| --- Log opened Wed Jan 15 00:00:24 2014 | ||
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| venturecommunist | this looks useful http://www.clothocad.org/ | 03:23 |
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| @archels | http://aisb50.org/ | 04:32 |
| @archels | anyone going to this? | 04:32 |
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| superkuh | paperbot: http://www.nature.com/neuro/journal/vaop/ncurrent/full/nn.3620.html | 07:45 |
| paperbot | http://libgen.org/scimag/get.php?doi=10.1038%2Fnn.3620 | 07:45 |
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| solom | hi | 08:36 |
| solom | What is the low hanging fruit of hplus? | 08:36 |
| kanzure | programming | 08:36 |
| kanzure | basic lab skills, like running a pipetting machine or spectrogramscopegraph | 08:38 |
| solom | I was asking about things I can apply to myself | 08:38 |
| solom | that are proven to work with most people | 08:38 |
| kanzure | learning skills is the most effective way to make transhumanism-related technology happen | 08:39 |
| kanzure | http://diyhpl.us/wiki/declaration/ | 08:39 |
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| solom | kanzure, http://diyhpl.us/wiki/nootropics/notes.txt | 08:48 |
| solom | What nootropics do you personally use | 08:49 |
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| solom | http://diyhpl.us/wiki/users/kanzure/bodybuilding-drugs/ | 08:50 |
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| ParahSailin | amphetamine is the most effective noot | 08:53 |
| solom | Aren't they a bit dangerous? | 08:54 |
| ThomasEgi | does gradstudent bot know about this? -> "Recently," Baker writes, "I learned of a governing principle known as Murphy's first law of biology. It states: 'Under any given set of environmental conditions an experimental animal behaves as it damn well pleases.' | 08:57 |
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| ParahSailin | only dangerous is you're the kind afraid of becoming more than human | 09:00 |
| chris_99 | you guys all seen the trailer for transcendance ? | 09:00 |
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| wallmani | ParahSailin: do have hany any recommendations on what amphetamines to use? | 09:23 |
| ParahSailin | whatever you can get your hands on | 09:24 |
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| wallmani | any non prescription ones i can get? | 09:25 |
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| ParahSailin | have you considered bath salts? | 09:28 |
| chris_99 | ThomasEgi, got my transducers, and just ordered a sig gen :) | 09:29 |
| ParahSailin | specifically, 4-fluoroamphetamine | 09:36 |
| cthulu | you can order stuff from hong kong | 10:32 |
| cthulu | modafinil, dexamphetamine | 10:32 |
| wallmani | cthulu: any recommended vendors for dexamphetamine? | 10:39 |
| wallmani | ParahSailin: aren't bath salts bad? | 10:39 |
| ua | wallmani, "thats what they want you to think" | 10:41 |
| ua | but yea if you dont know what youre doing its just like with everything | 10:42 |
| wallmani | ah, i see | 10:42 |
| cthulu | go to #reddit-nootropics for sources | 10:44 |
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| kanzure | no reddit | 10:55 |
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| ParahSailin | wallmani: but seriously if you are trying to be cheap about it, ethylphenidate is probably cheaper than even an indian pharmacy for ritalin | 11:17 |
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| wallmani | i see | 11:52 |
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| kanzure | this place has a very small selection of lab supplies but they claim to accept bitcoin http://www.geneandcell.com/ | 15:59 |
| kanzure | oh, it's john schloendorn... | 15:59 |
| kanzure | well that's boring | 16:00 |
| kanzure | i thought he was running that other molecular biology supply company too | 16:05 |
| kanzure | hrmm i guess it doesn't say http://www.openbiotech.com/aboutus.asp | 16:05 |
| kanzure | "Open Biotechnology, Inc. 3603 Haven Ave., Suite 1, Menlo Park, CA 94025" blargh who is it that lives here.. hrm.. | 16:06 |
| kanzure | Justin Rebo <justin@openbiotech.com> | 16:06 |
| kanzure | John Schloendorn <john@openbiotech.com> | 16:06 |
| kanzure | ParahSailin: what's the story about this.. john just happens to be doing the same thing under two names at the same time? | 16:07 |
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| ParahSailin | i never knew the guy very well | 16:27 |
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| kanzure | bummer. next time make something up and i'd be just as happy. | 16:36 |
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| kanzure | https://github.com/gulpjs/gulp "By harnassing the power of node's streams you get fast builds that don't write intermediary files to disk." | 20:00 |
| kanzure | show me more mad science | 20:00 |
| kanzure | i thought seaport/fleet would do continuous deployment but i'm not completely sure | 20:00 |
| kanzure | e.g. hooking up continuous builds to continuous deployment seems like a fun thing to do | 20:01 |
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| kanzure | nmz787: https://npmjs.org/package/inflate-stream for inflate/deflate in pdfs | 20:15 |
