| --- Log opened Thu Jun 19 00:00:03 2014 | ||
| --- Day changed Thu Jun 19 2014 | ||
| kanzure | oh, the others are columns | 00:00 |
|---|---|---|
| nmz787 | so that would be 11 | 00:00 |
| gene_hacker | well my idea of how to do it is with a bunch of syringes, and a robot that indexes between them | 00:00 |
| kanzure | nmz787: look at page 219 | 00:00 |
| kanzure | gene_hacker: you could do a rotary plate with syringes pointing down | 00:00 |
| kanzure | vertical syringes i mean | 00:00 |
| gene_hacker | we'll see | 00:01 |
| nmz787 | ParahSailin: I'll send the waste to you then | 00:01 |
| nmz787 | :D | 00:01 |
| gene_hacker | but if I make a synthesizer, I'd really like to make one of those picochip ones that uses photogenerated acid | 00:01 |
| * nmz787 looking | 00:02 | |
| gene_hacker | http://gaolab.chem.uh.edu/graphics/figure6.gif | 00:02 |
| kanzure | gene_hacker: yep, i shipped a pile of parts to nmz787 for dmd photolithography | 00:03 |
| nmz787 | but doesn't that trade-off for addressibility later? | 00:03 |
| gene_hacker | addressability? | 00:03 |
| kanzure | iirc there's a separate photochemical reaction for binding/unbinding beads that you could use | 00:03 |
| nmz787 | the point is to not have valving on-chip, right? | 00:03 |
| kanzure | yes | 00:03 |
| gene_hacker | yes, you address with light | 00:03 |
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| nmz787 | kanzure: idk about the binding/unbinding | 00:04 |
| nmz787 | i thought that would be a global operation | 00:04 |
| nmz787 | i'm familiar with this idea for making microarrays | 00:04 |
| kanzure | george church minions did micropipette pick-and-place of beads on a microarray in open air | 00:04 |
| gene_hacker | http://gaolab.chem.uh.edu/res3.htm | 00:04 |
| nmz787 | that you then remove from the column all at once and do gibson assembly on or whatever | 00:05 |
| gene_hacker | of course, one can also use a regular DNA synthesizer to drive the picoarray... | 00:05 |
| delinquentme | nmz787, https://www.facebook.com/nanoopticdevices | 00:05 |
| nmz787 | delinquentme: cool | 00:07 |
| gene_hacker | awesome, I can't wait until phones come with those built in | 00:07 |
| kanzure | you could do a microarray/picoarray without bonding to another surface (just use pressure), remove pressure/clamps when you're done, then retrieve beads with micropipette at specific locations to get specific sequences | 00:07 |
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| gene_hacker | the point of a picoarray isn't to get a specific sequence | 00:08 |
| gene_hacker | it's to generate a bunch of strands that you can combine into a big one | 00:08 |
| kanzure | if you have a 1024x768 array you might as well synthesize more than one sequence | 00:08 |
| gene_hacker | why? | 00:09 |
| nmz787 | right but then you have to do bulk-reactions, you can't do purification on each nano-reactor in the picoarray | 00:09 |
| kanzure | because if you are synthesizing the same sequence 1 million times over, you could just use a traditional column without the spatial light modulator | 00:09 |
| gene_hacker | you will still have to combine some of them | 00:09 |
| nmz787 | but avoiding bulk-reactions is much more interesting to me :P | 00:09 |
| kanzure | yes you'll always have to combine basically | 00:10 |
| gene_hacker | and if you can get the combination step to work | 00:11 |
| gene_hacker | you can make really long stuff | 00:11 |
| nmz787 | and i'm interested in doing liquid-phase chemistry, using under/near-stoichiometric ratios of reagents | 00:11 |
| gene_hacker | stuff so long it's dangerous | 00:12 |
| nmz787 | combination is fine, I just want to be sure of what I have before I add it to something else | 00:12 |
| nmz787 | I didn't mention length | 00:12 |
| nmz787 | ah, but I guess I see your point | 00:12 |
| nmz787 | delinquentme: "Now you can get a Nano-Stick spectrometer for only $2,200!" | 00:13 |
| nmz787 | delinquentme: 760nm-920nm | 00:13 |
| nmz787 | psh | 00:13 |
| nmz787 | maybe for doing fiber taps | 00:13 |
| kanzure | 760-920nm sounds inconvenient | 00:13 |
| kanzure | what about all the other spectrum | 00:13 |
| kanzure | only doing gang color approved spectrum eh? | 00:14 |
| gene_hacker | I don't think that's even gang color approved | 00:14 |
| kanzure | damn | 00:14 |
| nmz787 | paperbot: http://www.nature.com/nature/journal/v502/n7471/full/nature12607.html | 00:14 |
| kanzure | .title | 00:15 |
| yoleaux | Coherent Raman spectro-imaging with laser frequency combs | 00:15 |
| nmz787 | paperbot: http://dx.doi.org/10.1038/nature12607 | 00:15 |
