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kanzure | 06:16 < ThickAsThieves> or like the people that are most likely to say "everyone is entitled to their own opinion" are the least likely to have opinions worth considering? | 07:00 |
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kanzure | this guy seems ok | 07:00 |
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ParahSailin | anyone know the deal with these guys? http://www.bulldog-bio.com/ | 07:28 |
ParahSailin | theyve got pretty cheap reagents, and even some lyophilized no-freeze pcr master mix | 07:28 |
ParahSailin | id wondered why nobody sold master mix like that | 07:28 |
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delinquentme | kanzure, paperbot iz sick =[ | 11:33 |
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kanzure | portable shellcode using signal frames http://www.cs.vu.nl/~herbertb/papers/srop_sp14.pdf | 11:47 |
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delinquentme | paperbot, http://www.sciencemag.org.sci-hub.org/content/343/6173/868.short | 11:59 |
delinquentme | paperbot = jesus | 11:59 |
paperbot | http://libgen.org/scimag/get.php?doi=10.1126%2Fscience.1246906 | 11:59 |
delinquentme | paperbot, that is not a functioning paper!!!! | 12:10 |
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ParahSailin | if only there were a way for interested parties to peek into the internals and poke around until it works | 12:33 |
kanzure | wow that's an innovative concept | 12:34 |
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ParahSailin | web audio api is kind of sad | 12:49 |
ParahSailin | fft should not be so slow | 12:50 |
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delinquentme | paperbot, http://onlinelibrary.wiley.com/doi/10.1002/adma.200801396/abstract | 14:08 |
paperbot | http://diyhpl.us/~bryan/papers2/paperbot/dab20a0cf8458983a839470158237f0d.txt | 14:08 |
delinquentme | kanzure, ParahSailin hows the relationship going? | 14:08 |
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kanzure | yawn | 14:09 |
kanzure | i want google scholar image search | 14:15 |
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kanzure | yashgaroth: do you have a buy-out price | 14:58 |
yashgaroth | like per year or just total | 14:59 |
kanzure | to get out of the startup | 14:59 |
kanzure | and to be doing other things instead | 14:59 |
yashgaroth | wait what startup | 14:59 |
kanzure | oh i thought two people recruited you into this two-man, er three-man startup somewhere near san diego | 15:00 |
yashgaroth | well I have an ex-coworker at a two-person in janssen labs who promises a job when they have need for a person, but my current place is 20,000 people | 15:02 |
kanzure | oh! i was not aware of this. | 15:02 |
kanzure | that makes it sound like you're underemployed or wrong-employed | 15:02 |
yashgaroth | well the pay's a little crap but that's biotech - like and large and GMP facility, they do throw out a whole lot of expensive shit so that's a bonus at least | 15:03 |
yashgaroth | a large* | 15:03 |
kanzure | "there's lots of garbage here, so that's nice" | 15:03 |
yashgaroth | garbage that costs more than gold by weight, but yeah | 15:03 |
kanzure | someone was wondering what equipment jojack has at his lab | 15:04 |
kanzure | and i don't know | 15:04 |
kanzure | was wondering if you remember | 15:04 |
yashgaroth | they got a bunch after orphi or whoever moved in, they actually had a lab and just moved it all in | 15:04 |
yashgaroth | now he's actually turning down equipment | 15:05 |
kanzure | the one that just moved in is sharing the equipment among the other occupants? | 15:05 |
yashgaroth | occupants as in other paying companies, I believe so yes | 15:05 |
juri_ | kanzure: we're installing an SEM in HacDC. a 'stereoscan 200'. | 15:11 |
juri_ | my plan is to use it to verify the DLC coatings, when i build the DLC coating machine. | 15:11 |
kanzure | why are the colors for neurophysiology so similar to the colors for molecular biology? how did that happen | 15:18 |
