| --- Log opened Sat Jun 22 00:00:04 2013 | ||
| abetusk | So I hope I won't get lashed too harshly for such a general question, but how long, how feasible and what kind of technology needs to happen to get whole exome sequencing at the DIY or home biology level? | 00:13 |
|---|---|---|
| nmz787_ | you can probably do the extraction and prep in current DIY labs | 00:47 |
| -!- Juul [~Juul@50-0-83-158.dsl.static.sonic.net] has quit [Quit: mesh the planet!] | 01:28 | |
| -!- Viper168_ [~Viper@unaffiliated/viper168] has joined ##hplusroadmap | 02:38 | |
| -!- Viper168 [~Viper@unaffiliated/viper168] has quit [Ping timeout: 240 seconds] | 02:41 | |
| -!- Viper168_ is now known as Viper168 | 02:45 | |
| -!- Jaakko96 [~Jaakko@host86-153-84-34.range86-153.btcentralplus.com] has joined ##hplusroadmap | 02:52 | |
| -!- Jaakko96 [~Jaakko@host86-153-84-34.range86-153.btcentralplus.com] has quit [Client Quit] | 02:53 | |
| -!- Juul [~Juul@c-67-170-193-114.hsd1.ca.comcast.net] has joined ##hplusroadmap | 03:15 | |
| -!- lichen_ [~lichen@c-24-21-206-64.hsd1.or.comcast.net] has joined ##hplusroadmap | 03:42 | |
| -!- lichen [~lichen@c-24-21-206-64.hsd1.or.comcast.net] has quit [Ping timeout: 248 seconds] | 03:44 | |
| -!- EnLilaSko [EnLilaSko@unaffiliated/enlilasko] has joined ##hplusroadmap | 03:58 | |
| -!- makoLime [~mako@103-9-42-152.flip.co.nz] has quit [Ping timeout: 255 seconds] | 04:06 | |
| -!- chris_99 [~chris_99@unaffiliated/chris-99/x-3062929] has joined ##hplusroadmap | 04:22 | |
| -!- yorick [~yorick@oftn/member/yorick] has joined ##hplusroadmap | 04:38 | |
| -!- weles [~mariusz@c-71-234-3-169.hsd1.ct.comcast.net] has joined ##hplusroadmap | 04:43 | |
| -!- Juul [~Juul@c-67-170-193-114.hsd1.ca.comcast.net] has quit [Quit: Leaving] | 05:16 | |
| -!- ParahSai1in [~Rob@50-194-178-148-static.hfc.comcastbusiness.net] has joined ##hplusroadmap | 05:41 | |
| -!- ParahSailin [~Rob@50-194-178-148-static.hfc.comcastbusiness.net] has quit [Ping timeout: 256 seconds] | 05:44 | |
| -!- helleshin [~talinck@69-61-156-24.ubr1.dyn.lebanon-oh.fuse.net] has quit [Ping timeout: 276 seconds] | 06:05 | |
| -!- helleshin [~talinck@69-61-156-24.ubr1.dyn.lebanon-oh.fuse.net] has joined ##hplusroadmap | 06:25 | |
| -!- hehelleshin [~talinck@69-61-156-24.ubr1.dyn.lebanon-oh.fuse.net] has joined ##hplusroadmap | 06:29 | |
| -!- helleshin [~talinck@69-61-156-24.ubr1.dyn.lebanon-oh.fuse.net] has quit [Ping timeout: 246 seconds] | 06:32 | |
| -!- helleshin [~talinck@69-61-156-24.ubr1.dyn.lebanon-oh.fuse.net] has joined ##hplusroadmap | 06:32 | |
| -!- hehelleshin [~talinck@69-61-156-24.ubr1.dyn.lebanon-oh.fuse.net] has quit [Ping timeout: 268 seconds] | 06:35 | |
| -!- helleshin [~talinck@69-61-156-24.ubr1.dyn.lebanon-oh.fuse.net] has quit [Ping timeout: 256 seconds] | 06:38 | |
| -!- phillyj1 [~Thunderbi@pool-108-16-169-222.phlapa.east.verizon.net] has joined ##hplusroadmap | 06:48 | |
| -!- helleshin [~talinck@69-61-156-24.ubr1.dyn.lebanon-oh.fuse.net] has joined ##hplusroadmap | 06:49 | |
| -!- abetusk [~abetusk@cpe-24-58-232-122.twcny.res.rr.com] has quit [Ping timeout: 260 seconds] | 06:49 | |
| -!- hehelleshin [~talinck@69-61-156-24.ubr1.dyn.lebanon-oh.fuse.net] has joined ##hplusroadmap | 06:54 | |
