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AshleyWaffle | Hi | 00:14 |
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AshleyWaffle | night, I'll be on tomorrow | 00:14 |
kanzure | http://www.neocomputer.org/projects/et "Fixing E.T. The Extra-Terrestrial for the Atari 2600" | 00:28 |
JayDugger | Seriously? "why do people hate E.T.?" | 00:32 |
JayDugger | Answer: "It was completely non-violent. You can't hurt the bad-guys, and they can't hurt you. There isn't even any competition!" | 00:32 |
JayDugger | I remember playing that game as a child. Boring. | 00:32 |
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chido | kanzure: he's part of my lab :) | 00:46 |
kanzure | excellent | 00:46 |
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kanzure | redsocks really eats up cpu if you let it run forever | 01:03 |
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kanzure | $35/hour for... well it doens't [A[A[A[A | 01:24 |
kanzure | irssi error. bleh. | 01:24 |
nmz787 | http://en.wikipedia.org/wiki/List_of_animals_by_number_of_neurons | 02:11 |
nmz787 | we're billionaires! | 02:13 |
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pasky | http://www.newscientist.com/article/mg21829114.700-hacked-medical-scanner-becomes-a-nuclear-forge.html - seems like the mainstream media notion of "hacking" is shifting in the good direction :) | 04:37 |
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kanzure | paperbot: http://www.plosone.org/article/info:doi/10.1371/journal.pone.0060410 | 09:12 |
paperbot | error: HTTP 500 http://diyhpl.us/~bryan/papers2/paperbot/Non-Invasive%20Brain-to-Brain%20Interface%20%28BBI%29%3A%20Establishing%20Functional%20Links%20between%20Two%20Brains.pdf | 09:12 |
kanzure | "Non-Invasive Brain-to-Brain Interface (BBI): Establishing Functional Links between Two Brains" | 09:12 |
kanzure | "Transcranial focused ultrasound (FUS) is capable of modulating the neural activity of specific brain regions, with a potential role as a non-invasive computer-to-brain interface (CBI). In conjunction with the use of brain-to-computer interface (BCI) techniques that translate brain function to generate computer commands, we investigated the feasibility of using the FUS-based CBI to non-invasively establish a functional link between the brains ... | 09:12 |
kanzure | ... of different species (i.e. human and Sprague-Dawley rat), thus creating a brain-to-brain interface (BBI). The implementation was aimed to non-invasively translate the human volunteer’s intention to stimulate a rat’s brain motor area that is responsible for the tail movement. The volunteer initiated the intention by looking at a strobe light flicker on a computer display, and the degree of synchronization in the electroencephalographic ... | 09:12 |
kanzure | ... steady-state-visual-evoked-potentials (SSVEP) with respect to the strobe frequency was analyzed using a computer. Increased signal amplitude in the SSVEP, indicating the volunteer’s intention, triggered the delivery of a burst-mode FUS (350 kHz ultrasound frequency, tone burst duration of 0.5 ms, pulse repetition frequency of 1 kHz, given for 300 msec duration) to excite the motor area of an anesthetized rat transcranially. The ... | 09:13 |
kanzure | ... successful excitation subsequently elicited the tail movement, which was detected by a motion sensor. The interface was achieved at 94.0±3.0% accuracy, with a time delay of 1.59±1.07 sec from the thought-initiation to the creation of the tail movement. Our results demonstrate the feasibility of a computer-mediated BBI that links central neural functions between two biological entities, which may confer unexplored opportunities in the ... | 09:13 |
kanzure | ... study of neuroscience with potential implications for therapeutic applications." | 09:13 |
kanzure | 1.59 seconds, geeze. | 09:14 |
FooQuuxman | Been waiting for non-invasive Mind-Machine interfaces for a while | 09:23 |
FooQuuxman | good to know they are starting to show up | 09:23 |
kanzure | it said nothing about minds, what the fuck dude | 09:25 |
* kanzure finds lunch | 09:26 | |