kanzure | the colors of other major branches aren't so matched. like plant biology. | 15:18 |
kanzure | maybe it's just all the same suppliers | 15:19 |
yashgaroth | colors? | 15:20 |
kanzure | yep all the containers, gloves, pipettes, etc. | 15:20 |
kanzure | you walk into a neuro lab and you have to look very close to find out it's the wrong lab | 15:21 |
kanzure | sometimes more tinfoil, which is a giveaway | 15:21 |
yashgaroth | well most of that stuff is shareable between departments, and anyway it all comes from Fisher | 15:25 |
yashgaroth | I suppose plant biologists have trowels and such | 15:25 |
kanzure | heh thermo fisher trowels | 15:25 |
yashgaroth | $300 | 15:26 |
kanzure | iirc they looked like they were from a garden shop | 15:26 |
kanzure | but i never thought about it | 15:26 |
kanzure | probably from a supplier, yeah | 15:26 |
yashgaroth | plenty of stuff in R&D labs is store bought, non-fat milk powder makes a great blocker for westerns | 15:26 |
yashgaroth | plant bio is a little more forgiving of contamination, incubation times etc | 15:27 |
kanzure | thus my interest in agrobacterium for transfection | 15:28 |
kanzure | still can't believe superkuh is the only one who bothered to go hunting for some | 15:29 |
kanzure | and that was years prior to diybio | 15:29 |
yashgaroth | agrobacterium transfection protocol, step 7: just kinda rub it on the plant, get it all in there | 15:30 |
kanzure | i wonder if lancing the plant would help | 15:30 |
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yashgaroth | heh I thought I was exaggerating but "Dip above-ground parts of plant in Agrobacterium solution for 2 to 3 seconds" apparently...though yes normally if you want to be efficient you mix it with plant cells and plate them out | 15:31 |
kanzure | oh, actually i've always thought more in terms of hairy root culture or something, instead of plated plant cell cultures | 15:32 |
kanzure | "A modified Ti or Ri plasmid can be used. The plasmid is 'disarmed' by deletion of the tumor inducing genes; the only essential parts of the T-DNA are its two small (25 base pair) border repeats, at least one of which is needed for plant transformation. Marc Van Montagu and Jozef Schell at the University of Ghent (Belgium) discovered the gene transfer mechanism between Agrobacterium and plants, which resulted in the development of methods to ... | 15:32 |
kanzure | ... alter Agrobacterium into an efficient delivery system for gene engineering in plants.[7][8] A team of researchers led by Dr Mary-Dell Chilton were the first to demonstrate that the virulence genes could be removed without adversely affecting the ability of Agrobacterium to insert its own DNA into the plant genome (1983)." | 15:32 |
kanzure | oh i see how it is "Along with his colleague, Marc Van Montagu, Jeff Schell discovered the gene transfer mechanism between Agrobacterium and plants, which resulted in the development of methods to alter Agrobacterium into an efficient delivery system for gene engineering in plants. Besides being an excellent scientist, he founded in collaboration with Marc Van Montagu the successful biotech company Plant Genetic Systems Inc., Belgium in 1982, ... | 15:33 |
kanzure | ... now part of Bayer CropScience." | 15:33 |
yashgaroth | there's the advantage of using antibiotic selection if you plate cells, otherwise you're waiting for seeds to re-plant and confirm | 15:34 |
yashgaroth | getting miraculin expression/activity in a. thaliana would be a fun experiment, been meaning to look into that | 15:38 |
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Lemminkainen | paperbot http://www.nature.com/nature/journal/v507/n7493/full/nature12998.html | 16:45 |
paperbot | http://libgen.org/scimag/get.php?doi=10.1038%2Fnature12998 | 16:45 |
ParahSailin | kanzure: why is this working in firefox but not chrome? what is the magic invokation http://jsfiddle.net/Q9ZF5/2/ | 16:57 |
kanzure | uh does chrome have the html5 audio api implemented yet? | 16:58 |
ParahSailin | it does, i can make analyser node and do fft on it | 16:58 |
ParahSailin | but this script node i guess is not implemented properly yet? | 16:58 |