| -!- helleshin [~talinck@69-61-156-24.ubr1.dyn.lebanon-oh.fuse.net] has quit [Ping timeout: 276 seconds] | 06:54 | |
| -!- phillyj1 [~Thunderbi@pool-108-16-169-222.phlapa.east.verizon.net] has quit [Quit: phillyj1] | 06:56 | |
| -!- hehelleshin [~talinck@69-61-156-24.ubr1.dyn.lebanon-oh.fuse.net] has quit [Ping timeout: 264 seconds] | 07:10 | |
| -!- helleshin [~talinck@69-61-156-24.ubr1.dyn.lebanon-oh.fuse.net] has joined ##hplusroadmap | 07:14 | |
| -!- chris_99 [~chris_99@unaffiliated/chris-99/x-3062929] has quit [Read error: No route to host] | 07:16 | |
| -!- chris_99 [~chris_99@unaffiliated/chris-99/x-3062929] has joined ##hplusroadmap | 07:17 | |
| -!- hehelleshin [~talinck@69-61-156-24.ubr1.dyn.lebanon-oh.fuse.net] has joined ##hplusroadmap | 07:33 | |
| -!- helleshin [~talinck@69-61-156-24.ubr1.dyn.lebanon-oh.fuse.net] has quit [Ping timeout: 255 seconds] | 07:34 | |
| -!- ThomasEgi [~thomas@panda3d/ThomasEgi] has joined ##hplusroadmap | 07:56 | |
| -!- yashgaroth [~ffffff@cpe-66-27-118-94.san.res.rr.com] has joined ##hplusroadmap | 08:41 | |
| -!- ianmathwiz7 [~chatzilla@ip65-46-170-6.z170-46-65.customer.algx.net] has joined ##hplusroadmap | 08:42 | |
| -!- phillyj [~Thunderbi@pool-108-16-169-222.phlapa.east.verizon.net] has joined ##hplusroadmap | 08:53 | |
| -!- phillyj [~Thunderbi@pool-108-16-169-222.phlapa.east.verizon.net] has quit [Client Quit] | 08:54 | |
| -!- ThomasEgi [~thomas@panda3d/ThomasEgi] has quit [Ping timeout: 255 seconds] | 08:57 | |
| -!- ThomasEgi [~thomas@panda3d/ThomasEgi] has joined ##hplusroadmap | 09:10 | |
| -!- abetusk [~abetusk@cpe-24-58-232-122.twcny.res.rr.com] has joined ##hplusroadmap | 09:20 | |
| -!- abetusk is now known as Guest81512 | 09:20 | |
| -!- weles [~mariusz@c-71-234-3-169.hsd1.ct.comcast.net] has quit [Ping timeout: 240 seconds] | 09:24 | |
| kanzure | Guest81512: you can do that at home now, but you would have to either refurbish a machine or build your own | 09:27 |
| -!- Guest81512 is now known as abetusk | 09:27 | |
| abetusk | kanzure, what now? | 09:27 |
| yashgaroth | whole exome sequencing | 09:29 |
| abetusk | ah. So whole exome sequencing is possible at the DIY level? | 09:30 |
| yashgaroth | with the sequencers available on ebay, it's possible but would take a really long time | 09:30 |
| abetusk | um, and maybe I should qualify that with cheap as well... | 09:30 |
| kanzure | refurbishing a sequencer is totes doable | 09:31 |
| kanzure | also, DIY does not necessarily mean cheap. get a job. | 09:31 |
| kanzure | *better job | 09:31 |
| yashgaroth | far cheaper to pay a company than to buy the sequencer/get it working, and all the reagents | 09:31 |
| abetusk | so the answer either way is throw more money at it | 09:32 |
| kanzure | well, you can build a sequencer if you want | 09:32 |
| yashgaroth | as with most of biology, yes | 09:32 |
| kanzure | i wish more people would choose the build option | 09:34 |
| kanzure | we would probably have actually good equipment by now if someone started working on a sequencer 5 years ago or w/e | 09:34 |
| yashgaroth | but but another pcr machine | 09:34 |
| kanzure | hah | 09:34 |
| kanzure | did you ever see jojack's pcr machine? | 09:34 |
| yashgaroth | was he on the lavaamp? | 09:35 |
| kanzure | yes | 09:35 |
| yashgaroth | never really looked into it because it's [another diy pcr machine] | 09:36 |
