| --- Log opened Wed Jul 22 00:00:18 2015 | ||
| -!- proofoflogic [uid65184@gateway/web/irccloud.com/x-ciwyoaveruobahed] has joined ##hplusroadmap | 00:01 | |
| -!- FourFire [~FourFire@185.7.192.138] has joined ##hplusroadmap | 00:08 | |
| -!- redlegion [~x@unaffiliated/redlegion] has quit [Ping timeout: 256 seconds] | 00:14 | |
| -!- p4nd4 [~quassel@104.219.54.200] has quit [Quit: No Ping reply in 180 seconds.] | 00:14 | |
| -!- p4nd4 [~quassel@104.219.54.200] has joined ##hplusroadmap | 00:14 | |
| -!- redlegion [~x@unaffiliated/redlegion] has joined ##hplusroadmap | 00:16 | |
| -!- redlegion [~x@unaffiliated/redlegion] has quit [Ping timeout: 264 seconds] | 00:47 | |
| -!- p4nd4 [~quassel@104.219.54.200] has quit [Quit: No Ping reply in 180 seconds.] | 00:48 | |
| -!- p4nd4 [~quassel@104.219.54.200] has joined ##hplusroadmap | 00:49 | |
| -!- redlegion [~x@unaffiliated/redlegion] has joined ##hplusroadmap | 00:50 | |
| EnLilaSko | xrr: Google something like "mmol/l to mg/l" | 00:55 |
|---|---|---|
| xrr | EnLilaSko, yeah, thanks. I think I understand the conversion via molar mass. But I'm not sure what mass has been used to calculate the mg/l. Just the atom, or some larger molecule? | 01:38 |
| xrr | https://en.wikipedia.org/wiki/Reference_ranges_for_blood_tests apparently uses just atomic masses. 140 mg / 3.5 mmol = 40 g/mol ~= 39 for potassium atom. But 1750 mg / 3.5 mmol = 500 g/mol is just weird. Well anyways, I don't really care what mass is used, so I could just use 500 g/mol for conversion, but the problem is that 1850/5 = 370 g/mol, which is way different. Yeah, I don't know. Maybe the lab just changed the reference ranges. I'll try to | 01:38 |
| xrr | figure this out. | 01:38 |
| -!- nsh [~lol@wikipedia/nsh] has quit [Excess Flood] | 01:43 | |
| -!- nsh [~lol@wikipedia/nsh] has joined ##hplusroadmap | 01:44 | |
| -!- nsh [~lol@wikipedia/nsh] has quit [Max SendQ exceeded] | 01:44 | |
| -!- nsh [~lol@wikipedia/nsh] has joined ##hplusroadmap | 01:44 | |
| -!- nsh [~lol@wikipedia/nsh] has quit [Max SendQ exceeded] | 01:44 | |
| -!- nsh [~lol@wikipedia/nsh] has joined ##hplusroadmap | 01:45 | |
| tek_wizzard | xrr: why don't you call the lab and ask them | 02:01 |
| xrr | tek_wizzard, I probably should. Thanks. | 02:08 |
| -!- proofoflogic [uid65184@gateway/web/irccloud.com/x-ciwyoaveruobahed] has quit [Quit: Connection closed for inactivity] | 02:27 | |
| -!- nsh [~lol@wikipedia/nsh] has quit [Excess Flood] | 02:36 | |
| -!- nsh [~lol@wikipedia/nsh] has joined ##hplusroadmap | 02:37 | |
| -!- nsh [~lol@wikipedia/nsh] has quit [Max SendQ exceeded] | 02:37 | |
| -!- nsh [~lol@wikipedia/nsh] has joined ##hplusroadmap | 02:37 | |
| -!- nsh [~lol@wikipedia/nsh] has quit [Excess Flood] | 02:39 | |
| -!- nsh [~lol@wikipedia/nsh] has joined ##hplusroadmap | 02:39 | |
| -!- nsh [~lol@wikipedia/nsh] has quit [Excess Flood] | 02:39 | |
| -!- nsh [~lol@wikipedia/nsh] has joined ##hplusroadmap | 02:40 | |
| -!- nsh [~lol@wikipedia/nsh] has quit [Excess Flood] | 02:40 | |
| -!- nsh [~lol@wikipedia/nsh] has joined ##hplusroadmap | 02:40 | |
| -!- nsh [~lol@wikipedia/nsh] has quit [Client Quit] | 02:41 | |
| -!- FourFire [~FourFire@185.7.192.138] has quit [Ping timeout: 244 seconds] | 02:48 | |
| -!- tek_wizzard [~tek_wizza@99-91-0-194.lightspeed.austtx.sbcglobal.net] has quit [Remote host closed the connection] | 03:04 | |
| -!- nsh- [~lol@wikipedia/nsh] has joined ##hplusroadmap | 03:08 | |
| jrayhawk | xrr: Lab reference ranges are not universally defined; typically it's just some percentile of whomever goes through that particular lab. | 03:25 |
| -!- CheckDavid [uid14990@gateway/web/irccloud.com/x-sbfsqvrgcoueskvt] has joined ##hplusroadmap | 03:26 | |
| jrayhawk | This is one of the hobby horses of the Functional Medicine folks. | 03:26 |
| jrayhawk | Getting sane standards into textbooks. | 03:27 |
| jrayhawk | And on charts. | 03:27 |
| jrayhawk | If you want more resources on that, let me know. | 03:28 |
| -!- nsh- is now known as nsh | 03:41 | |
| -!- Viper168 [~Viper@unaffiliated/viper168] has quit [Quit: Leaving] | 04:10 | |
| -!- Viper168 [~Viper@unaffiliated/viper168] has joined ##hplusroadmap | 04:19 | |
| -!- Madplatypus [uid19957@gateway/web/irccloud.com/x-uzqbrrtaknokhrqu] has quit [Quit: Connection closed for inactivity] | 04:36 | |
| abetusk | http://openplant.org/event-directory/open-technology-week/ | 04:44 |
| -!- FourFire [~FourFire@185.7.192.138] has joined ##hplusroadmap | 04:49 | |
| -!- chris_99 [~chris_99@unaffiliated/chris-99/x-3062929] has joined ##hplusroadmap | 05:03 | |
| -!- eudoxia [~eudoxia@r167-57-25-202.dialup.adsl.anteldata.net.uy] has joined ##hplusroadmap | 05:31 | |
| mgin | yo | 05:44 |
| kanzure | yes? | 05:44 |
| mgin | i'm interested in talking with people who seriously want to achieve immortality | 05:45 |
| kanzure | you already said that. if you really wanted that, you would know that serious people are capable of remembering your shit. | 05:48 |
| mgin | well i'm not talking about you | 05:49 |
| kanzure | ah because...? | 05:49 |
