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archels | http://matplotlib.org/users/whats_new.html#xkcd-style-sketch-plotting | 05:57 |
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archels | tomfoolery | 05:57 |
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kanzure | .title https://www.youtube.com/watch?v=eXXmUWD9_0w | 06:16 |
yoleaux | Mt Lofty Television Transmitter Station: A Brief Tour | 06:16 |
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chris_99 | ahhh paperbot where art thou | 06:54 |
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eudoxia | i like to imagine a big bot in a hospital bed with a sad emoji on a screen | 06:56 |
eudoxia | 🙀 | 06:57 |
eudoxia | that's U+1F640 btw | 06:57 |
chris_99 | hehe | 06:57 |
chris_99 | poor paperbot | 06:58 |
kanzure | http://img.photobucket.com/albums/v314/streamwolf8/Anime%20North%202008/DSCF0860.jpg | 06:58 |
kanzure | http://fc06.deviantart.net/fs71/f/2013/148/1/0/flcl__canti_by_deckronomicon-d66zl5u.jpg | 06:59 |
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kanzure | ParahSailin: what was your comment about antivenom about? | 08:18 |
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ParahSailin | i was surprised to find out that antivenom is typically just polyclonal antibody extracted as serum | 08:19 |
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kanzure | i think in cases of scorpion antivenom they are literally just raising scorpions on farms and extracting fluids regularly | 08:21 |
kanzure | wasn't there something about an antivenom shortage or something | 08:21 |
kanzure | "Why is there shortage of coral snake antivenom? Pfizer/Wyeth has been the sole producer of antivenom in the United States. They no longer are manufacturing it. When present stocks are gone there, will be no FDA-approved antidote for coral snake bites." | 08:22 |
ParahSailin | there was, in australia or something a few years ago | 08:22 |
kanzure | oh brother "Before antivenin was developed in 1967, the fatality rate was reported as high as 10-20%." | 08:23 |
kanzure | "What’s being done about the antivenom shortage?" "Managing and distributing the remaining Antivenin (Micrurus fulvius)®" "Wyeth restarted the manufacturing process, and is planning to reintroduce Antivenin (Micrurus Fulvius) ®. It is unknown how soon a new supply will be available." | 08:23 |
cluckj | 1967? | 08:23 |
ParahSailin | kanzure: yeah, if you have polyclonal serum production system, you need regular supply of mammals and antigen | 08:24 |
cluckj | that's a little bit late iirc | 08:24 |
kanzure | "Each vial of CroFab costs a hospital about $2,000" haha | 08:24 |
cluckj | maybe poisonous snakes could just be eradicated | 08:25 |
cluckj | WAR ON VENOM | 08:25 |
cluckj | SHOCK AND AWE | 08:25 |
kanzure | or you can just introduce the antibody in advance | 08:25 |
cluckj | http://clinicaltrials.gov/ct2/show/NCT02081326 | 08:29 |
kanzure | .title | 08:29 |
yoleaux | Repeat BCG Vaccinations for the Treatment of Established Type 1 Diabetes | 08:29 |
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cluckj | I think that's my FDA hate for the next few weeks | 08:30 |
cluckj | study completion: 2022 | 08:31 |
kanzure | "Antivenin is the proper term for animal derived serums which are more like vaccines than traditional chemically manufactured medicines. Antivenom is the general term for anything else derived through non-biological substances." | 08:31 |
kanzure | hmmm | 08:31 |
cluckj | meanwhile billions of people get that safe and effective vaccine | 08:32 |
cluckj | maybe I can convince my doctor to give it to me | 08:32 |
kanzure | do it yourself | 08:32 |
cluckj | yeah | 08:33 |
cluckj | the county department of health probably has that vaccine | 08:33 |
kanzure | antivenin/antivenom is a highly unfortunate distinction | 08:35 |
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heath | another reason not to use the ybit nick | 10:36 |
heath | 10:35 < sinious> >> var areYouMyBitch = true; console.log(areYouMyBitch + '!'); | 10:36 |
heath | ^ that highlights | 10:36 |
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heath | find . | 11:11 |
heath | didn't realize that acted like tree | 11:11 |
