| --- Log opened Sat Aug 03 00:00:44 2013 | ||
| heath | .title https://www.youtube.com/watch?v=xs1u3dIRcyY | 00:14 |
|---|---|---|
| yoleaux | Dr. Laura Landweber - "Epigenetics: Rewriting the information in DNA" - YouTube | 00:14 |
| heath | fenn: that would be the better video | 00:14 |
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| strangewarp | http://www.dhs.gov/how-we-fuck-you-in-your-ass - hmm | 02:05 |
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| -!- Topic for ##hplusroadmap: biohacking, nootropics, transhumanism, open hardware | sponsored by george church and the NRA | http://gnusha.org/logs http://diyhpl.us/wiki http://groups.google.com/group/diybio | banned by the Federal Death Administration | official paperbot fan club | 06:54 | |
| -!- Topic set by kanzure [~kanzure@131.252.130.248] [Sat Mar 23 20:40:45 2013] | 06:54 | |
| [Users ##hplusroadmap] | 06:54 | |
| [@bkero ] [ chris_99 ] [ heath ] [ nully ] [ Shehrazad_ ] [ underscor ] | 06:54 | |
| [@kanzure ] [ cogitokat ] [ HEx1 ] [ oblique ] [ sivoais ] [ upgrayeddd] | 06:54 | |
| [@nmz787 ] [ Coornail ] [ ivan` ] [ paperbot ] [ smeaaagle ] [ Urchin ] | 06:54 | |
| [ abetusk ] [ devrandom ] [ jrayhawk_ ] [ ParahSailin ] [ spresser ] [ yoleaux ] | 06:54 | |
| [ AlonzoTG ] [ EnLilaSko ] [ juri_ ] [ ParaSa1lin ] [ strangewarp] [ yorick ] | 06:54 | |
| [ archels ] [ fenn ] [ juul ] [ pasky ] [ streety ] [ Zhwazi ] | 06:54 | |
| [ AshleyWaffle] [ fredox ] [ lichen ] [ phryk ] [ superkuh ] [ zubaz ] | 06:54 | |
| [ audy ] [ gnusha ] [ lupfantomo ] [ randallagordon] [ Thomas42_ ] | 06:54 | |
| [ balrog ] [ gnusha_ ] [ monkeynipples] [ rigel ] [ ThomasEgi ] | 06:54 | |
| [ brownies ] [ gradstudentbot] [ nsh ] [ ryankarason ] [ Thorbinator] | 06:54 | |
| [ Burninate ] [ Guest10542 ] [ nuba_ ] [ saurik ] [ ua ] | 06:54 | |
| -!- Irssi: ##hplusroadmap: Total of 62 nicks [3 ops, 0 halfops, 0 voices, 59 normal] | 06:54 | |
| -!- Channel ##hplusroadmap created Thu Feb 25 23:40:30 2010 | 06:54 | |
| -!- Irssi: Join to ##hplusroadmap was synced in 8 secs | 06:54 | |
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| @kanzure | bleep boop | 07:49 |
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| fredox | that reminds me | 07:54 |
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| @kanzure | fredox: ? | 08:25 |
| @kanzure | yashgaroth: sup | 08:25 |
| yashgaroth | apparently the guy who brought in a bunch of equipment to the lab has been asked to remove it by assaydepot guy | 08:26 |
| fredox | i never grabbed the last venture bros | 08:26 |
| ParaSa1lin | brock dies | 08:27 |
| @kanzure | brock dies? aw fuck. | 08:29 |
| @kanzure | oh that's right, the later seasons have some other voice actor | 08:29 |
| @kanzure | yashgaroth: so they just didn't want the equipment? | 08:39 |
| yashgaroth | I doubt it, since it was over half of our stuff...maybe some sort of personal falling-out that everyone else has to deal with | 08:39 |
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| yashgaroth | admittedly 90% of his stuff was obsolete and/or broken but still, the one working -80 and floor centrifuge and power supplies and such | 08:40 |
| ParaSa1lin | how was nmz787's spec going anyway | 08:40 |
| gradstudentbot | Where are the hot plates? | 08:40 |
| ParaSa1lin | thats a project with some prospect | 08:40 |
| @kanzure | ParahSailin: i think he's still busy trying to find someone who knows optics things | 08:41 |
| ParaSa1lin | put out a craigslist ad for one | 08:51 |
| ParaSa1lin | or buy one of the chinese knockoffs and reverse engineer a reverse engineered nanodrop | 08:51 |
| ParaSa1lin | id be willing to pay a little extra to see it complete sooner | 08:54 |
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| @kanzure | yeah i don't remember what his holdup is. i thought it was optics, like sourcing a lense or something? | 09:13 |
| @kanzure | http://tequals0.wordpress.com/2013/08/03/the-gude-to-diy-gel-illumination/ | 09:14 |
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| @kanzure | oh.. hello me, https://gist.github.com/kanzure/5558267 | 09:45 |
| @kanzure | i wish i would have also published commit-cleaner.py because it's currently on another machine | 09:45 |