| mgin | i haven't even been able to get a straight answer out of you whether you're interested in such a thing, and i don't really want to keep trying | 05:50 |
| kanzure | have you read any of the links i keep giving you? | 05:50 |
| kanzure | also you should read all of this- http://diyhpl.us/~bryan/papers2/longevity/ | 05:53 |
| -!- Acty [uid89656@gateway/web/irccloud.com/x-sevyutpnkwoctbpo] has joined ##hplusroadmap | 05:54 | |
| mgin | i'm mainly interested in talking to people who are serious about achieving immortality right now, so you don't have to keep sending me links | 05:57 |
| kanzure | justanotheruser: ah i didn't know they had released their source code, https://github.com/strib/scigen | 05:57 |
| kanzure | mgin: fuck talking, start figuring it out- you're going to have to read stuff that's not in an irc channel | 05:58 |
| mgin | i don't really appreciate that | 05:58 |
| kanzure | acquiring skills and knowledge is critical to not dying | 06:00 |
| Betawolf | I don't know what you expect to achieve in a conversation with such a person that would be different from reading a resource such as what kanzure just provided. 'Sure, I'll add you to a list for when I've figured it out'? | 06:02 |
| mgin | i'll ask again later | 06:03 |
| kanzure | do you disagree with this approach to not dying? | 06:03 |
| mgin | are you talking to me? | 06:04 |
| kanzure | yes | 06:04 |
| mgin | i don't know what you're referring to | 06:04 |
| kanzure | ..... | 06:05 |
| kanzure | 06:00 < kanzure> acquiring skills and knowledge is critical to not dying | 06:05 |
| mgin | are you seriously telling me that you're "approach" to not dying is to "acquire skills and knowledge", and asking me if i think that's a good "approach"? | 06:06 |
| kanzure | yes; because you've ignored all of the resources i keep telling you to read. | 06:06 |
| kanzure | so from this data it seems that you disagree | 06:07 |
| mgin | your* | 06:08 |
| mgin | I guess I can infer that you are trying to live forever, at least in your own mind | 06:08 |
| mgin | I'm not really interested in talking to you though | 06:08 |
| Betawolf | I see. You're interested in _talking_ to someone who wants to achieve immortality. For an abstract debate of some kind. | 06:09 |
| mgin | is this place infested with morons? jesus christ | 06:10 |
| ebowden_ | lol | 06:10 |
| mgin | I guess in a chat this big it's inevitable | 06:10 |
| ebowden_ | Earlier he was discussing new microfluidic techniques for oglionucleotide synthesis. | 06:11 |
| ebowden_ | *techniques in microfluidics for | 06:11 |
| ebowden_ | He is no fool, though appears to be good at suffering them. | 06:12 |
| mgin | well mentally ill people can be knowledgeable too | 06:13 |
| ebowden_ | I find him unpleasant, but how on earth do you know he's mentally ill? | 06:14 |
| ebowden_ | And you were talking about morons. | 06:14 |
| mgin | whatever the problem is | 06:14 |
| mgin | tired of people with problems | 06:14 |
| ebowden_ | Everyone has problems. | 06:15 |
| mgin | not really. | 06:15 |
| ebowden_ | Who has no problems whatever? | 06:16 |
| mgin | that's a pretty cynical statement | 06:16 |
| ebowden_ | Not really... | 06:17 |
| ebowden_ | http://www.merriam-webster.com/dictionary/problem | 06:17 |
| mgin | look people have goals, they have work, etc. doesn't mean everyone has some kind of personal problem | 06:17 |
| ebowden_ | I said problems. You are adding things to what I said. | 06:18 |
| mgin | what do you guys think of the difference between verbal and technical intelligence? | 06:32 |
| mgin | it's something that i seem to keep noticing over the years, but i don't know of any strong literature on it | 06:32 |
| FourFire | mgin, still around? | 06:59 |
| FourFire | mgin, read this paragraph, for future reference please: https://en.wikipedia.org/wiki/Indefinite_lifespan#Not_immortality | 07:01 |
| FourFire | Oh, had mgin but pinged me I could have answered some of his questions | 07:05 |
| kanzure | it's still not clear to me whether mgin is looking to acquire skills necessary for cellular and molecular biology projects, or if he's more interested in debate. | 07:20 |
| ebowden_ | It does not appear that he is attempting to acquire those skills. | 07:22 |
| kanzure | alright then | 07:28 |
| -!- delinquentme [~delinquen@74.61.157.78] has quit [Read error: Connection reset by peer] | 07:37 | |
| -!- davm_ [~davm@adsl-107-203-26-177.dsl.tul2ok.sbcglobal.net] has quit [Ping timeout: 255 seconds] | 08:01 | |
| kanzure | "A new approach for immobilization of oligonucleotides onto piezoelectric quartz crystal for preparation of a nucleic acid sensor for following hybridization" | 08:19 |
| kanzure | "Oligonucleotide immobilization on micropatterned streptavidin surfaces" eh... requires "silylated silicon surfaces". hm. | 08:20 |
| -!- nottimschmidt [~timschmid@35.10.220.134] has quit [Ping timeout: 244 seconds] | 08:23 | |
| -!- sheena [~home@d162-156-32-180.bchsia.telus.net] has joined ##hplusroadmap | 08:24 | |
| -!- nottimschmidt [~timschmid@35.10.220.134] has joined ##hplusroadmap | 08:24 | |