| kanzure | node stream visualization http://thlorenz.github.io/stream-viz/ | 20:24 |
| * juri_ waits to hear from the police whether her favorite transhumanist just attempted suicide while on the phone with her or not. | 21:22 | |
| juri_ | yep. things are great in my world. | 21:22 |
| kanzure | if you tell me the location i might have people nearby | 21:22 |
| juri_ | boulder colerado. | 21:23 |
| juri_ | police have been dispatched. | 21:23 |
| juri_ | i've attempted suicide a half dozen times myself, so i don't fuck around. as they say in heart attack cases, time is tissue. | 21:25 |
| juri_ | as for why i'm someone's last call, i only chalk that up to the universe's sense of irony / being a bastard. this is not my first time. | 21:26 |
| kanzure | there's john english <john@boulderes.com> from the open source hardware scene, but i haven't seen him in a while | 21:27 |
| kanzure | is it amara? | 21:27 |
| juri_ | Free Hardware is a rough scene indeed. | 21:27 |
| juri_ | nope. | 21:27 |
| kanzure | yeah i think amara left boulder a while ago. hrmm. | 21:28 |
| kanzure | it's not like there's that many transhumanists anyway | 21:28 |
| juri_ | technically, i don't consider myself a transhumanist. i'm a singularist. | 21:29 |
| juri_ | now, don't ask me to define the difference. we could be here all night. ;) | 21:30 |
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| kanzure | oh geeze now i have to cross-reference all the singularitarians in boulder, all 5 of them or something | 21:31 |
| kanzure | hrmm | 21:31 |
| juri_ | singulitarians? they only eat things that come individually served? ;) | 21:31 |
| kanzure | no.. http://wiki.lesswrong.com/wiki/Singularitarianism | 21:32 |
| kanzure | hah "Yudkowsky has (perhaps facetiously) suggested that those adhering to the original activist stance relabel themselves the "Elder Singularitarians"." | 21:32 |
| kanzure | classic eliezer | 21:32 |
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| juri_ | wait, dr sbaetzo isn't a real doctor? ;) | 21:34 |
| gradstudentbot | Did you order the carbon nanotubes yet? | 21:34 |
| juri_ | No, i haven't. :( | 21:35 |
| kanzure | ./buildwrappers.sh: line 50: ed: command not found | 21:36 |
| kanzure | is it really possible that i don't have ed? | 21:37 |
| kanzure | seems unlikely. remaining skeptical. | 21:37 |
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| Urchin[emacs] | what are you using? | 21:39 |
| kanzure | building swig things for opentransactions | 21:42 |
| kanzure | apparently i did not have ed installed.. | 21:42 |
| venturecommunist | semweb interest group and financial crypto? | 21:46 |
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| kanzure | swig is a wrapper generator | 21:47 |
| kanzure | the semantic web stuff is unrelated and boring | 21:47 |
| venturecommunist | i've been working with financial crypto built on http://json-ld.org | 21:48 |
| venturecommunist | http://web-payments.org | 21:48 |
| kanzure | that's quite a lot of words | 21:49 |
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| venturecommunist | words as opposed to? | 21:50 |
| kanzure | specs, code, etc. | 21:50 |
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| kanzure | this does not seem very compelling https://web-payments.org/specs/source/web-commerce/ | 21:50 |
| kanzure | yeah let's have a pile of unsigned transactions in json.. perfect? | 21:50 |
| venturecommunist | signatures https://web-payments.org/specs/source/http-signatures/ | 21:51 |
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| venturecommunist | you found some specs clearly. here's some of my code https://github.com/venturecommunism/meteor-httpkeys/blob/master/server/httpsig.js | 21:54 |
| venturecommunist | so there's specs and code | 21:54 |
| kanzure | if(Meteor.isServer) {} | 21:56 |
| kanzure | is that really how meteor does server/client side separation | 21:56 |
| gradstudentbot | Oh man, that's a great scrabble word. I got to write that down. | 21:56 |
| venturecommunist | kanzure: i just keep server code in the server folder | 21:58 |
| juri_ | ok, friend is fine. | 21:58 |
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| venturecommunist | anyone have a dna assembly method they've used for fusion proteins that has minimal equipment requirements? | 22:08 |
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| kanzure | what's wrong with in vivo protein construction..? | 22:13 |
| venturecommunist | kanzure: you mean with no transformation step at all? | 22:14 |
| venturecommunist | i didn't know you could make a predictable fusion protein thatway | 22:14 |
| venturecommunist | or do you just mean "what's wrong with doing nothing" | 22:15 |
| kanzure | dna templated assembly just seems like a huge hack around just doing normal molecular biology techniques | 22:16 |
| kanzure | "let's use dna folding and stitches to make the proteins" is not something that biologists smile about every morning | 22:16 |
| venturecommunist | so are there specs and protocols for this? | 22:18 |
| venturecommunist | i don't know i've come across this yet on openwetware | 22:19 |
| kanzure | the dna origami-guided protein assembly stuff? there's probably a stupid paper somewhere | 22:19 |
| kanzure | openwetware is not a great way to learn about traditional molecular biology | 22:19 |
