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| kanzure | i think i will do some midnight soul searching on c2 http://c2.com/cgi/wiki?ObjectiveEvidenceAgainstGotos | 00:54 |
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| superkuh | http://order.foc.us/ | 00:56 |
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| kanzure | "Make your synapses fire faster." | 00:56 |
| kanzure | um.. | 00:57 |
| kanzure | "The headset passes a small electric current (<2.05mA) through the prefrontal cortex of the wearer." well that's vague. | 00:58 |
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| kanzure | there is a company called 'bioglow' that seems to be doing glowing things :( http://www.aphis.usda.gov/biotechnology/downloads/reg_loi/aphis_response_bioglow_032113.pdf | 08:46 |
| brownies | is this different from the kickstarter guys? | 08:47 |
| kanzure | yes | 08:52 |
| kanzure | pffft "postdocpocalypse" | 08:52 |
| brownies | good. the more companies we have on this, the faster i can get a glowing cat. | 09:21 |
| kanzure | soon you too will hate glowing things | 09:22 |
| kanzure | and your rebels friend are walking into a trap, as is your pathetic fleet. | 09:22 |
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| kanzure | *rebel friends | 09:22 |
| eudoxia | oh shit mike's blog is down http://chronopause.com/ | 09:22 |
| brownies | probably will cost a fortune in cat food. | 09:23 |
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| eudoxia | .meow | 09:34 |
| yoleaux | http://moar.edgecats.net/cats/tumblr_mm5bjfDbG61qdlh1io1_400.gif | 09:34 |
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| chris_99 | paperbot: http://ieeexplore.ieee.org/xpl/login.jsp?tp=&arnumber=6103636&url=http%3A%2F%2Fieeexplore.ieee.org%2Fxpls%2Fabs_all.jsp%3Farnumber%3D6103636 | 10:25 |
| paperbot | no translator available, raw dump: http://diyhpl.us/~bryan/papers2/paperbot/99ecfee2e69401f616cb1373b7755806.txt | 10:25 |
| kanzure | it would help if you try the abs_all link itself instead of the login.jsp link | 10:27 |
| chris_99 | aha | 10:29 |
| chris_99 | paperbot: http://ieeexplore.ieee.org/xpls/abs_all.jsp?arnumber=6103636 | 10:29 |
| paperbot | http://diyhpl.us/~bryan/papers2/paperbot/b04c418d67db99df5aecd89b0299ebdb.txt | 10:29 |
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| oblique | hello guys, what is your opinion about http://foc.us ? isn't too expensive? | 12:28 |
| chris_99 | i bet you could make something similar a lot cheaper | 12:30 |
| kanzure | and their marketing sucks, and wouldn't you prefer something open source, and a few other issues | 12:31 |
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| chris_99 | can you combine EEG + tDCS to see the effect of the stimulation | 12:33 |
| oblique | kanzure: i prefer opensource, but i don't know anything about electrical engineering, i only know programming | 12:33 |
| kanzure | maybe you should fix that? i promise it is worthwhile. | 12:33 |
| kanzure | chris_99: i haven't seen an effective demonstration of that. if you only care about whether or not the eeg sensors light up, i guess that's okay. but you could also just use an oscilloscope.. | 12:34 |
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| oblique | kanzure: are there any good opensource tdcs? openstim is good and safe? | 12:36 |
| kanzure | /home/gnusha/public_html/logs/2012-07-09.log:16:01 < kanzure> openstim was basically just ed boyden throwing some parts together and then never publishing anything, i think | 12:38 |
| kanzure | /home/gnusha/public_html/logs/2008-12-04.log:06:15 < kanzure> openstim is dead | 12:39 |
| oblique | so, any alternatives? | 12:39 |
| biolulz | loving that outdoor chick photoshoot though. Appeal to that "gamer demographic" ?!! | 12:40 |
| kanzure | there was another tdcs marketing stunt from last year that never materialized, it was also proprietary | 12:40 |
| kanzure | if you want things to happen, the best way to proceed is to do it yourself | 12:40 |