| kanzure | "The biotin-streptavidin interaction can be reversibly broken using water at elevated temperatures" | 08:26 |
| kanzure | http://www.researchgate.net/profile/Mathias_Uhlen/publication/8042988_The_biotin-streptavidin_interaction_can_be_reversibly_broken_using_water_at_elevated_temperatures/links/00463524e840f34ba9000000.pdf | 08:26 |
| kanzure | "A factorial analysis of silanization conditions for the immobilization of oligonucleotides on glass surfaces" | 08:30 |
| kanzure | http://www.researchgate.net/profile/Tony_Cass/publication/11937016_A_factorial_analysis_of_silanization_conditions_for_the_immobilization_of_oligonucleotides_on_glass_surfaces/links/0deec532837cc36f7b000000.pdf | 08:30 |
| kanzure | hm well i can't seem to find any evidence of a reversible immobilization method | 08:32 |
| CaptHindsight | kanzure: lets just say that we have the magic drop mover and mixer, what's next? | 08:32 |
| CaptHindsight | there are photocleavable linkers to silanes | 08:33 |
| kanzure | next is a magic drop washer that doesn't obliterate the dna or oligonucleotides | 08:33 |
| kanzure | and also, it would be ideal to avoid gel extraction techniques (e.g. "run it through gel electrophoresis, then slice the gel cake, then elute, then centrifuge for a while, then try to manually recover the dna") because they take way too many steps (having a machine do a million gel extractions is ugh) | 08:35 |
| CaptHindsight | oligos bonded to a silane that can be moved and washed | 08:36 |
| kanzure | i think they need to be released for the assembly steps. and once they are released, i don't think any more wash steps are possible. | 08:36 |
| kanzure | .wik gel extraction | 08:37 |
| yoleaux | "In molecular biology, gel extraction or gel isolation is a technique used to isolate a desired fragment of intact DNA from an agarose gel following agarose gel electrophoresis. After extraction, fragments of interest can be mixed, precipitated, and enzymatically ligated together in several simple steps." — https://en.wikipedia.org/wiki/Gel_extraction | 08:37 |
| CaptHindsight | can't they be assembled going in the opposite direction from the base bonded to the silane? | 08:37 |
| kanzure | there are two dna inputs to each assembly operation (well at least 2 dna inputs). if they are both bonded to different objects, then they will never interact. | 08:39 |
| kanzure | what is my magic million-drop drop mover? | 08:40 |
| ParahSailin_ | .title http://www.eetimes.com/author.asp?section_id=36&doc_id=1327192 | 08:40 |
| yoleaux | Why I Won't Do Another Kickstarter Campaign | EE Times | 08:40 |
| ParahSailin_ | from the parallela adapteva guy | 08:40 |
| -!- delinquentme [~delinquen@74.61.157.78] has joined ##hplusroadmap | 08:48 | |
| delinquentme | kanzure, | 08:49 |
| delinquentme | uhwayke? | 08:49 |
| kanzure | whatup | 08:51 |
| delinquentme | gchat | 08:51 |
| FourFire | wait, parallela isn't a dud? | 08:53 |
| FourFire | I though it was since they silently cancelled the 64 core design | 08:53 |
| delinquentme | last I heard there was some kid doing research in parallel compute and wanting to build out a small parallela cluster | 08:54 |
| kanzure | delinquentme: ok replid on gchat | 08:54 |
| FourFire | delinquentme, reference? | 08:58 |
| delinquentme | FourFire, Im wanting to say it was some kid posting this on a crowdfunding site | 09:05 |
| delinquentme | im getting search collisions with parallella though so | 09:05 |
| -!- ahab [~ahab@107.15.99.154] has joined ##hplusroadmap | 09:06 | |
| -!- ebowden_ [~ebowden@147.69.32.113] has quit [Read error: Connection reset by peer] | 09:06 | |
| kanzure | ahab: hello | 09:07 |
| -!- ebowden [~ebowden@147.69.32.113] has joined ##hplusroadmap | 09:07 | |
| ahab | hello kanzure | 09:07 |
| FourFire | delinquentme, well the closest I saw was some silly 64 RPi "supercomputer" made with lego, and a 6 node Odroid-X2 | 09:08 |
| FourFire | I'm interested in any low cost per 16x PCIe 3.0 port | 09:09 |
| FourFire | if there are ARM computers with PCIe x16 then I might have a use for them | 09:09 |
| JayDugger | tACS and lucid dreaming: Induction of self awareness in dreams through frontal low current stimulation of gamma activity, http://www.nature.com/neuro/journal/v17/n6/pdf/nn.3719.pdf | 09:11 |
| kanzure | ahab: what brings you here? | 09:11 |
| ahab | kanzure, interest in the topic of course, but also just to be able to talk to people with shared interests. I'm relatively new in my town and an introvert, so this is my attempt to be a bit more social. | 09:13 |
| kanzure | you seem to do bitcoin things | 09:16 |
| delinquentme | ^ the master at work | 09:19 |
| delinquentme | ahab, this is the place to be | 09:19 |
| delinquentme | IDK if we deliver on work as often as we like | 09:20 |
| ahab | kanzure, if that statement was directed at me....wow...yeah I do some bitcoin stuff. | 09:20 |
| delinquentme | but its quite agood spot for novel ideas / vetting of concepts in hard science | 09:20 |
| kanzure | ahab: it was :-) | 09:21 |
| ahab | delinquentme, that is what I am more interested in, I used to do research in nanomaterials | 09:21 |
