got a local university that does any research? | 00:50 |
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BioGuy | Ya thats another thing I need to look into - see how you can get licensed or register as a BSL-1. Its easy enough to find the CDC guidelines for BSL-1 labs, but I'm having trouble finding who you might go through to be certified. | 00:52 |
BioGuy | Im thinking about going down to the small business development center too and see what they can help us out with. | 00:53 |
BioGuy | *too | 00:53 |
Lemminkainen | ok, BioGuy we're running off on tangents here | 00:53 |
Lemminkainen | let's back u | 00:53 |
Lemminkainen | p | 00:53 |
Lemminkainen | what are you looking to do, exactly? | 00:54 |
kanzure | BioGuy: be sure to ask genspace/biocurious for advice | 00:55 |
BioGuy | Basically provide a space for decentralized and open independent research, development and technology in biology and biotechnology, while meeting federal, state and local regulations | 00:55 |
Lemminkainen | good good | 00:59 |
Lemminkainen | your local university's OSEH people will know a lot and it would be valuable to befriend them | 01:00 |
BioGuy | OSEH good to know, thanks for the tips. I've been thinking about trying to get in touch with the instructor from my college who taught our chemical and biological safety course... though I'm afraid of showing him the current space we have to work with Ha! | 01:07 |
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BioGuy | Kanzure what exactly are these: http://diyhpl.us/wiki/transcripts/fbi-diybio-2012/ They look like transcripts from discussions at hackerspaces maybe? | 01:20 |
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kanzure | BioGuy: i typed these while at the fbi | 01:43 |
NilsHitze | ^^ | 01:49 |
BioGuy | Oh... wait I think I may have heard of something about that. Was there something in 2012 where the FBI invited a bunch of DIYBio organizations to talk about what they were doing? | 01:50 |
kanzure | yes. they also invited non-institutionally-affiliated individuals, such as myself. | 01:52 |
BioGuy | How was it? Was it a conference that went on for several days? | 01:55 |
kanzure | it was fine. i typed those transcripts as they were happening, so there are lots of typos that should be forgiven.. | 01:55 |
gnusha | https://secure.diyhpl.us/cgit/diyhpluswiki/commit/?id=3a507f59 Bryan Bishop: add berlin contact | 01:56 |
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BioGuy | No worries I'm glad you did it, its a good resource. | 02:05 |
kanzure | there was also a similar event held in 2011 but electronic devices were not permitted. | 02:09 |
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* kanzure sleeps | 02:25 | |
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-!- Topic for ##hplusroadmap: biohacking, nootropics, transhumanism, open hardware | sponsored by george church and the NRA | http://gnusha.org/logs http://diyhpl.us/wiki http://groups.google.com/group/diybio | banned by the Federal Death Administration | official paperbot fan club | 04:23 | |
-!- Topic set by kanzure [~kanzure@131.252.130.248] [Sat Mar 23 20:40:45 2013] | 04:23 | |
[Users ##hplusroadmap] | 04:23 | |
[@fenn ] [ chido ] [ He||eshin ] [ nsh ] [ rigel ] [ ThomasEgi ] | 04:23 | |
[ _Sol_ ] [ chris_99 ] [ indigenous ] [ nuba ] [ Sanky ] [ Thorbinator] | 04:23 | |
[ abetusk ] [ Coornail ] [ ivan` ] [ OldCoder_ ] [ saurik ] [ undersco2 ] | 04:23 | |
[ AlonzoTG] [ curtiss ] [ jrayhawk ] [ pad2 ] [ Shehrazad ] [ Urchin ] | 04:23 | |
[ archels ] [ DonnchaC ] [ juri_ ] [ paperbot ] [ sivoais ] [ Viper168 ] | 04:23 | |
[ audy ] [ eleitl ] [ kanzure ] [ ParahSail1n ] [ smeaaagle ] [ yoleaux ] | 04:23 | |
[ augur ] [ ezrios ] [ Lemminkainen] [ ParahSailin ] [ strages_home] [ zubaz ] | 04:23 | |
[ balrog ] [ gedankenstuecke] [ lichen ] [ pasky_ ] [ strangewarp ] | 04:23 | |
[ baslisks] [ gnusha ] [ mutagen ] [ phryk ] [ streety ] | 04:23 | |
[ BioGuy ] [ gnusha_ ] [ NeuroWinter ] [ plur ] [ superkuh ] | 04:23 | |
[ bkero ] [ heath ] [ NilsHitze ] [ qu-bit ] [ Swordsman ] | 04:23 | |
[ brownies] [ HEx1 ] [ nmz787 ] [ randallagordon] [ Thomas42_ ] | 04:23 | |