| kanzure | http://diyhpl.us/~bryan/papers2/neuro/tdcs/ | 12:40 |
| oblique | thx! | 12:41 |
| oblique | rdb was thinking to build something good, but he | 12:42 |
| oblique | is busy with real life | 12:42 |
| kanzure | so what? | 12:42 |
| oblique | well, someone with good knowledge have to do it | 12:44 |
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| kanzure | "Flickr gets new UI, new Android app, 1TB free space" oh hm, maybe that's where i can store all my things | 14:44 |
| fenn | my hobby is taking pictures of seemingly random noise | 14:45 |
| kanzure | stop criticising my artistic vision | 14:46 |
| kanzure | it is a very very very specific vision | 14:46 |
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| kanzure | unfortunately i don't think gzip streams look random | 14:50 |
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| Mokstar | Whoo! | 15:24 |
| Mokstar | ThomasEgi, So, would you just have a pair of pins, and change the pinmode from output to input really fast? | 15:24 |
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| Mokstar | I wish I had smart :( | 15:27 |
| ThomasEgi | what was it you'r trying to do again? | 15:28 |
| ThomasEgi | anyway. i'm lacking sleep. the best advice i can give you is not to listen to my advice right now^ | 15:32 |
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| Mokstar | Hah :p | 15:34 |
| Mokstar | tRNS | 15:34 |
| Mokstar | but you're gone now | 15:34 |
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| ParahSailin | 1TB is a lot of papers | 15:57 |
| ParahSailin | 1 TB per captcha solution that is | 15:57 |
| kanzure | deathbycaptcha is something like $0.0001/captcha | 16:02 |
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| heath | kanzure: http://diyhpl.us/~bryan/nucleic/fbi-diybio-dna-v1.pdf is the second result for "diy dna synthesizer" in an ingognito mode browser over here | 18:31 |
| kanzure | yep | 18:33 |
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| heath | nmz787: so what's the status on your project? | 20:29 |
| heath | i just finished watching https://www.youtube.com/watch?v=b6zpEyDvdRA | 20:30 |
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| kirka | Hi guys | 22:23 |
| kirka | Hi, kanuzre | 22:23 |
| kirka | kanzure | 22:23 |
| Adifex | sup | 22:23 |
| kanzure | kirka: welcome back. | 22:24 |
| kirka | kanzure Thanks for http://diyhpl.us/~bryan/papers2/bio/ , these are interesting papers | 22:25 |
| kanzure | you might be interested in http://diyhpl.us/~bryan/papers2/longevity/ | 22:25 |
| kanzure | and http://diyhpl.us/~bryan/papers2/microfluidics/ | 22:25 |
| kirka | I have started reading some textbooks on molecular biology some time ago | 22:26 |
| Adifex | by the way, I'm looking for a bio-related project or two on github or sourceforge that could use some help with python. Does anyone have any suggestions? | 22:26 |
| kanzure | biopython | 22:29 |
| kirka | kanzure Don't you know a good book on E.coli? Something that describes current understanding of it's genome functionality. I have found Escherichia coli: virulence mechanisms of a versatile pathogen, but it's not exactly what I want | 22:30 |
| kanzure | the current understanding of its genome is on ncbi's website | 22:31 |
| kirka | It'd be cool to have "genome atlas" of E.Coli where it's proteins and metabolic pathway would be described in detail | 22:32 |
| kirka | Ah, genome annotation | 22:32 |
| Adifex | hm. that looks pretty cool, thanks | 22:32 |
| kirka | kanzure Do you think that biocyc is a good idea? It's propietary though | 22:34 |
| kanzure | i think the other databases are good | 22:34 |
| kirka | I'm reading "Molecular biology of the cell", but it's very general | 22:37 |
| klafka | good book | 22:38 |
| klafka | i read that | 22:38 |
| kirka | It'll take some time to read these 1200+ pages | 22:39 |
| kanzure | you should also read the other books here: http://diyhpl.us/wiki/diybio/faq/books | 22:39 |
| kirka | I have read the SB primer already, it's a good introduction | 22:40 |