| kanzure | which materials? | 09:21 |
| ahab | kanzure, well...I have 34 asics on standby atm... | 09:21 |
| ahab | OMC - ordered mesoporous carbon | 09:21 |
| kanzure | ahab: use nicehash.com | 09:21 |
| ahab | kanzure, I'll check it out | 09:22 |
| kanzure | ahab: we're building an inkjet dna synthesizer (phosphoramidite chemistry) | 09:22 |
| kanzure | a material with 50 nm pores would be very useful, especially if they have bottoms | 09:22 |
| ahab | kanzure, I can create an ordered hexagonal matrix of either amorphous/crystalline carbon and almost any pore size...well I used to | 09:23 |
| kanzure | ahab: http://diyhpl.us/wiki/dna/synthesis/notes/ | 09:24 |
| delinquentme | ahab, why didnt you get here sooner | 09:25 |
| ahab | deliquentme...well, the introvert part...but I'm here now | 09:26 |
| ahab | kanzure, can you see academic pulications? | 09:26 |
| ahab | JACS, Advanced functional materials? | 09:26 |
| kanzure | haven't checked those, but yea i have access to some stuff | 09:31 |
| ahab | creating ordered mesoporous carbon is quite easy, use block copolymers for self assembly and depending on the ratios and or added materials, you can dictate pore size | 09:33 |
| -!- sheena [~home@d162-156-32-180.bchsia.telus.net] has quit [Ping timeout: 256 seconds] | 09:37 | |
| ahab | brb | 09:40 |
| delinquentme | ahab, mesoporous ... can you explain this term? | 09:44 |
| JayDugger | medium-sized pores in carbon, but that leaves a lot to be desired as a defintion. | 09:49 |
| -!- eudoxia [~eudoxia@r167-57-25-202.dialup.adsl.anteldata.net.uy] has quit [Quit: Leaving] | 09:53 | |
| CaptHindsight | I have to run but you bond the first base to the silane and grow that to a desire length and then cleave the bond to the silane | 09:55 |
| CaptHindsight | the free oligo may now be bonded an oligo still bonded on one end to the silane | 09:56 |
| CaptHindsight | + to | 09:56 |
| kanzure | once you cleave, how do you bond it back? | 09:56 |
| CaptHindsight | you won't bond it back to the silane, just to an oligo that is still anchored | 09:57 |
| kanzure | how will you bond it to an oligo that is still anchored? | 09:57 |
| CaptHindsight | have to run | 09:58 |
| CaptHindsight | but I'll go over it later | 09:58 |
| -!- delinquentme [~delinquen@74.61.157.78] has quit [Quit: Leaving] | 09:59 | |
| kanzure | haha ancestry.com is expanding into healthcare looolz | 10:09 |
| -!- FourFire [~FourFire@185.7.192.138] has quit [Ping timeout: 244 seconds] | 10:13 | |
| -!- Viper168 [~Viper@unaffiliated/viper168] has quit [Ping timeout: 244 seconds] | 10:16 | |
| -!- FourFire [~FourFire@125-26-11.connect.netcom.no] has joined ##hplusroadmap | 10:24 | |
| -!- FourFire [~FourFire@125-26-11.connect.netcom.no] has quit [Ping timeout: 244 seconds] | 10:31 | |
| -!- FourFire [~FourFire@53-219-11.connect.netcom.no] has joined ##hplusroadmap | 10:43 | |
| -!- delinquentme [~delinquen@74.61.157.78] has joined ##hplusroadmap | 11:02 | |
| -!- ebowden [~ebowden@147.69.32.113] has quit [Ping timeout: 246 seconds] | 11:23 | |
| -!- ebowden [~ebowden@147.69.32.113] has joined ##hplusroadmap | 11:38 | |
| -!- eudoxia [~eudoxia@r167-57-25-202.dialup.adsl.anteldata.net.uy] has joined ##hplusroadmap | 11:41 | |
| kanzure | bloop | 11:42 |
| -!- EnLilaSko [~Nattzor@unaffiliated/enlilasko] has quit [Ping timeout: 246 seconds] | 11:51 | |
| -!- EnLilaSko [~Nattzor@host-85-30-145-65.sydskane.nu] has joined ##hplusroadmap | 11:51 | |
| -!- EnLilaSko [~Nattzor@host-85-30-145-65.sydskane.nu] has quit [Changing host] | 11:51 | |
| -!- EnLilaSko [~Nattzor@unaffiliated/enlilasko] has joined ##hplusroadmap | 11:51 | |
| -!- PatrickRobotham [uid18270@gateway/web/irccloud.com/x-xzasxtukayaqyvhu] has quit [Quit: Connection closed for inactivity] | 11:58 | |
| -!- ebowden [~ebowden@147.69.32.113] has quit [Ping timeout: 264 seconds] | 12:08 | |
| -!- Madplatypus [uid19957@gateway/web/irccloud.com/x-orxtnusqlnngegxn] has joined ##hplusroadmap | 12:16 | |
| delinquentme | kanzure, | 12:27 |
| delinquentme | other than epPCR. What are other meaningful ways to add meaningful mutations to an organisms genome | 12:28 |
| delinquentme | are there liquid handling methods which could do such a thing? | 12:28 |
| kanzure | .wik site-specific mutagenesis | 12:28 |
| yoleaux | "Site-directed mutagenesis is a molecular biology method that is used to make specific and intentional changes to the DNA sequence of a gene and any gene products." — https://en.wikipedia.org/wiki/Site-specific_mutagenesis | 12:28 |
| -!- QuadIngi [~FourFire@53-219-11.connect.netcom.no] has joined ##hplusroadmap | 12:30 | |
| -!- Madplatypus is now known as Maddragon | 12:32 | |
| -!- FourFire [~FourFire@53-219-11.connect.netcom.no] has left ##hplusroadmap ["Leaving"] | 12:34 | |
| kanzure | ugh http://futuregrind.org/2015/07/15/ep-1-tim-cannon-on-biohacking-and-transhumanism/ | 12:34 |
| -!- QuadIngi is now known as FourFire | 12:36 | |
| delinquentme | would raman spec be useful on cells to determine if they're expressing novel proteins ? or is that env just too non-homogenous? | 12:38 |