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ParahSailin | complying with laws is for germans | 08:16 |
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ThomasEgi | actually.. it is impossible for germans to comply with laws | 09:06 |
ThomasEgi | we have so many, that no matter what you do.. you break them. | 09:06 |
ThomasEgi | even if you do nothing because sometimes laws are contradicting. so either you break the one, or the other, but not breaking any is flat out impossible | 09:07 |
eleitl | the Gedankenpolizei would want to have a word with you, mein Herr. | 09:14 |
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EnLilaSko | paperbot: http://link.springer.com/article/10.1023%2FA%3A1021786431815?LI=true | 09:47 |
paperbot | error: HTTP 500 http://diyhpl.us/~bryan/papers2/paperbot/CASE%20REPORT%3A%20Intestinal%20Malabsorption%20as%20a%20Manifestation%20of%20Obstructive%20Sleep%20Apnea.pdf | 09:47 |
jrayhawk | ThomasEgi: http://www.harveysilverglate.com/Books/ThreeFeloniesaDay.aspx the U.S. has the same problem, at least | 09:51 |
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ParahSailin | "Did you really think we want those laws observed? said Dr. Ferris. We want them to be broken. ...The only power any government has is the power to crack down on criminals. Well, when there aren't enough criminals one makes them. One declares so many things to be a crime that it becomes impossible for men to live without breaking laws." | 10:07 |
ParahSailin | also, above i did not intend to imply that current germans are like historical germans | 10:08 |
ThomasEgi | in terms of laws, they are ways worse today | 10:17 |
eleitl | I'm off. See you Tuesday. | 10:26 |
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kanzure | "By linking a number of different datasets that had not previously been used by researchers, Williams was able to measure when genes were sequenced, which genes were held by Celera's intellectual property, and what subsequent investments were made in scientific research and product development on each gene. Williams' conclusion points to a persistent 20-30 percent reduction in subsequent scientific research and product development for those ... | 11:05 |
kanzure | ... genes held by Celera's intellectual property." | 11:05 |
kanzure | http://observationalepidemiology.blogspot.com/2013/03/intellectual-property.html | 11:05 |
kanzure | naturally, we all understand that celera is evil because venter tried to patent the human genome | 11:06 |
brownies | heh, he did? | 11:13 |
brownies | didn't anyone point out there was some prior work? | 11:13 |
kanzure | there was not prior work. celera was the only company trying to sequence the human genome. | 11:13 |
kanzure | later the federal government became interested | 11:14 |
kanzure | and then we got into the human genome project race | 11:14 |
brownies | it was a subtle joke, wrapped around my scintillating insight that there's a difference between "discovering" and "inventing" | 11:14 |
brownies | wouldn't it have made more sense to just patent the appraoches they used to sequence it? | 11:15 |
kanzure | btw my new plan for my caching problem is to have a worker strip GET params, check if the content is the same as the first time/this time, then add the url to a "always strip GET params off of these urls" table to avoid future repeat http requests. | 11:15 |
kanzure | no, because other people had patented that ;) | 11:15 |
brownies | haha | 11:15 |
brownies | kanzure: seems like a simple enough approach | 11:16 |
kanzure | celera had this massive web application back in 1995 or something where you could log in and get "super secret human genome access" or something | 11:16 |
kanzure | of course, you had to pay billions | 11:16 |
brownies | heh | 11:17 |
ParahSailin | celera was not the first group to be sequencing human genome, but they were the first company | 11:18 |
kanzure | right | 11:18 |
EnLilaSko | paperbot: http://www.ajo.com/article/S0002-9394%2804%2901488-6/abstract | 11:33 |
paperbot | no translator available, raw dump: http://diyhpl.us/~bryan/papers2/paperbot/fe646e4a82cd869e7804b48af566310e.txt | 11:33 |