| kanzure | except maybe finding a protocol or two sometimes | 22:19 |
| kanzure | start here, http://diyhpl.us/~bryan/papers2/bio/books/Molecular%20Cloning%20-%20A%20Laboratory%20Manual%20-%20Sambrook%20-%203rd%20edition.pdf | 22:19 |
| venturecommunist | k thanks i'll read this | 22:22 |
| venturecommunist | kanzure: does this fall under DNA synthesis then? | 22:23 |
| venturecommunist | i don't know if that's a loose or specific term but you couldn't specify base pairs from scratch like this could you? | 22:24 |
| kanzure | you can sometimes use dna synthesis to write entire genes but often it's extremely expensive | 22:24 |
| kanzure | well, sorry, no, average gene size is short, so it's not often expensive, but the fun stuff is at least $5k/protein or something | 22:24 |
| venturecommunist | right which is why i haven't learned much about it | 22:24 |
| kanzure | http://diyhpl.us/wiki/dna-synthesis.html | 22:24 |
| venturecommunist | so this has transformation and plasmids in it too | 22:24 |
| venturecommunist | what makes molecular cloning and dna assembly distinct categories? | 22:25 |
| kanzure | are you talking about this sort of dna assembly? http://dna.caltech.edu/DNAresearch_publications.html | 22:26 |
| venturecommunist | that looks more advanced. i'm definitely talking miniprep, PCR, heat shock transformation, etc | 22:28 |
| venturecommunist | minimal equipment would be my ideal, with the goal of making fusion proteins | 22:28 |
| venturecommunist | specifically HIV1 TAT + PKR + APAF1 | 22:28 |
| kanzure | then why do you keep saying dna assembly.. what is that if it's not that stuff i linked to. | 22:28 |
| venturecommunist | i'm just learning the terminology, i've seen assembly used a lot | 22:29 |
| kanzure | for quickest results you can outsource to scienceexchange.com and then slowly bring parts of the process in-house as you cover more of it | 22:29 |
| venturecommunist | very cool, i think i'll do that | 22:30 |
| venturecommunist | kanzure: you think they would have a problem wth me requesting this, not being a lab? | 22:31 |
| venturecommunist | oh i connect with a lab first? ok | 22:31 |
| kanzure | yes i've heard anecdotes that they are not thrilled about it but fuck 'em | 22:31 |
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| venturecommunist | kanzure: this thing that's called amplified insert cause you amplify the insert plasmid in a PCR, it's highly do-able except for the purification step | 22:33 |
| venturecommunist | what i mean is that i have uncertainty re: purification | 22:34 |
| venturecommunist | it mentions a dna binding column several times as if it's an advantage / low requirement | 22:34 |
| kanzure | purification happens with liquid chromatography columns | 22:34 |
| venturecommunist | the original paper says "dna binding column" not sure if that's the same? | 22:35 |
| kanzure | why don't i just buy the equipment you need.. what are you doing again? i hvaen't looked. | 22:35 |
| venturecommunist | it's a broad spectrum anti-viral therapeutic | 22:36 |
| venturecommunist | based on a fusion protein expressed in a bacterium so i assume e coli | 22:36 |
| venturecommunist | it's effective in vitro on 15 viruses | 22:36 |
| venturecommunist | 2 in vivo mouse models | 22:36 |
| kanzure | some types of proteins can be made in vitro | 22:37 |
| venturecommunist | domain 1 is HIV1 TAT usually, but the concept is to pass the blood brain barrier, get into cells and my own theory is that it would evade HIV1 | 22:37 |
| venturecommunist | domain 2 is PKR which binds double stranded RNA and initiates apoptosis via a lengthy process nearly all viruses actively subvert | 22:38 |
| venturecommunist | APAF1 induces apoptosis very immediately, by dimerizing with itself when multiple DRACOs attach to some dsRNA | 22:38 |
| venturecommunist | that causes caspase-9 to dimerize which induces very quick apoptosis | 22:39 |
| venturecommunist | so it's called dsRNA activated caspase oligomerizer | 22:40 |
| venturecommunist | (draco) | 22:40 |
| venturecommunist | kanzure: i don't think you'd want to make free HIV1 TAT in vitro | 22:44 |
| venturecommunist | vivo* | 22:44 |
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| venturecommunist | some theories going around that free TAT could be causal in Alzheimer's | 22:45 |
| venturecommunist | not that there's a human gene for making it? | 22:45 |
| venturecommunist | not sure how this all works | 22:45 |
| venturecommunist | PKR and APAF1 are human | 22:45 |
| kanzure | unrelated, | 22:51 |
| kanzure | target malware http://krebsonsecurity.com/2014/01/a-first-look-at-the-target-intrusion-malware/ | 22:51 |
| kanzure | fenn: ted chiang things for you, http://www.nature.com/nature/journal/v436/n7047/full/436150a.html | 22:51 |
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| venturecommunist | kanzure: originally i was thinking "column" meant spin column so i was worried | 23:11 |
| venturecommunist | now i'm thinking maybe it means a kit like this https://www.lifetechnologies.com/us/en/home/references/protocols/nucleic-acid-purification-and-analysis/dna-extraction-protocols/purelink-hipure-plasmid-filter-purification-kits.html | 23:12 |
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