| delinquentme | say I have baseline cell gen1, i change shit, get gen2, I want to compare + see if expression of a known protein was upregulated. I know this can be done w sequencing/ metabolomics | 12:39 |
| -!- erasmus [~esb@unaffiliated/erasmus] has joined ##hplusroadmap | 12:56 | |
| -!- rigel [~yourmom@c-24-21-52-83.hsd1.or.comcast.net] has quit [Read error: Connection reset by peer] | 13:13 | |
| -!- rigel [~yourmom@c-24-21-52-83.hsd1.or.comcast.net] has joined ##hplusroadmap | 13:13 | |
| -!- justanotheruser [~Justan@unaffiliated/justanotheruser] has quit [Read error: Connection reset by peer] | 13:16 | |
| -!- justanotheruser [~Justan@unaffiliated/justanotheruser] has joined ##hplusroadmap | 13:16 | |
| -!- Maddragon is now known as Madplatypus | 13:25 | |
| -!- rigel [~yourmom@c-24-21-52-83.hsd1.or.comcast.net] has quit [Read error: Connection reset by peer] | 13:32 | |
| -!- rigel [~yourmom@c-24-21-52-83.hsd1.or.comcast.net] has joined ##hplusroadmap | 13:32 | |
| -!- rigel [~yourmom@c-24-21-52-83.hsd1.or.comcast.net] has quit [Read error: Connection reset by peer] | 13:36 | |
| -!- rigel [~yourmom@c-24-21-52-83.hsd1.or.comcast.net] has joined ##hplusroadmap | 13:37 | |
| -!- rigel [~yourmom@c-24-21-52-83.hsd1.or.comcast.net] has quit [Read error: Connection reset by peer] | 13:41 | |
| -!- rigel [~yourmom@c-24-21-52-83.hsd1.or.comcast.net] has joined ##hplusroadmap | 13:42 | |
| -!- rigel [~yourmom@c-24-21-52-83.hsd1.or.comcast.net] has quit [Read error: Connection reset by peer] | 13:47 | |
| -!- rigel [~yourmom@c-24-21-52-83.hsd1.or.comcast.net] has joined ##hplusroadmap | 13:47 | |
| -!- eudoxia [~eudoxia@r167-57-25-202.dialup.adsl.anteldata.net.uy] has quit [Quit: Leaving] | 14:05 | |
| -!- rigel [~yourmom@c-24-21-52-83.hsd1.or.comcast.net] has quit [Read error: Connection reset by peer] | 14:08 | |
| -!- rigel [~yourmom@c-24-21-52-83.hsd1.or.comcast.net] has joined ##hplusroadmap | 14:09 | |
| -!- rigel [~yourmom@c-24-21-52-83.hsd1.or.comcast.net] has quit [Read error: Connection reset by peer] | 14:10 | |
| -!- nottimschmidt [~timschmid@35.10.220.134] has quit [Ping timeout: 244 seconds] | 14:11 | |
| -!- nottimschmidt [~timschmid@35.10.220.134] has joined ##hplusroadmap | 14:13 | |
| -!- rigel [~yourmom@c-24-21-52-83.hsd1.or.comcast.net] has joined ##hplusroadmap | 14:14 | |
| -!- Viper168 [~Viper@unaffiliated/viper168] has joined ##hplusroadmap | 14:19 | |
| -!- drewbot_ [~cinch@ec2-54-82-14-89.compute-1.amazonaws.com] has quit [Remote host closed the connection] | 14:36 | |
| -!- drewbot [~cinch@ec2-54-242-1-227.compute-1.amazonaws.com] has joined ##hplusroadmap | 14:36 | |
| -!- nottimschmidt [~timschmid@35.10.220.134] has quit [Ping timeout: 256 seconds] | 14:37 | |
| -!- nottimschmidt [~timschmid@35.10.220.134] has joined ##hplusroadmap | 14:39 | |
| kanzure | origin of hyperlinks http://hapgood.us/2015/07/21/beyond-conversation/ | 14:40 |
| kanzure | ( https://news.ycombinator.com/item?id=9929187 ) | 14:41 |
| kanzure | 2 mm diameter mirror https://www.youtube.com/watch?v=dkrbR_6_Jx4 | 14:53 |
| kanzure | electronically-addressable piston-based hydraulic actuator http://wims2.org/publications/papers/1308062274-Kim%20Najafi%20MEMS%2009.pdf (but not very tiny?) | 15:08 |
| kanzure | "A method for pneumatically inserting an array of penetrating electrodes into cortical tissue" http://www.cnbc.cmu.edu/~samondjm/papers/RouscheandNormann1992.pdf | 15:20 |
| kanzure | "The goal of this research was to find a practical means by which an array of 100 needle-shaped electrodes could be implanted into the cerebral cortex with minimal brain tissue trauma. It was found that insertion of these structures into cortical tissues could only be performed using high insertion speeds. A pneumatically actuated impact insertion system has been developed that is capable of inserting an electrode array into feline brain ... | 15:22 |
| kanzure | ... tissue at speeds from about 1 to 11 m/sec. We found that a minimum array insertion speed of 8.3 m/sec was necessary for a complete, safe insertion of all 100 electrodes in the array to a depth of 1.5 mm into feline cortex." | 15:22 |
| -!- eudoxia [~eudoxia@r186-55-160-13.dialup.adsl.anteldata.net.uy] has joined ##hplusroadmap | 15:39 | |
| -!- eudoxia [~eudoxia@r186-55-160-13.dialup.adsl.anteldata.net.uy] has quit [Quit: Leaving] | 15:51 | |
| -!- FourFire [~FourFire@53-219-11.connect.netcom.no] has quit [Quit: Leaving] | 16:00 | |
| -!- eudoxia [~eudoxia@r186-55-160-13.dialup.adsl.anteldata.net.uy] has joined ##hplusroadmap | 16:06 | |
| -!- eudoxia [~eudoxia@r186-55-160-13.dialup.adsl.anteldata.net.uy] has quit [Quit: Leaving] | 16:17 | |
| -!- yashgaroth [~ffffff@2602:306:35fa:d500:4935:8e30:c5ff:5d1d] has joined ##hplusroadmap | 16:23 | |
| -!- Viper168_ [~Viper@unaffiliated/viper168] has joined ##hplusroadmap | 16:26 | |
| -!- Viper168 [~Viper@unaffiliated/viper168] has quit [Ping timeout: 240 seconds] | 16:28 | |
| -!- Viper168_ is now known as Viper168 | 16:39 | |
| -!- chris_99 [~chris_99@unaffiliated/chris-99/x-3062929] has quit [Remote host closed the connection] | 16:52 | |