kanzure | you could check the chromium source code pretty easily | 17:00 |
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ParahSailin | i get the feeling im gonna have to play with swf shit | 17:02 |
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ParahSailin | seemingly in webkit https://github.com/WebKit/webkit/blob/8fdbf242ac805fe4690081c7ccd2f5052b1a4719/Source/WebCore/Modules/webaudio/ScriptProcessorNode.cpp | 17:22 |
kanzure | chrome forked into blink, so you have to check blink instead of webkit unfortunately | 17:22 |
ParahSailin | looks pretty much the same https://github.com/mirrors/blink/blob/b1bbf091a65a3a38b4f28af67da171198a0048ee/Source/modules/webaudio/ScriptProcessorNode.cpp | 17:25 |
* FourFire is drunk and would probbbblyl only make a mess | 17:26 | |
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ParahSailin | ah "ScriptProcesser's onaudioprocess event will not start if its output is not connected to some other node." chrome peculiarity | 17:27 |
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kanzure | someone should hook up an ardrone to an oculus | 18:52 |
kanzure | http://digitalinterface.blogspot.com/2014/03/strangecoin-proposal-for-nonlinear.html | 18:59 |
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kanzure | i'm issuing shares on the bitcoin blockchain | 22:35 |
kanzure | if you would like some then tell me a bitcoin address to send to | 22:35 |
kanzure | must be an address that you control the private key to (some web wallets don't tell you this information etc) | 22:36 |
kanzure | http://bitaddress.org/ or http://brainwallet.org/ just make sure to write down both the public and private key | 22:36 |
catern | shares of what? | 22:49 |
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kanzure | just me. | 22:51 |
catern | do these shares pay dividends or have voting rights? | 22:51 |
kanzure | dividends | 22:51 |
kanzure | no voting rights | 22:51 |
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catern | you're selling them? or giving them away for free? | 22:52 |
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kanzure | at the moment i am giving them away | 22:53 |
catern | for what purpose | 22:53 |
kanzure | sinister plot? | 22:53 |
catern | oh, ok | 22:53 |
kanzure | sinister plot to give things away for free | 22:53 |
kanzure | watch out | 22:53 |
catern | indeed | 22:53 |
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AshleyWaffle | hello | 22:54 |
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AshleyWaffle | https://www.youtube.com/watch?v=xxvC5Qh_j1g | 22:56 |
AshleyWaffle | ^music | 22:56 |
AshleyWaffle | also if anyone is interested in a more casual channel, we are looking to grow our transhumanist population in ##austrians. it's a 40+ person cool channel run by I, ParahSailin, and others | 22:57 |
AshleyWaffle | be forewarned, it may not be super quality, but it's still growing and improving, and you won't be banned for anything less than malicious trolling/spam | 22:58 |
AshleyWaffle | hi, I just joined. could I have the last 100 lines in a pastebin so I can see if there's something recent I want to discuss? thanks | 23:04 |
kanzure | http://gnusha.org/logs/2014-03-29.log | 23:05 |
AshleyWaffle | ty | 23:05 |
AshleyWaffle | out of curiousity... | 23:08 |
AshleyWaffle | kanzure: how old are you? | 23:09 |
kanzure | as ancient as time itself | 23:09 |
AshleyWaffle | lol | 23:09 |
kanzure | so approximately 24 years | 23:09 |
AshleyWaffle | okay | 23:09 |
AshleyWaffle | so I'm 18 now | 23:09 |
AshleyWaffle | I'm going to guess that unless you were unschooled, or homeschooled really well, or went to some crazy private school, you probably knew as much as me when you were 18 | 23:10 |
rigel | jesus, i have been ircing roughly as long as you chumps have been alive | 23:10 |
AshleyWaffle | which is to say, a lot of really cool ideas like nanotech and stuff are known to you | 23:10 |
rigel | certanly longer than AshleyWaffle and almost as long as kanzure | 23:10 |
AshleyWaffle | so here's my question: how the fuck does someone *LEARN* all that in like 6 years? | 23:10 |
