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Adlai | maaku: it, it, ... them? | 05:07 |
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Adlai | :D | 05:07 |
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chris_99 | Does anyone know of any studies comparing a stir bar vs an orbital shaker for things like growing yeast | 07:09 |
yoleaux | 11 Apr 2015 00:57Z <nmz787> chris_99: ah, cool! what are you gonna use to control the CCD? | 07:09 |
chris_99 | or have any options | 07:09 |
chris_99 | !help | 07:10 |
chris_99 | hmm, how do i leave a message with yoleaux too heh | 07:10 |
cluckj | I haven't seen stir bars used for growing yeast on a small scale | 07:11 |
chris_99 | oh, they're used a lot in the homebrewing world, but im wondering if a shaker would be the same in performance | 07:12 |
cluckj | stir bars tend to be attached to hot plates, and orbital shakers tend to also be incubators | 07:12 |
cluckj | oh | 07:12 |
chris_99 | i've found an orbital shaker with a hotplate | 07:12 |
chris_99 | but i'm not sure if i should get a stir plate intead | 07:14 |
cluckj | why not both? | 07:14 |
chris_99 | heh, i don't have much spare cash atm | 07:15 |
cluckj | oh hah | 07:15 |
cluckj | I think it might be a scale issue....an orbital shaker that can do a 15 gallon drum of beer is gonna be scary as fuck | 07:16 |
chris_99 | oh to make it clear, this is just for a yeast starter | 07:18 |
chris_99 | so only say 1litre or 2litre | 07:18 |
chris_99 | although a 15 gal one would be cooler :) | 07:21 |
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cluckj | haha | 07:28 |
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Adlai | paperbot: http://pubs.rsc.org/en/Content/ArticleLanding/2013/CS/c3cs35433d | 08:05 |
paperbot | http://diyhpl.us/~bryan/papers2/paperbot/New%20insights%20into%20prebiotic%20chemistry%20from%20Stanley%20Miller%27s%20spark%20discharge%20experiments.pdf | 08:06 |
Adlai | esgeht! | 08:07 |
Adlai | paperbot: http://onlinelibrary.wiley.com/doi/10.1002/anie.198308161/pdf | 08:14 |
paperbot | http://diyhpl.us/~bryan/papers2/paperbot/825c1ef0c098892697e4bd594410ed76.txt | 08:14 |
kanzure | hmph | 08:14 |
Adlai | hmm. | 08:14 |
* Adlai should get to "work" anyway | 08:15 | |
kanzure | bah | 08:15 |
kanzure | or file bug reports on paperbot | 08:15 |
kanzure | https://github.com/kanzure/paperbot | 08:15 |
Adlai | ah, but https://github.com/kanzure/paperbot/issues/22 already exists :P | 08:21 |
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catern | https://mchankins.wordpress.com/2013/04/21/still-not-significant-2/ | 08:30 |
catern | lol | 08:30 |
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Adlai | "For example, “a trend towards significance” expresses non-significance as some sort of motion towards significance, which it isn’t: there is no ‘trend’, in any direction, and nowhere for the trend to be ‘towards’" | 08:32 |
CheckDavid | What do you guys think of fasting? | 08:37 |
Adlai | leeches have also been said to cure numerous ailments | 08:38 |
kanzure | CheckDavid: also consider tapeworms | 08:40 |
CheckDavid | You drawing an analogy between leeches and fasting Adlai ? | 08:41 |
Adlai | yes. are you thinking of fasting? the answer to "why or why not" either makes my analogy relevant or irrelevant... fasting "done right" probably has more psychological benefit than leeches | 08:43 |
FourFire | CheckDavid, Honey as disinfectant | 08:43 |
CheckDavid | I did already a 2 day fasting | 08:43 |
CheckDavid | Oh heard that one before FourFire | 08:43 |
FourFire | Do you understand the mechanism behind it? | 08:43 |
CheckDavid | FourFire: fasting? | 08:44 |
CheckDavid | No I am not aware of the mechanics. | 08:44 |
kanzure | whoops i mean tapeworm eggs | 08:44 |
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CheckDavid | Do you FourFire ? | 08:45 |
kanzure | i don't even know if non-egged tapeworms work | 08:45 |
CheckDavid | They work very hard man. | 08:45 |
FourFire | CheckDavid, no honey | 08:47 |
CheckDavid | I read some shit online about fasting and was very intrigued | 08:47 |
cluckj | there is some dude whose lab I wanted to get into who was grinding up whipworms and giving them to people | 08:47 |
cluckj | to cure a whole bunch of autoimmune conditions | 08:47 |
CheckDavid | People reporting feeling energetic after 9 days of nutrient deprivation and sucb | 08:47 |
CheckDavid | I had to try it | 08:47 |
CheckDavid | That's how intrigued I was | 08:48 |
CheckDavid | *such | 08:48 |
CheckDavid | But only for like 60 hours | 08:48 |
CheckDavid | Lil over 48 hours | 08:48 |
