| --- Log opened Thu Nov 21 00:00:31 2013 | ||
| Qfwfq|work | paperbot: http://dx.doi.org/10.1016%2F0012-365X%2894%2990019-1 | 00:08 |
|---|---|---|
| paperbot | http://diyhpl.us/~bryan/papers2/paperbot/afbf5d08fbab9d23e41ac1ecdcb3c61a.txt | 00:08 |
| nmz787 | next-gen breast implants: http://ww2.biol.sc.edu/~bergerlab/599%20PAPERS/pdfs/shackleton%202006.pdf | 00:37 |
| nmz787 | Generation of a functional mammary gland from a | 00:38 |
| nmz787 | single stem cell | 00:38 |
| nmz787 | how the hell would you even think of going about engineering a cow to produce the milk of a seal | 00:39 |
| nmz787 | get transcriptomes for a bunch of low-fat and high-fat populations of cow and seal.... find which genes were varying, then swap the seal set into the cow... but that wouldn't impact development | 00:41 |
| nmz787 | unless you time-sampled the mRNA too maybe | 00:41 |
| nmz787 | you might need an end-point measurement to be based on something that would corellate to a difference in anatomy, maybe viscosity? | 00:43 |
| nmz787 | "Moose milk has the consistency of thick cream and has the odor of fresh bovine milk" (10% fat) | 00:45 |
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| kanzure | "Mikhail Kazakov from OpenCascade SAS just announced at the Salome User's Day that next OCCT version will be LGPL" | 08:23 |
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| juri_ | Interesting. | 09:19 |
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| EnLilaSko | paperbot: http://www.sciencedirect.com/science/article/pii/S0304394013004370 | 12:20 |
| paperbot | http://diyhpl.us/~bryan/papers2/paperbot/3ef9e4f0d2e739d69575ae60748d3b57.txt | 12:20 |
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| delinquentme | paperbot, http://adsabs.harvard.edu/abs/2011TRACE..26..371S | 13:05 |
| paperbot | http://diyhpl.us/~bryan/papers2/paperbot/ab06cfd9608f1aaa768ff8a9c583b0ff.txt | 13:06 |
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| superkuh | paperbot: http://www.cell.com/fulltext/S0092-8674(13)01360-3 | 13:44 |
| paperbot | http://diyhpl.us/~bryan/papers2/paperbot/eaa8588c6fd0cf54dc0a4bc3a85ce4d1.txt | 13:44 |
| superkuh | paperbot: http://www.sciencedirect.com/science?_ob=GatewayURL&_origin=CELLPRESS&_urlversion=4&_method=citationSearch&_version=1&_piikey=S0092867413013603&md5=1372a5392a55ba9fcde0013ce8caced6 | 13:45 |
| paperbot | http://diyhpl.us/~bryan/papers2/paperbot/1e48aabe5b37c65037380b72f45bd07f.txt | 13:45 |
| superkuh | paperbot: http://www.sciencedirect.com/science/article/pii/S0092867413013603/pdfft?md5=5727a579ed38b46c183779f13c7afeda&pid=1-s2.0-S0092867413013603-main.pdf | 13:46 |
| paperbot | http://diyhpl.us/~bryan/papers2/paperbot/1eb9a1ba1b00faa5b134c25ecf0c527d.pdf | 13:46 |
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| kanzure | pdfcoin | 13:47 |
| eudoxia | you mine it by freeing papers from paywalls? | 13:48 |
| kanzure | i dunno. probably something about doi numbers. | 13:49 |
| nmz787 | that would be cool | 13:54 |
| nmz787 | superkuh: still need those papers? | 13:56 |
| superkuh | I got them. | 13:58 |
| superkuh | Through paperbot. All requests were 1 paper. | 13:58 |
| superkuh | Just had to dig for the correct URL to proceed to the next stage a couple times. | 13:59 |
| kanzure | eudoxia: i don't think it would work | 13:59 |
| nmz787 | superkuh: so I've heard of COX-2 before, but can't place what it's importance is... they sell inhibitors as common drugs | 14:03 |
| superkuh | Ibuprofen. | 14:04 |
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| nmz787 | hmm, which I don't take often at all, maybe once every year or two | 14:05 |
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| superkuh | The OTC COX inhibitors are actually non-specific to COX-1 and COX-2. The former being important for the mucosal lining of the stomach. Thus the suggest to take them with food. | 14:06 |
| superkuh | In this paper they seem to use one of the later generation COX-2 specifics. | 14:07 |
| superkuh | (wherein the first gens had bad heart side effects) | 14:07 |