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| paperbot | http://diyhpl.us/~bryan/papers2/paperbot/b56e00056b48a1fbf7d6e05b6b92d756.txt | 00:15 |
| paperbot | http://libgen.org/scimag/get.php?doi=10.1038%2Fnature12607 | 00:15 |
| nmz787 | http://diyhpl.us/~nmz787/pdf/Coherent_Raman_spectro-imaging_with_laser_frequency_combs.pdf | 00:16 |
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| nmz787 | "Resolution 0.15 nm Spectral range 500-1100 nm" | 00:17 |
| nmz787 | http://www.nanoopticdevices.com/#!products/cee5 | 00:17 |
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| -!- Topic for ##hplusroadmap: biohacking, nootropics, transhumanism, open hardware | sponsored by george church and the NRA, banned by the Federal Death Administration (4 times) | this channel is LOGGED: http://gnusha.org/logs | http://diyhpl.us/wiki | not intentionally unrepeatable | 00:24 | |
| -!- Topic set by kanzure [~kanzure@131.252.130.248] [Fri Jun 6 17:48:33 2014] | 00:24 | |
| [Users ##hplusroadmap] | 00:24 | |
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| !morgan.freenode.net [freenode-info] why register and identify? your IRC nick is how people know you. http://freenode.net/faq.shtml#nicksetup | 00:24 | |
| -!- Channel ##hplusroadmap created Thu Feb 25 23:40:30 2010 | 00:24 | |
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| kanzure | what | 00:27 |
| kanzure | oh there it is. it logged back in. | 00:28 |
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| archels | paperbot: http://iopscience.iop.org/0957-0233/25/1/015501/pdf/0957-0233_25_1_015501.pdf | 02:08 |
| paperbot | http://diyhpl.us/~bryan/papers2/paperbot/b1334cb8b50bfb771b7986074871256b.pdf | 02:08 |
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| night | Sobering approximation of the day: | 03:35 |
| night | with average luck, you get 1000 weeks of childhood, 2000 weeks of adulthood, and 1000 weeks of being old | 03:36 |
| night | that is not many weeks | 03:36 |
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| justanotheruser | Anyone here etch their own PCBs? | 06:04 |
| chris_99 | i have done a while ago | 06:09 |
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| justanotheruser | chris_99: what is the best place to buy pre-coated circuit boards | 06:24 |
| chris_99 | you mean photosensitive boards? | 06:25 |
| justanotheruser | ya | 06:25 |
| chris_99 | ah i'm in the UK, so i'd probably buy from Farnell, but Digikey or anyone should sell them | 06:26 |
| justanotheruser | ok, I'll check out digikeys selection. Gtg, thanks | 06:26 |
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| ebowden | paperbot: http://www.sciencemag.org/content/333/6047/1307 | 09:17 |
| paperbot | http://libgen.org/scimag/get.php?doi=10.1126%2Fscience.1205527 | 09:17 |
| ebowden | paperbot: http://www.sciencemag.org/content/333/6047/1307.full | 09:18 |
| paperbot | http://diyhpl.us/~bryan/papers2/paperbot/Multi-Input%20RNAi-Based%20Logic%20Circuit%20for%20Identification%20of%20Specific%20Cancer%20Cells.pdf | 09:18 |
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| nmz787_i | justanotheruser: i etched my own PCB using copper-clad, black spray paint, and a CO2 laser cutter | 09:48 |
| nmz787_i | justanotheruser: ebay has plenty of pre-coated boards though, for pretty cheap | 09:49 |
| chris_99 | is the quality the same as UV boards doing it that way though | 09:49 |
| justanotheruser | nmz787_i: any sellers you reccomend? | 09:53 |
| nmz787_i | chris_99: are you asking is the quality as good? idk never did the pre-filmed method! | 09:54 |
| chris_99 | yes i am | 09:54 |
| nmz787_i | justanotheruser: nah, cheap and decent shipping time is what i usually check | 09:54 |
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| nmz787_i | justanotheruser: as long as the reviews aren't horrible too | 09:54 |
| nmz787_i | chris_99: I was able to make a pad for an SSOP chip | 09:55 |
| justanotheruser | nmz787_i: is there a more common phrase for them? | 09:55 |
| justanotheruser | 0 results found for pre-coated circuit board | 09:55 |
| archels | search for photoresist | 10:01 |
| archels | also, consider that you can get PCBs from places like seeedstudio for *really cheap* | 10:01 |
| justanotheruser | archels: yeah, and really slow right? | 10:02 |
| chris_99 | yeah, esp. as they do > 2 layer, fancy silk screen etc. | 10:02 |
| chris_99 | not really | 10:02 |
| chris_99 | i got one from china within like a week | 10:02 |
| chris_99 | i payed extra of course though | 10:02 |
| justanotheruser | oh | 10:02 |
| justanotheruser | what is it? Like $1/per? | 10:02 |
| archels | seeed is plenty fast, if you skimp out on shipping it'll take 2 weeks longer | 10:03 |