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kanzure | hmm http://www.whitehouse.gov/blog/2013/03/27/white-house-hangout-maker-movement | 11:50 |
kanzure | http://www.cas.org/news/media-releases/springer-and-cas-collaborate | 12:06 |
kanzure | springer is dumping some data into cas' system i guess. | 12:06 |
kanzure | "Springer and Chemical Abstracts Service (CAS) - the world’s authority for chemical information - will collaborate to include thousands of new experimental procedures for chemical reactions reported in Springer journals in the CAS databases. This collaboration will increase the visibility of articles with experimental procedures published in 165 Springer chemistry journals from 1985 to the present" | 12:06 |
kanzure | that was via Peter Carlton <pcarlton@cas.org> | 12:06 |
kanzure | "AAAS moderator: Adam Ruben, scientist-comedian" there's another science comedian? | 12:11 |
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brownies | there shouldn't be any. science is serious business. | 12:18 |
kanzure | there was another one named brian malow | 12:19 |
kanzure | paperbot: http://jeb.biologists.org/lookup/doi/10.1242/jeb.083931 | 12:19 |
paperbot | no translator available, raw dump: http://diyhpl.us/~bryan/papers2/paperbot/Exposure%20to%20multiple%20cholinergic%20pesticides%20impairs%20olfactory%20learning%20and%20memory%20in%20honeybees.pdf | 12:19 |
kanzure | so, is this the main cause of the drop in the bee population? | 12:19 |
kanzure | you could start raising bees with lower-than-environmental doses of the poison in their environment and just breed them until they have a higher tolerance (assuming that you have a sufficiently large population and that there are variations in receptors or whatever the hell bees have for an immune system if anything) | 12:21 |
kanzure | http://www.bbc.co.uk/news/science-environment-21958547 | 12:21 |
jrayhawk | http://www.ncbi.nlm.nih.gov/pmc/articles/PMC2950847/ was a somewhat more convincing explanation | 12:29 |
kanzure | paperbot: http://www.plosone.org/article/info%3Adoi%2F10.1371%2Fjournal.pone.0013181 | 12:29 |
paperbot | error: HTTP 500 http://diyhpl.us/~bryan/papers2/paperbot/Iridovirus%20and%20Microsporidian%20Linked%20to%20Honey%20Bee%20Colony%20Decline.pdf | 12:30 |
jrayhawk | i guess they're not exclusive | 12:30 |
kanzure | i wasn't aware of an rna virus at play in bumblebees | 12:31 |
ParahSailin | that fungicide they put on seed is responsible for colony collapse | 12:36 |
ParahSailin | er, no, the neonicotinoids | 12:37 |
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fenn | i heard it was some kind of fungus | 12:45 |
ParahSailin | lol bromenshank (first author) is a known bayer shill | 12:46 |
ParahSailin | http://money.cnn.com/2010/10/08/news/honey_bees_ny_times.fortune/index.htm | 12:46 |
ParahSailin | fenn: no, it came out a year or two ago that it was clothianidin and thiamethoxam which are commonly coated on corn seeds | 12:48 |
fenn | why would bees ever come into contact with corn seeds | 12:48 |
jrayhawk | oh, interesting | 12:49 |
ParahSailin | thats not the only places those pesticides are used | 12:49 |
ParahSailin | maybe plants uptake the pesticides and make it available to bees in nectar too | 12:50 |
ParahSailin | paperbot: http://www.sciencemag.org/content/336/6079/351 | 12:51 |
paperbot | http://diyhpl.us/~bryan/papers2/paperbot/Neonicotinoid%20Pesticide%20Reduces%20Bumble%20Bee%20Colony%20Growth%20and%20Queen%20Production.pdf | 12:51 |
ParahSailin | this was the "big paper" | 12:51 |
ParahSailin | sometimes i feel like i'm the only one with long-ranging memory | 12:54 |
ParahSailin | other than kanzure | 12:54 |
chris_99 | can anyone recommend any chemistry textbooks? | 13:16 |
ParahSailin | organic, physical, high school? | 13:17 |
chris_99 | is there any that cover bother the former | 13:18 |
ParahSailin | those are gonna be separate books | 13:21 |
chris_99 | hmm how about something like http://www.amazon.co.uk/Chemistry%C2%B3-Introducing-inorganic-physical-chemistry/dp/0199277893/ref=sr_1_1?s=books&ie=UTF8&qid=1364502084&sr=1-1 | 13:21 |
ParahSailin | opencourseware or torrents | 13:23 |
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kanzure | gnome-paint is unusable | 14:20 |