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| nmz787 | the wiki article on retinal detachment said THC could exacerbate... which seems contrary to all the non-scientific buzz about it being good for glaucoma (which I haven't actually found solid evidence supporting that it actually does help the condition) | 14:11 |
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| superkuh | Yes. The eye specific interactions have worried me. | 14:18 |
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| nmz787 | http://transova.com/our-services/genetic-preservation-cloning.php | 15:19 |
| nmz787 | http://www.viagen.com/our-services/cloning.php | 15:19 |
| nmz787 | "To clone an animal, ViaGen needs a sample of the animals genes, in the form of an ear punch or small biopsy sample. ViaGen provides a biopsy kit and a shipping container for transport of the biopsy sample to our lab." | 15:20 |
| nmz787 | "At our lab we culture the animals cells, then transfer DNA from those cells into eggs from which we have removed the genetic material. We grow the resulting embryos in an incubator for several days, then transfer them to our recipient females using a traditional embryo transfer process. After a normal gestation period, the cloned calves, piglets, or foals are born." | 15:20 |
| nmz787 | "Calves are picked up at approximately seven months of age, and foals at 60 days of age. With pigs, pregnant gilts are picked up at 60 days of pregnancy. All cloned animals are subjected to DNA testing to confirm their identities, and to veterinary examinations to confirm their health status." | 15:20 |
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| phillyj | oo, lots of ppl | 15:33 |
| phillyj | hi all | 15:33 |
| kanzure | phillyj: hi | 15:34 |
| nmz787 | hi | 15:34 |
| nmz787 | long-time! | 15:34 |
| phillyj | yea | 15:34 |
| phillyj | nmz787: you're on the west coast now? | 15:35 |
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| nmz787 | yeah | 15:39 |
| nmz787 | Oregon is great, naturally lush, so great bio-wise | 15:39 |
| nmz787 | no biotech or bioinformatics work though | 15:40 |
| nmz787 | some cancer and medical device companies, but nothing interesting anyway | 15:40 |
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| nmz787 | phillyj: what have you been up to? | 15:45 |
| phillyj | usual, working at the same place | 15:45 |
| phillyj | get a trickle of projects, cloning and such | 15:46 |
| phillyj | one of the bigger biotechs in my building went under | 15:47 |
| phillyj | they're selliing off their equipment | 15:48 |
| phillyj | not sure if the boss will get anything | 15:48 |
| phillyj | hope there's some cheap stuff | 15:48 |
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| kanzure | phillyj: what equipment is available? do you have storage? | 15:51 |
| phillyj | lots of stuff, centrifuges, freezers | 15:52 |
| phillyj | i have to get the list | 15:52 |
| phillyj | storage space meaning? | 15:52 |
| kanzure | if you can get a picture of the list or type up the actual list, then i'd like to see it | 15:52 |
| kanzure | meaning if i buy some of it, can you store it or arrange for it to be moved off of their location | 15:52 |
| phillyj | i'll have to ask them about it | 15:53 |
| phillyj | but i guess they'd be happy if you buy stuff | 15:53 |
| phillyj | i'm guessing its pretty new equipment because they moved in early this year | 15:54 |
| phillyj | kanzure: there is some space in my lab, it's not too big though and I am not sure if the landlord will let me leave stuff outside the lab for too long | 15:56 |
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| phillyj | kanzure: how interested are you? | 15:57 |
| kanzure | 5-10% | 15:57 |
| phillyj | depending on the equipment? Im in PA btw | 15:58 |
| nmz787 | heading to pittsburgh in the next 2 weeks phillyj ? | 16:00 |
| phillyj | nmz787: what? | 16:00 |
| nmz787 | are youheading to pittsburgh in the next 2 weeks? | 16:00 |
| phillyj | no | 16:00 |
| phillyj | why> | 16:00 |
| phillyj | ? | 16:01 |
| nmz787 | I am :P | 16:01 |
| nmz787 | too far apart too just come hang out in philly | 16:01 |
| nmz787 | or vice versa | 16:01 |
| nmz787 | unless you had plans already | 16:01 |
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| nmz787 | phillyj: do you just do single-cell organism cloning? | 16:04 |
| nmz787 | err, you mean cloning cloning, not SCNT | 16:05 |