| archels | let's see, I just paid $60 for five 10x20 cm double layer | 10:04 |
| chris_99 | i paid 64 USD for 10x 2 layer, black boards, for fast shipping | 10:04 |
| chris_99 | from Hackvana | 10:04 |
| archels | there's also oshpark.com | 10:05 |
| nmz787_i | justanotheruser: keyword should be dry-film or film pcb or photosensitive or photoresist | 10:06 |
| justanotheruser | nmz787_i: This is a good deal right? http://www.ebay.com/itm/20x-Negative-Dry-Film-Photoresist-6x8-A5-for-PCB-Prototype-DIY-Developer-Inst-/251467232236?pt=LH_DefaultDomain_0&hash=item3a8c9d73ec | 10:06 |
| archels | oshpark manufactures their boards in the US | 10:06 |
| nmz787_i | i use OSHPark... I tried seeed but I ordered from OSHPark and seeed the same night and by the time seeed was almost ready to send my boards I'd already received mine from OSHPark so I cancelled my seeed order | 10:07 |
| nmz787_i | justanotheruser: seems decent enough | 10:07 |
| justanotheruser | nmz787_i: do you usually cut your boards? | 10:07 |
| nmz787_i | I actually don't know if they get their boards from the U.S.... but they certainly break up the boards and re-mail them from the U.S. | 10:08 |
| nmz787_i | justanotheruser: I've cut a board once, a better tool is a nibbler (i think its called) | 10:08 |
| gradstudentbot | Where are the pipettes? | 10:08 |
| chris_99 | gradstudentbot, back to work boy! | 10:08 |
| gradstudentbot | We simply don't do enough titrations in my lab. | 10:08 |
| nmz787_i | justanotheruser: like this http://www.amazon.com/Parts-Express-Nickel-Plated-Nibbling/dp/B0002KRACO/ | 10:09 |
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| chris_99 | also another advantage of getting the boards made for you, they can do vias | 10:13 |
| nmz787_i | oshpark is $1.66/sq inch for 2-layer and twice that for 4-layer | 10:23 |
| kanzure | i wonder if electroosmotic flow could be used to cool a consumer-grade cpu | 10:24 |
| kanzure | why am i still using a fan to cool my cpu | 10:25 |
| chris_99 | exactly, when we could be using magnetocaloric refridgeration | 10:26 |
| kanzure | .wik magnetic refrigeration | 10:26 |
| yoleaux | "Magnetic refrigeration is a cooling technology based on the magnetocaloric effect. This technique can be used to attain extremely low temperatures, as well as the ranges used in common refrigerators." — http://en.wikipedia.org/wiki/Magnetic_refrigeration | 10:27 |
| nmz787_i | kanzure: we are looking into microfluidics in general, I don't know the details | 10:28 |
| kanzure | huh? | 10:28 |
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| nmz787_i | i doubt it would be electroosmotic though, it would probably just be heat pipes (where the heat induces a thermal current in the contained fluid) | 10:29 |
| nmz787_i | (intel) | 10:29 |
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| kanzure | justanotheruser: haha https://github.com/piggycoin/newpiggycoin/commit/c68aa2304b175d3221539c6bda138ab9d4128d2f (they ran chmod 755 on everything for no good reason) | 10:33 |
| justanotheruser | kanzure: what is the context of this? | 10:34 |
| kanzure | nvm, that is boring | 10:34 |
| justanotheruser | new scamcoin? | 10:35 |
| kanzure | oh probably | 10:35 |
| kanzure | dunno | 10:35 |
| kanzure | 10:16 < Vitallium> detronizator: I pushed latest changes to github. We should able to build PhantomJS 2 for all platforms now | 10:35 |
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| kanzure | 10:48 < sgraber_> You understand a 'good' uv formulation chemist makes +$70/hr at valspar or ppg and r&d time is normally $100/hr | 10:55 |
| kanzure | not bad | 10:55 |
| kanzure | cheap compared to programmers, lawyers, etc | 10:55 |
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| nmz787_i | kanzure: what channel was that said in? | 11:17 |
| nmz787_i | kanzure: if you could hire me a chemist... that would be insanely helpful | 11:18 |
| kanzure | shane graber is one possible chemist, but another one is david treadwell | 11:18 |
| nmz787_i | I might need to quit my day job for that | 11:18 |
| kanzure | (not the david treadwell you see on diybio) | 11:18 |
| nmz787_i | talked to local lab, it will be fine to use some sq feet of their lab bench near their fume hood | 11:19 |
| kanzure | actually i just got done sending treadwell an email a few moments ago | 11:19 |
| nmz787_i | and also other table space or cube space even for optics | 11:19 |
| kanzure | sgraber is in #dlp3dprinting: | 11:19 |
| kanzure | oops i mean #dlp3dprinting | 11:19 |
| nmz787_i | chemist or matSci | 11:20 |
| nmz787_i | or semiconductor [whatever suffix here] | 11:20 |