CheckDavid | Barely a proper experience | 08:48 |
cluckj | starvation conditions change your biochemistry | 08:48 |
CheckDavid | But still interesting | 08:49 |
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CheckDavid | cluckj: can you elaborate? | 08:49 |
cluckj | CheckDavid, your body basically empties out its local energy storage, then starts consuming functional proteins, then your fat cells dump all their storage | 08:50 |
Adlai | when an animal starves, it ceases storing fat, and begins digesting stored fat | 08:50 |
cluckj | there are some diets that can forcce your body into that kind of mode tho | 08:50 |
Adlai | iiuc you don't start consuming functional proteins until you're in quite a dilipidated state | 08:50 |
cluckj | keto diets do that, where you put yourself into a state of ketogenesis | 08:50 |
cluckj | Adlai, I thought so too but iirc your body does the intercellular stuff first | 08:51 |
Adlai | fasting also makes you metabolize more rapidly once you ingest, so it's also used to accelerate/intensify psychoactive effects | 08:53 |
cluckj | yeah | 08:53 |
cluckj | when you do the keto diet, if you eat too many carbs you ruin the ketogenic state | 08:54 |
CheckDavid | Some say muscle goes first | 08:54 |
CheckDavid | Others say fat goes first | 08:54 |
CheckDavid | Which is more accurate? | 08:54 |
cluckj | I think muscle does | 08:55 |
cluckj | in starvation conditions | 08:55 |
cluckj | it's stupid but the human body was not intelligently designed :) | 08:55 |
cluckj | and it's a temporary state | 08:55 |
cluckj | it's not like you run out of muscles before your body starts eating fat stores | 08:56 |
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kanzure | there should be a muscle eating starvation curve chart | 08:56 |
cluckj | there probably is | 08:56 |
cluckj | from my own personal experience, muscle is definitely higher on the consuming/producing curve than fat | 08:58 |
cluckj | I've been trying to gain some fat back from the undiagnosed diabetes starvation conditions for like 2 years, and all I can get is useless shitty muscle | 09:01 |
cluckj | (I need fatty places to stick cannulas and sensors) | 09:01 |
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CheckDavid | damn that's crazy cluckj | 10:03 |
CheckDavid | Some people will be envious | 10:03 |
CheckDavid | Like me | 10:04 |
CheckDavid | I'm envious that you get so much useless muscle | 10:04 |
CheckDavid | And no fat | 10:04 |
cluckj | lol | 10:04 |
cluckj | it was because of undiagnosed type 1 diabetes | 10:05 |
cluckj | so there's a tradeoff, I guess :P | 10:06 |
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kanzure | "Visualize black hole gravitational lensing in WebGL" https://github.com/cliffcrosland/black-hole.js | 10:20 |
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CheckDavid | Oh I see | 10:36 |
CheckDavid | cluckj: I laugh at women who think they will get very large by lifting weights. They are afraid of.getting too much useless muscle | 10:38 |
CheckDavid | Like you | 10:38 |
CheckDavid | But then I know you are not a women because else you wouldn't even be putting on too much muscle | 10:38 |
CheckDavid | Lol | 10:38 |
CheckDavid | That's the funny thing | 10:39 |
cluckj | O_o | 10:40 |
kanzure | uh... | 10:42 |
kanzure | wat | 10:43 |
cluckj | ^ | 10:43 |
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kanzure | "They had the brain open, and every single time that they had an electrode stimulation to a very certain region of the cardiac nucleuses, which is part of this wanting system, the patient would turn around and say that he loved the doctor." | 10:56 |
andytoshi | that doctor sounds lovely | 10:57 |
kanzure | no you're supposed to wait until he zaps you | 10:58 |
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andytoshi | oh, ok | 10:59 |
andytoshi | i can't wait :] | 10:59 |
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nmz787 | this looks pretty friggin cool http://www.learnopencv.com/seamless-cloning-using-opencv-python-cpp/ | 11:55 |
chris_99 | intriguing | 11:55 |
chris_99 | but i guess it only works if the object has a solid background | 11:56 |
chris_99 | i'm still deciding on the ADC, nmz787, maybe i should just go for something cheap to start with | 12:09 |
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nmz787 | chris_99: have you checked the openspectrometer specs? | 13:01 |
nmz787 | the ADC isn't outlandishly priced | 13:01 |
chris_99 | yeah that seemed a good one, the wolfson one | 13:02 |