bkero | tuxpaint! | 14:22 |
kanzure | i always end up using kolourpaint.. thought i would try something new. nope. bad idea. | 14:22 |
jrayhawk | fenn: http://toxsci.oxfordjournals.org/content/114/1/1.full a review that agrees with your assessment of BPA research | 14:41 |
ParahSailin | paperbot: http://pubs.acs.org/doi/pdf/10.1021/jf010028%2B | 14:44 |
paperbot | no translator available, raw dump: http://diyhpl.us/~bryan/papers2/paperbot/7a7ce44c21d597f927734b21124b9ad9.pdf | 14:44 |
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ParahSailin | paperbot: http://www.jstor.org/discover/10.2307/362448?uid=2&uid=4&sid=21101940816391 | 15:24 |
paperbot | no translator available, raw dump: http://diyhpl.us/~bryan/papers2/paperbot/The%20New%20England%20Origins%20of%20Mormonism.pdf | 15:24 |
DMXRoid | paperbot: http://www.jstor.org/discover/10.2307/1991070?uid=3739840&uid=2134&uid=2&uid=70&uid=4&uid=3739256&sid=21101834034403 | 15:25 |
paperbot | no translator available, raw dump: http://diyhpl.us/~bryan/papers2/paperbot/On%20a%20Correct%20Measure%20of%20Inflation.pdf | 15:25 |
ParahSailin | kanzure: watermarks detected "This content downloaded from 131.252.130.248 on Thu, 28 Mar 2013 18:24:33 PM" | 15:25 |
DMXRoid | fucking tits | 15:25 |
kanzure | ParahSailin: file a bug report, https://github.com/kanzure/paperbot/issues | 15:25 |
kanzure | oops wait | 15:25 |
kanzure | https://github.com/kanzure/pdfparanoia/issues | 15:25 |
ThomasEgi | DMXRoid, ... that results in a strange mental image. | 15:26 |
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DMXRoid | ThomasEgi: depends on whether tits is the subject or the object | 15:26 |
-!- mode/##hplusroadmap [+o fenn] by kanzure | 15:26 | |
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DMXRoid | i <3 you guys though | 15:27 |
-!- mode/##hplusroadmap [+o Juul] by kanzure | 15:27 | |
@kanzure | might be gone for a bit, please proceed to do my evil bidding for me | 15:28 |
@ParahSailin | go to where? | 15:28 |
@fenn | "hundreds of millions of dollars wasted on BPA research" is sort of hard to believe | 15:35 |
@fenn | jrayhawk: i never claimed any position on BPA besides "gee this is confusing" | 15:35 |
@ParahSailin | anything that gets people to throw out old plastic stuff and buy new plastic stuff is a good market to research | 15:35 |
@fenn | and that it wasn't a transhumanist topic | 15:35 |
@kanzure | ParahSailin: there's this "austin hackers anonymous" event tonight so i thought i'd go and present some things | 15:37 |
@fenn | do they have a 12 step recovery plan | 15:38 |
@kanzure | god i hope so | 15:38 |
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brownies | "My name is Brownies, and I used to write PHP" | 16:29 |
* brownies sobs | 16:29 | |
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* nsh kicks brownies while she's down. SHAME HARDER | 17:54 | |
* nsh comforts brownies in a cynical ploy to fasten the onset of stockholm syndrome | 17:54 | |
* nsh reduces soap rations | 17:54 | |
brownies | she? | 17:55 |
brownies | calm down there, champ | 17:55 |
nsh | well, i tend to use 'he' on the basis of no-women-on-the-internet, but demographics are evening out and i thought, hey, if i'm going to assume anyone on irc is a woman, i'm going to pick the person crying to themselves about coding PHP | 17:57 |
nsh | nah, i'm just foolin' | 17:58 |
@kanzure | ouch | 18:05 |
brownies | you've all done it | 18:17 |
brownies | you've all written PHP and then regretted it | 18:17 |
@kanzure | "dongle.. can we say dongle anymore? does anyone feel uncomfortable?" | 18:18 |
@kanzure | "me, i feel sorta uncomfortable" | 18:18 |
@kanzure | "well it's okay because you're fired" | 18:18 |
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@kanzure | ok back. | 19:43 |
@kanzure | akamai has "silence requirements" https://news.ycombinator.com/item?id=5457214 | 20:58 |
brownies | perhaps just an inept way to say "NDAs" ? | 21:18 |
@kanzure | most of the NDAs i've seen don't say "you can never admit you signed this NDA" | 21:21 |
@kanzure | http://www.phreedom.org/presentations/reverse-engineering-and-security/reverse-engineering-and-security.pdf | 21:28 |