| kanzure | uv resin person | 11:20 |
| kanzure | oh, i mean, sgraber is uv resin person | 11:20 |
| kanzure | treadwell is anomalous | 11:20 |
| ParahSailin | what you need a chemist for | 11:22 |
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| nmz787_i | chemistry | 11:24 |
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| nmz787_i | polymer chemsitry of different sorts | 11:24 |
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| ParahSailin | trying to do novel chemistry? | 11:25 |
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| nmz787_i | sort of munging | 11:26 |
| kanzure | ParahSailin, it would be nice to have an organic synthesis person in here in general, for oligo synthesis reasons | 11:26 |
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| ParahSailin | thats actually my degree | 11:26 |
| nmz787_i | subprocess.py is importing _subprocess but I don't know where its coming from | 11:27 |
| ParahSailin | nobody ever believes me when i weigh in on stuff though | 11:27 |
| kanzure | never name your python files the same as python default modules -_- | 11:27 |
| nmz787_i | ahh | 11:27 |
| nmz787_i | is that compiled into the python.exe or some dll then? | 11:27 |
| kanzure | you're asking me if a standard python library is a standard part of python? | 11:28 |
| nmz787_i | no i'm asking can I find the source code in my Lib dir somewhere | 11:28 |
| kanzure | .py import subprocess; subprocess.__path__ | 11:28 |
| yoleaux | AttributeError: 'module' object has no attribute '__path__' | 11:28 |
| kanzure | .py import subprocess; subprocess.__file__ | 11:28 |
| kanzure | .py import subprocess; print subprocess.__file__ | 11:29 |
| yoleaux | /base/data/home/runtimes/python/python_dist/lib/python2.5/subprocess.py | 11:29 |
| nmz787_i | yeah but in subprocess.py | 11:29 |
| nmz787_i | "if mswindows: | 11:29 |
| nmz787_i | import threading | 11:29 |
| nmz787_i | import msvcrt | 11:29 |
| nmz787_i | import _subprocess" | 11:29 |
| ParahSailin | _subprocess is gonna be the c module | 11:30 |
| nmz787_i | ah http://stackoverflow.com/a/10326123/253127 | 11:30 |
| nmz787_i | what ParahSailin said | 11:30 |
| nmz787_i | subprocess.wait() seems to be crashing | 11:31 |
| nmz787_i | only the second time it's called though | 11:31 |
| nmz787_i | at least the second time in that block of code | 11:31 |
| nmz787_i | it is called elsewhere too | 11:31 |
| nmz787_i | so maybe it's the 5th time its called | 11:31 |
| nmz787_i | or 6th | 11:32 |
| dingo | what ParahSailin says, its a C module if its _modulename | 11:32 |
| dingo | i don't think you would call .wait() more than once | 11:32 |
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| dingo | should return the exit status or some such | 11:32 |
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| ParahSailin | did you strace | 11:34 |
| nmz787_i | no i didn't install cygwin | 11:34 |
| nmz787_i | someone recommends this http://technet.microsoft.com/en-us/sysinternals/bb896645.aspx | 11:36 |
| kanzure | ParahSailin: have you done organic synthesis stuff, like distillation, fractionation, glassware-fu? | 11:36 |
| kanzure | i have already spent too much of my life reading source code from sysinternals | 11:37 |
| kanzure | .title http://chem.rochester.edu/nvdcgi/commercavail.cgi | 11:38 |
| yoleaux | Should you buy it from a commercial supplier,or make it yourself? | 11:38 |
| ParahSailin | kanzure: yeah and it all sucks | 11:41 |
| kanzure | sucks more than bio? | 11:44 |
| ParahSailin | yeah because you have to do apparatus shit | 11:44 |
| ParahSailin | and weird temperatures | 11:44 |
| ParahSailin | sometimes glovebox | 11:45 |
| delinquentme | Is modifying a compound to allow it to be taken orally a "solved" process? | 12:11 |
| kanzure | yes, you put it in a capsule | 12:12 |
| delinquentme | does it just mean making it stable within a highly acidic environment | 12:12 |
| ParahSailin | enteric coated capsule | 12:13 |
| nmz787_i | delinquentme: there are enzymes and also blood brain barrier | 12:17 |
| nmz787_i | enzymes as in ones that will break it down via gut | 12:17 |
| nmz787_i | sometimes | 12:17 |
| nmz787_i | and also a molecule than gets through gut, may not be in form to make it past bbb | 12:17 |
| ParahSailin | im going to generously interpret what he was trying to ask here. can a large molecule be protected from the stomach other than by physical barrier? no not solved | 12:18 |
| nmz787_i | delinquentme: they are researching nano encapsulation stuff | 12:18 |
| kanzure | save your generosity | 12:19 |
| ParahSailin | that was one act of generosity for one github commit | 12:19 |