| kanzure | they spent $100k on it (or more). at least $100k went to rob carlson to make 1 prototype. | 09:36 |
| yashgaroth | hahaha | 09:37 |
| gradstudentbot | The paper was rejected. | 09:38 |
| yashgaroth | "The price point for new LavaAmps should be well underneath the several thousand dollars charged for educational thermocyclers that use heater blocks powered by peltier chips" oh it costs less than several thousand dollars? how did they possibly manage such a feat | 09:38 |
| abetusk | didn't you just show a video where jojack was complaining about inefficiencies? | 09:39 |
| kanzure | well..... yes. | 09:40 |
| yashgaroth | jojack complains about a lot of things, so probably | 09:40 |
| -!- weles [~mariusz@c-71-234-3-169.hsd1.ct.comcast.net] has joined ##hplusroadmap | 10:00 | |
| kanzure | front-end things :( https://gist.github.com/dypsilon/5819504 | 10:29 |
| kanzure | i guess i mean https://github.com/dypsilon/frontend-dev-bookmarks but this seems like a dumb way to store bookmarks (in a markdown file in git?) | 10:30 |
| kanzure | and his security stuff is weak. hah. | 10:30 |
| -!- Viper168 [~Viper@unaffiliated/viper168] has quit [Ping timeout: 276 seconds] | 10:42 | |
| -!- ianmathwiz7 [~chatzilla@ip65-46-170-6.z170-46-65.customer.algx.net] has quit [Ping timeout: 246 seconds] | 10:49 | |
| -!- ianmathwiz7 [~chatzilla@ip65-46-170-6.z170-46-65.customer.algx.net] has joined ##hplusroadmap | 10:49 | |
| -!- chris_99 [~chris_99@unaffiliated/chris-99/x-3062929] has quit [Remote host closed the connection] | 10:52 | |
| -!- chris_99 [~chris_99@unaffiliated/chris-99/x-3062929] has joined ##hplusroadmap | 10:53 | |
| heath | https://www.youtube.com/watch?feature=player_detailpage&v=AWEpeW7Ojzs#t=308s | 10:56 |
| heath | .title | 10:56 |
| yoleaux | Ellen Jorgensen: Biohacking -- you can do it, too - YouTube | 10:56 |
| heath | where is this United Nations study she mentions here? | 10:56 |
| kanzure | ugh there are makerbot stores in manhattan now | 11:32 |
| kanzure | heath: check the transcripts from fbi-diybio-2012. i think i asked that question and she responded. | 11:32 |
| heath | thanks reading now | 12:22 |
| heath | ok, i don't see anything to do with a report | 12:39 |
| heath | i'll email her | 12:39 |
| brownies | a makerbot *store*? as in, a store dedicated to selling makerbots? doesn't that seem a bit much? | 12:47 |
| kanzure | they probably sell plastic too | 12:48 |
| kanzure | but yes that does seem over-the-top | 12:48 |
| gradstudentbot | That's definitely a Nature paper. | 12:50 |
| kanzure | heath: unfortunately ellen does not believe in email (she only communicates with people she knows in person/sees daily) | 12:51 |
| -!- Burninate [~Burn@pool-108-18-159-219.washdc.fios.verizon.net] has quit [Read error: Connection reset by peer] | 12:58 | |
| -!- Burninate [~Burn@pool-108-18-159-219.washdc.fios.verizon.net] has joined ##hplusroadmap | 12:58 | |
| kanzure | webgl + articulated models http://alteredqualia.com/three/examples/webgl_animation_skinning_tf2.html | 13:23 |
| -!- phillyj [~Thunderbi@pool-108-16-169-222.phlapa.east.verizon.net] has joined ##hplusroadmap | 13:41 | |
| -!- Viper168 [~Viper@node60.19.251.72.1dial.com] has joined ##hplusroadmap | 14:00 | |