chris_99 | if i just used one of those 'slide' diffraction gratings, i can just place it straight in front of the ccd right | 13:03 |
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nmz787 | by slide you mean transmission? | 13:43 |
chris_99 | yeah | 13:43 |
nmz787 | yep, check out the PLOTS spectrometer to see their setup instructions | 13:43 |
chris_99 | ah cheers | 13:43 |
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kanzure | vivi: hi | 14:39 |
vivi | hello kanzure | 14:40 |
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* Adlai , being drunk, is now curious to solicit the channel's opinion of alcohol | 16:17 | |
Adlai | it's definitely not nootropic | 16:17 |
Adlai | subhuman, and by muddling the conscious/precise mind, it interferes with bio- and hardware hacking - thus a perfect fit for this channel! | 16:17 |
kanzure | lobotomies are also not often nootropic | 16:19 |
Adlai | sure, but lobotomies don't write poetry, or cause desperate monkeys to ask other monkeys what they think of monkeydom | 16:20 |
Adlai | (and neither do lobotomized monkeys, they tend to sit around, staring at wallS) | 16:21 |
* Adlai has been pondering the value of a university education | 16:27 | |
Adlai | some of the last words out of my mouth were | 16:27 |
Adlai | "i have found a bunch of people doing independent biology, so even that isn't restricted to the hallowed groves of academe anymore" | 16:28 |
Adlai | prove me wrong, please | 16:28 |
Adlai | (actuallly, i'd rather you don't, but i think you know what you mean. yes, _you_) | 16:28 |
cluckj | :( | 16:30 |
cluckj | me? | 16:30 |
kanzure | Adlai: friends don't let friends go to school | 16:31 |
cluckj | hah | 16:31 |
Adlai | o rly | 16:32 |
Adlai | y not? | 16:32 |
* Adlai means, that the last friends - with whom he spoke about the last words whence his mouth - urged him to study | 16:32 | |
kanzure | learning can happen anywhere, and you can join labs without being a student | 16:32 |
Adlai | and by "study", /me implies at a formal institution | 16:32 |
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kanzure | and besides, you're a programmer. your industry doesn't even care about credentials. | 16:34 |
cluckj | I wonder why that is | 16:35 |
Adlai | eh it only disregards credentials when your github is full of nodejs angular blabla | 16:35 |
* Adlai managed the "i don't have a resume but github" once, twas nice, nevar again | 16:35 | |
kanzure | nobody actually reads source code on github when a candidate applies. they really don't... | 16:35 |
kanzure | (but yes you should still have shit there) | 16:36 |
Adlai | dunno | 16:37 |
Adlai | i heard "he thought your github was nice" in the first skype call | 16:37 |
Adlai | doesn't that _mean_ something? | 16:38 |
kanzure | it could easily mean nothing, like "he said something positive, but i don't understand the gitz, so i am calling you to waste your time anyway instead of just having him review the quality of source code you write" | 16:39 |
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andytoshi | kanzure: actually i received a PM from an employer (of others) complaining that github made it hard to tell whether contributions are "real" | 17:08 |
andytoshi | that is, code rather than docfixes | 17:08 |
andytoshi | so he/she is at least looking as far as what shape are git commits | 17:08 |
andytoshi | but maybe the people who PM me about their hiring troubles are not typical employers. *shrug* | 17:08 |
Adlai | andytoshi: IME, PMs are from recruiters | 17:09 |
Adlai | (for values of personal increasingly tending towards "linked") | 17:09 |
andytoshi | Adlai: fyi, people keep irc logs forever ... it's kinda fun but i make a point of never being on IRC while drunk for that reason | 17:11 |
andytoshi | (not that you've said anything worrisome, just general advice) | 17:11 |
* Adlai has seen far worse | 17:11 | |
Adlai | some IRC networks are built around drugs. imagine how #stims on irc.tripsit.me looks... | 17:12 |
andytoshi | sure, but if you're looking to do research/development stuff under this name with people on this network, there's a benefit to being more cautious. *shrug again* | 17:13 |
kanzure | andytoshi: git commits can be forged too, but whatever | 17:13 |
* Adlai shuts up? | 17:14 | |
andytoshi | kanzure: this is true, but i think the skill needed to realize that is also a barrier.. | 17:17 |
andytoshi | idk, maybe it'd be more common if it was more useful | 17:18 |
andytoshi | right now you can post "trololol" in the Issues section of a million projects and github will say you've "contributed to" them all | 17:18 |