AshleyWaffle | rigel: hah nice | 23:10 |
kanzure | i went to a crazy private school called waldorf but they kicked me out | 23:11 |
ParahSailin | i remember that report card you showed me | 23:11 |
AshleyWaffle | like I know the names and basic ideas of a lot of really cool leading edge ideas | 23:11 |
ParahSailin | weird touchy feely stuff | 23:11 |
AshleyWaffle | but how does someone LEARN THAT STUFF | 23:11 |
AshleyWaffle | ParahSailin: me? | 23:11 |
ParahSailin | no | 23:12 |
rigel | what the fuck do you mean "leading edge ideas"? | 23:12 |
kanzure | oh yeah, http://diyhpl.us/~bryan/waldorf/stuff.txt | 23:12 |
kanzure | rigel: yeah.. | 23:12 |
AshleyWaffle | rigel: things that are more likely to have an effect on transhumanism and the singularity than other ideas | 23:12 |
kanzure | AshleyWaffle: nanotech is not cool it is boring because it doesn't work | 23:12 |
AshleyWaffle | for example, nanotech (or not), BCIs, nootropics, etc | 23:12 |
AshleyWaffle | AGI | 23:12 |
rigel | who gives a shit about transhumanism and "the singularity"? | 23:12 |
rigel | is that what most of us are in here for? | 23:13 |
kanzure | yes | 23:13 |
AshleyWaffle | I think so yeah | 23:13 |
rigel | i thought it was to make fun of ray kurzweil | 23:13 |
AshleyWaffle | I kinda want to live forever and become a goddess | 23:13 |
AshleyWaffle | lol... | 23:13 |
AshleyWaffle | rigel: that time of the month? | 23:13 |
kanzure | better start reading up then http://diyhpl.us/~bryan/papers2/longevity/ | 23:13 |
rigel | AshleyWaffle: i am nearly 40. this shit is all laughable white middle class fables | 23:13 |
AshleyWaffle | kanzure: but how does anyone LEARN this stuff | 23:13 |
kanzure | drugs | 23:14 |
AshleyWaffle | in such a short period of time | 23:14 |
kanzure | brain damage | 23:14 |
AshleyWaffle | rigel: that's pretty cool *back to actually interesting conversation* | 23:14 |
rigel | what do you mean "learn"? it's all about the grift, like aubrey de grey's immortality grift | 23:14 |
AshleyWaffle | kanzure: I thought nootropics were only minimally effective | 23:14 |
AshleyWaffle | grift? | 23:14 |
rigel | he was able to convince a billionaire to give him a ton of money because well *handwave* | 23:15 |
AshleyWaffle | haha | 23:15 |
kanzure | nah that's not quite aubrey's funding story | 23:15 |
kanzure | aubrey is loaded | 23:15 |
rigel | s/grift/scam/ if you like | 23:15 |
AshleyWaffle | it's just that this place is alien to me | 23:15 |
AshleyWaffle | I'm pretty sure the level and diversity of knowledge in here rivals Einstein | 23:15 |
kanzure | rigel: do you think that lysosomal damage is a scam? | 23:15 |
AshleyWaffle | if not more | 23:15 |
kanzure | uh.. why would einstein be a measure of diversity of knowledge | 23:16 |
AshleyWaffle | you know what I mean | 23:16 |
AshleyWaffle | it's like | 23:16 |
kanzure | no i actually don't | 23:16 |
rigel | kanzure: i am quite certain that the people selling it for money are scammers, regardless of their belief in it or some scant data that suggest OMG THIS IS GREAT | 23:16 |
kanzure | selling what for money? | 23:16 |
AshleyWaffle | there's normal people, then scientists, then this sort of place | 23:16 |
kanzure | there's no lysosomal therapies on the market | 23:16 |
rigel | oh, noone's selling gray market therapies? | 23:17 |
kanzure | AshleyWaffle: http://diyhpl.us/wiki/declaration | 23:17 |
AshleyWaffle | what is lysosomal therapy | 23:17 |
kanzure | AshleyWaffle: http://diyhpl.us/~bryan/papers2/longevity/Aubrey/ | 23:17 |
rigel | oh ffs | 23:17 |
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kanzure | it's not often that people are upset that i don't type fast enough | 23:18 |
kanzure | people are getting picky these days | 23:18 |
ParahSailin | haha | 23:18 |
AshleyWaffle | hmm | 23:19 |
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jrayhawk | the more i learn about biochemistry and human health, the more grifty aubrey sounds to me | 23:58 |
--- Log closed Sun Mar 30 00:00:18 2014 |
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