2014-03-29.log

--- Log opened Sat Mar 29 00:00:17 2014
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kanzure06:16 < ThickAsThieves> or like the people that are most likely to say "everyone is entitled to their own opinion" are the least likely to have opinions worth considering?07:00
kanzurethis guy seems ok07:00
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ParahSailinanyone know the deal with these guys? http://www.bulldog-bio.com/07:28
ParahSailintheyve got pretty cheap reagents, and even some lyophilized no-freeze pcr master mix07:28
ParahSailinid wondered why nobody sold master mix like that07:28
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delinquentmekanzure, paperbot iz sick =[11:33
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kanzureportable shellcode using signal frames http://www.cs.vu.nl/~herbertb/papers/srop_sp14.pdf11:47
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delinquentmepaperbot, http://www.sciencemag.org.sci-hub.org/content/343/6173/868.short11:59
delinquentmepaperbot = jesus11:59
paperbothttp://libgen.org/scimag/get.php?doi=10.1126%2Fscience.124690611:59
delinquentmepaperbot, that is not a functioning paper!!!!12:10
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ParahSailinif only there were a way for interested parties to peek into the internals and poke around until it works12:33
kanzurewow that's an innovative concept12:34
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ParahSailinweb audio api is kind of sad12:49
ParahSailinfft should not be so slow12:50
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delinquentmepaperbot, http://onlinelibrary.wiley.com/doi/10.1002/adma.200801396/abstract14:08
paperbothttp://diyhpl.us/~bryan/papers2/paperbot/dab20a0cf8458983a839470158237f0d.txt14:08
delinquentmekanzure, ParahSailin hows the relationship going?14:08
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kanzureyawn14:09
kanzurei want google scholar image search14:15
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kanzureyashgaroth: do you have a buy-out price14:58
yashgarothlike per year or just total14:59
kanzureto get out of the startup14:59
kanzureand to be doing other things instead14:59
yashgarothwait what startup14:59
kanzureoh i thought two people recruited you into this two-man, er three-man startup somewhere near san diego15:00
yashgarothwell I have an ex-coworker at a two-person in janssen labs who promises a job when they have need for a person, but my current place is 20,000 people15:02
kanzureoh! i was not aware of this.15:02
kanzurethat makes it sound like you're underemployed or wrong-employed15:02
yashgarothwell the pay's a little crap but that's biotech - like and large and GMP facility, they do throw out a whole lot of expensive shit so that's a bonus at least15:03
yashgarotha large*15:03
kanzure"there's lots of garbage here, so that's nice"15:03
yashgarothgarbage that costs more than gold by weight, but yeah15:03
kanzuresomeone was wondering what equipment jojack has at his lab15:04
kanzureand i don't know15:04
kanzurewas wondering if you remember15:04
yashgaroththey got a bunch after orphi or whoever moved in, they actually had a lab and just moved it all in15:04
yashgarothnow he's actually turning down equipment15:05
kanzurethe one that just moved in is sharing the equipment among the other occupants?15:05
yashgarothoccupants as in other paying companies, I believe so yes15:05
juri_kanzure: we're installing an SEM in HacDC. a 'stereoscan 200'.15:11
juri_my plan is to use it to verify the DLC coatings, when i build the DLC coating machine.15:11
kanzurewhy are the colors for neurophysiology so similar to the colors for molecular biology? how did that happen15:18
kanzurethe colors of other major branches aren't so matched. like plant biology.15:18
kanzuremaybe it's just all the same suppliers15:19
yashgarothcolors?15:20
kanzureyep all the containers, gloves, pipettes, etc.15:20
kanzureyou walk into a neuro lab and you have to look very close to find out it's the wrong lab15:21
kanzuresometimes more tinfoil, which is a giveaway15:21
yashgarothwell most of that stuff is shareable between departments, and anyway it all comes from Fisher15:25
yashgarothI suppose plant biologists have trowels and such15:25
kanzureheh thermo fisher trowels15:25
yashgaroth$30015:26
kanzureiirc they looked like they were from a garden shop15:26
kanzurebut i never thought about it15:26
kanzureprobably from a supplier, yeah15:26
yashgarothplenty of stuff in R&D labs is store bought, non-fat milk powder makes a great blocker for westerns15:26
yashgarothplant bio is a little more forgiving of contamination, incubation times etc15:27
kanzurethus my interest in agrobacterium for transfection15:28
kanzurestill can't believe superkuh is the only one who bothered to go hunting for some15:29
kanzureand that was years prior to diybio15:29
yashgarothagrobacterium transfection protocol, step 7: just kinda rub it on the plant, get it all in there15:30