FooQuuxman | sorry, wtf? | 09:26 |
kanzure | read it again. it says nothing about minds. | 09:26 |
FooQuuxman | Brain, Mind, whats the difference? | 09:26 |
kanzure | tremendous. | 09:26 |
FooQuuxman | or better way to put it: what is the difference as far as pluging in goes? | 09:28 |
kanzure | studying one of them will lead to actionable results | 09:29 |
kanzure | the other will not | 09:29 |
abetusk | one is the organ, the other is what the organ does | 09:29 |
kanzure | not really | 09:29 |
abetusk | unless you're a dualist | 09:30 |
FooQuuxman | oh I see, that makes more sense | 09:30 |
kanzure | i am not a dualist, i just believe philosophers tend to suck | 09:30 |
FooQuuxman | *tend* to? | 09:30 |
kanzure | and that philosophy is no replacement for actually poking at the brain | 09:30 |
abetusk | you'll get no argument from me | 09:30 |
kanzure | also the other part of your comment that i found objectionable was the part about waiting | 09:32 |
kanzure | perfect storm of terrible things | 09:33 |
AshleyWaffle | kanzure: I agree about the waiting thing. But it's pretty clear he meant brain | 09:53 |
AshleyWaffle | I don't think you'll find any religious nuts here | 09:53 |
AshleyWaffle | So don't pretend that's the case | 09:53 |
AshleyWaffle | although... isn't FooQuuxman catholic or something? | 09:53 |
FooQuuxman | catholic, no | 09:54 |
AshleyWaffle | Are you religious? | 09:54 |
FooQuuxman | yes | 09:54 |
FooQuuxman | I would be considered a dualist, but the 'line' between mind and brain is currently too blury to be useful | 09:55 |
FooQuuxman | I could even be convinced that the mind is just software | 09:55 |
AshleyWaffle | FooQuuxman: Well, in a sense that's true I guess. A brain could just as easily be a random and meaningless neural network that does nothing. The configuration of neurons does matter a lot and I guess could be considered "software" | 09:57 |
AshleyWaffle | But I think we're all focused on the configuration aspect - computers can already do small neural network simulations | 09:57 |
ParahSail1n | ghoooooooooooossts | 09:57 |
FooQuuxman | woooooooooooo | 09:58 |
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kanzure | see! i spotted the dualist from just a single comment. | 10:22 |
FooQuuxman | woot! how many internets is that worth? | 10:22 |
kanzure | one. why are you here? | 10:23 |
FooQuuxman | because people on #mises kept saying that if someone wanted ideas for projects to come here | 10:24 |
FooQuuxman | I was here to look around and figure out the channel | 10:25 |
kanzure | what is #mises? | 10:25 |
FooQuuxman | as in ludwig von mises | 10:25 |
kanzure | i'm not seing the connection.. why would they tell you to come here? | 10:30 |
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FooQuuxman | nah, they weren't telling me, it was someone else | 10:31 |
FooQuuxman | Kelvin maybe? | 10:31 |
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kanzure | what sort of projects? | 10:39 |
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kanzure | paperbot: http://www.nature.com/ng/journal/v45/n4/full/ng.2528.html | 11:40 |
paperbot | http://diyhpl.us/~bryan/papers2/paperbot/Identification%20of%20seven%20loci%20affecting%20mean%20telomere%20length%20and%20their%20association%20with%20disease.pdf | 11:40 |
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kanzure | "Also, at the cheapest how much would a PCR reaction be (with and without polymerase)? Any thoughts?" | 16:22 |
kanzure | i think that goes away if you exercise more | 16:22 |
kanzure | oops | 16:22 |
kanzure | why would it be PCR if you have no polymerase | 16:22 |
brownies | just a regular ol' chain reaction | 16:23 |
ParahSail1n | maybe he means cost including and not including enzyme? | 16:24 |
ParahSail1n | you should ban mega from diybio mailing list | 16:28 |
kanzure | i am not a moderator | 16:28 |
kanzure | in fact i'm pretty sure they've considered banning me often | 16:28 |
ParahSail1n | oh is cathal moderator? | 16:28 |
kanzure | no | 16:28 |
kanzure | only mac cowell and jason bobe | 16:29 |
yashgaroth | man I would ban so many people from the mailing list if I could | 16:30 |