| -!- PatrickRobotham [uid18270@gateway/web/irccloud.com/x-hdqcnrhhobccgfdp] has joined ##hplusroadmap | 17:44 | |
| mgin | hey | 17:51 |
| mgin | alright, variation, who here is trying to live forever, and what's your strategy? | 17:51 |
| kanzure | 1) acquire all knowledge 2) build things http://diyhpl.us/wiki/declaration (there has been no particular criticism with this approach, so in general you can safely assume most people in here agree with this method) | 17:53 |
| mgin | i want to hear others' approach | 17:54 |
| kanzure | well there's the kurzweil approach ("invest in some stocks, sit back and wait 30 years") | 17:56 |
| kanzure | what's your alternative? | 17:57 |
| mgin | well the obvious answer is SENS | 17:58 |
| kanzure | you mean "wait for the sens foundation to do more stuff" or do you mean "i have some plans for working on the sens goals"? | 18:02 |
| mgin | well right, the content of the strategy would be to act in such a way as to maximize one's contribution to that effort | 18:04 |
| CaptHindsight | kanzure: linkers like these are cleaveable leaving blunt ends on the oligos http://www.genelink.com/newsite/products/mod_detail.asp?modid=133 | 18:09 |
| CaptHindsight | Custom Oligonucleotide Synthesis http://www.genelink.com/newsite/products/unmodoligosINTRO.asp Gene Link Oligo Synthesis Division is not an "oligo factory". | 18:15 |
| CaptHindsight | so what are they actually making? | 18:15 |
| CaptHindsight | just mods? | 18:15 |
| streety | kanzure: or haven't read it | 18:18 |
| * streety has only just now read it | 18:18 | |
| -!- delinquentme [~delinquen@74.61.157.78] has quit [Ping timeout: 256 seconds] | 18:45 | |
| mgin | so | 18:55 |
| mgin | is anyone here into SENS? | 18:55 |
| CaptHindsight | Saudi Environmental Society? | 18:56 |
| CaptHindsight | or the Japanese instrumental group? | 18:57 |
| mgin | no | 18:57 |
| mgin | strategies for engineering negligible senesence | 18:58 |
| -!- rigel [~yourmom@c-24-21-52-83.hsd1.or.comcast.net] has quit [Read error: Connection reset by peer] | 19:24 | |
| -!- rigel [~yourmom@c-24-21-52-83.hsd1.or.comcast.net] has joined ##hplusroadmap | 19:25 | |
| -!- rigel [~yourmom@c-24-21-52-83.hsd1.or.comcast.net] has quit [Read error: Connection reset by peer] | 19:26 | |
| -!- rigel [~yourmom@c-24-21-52-83.hsd1.or.comcast.net] has joined ##hplusroadmap | 19:30 | |
| kanzure | mgin: yes, we have some people that used to work at the sens foundation | 19:38 |
| kanzure | CaptHindsight: some of the mailorder dna synthesis companies just have lots and lots of machine to make up for their poor throughput. also they just sequence everything. | 19:39 |
| kanzure | *poor yield | 19:39 |
| -!- CheckDavid [uid14990@gateway/web/irccloud.com/x-sbfsqvrgcoueskvt] has quit [Quit: Connection closed for inactivity] | 19:40 | |
| justanotheruser | mgin: my strategy is to make money while aquiring knowledge so I can build things that lead to immortality | 19:42 |
| kanzure | gasp | 19:43 |
| justanotheruser | I'm a bit caught up in the aquiring money part atm though :D | 19:44 |
| -!- erasmus [~esb@unaffiliated/erasmus] has quit [Ping timeout: 255 seconds] | 19:46 | |
| kanzure | yes well you spend all of your time in school | 19:46 |
| justanotheruser | it is abit strange though, I'm not sure what he's expecting, it's not as if the optimal strategy is mapped out step by step, hplus is full of people making maps of a foggy forest | 19:46 |
| -!- erasmus [~esb@unaffiliated/erasmus] has joined ##hplusroadmap | 19:48 | |
| mgin | justanotheruser: build what things? | 19:49 |
| justanotheruser | mgin: I'm mostly following others leads right now, but I'd like to branch off and work on item two in proposals when I gain sufficient knowledge and equipment | 19:50 |
| mgin | item 2? | 19:50 |
| justanotheruser | http://diyhpl.us/wiki/projects/proposals/ | 19:50 |
| mgin | you're trying to build a cheap atomic microscope? | 19:51 |
| justanotheruser | I plan to try to | 19:51 |
| justanotheruser | I'm still a few steps behind | 19:51 |
| mgin | why? | 19:51 |
| juri_ | I'm studying like crazy, and writing/building free software/hardware. | 19:51 |
| kanzure | there's not much to it, you're going to be disappointed, justanotheruser | 19:51 |
| juri_ | refurbing an SEM. | 19:51 |
| kanzure | it's like a piezo and some tungsten tips or something | 19:51 |
| kanzure | .. and lots of granite. | 19:51 |
| justanotheruser | kanzure: I've looked into it, I remember you telling me that I shouldn't be looking into piezo tubes and piezo buzzers would suffice. Why is that? Do piezo buzzers have the granularity needed? | 19:52 |
| kanzure | tubes.. hm. | 19:52 |
| justanotheruser | I looked into purchasing piezo tubes, but they require a bulk order worth thousands of dollars from the places I checked | 19:52 |
| kanzure | iirc most piezoelectric crystals have the same response properties. | 19:53 |
| mgin | justanotheruser: why? | 19:55 |
| justanotheruser | mgin: because atomic manipulation | 19:55 |
| mgin | can you please explain | 19:56 |