kanzurei wonder if lancing the plant would help15:30
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yashgarothheh I thought I was exaggerating but "Dip above-ground parts of plant in Agrobacterium solution for 2 to 3 seconds" apparently...though yes normally if you want to be efficient you mix it with plant cells and plate them out15:31
kanzureoh, actually i've always thought more in terms of hairy root culture or something, instead of plated plant cell cultures15:32
kanzure"A modified Ti or Ri plasmid can be used. The plasmid is 'disarmed' by deletion of the tumor inducing genes; the only essential parts of the T-DNA are its two small (25 base pair) border repeats, at least one of which is needed for plant transformation. Marc Van Montagu and Jozef Schell at the University of Ghent (Belgium) discovered the gene transfer mechanism between Agrobacterium and plants, which resulted in the development of methods to ...15:32
kanzure... alter Agrobacterium into an efficient delivery system for gene engineering in plants.[7][8] A team of researchers led by Dr Mary-Dell Chilton were the first to demonstrate that the virulence genes could be removed without adversely affecting the ability of Agrobacterium to insert its own DNA into the plant genome (1983)."15:32
kanzureoh i see how it is "Along with his colleague, Marc Van Montagu, Jeff Schell discovered the gene transfer mechanism between Agrobacterium and plants, which resulted in the development of methods to alter Agrobacterium into an efficient delivery system for gene engineering in plants. Besides being an excellent scientist, he founded in collaboration with Marc Van Montagu the successful biotech company Plant Genetic Systems Inc., Belgium in 1982, ...15:33
kanzure... now part of Bayer CropScience."15:33
yashgaroththere's the advantage of using antibiotic selection if you plate cells, otherwise you're waiting for seeds to re-plant and confirm15:34
yashgarothgetting miraculin expression/activity in a. thaliana would be a fun experiment, been meaning to look into that15:38
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Lemminkainenpaperbot http://www.nature.com/nature/journal/v507/n7493/full/nature12998.html16:45
paperbothttp://libgen.org/scimag/get.php?doi=10.1038%2Fnature1299816:45
ParahSailinkanzure: why is this working in firefox but not chrome? what is the magic invokation http://jsfiddle.net/Q9ZF5/2/16:57
kanzureuh does chrome have the html5 audio api implemented yet?16:58
ParahSailinit does, i can make analyser node and do fft on it16:58
ParahSailinbut this script node i guess is not implemented properly yet?16:58
kanzureyou could check the chromium source code pretty easily17:00
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ParahSailini get the feeling im gonna have to play with swf shit17:02
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ParahSailinseemingly in webkit https://github.com/WebKit/webkit/blob/8fdbf242ac805fe4690081c7ccd2f5052b1a4719/Source/WebCore/Modules/webaudio/ScriptProcessorNode.cpp17:22
kanzurechrome forked into blink, so you have to check blink instead of webkit unfortunately17:22
ParahSailinlooks pretty much the same https://github.com/mirrors/blink/blob/b1bbf091a65a3a38b4f28af67da171198a0048ee/Source/modules/webaudio/ScriptProcessorNode.cpp17:25
* FourFire is drunk and would probbbblyl only make a mess17:26
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ParahSailinah "ScriptProcesser's onaudioprocess event will not start if its output is not connected to some other node." chrome peculiarity17:27
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kanzuresomeone should hook up an ardrone to an oculus18:52
kanzurehttp://digitalinterface.blogspot.com/2014/03/strangecoin-proposal-for-nonlinear.html18:59
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kanzurei'm issuing shares on the bitcoin blockchain22:35
kanzureif you would like some then tell me a bitcoin address to send to22:35
kanzuremust be an address that you control the private key to (some web wallets don't tell you this information etc)22:36
kanzurehttp://bitaddress.org/ or http://brainwallet.org/ just make sure to write down both the public and private key22:36
caternshares of what?22:49
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kanzurejust me.22:51
caterndo these shares pay dividends or have voting rights?22:51
kanzuredividends22:51
kanzureno voting rights22:51
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caternyou're selling them? or giving them away for free?22:52
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kanzureat the moment i am giving them away22:53
caternfor what purpose22:53
kanzuresinister plot?22:53
caternoh, ok22:53
kanzuresinister plot to give things away for free22:53
kanzurewatch out22:53
caternindeed22:53
-!- AshleyWaffle [~quassel@unaffiliated/anastasiawyatt] has joined ##hplusroadmap22:54
AshleyWafflehello22:54
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AshleyWafflehttps://www.youtube.com/watch?v=xxvC5Qh_j1g22:56
