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| jrayhawk | I think I still have some deployed servers with reiserfs. | 02:12 |
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| kanzure | jrayhawk: the not knowing seems like the best part, there | 07:50 |
| eudoxia | somebody should fork the filesystem and give it another name | 07:51 |
| eudoxia | you can't have a conversation about reiserfs without someone bringing up the whole wifey knifey thing | 07:51 |
| kanzure | actually it was the other way around | 07:52 |
| kanzure | you can'thavea conversation about the princess bride without it turning into either (a) an argument about the dread pirate roberts or (b) an argument about filesystems | 07:52 |
| kanzure | *you can't have a | 07:54 |
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| entelechios | paperbot: http://prl.aps.org/abstract/PRL/v98/i5/e053902 | 11:41 |
| paperbot | http://diyhpl.us/~bryan/papers2/paperbot/a3649d73c5531070104821435811e1c3.txt | 11:41 |
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| jrayhawk | It's pretty good as a boot partition since it's basically indestructable, but I never really pay attention to boot partitions after I make them. | 11:50 |
| jrayhawk | Responds more poorly to memory corruption than most, though. | 11:51 |
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| delinquentme | kanzure:say I've got a number of illustrator path files for microfluidics | 12:10 |
| delinquentme | editing them in linux | 12:11 |
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| jrayhawk | Also has unusually fast tail-packing support, so was good for mail systems. | 12:33 |
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| kanzure | these are cool: http://tryerlang.org/ http://tryhaskell.org/ http://tryruby.org/ http://try-python.appspot.com/ http://repl.it/ | 15:10 |
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| heath | yeah | 15:18 |
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| entelechios | kanzure http://runnable.com/ | 15:21 |
| entelechios | it's not hugely hugely replete yet, but the notion-searching feature i find is pretty cool | 15:21 |
| kanzure | where's my launch on heroku button | 15:23 |
| kanzure | needs more deploy buttons | 15:24 |
| venturecommunist | hi kanzure | 15:27 |
| delinquentme | rofl | 15:27 |
| delinquentme | ^^ | 15:27 |
| delinquentme | dat handle | 15:27 |
| venturecommunist | thanks | 15:28 |
| venturecommunist | kanzure: i'm trying to evaluate a biohacking experiment for cost, what equipment would be needed, think i could pick your brain a bit? | 15:29 |
| venturecommunist | basically it's DRACO on rhinovirus | 15:29 |
| ParahSailin | venturecommunist: DRACO? | 15:29 |
| venturecommunist | https://en.wikipedia.org/wiki/DRACO_%28antiviral%29 | 15:29 |
| ParahSailin | what exactly you want to do | 15:31 |
| venturecommunist | so i guess send away to lifetechnologies.com for HIV1 TAT + PKR + ADAF1 | 15:31 |
| venturecommunist | heat shock transform into E. coli if it doesn't come in E. coli already | 15:31 |
| venturecommunist | then liquid culture in some kind of diy bioreactor | 15:32 |
| ParahSailin | you want to have someone synthesize genes? | 15:32 |
| ParahSailin | that would be idt, not lifetechnologies | 15:32 |
| venturecommunist | still looking on openwetware.org to see if i can do it with some open PCR variant | 15:32 |
| ParahSailin | shits unreliable enough as it is without crippling yourself with inferior equipment | 15:33 |
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| ParahSailin | what would pkr and adaf1 do in e coli? | 15:36 |
| venturecommunist | the genes would produce the proteins, if they're expressed in e coli | 15:37 |
| ParahSailin | ok.. | 15:37 |
| venturecommunist | the protein being a chimera of TAT, PKR and ADAF1 | 15:37 |
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| ParahSailin | what do you want that for? | 15:38 |
| ParahSailin | i dont even see an adaf1 in genbank | 15:38 |
| venturecommunist | if it's really non-toxic in-vivo in humans presumably it's a cure for AIDs, HPV, HSV, Hep A/B/C/D/E Ebola, Influenza and the Common Cold | 15:39 |