kanzure | kickbans for everyone! | 16:31 |
ParahSail1n | what did you do to piss them off kanzure | 16:31 |
kanzure | i have always been opposed to their ideas for making top-down policy decisions and shit | 16:32 |
ParahSail1n | i didnt know they had any policy decisions | 16:33 |
kanzure | because i kept yelling at them :P | 16:33 |
kanzure | things like, demanding that everyone signs on to a code of ethics | 16:34 |
ParahSail1n | what were they trying to police | 16:34 |
ParahSail1n | ah geez | 16:34 |
brownies | a code of ethics? for a mailing list? | 16:34 |
kanzure | or pretending to represent all of the interests of everyone on the mailing list | 16:34 |
kanzure | yes | 16:34 |
kanzure | they also wanted a moratorium on biohacking projects at one point | 16:34 |
ParahSail1n | fucking busybodies should just get busy | 16:34 |
ParahSail1n | diybio:biohacking::siai:ai? | 16:35 |
kanzure | jason gets grants from woodrow wilson foundation to "study" diybio | 16:35 |
brownies | "Thou shalt not suffer a thread to be off-topic" | 16:35 |
kanzure | brownies: nah, more specific biohacking ethics things | 16:35 |
kanzure | i think it is very misleading to claim to government bodies that everyone agrees to some code of ethics | 16:35 |
kanzure | for individual institutions/companies like biocurious and genspace, i think a code of ethics is a grand plan that works out pretty well. | 16:36 |
kanzure | of course, that's not diybio. | 16:36 |
kanzure | or, rather, it might be diybio but it's certainly not going to accurately represent the motivations of independent individuals working on their own biology projects | 16:37 |
kanzure | like cathal. | 16:37 |
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abetusk | um, so what is the cheapest you can source reagents for a PCR experiment? | 17:53 |
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ParahSail1n | assuming you can produce your own pol, dntps are the limiting reagent | 17:56 |
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kanzure | ParahSail1n: i don't think anyone in diybio has produced their own polymerase yet | 18:25 |
ParahSail1n | how that seems pretty incompetent-- i mean, i haven't either, but i haven't been in a wetlab for a while | 18:26 |
abetusk | I thought there was a guy selling taq out of his garage...? | 18:26 |
kanzure | who was selling taq? | 18:26 |
abetusk | Maybe I just have a faulty memory | 18:27 |
yashgaroth | wasn't avery doing something with opentaq | 18:27 |
abetusk | I thought you linked to it at one point, though I would probably have saved that link and I can't find it... | 18:27 |
kanzure | opentaq was what john schloendorn was selling on openbiotech.com | 18:28 |
kanzure | i don't know if john was making his taq himself or not | 18:28 |
ParahSail1n | cathal seems to be someone who does stuff, but he seems scared of patents | 18:28 |
kanzure | john sells a taq expression vector i think | 18:28 |
ParahSail1n | is it broken in some way? | 18:29 |
kanzure | no i think avery claims he got it to work at least once | 18:29 |
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kanzure | i still think a lot of his claims are unsubstantiated | 18:32 |
kanzure | "Ultimately, we anticipate no less than an industry-wide collapse of biological reagent pricing as a direct result of our open source policies. " | 18:32 |
kanzure | on http://www.openbiotech.com/Default.asp | 18:32 |
kanzure | "Open MasterMix is an optimized convenient premixed formulation of Taq polymerase and dNTPs in a buffer solution at 5x concentration for simple dilution when primers and template are added." | 18:34 |
kanzure | well i guess he is selling taq. | 18:34 |
kanzure | damn he is also selling a 293 strain? | 18:35 |
kanzure | i need to get in on this shit | 18:35 |
yashgaroth | and yet somehow not one e.coli strain | 18:36 |
abetusk | pOpenTaq, that's pretty awesome | 18:37 |
ParahSail1n | i can give you 293 cells | 18:39 |
ParahSail1n | wow 100 is not a bad price | 18:40 |
yashgaroth | can you give me some dh5a's? | 18:40 |
ParahSail1n | yashgaroth, i can do you one better | 18:41 |