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eudoxia | i came up with a new line for gradstudentbot | 11:17 |
eudoxia | "The electron microscope burned my sample" | 11:17 |
eudoxia | ahahahahahahhahha | 11:18 |
kanzure | did this happen to you? | 11:19 |
eudoxia | no | 11:21 |
eudoxia | does that mean it's disqualified? | 11:21 |
kanzure | no | 11:21 |
eudoxia | anyways i read in Richard Jones' heretical anti-MNT book that it's a thing that happens | 11:25 |
eudoxia | his PhD was originally in how globular proteins move in fluids and the TEM kept burning his samples :V | 11:26 |
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||0_-_0|| | just show me the pictures | 11:27 |
||0_-_0|| | paperbot http://www.sciencemag.org/content/344/6190/1373 | 11:27 |
paperbot | http://libgen.org/scimag/get.php?doi=10.1126%2Fscience.1252291 | 11:27 |
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dingo | i wrote my own chat server, telnet 1984.ws 6023 | 12:25 |
dingo | its an all-in-one irssi-like clone | 12:26 |
dingo | hehe | 12:26 |
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kanzure | The black thing inside rejoices at your departure. | 12:30 |
dingo | :-) | 12:30 |
dingo | i got that from an old dos game, "legend of the reg dragon" | 12:31 |
kanzure | ParahSailin: would you be happy with just doing the 1918 influenza virus, or does it have to be a modern supervirus? | 12:31 |
ParahSailin | do what? | 12:31 |
kanzure | for your freedom virus | 12:32 |
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ParahSailin | not any extra trouble to do modern supervirus i suppose | 12:34 |
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kanzure | 100k bp per $1 | 12:46 |
kanzure | oh.. the inkjet dna synthesizer that church is referring to is actually posam/pogo http://bioinformatics.org/pogo/ | 12:47 |
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kanzure | i wish they would just say that. it was burried 5 references deep. | 12:47 |
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kanzure | gl45 cap connection system http://www.duran-group.com/uploads/pics/uebersicht_ASS-GL45sm_05.jpg | 13:23 |
kanzure | they spent $23,000 on their motion control and actuation stuff? http://bioinformatics.org/pogo/POSAM_Man_Ch1_Assembly_v1-2_040601.pdf | 13:26 |
ParahSailin | the inkjet microarray printing is pretty much obsoleted by photolithography microarray printing | 13:28 |
kanzure | but with inkjetting you don't need photolabile protecting groups or photogenerated acids | 13:42 |
kanzure | and the droplets are separate microreactors | 13:42 |
ParahSailin | i will have to do literature review to figure out why photolithography made the inkjet method obsolete | 13:43 |
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ParahSailin | hm, it might be just as simple as photolithography allowed higher spot density, which is not much of a plus in this case | 14:01 |
ParahSailin | there is nothing on posam after 2004? | 14:06 |
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kanzure | ParahSailin: these guys used it for a thing published 2010, http://dukespace.lib.duke.edu/dspace/bitstream/handle/10161/4000/274747200023.pdf%3Fsequence%3D1 | 14:45 |
ParahSailin | i recognize some common names with the quan paper | 14:46 |
kanzure | same group that referenced it, this time 2011 inkjet | 14:46 |
kanzure | oops | 14:46 |
kanzure | this time 2011 http://diyhpl.us/~bryan/papers2/DNA/Parallel%20on-chip%20gene%20synthesis%20and%20application%20to%20optimization%20of%20protein%20expression.pdf | 14:46 |
ParahSailin | yeah the quan paper | 14:46 |
kanzure | double oops, yes | 14:47 |
kanzure | photolithography is a plus if you're already doing photolithography anyway | 14:48 |
kanzure | another reason might be that everyone was butthurt by affymetrix's weirdo photomask-based photolithography system | 14:48 |
kanzure | where 25 bp sequences required 100 masks | 14:49 |
kanzure | erm, i don't know which direction that reason was supposed to be suporting | 14:49 |
kanzure | *supporting | 14:50 |
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kanzure | one of the gen9bio people does art for http://collisioncollective.org/content/about-collisioncollective ("a group show dealing with two closely-related concepts: human self-modification and the human modification of our environment") | 15:15 |