@kanzure | that phreedom seems to be alexander sotirov | 21:28 |
@kanzure | i think our phreedom was evgeny egorochkin | 21:28 |
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@kanzure | http://openwetware.org/index.php?title=User:Jonathan_Cline/Notebook/Sensomatic/20130323&action=render#%281%29 | 23:19 |
@kanzure | http://openwetware.org/index.php?title=User:Jonathan_Cline/Notebook/Sensomatic/20130323 | 23:19 |
@kanzure | "I gave a short presentation at the Carlsbad bio-incubator tonight that was well received. This project proposal is based on a separate DIYBio presentation and later discussions with Cory Tobin of LA Biohackers. The goal of the sensomatic project is to create a tool which eliminates the bring-up hassles of many microbio projects (culturing related). Cory's real (i.e. perhaps to be published) bio project is used as a proxy for requirements ... | 23:19 |
@kanzure | ... generation of the Sensomatic. Along with the requirements: simplicity, low cost, and built to solve a specific problem." | 23:19 |
yashgaroth | yeah he recorded a presentation on it tonight for the video | 23:19 |
@kanzure | http://wiki.biohackers.la/Nitrogenase_Directed_Evolution | 23:19 |
@kanzure | he seems to still be using yaml for Robotics::Tecan. neat. | 23:21 |
@kanzure | "just say no to xml" man this guy is serious he might be dangerous | 23:22 |
@kanzure | paperbot: http://www.frontiersin.org/Neural_Circuits/10.3389/fncir.2013.00052/abstract | 23:37 |
paperbot | no translator available, raw dump: http://diyhpl.us/~bryan/papers2/paperbot/CASK%20and%20CaMKII%20function%20in%20the%20mushroom%20body%20_%20neurons%20during%20Drosophila%20memory%20formation.pdf | 23:37 |
@kanzure | yashgaroth: what video? | 23:37 |
yashgaroth | the one we're doing for indiegogo and/or microryza | 23:38 |
@kanzure | microryza is presently raising money on wefund i think | 23:38 |
yashgaroth | how deep does the rabbit hole go | 23:38 |
@kanzure | i'm not sure why. i can't think of any potential exits that they might have. even at 50x valuation you'd only earn $50k on that investment (i think it's capped at $1k/person). | 23:39 |
yashgaroth | but surely there's billions in crowdfunding ecologists to go observe [cute animal] and discover why they're going extinct | 23:40 |
@kanzure | even if there is, i don't see how microryza would be valuable based on that. what are they going to do, sell themselves to NIH ? | 23:41 |
@kanzure | i suppose science exchange has a small chance of being purchased by one of the mega chemical companies | 23:41 |
@kanzure | or big pharma somewhere. those guys love stuff about outsourcing to CROs. | 23:42 |
yashgaroth | and microryza is only competing with non-profit/gov't agencies | 23:42 |
@kanzure | i could imagine maybe some bigger non-profit buying them up maybe, if they feel it's important to look more efficient or accessible w/e | 23:43 |
@kanzure | but watsi sort of has that covered already | 23:44 |
yashgaroth | haha wtf someone on microryza wants to use a chemotherapy drug to reduce transmission of the brca mutation | 23:44 |
yashgaroth | "I am currently the co-founder and CEO of Science Exchange, the online marketplace for science experiments. " oh | 23:44 |
@kanzure | link to this one? | 23:45 |
yashgaroth | https://www.microryza.com/projects/can-we-prevent-the-transmission-of-brca-mutations | 23:45 |
@kanzure | is elizabeth the ceo of science exchange? or was that one of hte other ones | 23:45 |
@kanzure | ah... yes. | 23:45 |
@kanzure | hilarious. | 23:45 |
yashgaroth | 'other methods like pre-implantation diagnosis have significant drawbacks' oh yeah unlike taking chemo | 23:46 |
@kanzure | chillax dude, me and my homie were taking some chemo last week and it was grand | 23:48 |
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yashgaroth | yeah man it's great for a nice relaxing weekend of hair loss and vomiting | 23:48 |
@kanzure | don't forget the butt bleeds | 23:48 |
yashgaroth | I try to forget | 23:48 |
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@kanzure | http://blog.reprap.org/2011/03/automatic-documentation-and-project.html | 23:59 |
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