Adlai | yes, but forging _history_ is more difficult | 17:18 |
Adlai | (and requires getting pulled, or convincing your potential employers that your nonsense really is worth something) | 17:19 |
andytoshi | Adlai: well, you need push access to whatever repo you want that history to be in...but the actual forgery is easy enough | 17:20 |
andytoshi | like you can just put your name on every commit | 17:20 |
* Adlai has several times told himself that correcting trailing whitespace in widely used open source projects is not worth the github profile boost that it would yield | 17:20 | |
Adlai | please, show me the recruiter than would notice. | 17:21 |
andytoshi | btw kanzure, it took me all of five minutes to set up a new server running modern software (i'm migrating files right now), thx for the push to do it.. | 17:34 |
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kanzure | cool | 17:37 |
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kanzure | "Before bitcoin [ransomware] was done with Moneypack, Western Union, and others" | 17:55 |
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kanzure | somewhat more popularized fermi paradox explanation post http://waitbutwhy.com/2014/05/fermi-paradox.html | 18:46 |
adlai | we have no way of directly inspecting "the multiverse"... maybe "life" (ie consciousness capable of conceiving such paradoxen) is much much rarerererer than expected, and by the time/verse it arises, is alone to wonder about this? | 18:49 |
kanzure | adlai: one interesting theory is that binary pulsars are probably alien civilizatios | 18:49 |
kanzure | alien civilizations | 18:49 |
* adlai hopes that any aliens capable of reaching us with signals would be capable of a) being reached by ours, and b) understanding how to adapt their signals to be parsable by our admittedly humble intelligence | 18:50 | |
adlai | it could also be that "nobody cares", it's a simulation/anomaly/whateveryoumakeofit | 18:51 |
adlai | these are all equivalent, from within. | 18:51 |
adlai | maybe i'm expecting the communication counterparty to slow down for my benefit, a sin equal to expecting your audience to "keep up" (an accusation recently levelled against an author using too much math in a paper intended for mathematicians) | 18:53 |
adlai | ... but why shouldn't non-interplanetary worms on a rock in space hope that their elders give them a helping hand? | 18:54 |
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kanzure | http://www.piratehearts.com/blog/2014/03/28/crt-simulation/ | 19:13 |
streety | paperbot http://www.sciencedirect.com/science/article/pii/S0956566315002018 | 19:18 |
kanzure | .title | 19:18 |
yoleaux | Microfluidic enzymatic biosensing systems: A review | 19:18 |
paperbot | http://diyhpl.us/~bryan/papers2/paperbot/%0A%20Microfluidic%20enzymatic%20biosensing%20systems%3A%20A%20review%0A%20.pdf | 19:19 |
kanzure | paperbot: http://www.sciencedirect.com/science/article/pii/S0956566315002018/pdfft?md5=74aa65b3289041cdf3bdd4154bb8a5fb&pid=1-s2.0-S0956566315002018-main.pdf | 19:19 |
paperbot | http://diyhpl.us/~bryan/papers2/paperbot/6062d8ae911843d1978167384447d54a.pdf | 19:19 |
streety | thanks | 19:21 |
adlai | kanzure: that crt simulation is skeumorphism taken to an impressive/worrying extreme | 19:34 |
kanzure | isn't that the channel moto? "take everything to worrying extremes"/ | 19:35 |
adlai | /motto | 19:35 |
kanzure | ah yes | 19:35 |
* adlai must've lepht something behind | 19:36 | |
kanzure | ugh that person? | 19:36 |
adlai | dunno, you said worrying extreme | 19:36 |
kanzure | lepht is a person | 19:36 |
adlai | who, according to links from /motto, is extreme | 19:37 |
kanzure | uh? | 19:40 |
adlai | well i guess it's not linked from teh topic, but from the wiki. | 19:40 |
kanzure | she's just pandering to a boring audience who don't understand magnets or engineering | 19:42 |
adlai | them's a she now? | 19:43 |
* adlai means no jest, trying to make sense | 19:44 | |
kanzure | lepht is a person | 19:44 |
kanzure | her audience is a group of people | 19:44 |
adlai | ok | 19:45 |
* adlai wonders if any biohackers have tackled sleep yet, or is that left for after the BRAIN initiative | 19:45 | |
kanzure | introducing the most boring tool for scientific pdfs http://www.docear.org/ (really, this makes no sense. sounds super boring.) | 19:59 |
kanzure | .title https://news.ycombinator.com/item?id=9334316 | 20:27 |
yoleaux | The future of the postdoc | Hacker News | 20:27 |
cluckj | tenure or bust | 20:35 |
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