| -!- Viper168 [~Viper@node60.19.251.72.1dial.com] has quit [Changing host] | 14:00 | |
| -!- Viper168 [~Viper@unaffiliated/viper168] has joined ##hplusroadmap | 14:00 | |
| heath | maybe she was talking about fbi-diybio workshop | 14:03 |
| heath | and speaking with piers d millet | 14:04 |
| -!- phillyj [~Thunderbi@pool-108-16-169-222.phlapa.east.verizon.net] has quit [Quit: phillyj] | 14:04 | |
| heath | https://www.google.com/url?sa=t&rct=j&q=&esrc=s&source=web&cd=1&cad=rja&ved=0CC8QFjAA&url=http%3A%2F%2Funog.ch%2F80256EDD006B8954%2F(httpAssets)%2F2C8C2466169C45F5C1257770004E66B7%2F%24file%2FInternational%2Bperspectives%2B-%2BMillett.pdf&ei=cRHGUdjFHIjw8QSv1IGoDw&usg=AFQjCNFBFRDtE4JqF7K-b5fZK04Wlb7R9A&sig2=Ka_DgC8tHVJ-ioiPSaroEg | 14:06 |
| heath | .titlel | 14:06 |
| heath | .title | 14:06 |
| yoleaux | heath: Sorry, that doesn't appear to be an HTML page. | 14:06 |
| fenn | i thought you installed the extension to get rid of that link tracking crap | 14:08 |
| heath | me? | 14:10 |
| * heath isn't aware of an extension | 14:10 | |
| heath | i was just using scapy to find the exact URL... | 14:10 |
| fenn | oh nm that was nmz787 | 14:12 |
| kanzure | the actual url is in that url. just run it through urldecode | 14:12 |
| kanzure | +. | 14:12 |
| kanzure | or maybe it's uridecode | 14:13 |
| brownies | .meow | 14:17 |
| yoleaux | http://moar.edgecats.net/cats/XgSDwJH.gif | 14:17 |
| heath | http%3A%2F%2Funog.ch%2F80256EDD006B8954%2F(httpAssets)%2F2C8C2466169C45F5C1257770004E66B7%2F%24file%2FInternational%2Bperspectives%2B-%2BMillett.pdf | 14:17 |
| kanzure | now pass that through uridecode | 14:17 |
| heath | http://unog.ch/80256EDD006B8954/(httpAssets)/2C8C2466169C45F5C1257770004E66B7/$file/International+perspectives+-+Millett.pdf | 14:19 |
| heath | wheee | 14:19 |
| kanzure | oh i mean decodeURIComponent | 14:19 |
| heath | also on that site which is related... | 14:19 |
| heath | http://www.unog.ch/80256EE600585943/(httpPages)/98DD55F8A0EF259DC12574B200461162?OpenDocument | 14:19 |
| heath | .title | 14:19 |
| yoleaux | The United Nations in the Heart of Europe | Disarmament | BWC MX 2008 Synthetic Biology Seminar | 14:19 |
| kanzure | console.log(decodeURIComponent("http%3A%2F%2Funog.ch%2F80256EDD006B8954%2F(httpAssets)%2F2C8C2466169C45F5C1257770004E66B7%2F%24file%2FInternational%2Bperspectives%2B-%2BMillett.pdf")) | 14:19 |
| kanzure | http://unog.ch/80256EDD006B8954/(httpAssets)/2C8C2466169C45F5C1257770004E66B7/$file/International+perspectives+-+Millett.pdf | 14:19 |
| heath | https://www.youtube.com/watch?v=x5yPkxCLads | 14:22 |
| heath | .title | 14:22 |
| yoleaux | The PCR Song - YouTube | 14:22 |
| fenn | maybe yoleaux should just .title any bare youtube links on their own line | 14:24 |
| fenn | .decode "http%3A%2F%2Funog.ch%2F80256EDD006B8954%2F(httpAssets)%2F2C8C2466169C45F5C1257770004E66B7%2F%24file%2FInternational%2Bperspectives%2B-%2BMillett.pdf" | 14:26 |
| yoleaux | "http%3A%2F%2Funog.ch%2F80256EDD006B8954%2F(httpAssets)%2F2C8C2466169C45F5C1257770004E66B7%2F%24file%2FInternational%2Bperspectives%2B-%2BMillett.pdf" | 14:26 |
| fenn | .bruce | 14:35 |
| heath | .willis | 14:35 |
| fenn | http://tumbolia.appspot.com/fact/http://geekz.co.uk/schneierfacts/ | 14:37 |