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| abetusk | Did you see the guideline change on Kickstarter? "Projects cannot offer genetically modified organisms as a reward." http://www.kickstarter.com/help/guidelines | 10:41 |
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| @kanzure | yep | 10:50 |
| abetusk | I think it has a chilling effect on future projects. Very short sighted | 11:06 |
| @kanzure | it doesn't matter, that's just kickstarter | 11:08 |
| abetusk | it does matter. Biology projects will benefit from crowd funding, of which Kickstarter is a major player | 11:13 |
| @kanzure | kickstarter isn't doing the crowdfunding | 11:14 |
| @kanzure | i think you have it backwards? | 11:14 |
| cogitokat | How do I respond to a message that was sent before I joined the mailing list? | 11:15 |
| abetusk | they facilitate crowd funding. They provide the infrastructure for crowd funding projects | 11:15 |
| @kanzure | abetusk: i think you can just scrape the list of people who donated to biocurious/glowing plant/whatever and just email them directly. problem solved. | 11:16 |
| abetusk | kanzure, I'm not so sure. That list is certainly important, but I think there is a large market for people with low involvement in the DIY bio community that would like to donate/fund/invest in such projects | 11:17 |
| @kanzure | it's not investing | 11:17 |
| yashgaroth | kickstarter projects have all the donors' emails listed publicly? | 11:17 |
| @kanzure | no, but i have their emails because i stalked everyone | 11:17 |
| yashgaroth | haha fair enough | 11:17 |
| @kanzure | and i have a database of people who have monetarily contributed to those projects | 11:17 |
| @kanzure | (because fuck kickstarter) | 11:17 |
| abetusk | it depends on how liberal your definition of investing is. | 11:17 |
| abetusk | When I fund open source projects, I consider it an investment | 11:18 |
| @kanzure | maybe you should stick with SEC definitions | 11:18 |
| abetusk | fair enough | 11:18 |
| @kanzure | heh | 11:18 |
| @kanzure | anyway, i think if those people exist then they would also exist outside of kickstarter.com | 11:18 |
| abetusk | And you could certainly go to some other crowd source funding sight, create your own, etc., but Kickstarter is one of the largest | 11:19 |
| abetusk | *site | 11:19 |
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| @nmz787 | ParaSa1lin: being lazy on layout... circuit schematic is done, started layout.. need to get through it once and have a mixed-signal guru look it over | 12:12 |
| @nmz787 | optics are simple, if the chinese company didn't rip me off for a slug of random plastic with lines on it, it should be plug n chug (or play) | 12:12 |
| @nmz787 | (simple was a huge realization, after I learned how complex it all really is) | 12:13 |
| @nmz787 | simple but it has to be done right | 12:13 |
| @nmz787 | figuring out what is right was hard | 12:13 |
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| fredox | "Taq works best at a G/C content of no more than 50%. Addition of extra Mg2+ and optimization will usually work for up to 60%. For even higher G/C templates, Taq is unreliable." -> OpenTaq = 59% gc | 12:41 |
| @kanzure | what is opentaq? | 12:43 |
| @kanzure | do you mean one of the unpatented taq variants? | 12:43 |
| yashgaroth | do you mean the gene sequence for the opentaq protein is 59% gc? because that's irrelevant to what sequences the protein will amplify | 12:47 |
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| fredox | sequence is open source -> protein is sold. Idea was use sequence as test run for synth, then use to make your own protein. kinda ironic that it dosen't like amping its own source | 12:54 |
| fredox | kanzure: http://www.openbiotech.com/v/vspfiles/assets/images/popentaq%20datasheet.pdf | 12:55 |
| @kanzure | i'm confused about what you are complaining about | 13:03 |
| @kanzure | can you state your complaint in the form of heckling? | 13:03 |
| @kanzure | also, i forgot that john called his thing opentaq. that's sort of a douche move but whatever. from henceforth we shall refer to that as schloendorn taq. | 13:04 |