| kirka | good list | 22:40 |
| Adifex | who runs the diyhpl.us site? | 22:44 |
| kanzure | i do. | 22:45 |
| Grognor | only death awaits the unprepared https://twitter.com/RachelHaywire/status/336669832734965760 | 22:46 |
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| kanzure | uh.. please don't bring that in here. | 22:47 |
| kanzure | can't help but point out that 169.232.212.23 is los angeles (where rachel lives) | 22:54 |
| kanzure | wasn't this the homeless person? | 22:56 |
| kirka | btw I have my own toy C.Elegans simulator now: http://i.imgur.com/BlOJP9L.jpg 700 lines of CL code, 2 days of work. | 22:58 |
| kanzure | i think openworm is more accurate | 22:58 |
| kirka | No nervous system yet, just physics | 22:58 |
| kirka | kanzure Surely, you are right. I'm just playing | 22:59 |
| kanzure | okay | 22:59 |
| kanzure | have you ever used openworm? | 22:59 |
| kirka | I'm following that project, and I have read papers, but it's still on early stages: there are just some tech demos and incomplete neural network somulator | 23:00 |
| kirka | Openworm is a very interesting project | 23:02 |
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| kanzure | i think there's another simulator that you are skipping | 23:03 |
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| kirka | Nemaload? Or Cyber Elegans? | 23:03 |
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| kanzure | not sure, sorry | 23:03 |
| kirka | This inspired me https://www.youtube.com/watch?v=3uV3yTmUlgo | 23:04 |
| kirka | They have model of c.elegans neural network, but they cannot simulate it because of lack of synaptic weights | 23:06 |
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| kirka | kanzure Is there any list of DIYbio achievements? I mean successful transformation of organisms and other projects. | 23:14 |
| kanzure | there's http://diyhpl.us/wiki/diybio/faq/news | 23:16 |
| kanzure | but no there's only http://diyhpl.us/wiki/diybio/faq | 23:16 |
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| kirka | Looks interesting http://www.bio-rad.com/prd/en/US/LSE/PDP/619b8f74-9d3f-4c2f-a795-8a27e67598b7/pGLO-Bacterial-Transformation-Kit | 23:30 |
| kirka | But transformation isn't easy | 23:32 |
| kanzure | it's easy compared to other projects. | 23:32 |
| kirka | I should learn to culture ecoli first | 23:32 |
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| kirka | So, I'll have to build an incubator with thermostat, and I'll need something for heat-shocking ecoli | 23:36 |
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| kirka | Heat shock is done in water bath | 23:38 |
| kanzure | you could also use electroporation | 23:39 |
| yashgaroth | heat shock is easier | 23:40 |
| kirka | I understand. I'm thinking of simply DIUbio home lab setup. There is some information https://groups.google.com/forum/?fromgroups=#!topic/diybio/FlrxI3JcniE | 23:41 |
| kanzure | what is "DIU" | 23:41 |
| kanzure | oh a typo | 23:41 |
| kirka | DIY mistyped | 23:41 |
| kanzure | nevermind | 23:42 |
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| kirka | So, for that experiment http://www.bio-rad.com/webroot/web/images/lse/products/pglo_gfp_kits/product_overlay_content/global/pglo_bac_trans_kit_flowchart.jpg I'll need incubator and water bath. For doing simple experiments like this one making my own agar plates will be useful. Also I'll have to sterilize petri dishes before incubation. Is that enough for transformation? | 23:47 |
| yashgaroth | you don't necessarily need a waterbath, just a thermometer | 23:54 |
| yashgaroth | are you just gonna buy the kit and do that or something | 23:55 |
| kirka | Ok. | 23:56 |
| kirka | What was your first project in diybio? | 23:57 |
| yashgaroth | who, me? | 23:58 |
| kirka | you and kanzure | 23:58 |
| yashgaroth | I do bacterial transformations at work, I don't really bother replicating that at home | 23:59 |
| --- Log closed Tue May 21 00:00:29 2013 | ||
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