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eudoxia | kanzure: the other day you said other h+ communities made us look like demigods | 15:17 |
kanzure | definitely | 15:18 |
eudoxia | what would be the ten Herculean Labors? | 15:18 |
eudoxia | of transhumanism | 15:18 |
kanzure | i don't know, you figure it out | 15:18 |
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kanzure | the point is, writing magazine articles doesn't magically make LSD | 15:19 |
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||0_-_0|| | kanzure Demigod of Grumpywit | 15:26 |
kanzure | i said *seem* like demigods, | 15:26 |
kanzure | any advanced.. sufficiently magic.. etc. | 15:26 |
||0_-_0|| | Grumpywit and Literalism | 15:27 |
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dbolser | I think I'm h- | 15:49 |
gnusha_ | https://secure.diyhpl.us/cgit/diyhpluswiki/commit/?id=f88d4b09 Bryan Bishop: elaborate opencascade methods >> http://diyhpl.us/diyhpluswiki/cad/opencascade/ | 15:57 |
gnusha_ | https://secure.diyhpl.us/cgit/diyhpluswiki/commit/?id=4382edcd Bryan Bishop: some extruded aluminum scaffolding >> http://diyhpl.us/diyhpluswiki/scaffolding/ | 15:57 |
kanzure | "We use uv-cureable polymers and printed masks for the mold, PDMS & SIFEL (fluorinated silicon) for the chip, vakuum pumps to degas the PDMS and corona treatment to bond the PDMS to glass." | 16:04 |
gnusha_ | https://secure.diyhpl.us/cgit/diyhpluswiki/commit/?id=6d6a42c7 Bryan Bishop: gah, i forgot about gridbeam >> http://diyhpl.us/diyhpluswiki/scaffolding/ | 16:06 |
gnusha_ | https://secure.diyhpl.us/cgit/diyhpluswiki/commit/?id=f480398b Bryan Bishop: ah, defer to reprap for scaffolding >> http://diyhpl.us/diyhpluswiki/scaffolding/ | 16:10 |
kanzure | "Parametrically Designed XY Motion Stage" http://web.mit.edu/imoyer/www/portfolio/parametric/ | 16:14 |
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kanzure | .wik asco numatics | 18:01 |
yoleaux | "The Emerson Electric Company (NYSE: EMR) is an American multinational corporation headquartered in Ferguson, Missouri, United States. This Fortune 500 company manufactures products and provides engineering services for a wide range of industrial, commercial, and consumer markets." — http://en.wikipedia.org/wiki/ASCO_Numatics | 18:01 |
kanzure | oh hm. | 18:02 |
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kanzure | cadquery is pretty fun when used inside freecad | 20:55 |
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ebowden | Imagine scritching the ears when they take they hat off after a long day. | 21:15 |
ebowden | Ooh, wrong window. | 21:16 |
ebowden | :D | 21:16 |
kanzure | very wrong | 21:18 |
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kanzure | i think i'll convert mcad.git into cadquery | 21:46 |
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gene_hacker | PDMS swells with most solvents used in DNA synthesis | 22:48 |
gene_hacker | but with inkjetting, you need a special inkjet that can handle weird stuff | 22:49 |
nmz787_i | yup | 22:50 |
nmz787_i | or fluridated polymers | 22:50 |
nmz787_i | fluoridated* | 22:50 |
gene_hacker | and those special inkjets could be really cheap too if it wasn't for that darned HP | 22:51 |
gene_hacker | or other inkjet manufacturers who aren't willing to sell inkjet devkits for fear of people making their own replacement inks | 22:53 |
gene_hacker | those kilobaser people do have an interesting method of doing corona treatment though | 22:55 |
gene_hacker | they use a microwave oven | 22:55 |
nmz787_i | that's how they usually work | 22:55 |
nmz787_i | the lab-branded ones just have tunable freq | 22:55 |
nmz787_i | also there's a video on youtube from a few years ago showing the whole setup | 22:56 |
nmz787_i | those guys never emailed me back anyway | 22:56 |
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gene_hacker | well if they can get 1 kilobase with low enough errors, things could get interesting | 22:58 |
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--- Log closed Tue Jun 24 00:00:44 2014 |
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