| mgin | because you sound like you're delusional | 19:56 |
| justanotheruser | kanzure: these are 10cm/V https://www.americanpiezo.com/standard-products/buzzers.html | 19:57 |
| justanotheruser | the piezotubes are more like 10nm/V | 19:57 |
| justanotheruser | at least the PZT-5A BM500 PZT-5H BM527 PZT-4 piezotubes are | 19:57 |
| justanotheruser | Doesn't say anything about their consistency at that level, but I'm sure they're not meant to adjust on the scale of microvolts | 19:58 |
| justanotheruser | mgin: What is there to explain? | 19:58 |
| justanotheruser | s/adjust/expand/ | 19:59 |
| kanzure | justanotheruser: hmm well maybe we should do a group buy at some point | 19:59 |
| kanzure | or i'll just buy it and we can split it up (i'm sure nmz787 would like some) | 19:59 |
| kanzure | probably can find on alibaba | 19:59 |
| justanotheruser | yes, in 2013 I didn't try alibaba | 20:00 |
| justanotheruser | but sensortech.ca requires $1k | 20:00 |
| justanotheruser | and I didn't want to buy 5 piezotubes when I needed one | 20:00 |
| mgin | you could answer my question | 20:00 |
| mgin | why are you trying to build a cheap atomic microscope | 20:00 |
| justanotheruser | 22:55 < justanotheruser> mgin: because atomic manipulation | 20:00 |
| mgin | that doesn't mean anything | 20:01 |
| kanzure | haha | 20:01 |
| kanzure | atoms. movement. | 20:01 |
| mgin | right... | 20:01 |
| justanotheruser | !g AFM | 20:01 |
| mgin | "zebras. chairs" | 20:01 |
| justanotheruser | mgin: google what an STM is | 20:01 |
| kanzure | .wik scanning tunneling microscope | 20:02 |
| yoleaux | "A scanning tunneling microscope (STM) is an instrument for imaging surfaces at the atomic level. Its development in 1981 earned its inventors, Gerd Binnig and Heinrich Rohrer (at IBM Zürich), the Nobel Prize in Physics in 1986. For an STM, good resolution is considered to be 0.1 nm lateral resolution and 0.01 nm depth resolution." — https://en.wikipedia.org/wiki/Scanning_tunneling_microscope | 20:02 |
| kanzure | .wik atomic force microscope | 20:02 |
| yoleaux | "Atomic force microscopy (AFM) or scanning force microscopy (SFM) is a very high-resolution type of scanning probe microscopy (SPM), with demonstrated resolution on the order of fractions of a nanometer, more than 1000 times better than the optical diffraction limit." — https://en.wikipedia.org/wiki/Atomic_force_microscope | 20:02 |
| mgin | i know what a microscope is. i'm asking why you are trying to build a cheap one | 20:02 |
| justanotheruser | what is hard to understand about this | 20:02 |
| mgin | why you would want to build a cheap atomic microscope | 20:03 |
| justanotheruser | atomic manipulation has many leads for transhumanism | 20:03 |
| mgin | hence why i asked several times | 20:03 |
| mgin | what does a microscope have to do with manipulation? | 20:03 |
| kanzure | it physically touches things | 20:03 |
| mgin | so? | 20:03 |
| mgin | have you guys even thought this through before? | 20:03 |
| justanotheruser | yes, this is where the disconnect is, it isn't an optical microscope, like I said, google what an STM is, some can be designed to manipulate atoms | 20:03 |
| kanzure | humans do not normally have the ability to manipulate individual atoms | 20:03 |
| mgin | the inability to answer the most rudimentary questions about why you are doing something is a gigantic red flag | 20:04 |
| mgin | it's like you have no clue | 20:04 |
| kanzure | or the other theory is something like "you are incapable of reading" | 20:04 |
| kanzure | given that we have had multiple problems with you by now related to reading this channel, i'm inclined to believe this is the same | 20:05 |
| mgin | i already know you have no clue kanzure, i'm not talking to you | 20:05 |
| mgin | hint: i'm never talking to you | 20:05 |
| kanzure | narf | 20:05 |
| justanotheruser | mgin: so which of these things are you confused about? The fact that atomic manipulation is a lead for transhumanism? The fact that an STM can be used to manipulate atoms? The fact that I want to build one? | 20:06 |
| mgin | those are the three questions on the table, yes | 20:06 |
| justanotheruser | I don't see what isn't clear | 20:06 |
| mgin | you just named them | 20:06 |
| justanotheruser | The questions are each answered by the prior question. I want to build one because it can manipulate atoms. I want to manipulate atoms because the ability to do so is a lead for transhumanism. I admit, my sentence doesn't have an answer for why I want transhumanism though. | 20:07 |
| kanzure | justanotheruser: well not dying is generally useful, so there's that | 20:07 |
| -!- PatrickRobotham [uid18270@gateway/web/irccloud.com/x-hdqcnrhhobccgfdp] has quit [Quit: Connection closed for inactivity] | 20:08 | |
| mgin | how and how? | 20:08 |
| justanotheruser | how does an stm manipulate atoms? I think wikipedia answers that. How do I build one? I like e-bastelns build | 20:08 |