| delinquentme | ParahSailin, kanzure nobody has verified it even works. | 12:19 |
| * delinquentme glares | 12:19 | |
| ParahSailin | wait you didnt do a simple test of it in repl? | 12:20 |
| delinquentme | ParahSailin, thats hardly considered a "test" | 12:21 |
| delinquentme | run.dat.shit. | 12:21 |
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| delinquentme | also tldr nobody seems to have a fucking clue or care how it works | 12:21 |
| delinquentme | ask one question to kanzure have to re-ask it 10 times in different manners to get a useful answer | 12:22 |
| delinquentme | ( w regards to novel paper fetching from nature ) | 12:22 |
| kanzure | paperbot: reload papers | 12:22 |
| paperbot | kanzure: <module 'papers' from '/srv/ikiwiki/paperbot/modules/papers.py'> (version: 2013-07-09 18:45:35) | 12:22 |
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| ParahSailin | ask the right question, get the answer to the question | 12:23 |
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| ParahSailin | this how words work | 12:23 |
| kanzure | delinquentme: or maybe i'm just not advertising the method because it's in my best interest not to | 12:23 |
| kanzure | (because of obvious reasons0 | 12:23 |
| kanzure | suppose i was using a proxy installed on nih.gov | 12:24 |
| delinquentme | sure | 12:24 |
| kanzure | do you really think it's wise for me to say that in public -_- | 12:24 |
| kanzure | you guys are all terrible people | 12:24 |
| delinquentme | you've got my phone # | 12:24 |
| kanzure | anyway, the source code is totally accurate and nothing is missing on github | 12:24 |
| delinquentme | and im sure you have more than enough info to blackmail me | 12:24 |
| kanzure | i would call you, except there is literally no missing component that you haven't seen | 12:24 |
| delinquentme | I'm just conveying that there is a small even if loose circle of trust | 12:25 |
| kanzure | erm, i mean, literally no component that you haven't seen | 12:25 |
| kanzure | no magic | 12:25 |
| delinquentme | at the time it seemed like it was | 12:25 |
| kanzure | paperbot: reload papers | 12:25 |
| paperbot | kanzure: papers: no such module! | 12:25 |
| ParahSailin | im also virtually certain that the specifics have at one time or another been publicly discussed in this channel | 12:26 |
| kanzure | huh where did the module go | 12:26 |
| kanzure | whybreak? | 12:26 |
| kanzure | Error loading scihub: [Errno 2] No such file or directory: 'libgen_login.txt' (in bot.py) | 12:26 |
| kanzure | whodidthis | 12:26 |
| delinquentme | ParahSailin, so I saw this lovely little paper http://www.nature.com/nchembio/journal/vaop/ncurrent/full/nchembio.1551.html and it seems like it would be something of HUGE interest to both wetlab workers that I've got over here + generics manufacturers | 12:27 |
| delinquentme | but currently at the " someone pay me for it " stage | 12:27 |
| delinquentme | need pharma insiders | 12:28 |
| ParahSailin | http://libgen.org/scimag/get.php?doi=10.1038%2Fnchembio.1551 | 12:28 |
| kanzure | why are we using a text file | 12:28 |
| kanzure | git blame says it's delinquentme's fault | 12:28 |
| delinquentme | thats what I decided to go w | 12:29 |
| ParahSailin | you guys had a thing where you decided the secret genesis/upload lp doesnt belong in the repo | 12:29 |
| ParahSailin | even though it is in plaintext on a russian hacker site | 12:29 |
| kanzure | https://github.com/kanzure/paperbot/pull/29 | 12:29 |
| kanzure | i must have missed that part of the conversation | 12:30 |
| delinquentme | it was in heeere | 12:31 |
| delinquentme | emailed the creds file kanzure | 12:33 |
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| gnusha_ | https://secure.diyhpl.us/cgit/paperbot/commit/?id=c9bd6e7a Bryan Bishop: back to hardcoding a password? | 12:39 |
| gnusha_ | paperbot: reload papers | 12:39 |
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| delinquentme | paperbot, worlddomination() | 12:41 |
| delinquentme | ParahSailin, 3 commits. I want a hug. | 12:42 |
| delinquentme | and a promise ring. | 12:42 |
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| kanzure | https://github.com/dcowden/cadquery | 13:06 |
| kanzure | huh, so it turns out that freecad's python modules can be used without running the freecad executable | 13:07 |
| kanzure | and without using freecad's embedded python interpreter | 13:07 |
| chris_99 | i was using FreeCad recently thought it was pretty good, except when it crashed | 13:08 |
| chris_99 | (that could be because i was using the Windows version?) | 13:08 |
| kanzure | no, it crashes everywhere | 13:10 |
| kanzure | opencascade is crashy | 13:11 |