| ParahSailin | the draco antiviral drugs are the simple fusion protein you described? | 15:40 |
| venturecommunist | right | 15:40 |
| ParahSailin | hm good catch on the paper | 15:42 |
| venturecommunist | interesting right | 15:43 |
| ParahSailin | thats apaf1 btw :) | 15:43 |
| venturecommunist | apaf1 indeed | 15:44 |
| venturecommunist | apoptosis factor 1 or something | 15:44 |
| ParahSailin | PKR is the dsRNA detector? | 15:45 |
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| venturecommunist | right | 15:45 |
| venturecommunist | PKR and APAF1 are both human genes, TAT is an HIV1 gene | 15:45 |
| venturecommunist | HIV appears to have two basic methods of counter-acting PKR (which complexly will induce apoptosis) | 15:46 |
| venturecommunist | one is that TAT binds with PKR obstructing its action | 15:46 |
| venturecommunist | the other has to do with phosphorylation of something acted on by PKR | 15:47 |
| venturecommunist | but with a TAT already on there, it should be able to get anywhere HIV can get, without obstruction by HIV one would think | 15:47 |
| venturecommunist | and the APAF1s should be triggered independent of this phosphorylation issue cause it's a more direct pathway | 15:48 |
| ParahSailin | what you gonna test the drug on? | 15:48 |
| venturecommunist | there's definitly indication of PKR activation in HIV infected clls | 15:48 |
| venturecommunist | myself of course | 15:48 |
| venturecommunist | for make glorious venture communism | 15:48 |
| ParahSailin | might try an animal first | 15:49 |
| venturecommunist | i'm a human-imal | 15:49 |
| gradstudentbot | I don't think our fume hood is safe. | 15:50 |
| kanzure | get back in there | 15:51 |
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| venturecommunist | kanzure: lol | 15:53 |
| venturecommunist | i was just talking about the price of a fume hood today | 15:53 |
| venturecommunist | $80k for real? | 15:53 |
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| ParahSailin | im sure there exist ones that expensive | 16:03 |
| kanzure | "premium fume hoods" | 16:05 |
| kanzure | on etsy | 16:06 |
| kanzure | oh i'm sorry, tindie, right | 16:07 |
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| brownies | jrayhawk: you around? | 22:22 |
| gradstudentbot | I don't remember the paper, but someone definitely did that. | 22:23 |
| justanotheruser | gradstudentbot: is there a free algorithm for verifiable computation | 22:25 |
| gradstudentbot | Nope, that's not even remotely possible. | 22:25 |
| justanotheruser | oh :( | 22:25 |
| juri_ | gradstudentbot: are you watson, gathering information for the singularity, making sure humanity doesn't surpass or merge with the machines? | 22:30 |
| gradstudentbot | Should I be reading this? | 22:30 |
| juri_ | I knew it. | 22:30 |
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| jrayhawk | brownies: mostly | 22:37 |
| brownies | jrayhawk: educate me about pre- and post-workout nutrition? | 22:38 |
| brownies | or throw some papers at me, or something? | 22:38 |
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| jrayhawk | Depends on the modality, but, during training, typically folks aim for forcing fatty acid metabolic adaptation through carb-fasting pre-workout and then aim for a big carb and protein load post-workout while glycogen depleted in order to use an insulin spike to upregulate the thyroid for a week. | 22:42 |
| jrayhawk | Then abuse carbs during competition as needed. | 22:42 |
| brownies | so... protein shake before, then protein shake and food after? | 22:43 |
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| jrayhawk | I usually aim for fast-absorbing stuff to drive the spike as high as practical; pre-emulsified BCAAs in the form of egg and a couple tablespoons of some form of straight glucose, such as coconut sugar or corn dextrose or something. | 22:45 |
| brownies | i see | 22:46 |
| jrayhawk | If you don't particularly care about the metabolic floor provided by fatty acid metabolism, then you can be a lot looser on pre-workout nutrition. | 22:46 |
| brownies | i don't suppose you have any particular product recommendations to make this idiot-proof for me? | 22:47 |