| nmz787 | right? | 16:05 |
| phillyj | o | 16:05 |
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| phillyj | cloning cloning | 16:05 |
| phillyj | lol | 16:05 |
| phillyj | sry | 16:06 |
| nmz787 | I was just looking at this http://www.viagen.com/our-services/cloning.php | 16:06 |
| phillyj | yea, we do most basic molecular bio stuff | 16:07 |
| phillyj | lots of plasmid construction, mutagenesis | 16:07 |
| phillyj | or qpcr work if it comes our way | 16:07 |
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| phillyj | kanzure: sent you the equipment list | 16:15 |
| juri_ | i don't suppose anyone here is set up to produce DLC via the hot wire method here? | 16:17 |
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| kanzure | phillyj: neato, thanks | 16:18 |
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| phillyj | kanzure: did you see anything interesting in the list? | 16:36 |
| kanzure | diode array or refractometer might be interesting | 16:37 |
| phillyj | they're not big equipment, right? | 16:37 |
| kanzure | i don't think there's anything on this list i really really want | 16:38 |
| kanzure | is "TMTC" an abbreviation of "too many to count"? | 16:38 |
| kanzure | "Freezer -40C TMTC" ? | 16:38 |
| phillyj | thats what i think | 16:38 |
| phillyj | no | 16:39 |
| phillyj | theres 1 -40 | 16:39 |
| phillyj | u went cross-eyed | 16:39 |
| kanzure | damn | 16:40 |
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| phillyj | i might get the gloves, need to order more anyway | 16:45 |
| kanzure | that sounds exciting | 16:47 |
| phillyj | lol | 16:47 |
| phillyj | money is tight | 16:47 |
| phillyj | can only get essentials | 16:47 |
| kanzure | nah it's cool, protection for employees is BS anyway | 16:47 |
| phillyj | lollol | 16:47 |
| kanzure | and protection for their projects | 16:47 |
| kanzure | "more RNAses from skin contact? sure why not" | 16:48 |
| phillyj | that company was a big deal, but I heard they had "money mismanagement" at the highest level | 16:49 |
| phillyj | one monday, the parking lot it empty; they just packed up over the weekend | 16:49 |
| kanzure | definitely the preferred method of biological waste disposal | 16:50 |
| phillyj | 1 to 5 mil in revenue with up to 50 employees | 16:51 |
| phillyj | the lab folks all probably got laid off | 16:51 |
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| kanzure | "Almost sounds like it would be worth having some sort of software keep their eyes open for CryptoLocker and grab the AES key, if possible, proactively." | 19:39 |
| kanzure | (or just detect cryptolocker in the first place) | 19:40 |
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| kanzure | silk road timeline http://antilop.cc/sr/ | 20:05 |
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| kanzure | libgen asking for funding: http://genofond.org/viewtopic.php?f=8&t=7061 | 22:00 |
| kanzure | "The six months have expired and the time has come again to renew the seedbox lease to keep on distributing Library Genesis via torrents. The parameters are the same as before: 3TB x 4 , speed 100-800 Mbps. | 22:01 |
| kanzure | 1) the rent cost of the current configuration for six months amounts to $ 950. | 22:01 |
| kanzure | 2) the disc space ended at torrent 980 000 and a hardware upgrade is currently contemplated, configurations and prices are being negotiated. | 22:01 |
| kanzure | 3) the primary server of Library Genesis is to be upgraded as well. We are considering to take OCZ RevoDrive 3 X2 240G (for database) + several HDD to 4 TB for approximately $ 1000. | 22:01 |
| kanzure | geeze this is actually cheap | 22:01 |
| kanzure | "We accept: PayPal, YandexMoney, Webmoney, Bitcoin (link on the main page)" | 22:01 |
| kanzure | bitcoin donations have amounted to only 0.0222 wtf | 22:02 |
| kanzure | their bitcoin address is 1ENFY4h7ntGZbqwcwpQtXVFJrPnfXRHQLe | 22:05 |
| kanzure | http://blockchain.info/address/1ENFY4h7ntGZbqwcwpQtXVFJrPnfXRHQLe "total received: 0.98889322 final balance: 0.48879322" | 22:07 |
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| --- Log closed Fri Nov 22 00:00:32 2013 | ||
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