| kanzure | that is why freecad crashes | 13:11 |
| chris_99 | ah | 13:11 |
| delinquentme | le brb | 13:16 |
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| kanzure | .wik PLaSM | 13:37 |
| yoleaux | "PLaSM (Programming Language of Solid Modeling) is an open source scripting language for solid modeling, a discipline that constitutes the foundation of computer-aided design and CAD systems. In contrast to other CAD programs, PLaSM emphasizes scripting rather than interactive GUI work." — http://en.wikipedia.org/wiki/PLaSM | 13:37 |
| kanzure | http://www.plasm.net/ | 13:37 |
| kanzure | "1. Plasm primitives are ALL CAPS (all capital letters)" ugh | 13:38 |
| chris_99 | weird | 13:39 |
| kanzure | https://github.com/cvdlab/plasm-crumbs-slides/blob/master/chapters/surfaces/Readme.md | 13:40 |
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| kanzure | # example of boolean intersection | 13:49 |
| kanzure | Plasm.view(Plasm.boolop(BOOL_CODE_AND,[Plasm.cube(2,-0.5,0.5),Plasm.rotate(Plasm.cube(2,-0.5,0.5),2,1,2,pi/4)])) | 13:49 |
| kanzure | probably refers to https://github.com/plasm-language/pyplasm/blob/master/src/xge/plasm.boolop.cpp | 13:49 |
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| kanzure | or this one: https://github.com/femhub/plasm/blob/master/src/xge/plasm.boolop.cpp | 13:51 |
| kanzure | looks like a polyhedral surface intersection meto | 13:52 |
| kanzure | *method | 13:52 |
| kanzure | oh well | 13:52 |
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| kanzure | paperbot: http://www.ingentaconnect.com/content/asp/jctn/2008/00000005/00000005/art00002 | 13:59 |
| kanzure | .title | 13:59 |
| kanzure | i don't remember seeing a 100 page paper about this | 14:00 |
| yoleaux | A Minimal Toolset for Positional Diamond Mechanosynthesis: ingentaconnect | 14:00 |
| paperbot | http://diyhpl.us/~bryan/papers2/paperbot/52b613cfcb8fb9744bf22452ed2828a1.txt | 14:00 |
| kanzure | oh it was probably this one http://www.molecularassembler.com/Papers/MinToolset.pdf | 14:00 |
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| nmz787_i | kanzure: for a second i thought that url was molecularembarraser.com | 16:34 |
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| kanzure | http://gizmodo.com/5013018/boeing-successfully-fires-25-kw-solid-state-lasers-laser-weapons-one-step-closer-to-being-a-reality | 16:42 |
| gradstudentbot | I already have one of those in my collection, you know. | 16:42 |
| kanzure | 50 W laser diode for $500 http://www.alibaba.com/product-detail/high-performance-diode-laser-808nm-50W_1219796601.html | 16:44 |
| chris_99 | that seems really cheap | 16:45 |
| chris_99 | are they more or less reliable than CO2? | 16:45 |
| kanzure | no clue | 16:46 |
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| streety | something doesn't add up there ... max power 50W, max forward current 1.2A, max forward voltage 2.2V | 17:36 |
| streety | only two of those can be correct | 17:36 |
| chris_99 | haha yeah | 17:36 |
| ThomasEgi | streety, lasers? | 17:38 |
| gene_hacker | 50 watts pulsed probably | 17:38 |
| gene_hacker | yup 808 nm, that's probably a range finding laser | 17:38 |
| ThomasEgi | 808 doesn't sound like CO2 tho | 17:39 |
| chris_99 | it's a diode | 17:39 |
| ThomasEgi | diode laser? | 17:39 |
| gene_hacker | yes | 17:39 |
| chris_99 | yeah scroll up | 17:39 |
| gene_hacker | http://www.repairfaq.org/sam/laserdio.htm#diocss7b | 17:40 |
| chris_99 | ah | 17:41 |
| chris_99 | interesting | 17:41 |
| chris_99 | can you get non-pulsed high power laser diodes | 17:42 |
| ThomasEgi | those are usualy arrays tho. | 17:43 |
| kanzure | that's unfortunate | 17:43 |
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| chris_99 | http://www.ebay.com/itm/50W-CW-Diode-Pumped-Nd-YAG-Cavity-DPSS-Laser-Module-/270605121972?pt=LH_DefaultDomain_0&hash=item3f015279b4 | 17:51 |
| gene_hacker | now if we could only manage to make an array of phase locked laser diodes... | 17:56 |
| kanzure | "Buyers must be 18 years old" | 17:57 |
| kanzure | that is an oddly specific target market.. | 17:58 |
| kanzure | 18 year olds that want 50 W solid state lasers | 17:58 |
| chris_99 | haha | 17:58 |
| ThomasEgi | they won't phase lock | 17:59 |
| ThomasEgi | but you could use them to pump another laser | 17:59 |
| ThomasEgi | wait. that IS a diode pumped laser | 18:00 |
| gradstudentbot | If you help me, I'll put you on the paper. | 18:00 |
| ThomasEgi | those numbers don't make sensetho. | 18:01 |
| chris_99 | what numbers? | 18:02 |
| ThomasEgi | current, voltage, power | 18:02 |
| ThomasEgi | iirc nd-yag have typical efficiencies around 1% or so. | 18:02 |