yashgaroth | what, competent ones? | 18:42 |
ParahSail1n | get free samples http://lucigen.com/store/E.-cloni-10G-and-10GF-Chemically-Competent-Cells/ | 18:42 |
yashgaroth | well they're not relA1- but I can live with that for $free | 18:43 |
ParahSail1n | recA? | 18:43 |
yashgaroth | nah, relA is the relaxed response to amino acid starvation where they still produce plasmid | 18:44 |
ParahSail1n | oh | 18:44 |
ParahSail1n | are 5alphas relA1-? | 18:45 |
yashgaroth | yeah, but honestly it's not a huge problem unless you're just doing overnight cultures, which I hope not to | 18:46 |
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yashgaroth | now to just make up an 'institution' for the request form | 18:47 |
kanzure | you can say you're with blackhatbio.com if you want | 18:48 |
ParahSail1n | put random words together | 18:48 |
yashgaroth | I don't think the local diybio lab is active enough to ship there, but I really doubt lucigen accepts residential addresses | 18:49 |
ParahSail1n | the employee reading the order form is not going to make more or less money depending on whether he vets all the institutions they send samples to | 18:49 |
yashgaroth | university of the house I live in | 18:50 |
yashgaroth | oh god "E. cloni®" | 18:51 |
kanzure | i thought it was a typo in the url | 18:51 |
ParahSail1n | yashgaroth, hey, the shit works | 18:51 |
kanzure | /store/E.-cloni-10G | 18:51 |
ParahSail1n | competent as hell | 18:51 |
yashgaroth | I wonder how well they store at -20 | 18:52 |
yashgaroth | and by -20 I mean whatever my kitchen freezer puts out | 18:52 |
ParahSail1n | oh yeah dont squander them like that | 18:52 |
ParahSail1n | unless you just need a subcloning grade strain for making plasmid | 18:53 |
ParahSail1n | you can do pretty much anything to get e coli to make colonies on a plate | 18:53 |
yashgaroth | well if I had the plasmid ready I could just do the transfection upon receipt, but fuck re-competentizing cells | 18:54 |
ParahSail1n | so you could definitely store them as plate streaks or whatever | 18:54 |
yashgaroth | the local lab's -80 should be working aaaany year now | 18:54 |
ParahSail1n | you can pretty much grow up a batch of cells and pellet them down and mix dna and youll get colonies | 18:55 |
ParahSail1n | competency is for getting cloning to work | 18:55 |
yashgaroth | can you just send me an agar stab of them? assuming that you recommend them from personal experience | 18:56 |
yashgaroth | I would be super appreciative | 18:56 |
ParahSail1n | i dont have access to a lab anymore | 18:57 |
ParahSail1n | they'll give you a sample | 18:58 |
yashgaroth | how long have they been giving samples out for? if it's an ongoing thing I may wait til I have a more legit address | 18:59 |
ParahSail1n | they've "always" given out samples | 19:00 |
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* brownies pokes kanzure | 19:35 | |
brownies | how many bookmarks do you have? | 19:35 |
brownies | does Chrome slow down after some threshold # of bookmarks? | 19:35 |
kanzure | i can't bookmark anymore because chrome crashes | 19:36 |
brownies | i... see. | 19:36 |
brownies | how many bookmarks did that involve? | 19:36 |
brownies | order of magnitude | 19:37 |
kanzure | not many. i have a primary bookmark collection of about 57,000ish. the one in chrome is <5000. | 19:37 |
brownies | yeah i have approximately the same amount in Chrome and it just... gave up on favicons | 19:37 |
brownies | that's a recent development, but i find it odd. | 19:37 |
kanzure | i've been meaning to send a bug report to chrome upstream but since i don't have debug symbols i stopped caring. | 19:38 |
kanzure | i get a segmentation fault when i add a new bookmark | 19:38 |
brownies | kanzure: where the hell do you keep 57K bookmarks? | 19:38 |
kanzure | elaborate file system | 19:38 |
brownies | ah, i can still bookmark. i just get major lag when i try to do so. or if i accidentally mouse over the bookmarks menu in the navbar. | 19:38 |