| kanzure | mgin: often projects are useful on their own without any additional purpose in advance; working on hard projects is useful because those skills often translate to very similar problems. so even if his atomic microscope is never used for molecular nanotechnology tooltip shit, it might still be useful for improving his understanding of signal analysis. | 20:09 |
| mgin | well it's just a token question at this point. obviously whatever mental illness kanzure has you share | 20:09 |
| kanzure | mgin: yes, i've basically infected everyone in here | 20:09 |
| justanotheruser | heh | 20:09 |
| kanzure | welcome to the bee hive | 20:09 |
| justanotheruser | I think my trolldar is broken | 20:10 |
| kanzure | justanotheruser: his heart is in the right place, he's just bad at reading or communication or somethikng | 20:10 |
| CaptHindsight | you're either with user agin us :) | 20:10 |
| CaptHindsight | user/ us er | 20:11 |
| CaptHindsight | (spoken with a pirate voice) | 20:11 |
| kanzure | heh | 20:11 |
| CaptHindsight | the pissing contests tends to end when people reach their early 30's, well used to | 20:13 |
| justanotheruser | CaptHindsight: ever been on IRC? | 20:14 |
| CaptHindsight | too long | 20:14 |
| kanzure | what was your magic droplet moving method? | 20:15 |
| drethelin | very well trained butterfly probosci | 20:15 |
| kanzure | go on | 20:17 |
| CaptHindsight | drethelin: with Groucho glasses and mustache | 20:17 |
| drethelin | the moustache turned out to decrease efficiency 34% so we got rid of it | 20:18 |
| CaptHindsight | http://www.jmu.edu/news/madisonscholar/2012POSaM-project.shtml 2012 POSaM respin | 20:20 |
| kanzure | yep i have seen that video | 20:21 |
| CaptHindsight | http://www.protomatix.com/PORTFOLIO/SYSTEMSBIOLOGY/portfolio_isb.html more pics | 20:25 |
| justanotheruser | Expensive ink | 20:25 |
| CaptHindsight | TIJ ink is >$3k per liter when purchased in 10ml cartridges | 20:26 |
| kanzure | ParahSailin_: do you know any fancypants techniques to reattach oligos to solid supports? | 20:28 |
| drethelin | is there any practical way to find out if a product with "0" grams carbohydrate per serving has something like 0.5 or more like 0.001? | 20:29 |
| ParahSailin_ | have them biotinylated | 20:32 |
| -!- erasmus [~esb@unaffiliated/erasmus] has quit [Quit: Namaste] | 20:32 | |
| CaptHindsight | kanzure: why reattach oligos to a surface? | 20:52 |
| CaptHindsight | aren't they grown bonded to something then cleaved and attached to the end of another oligo? | 20:54 |
| kanzure | because wash steps | 21:07 |
| -!- delinquentme [~delinquen@74.61.157.78] has joined ##hplusroadmap | 21:20 | |
| -!- delinquentme [~delinquen@74.61.157.78] has quit [Ping timeout: 240 seconds] | 21:28 | |
| -!- delinquentme [~delinquen@74.61.157.78] has joined ##hplusroadmap | 21:32 | |
| -!- delinquentme [~delinquen@74.61.157.78] has quit [Ping timeout: 240 seconds] | 21:44 | |
| -!- delinquentme [~delinquen@74.61.157.78] has joined ##hplusroadmap | 21:51 | |
| -!- delinquentme [~delinquen@74.61.157.78] has quit [Ping timeout: 250 seconds] | 22:00 | |
| -!- yashgaroth [~ffffff@2602:306:35fa:d500:4935:8e30:c5ff:5d1d] has quit [Quit: Leaving] | 22:16 | |
| -!- juri_ [~juri@vpn166.sdf.org] has quit [Ping timeout: 260 seconds] | 22:24 | |
| -!- Viper168 [~Viper@unaffiliated/viper168] has quit [Quit: Leaving] | 22:25 | |
| -!- Viper168 [~Viper@unaffiliated/viper168] has joined ##hplusroadmap | 22:32 | |
| kanzure | yawn | 22:52 |
| kanzure | http://www.philipmetzger.com/blog/affordable-rapid-bootstrapping-space-industry-solar-system-civilization/ | 23:20 |
| -!- Acty [uid89656@gateway/web/irccloud.com/x-sevyutpnkwoctbpo] has quit [Quit: Connection closed for inactivity] | 23:20 | |
| -!- Porb [~Porbus@101.160.129.140] has joined ##hplusroadmap | 23:23 | |
| -!- Porb is now known as Annnn | 23:23 | |
| drethelin | "affordable" "rapid" | 23:26 |
| drethelin | I love when I don't need to read past the URL to know a post is probably bullshit | 23:26 |
| kanzure | heh | 23:27 |
| kanzure | drethelin: have you read tmp2? http://tmp2.wikia.com/wiki/Main_Page | 23:28 |
| drethelin | I feel like I skimmed it | 23:28 |
| drethelin | but forever ago | 23:28 |
| drethelin | so I'm sure there's a ton more content now | 23:28 |
| kanzure | depends on what your time dilation factor is | 23:28 |
| drethelin | it's like a scifi version of that wiki | 23:29 |
| drethelin | that's full of the simplest, cheapest versions of a lot of machines | 23:29 |
| drethelin | like tractors and generators | 23:29 |
| drethelin | for the third world | 23:29 |
| drethelin | which I can't google because wiki is a uselss search term | 23:29 |
| kanzure | appropedia? | 23:30 |
| drethelin | this looks like a good one though the one I remember had more 3d graphics mockups than photos | 23:31 |
| kanzure | gvcs wiki? http://opensourceecology.org/wiki/ | 23:31 |
| drethelin | ahh that's the one | 23:31 |
| -!- Annnn [~Porbus@101.160.129.140] has quit [Ping timeout: 250 seconds] | 23:48 | |
| -!- sheena [~home@d99-199-117-38.bchsia.telus.net] has joined ##hplusroadmap | 23:53 | |
| --- Log closed Thu Jul 23 00:00:19 2015 | ||
Generated by irclog2html.py 2.15.0.dev0 by Marius Gedminas - find it at mg.pov.lt!