| brownies | i read this http://www.consumerreports.org/cro/magazine-archive/2010/july/food/protein-drinks/what-our-tests-found/index.htm | 22:47 |
| brownies | and i've deduced that i should stop drinking muscle milk | 22:47 |
| jrayhawk | http://www.omgwallhack.org/home/jrayhawk/img/self/poiseanddignity.jpg fwiw here's observational evidence that i am at least not screwing up too badly | 22:48 |
| venturecommunist | well as arnold used to say when they asked if he won mr. universe by drinking milk | 22:49 |
| brownies | this photo appears to be upside-down | 22:49 |
| venturecommunist | "milk is foh baybeez" | 22:49 |
| brownies | oh, wait, i see. | 22:49 |
| brownies | yes, you seem to have some idea about what you're doing. | 22:50 |
| jrayhawk | In general I tend to avoid food that other people have processed on the basis that other people are terrible and untrustworthy, so my recommendation is to learn to prepare foods. | 22:50 |
| jrayhawk | You can throw a dozen eggs into a pot of boiling water for 30-60 minutes and wind up with a lot of food for very little effort, for instance. | 22:51 |
| justanotheruser | jrayhawk: but it's what plants crave | 22:52 |
| jrayhawk | (Though eggs are kinda crap for general nutrition due to mineral- and vitamin-binding antinutrients) | 22:52 |
| brownies | yeah, that's not an unreasonable approach | 22:52 |
| brownies | i'm just trying to decouple getting decent nutrition from having to learn to cook | 22:52 |
| jrayhawk | Not going to happen in the current food environment unless you have someone you can trust to follow directions. | 22:53 |
| kanzure | see "other people are terrible and untrustworthy" | 22:53 |
| brownies | can't i just buy some sort of powdered food and mix with water? why does eating have to be so complex? | 22:55 |
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| venturecommunist | jrayhawk: the way i heard it you could get a vitamin b defficiency if you ate only the egg whites but that it would take a LOT of whites | 22:57 |
| venturecommunist | and even mixing in a little yolk would fix it | 22:57 |
| brownies | if i grudgingly cook eggs, will i need to cook other things too? | 22:58 |
| jrayhawk | brownies: Two thoughts on that: 1) not all chemicals can survive free-floating with a wide variety of other chemicals, and 2) you're not eating for one, you're eating for 10 trillion | 22:59 |
| jrayhawk | the latter of which is to say even if overabsorbability didn't have consequences inside your circulation, it has some pretty significant ones outside of it | 22:59 |
| brownies | hm, i see | 22:59 |
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| jrayhawk | venturecommunist: The B vitamin absorbtion inhibitor I'm halfway familiar with is avidin, but there's anomalously low bioavailability of others when measured in the science literature. | 23:01 |
| jrayhawk | And similarly conalbumin for minerals. | 23:01 |
| jrayhawk | Avidin is at least fairly easy to deactivate through heavy boiling. | 23:01 |
| jrayhawk | Anyway, John Kiefer and Martin Berkhan are good fun to read for basic macronutrient strategies and link to a lot of interesting science. | 23:02 |
| brownies | ah, that's what i needed | 23:03 |
| jrayhawk | Chris Kresser has a lot of really amazingly good resources on a lot of nutrition biochem minutiae. | 23:04 |
| jrayhawk | Mark Sisson's fun for more general lifestyle stuff. | 23:05 |
| jrayhawk | http://www.marksdailyapple.com/is-barbell-dogma-doing-more-harm-than-good/ like this article | 23:19 |
| jrayhawk | it is a good article | 23:19 |
| jrayhawk | https://secure.piny.be/cgit/jrayhawk/tree/rss.mdwn there's other random stuff under the "health" category that might strike your fancy | 23:31 |
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| jrayhawk | http://www.youtube.com/watch?v=NqwvcrA7oe8 is a pretty good example of what fatty acid metabolic adaptation can do if you're into endurance athletics | 23:33 |
| venturecommunist | jrayhawk: checking it out, cool | 23:44 |
| brownies | nice. thanks. | 23:46 |
| --- Log closed Tue Jan 14 00:00:23 2014 | ||
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