| fredox | Hey Taq, why dont you go amplify yourself? Oh thats right you cant hahaha | 13:04 |
| @kanzure | unreliable doesn't mean it never works | 13:04 |
| fredox | yeah but i dont want use something that apparently unrelaible as a test for prototype function verifaction | 13:06 |
| fredox | fication even | 13:06 |
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| @kanzure | eh, you could just sample a few thousand different polymerases i guess | 13:21 |
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| @kanzure | try searching through ncbi for polymerases that have an even distribution of nucleotides | 13:22 |
| fredox | I've just beeen looking around for a good simple target to test in the kb range. seemed fortuitous to notice that | 13:23 |
| fredox | but yeah i'll have a look | 13:24 |
| @kanzure | most people use gfp because they can force an ecoli to express it | 13:24 |
| yashgaroth | what about, like, the rest of the opentaq plasmid? looks like it's about 50/50 gc | 13:25 |
| @kanzure | most oligo synthesizers can't synthesize gfp | 13:25 |
| @kanzure | (without forcing you to manually ligate oligos together) | 13:29 |
| @kanzure | but if you're okay with ligation then you should be okay with synthesizing 25 bp fragments at a time | 13:29 |
| @kanzure | so.. | 13:29 |
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| anannie | Okay, so for week 1 I created a three wheeled drag racer... (I didn't have a differential ) | 16:09 |
| anannie | http://imgur.com/a/IjHXm | 16:09 |
| @kanzure | where are the leocad files | 16:10 |
| anannie | kanzure: Leocad doesn't run on my tiny netbook I'm afraid | 16:11 |
| @kanzure | because of ram? | 16:11 |
| anannie | Yes and the lousy atom CPU | 16:11 |
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| @kanzure | not because of architecture? | 16:12 |
| anannie | ? | 16:13 |
| @kanzure | what? | 16:13 |
| @kanzure | do you get an error message when you try to run leocad? | 16:13 |
| anannie | It's been a while | 16:15 |
| anannie | I don't remember | 16:15 |
| anannie | I'll try to do it again and I'll tell you what happens | 16:17 |
| ThomasEgi | anannie, that thing needs propper bearings and about 300W more power output on the wheels ;) | 16:17 |
| anannie | http://imgur.com/a/cHAN7#0 <--- corrected the photos | 16:18 |
| anannie | ThomasEgi: Heh. I'm afraid that's the best I can do right now | 16:18 |
| ThomasEgi | you'll grow up to play with bigger motors one day | 16:20 |
| anannie | ThomasEgi: I already have... | 16:23 |
| ThomasEgi | grown up or played with bigger motors ;) | 16:24 |
| anannie | I've played with bigger motors | 16:25 |
| anannie | and I'm still growing up, so there isn't anything I can say over there... | 16:26 |
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| ThomasEgi | growing up ain't that important. the motors however.. are so fun :3 | 16:38 |
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| @nmz787 | looks cool anannie | 17:51 |
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| AshleyWaffle | relevant cause diy: http://www.youtube.com/watch?v=W9cK_TMLvjI | 19:36 |
| @kanzure | .title | 19:38 |
| yoleaux | Soda can Stirling engine - 860 rpm - YouTube | 19:38 |
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| anannie | I've been reading the wikipedia article for stirling engines AshleyWaffle and even though I studied thermodynamics intently once upon a time, it isn't immediately obvious to me why this works | 20:37 |
| AshleyWaffle | heh | 20:37 |
| anannie | I mean I've read about the entire carnot cycle, but I don't grok why on earth this actually works | 20:40 |
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| tomkinsc | paperbot: http://link.springer.com/article/10.1007/BF00028018#page-1 | 22:21 |
| paperbot | http://libgen.org/scimag2/10.1007/BF00028018.pdf | 22:21 |
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| anannie | brownies: I didn't fancy seeing you in here :) | 23:30 |
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| --- Log closed Sun Aug 04 00:00:03 2013 | ||
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