AshleyWaffle^music22:56
AshleyWafflealso if anyone is interested in a more casual channel, we are looking to grow our transhumanist population in ##austrians. it's a 40+ person cool channel run by I, ParahSailin, and others22:57
AshleyWafflebe forewarned, it may not be super quality, but it's still growing and improving, and you won't be banned for anything less than malicious trolling/spam22:58
AshleyWafflehi, I just joined. could I have the last 100 lines in a pastebin so I can see if there's something recent I want to discuss? thanks23:04
kanzurehttp://gnusha.org/logs/2014-03-29.log23:05
AshleyWafflety23:05
AshleyWaffleout of curiousity...23:08
AshleyWafflekanzure: how old are you?23:09
kanzureas ancient as time itself23:09
AshleyWafflelol23:09
kanzureso approximately 24 years23:09
AshleyWaffleokay23:09
AshleyWaffleso I'm 18 now23:09
AshleyWaffleI'm going to guess that unless you were unschooled, or homeschooled really well, or went to some crazy private school, you probably knew as much as me when you were 1823:10
rigeljesus, i have been ircing roughly as long as you chumps have been alive23:10
AshleyWafflewhich is to say, a lot of really cool ideas like nanotech and stuff are known to you23:10
rigelcertanly longer than AshleyWaffle and almost as long as kanzure23:10
AshleyWaffleso here's my question: how the fuck does someone *LEARN* all that in like 6 years?23:10
AshleyWafflerigel: hah nice23:10
kanzurei went to a crazy private school called waldorf but they kicked me out23:11
ParahSailini remember that report card you showed me23:11
AshleyWafflelike I know the names and basic ideas of a lot of really cool leading edge ideas23:11
ParahSailinweird touchy feely stuff23:11
AshleyWafflebut how does someone LEARN THAT STUFF23:11
AshleyWaffleParahSailin: me?23:11
ParahSailinno23:12
rigelwhat the fuck do you mean "leading edge ideas"?23:12
kanzureoh yeah, http://diyhpl.us/~bryan/waldorf/stuff.txt23:12
kanzurerigel: yeah..23:12
AshleyWafflerigel: things that are more likely to have an effect on transhumanism and the singularity than other ideas23:12
kanzureAshleyWaffle: nanotech is not cool it is boring because it doesn't work23:12
AshleyWafflefor example, nanotech (or not), BCIs, nootropics, etc23:12
AshleyWaffleAGI23:12
rigelwho gives a shit about transhumanism and "the singularity"?23:12
rigelis that what most of us are in here for?23:13
kanzureyes23:13
AshleyWaffleI think so yeah23:13
rigeli thought it was to make fun of ray kurzweil23:13
AshleyWaffleI kinda want to live forever and become a goddess23:13
AshleyWafflelol...23:13
AshleyWafflerigel: that time of the month?23:13
kanzurebetter start reading up then http://diyhpl.us/~bryan/papers2/longevity/23:13
rigelAshleyWaffle: i am nearly 40. this shit is all laughable white middle class fables23:13
AshleyWafflekanzure: but how does anyone LEARN this stuff23:13
kanzuredrugs23:14
AshleyWafflein such a short period of time23:14
kanzurebrain damage23:14
AshleyWafflerigel: that's pretty cool *back to actually interesting conversation*23:14
rigelwhat do you mean "learn"? it's all about the grift, like aubrey de grey's immortality grift23:14
AshleyWafflekanzure: I thought nootropics were only minimally effective23:14
AshleyWafflegrift?23:14
rigelhe was able to convince a billionaire to give him a ton of money because well *handwave*23:15
AshleyWafflehaha23:15
kanzurenah that's not quite aubrey's funding story23:15
kanzureaubrey is loaded23:15
rigels/grift/scam/ if you like23:15
AshleyWaffleit's just that this place is alien to me23:15
AshleyWaffleI'm pretty sure the level and diversity of knowledge in here rivals Einstein23:15
kanzurerigel: do you think that lysosomal damage is a scam?23:15
AshleyWaffleif not more23:15
kanzureuh.. why would einstein be a measure of diversity of knowledge23:16
AshleyWaffleyou know what I mean23:16
AshleyWaffleit's like23:16
kanzureno i actually don't23:16
rigelkanzure: i am quite certain that the people selling it for money are scammers, regardless of their belief in it or some scant data that suggest OMG THIS IS GREAT23:16
kanzureselling what for money?23:16
AshleyWafflethere's normal people, then scientists, then this sort of place23:16
kanzurethere's no lysosomal therapies on the market23:16
rigeloh, noone's selling gray market therapies?23:17
kanzureAshleyWaffle: http://diyhpl.us/wiki/declaration23:17
AshleyWafflewhat is lysosomal therapy23:17
kanzureAshleyWaffle: http://diyhpl.us/~bryan/papers2/longevity/Aubrey/23:17
rigeloh ffs23:17
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kanzureit's not often that people are upset that i don't type fast enough23:18
kanzurepeople are getting picky these days23:18
ParahSailinhaha23:18
AshleyWafflehmm23:19
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jrayhawkthe more i learn about biochemistry and human health, the more grifty aubrey sounds to me23:58
--- Log closed Sun Mar 30 00:00:18 2014

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