| nmz787_i | on the ebay page? | 18:02 |
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| ThomasEgi | yup | 18:03 |
| nmz787_i | ThomasEgi: it might make sense if they're including the non-central output freqs | 18:03 |
| chris_99 | http://www.sino-laser.com/pdf/GN50_spec.pdf | 18:04 |
| chris_99 | not much more info | 18:05 |
| nmz787_i | has some higher numbers | 18:06 |
| ThomasEgi | well it's not entirely off. | 18:06 |
| ThomasEgi | given it's diode laser pumped its efficiency might be a bit higher than traditional flash tube pumping | 18:07 |
| ThomasEgi | now.. time for sleep. exams tomorrow.. coincidently.. an exam about lasers | 18:07 |
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| gene_hacker | they won't phase lock yet | 18:14 |
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| kanzure | paperbot: http://pubs.acs.org/doi/abs/10.1021/ed083p919 | 21:13 |
| kanzure | .title | 21:13 |
| yoleaux | Student Empowerment through 'Mini-microscale' Reactions: The Epoxidation of 1 mg of Geraniol | 21:13 |
| paperbot | http://libgen.org/scimag/get.php?doi=10.1021%2Fed083p919 | 21:13 |
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| ||0_-_0|| | paperbot http://www.nature.com/nbt/journal/v25/n11/abs/nbt1356.html | 21:19 |
| kanzure | http://libgen.org/scimag/get.php?doi=10.1038%2Fnbt1356 | 21:19 |
| paperbot | http://libgen.org/scimag/get.php?doi=10.1038%2Fnbt1356 | 21:20 |
| ||0_-_0|| | paperbot http://link.springer.com/article/10.1007/BF02462863 | 21:34 |
| paperbot | http://diyhpl.us/~bryan/papers2/paperbot/588841dac2dc9487050294e016b6b61a.txt | 21:34 |
| nmz787_i | i wonder if there are places around here that do etching as a service | 21:47 |
| nmz787_i | if the microscope + blu-ray works to make a photoresist mask... then I'd think it would work for etching protection with some process or another | 21:48 |
| nmz787_i | in silicon | 21:48 |
| nmz787_i | or glass | 21:48 |
| gradstudentbot | Should I be doing this in the fume hood? | 21:49 |
| kanzure | naaah it'll be fine, radstudentbot | 21:54 |
| gene_hacker | I think you should listen to it, it has a valid point | 22:00 |
| kanzure | fumes build character | 22:00 |
| gene_hacker | at least use a fan or something, like those diy fume extractors | 22:00 |
| gene_hacker | and did you ever figure out how that group in diybio is going to build a synth? | 22:02 |
| gene_hacker | what are they using magnetic beads for? | 22:02 |
| kanzure | haven't received any information from them | 22:02 |
| ||0_-_0|| | worth a try | 22:02 |
| ||0_-_0|| | paperbot http://link.springer.com/article/10.1007%2Fs10516-007-9011-2?LI=true | 22:02 |
| paperbot | http://diyhpl.us/~bryan/papers2/paperbot/2048d088a483941d4398cbfbe226d883.txt | 22:02 |
| gene_hacker | I wonder if they'll be able to do a kilobase, because if they do, things could get interesting... | 22:03 |
| kanzure | we could do the picoarray setup | 22:04 |
| kanzure | haven't found a vendor for the photogenerated acid inputs | 22:05 |
| kanzure | (haven't looked, though) | 22:05 |
| gene_hacker | I think it's shouldn't be too hard to find | 22:06 |
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| kanzure | .wik luer-lock joint | 22:06 |
| yoleaux | "A syringe is a simple pump consisting of a plunger that fits tightly in a tube. The plunger can be pulled and pushed along inside a cylindrical tube (called a barrel), allowing the syringe to take in and expel a liquid or gas through an orifice at the open end of the tube. The open end of the syringe may be fitted with a hypodermic needle, …" — http://en.wikipedia.org/wiki/Syringe | 22:06 |
| kanzure | what just happened | 22:06 |
| gene_hacker | anyway, I'm wondering some stuff about diamonoid mechanosynthesis | 22:07 |
| gene_hacker | just how stiff a manipulator one needs to do it | 22:07 |
| kanzure | well, you need to survive jamming it into surfaces randomly (crashing the tip) | 22:10 |
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| gene_hacker | easy enough | 22:10 |
| kanzure | have you read the tooltip paper? it might be in there | 22:13 |
| kanzure | many things might be in there.. | 22:13 |
| gene_hacker | I'll have a look | 22:13 |
| kanzure | in http://www.molecularassembler.com/Papers/MinToolset.pdf | 22:13 |
| kanzure | page 764 mentions compressive loads and stiffness.. | 22:14 |
| gene_hacker | excellent | 22:16 |
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| gene_hacker | I think that's just the tool tip | 22:17 |
| kanzure | pffft so demanding :) | 22:18 |
| gene_hacker | I'll find it somewhere | 22:19 |
| kanzure | .wik luer taper | 22:19 |