kanzure | originally my collection began in firefox, then i moved to opera which seemed more able to handle larger numbers of bookmarks. then i switched to this weird flatfile system thing that i'm not 100% happy with yet. | 19:39 |
kanzure | i think way back when my collection in firefox was using its dumb html bookmarking format before it got sqlite bookmarks :( | 19:39 |
ParahSail1n | i hear firefox isnt terrible memory leaker anymore? | 19:40 |
kanzure | firefox has been making improvements in a lot of memory leaking areas but as far as i can tell it still can't handle >400 tabs well. | 19:41 |
ParahSail1n | kinda reluctant to try it and see | 19:41 |
ParahSail1n | heh 400 tabs | 19:41 |
kanzure | i suspect that part of the problem was that firefox was always only 32 bit builds? | 19:41 |
kanzure | i think there might be a 64 bit nightly build | 19:42 |
brownies | eh, firefox's memory-leakage cured me of my desire to run >100 tabs. now i routinely stay under 50. | 19:43 |
brownies | so in a sense... it was self-correcting. | 19:43 |
kanzure | i want a better bookmarking solution | 19:50 |
kanzure | all the online tagging things don't really handle more than a handful of bookmarks well | 19:50 |
kanzure | "pfft only power users will have tens of thousands of bookmarks, and that will be just one guy" | 19:50 |
kanzure | well guess what that's me :( | 19:50 |
brownies | pinboard has been working fine for me | 19:51 |
brownies | i have well over 10K bookmarks | 19:51 |
kanzure | hm i admit i didn't try dumping everything into pinboard.in | 19:52 |
brownies | it's well worth the $10 or whatever he charges | 19:53 |
kanzure | i wanted to try historio.us at one point but it looked like it would break after two or three bookmarks | 19:55 |
brownies | i liked the idea of historious, mostly because i had the exact same idea myself | 19:57 |
brownies | but by that time i had already paid for pinboard, and then the pinboard guy rolled out his archiving feature, so that was that. | 19:57 |
kanzure | thug life. | 19:58 |
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ParahSail1n | paperbot: http://www.jstor.org/stable/41560160 | 20:10 |
paperbot | no translator available, raw dump: http://diyhpl.us/~bryan/papers2/paperbot/f6398794044dc24e9a357ea9f9611625.txt | 20:10 |
rigel | http://shop.lenovo.com/seind/SE/en/learn/products/laptops/thinkpad/thinkpad-edge/s430/index.html | 20:52 |
rigel | so where the hell do i get this laptop in the US | 20:52 |
kanzure | keyboard sucks | 20:56 |
kanzure | http://www.lenovo.com/shop/WW/products/splitter/notebooks/ThinkPad/Edge/gallery/ThinkPad-Edge-S430-Laptop-PC-Close-up-Keyboard-View-5L-940x475.jpg | 20:56 |
kanzure | i recently picked up a thinkpad w520 | 20:58 |
kanzure | which has the non-sucky keyboard and 2x RAM capacity of the S430 | 20:58 |
rigel | thunderbolt | 20:59 |
rigel | thats the only reason to get this laptop | 20:59 |
kanzure | ah. | 20:59 |
rigel | because 1394 is apparently dead on laptops | 20:59 |
rigel | and why the hell hasnt someone come up with a device/driver combo that can use a gigE port, like EVERY GODDAMN MODERN LAPTOP HAS NOW, as though it were e.g. 1394 | 21:00 |
rigel | i mean sure you'd need to have some software to handle it somehow, but that should totally be possible | 21:01 |
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nmz787 | ethernet has a fair amount of overhead | 21:08 |
nmz787 | it's made to be able to pass through swithces and routers | 21:08 |
nmz787 | so it's not as cheap as peripheral protocols can use | 21:09 |
nmz787 | usb3 is pretty fast though | 21:09 |
nmz787 | i don't like that USB can fit into ethernet ports actually | 21:09 |
nmz787 | on my laptop, on the base station the ethernet is next to the power jack, and the power plug can fit into the ethernet port too | 21:10 |
nmz787 | so that's pretty anoyying | 21:10 |
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kanzure | eleitl: alonzo is actually muted in here for those very reasons. | 21:33 |
ParahSail1n | ? | 21:35 |
kanzure | ParahSail1n: alonzotg believes that we are all conspiring to secretly upload him without his knowledge | 21:35 |
kanzure | ParahSail1n: and eugen has been debunking alonzotg's claims for years now | 21:36 |
