| --- Log opened Sun May 29 00:00:14 2016 | ||
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| jrayhawk | http://time.com/money/4350788/west-virginia-town-for-sale-1-million/ who wants a ready-made cult compound? | 01:11 |
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| kanzure | maybe me, thanks | 03:05 |
| kanzure | no no, wait, i said i would work on this | 03:06 |
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| FourFire | kanzure, the supposed nootropic "Synapsyl" is all bullshit right? | 04:11 |
| FourFire | Yeah, it's a scam. | 04:15 |
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| maaku_ | kanzure: no wifi :( | 06:21 |
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| justanotheruser | oh no kanzure! | 06:38 |
| justanotheruser | github is removing streaks | 06:38 |
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| kanzure | "Google paid $500,000,000 to DoJ to avoid felony charges after they were caught knowingly selling AdWords to an FBI undercover operation pretending to be a Mexican drug lord trying to take over the US steroids market" http://searchengineland.com/wsj-government-sting-google-pharma-ads-109247 | 07:34 |
| maaku_ | can i get a tax refund from that? | 07:44 |
| kanzure | yes but only upon presentation of a valid steroid receipt | 07:48 |
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| maaku_ | http://www.openphilanthropy.org/focus/global-catastrophic-risks/potential-risks-advanced-artificial-intelligence/what-should-we-learn-past-ai-forecasts | 09:51 |
| maaku_ | .title | 09:51 |
| yoleaux | What should we learn from past AI forecasts? | Open Philanthropy Project | 09:51 |
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| kanzure | http://goodcomics.comicbookresources.com/wp-content/uploads/2009/02/flashbomb1.jpg | 11:18 |
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| maaku_ | Their flesh torn apart by nuclear reactions as particles of air impact their skin at relativistic velocities | 11:37 |
| maaku_ | https://what-if.xkcd.com/1/ | 11:37 |
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| kanzure | maaku_: no you see, there was also a molecular demon sorting out any air particles not at the right velocities | 11:59 |
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| jcorgan | in some tiny fraction of the multiverse they just quantum tunneled away | 12:36 |
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| kanzure | oh i mean maxwell's demon i guessw | 12:53 |
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| maaku_ | Right makes sense. Carry on. | 13:14 |
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| kanzure | received this from some bcc-only email a moment ago, | 15:45 |
| kanzure | http://diyhpl.us/~bryan/papers2/DNA/A%20hypothetical%20protocol%20for%20DIYBIO%20DNA%20synthesis.pdf | 15:45 |
| kanzure | yashgaroth: ^ | 15:45 |
| yashgaroth | ok 1) the spelling and grammar omg 2) type IIS restriction enzymes require a double-stranded target at the cleavage site, and while he types a page-long paragraph about getting around that, it's still a clusterfuck to generate ssDNA oligos 3) idk why he suggests PAGE for purification and not HPLC but that's not a major issue [...] | 16:00 |
| yashgaroth | I mean if the TDT stuff works for generating template then great let's go with that, but I figured the much easier way was to generate template with that and then use a nicking enzyme + polymerase like I've said before | 16:01 |
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| kanzure | yashgaroth: i would like some assistance on finding a chemist person to help with the project. i can pay. | 16:10 |
| yashgaroth | hey if the TDT thing works you don't even need a chemist | 16:11 |
| kanzure | er.. | 16:16 |
| yashgaroth | well it's entirely enzymatic aside from the thermolabile nucleotides, which can't be that hard, and I guess the template conjugation to the surface, but just buy biotinylated oligos like w/e | 16:18 |
| kanzure | well the chemist is not just for the chemistry it's for the endless tweaking that literally all biology projects require | 16:20 |
| yashgaroth | the guy who wrote this is a chemist, no? I assume he's not a biologist, since the stuff he writes about hairpins and methylated DNA would be very disappointing | 16:23 |
| kanzure | mathematician | 16:24 |
| kanzure | topoogically complex curve ball, isn't this | 16:24 |
| yashgaroth | that explains a lot | 16:24 |
| yashgaroth | just saying, you may need a tweaker but not necessarily a chemist, optimizing and troubleshooting is most of most bio jobs | 16:26 |
| kanzure | yeah good point, i don't see a strong reason to prefer a *chemist* | 16:27 |
| kanzure | wait why isn't it you | 16:29 |
| yashgaroth | I should know in a couple weeks whether I'm available, since that thing in Seattle may have been funded, in which case I'll need to drop everything | 16:30 |
| nmz787 | kanzure: well they obviously need to do this in microfluidics for the lack of yield on non-porous glass issue | 16:30 |
| nmz787 | I heard the whatever-labile nucleotides were expensive, last time i checked years ago | 16:31 |
| nmz787 | http://www.trilinkbiotech.com/cart/scripts/prodView.asp?idproduct=4665 | 16:37 |
| nmz787 | .title | 16:37 |
| yoleaux | CleanAmp dNTP Set | 16:37 |
| nmz787 | 2 µmoles each (4 x 2) ($84.00) --- 10 µmoles each (4 x 10) ($310) | 16:37 |
| yashgaroth | if you're only generating a template array with them and then amplifying off that (with nicking enzyme + polymerase *cough*), then they won't be getting consumed after the initial setup | 16:38 |
| nmz787 | de novo 4 life yo | 16:38 |
| * nmz787 back to implementing a pipeline for BRLCAD to g-code | 16:39 | |
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| kanzure | nmz787: linuxcnc probably has something for that already | 17:41 |
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| jrayhawk | http://psybersecurity.tumblr.com/post/144607589631/five-years-later | 18:04 |
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| maaku_ | c0rw1n: or "derp learning" | 19:50 |
| c0rw1n | ? | 19:52 |
| c0rw1n | ( also, lol ) | 19:53 |
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| xentrac_ | nmz787: you may enjoy http://hackaday.com/2016/04/11/continuing-the-dialog-its-time-the-software-people-and-mechanical-people-sat-down-and-had-a-talk/ | 21:32 |
| nmz787 | xentrac_: just gave it a quick once through | 22:07 |
| nmz787 | may touch upon the links later | 22:07 |
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| abetusk | nmz787, any links to the brlcad api/library you re using to export? | 22:35 |
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| --- Log closed Mon May 30 00:00:15 2016 | ||
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