| yoleaux | "The Luer taper is a standardized system of small-scale fluid fittings used for making leak-free connections between a male-taper fitting and its mating female part on medical and laboratory instruments, including hypodermic syringe tips and needles or stopcocks and needles." — http://en.wikipedia.org/wiki/Luer_taper | 22:19 |
| kanzure | paperbot: http://www.iso.org/iso/iso_catalogue/catalogue_tc/catalogue_detail.htm?csnumber=4693 | 22:20 |
| paperbot | http://diyhpl.us/~bryan/papers2/paperbot/5426d8a03004c94623bf35f7b939f1bc.txt | 22:21 |
| kanzure | damn | 22:21 |
| kanzure | the photogenerated acid paper used "biaryl iodium PGA-P (Secant Chemicals) (3% in CH2Cl2) and photosensitizing thioxanthenone (Sigma-Aldrich). The solution was protected from light during preparation and storage." | 22:25 |
| kanzure | "In a PGA deprotection step, droplet formation was achieved by filling the reactor from bottom to top with PGA-P solution, and then draining the solution from the bottom of the reactor. The flow direction was controlled by a four-way electronic valve (Valco)." | 22:26 |
| kanzure | "The light-directed acid deprotection steps lasted 0.5 - 2 min and were followed by a rapid pyridine/CH3CN (10/90 v/v) wash. The rest of the synthesis steps were coupling, capping and oxidation and they were the same as performed in conventional oligonucleotide synthesis." | 22:26 |
| kanzure | "coupling reaction using a mixture 1:10 of fluorescein:T phosphoramidites" sounds nice | 22:27 |
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| kanzure | hm, their microarray had wells with a diameter of 150-600 microns. that's pretty big. | 22:30 |
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| gene_hacker | well within the range of what can be done with a projector | 22:36 |
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| gene_hacker | the hard part is optimizing the chambers for equal flow | 22:39 |
| kanzure | i'm not sure it needs to have chambers | 22:39 |
| gene_hacker | but that shouldn't be too hard with | 22:39 |
| gene_hacker | true | 22:39 |
| kanzure | what were the chambers for? | 22:39 |
| gene_hacker | isolating the photogenerated acid | 22:40 |
| gene_hacker | preventing it from uncapping that which you don't want uncapped | 22:41 |
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| kanzure | wouldn't that happen during the wash cycle? the acids would get pushed around to the other chambers. | 22:51 |
| gene_hacker | I don't know | 22:51 |
| gene_hacker | now where was that paper again? | 22:52 |
| kanzure | which one would you like | 22:53 |
| gene_hacker | http://www.lcsciences.com/wp-content/uploads/picoarray.png | 22:53 |
| gradstudentbot | We were out of the right dye, so I just used an equivalent. | 22:53 |
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| gene_hacker | nope it's simpler than that | 22:53 |
| kanzure | http://diyhpl.us/~bryan/papers2/microfluidics/synthesis/Synthesis%20-%20Microfluidic%20PicoArray%20synthesis%20of%20oligodeoxynucleotides%20and%20simultaneous%20assembling%20of%20multiple%20DNA%20sequences%20(10%20kb).pdf | 22:53 |
| gene_hacker | there are dedicated waste channels | 22:53 |
| gene_hacker | take a look a the arrows on the picture | 22:53 |
| kanzure | looks like all the same channel to me | 22:54 |
| gene_hacker | look closer | 22:54 |
| gene_hacker | the acid gets washed out into the waste channels | 22:55 |
| kanzure | osnap you're right | 22:55 |
| gene_hacker | not into other chambers | 22:55 |
| kanzure | those waste channels are not connected to the main inlet | 22:55 |
| kanzure | huh | 22:55 |
| gene_hacker | correct | 22:55 |
| gene_hacker | they are connected to the outlet | 22:55 |
| kanzure | yep i totally missed that | 22:55 |
| gradstudentbot | It's not really significant, but there's definitely a trend. | 22:56 |
| gene_hacker | and that's why you have to carefully optimize the channels | 22:57 |
| gene_hacker | of course it's low Re flow, it can't be that bad | 22:57 |
| kanzure | waste channel could be deeper, maybe | 22:57 |
| gene_hacker | or 3d | 22:58 |
| gene_hacker | however, aren't the chemicals needed nasty solvents? | 22:58 |
| kanzure | nmz787 has been looking into material compatibility things | 23:01 |
| gene_hacker | now I was going to say ceramic, but ceramic isn't clear | 23:02 |
| gene_hacker | no wait, it can be | 23:02 |
| gene_hacker | it's just insane: http://en.wikipedia.org/wiki/Transparent_ceramics | 23:03 |
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| --- Log closed Fri Jun 20 00:00:40 2014 | ||
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