ParahSail1n | how do you debunk such a claim | 21:36 |
kanzure | well, in particular, i was referring to eugen's recent emails | 21:37 |
ParahSail1n | ah | 21:37 |
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kanzure | also jrayhawk spent some time trying to debug him, i dunno if those logs are public | 21:41 |
ParahSail1n | by upload you mean upload his brain to a computer? | 21:43 |
kanzure | yes. | 21:43 |
kanzure | this thread- http://lists.extropy.org/pipermail/extropy-chat/2013-April/076485.html | 21:44 |
kanzure | more obvious from http://lists.extropy.org/pipermail/extropy-chat/2013-April/thread.html | 21:44 |
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ParahSail1n | and why do you guys take that theory seriously enough to expend energy responding in any way? | 21:45 |
kanzure | entertainment | 21:45 |
ParahSail1n | the mentally ill are always a good source of it | 21:45 |
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* strophariad glances topicward | 22:12 | |
strophariad | for nootropics, consider hericium erinaceus | 22:12 |
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nmz787 | paperbot: http://scitation.aip.org.proxy.lib.pdx.edu/getpdf/servlet/GetPDFServlet?filetype=pdf&id=AJPIAS000078000007000671000001&idtype=cvips&doi=10.1119/1.3427413&prog=normal | 22:17 |
paperbot | no translator available, raw dump: http://diyhpl.us/~bryan/papers2/paperbot/c162878fe64919687fc5b56b7b3553e4.pdf | 22:17 |
nmz787 | paperbot: are you getting old and/or tired? you seem a bit slow today | 22:17 |
yashgaroth | .botstim | 22:18 |
nmz787 | or did it have to fail a ton of if statements? | 22:18 |
brownies | .botsnack | 22:23 |
yoleaux | :D | 22:23 |
kanzure | it has to fetch the target url twice | 22:45 |
kanzure | once with zotero translation-server | 22:46 |
kanzure | and then again with the python fallbacks | 22:46 |
kanzure | my guess is that when zotero translation-server tried to pull up scitation.aip.org.proxy.lib.pdx.edu it was hit with a series of redirects because most ezproxy instances are configured to let internal ip addresses through via http redirect | 22:46 |
kanzure | and then it downloaded the pdf to zotero translation-server, which doesn't know how to handle raw pdf (it only returns metadata to paperbot) | 22:47 |
nmz787 | paperbot: http://www.opticsinfobase.org/ol/abstract.cfm?uri=ol-24-10-670 | 22:50 |
paperbot | http://diyhpl.us/~bryan/papers2/paperbot/5c1d93f8a531d09f81923f84a7abdc56.txt | 22:50 |
kanzure | if you ever want to see something wacky.. | 22:55 |
kanzure | paperbot: http://httpbin.org/get | 22:55 |
paperbot | no translator available, raw dump: http://diyhpl.us/~bryan/papers2/paperbot/fcd8bef2d335baa2c57599bacdaf2c49.txt | 22:55 |
kanzure | there used to be this service similar to httpbin that would show you multiple incoming requests to a target url | 22:55 |
brownies | huh. that is wacky. | 22:55 |
kanzure | there's actually two outgoing requests to httpbin.org/get but obviously only one is going to be showed there | 22:56 |
kanzure | this could be eliminated with a simple caching server if i was not lazy | 22:56 |
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nmz787 | paperbot: http://link.springer.com/article/10.1007%2Fs00542-006-0367-9 | 23:03 |
paperbot | error: HTTP 500 http://diyhpl.us/~bryan/papers2/paperbot/A%20new%20DNA%20chip%20detection%20mechanism%20using%20optical%20pick-up%20actuators.pdf | 23:03 |
nmz787 | paperbot: http://pubs.rsc.org/en/content/articlelanding/2011/lc/c0lc00577k | 23:05 |
paperbot | no translator available, raw dump: http://diyhpl.us/~bryan/papers2/paperbot/Optical%20tweezers%20directed%20one-bead%20one-sequence%20synthesis%20of%20oligonucleotides.pdf | 23:05 |
kanzure | anders will be speaking in stockholm tomorrow (monday) on whole brain emulation http://agenda.albanova.se/conferenceDisplay.py?confId=3904 | 23:19 |
kanzure | archels: i think you're close? maybe? | 23:19 |
archels | too bad, I'm only going next weekend | 23:30 |
kanzure | email him and ask him to delay | 23:31 |
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