| -!- makoLime [~mako@103-9-42-152.flip.co.nz] has joined ##hplusroadmap | 14:51 | |
| brownies | .meow | 15:08 |
| yoleaux | http://moar.edgecats.net/cats/tumblr_m3p7m9DutI1rows1to1_250.gif | 15:08 |
| -!- lichen_ [~lichen@c-24-21-206-64.hsd1.or.comcast.net] has quit [Quit: Lost terminal] | 15:37 | |
| -!- chris_99 [~chris_99@unaffiliated/chris-99/x-3062929] has quit [Quit: Leaving] | 16:03 | |
| -!- EnLilaSko [EnLilaSko@unaffiliated/enlilasko] has quit [Quit: - nbs-irc 2.39 - www.nbs-irc.net -] | 16:22 | |
| -!- lichen [~lichen@c-24-21-206-64.hsd1.or.comcast.net] has joined ##hplusroadmap | 17:01 | |
| -!- yorick [~yorick@oftn/member/yorick] has quit [Remote host closed the connection] | 17:30 | |
| -!- ThomasEgi [~thomas@panda3d/ThomasEgi] has quit [Remote host closed the connection] | 17:49 | |
| -!- lichen [~lichen@c-24-21-206-64.hsd1.or.comcast.net] has quit [Quit: Lost terminal] | 18:16 | |
| -!- Urchin[emacs] [~user@unaffiliated/urchin] has quit [Ping timeout: 268 seconds] | 18:47 | |
| -!- jonathan__ [~jonathan@wsip-70-167-73-242.sd.sd.cox.net] has joined ##hplusroadmap | 19:11 | |
| jonathan__ | paging max | 19:12 |
| jonathan__ | "Look at this amazing FOOD this 3D printer can make! NOTE: Food not edible." | 19:16 |
| yashgaroth | ha | 19:20 |
| yashgaroth | oh 3d printing, what won't you try to do badly and then hype the fuck out of it | 19:25 |
| -!- lichen [~lichen@c-24-21-206-64.hsd1.or.comcast.net] has joined ##hplusroadmap | 20:04 | |
| jonathan__ | the new black sabbath album is pretty sweet | 20:08 |
| heath | ellen got back with me | 20:34 |
| heath | http://igem.org/wiki/images/e/ec/UNICRI-synNanobio-final-2-public.pdf | 20:34 |
| heath | .title | 20:34 |
| heath | that's the link she gave | 20:34 |
| yoleaux | heath: Sorry, that doesn't appear to be an HTML page. | 20:34 |
| heath | """the part about fostering a culture of shared responsibility is in the summary on page 10 under "Response Options".""" | 20:38 |
| -!- hehelleshin [~talinck@69-61-156-24.ubr1.dyn.lebanon-oh.fuse.net] has quit [Ping timeout: 268 seconds] | 21:23 | |
| -!- helleshin [~talinck@69-61-156-24.ubr1.dyn.lebanon-oh.fuse.net] has joined ##hplusroadmap | 21:23 | |
| -!- klafka [~klafka@c-24-6-18-31.hsd1.ca.comcast.net] has quit [Remote host closed the connection] | 21:52 | |
| -!- Viper168_ [~Viper@node189.18.251.72.1dial.com] has joined ##hplusroadmap | 22:02 | |
| -!- Viper168_ [~Viper@node189.18.251.72.1dial.com] has quit [Changing host] | 22:02 | |
| -!- Viper168_ [~Viper@unaffiliated/viper168] has joined ##hplusroadmap | 22:02 | |
| -!- Viper168 [~Viper@unaffiliated/viper168] has quit [Ping timeout: 248 seconds] | 22:05 | |
| -!- superkuh [~superkuh@unaffiliated/superkuh] has quit [Quit: video games] | 22:19 | |
| -!- klafka [~klafka@c-24-6-18-31.hsd1.ca.comcast.net] has joined ##hplusroadmap | 23:03 | |
| -!- klafka [~klafka@c-24-6-18-31.hsd1.ca.comcast.net] has quit [Ping timeout: 264 seconds] | 23:08 | |
| -!- helleshin [~talinck@69-61-156-24.ubr1.dyn.lebanon-oh.fuse.net] has quit [Ping timeout: 276 seconds] | 23:36 | |
| -!- ianmathwiz7 [~chatzilla@ip65-46-170-6.z170-46-65.customer.algx.net] has quit [Ping timeout: 246 seconds] | 23:50 | |
| --- Log closed Sun Jun 23 00:00:04 2013 | ||
Generated by irclog2html.py 2.15.0.dev0 by Marius Gedminas - find it at mg.pov.lt!
